Screening device for cell centrifugation

Abstract

The utility model discloses a screening device for cell centrifugation, which comprises a screening pipe, the top of the screening pipe is provided with a feed port, the bottom of the screening pipe is provided with a waste liquid port, and a first cell screen, a second cell screen, a third cell screen and a fourth cell screen are sequentially arranged in the screening pipe from top to bottom. The screening device for cell centrifugation has the advantages that the screening device is provided with the first cell sieve, the second cell sieve, the third cell sieve and the fourth cell sieve, and accordingly cells with different diameters in cell sap can be sorted by the aid of the first cell sieve, the second cell sieve, the third cell sieve and the fourth cell sieve. The sizes of sieve holes of the cell sieves are sequentially reduced, cells with different diameters can be intercepted step by step, the cell sorting accuracy and efficiency are improved, and the cell sieves can be automatically supported by arranging a first stop lever, a second stop lever, a third stop lever and a fourth stop lever, so that the replacement process of the cell sieves is more convenient and faster.

Description

Technical Field

[0001] The utility model relates to the technical field of cell centrifugation, in particular to a screening device for cell centrifugation. Background Art

[0002] Primary culture is the main way to obtain target cells. Enzyme digestion is used to obtain suspended cell fluid, which contains incompletely digested tissue blocks and various cells. Using a sieve to intercept undigested tissue blocks and sorting cells according to their diameter size is of great significance for obtaining target cells.

[0003] In the prior art, the cell sieve is a cell filtering device with a single pore size, and the bottom of the cell sieve is a sieve. The cell sieve can only retain cells larger than the pore size of the sieve and cannot perform multiple cell sorting.

[0004] For example, a Chinese utility model patent with authorization announcement number CN205386371U discloses a single-cell rapid separation device, including a centrifuge tube, a sleeve is provided inside the centrifuge tube, the sleeve and the centrifuge tube are detachably connected, an upper cover is provided at the upper end of the sleeve, the cover body end is fixedly connected to the sleeve, the other end of the cover body is snap-connected to the opening edge of the sleeve, a cell screen is provided at the bottom of the sleeve, the cell screen is detachably connected to the sleeve, an opening is provided at the bottom of the sleeve, and there is a certain distance between the bottom of the centrifuge tube and the bottom of the sleeve. Utility Model Content

[0005] In order to solve the deficiencies mentioned in the above background technology, the purpose of the utility model is to provide a screening device for cell centrifugation, which realizes the sorting of cells of different diameters in cell fluid by setting a first cell screen, a second cell screen, a third cell screen and a fourth cell screen. The size of the sieve holes of each cell screen is reduced in sequence, and cells of different diameters can be intercepted step by step, thereby improving the accuracy and efficiency of cell sorting, and the first gear rod, the second gear rod, the third gear rod and the fourth gear rod can automatically support the cell screens at each level, making the cell screen replacement process more convenient and quick.

[0006] The purpose of the utility model can be achieved through the following technical solutions:

[0007] A screening device for cell centrifugation comprises a screening tube, wherein a feed port is arranged at the top of the screening tube, a waste liquid port is arranged at the bottom of the screening tube, a first cell sieve, a second cell sieve, a third cell sieve and a fourth cell sieve are arranged in sequence from top to bottom inside the screening tube, and the sieve holes of the first cell sieve, the second cell sieve, the third cell sieve and the fourth cell sieve are successively decreased, and the first cell sieve, the second cell sieve, the third cell sieve and the fourth cell sieve all comprise a separation membrane and a sieve frame arranged at the edge of the separation membrane.

[0008] Further preferably, the sieve tube is symmetrically provided with guide rods, and the sieve frames of the first cell sieve, the second cell sieve, the third cell sieve and the fourth cell sieve are all provided with guide holes adapted to the guide rods. The cooperation between the guide rods and the guide holes enables the cell sieve to maintain a stable position in the sieve tube, preventing displacement or deformation during the centrifugation process.

[0009] Further preferably, a first baffle bar, a second baffle bar, a third baffle bar and a fourth baffle bar are arranged inside the screen tube, and the first baffle bar, the second baffle bar, the third baffle bar and the fourth baffle bar are distributed in a circular array.

[0010] Further preferably, the first baffle rod is located between the first cell sieve and the second cell sieve, the second baffle rod is located between the second cell sieve and the third cell sieve, the third baffle rod is located between the third cell sieve and the fourth cell sieve, and the fourth baffle rod is located below the fourth cell sieve, and the top ends of the first baffle rod, the second baffle rod, the third baffle rod and the fourth baffle rod are respectively abutted and connected with the bottom ends of the first cell sieve, the second cell sieve, the third cell sieve and the fourth cell sieve.

[0011] Further preferably, the sieve frame of the fourth cell sieve is provided with a first through-hole compatible with the first, second and third baffles, the sieve frame of the third cell sieve is provided with a second through-hole compatible with the first and second baffles, and the sieve frame of the second cell sieve is provided with a third through-hole compatible with the first baffle. Since the sieve frame of the fourth cell sieve is provided with a first through-hole compatible with the first, second and third baffles, the third cell sieve can be smoothly moved to the bottom until it is blocked by the fourth baffle, since the sieve frame of the third cell sieve is provided with a second through-hole compatible with the first and second baffles, the third cell sieve can slide down smoothly until it is blocked by the third baffle, since the sieve frame of the second cell sieve is provided with a third through-hole compatible with the first baffle, the second cell sieve can slide down smoothly until it is blocked by the second baffle, and since the first cell sieve is a through-hole, the first cell sieve cannot slide down smoothly and is blocked and supported by the first baffle.

[0012] Further preferably, an upper sealing cover is provided at the feed port of the sieve tube, and a lower sealing cover is provided at the waste liquid port of the sieve tube. The provision of the upper sealing cover and the lower sealing cover ensures the sealing of the sieve device during the centrifugation process and prevents leakage of cell fluid.

[0013] Beneficial effects of the utility model:

[0014] 1. The utility model realizes the sorting of cells of different diameters in the cell fluid by setting the first cell sieve, the second cell sieve, the third cell sieve and the fourth cell sieve. The sieve hole size of each cell sieve decreases successively, and cells of different diameters can be intercepted step by step, thereby improving the accuracy and efficiency of cell sorting. In addition, by setting the first gear lever, the second gear lever, the third gear lever and the fourth gear lever, the cell sieves of each level can be automatically supported, making the cell sieve replacement process more convenient and quick;

[0015] 2. The utility model ensures the sealing of the screening device during the centrifugation process and prevents leakage of cell fluid by arranging the upper sealing cover and the lower sealing cover. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] The utility model is further described below in conjunction with the accompanying drawings.

[0017] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0018] Figure 2 This is a cross-sectional view of the screening tube structure in the utility model;

[0019] Figure 3 It is a schematic diagram of the internal structure of the screening tube in the utility model;

[0020] Figure 4 It is a schematic diagram of the structures of the first cell sieve, the second cell sieve, the third cell sieve and the fourth cell sieve of the utility model.

[0021] In the figure:

[0022] 1. Screening tube; 2. Feed inlet; 3. Waste liquid outlet; 4. First cell screen; 5. Second cell screen; 6. Third cell screen; 7. Fourth cell screen; 8. Separation membrane; 9. Screen frame; 10. Guide rod; 11. Guide hole; 12. First stopper; 13. Second stopper; 14. Third stopper; 15. Fourth stopper; 16. First through-port; 17. Second through-port; 18. Third through-port; 19. Upper sealing cover; 20. Lower sealing cover. DETAILED DESCRIPTION

[0023] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0024] In the description of the present invention, it should be understood that terms such as "opening", "upper", "lower", "thickness", "top", "middle", "length", "inside", "all around" and the like indicating orientation or positional relationship are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the components or elements referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as limiting the present invention.

[0025] like Figure 1-4 As shown, a screening device for cell centrifugation includes a screening tube 1, a feed port 2 is provided at the top of the screening tube 1, a waste liquid port 3 is provided at the bottom of the screening tube 1, and a first cell sieve 4, a second cell sieve 5, a third cell sieve 6 and a fourth cell sieve 7 are provided in sequence from top to bottom inside the screening tube 1, and the sieve holes of the first cell sieve 4, the second cell sieve 5, the third cell sieve 6 and the fourth cell sieve 7 are successively decreased, and the first cell sieve 4, the second cell sieve 5, the third cell sieve 6 and the fourth cell sieve 7 all include a separation membrane 8 and a sieve frame 9 arranged at the edge of the separation membrane 8.

[0026] The interior of the sieve tube 1 is symmetrically provided with guide rods 10, and the sieve frames 9 of the first cell sieve 4, the second cell sieve 5, the third cell sieve 6 and the fourth cell sieve 7 are all provided with guide holes 11 adapted to the guide rods 10. The cooperation between the guide rods 10 and the guide holes 11 enables the cell sieve to maintain a stable position in the sieve tube 1, preventing displacement or deformation during the centrifugation process.

[0027] The screen tube 1 is provided with a first baffle bar 12 , a second baffle bar 13 , a third baffle bar 14 and a fourth baffle bar 15 , which are distributed in a circular array.

[0028] The first baffle rod 12 is located between the first cell sieve 4 and the second cell sieve 5, the second baffle rod 13 is located between the second cell sieve 5 and the third cell sieve 6, the third baffle rod 14 is located between the third cell sieve 6 and the fourth cell sieve 7, and the fourth baffle rod 15 is located below the fourth cell sieve 7. The top ends of the first baffle rod 12, the second baffle rod 13, the third baffle rod 14 and the fourth baffle rod 15 are respectively abutted and connected with the bottom ends of the first cell sieve 4, the second cell sieve 5, the third cell sieve 6 and the fourth cell sieve 7.

[0029] The sieve frame 9 of the fourth cell sieve 7 is provided with a first through-opening 16 adapted to the first baffle 12, the second baffle 13 and the third baffle 14; the sieve frame 9 of the third cell sieve 6 is provided with a second through-opening 17 adapted to the first baffle 12 and the second baffle 13; the sieve frame 9 of the second cell sieve 5 is provided with a third through-opening 18 adapted to the first baffle 12. Since the sieve frame 9 of the fourth cell sieve 7 is provided with a first through-opening 16 adapted to the first baffle 12, the second baffle 13 and the third baffle 14, the third cell sieve 6 can smoothly move to the bottom until it is blocked by the fourth baffle 15. Since the sieve frame 9 of the third cell sieve 6 is provided with a second through-opening 17 adapted to the first baffle 12 and the second baffle 13, the third cell sieve 6 can smoothly slide down until it is blocked by the third baffle 14. Since the sieve frame 9 of the second cell sieve 5 is provided with a third through-opening 18 adapted to the first baffle 12, the second cell sieve 5 can smoothly slide down until it is blocked by the second baffle 13. Since the first cell sieve 4 is a through-opening, the first cell sieve 4 cannot slide down smoothly and is blocked and supported by the first baffle 12.

[0030] The feed port 2 of the sieve tube 1 is provided with an upper sealing cover 19, and the waste liquid port 3 of the sieve tube 1 is provided with a lower sealing cover 20. The provision of the upper sealing cover 19 and the lower sealing cover 20 ensures the sealing of the sieve device during the centrifugation process and prevents leakage of the cell fluid.

[0031] Working principle:

[0032] Open the upper sealing cover 19: Gently unscrew the upper sealing cover 19 on the top of the screening tube 1 to ensure that the sealing surface is not damaged during the operation.

[0033] Adding cell solution: Use appropriate tools (such as a pipette, syringe, etc.) to slowly add the prepared cell solution into the screening tube 1 to avoid bubbles or splashing.

[0034] Close the upper sealing cover 19: After the cell solution is added, tighten the upper sealing cover 19 immediately to ensure the sealing of the sieve tube 1.

[0035] Place the screening device: Place the screening device containing the cell solution steadily into the rotor of the centrifuge, making sure that the screening tube 1 is aligned with the axis of the centrifuge.

[0036] Start the centrifuge: After confirming that the parameter settings are correct, start the centrifuge for centrifugal processing. During the centrifugation process, pay attention to the operating status of the centrifuge to ensure that no abnormalities occur.

[0037] Stop centrifugation: After centrifugation, turn off the power of the centrifuge and wait for the centrifuge to stop rotating completely.

[0038] Open the lower sealing cover 20: Gently unscrew the lower sealing cover 20 at the bottom of the screening tube 1, and be careful to avoid splashing of waste liquid.

[0039] Drain the waste liquid: Place the waste liquid collector under the sieve tube 1 and gently tilt the sieve tube 1 to drain the waste liquid smoothly. After ensuring that the waste liquid is completely drained, proceed to the next step.

[0040] Remove the cell sieves: Remove the cell sieves of each level in turn. Be careful to operate gently to avoid damaging the cells on the cell sieves.

[0041] Collect cells: Collect cells on each level of cell sieve into appropriate containers for subsequent processing or analysis as needed.

[0042] In the description of this specification, the description with reference to the terms "one embodiment", "example", "specific example", etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the utility model. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0043] The above shows and describes the basic principle, main features and advantages of the utility model. Those skilled in the art should understand that the utility model is not limited by the above embodiments, and the above embodiments and descriptions are only for explaining the principle of the utility model. Without departing from the spirit and scope of the utility model, the utility model may have various changes and improvements, and these changes and improvements fall within the scope of the utility model to be protected.

Claims

1. A screening device for cell centrifugation, characterized in that: The invention comprises a sieve tube (1), wherein a feed port (2) is arranged at the top of the sieve tube (1), a waste liquid port (3) is arranged at the bottom of the sieve tube (1), and a first cell sieve (4), a second cell sieve (5), a third cell sieve (6) and a fourth cell sieve (7) are arranged in sequence from top to bottom inside the sieve tube (1), and the sieve holes of the first cell sieve (4), the second cell sieve (5), the third cell sieve (6) and the fourth cell sieve (7) are successively lower, and the first cell sieve (4), the second cell sieve (5), the third cell sieve (6) and the fourth cell sieve (7) all comprise a separation membrane (8) and a sieve frame (9) arranged at the edge of the separation membrane (8).

2. The cell centrifugation screening device according to claim 1, characterized in that: The sieve tube (1) is symmetrically provided with guide rods (10), and the sieve frames (9) of the first cell sieve (4), the second cell sieve (5), the third cell sieve (6) and the fourth cell sieve (7) are all provided with guide holes (11) adapted to the guide rods (10).

3. The cell centrifugation screening device according to claim 1, characterized in that: A first baffle bar (12), a second baffle bar (13), a third baffle bar (14) and a fourth baffle bar (15) are arranged inside the screen pipe (1), and the first baffle bar (12), the second baffle bar (13), the third baffle bar (14) and the fourth baffle bar (15) are distributed in a circular array.

4. The cell centrifugation screening device according to claim 3, characterized in that: The first baffle rod (12) is located between the first cell sieve (4) and the second cell sieve (5), the second baffle rod (13) is located between the second cell sieve (5) and the third cell sieve (6), the third baffle rod (14) is located between the third cell sieve (6) and the fourth cell sieve (7), and the fourth baffle rod (15) is located below the fourth cell sieve (7). The top ends of the first baffle rod (12), the second baffle rod (13), the third baffle rod (14) and the fourth baffle rod (15) are respectively in contact with the bottom ends of the first cell sieve (4), the second cell sieve (5), the third cell sieve (6) and the fourth cell sieve (7).

5. The cell centrifugation screening device according to claim 4, characterized in that: The sieve frame (9) of the fourth cell sieve (7) is provided with a first through-hole (16) adapted to the first baffle rod (12), the second baffle rod (13) and the third baffle rod (14); the sieve frame (9) of the third cell sieve (6) is provided with a second through-hole (17) adapted to the first baffle rod (12) and the second baffle rod (13); and the sieve frame (9) of the second cell sieve (5) is provided with a third through-hole (18) adapted to the first baffle rod (12).

6. The cell centrifugation screening device according to claim 1, characterized in that: An upper sealing cover (19) is provided at the feed port (2) of the sieve pipe (1), and a lower sealing cover (20) is provided at the waste liquid port (3) of the sieve pipe (1).

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