Fish lens induction defocusing experiment device

Abstract

The utility model discloses a fish lens induction defocusing experiment device, including: lens induction defocusing culture unit, connecting water pipe and water storage tank wherein the lens induction defocusing culture unit includes: box body, lens support and paired lens wherein the box body is used for holding fish and fish culture water, and the box body is used for holding fish and fish culture water. The lens support is used for fixing the paired lenses on the two sides of the fish and making the paired lenses close to the fish eyes. The connecting water pipe is used for connecting the two ends of the box body with the water storage tank. According to the device, the lens induction defocusing culture unit is arranged on the basis of the physiological characteristics of the fish, the fish is cultured in the box body of the lens induction defocusing culture unit, and the lenses are arranged on the two sides of the fish eye, so that the lens induction defocusing of the eyes of the fish can be realized, and an eye suture lens operation does not need to be performed on the fish; the device has the advantages of simple structure, easiness in assembly, biological friendliness and the like.

Description

Technical Field

[0001] The utility model relates to the technical field of animal experimental equipment, in particular to a fish lens induced defocusing experimental device. Background Art

[0002] Refractive error refers to a problem in the eye's ability to focus light to the retina, resulting in blurred or unclear vision. This may be due to an incorrect shape of the eyeball, the curvature of the cornea or lens, or a mismatch between the length of the eyeball and the refractive power. Common refractive errors include myopia (nearsightedness), hyperopia (hyperopia), astigmatism, etc. Among them, myopia means that the eye's focus on distant objects falls in front of the retina, causing distant objects to appear blurry. This is usually caused by the eyeball's axis being longer than normal or the cornea being too curved. Hyperopia means that the eye's focus on nearby objects falls behind the retina, causing nearby objects to appear blurry. Hyperopia is usually caused by the eyeball's axis being too short or the cornea being not curved enough.

[0003] Human epidemiological and genetic studies are essential for understanding the causes of normal and abnormal eye development, and animal models provide an observational research approach in this regard. As one of the five fish experimental animals recognized by the International Organization for Standardization, zebrafish plays an indispensable role in scientific research. The eye development process of zebrafish presents a high degree of molecular complexity and strict spatiotemporal regulation, which is highly similar to the development of human eyeballs. Zebrafish has a high degree of genetic similarity with humans, and its low cost makes it more advantageous than common rodent and cell models. Its embryos are transparent and large in number, which can support experiments with hundreds of samples at the same time and facilitate observation by researchers. Compared with traditional mammalian models, zebrafish as an experimental animal model provides many advantages for visual research: First, zebrafish are diurnal animals with four-color vision and a retina rich in cones, which can highly simulate the visual system mediated mainly by cones in the human retina. Secondly, Morpholino, TALEN and CRISPR / Cas9 technologies can be used to modify zebrafish genes relatively easily. The convenience of genome manipulation helps to quickly screen mutant lines and then measure changes in axial length and other eye biomarkers. In summary, zebrafish, as one of the classic vertebrate models, is highly favored by ophthalmologists and provides a valuable research platform for studying human eye development and diseases.

[0004] The principle of lens-induced defocus (LID) is as follows: Figure 1As shown, the LID myopia / hyperopia model is established by fixing the negative lens / positive lens in front of the animal's eyes using eye masks, face masks, surgically sutured lenses, etc. The successful establishment of the LID method has greatly promoted the pace of molecular biology research on myopia / hyperopia. Initially, Wildsoet used positive lenses to simulate artificial myopia on chickens and found that it would cause thickening of the chicken choroid (i.e., the retina moves forward) and slow down the overall growth of the eyeball, leading to hyperopic refractive error. On the contrary, simulating hyperopic defocus by using negative lenses will lead to artificial hyperopia, which will cause thinning of the choroid (i.e., the retina moves backward) and increase the growth of the eyeball, thereby promoting the establishment of a myopic state. At present, the LID method has been successful in model organisms such as chickens, guinea pigs, guinea pigs, mice, etc. However, since zebrafish live in an aquatic environment and their skin is covered with scales, the volume of zebrafish eyeballs is much smaller than that of model organisms such as mice, guinea pigs, and chickens (the eye axis of zebrafish fry on the fifth day after fertilization is usually between 200 μm and 500 μm), so existing methods of fixing lenses such as eye patches, face masks, and surgical suture of lenses are not effective. Specifically, the existing technologies have the following defects:

[0005] (1) The lens device needs to be fixed to the surface of the fish eye by suturing, which will cause damage to the fish body. The wound after suturing can easily cause infection in the fish and even cause the death of the fish.

[0006] (2) It is difficult to sew and fix the lens device on fish, which requires a lot of sew experience. The smaller the fish, the more difficult it is to tolerate and implement the lens device sew. In addition, if the sew position is not accurate, corneal astigmatism is likely to occur.

[0007] (3) Juvenile fish are extremely small. Five days after fertilization, the axial length of the zebrafish eye is only between 200 μm and 500 μm. Existing surgical instruments and needles are unable to safely suture and fix the lens.

[0008] (4) As fish grow from juvenile to adult, their body size changes dramatically (up to hundreds of times), resulting in a lens that can only be used for a short period of time before it no longer fits the fish's eye. The lens needs to be re-stitched and replaced with a lens that matches the eye size, which is bound to cause further damage.

[0009] Therefore, it is urgent to establish a lens-induced defocus experimental device suitable for fish. Utility Model Content

[0010] In view of this, the utility model proposes a fish lens induced defocusing experimental device, which can solve the above existing technical problems. The utility model provides the following technical solutions:

[0011] A fish lens-induced defocus experimental device, a lens-induced defocus cultivation unit, a connecting water pipe and a water storage tank, wherein the lens-induced defocus cultivation unit comprises: a box body, a lens bracket and a pair of lenses, wherein the box body is used to accommodate fish and fish cultivation water, the lens bracket is used to fix the pair of lenses on both sides of the fish and close to the fish eyes respectively; the connecting water pipe is used to connect the two ends of the box body to the water storage tank respectively.

[0012] Optionally, the lens has a power range of -30D to +30D.

[0013] Optionally, the distance between the paired lenses is D, and the eye distance of the fish is d, wherein: 10 μm≤(Dd)≤100 μm.

[0014] Optionally, the spacing between the paired lenses is adjustable.

[0015] Optionally, there are multiple lens-induced defocus culture units, and the multiple lens-induced defocus culture units are connected in series.

[0016] Optionally, the water tank comprises: a box body and a water pump, wherein: the box body is used to store the fish culture water; the water pump is used to form a water flow path, and the water flow path refers to the fish culture water in the box body being pumped to the box body inlet of the lens induced defocus culture unit, and then flowing through the box body and out from the box body outlet, and then returning to the box body.

[0017] Optionally, the water storage tank further comprises a water quality filtration unit, and the water quality filtration unit is arranged on the water flow passage finger, and is used for filtering out impurities in the fish culture water.

[0018] Optionally, the water storage tank also includes one or more combinations of a water acid-base balance unit, a water salt balance unit, a temperature balance unit, a water level balance unit, and a water oxygen balance unit, wherein the water acid-base balance unit is used to maintain the acid-base balance of the fish culture water body; the water salt balance unit is used to maintain the salt balance of the fish culture water body; the temperature balance unit is used to maintain the temperature balance of the fish culture water body; the water level balance unit is used to maintain the water level balance of the fish culture water body; and the water oxygen balance unit is used to maintain the water oxygen balance of the fish culture water body.

[0019] Optionally, the water storage tank further comprises a numerical control panel, wherein the numerical control panel is connected to one or more of the water quality acid-base balance unit, the water quality salt balance unit, the temperature balance unit, the water level balance unit, and the water oxygen balance unit.

[0020] Optionally, the fish is zebrafish.

[0021] According to the technical solution of the utility model, a lens induced defocus culture unit is designed based on the physiological characteristics of fish. Fish are cultured in the box of the lens induced defocus culture unit, and lenses are arranged on both sides of the fish eyes. Therefore, lens induced defocusing of the fish eyes can be achieved without the need to perform eye suture lens surgery on the fish. The utility model has the advantages of simple structure, easy assembly, and bio-friendliness. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] For the purpose of illustration and not limitation, the present invention will now be described according to its preferred embodiments, in particular with reference to the accompanying drawings, in which:

[0023] Figure 1 This is a schematic diagram of the principle of the eye defocus induction experiment;

[0024] Figure 2 This is a structural block diagram of a fish lens induced defocusing experimental device according to an embodiment of the utility model;

[0025] Figure 3A This is a schematic front view of the lens-induced defocus culture unit of the utility model containing fish;

[0026] Figure 3B A schematic cross-sectional view of a lens-induced defocusing culture unit of the utility model containing fish and selecting a pair of lenses for a flat mirror;

[0027] Figure 3C Another cross-sectional schematic diagram of the lens-induced defocusing culture unit of the utility model containing fish and selecting a flat lens as the lens;

[0028] Figure 3D Another cross-sectional schematic diagram of the lens-induced defocusing culture unit of the utility model containing fish and selecting a flat lens as the lens;

[0029] Figure 3E A schematic cross-sectional view of a lens-induced defocusing culture unit of the utility model containing fish and selecting a convex lens as a pair of lenses;

[0030] Figure 3F A schematic cross-sectional view of a lens-induced defocusing culture unit of the utility model containing fish and selecting concave lenses as pairs of lenses;

[0031] Figure 4 The present invention is a structural block diagram of a water tank of a fish lens induced defocus experimental device according to an embodiment of the present invention. DETAILED DESCRIPTION

[0032] The technical solution of the utility model will be described clearly and completely below in conjunction with the accompanying drawings. Obviously, the described embodiments are part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0033] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms such as "installation" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0034] like Figure 2 As shown, the fish lens induced defocus experimental device 01 according to the embodiment of the utility model mainly includes: a lens induced defocus cultivation unit 10, a connecting water pipe 20 and a water storage tank 30.

[0035] The lens induced defocusing culture unit 10 is used to accommodate fish and perform lens induced defocusing culture on the fish. The fish is preferably zebrafish. Healthy zebrafish have good balance ability. They have a natural tendency to keep their bodies horizontal as much as possible in water to maintain stability. The eyes of zebrafish are not located in front of the head like mammals such as humans, mice, and guinea pigs, but are located on both sides of the body.

[0036] The number of the lens-induced defocusing culture units 10 can be multiple, and multiple lens-induced defocusing culture units 10 can be connected in series. In this way, multiple experiments can be carried out at the same time, thereby improving the experimental efficiency.

[0037] The lens induced defocus culture unit 10 mainly includes a box body 101, a lens holder 102 and a pair of lenses 103. The box body 101 is used to accommodate fish and fish culture water. The lens holder 102 is used to fix the pair of lenses 103 on both sides of the fish and close to the fish eyes. The box body 101 can be made of a slightly elastic material such as rubber, which can increase the friction between it and the lens, which is more conducive to fixing the lens.

[0038] The distance between the paired lenses 103 is D, and the distance between the eyes of the fish is d, where: 10 μm ≤ (Dd) ≤ 100 μm. This means that the lens is almost close to the fish's eyes but does not press the fish's eyes, which can better achieve the induced defocus experiment.

[0039] Since fish will gradually grow up, it is preferred that the spacing between the paired lenses 103 is adjustable. In this way, it is not necessary to frequently move the same experimental fish to different sizes of lens-induced defocusing culture units 10 for culture. Instead, the spacing D between the paired lenses 103 is adjusted so that the lens spacing matches the eye spacing of the fish, which has the advantages of simple operation, saving culture containers, and low cost. The specific method can be to manually adjust the spacing between the lenses 103 and then fix them with the lens holder 102.

[0040] Figure 3A This is a schematic diagram of the front view of the lens-induced defocused culture unit of the present invention for accommodating fish. It should be explained that, in order to distinguish the transparent components from each other, the fish culture water inside the box body 101 is colored gray, the paired lenses 103 are colored light blue, and the rear FIG. 3B to FIG. 3F Do the same coloring process.

[0041] Figure 3B The schematic cross-sectional view of the lens-induced defocusing culture unit 10 of the present invention accommodating fish and selecting a flat mirror as the paired lens 103 .

[0042] Figure 3C This is another cross-sectional schematic diagram of the lens induced defocusing culture unit 10 of the present invention accommodating fish and selecting flat lenses as the paired lenses 103 . Figure 3C and Figure 3B In comparison, the size of the experimental fish became larger and the spacing between the flat lenses became wider.

[0043] Figure 3D This is another cross-sectional schematic diagram of the lens-induced defocusing culture unit 10 of the present invention accommodating fish and selecting a flat lens as the lens. Figure 3D and Figure 3B In comparison, a mesh 104 is added above the box body 101, which can prevent foreign debris from falling into the box body 101 on the one hand, and prevent the experimental fish from jumping out of the box body 101 on the other hand. The mesh 104 is optional and not necessary.

[0044] Figure 3E The schematic cross-sectional view of the lens induced defocusing culture unit 10 of the present invention accommodating fish and selecting convex lenses as the paired lenses 103 . Figure 3E and Figure 3D In comparison, only the type of lens has been changed.

[0045] Figure 3F The schematic cross-sectional view of the lens induced defocusing culture unit 10 of the present invention accommodating fish and selecting concave lenses as the paired lenses 103 . Figure 3F and Figure 3D In comparison, only the type of lens has been changed.

[0046] The connecting water pipe 20 is used to connect the two ends of the box body 101 to the water storage tank 30 respectively, so that a complete water circuit can be formed.

[0047] The paired lenses 103 can also be called lens groups, which can change the focus position of external incident light in the fish's eyes, thereby achieving the purpose of optical defocus. The spacing between the paired lenses 103 is as close to the surface of the fish's eyes as possible, but does not squeeze or touch the eyes, so as to achieve the purpose of wearing lenses for fish. The lenses can be cleaned regularly to prevent the clarity of the equipment from being reduced.

[0048] The lens 103 may be a flat lens, a convex lens or a concave lens, so that different induced defocus effects can be achieved. The power of the lens 103 ranges from -30D to +30D. For example, 0D, +1D, +2D, +3D, +4D, +5D, +10D, +15D, +20D, +25D, +30D, -1D, -2D, -3D, -4D, -5D, -10D, -15D, -20D, -25D, -30D, etc.

[0049] like Figure 4 As shown, the water storage tank 30 may include a box body 301 and a water pump 302. The box body 301 is used to contain the fish culture water; the water pump 302 is used to form a water flow path. The water flow path refers to the fish culture water in the box body being pumped to the box body inlet of the lens induced defocus culture unit 10, then flowing through the box body 101 and out of the box body outlet, and then returning to the box body 301.

[0050] The water storage tank 30 also includes a water quality filter unit 303, which is arranged on the water flow passage finger and is used to filter out impurities in the fish culture water. An ultraviolet biochemical filter module can also be arranged in the water quality filter unit 303 to achieve the purpose of sterilization.

[0051] The water storage tank 30 also includes a combination of one or more of a water quality acid-base balance unit 304 , a water quality salt balance unit 305 , a temperature balance unit 306 , a water level balance unit 307 , and a water oxygen balance unit 308 .

[0052] The water quality acid-base balance unit 304 is used to maintain the acid-base balance of the fish culture water. When the pH value of the fish culture water is detected to be lower than the set standard, the water quality acid-base balance unit 304 can extract the sodium bicarbonate solution in the sodium bicarbonate solution storage bottle into the box 301. When the pH value is detected to be higher than the set standard, the pure water valve is controlled to open and water is introduced into the box 301 through the water inlet pipe to dilute the pH value.

[0053] The water quality salt balance unit 305 is used to maintain the salt balance of the fish culture water. When the salt concentration of the fish culture water is detected to be lower than the set standard, the water quality salt balance unit 305 can extract the sodium chloride solution in the sodium chloride solution storage bottle into the box 301. When the salinity is detected to be higher than the set standard, the pure water valve is controlled to open and water is introduced into the box 301 through the water inlet pipe to dilute the salinity.

[0054] The temperature balance unit 306 is used to maintain the temperature balance of the fish culture water body. The temperature balance unit 306 may include a temperature sensor and a heater, which are linked together.

[0055] The water level balance unit 307 is used to maintain the water level balance of the fish culture water body. The water level balance unit 307 generally includes a water level sensor, which can be linked with the water inlet valve and the drain outlet switch of the water storage tank 30.

[0056] The water oxygen balance unit 308 is used to maintain the water oxygen balance of the fish culture water body. The water oxygen balance unit 308 may include a water oxygen test sensor and an oxygen production unit. When the water oxygen test sensor detects that the water oxygen content is low, the oxygen production unit is started to supplement oxygen in the water body.

[0057] The water storage tank 30 also includes a numerical control panel 309. The numerical control panel 309 is connected to one or more of the water quality acid-base balance unit 304, the water quality salt balance unit 305, the temperature balance unit 306, the water level balance unit 307, and the water oxygen balance unit 308. The numerical control panel 309 can display the target temperature, current temperature, target pH, current pH, target salinity, current salinity, target oxygen content, and current oxygen content. The specific values ​​of the target temperature, target pH, target salinity, and target oxygen saturation can be adjusted by the buttons of the numerical control panel 309. When these indicators reach the corresponding preset thresholds, an alarm signal is displayed. The alarm signal can be an exclamation mark displayed on the display screen, or accompanied by a buzzer sound, etc.

[0058] It can be seen from the above that the fish lens induced defocus experimental device of the utility model has at least the following advantages:

[0059] (1) A non-contact lens is used to induce defocus in the fish's eyes. The device eliminates the difficult operation of suturing and fixing the lens device on the fish's eyes, reduces the reliance on rich experience, and thus reduces the risk of damage to the fish.

[0060] (2) The present invention is applicable not only to adult fish but also to juvenile fish, thus solving the problem that the existing technology cannot safely sew and fix the lens on very small juvenile fish.

[0061] (3) By adjusting the distance between paired lenses, the eye size of the same experimental fish at different developmental stages can be flexibly adapted, thereby avoiding damage to the eye and surrounding tissues caused by repeated suturing and replacement of lenses.

[0062] (4) It has increased the diversity of methods for inducing refractive errors and promoted scientific research and development in the field of optometry.

[0063] The above specific implementations do not constitute a limitation on the protection scope of the present utility model. It should be understood by those skilled in the art that various modifications, combinations, sub-combinations and substitutions may occur depending on design requirements and other factors. Any modification, equivalent substitution and improvement made within the spirit and principle of the present utility model shall be included in the protection scope of the present utility model.

Claims

1. A fish lens induced defocus experimental device, characterized in that: include: Lens-induced defocusing culture unit, connecting water pipe and water storage tank, wherein, The lens induced defocus culture unit comprises: a box body, a lens holder and a pair of lenses, wherein the box body is used to contain fish and fish culture water, and the lens holder is used to fix the pair of lenses on both sides of the fish and close to the fish eyes; The connecting water pipe is used to connect the two ends of the box body to the water tank respectively.

2. The fish lens induced defocus experimental device according to claim 1, characterized in that: The lens has a power range of -30D to +30D.

3. The fish lens induced defocus experimental device according to claim 1, characterized in that: The distance between the paired lenses is D, and the distance between the eyes of the fish is d, wherein: 10 μm≤(Dd)≤100 μm.

4. The fish lens induced defocusing experimental device according to claim 1, characterized in that: The distance between the paired lenses is adjustable.

5. The fish lens induced defocusing experimental device according to claim 1, characterized in that: The number of the lens induced defocus culture units is multiple, and the multiple lens induced defocus culture units are connected in series.

6. The fish lens induced defocusing experimental device according to claim 1, characterized in that: The water storage tank comprises: a tank body and a water pump, wherein: The box is used to store the fish culture water; The water pump is used to form a water flow path, which means that the fish culture water in the box is pumped to the box inlet of the lens induced defocus culture unit, then flows through the box, flows out from the box outlet, and then returns to the box.

7. The fish lens induced defocusing experimental device according to claim 6, characterized in that: The water storage tank also includes a water quality filter unit, which is arranged on the water flow passage finger and is used to filter out impurities in the fish culture water.

8. The fish lens induced defocusing experimental device according to claim 1, characterized in that: The water storage tank also includes one or more of a water acid-base balance unit, a water salt balance unit, a temperature balance unit, a water level balance unit, and a water oxygen balance unit, wherein: The water quality acid-base balance unit is used to maintain the acid-base balance of the fish culture water; The water quality salt balance unit is used to maintain the salt balance of the fish culture water body; The temperature balance unit is used to maintain the temperature balance of the fish culture water body; The water level balance unit is used to maintain the water level balance of the fish culture water body; The water-oxygen balance unit is used to maintain the water-oxygen balance of the fish culture water body.

9. The fish lens induced defocusing experimental device according to claim 8, characterized in that: The water storage tank also includes a numerical control panel, wherein the numerical control panel is connected to one or more of the water quality acid-base balance unit, the water quality salt balance unit, the temperature balance unit, the water level balance unit, and the water oxygen balance unit.

10. The fish lens induced defocus experimental device according to any one of claims 1 to 9, characterized in that: The fish is zebrafish.

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