Cell filter and cell filtering device

Abstract

The utility model provides a cell filter and a cell filtering device, and relates to the field of medical treatment. Wherein the cell filter comprises a filter pipe, a mounting seat, a filter membrane and a pressure release valve, the filter pipe is provided with a mounting position and a liquid discharge hole, the mounting seat is arranged in the mounting position and is in sealing connection with the filter pipe, the mounting seat is provided with a filter hole and a pressure release hole, the filter membrane is laid on the filter hole, the pressure release valve is arranged in the pressure release hole, and the filter hole is communicated with the liquid discharge hole. Or the pressure relief hole is communicated with the liquid discharge hole; the filtering function of the cell filter is achieved by communicating the filtering hole with the liquid discharging hole, when the filtering membrane is not smooth in filtering or blocked, the effect of discharging blocked liquid is achieved by communicating the pressure relief hole with the liquid discharging hole, the situation of not smooth filtering or blocking is avoided, and the success rate of CTCs detection is increased.

Description

Technical Field

[0001] The present application relates to the medical field, and in particular, to a cell filter and a cell filtering device. Background Art

[0002] Circulating tumor cells (CTCs) refer to tumor cells that are released from solid tumors or metastatic lesions into the peripheral blood circulation spontaneously or due to diagnostic and therapeutic procedures. They are an important cause of postoperative recurrence and distant metastasis in patients with malignant tumors.

[0003] In recent years, with the continuous improvement of detection technology, CTCs detection has become a common non-invasive diagnostic tool. Among them, the separation method mainly separates tumor cells based on the size difference between tumor cells and normal cells. During the separation process, the cell filter is prone to filtration obstruction or blockage, and normal cells are not filtered out as required, affecting subsequent staining processing.

[0004] In addition, during the staining process, if the cell filter is blocked or clogged, the unfinished reagents from the previous step will remain in the cell filter. At this time, directly adding the reagents for the next step will directly cause the sample to be scrapped, and the mixing of multiple reagents will produce crystallization, further increasing the degree of blockage of the cell filter and causing failure of CTCs detection. Utility Model Content

[0005] The purpose of the present application is to provide a cell filter and a cell filtration device, which can avoid the occurrence of unsmooth filtration or blockage and improve the success rate of CTCs detection.

[0006] In a first aspect, the utility model provides a cell filter, which comprises a filter tube, a mounting seat, a filter membrane and a pressure relief valve, wherein the filter tube is provided with a mounting position and a drainage hole, the mounting seat is placed in the mounting position and is sealed and connected to the filter tube, and the mounting seat is provided with a filter hole and a pressure relief hole, the filter membrane is laid on the filter hole, the pressure relief valve is installed in the pressure relief hole, the filter hole is connected to the drainage hole, or the pressure relief hole is connected to the drainage hole.

[0007] In an optional embodiment, the filter tube includes a tube body and a drainage part, the side wall of the tube body is provided with the mounting position, the drainage part is threadedly connected to the tube body and is provided with the drainage hole, and when the drainage part is installed in place, the drainage part presses against the mounting seat to make the mounting seat and the tube body sealed and connected.

[0008] In an optional embodiment, the tube body is provided with a containing cavity, the drainage hole is communicated with the containing cavity through the filter hole, or the drainage hole is communicated with the containing cavity through the pressure relief hole.

[0009] In an optional embodiment, the cell filter further comprises a first seal and a second seal, the mounting seat is provided with a first mounting groove and a second mounting groove, the first seal is mounted in the first mounting groove, and the second seal is mounted in the second mounting groove.

[0010] In an optional embodiment, the mounting seat is provided with a first axis, the first axis is perpendicular to the extension direction of the filter hole, and the first mounting groove and the second mounting groove are symmetrically arranged on both sides of the first axis.

[0011] In an optional embodiment, the filter hole and the pressure relief hole are both located within the sealing range of the first sealing component.

[0012] In an optional embodiment, the mounting seat is further provided with an anti-slip convex strip, and the anti-slip convex strip is located outside the mounting position.

[0013] In an optional embodiment, the pressure relief valve includes a third sealing member, a sealing ball, an elastic member and a support member arranged in sequence along the pressure relief direction, and under the action of the elastic member, the sealing ball presses against or separates from the third sealing member.

[0014] In an optional embodiment, the third sealing member is provided with a first pressure relief channel, a second pressure relief channel is provided between the sealing ball and the wall of the pressure relief hole, the elastic member is provided with a third pressure relief channel, and the supporting member is provided with a fourth pressure relief channel, and the first pressure relief channel, the second pressure relief channel, the third pressure relief channel and the fourth pressure relief channel are connected.

[0015] In a second aspect, the utility model provides a cell filtration device, which comprises a waste liquid collector and the cell filter described in the aforementioned embodiment, wherein the cell filter is connected to the waste liquid collector.

[0016] Compared with the prior art, the beneficial effects of this application are:

[0017] The present application realizes the filtering function of the cell filter by connecting the filter hole with the drainage hole. When the filter membrane is blocked or clogged, the present application uses the pressure relief hole to connect with the drainage hole to discharge the blocked liquid, thereby avoiding the occurrence of blocked or clogged filtration and improving the success rate of CTCs detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings required for use in the embodiments will be briefly introduced below. It should be understood that the following drawings only show certain embodiments of the present utility model and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other relevant drawings can be obtained based on these drawings without paying creative work.

[0019] Figure 1 A schematic diagram of the three-dimensional structure of a cell filter in some embodiments is shown;

[0020] Figure 2 An exploded schematic diagram of a cell filter in some embodiments is shown;

[0021] Figure 3 A schematic cross-sectional view of a cell filter in some embodiments is shown;

[0022] Figure 4 A schematic diagram of the three-dimensional structure of the mounting base in some embodiments is shown.

[0023] Description of main component symbols:

[0024] 100-filter tube; 110-tube body; 111-accommodating chamber; 120-drainage portion; 121-drainage hole; 200-mounting seat; 210-filter hole; 220-pressure relief hole; 230-anti-slip convex strip; 300-filter membrane; 400-pressure relief valve; 410-third sealing member; 420-sealing ball; 430-elastic member; 440-support member; 500-first sealing member; 600-second sealing member. DETAILED DESCRIPTION

[0025] The embodiments of the present application are described in detail below, and examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present application, and cannot be understood as limiting the present application.

[0026] In the description of the present application, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present application.

[0027] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of the features. In the description of this application, the meaning of "plurality" is two or more, unless otherwise clearly and specifically defined.

[0028] In this application, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood according to specific circumstances.

[0029] In the present application, unless otherwise clearly specified and limited, a first feature being “above” or “below” a second feature may mean that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, a first feature being “above”, “above”, and “above” a second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is higher in level than the second feature. A first feature being “below”, “below”, and “below” a second feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is lower in level than the second feature.

[0030] Embodiment 1

[0031] Due to individual differences in samples and differences in sample storage time, the samples may be blocked or clogged during cell separation, washing or staining. Therefore, this embodiment provides a cell filter for screening first cells of a first size.

[0032] Exemplarily, in this embodiment, the first cell can be set as a circulating tumor cell. This embodiment is suitable for screening out circulating tumor cells in peripheral blood. The size of circulating tumor cells is larger than that of other normal cells in peripheral blood. This embodiment defines the collected peripheral blood as a sample, and the sample is in liquid form.

[0033] In the separation process, the liquid includes the sample, and in the staining process, the liquid includes the sample and one or more of a plurality of reagents.

[0034] See also Figure 1 and Figure 2 The cell filter includes a filter tube 100 , a mounting seat 200 , a filter membrane 300 and a pressure relief valve 400 .

[0035] See also Figure 2 and Figure 3 The filter tube 100 includes a tube body 110 and a drainage portion 120. The tube body 110 is provided with a accommodating cavity 111. A mounting position is opened on the side wall of the tube body 110. The drainage portion 120 is threadedly connected to the tube body 110 and is provided with a drainage hole 121. The drainage hole 121 is communicated with the accommodating cavity 111. Specifically, an end of the tube body 110 close to the drainage portion 120 is provided with an internal thread, and an end of the drainage portion 120 close to the tube body 110 is provided with an external thread, and the external thread matches the internal thread.

[0036] See also Figure 3 and Figure 4 The mounting seat 200 is placed in the mounting position and sealed with the filter tube 100, and the mounting seat 200 is provided with a filter hole 210 and a pressure relief hole 220. In this embodiment, it can be set that the mounting seat 200 is placed in the mounting position from left to right, and when it is installed in place, the filter hole 210 and the pressure relief hole 220 correspond to the accommodating cavity 111 respectively.

[0037] The drain hole 121 is connected to the accommodating chamber 111 through the filter hole 210 , or the drain hole 121 is connected to the accommodating chamber 111 through the pressure relief hole 220 , that is, the filter hole 210 is connected to the drain hole 121 , or the pressure relief hole 220 is connected to the drain hole 121 .

[0038] Since the filter tube 100 in this embodiment is designed to be a tube body 110 and a drainage portion 120, when the drainage portion 120 is installed in place, the drainage portion 120 presses against the mounting seat 200, so that the mounting seat 200 and the tube body 110 are sealed and fixedly connected. Of course, when the drainage portion 120 and the tube body 110 are separated, the mounting seat 200 loses the force of the drainage portion 120, and the mounting seat 200 can be freely pulled out of the tube body 110.

[0039] To facilitate operation, the mounting seat 200 is also provided with an anti-slip ridge 230. The anti-slip ridge 230 is located outside the mounting position, that is, the anti-slip ridge 230 is exposed relative to the tube body 110. The operator places his hand on the anti-slip ridge 230 and uses the anti-slip ridge 230 as a fulcrum to pull out or push in the mounting seat 200, saving time and effort.

[0040] Please continue reading Figure 2 , Figure 3 and Figure 4The filter membrane 300 is laid on the filter hole 210, that is, the filter membrane 300 is laid flat on the mounting seat 200. When the mounting seat 200 is installed in place, the filter membrane 300 is located at the outlet of the accommodating cavity 111. After a pressure difference is generated above and below the filter membrane 300, the filter membrane 300 can filter the sample, thereby retaining the circulating tumor cells in the sample on the filter membrane 300, and filtering out other normal cells from the drainage hole 121, thereby realizing the filtering function.

[0041] It should be understood that the filter membrane 300 is provided with honeycomb-shaped filter holes 210, the pore size of the filter holes 210 is smaller than the size of circulating tumor cells, and the pore size of the filter holes 210 is larger than the size of normal cells.

[0042] The pressure relief valve 400 is installed in the pressure relief hole 220, and the pressure relief valve 400 has a preset pressure relief pressure. During the separation, flushing or staining process, the sample filtering is not smooth or even blocked, and the pressure in the accommodating chamber 111 is greater than the preset pressure relief pressure. The pressure difference causes the pressure relief valve 400 to open, and other liquid that has not passed through the filter membrane 300 is discharged from the pressure relief valve 400. After all the liquid is discharged, the pressure relief valve 400 is closed. At this time, only the filtered tumor cells or successfully stained tumor cells are retained on the filter membrane 300.

[0043] Specifically, the pressure relief valve 400 includes a third sealing member 410, a sealing ball 420, an elastic member 430 and a support member 440 arranged in sequence along the pressure relief direction. Under the action of the elastic member 430, the sealing ball 420 presses against or separates from the third sealing member 410. In this embodiment, the state in which the sealing ball 420 presses against the third sealing member 410 is defined as the closed state of the pressure relief valve 400, and the state in which the sealing ball 420 separates from the third sealing member 410 is defined as the open state of the pressure relief valve 400.

[0044] During normal filtering or dyeing, the pressure in the accommodating chamber 111 is lower than the preset pressure relief pressure, and the pressure relief valve 400 is in a closed state.

[0045] After filtering is blocked or clogged, the pressure in the accommodating chamber 111 rises, and the pressure in the accommodating chamber 111 is greater than the preset pressure relief pressure, and the pressure relief valve 400 is opened, that is, the pressure above the sealing ball 420 is greater than the pressure below the sealing ball 420. The pressure difference causes the sealing ball 420 to move downward, and the elastic member 430 is compressed until the pressure difference is restored, the elastic member 430 restores its deformation, and the sealing ball 420 moves upward under the action of the elastic member 430, and the pressure relief valve 400 is closed again.

[0046] In this embodiment, the elastic member 430 is configured as a tension spring, and the purpose of making the pressure relief valve 400 have a preset pressure relief pressure is achieved by setting the elastic coefficient of the elastic member 430 .

[0047] The third sealing member 410 is provided with a first pressure relief channel, a second pressure relief channel is provided between the sealing ball 420 and the wall of the pressure relief hole 220, the elastic member 430 is provided with a third pressure relief channel, and the supporting member 440 is provided with a fourth pressure relief channel. The first pressure relief channel, the second pressure relief channel, the third pressure relief channel and the fourth pressure relief channel are connected.

[0048] In this embodiment, the pressure relief valve 400 is provided to discharge the blocked liquid, thereby avoiding the situation of unsmooth filtering or blockage, and improving the success rate of CTCs detection.

[0049] The sealing connection method of this embodiment is as follows:

[0050] The cell filter further comprises a first sealing member 500 and a second sealing member 600. The mounting seat 200 is provided with a first mounting groove and a second mounting groove. The first sealing member 500 is mounted in the first mounting groove, and the second sealing member 600 is mounted in the second mounting groove.

[0051] The mounting seat 200 is provided with a first axis, which is perpendicular to the extension direction of the filter hole 210. The first mounting groove and the second mounting groove are symmetrically arranged on both sides of the first axis. In this embodiment, it can be set that the first mounting groove and the second mounting groove are coaxial, and the first mounting groove and the second mounting groove have the same shape.

[0052] It is worth noting that the filter hole 210 and the pressure relief hole 220 are both located within the sealing range of the first seal 500, and the filter hole 210 and the pressure relief hole 220 are also located within the sealing range of the second seal 600, thereby ensuring that the liquid will not overflow when flowing from the filter hole 210 or the pressure relief hole 220. On the one hand, it prevents the external environment from affecting the sample, and on the other hand, the sample will not overflow into the outside world and pollute the environment.

[0053] Based on the above content, this embodiment further provides a cell filtering device, which includes a waste liquid collector and the above-mentioned cell filter, and the cell filter is connected to the waste liquid collector.

[0054] The waste liquid collector has a preset negative pressure. When the waste liquid collector is connected to the cell filter, a pressure difference is generated above and below the filter membrane 300 to achieve filtration.

[0055] The waste liquid collector may be a blood collection tube or other container with a preset negative pressure state.

[0056] Based on the above content, the filtering principle of the cell filtration device is further described in this embodiment as follows:

[0057] S100. The filter membrane 300, the pressure relief valve 400, the first seal 500 and the second seal 600 are respectively installed on the mounting seat 200;

[0058] S200. Push the mounting base 200 from left to right into the mounting position of the tube body 110;

[0059] S300. The drain portion 120 is threadedly connected to the tube body 110. After being installed in place, the drain portion 120 abuts against the mounting seat 200, so that the mounting seat 200 is fixed on the tube body 110;

[0060] S400. Connecting the waste liquid collector to the drain portion 120;

[0061] S500. The negative pressure attached to the waste liquid collector causes normal cells to be discharged from the drainage hole 121 through the filter membrane 300. When the filter membrane 300 is blocked or blocked, the pressure relief valve 400 opens under the action of the pressure difference, and the normal cells and the remaining tumor cells are all discharged from the pressure relief valve 400, and the filtered tumor cells are retained on the filter membrane 300;

[0062] S600. After the pressure difference disappears, the pressure relief valve 400 is closed.

[0063] S700 . Release the connection between the waste liquid collector and the drain portion 120 , unscrew the drain portion 120 , and pull the mounting seat 200 out of the tube body 110 .

[0064] It is understandable that in S600, the sample can continue to be stained as required. If the filter membrane 300 is blocked during the staining process, steps S500 and S600 are repeated. This embodiment can ensure that the sample will not be scrapped due to blockage of the filter membrane 300.

[0065] In order to further enhance the monitoring function of this embodiment, a pressure gauge is provided on the cell filtration device to perform real-time online monitoring of the pressure in the cell filtration device. If the filter membrane 300 is clogged and the pressure reaches a preset value, the pressure relief valve 400 can be quickly opened to release the pressure. The pressure gauge records this and sends the record to the controller for judgment, thereby prompting the user that the sample has a blockage abnormality, which plays a role in assisting the judgment of the test data.

[0066] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present application. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, without contradiction.

[0067] Although the embodiments of the present application have been shown and described above, it can be understood that the above embodiments are exemplary and cannot be understood as limitations on the present application. Ordinary technicians in this field can change, modify, replace and modify the above embodiments within the scope of the present application.

Claims

1. A cell filter, characterized in that It includes a filter tube, a mounting seat, a filter membrane and a pressure relief valve, the filter tube is provided with a mounting position and a drainage hole, the mounting seat is placed in the mounting position and is sealed with the filter tube, and the mounting seat is provided with a filter hole and a pressure relief hole, the filter membrane is laid on the filter hole, the pressure relief valve is installed in the pressure relief hole, the filter hole is connected with the drainage hole, or the pressure relief hole is connected with the drainage hole.

2. The cell filter according to claim 1, characterized in that The filter tube includes a tube body and a drainage part. The side wall of the tube body is provided with the installation position. The drainage part is threadedly connected to the tube body and is provided with the drainage hole. When the drainage part is installed in place, the drainage part presses against the mounting seat to make the mounting seat and the tube body sealed and connected.

3. The cell filter according to claim 2, characterized in that The tube body is provided with a containing cavity, the drainage hole is communicated with the containing cavity through the filtering hole, or the drainage hole is communicated with the containing cavity through the pressure relief hole.

4. The cell filter according to any one of claims 1 to 3, characterized in that It also includes a first sealing member and a second sealing member. The mounting seat is provided with a first mounting groove and a second mounting groove. The first sealing member is mounted in the first mounting groove, and the second sealing member is mounted in the second mounting groove.

5. The cell filter according to claim 4, characterized in that The mounting seat is provided with a first axis, the first axis is perpendicular to the extension direction of the filter hole, and the first mounting groove and the second mounting groove are symmetrically arranged on both sides of the first axis.

6. The cell filter according to claim 5, characterized in that The filter hole and the pressure relief hole are both located within the sealing range of the first sealing component.

7. The cell filter according to any one of claims 1 to 3, characterized in that The mounting seat is also provided with an anti-skid convex strip, and the anti-skid convex strip is located outside the mounting position.

8. The cell filter according to any one of claims 1 to 3, characterized in that The pressure relief valve comprises a third sealing member, a sealing ball, an elastic member and a supporting member which are sequentially arranged along the pressure relief direction. Under the action of the elastic member, the sealing ball presses against or separates from the third sealing member.

9. The cell filter according to claim 8, characterized in that The third sealing component is provided with a first pressure relief channel, a second pressure relief channel is provided between the sealing ball and the wall of the pressure relief hole, the elastic component is provided with a third pressure relief channel, and the supporting component is provided with a fourth pressure relief channel. The first pressure relief channel, the second pressure relief channel, the third pressure relief channel and the fourth pressure relief channel are connected.

10. A cell filtration device, characterized in that: The invention comprises a waste liquid collector and the cell filter according to any one of claims 1 to 9, wherein the cell filter is connected to the waste liquid collector.

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