Escherichia coli phage screening device

Abstract

The utility model discloses an Escherichia coli bacteriophage screening device, relates to the technical field of Escherichia coli bacteriophage screening, and aims to solve the problems that when the conventional Escherichia coli bacteriophage is screened by a filtering method, the Escherichia coli bacteriophage is mixed and splashes in a vortex manner, so that residues are caused on a higher inner wall of a mixing tank, long-time precipitation is required, and the efficiency is low. A sealing cover is arranged above the mixing tank, and a discharging pipe is arranged at the lower end of the mixing tank; the device comprises a mixing tank, and further comprises a limiting baffle arranged in the mixing tank, four fixing plates are arranged on the upper surface of the limiting baffle, fixing screws are arranged on one sides of the fixing plates, and the fixing plates are in threaded connection with the mixing tank through the fixing screws; and the flexible plates are arranged below the limiting baffles, fixing bases are arranged at the lower ends of the flexible plates, the number of the flexible plates is four, the number of the fixing bases is four, and threaded fasteners are arranged on the two sides of each fixing base.

Description

Technical Field

[0001] The utility model relates to the technical field of Escherichia coli phage screening, in particular to an Escherichia coli phage screening device. Background Art

[0002] Escherichia coli phage refers to a type of phage that parasitizes Escherichia coli and is a type of cell virus. Once it leaves the host cell, it cannot replicate or grow. Bacteriophages are widely distributed in nature. Wherever there are bacteria, there may be corresponding phages. Therefore, phages may be isolated from seawater, fresh water, sewage, and even sea mud or seafood. The commonly used screening method is filtration.

[0003] For example, the Chinese patent with announcement number CN214346204U (a bacteriophage screening device for pathogenic bacteria in sludge) comprises a mixing drum, a drum cover installed at the upper end of the mixing drum, a motor installed at the middle position of the upper surface of the drum cover, and a mixing shaft installed at the output end of the motor and extending into the mixing drum, wherein a plurality of mixing rods are evenly installed at one end of the mixing shaft at the lower position in the mixing drum, a branch pipe is installed at the middle position of the outer surface of the mixing drum, a stop valve is installed at the end of the branch pipe away from the mixing drum, a through opening is opened on the upper surface of the drum cover, a circular hole is provided on one side of the through opening, a vertically arranged threaded rod is provided in the circular hole, a first nut threadedly connected to the threaded rod is provided on the upper and lower sides of the circular hole, and a scraper is installed at the lower end of the threaded rod. Compared with the prior art, the utility model has the following beneficial effects: it enables the sludge sample to be smoothly added to the water in the mixing drum, and prevents the mixing drum mouth from being contaminated;

[0004] For example, the Chinese patent with announcement number CN217709444U (a filtering and diluting device for bacteriophages): includes a dilution box, a first cavity and a second cavity, a stirring mechanism is arranged inside the dilution box, a second cavity is installed on one side of the top of the dilution box, a feed channel is installed at the bottom of the second cavity, and a solenoid valve is installed at one end of the feed channel, a filtering structure is arranged inside the second cavity, a first cavity is installed on one side of the second cavity, a water pump is arranged on one side of the first cavity, and liquid delivery pipes are installed at the top and bottom of the water pump. The utility model uses a weighing sensor arranged at the bottom of the first cavity and the second cavity, and the weighing sensor can weigh the liquid on the weighing plate and transmit the information to the single-chip microcomputer. When the weight of the liquid reaches the set value, the single-chip microcomputer processes the information and transmits it to the alarm, and the alarm is activated to achieve the purpose of the alarm and remind the workers to stop adding materials.

[0005] However, when the existing Escherichia coli phage is screened by filtration, vortex splashing occurs during mixing, resulting in residues on the higher inner wall of the mixing tank, requiring a long time to settle, resulting in low efficiency; therefore, it does not meet existing needs. In this regard, we propose an Escherichia coli phage screening device. Utility Model Content

[0006] The purpose of the utility model is to provide an Escherichia coli phage screening device to solve the problem in the above-mentioned background technology that when the existing Escherichia coli phage is screened by filtration, vortex splashing occurs during mixing, resulting in residues on the higher inner wall of the mixing tank, requiring a long time for precipitation, and resulting in low efficiency.

[0007] To achieve the above object, the utility model provides the following technical solution: an E. coli phage screening device, comprising: a mixing tank, a sealing cover is provided on the top of the mixing tank, and a discharge pipe is provided at the lower end of the mixing tank;

[0008] Also includes:

[0009] A limit baffle is arranged inside the mixing tank, a fixing plate is arranged on the upper surface of the limit baffle, and four fixing plates are arranged, a fixing screw is arranged on one side of the fixing plate, and the fixing plate is threadedly connected to the mixing tank through the fixing screw;

[0010] A tough plate is arranged below the limit baffle, a fixed base is arranged at the lower end of the tough plate, and four tough plates and four fixed bases are arranged.

[0011] Preferably, threaded fasteners are provided on both sides of the fixed base, and the fixed base is threadedly connected to the mixing tank through the threaded fasteners.

[0012] Preferably, a coupling is provided at the upper end of the sealing cover, a stirring motor is provided at the upper end of the coupling, and a mixing shaft is provided at the lower end of the coupling.

[0013] Preferably, a connecting seat is provided on the outside of the mixing shaft, mixing blades are provided on both sides of the connecting seat, a single-head fastener is provided at the front end of the connecting seat, and the connecting seat is threadedly connected to the mixing shaft via the single-head fastener.

[0014] Preferably, a feed pipe is provided on one side of the coupling, and the feed pipe and the sealing cover are an integral structure, and the interior of the feed pipe passes through the sealing cover.

[0015] Preferably, a fixing flange is provided at the connection between the sealing cover and the mixing tank, and fixing bolts are provided at the upper ends of the four sides of the fixing flange.

[0016] Preferably, support legs are provided on the lower surface of the four sides of the mixing tank, and the support legs are welded and fixed to the mixing tank, and a solenoid valve is provided at the front end of the discharge pipe.

[0017] Compared with the prior art, the beneficial effects of the utility model are:

[0018] 1. The utility model sets a limit baffle inside the mixing tank. When stirring and mixing, the mixing blades are used to rotate, and the materials rotate in a vortex manner. At the same time, when the vortex material splashes upward at the edge, it is limited and blocked by the limit baffle, thereby avoiding splashing upward, reducing residue, and saving sedimentation time. The utility model solves the problem that when the existing Escherichia coli phage is screened by filtration, vortex splashing occurs during mixing, resulting in residue on the higher inner wall of the mixing tank, requiring a long time to sediment, and resulting in low efficiency. A single-head fastener is set at the front end of the connecting seat, and the connecting seat is threadedly connected to the mixing shaft through the single-head fastener, which is convenient for disassembly and replacement of the mixing blades, and improves flexibility and convenience.

[0019] 2. By setting a tough plate inside the mixing tank, when stirring and mixing, the mixing blades are rotated. When passing through the tough plate, the tough plate is bent by force and then rebounds. The rebound improves the mixing effect of the bottom material and improves the mixing efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0021] Figure 2 This is a schematic diagram of the internal structure of the mixing tank of the utility model;

[0022] Figure 3 This is a schematic diagram of the internal structure of the mixing tank of the utility model from a top view;

[0023] Figure 4 This is a schematic diagram of the toughness plate structure of the utility model;

[0024] In the figure: 1. Mixing tank; 2. Sealing cover; 3. Fixed flange; 4. Feed pipe; 5. Stirring motor; 6. Coupling; 7. Discharge pipe; 8. Support leg; 9. Mixing shaft; 10. Mixing blade; 11. Connecting seat; 12. Single-head fastener; 13. Limit baffle; 14. Fixed plate; 15. Fixed screw; 16. Tough plate; 17. Fixed base; 18. Threaded fastener; 19. Fixed bolt; 20. Solenoid valve. DETAILED DESCRIPTION

[0025] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments.

[0026] See also Figure 1-4 The utility model provides an embodiment: an Escherichia coli phage screening device, comprising: a mixing tank 1, a sealing cover 2 is arranged above the mixing tank 1, and a discharge pipe 7 is arranged at the lower end of the mixing tank 1;

[0027] Also includes:

[0028] A limit baffle 13 is arranged inside the mixing tank 1. A fixing plate 14 is arranged on the upper surface of the limit baffle 13. Four fixing plates 14 are arranged. A fixing screw 15 is arranged on one side of the fixing plate 14. The fixing plate 14 is threadedly connected to the mixing tank 1 through the fixing screw 15.

[0029] The tough plate 16 is disposed below the limit baffle 13 . A fixed base 17 is disposed at the lower end of the tough plate 16 . Four tough plates 16 and four fixed bases 17 are disposed.

[0030] During stirring and mixing, the mixing blade 10 is rotated, and when passing through the tough plate 16, the tough plate 16 is bent by force, and then rebounds. The rebound improves the mixing effect of the bottom material and the mixing efficiency. At the same time, when the vortex material splashes upward at the edge, it is limited and blocked by the limit baffle 13, thereby avoiding splashing upward, reducing residue, and saving sedimentation time.

[0031] See also Figure 4 Threaded fasteners 18 are provided on both sides of the fixed base 17, and the fixed base 17 is threadedly connected to the mixing tank 1 through the threaded fasteners 18 to fix the fixed base 17.

[0032] See also Figure 1 , 2 A coupling 6 is disposed at the upper end of the sealing cover 2, a stirring motor 5 is disposed at the upper end of the coupling 6, and a mixing shaft 9 is disposed at the lower end of the coupling 6 to realize the rotation of the mixing shaft 9.

[0033] See also Figure 1 , 2A connecting seat 11 is provided on the outside of the mixing shaft 9, and mixing blades 10 are provided on both sides of the connecting seat 11. A single-head fastener 12 is provided at the front end of the connecting seat 11, and the connecting seat 11 is threadedly connected to the mixing shaft 9 through the single-head fastener 12, so as to facilitate the disassembly and replacement of the mixing blades 10. A discharge pipe 4 is provided on one side of the coupling 6, and the discharge pipe 4 and the sealing cover 2 are an integral structure. The inside of the discharge pipe 4 passes through the sealing cover 2, and the material is discharged from the discharge pipe 4. A fixing flange 3 is provided at the connection between the sealing cover 2 and the mixing tank 1, and fixing bolts 19 are provided on the upper ends of the fixing flange 3 to fix the sealing cover 2 and the mixing tank 1. Support legs 8 are provided on the lower surface of the mixing tank 1, and the support legs 8 are welded and fixed to the mixing tank 1. A solenoid valve 20 is provided at the front end of the discharge pipe 7, and the material is discharged from the discharge pipe 7.

[0034] Working principle: When in use, the material to be screened is introduced into the mixing tank 1 from the discharge pipe 4, the mixing shaft 9 is driven to rotate by the stirring motor 5 and the coupling 6, and the mixing blade 10 is used for rapid stirring and mixing, and then precipitation is carried out. The supernatant is taken out to realize the screening of Escherichia coli phage. When stirring and mixing, the mixing blade 10 is used to rotate. When passing through the tough plate 16, the tough plate 16 is bent by force and then rebounds. The rebound improves the mixing effect of the bottom material and the mixing efficiency. At the same time, when the vortex-shaped material splashes upward at the edge, it is limited and blocked by the limit baffle 13, thereby avoiding splashing upward, reducing residue, and saving precipitation time.

[0035] It is obvious to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, the embodiments should be regarded as exemplary and non-restrictive from any point of view, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims be included in the present invention. Any reference numeral in a claim should not be regarded as limiting the claim to which it relates.

Claims

1. An Escherichia coli phage screening device, comprising a mixing tank (1), a sealing cover (2) is arranged above the mixing tank (1), and a discharge pipe (7) is arranged at the lower end of the mixing tank (1); Features: Also includes: A limit baffle (13) is arranged inside the mixing tank (1), a fixing plate (14) is arranged on the upper surface of the limit baffle (13), and four fixing plates (14) are arranged, a fixing screw (15) is arranged on one side of the fixing plate (14), and the fixing plate (14) is threadedly connected to the mixing tank (1) via the fixing screw (15); A tough plate (16) is arranged below the limit baffle (13); a fixed base (17) is arranged at the lower end of the tough plate (16); and four tough plates (16) and four fixed bases (17) are arranged.

2. The E. coli phage screening device according to claim 1, characterized in that: Threaded fasteners (18) are provided on both sides of the fixed base (17), and the fixed base (17) is threadedly connected to the mixing tank (1) via the threaded fasteners (18).

3. The E. coli phage screening device according to claim 1, characterized in that: A coupling (6) is arranged at the upper end of the sealing cover (2), a stirring motor (5) is arranged at the upper end of the coupling (6), and a mixing shaft (9) is arranged at the lower end of the coupling (6).

4. The E. coli phage screening device according to claim 3, characterized in that: A connecting seat (11) is arranged outside the mixing shaft (9), mixing blades (10) are arranged on both sides of the connecting seat (11), a single-head fastener (12) is arranged at the front end of the connecting seat (11), and the connecting seat (11) is threadedly connected to the mixing shaft (9) via the single-head fastener (12).

5. The E. coli phage screening device according to claim 3, characterized in that: A feed pipe (4) is provided on one side of the coupling (6), and the feed pipe (4) and the sealing cover (2) are an integrated structure, and the interior of the feed pipe (4) passes through the sealing cover (2).

6. The E. coli phage screening device according to claim 1, characterized in that: A fixing flange (3) is provided at the connection between the sealing cover (2) and the mixing tank (1), and fixing bolts (19) are provided at the upper ends of the four sides of the fixing flange (3).

7. The E. coli phage screening device according to claim 1, characterized in that: Support legs (8) are provided on the lower surface of the four sides of the mixing tank (1), and the support legs (8) are welded and fixed to the mixing tank (1). A solenoid valve (20) is provided at the front end of the discharge pipe (7).

Images