Inoculation device suitable for shake-flask rotary bioreactor

Abstract

The utility model discloses an inoculation device suitable for a shake-flask rotary bioreactor. The inoculation device comprises a funnel, an inoculation barrel and a vacuum pump, a small-caliber liquid leakage opening of the funnel is communicated with the inoculation barrel through a pipeline; the vacuum pump is communicated with the inoculation barrel through a pipeline; the inoculation barrel is provided with an inoculation barrel liquid discharge pipe; according to the utility model, the inoculation process can be ensured to be smoothly carried out, the smooth culture in the fermentation process is ensured, a safe and reliable inoculation device is provided for the fermentation production process, and the device is simultaneously suitable for the inoculation operation of a single reactor and a plurality of reactors.

Description

Technical Field

[0001] The utility model relates to the technical field of biological inoculation, in particular to an inoculation device suitable for a shaking flask-to-bioreactor. Background Art

[0002] In the fields of bioengineering and biomedicine, large-scale bioreactors are often used for fermentation culture for large-scale production of biological products. The inoculation process of the culture is crucial. Improper operation of the inoculation process may cause phage contamination or bacterial contamination, resulting in the inability to carry out the culture and causing losses in the initial investment costs.

[0003] At present, the inoculation process of fermentation culture can be divided into a one-stage amplification inoculation process of shaking flasks to large bioreactors and a multi-stage inoculation process of shaking flasks to small bioreactors and then to large bioreactors according to the different amplification levels. In both inoculation processes, shaking flasks need to be used to transfer reactors. The commonly used inoculation method of shaking flasks to reactors is the flame ring method, that is, a cotton ring containing alcohol is ignited at the inoculation port of the reactor during inoculation, and the seed liquid is poured into the reactor through the inoculation port on the flame. However, this method is difficult and complicated to operate. For the process of multiple shaking flasks to reactors, it takes a long time and is difficult to operate multiple shaking flasks in the open, and it is difficult to completely avoid the risk of contamination. In addition, there is an open flame during the implementation of this method, which poses a great safety hazard. Utility Model Content

[0004] The utility model aims to provide an inoculation device suitable for a shake flask bioreactor to solve the problems raised in the above background technology.

[0005] To achieve the above purpose, the utility model provides the following solutions:

[0006] An inoculation device suitable for a shake flask-to-bioreactor, comprising a funnel, an inoculation bucket and a vacuum pump;

[0007] The small-caliber liquid leakage port of the funnel is connected to the inoculation barrel through a pipeline;

[0008] The vacuum pump is connected to the inoculation barrel through a pipeline;

[0009] The inoculation barrel is provided with an inoculation barrel drain pipe.

[0010] Furthermore, the small-caliber liquid leakage port of the funnel is connected to a funnel liquid outlet pipe, and a funnel liquid outlet quick connector is provided at one end of the funnel liquid outlet pipe away from the funnel;

[0011] The inoculation barrel is connected to an inoculation barrel liquid inlet pipe, and an inoculation barrel liquid inlet quick connector is provided at one end of the inoculation barrel liquid inlet pipe away from the inoculation barrel;

[0012] The funnel liquid outlet quick connector is connected to the inoculation barrel liquid inlet quick connector.

[0013] Furthermore, a vacuum pump connecting pipe is connected to the pump interface of the vacuum pump, an inoculation barrel connecting pipe is connected to the inoculation barrel, and the vacuum pump connecting pipe is connected to the inoculation barrel connecting pipe through an air film.

[0014] Furthermore, a water level detector is provided on the liquid outlet pipe of the funnel, and the water level detector is electrically connected to the vacuum pump via a detection connecting line.

[0015] Furthermore, an inoculation barrel drainage quick connector is provided at one end of the inoculation barrel drainage pipe away from the inoculation barrel.

[0016] Furthermore, it also includes a clean bench and a funnel fixing frame arranged in the clean bench, and the funnel is placed on the funnel fixing frame.

[0017] Furthermore, a three-hole cover is provided on the top of the inoculation barrel, and three connecting holes are opened on the three-hole cover. The three connecting holes are respectively connected to the inoculation barrel, and the inoculation barrel discharge pipe, inoculation barrel inlet pipe and inoculation barrel connecting pipe are respectively connected to the inoculation barrel through the three connecting holes.

[0018] It can be seen from the above technical solutions that, compared with the prior art, the beneficial effects of the present invention are:

[0019] The device adds an inoculation bucket during the inoculation process to inoculate the bacterial solution into the reactor in steps. At the same time, a feedback system is added during the inoculation process through the connection of a water level detector and a vacuum pump to avoid the risk of contamination caused by excessive negative pressure in the inoculation bucket, ensure the smooth progress of the inoculation process, and ensure the smooth cultivation of the fermentation process. The entire process is operated in an ultra-clean workbench, providing a safe and reliable inoculation device and inoculation method for the fermentation production process. The device and inoculation method are suitable for inoculation operations of a single reactor and multiple reactors. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0021] Figure 1 It is a schematic structural diagram of an inoculation device suitable for a shake flask bioreactor according to an embodiment of the utility model.

[0022] Description of reference numerals:

[0023] 1. Vacuum pump; 11. Vacuum pump connecting pipe; 12. Detection connecting line; 2. Inoculation barrel; 21. Three-hole cover; 22. Inoculation barrel connecting pipe; 23. Air film; 24. Inoculation barrel liquid inlet pipe; 25. Inoculation barrel liquid inlet quick connector; 26. Inoculation barrel liquid discharge pipe; 27. Inoculation barrel liquid discharge quick connector; 3. Funnel; 31. Funnel liquid outlet pipe; 32. Funnel liquid outlet quick connector; 4. Water level detector; 5. Funnel fixing bracket; 6. Inoculation shake flask; 7. Clean bench. DETAILED DESCRIPTION

[0024] The following is a further detailed description of the specific implementation of the present invention in conjunction with the accompanying drawings and examples. The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0025] In the description of the present application, it should be understood that the terms "center", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present application.

[0026] The terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of the features. In the description of this application, unless otherwise specified, "plurality" means two or more.

[0027] In the description of this application, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood according to specific circumstances.

[0028] In order to better understand the purpose, structure and function of the present invention, the present invention is further described in detail below in conjunction with the accompanying drawings.

[0029] See also Figure 1As shown, an inoculation device suitable for a shake flask-to-bioreactor of the present embodiment comprises a funnel 3, an inoculation bucket 2, a vacuum pump 1, a clean bench 7 and an inoculation shake flask 6, a funnel fixing frame 5 is placed in the clean bench 7, the funnel 3 is placed on the funnel fixing frame 5, and the funnel 3 is supported, and the clean bench 7 ensures the sterility of the inoculation process;

[0030] The small-diameter leakage port of the funnel 3 is connected to the inoculation barrel 2 through a pipeline, and the vacuum pump 1 is connected to the inoculation barrel 2 through a pipeline. The bacterial solution in the inoculation shake bottle 6 is poured into the funnel 3, and the vacuum pump 1 is turned on to evacuate the inside of the inoculation barrel 2. A negative pressure environment is formed inside the inoculation barrel 2, and the bacterial solution will flow into the inoculation barrel 2 along the pipeline;

[0031] The inoculation barrel 2 is provided with an inoculation barrel drainage pipe 26 , and the inoculation barrel 2 can be connected to the reactor through the inoculation barrel drainage pipe 26 to transfer the bacterial liquid in the inoculation barrel 2 to the reactor for inoculation.

[0032] Specifically, the small-diameter liquid leakage port of the funnel 3 is connected to a funnel liquid outlet pipe 31, and a funnel liquid outlet quick connector 32 is provided at one end of the funnel liquid outlet pipe 31 away from the funnel 3;

[0033] The inoculation barrel 2 is connected to an inoculation barrel liquid inlet pipe 24, and an inoculation barrel liquid inlet quick connector 25 is provided at one end of the inoculation barrel liquid inlet pipe 24 away from the inoculation barrel 2;

[0034] The funnel liquid outlet quick connector 32 is connected to the inoculation barrel liquid inlet quick connector 25, and the inoculation barrel 2 and the funnel 3 are connected in a quick connector manner, which is convenient for installation and disassembly.

[0035] Specifically, the pump interface of the vacuum pump 1 is connected to a vacuum pump connecting pipe 11, and the inoculation barrel 2 is connected to an inoculation barrel connecting pipe 22. The vacuum pump connecting pipe 11 is connected to the inoculation barrel connecting pipe 22 through an air membrane 23. The air membrane 23 can ensure the airtightness of the inoculation barrel 2, and the air membrane 23 is a 0.22μm PTFE air membrane.

[0036] Specifically, a water level detector 4 is provided on the funnel liquid outlet pipe 31, and the water level detector 4 is electrically connected to the vacuum pump 1 through a detection connection line 12. A feedback system is added through the connection between the water level detector 4 and the vacuum pump 1 to avoid the risk of contamination caused by excessive negative pressure in the inoculation barrel 2.

[0037] Specifically, an inoculation barrel drainage quick connector 27 is provided at one end of the inoculation barrel drainage pipe 26 away from the inoculation barrel 2 .

[0038] Specifically, a three-hole cover 21 is provided on the top of the inoculation barrel 2, and three connecting holes are opened on the three-hole cover, and the three connecting holes are respectively connected to the inoculation barrel 2, and the inoculation barrel discharge pipe 26, the inoculation barrel inlet pipe 24 and the inoculation barrel connecting pipe 22 are respectively connected to the inoculation barrel 2 through the three connecting holes.

[0039] The specific working process of the device is as follows: when the bacterial liquid in the inoculation shake flask 6 is poured into the funnel 3, the water level detector 4 on the funnel outlet pipe 31 detects the presence of liquid and feeds back to the vacuum pump 1, the vacuum pump 1 starts to work, and the air in the inoculation barrel 2 is extracted through the vacuum pump connecting pipe 11, so that a negative pressure is formed in the inoculation barrel 2. Under the action of atmospheric pressure, the bacterial liquid in the funnel 3 will be pressed into the inoculation barrel 2, thereby completing the operation of transferring the bacterial liquid from the inoculation shake flask 6 to the inoculation barrel 2. After all the bacterial liquid flows into the inoculation barrel 2, the water level detector 4 detects that no liquid passes through, and feeds back to the vacuum pump 1, so that the vacuum pump 1 stops working, thereby preventing the risk of bacterial infection caused by excessive negative pressure in the inoculation barrel 2;

[0040] After the transfer of the inoculation barrel 2 is completed, the inoculation barrel 2 can be connected to the reactor through the inoculation barrel discharge quick connector 27. At this time, gas is filled into the inoculation barrel 2 through the air membrane 23 to form a positive pressure in the inoculation barrel 2, so that the bacterial liquid enters the reactor through the inoculation barrel discharge pipe 26, thereby completing the inoculation process from the inoculation shake flask 6 to the reactor;

[0041] In addition, for the fermentation process of inoculating multiple reactors at the same time, the inoculation barrel 2 can be connected to the reactor by disassembling the inoculation barrel discharge quick connector 27, and then connected to the quick connector of the other reactor after disassembly. By filling the inoculation barrel 2 with gas, the bacteria can be pressed into another reactor to perform the inoculation operation of the other reactor.

[0042] This article uses specific examples to illustrate the principles and implementation methods of the utility model. The above examples are only used to help understand the method and core ideas of the utility model. At the same time, for those skilled in the art, according to the ideas of the utility model, there will be changes in the specific implementation methods and application scope. In summary, the content of this specification should not be understood as limiting the utility model.

Claims

1. An inoculation device suitable for a shake flask bioreactor, characterized in that: Includes funnel, inoculation bucket and vacuum pump; The small-caliber liquid leakage port of the funnel is connected to the inoculation barrel through a pipeline; The vacuum pump is connected to the inoculation barrel through a pipeline; The inoculation barrel is provided with an inoculation barrel drain pipe.

2. An inoculation device suitable for a shake flask-to-bioreactor according to claim 1, characterized in that: The small-caliber liquid leakage port of the funnel is connected to a funnel liquid outlet pipe, and a funnel liquid outlet quick connector is provided at one end of the funnel liquid outlet pipe away from the funnel; The inoculation barrel is connected to an inoculation barrel liquid inlet pipe, and an inoculation barrel liquid inlet quick connector is provided at one end of the inoculation barrel liquid inlet pipe away from the inoculation barrel; The funnel liquid outlet quick connector is connected to the inoculation barrel liquid inlet quick connector.

3. The inoculation device suitable for a shake flask-to-bioreactor according to claim 2, characterized in that: A vacuum pump connecting pipe is connected to the pump interface of the vacuum pump, an inoculation barrel connecting pipe is connected to the inoculation barrel, and the vacuum pump connecting pipe is connected to the inoculation barrel connecting pipe through an air film.

4. The inoculation device suitable for a shake flask-to-bioreactor according to claim 2, characterized in that: A water level detector is provided on the funnel liquid outlet pipe, and the water level detector is electrically connected to the vacuum pump through a detection connection line.

5. The inoculation device suitable for a shake flask-to-bioreactor according to claim 1, characterized in that: An inoculation barrel drainage quick connector is arranged at one end of the inoculation barrel drainage pipe away from the inoculation barrel.

6. The inoculation device suitable for a shake flask-to-bioreactor according to claim 1, characterized in that: It also includes an ultra-clean workbench and a funnel fixing frame arranged in the ultra-clean workbench, and the funnel is placed on the funnel fixing frame.

7. The inoculation device suitable for a shake flask-to-bioreactor according to claim 3, characterized in that: A three-hole cover is provided on the top of the inoculation barrel, and three connecting holes are opened on the three-hole cover. The three connecting holes are respectively connected to the inoculation barrel, and the inoculation barrel discharge pipe, inoculation barrel inlet pipe and inoculation barrel connecting pipe are respectively connected to the inoculation barrel through the three connecting holes.

Images