Nucleic acid detection device

Abstract

The utility model provides a nucleic acid detection device and belongs to the technical field of nucleic acid detection. The nucleic acid detection device comprises a body and a heating cylinder, three partition plates are installed in an inner cavity of the heating cylinder, a circulation mechanism comprises a circulation cover, a circulation box, a containing cavity and a sliding block, the circulation cover is rotatably installed at the top of the heating cylinder, the circulation box is fixedly installed at the top of the circulation cover, and the containing cavity is fixedly installed at the bottom of the circulation cover. And the accommodating cavity is formed between the circulating box and the circulating cover. The circulating mechanism is arranged, the motor is started, the reciprocating lead screw is driven to rotate through the first follow-up rod, the sliding block drives the test tube to move downwards, meanwhile, the second follow-up rod drives the gear to rotate, the gear drives the circulating cover to rotate until the test tube reaches a preset position and enters the heating cavity for denaturation, and after denaturation is completed, the motor is started again, and the test tube is heated. And the test tube enters the heating cavity for annealing, and the operation is repeated, so that the problem that the test tube needs to be transferred manually is solved.

Description

Technical Field

[0001] The utility model relates to the technical field of nucleic acid detection, and in particular to a nucleic acid detection device. Background Art

[0002] Nucleic acid testing is a method to determine whether a person is infected with a virus by detecting the genetic material of the virus. It collects samples such as throat swabs and nasopharyngeal swabs and uses fluorescent quantitative PCR technology to detect the presence of viral RNA. In PCR technology, thermal cycling is required to amplify DNA. This process mainly includes denaturation, annealing and extension.

[0003] In the prior art, when performing thermal cycling operations, test tubes need to be manually transferred and processed in heating chambers of different temperatures in turn, which is very cumbersome and the effect is greatly affected by manual factors. In addition, when the test tubes are transferred, the temperature of the test tubes should be kept within a certain range to ensure the accuracy of DNA replication. Utility Model Content

[0004] In order to make up for the above deficiencies, the utility model provides a nucleic acid detection device that overcomes the above technical problems or at least partially solves the above problems.

[0005] The utility model is achieved in this way:

[0006] The utility model provides a nucleic acid detection device, comprising a body and a heating cylinder, wherein three partitions are installed in the inner cavity of the heating cylinder.

[0007] A circulation mechanism, the circulation mechanism comprising:

[0008] A circulation cover, which is rotatably mounted on the top of the heating cylinder;

[0009] A circulation box, wherein the circulation box is fixedly mounted on the top of the circulation cover;

[0010] An accommodating chamber, wherein the accommodating chamber is provided between the circulation box and the circulation cover;

[0011] A slider, the slider being slidably mounted inside the accommodating cavity;

[0012] A temperature insulation mechanism is arranged inside the circulation box.

[0013] In a preferred embodiment, the heat preservation mechanism comprises:

[0014] A fixing block, the fixing block being fixedly mounted on the left side of the inner wall of the accommodating cavity;

[0015] A movable groove, the movable groove is opened on the right side of the accommodating cavity;

[0016] The insulation block is slidably mounted inside the movable groove, and the left side of the insulation block penetrates into the interior of the fixed block.

[0017] In a preferred solution, a reciprocating screw is rotatably mounted on the top of the fixed block, the reciprocating screw is sleeved inside the slider and threadedly connected to the slider, and a first follower rod is fixedly mounted on the top of the reciprocating screw.

[0018] In a preferred solution, a second follower rod is rotatably mounted inside the circulation box, surfaces of the first follower rod and the second follower rod are both sleeved with rotating wheels, and a transmission belt is sleeved between the first follower rod and the second follower rod via the rotating wheel.

[0019] In a preferred solution, a gear is fixedly mounted on the bottom of the second follower rod, and meshing teeth are fixedly mounted on the inner wall of the heating tube, and the meshing teeth are meshingly connected with the gear.

[0020] In a preferred solution, a spring and a telescopic rod are fixedly installed on the right side of the inner wall of the movable groove, and the left side of the spring and the telescopic rod is fixedly connected to the right side of the insulation block.

[0021] In a preferred solution, a hollow column is installed between the three partitions, three heaters are installed inside the hollow column, and the shape of the top of the insulation block is set to be arc-shaped.

[0022] In a preferred solution, a motor is fixedly mounted on the top of the circulation box, and an output end of the motor is fixedly connected to the top of the first follower rod.

[0023] The utility model provides a nucleic acid detection device, the beneficial effects of which include:

[0024] 1. By setting up a circulation mechanism, starting the motor, and driving the reciprocating screw to rotate through the first follower rod, the slider drives the test tube to move downward, and at the same time, the second follower rod drives the gear to rotate, and the gear drives the circulation cover to rotate until it reaches the preset position, and the test tube enters the heating chamber for denaturation. After the denaturation is completed, the motor is started again to make the test tube enter the heating chamber for annealing, and this process is repeated, thereby solving the problem of manual transfer of the test tube.

[0025] 2. By setting up a heat preservation mechanism, when the test tube moves downward, it is squeezed by the test tube and moves to the right to the inside of the moving groove. When the test tube moves upward and no longer squeezes the insulation block, the insulation block pops out through the resilience of the spring and penetrates into the inside of the fixed block, sealing the internal space of the accommodating cavity to prevent the test tube from suddenly cooling down during the transfer process, thereby avoiding the sudden cooling of the test tube from affecting the accuracy of the test result. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] In order to more clearly illustrate the technical solution of the implementation mode of the utility model, the drawings required for use in the implementation mode will be briefly introduced below. It should be understood that the following drawings only show certain embodiments of the utility model and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other relevant drawings can be obtained based on these drawings without paying creative work.

[0027] Figure 1 It is a schematic diagram of the overall structure provided by the implementation method of the utility model;

[0028] Figure 2 A partial cross-sectional view of a heating cylinder is provided for an embodiment of the utility model;

[0029] Figure 3 A partial cross-sectional view of a circulation box and a circulation cover is provided for an embodiment of the utility model;

[0030] Figure 4 Provided for the implementation of the utility model Figure 2 A partial enlarged view of the middle A;

[0031] Figure 5 Provided for the implementation of the utility model Figure 3 A partial enlarged view of point B in the middle.

[0032] In the figure: 1. main body; 2. heating cylinder; 3. partition; 4. circulation cover; 5. circulation box; 6. accommodating chamber; 7. slider; 8. fixed block; 9. moving groove; 10. insulation block; 11. reciprocating screw rod; 12. first follower rod; 13. second follower rod; 14. transmission belt; 15. gear; 16. meshing teeth; 17. spring; 18. telescopic rod; 19. hollow column; 20. heater; 21. motor. DETAILED DESCRIPTION

[0033] In order to make the purpose, technical solution and advantages of the implementation of the utility model clearer, the technical solution in the implementation of the utility model will be clearly and completely described below in conjunction with the drawings in the implementation of the utility model. Obviously, the described implementation is a part of the implementation of the utility model, not all of the implementations. Based on the implementation of the utility model, all other implementations obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0034] Reference Figure 1-5The utility model provides a technical solution: a nucleic acid detection device, including a body 1 and a heating cylinder 2, three partitions 3 are installed in the inner cavity of the heating cylinder 2, the circulation mechanism includes a circulation cover 4, a circulation box 5, a accommodating chamber 6 and a slider 7, the circulation cover 4 is rotatably installed on the top of the heating cylinder 2, the circulation box 5 is fixedly installed on the top of the circulation cover 4, the accommodating chamber 6 is opened between the circulation box 5 and the circulation cover 4, the slider 7 is slidably installed in the accommodating chamber 6, the temperature insulation mechanism is arranged in the inside of the circulation box 5, the top of the fixed block 8 is rotatably installed with a reciprocating screw rod 11, the reciprocating screw rod 11 is sleeved in the inside of the slider 7, and is threadedly connected to the slider 7, and the reciprocating screw rod 11 is rotatably installed in the top of the fixed block 8. A first follower rod 12 is fixedly installed on the top of the multifilament rod 11, and a second follower rod 13 is rotatably installed inside the circulation box 5. The surfaces of the first follower rod 12 and the second follower rod 13 are sleeved with a rotating wheel, and a transmission belt 14 is sleeved between the first follower rod 12 and the second follower rod 13 through the rotating wheel. A gear 15 is fixedly installed on the bottom of the second follower rod 13, and a meshing tooth 16 is fixedly installed on the inner wall of the heating cylinder 2. The meshing tooth 16 and the gear 15 are meshed and connected. A motor 21 is fixedly installed on the top of the circulation box 5, and the output end of the motor 21 is fixedly connected to the top of the first follower rod 12. By setting a circulation mechanism, the three partitions 3 will heat The inner cavity of the cylinder 2 is divided into three independent spaces. The heater 20 is started to make the temperatures of the three independent spaces reach the temperatures required for the three steps of the thermal cycle respectively. The box door on the circulation box 5 is opened, and the test tube is clamped by the clamping device installed on the slider 7. This is a prior art and will not be described in detail. The box door is closed, and the motor 21 is started. The reciprocating screw 11 is driven to rotate by the first follower rod 12. Since the shape of the slider 7 is consistent with the shape of the accommodating chamber 6, the slider 7 drives the test tube to move downward. Through the cooperation of the transmission belt 14, the second follower rod 13 drives the gear 15 to rotate at the same time. The gear 15 is meshed with the meshing tooth 16 to make the gear 15 rotate. The wheel 15 drives the circulation cover 4 to rotate until it reaches the preset position, and the test tube enters the heating chamber for denaturation. After the denaturation is completed, the motor 21 is started again, so that the slider 7 drives the test tube to move upward to the inside of the accommodating chamber 6, and then extends downward from the inside of the accommodating chamber 6. In this process, the circulation cover 4 drives the circulation box 5 to move to the top of the heating chamber for annealing through the cooperation of the gear 15, so that the test tube enters the heating chamber for annealing, and the above steps are repeated to put the test tube into the heating chamber for extension, and this is repeated until the test sample meets the requirements, thereby solving the problem of the prior art that the test tube needs to be manually transferred;

[0035] It is worth mentioning that the temperature required for denaturation is usually 94-98°C, the temperature required for annealing is usually 50-65°C, and the temperature required for extension is usually 72°C. One thermal cycle consists of denaturation, annealing, and extension. Usually, 25-35 thermal cycles are required to meet the requirements.

[0036] Reference Figure 1-5The heat preservation mechanism includes a fixed block 8, a movable groove 9 and an insulation block 10. The fixed block 8 is fixedly installed on the left side of the inner wall of the accommodating chamber 6. The movable groove 9 is opened on the right side of the accommodating chamber 6. The insulation block 10 is slidably installed inside the movable groove 9. The left side of the insulation block 10 penetrates into the interior of the fixed block 8. A spring 17 and a telescopic rod 18 are fixedly installed on the right side of the inner wall of the movable groove 9. The left side of the spring 17 and the telescopic rod 18 are fixedly connected to the right side of the insulation block 10. A hollow column 19 is installed between the three partitions 3, and three heaters 20 are installed inside the hollow column 19. The shape of the top of the insulation block 10 is set to be an arc. By setting up a heat preservation mechanism, when the test tube moves downward, due to the shape of the top of the insulation block 10 being set to be an arc, it is squeezed by the test tube and moves to the right to the inside of the moving groove 9. When the test tube moves upward and no longer squeezes the insulation block 10, the insulation block 10 is ejected by the rebound force of the spring 17 and penetrates into the inside of the fixed block 8, closing the internal space of the accommodating chamber 6 to prevent the test tube from suddenly cooling during the transfer process, thereby avoiding the sudden cooling of the test tube from affecting the accuracy of the test result.

[0037] Specifically, the working process or working principle of the nucleic acid detection device is as follows: when in use, the three partitions 3 divide the inner cavity of the heating tube 2 into three independent spaces, and the heater 20 is started to make the temperatures of the three independent spaces reach the temperatures required for the three steps of the thermal cycle respectively. The box door on the circulation box 5 is opened, and the test tube is clamped by the clamping device installed on the slider 7. This is a prior art and will not be described in detail. The box door is closed, and the motor 21 is started. The reciprocating screw rod 11 is driven to rotate by the first follower rod 12. Since the shape of the slider 7 is consistent with the shape of the accommodating cavity 6, the slider 7 drives the test tube to move downward. Through the cooperation of the transmission belt 14, the second follower rod 13 drives the gear 15 to rotate at the same time. Through the meshing connection between the gear 15 and the meshing tooth 16, the gear 15 drives the circulation cover 4 to rotate. After reaching the preset position, the test tube enters the heating cavity for denaturation. After the denaturation is completed, the motor 21 is started again, so that the slider 7 drives the test tube to move upward to the inside of the accommodating chamber 6, and then extends downward from the inside of the accommodating chamber 6. In this process, through the cooperation of the gear 15, the circulation cover 4 drives the circulation box 5 to move above the heating chamber for annealing, so that the test tube enters the heating chamber for annealing, and repeats the above steps to put the test tube into the heating chamber for extension, and repeats this process until the test sample meets the requirements. When the test tube moves downward, since the shape of the top of the insulation block 10 is set to be arc-shaped, it is squeezed by the test tube and moves to the right to the inside of the moving groove 9. When the test tube moves upward and no longer squeezes the insulation block 10, the insulation block 10 is ejected by the resilience of the spring 17 and penetrates into the inside of the fixed block 8, The internal space of the accommodating chamber 6 is closed to prevent the test tube from suddenly cooling during the transfer process.

[0038] It should be noted that the heater 20 and the motor 21 are devices or equipment existing in the prior art, or are devices or equipment that can be realized in the prior art. Their power supply, specific composition and principles are clear to those skilled in the art, so they are not described in detail.

Claims

1. A nucleic acid detection device, comprising a body (1) and a heating cylinder (2), wherein three partitions (3) are installed in the inner cavity of the heating cylinder (2), characterized in that: A circulation mechanism, the circulation mechanism comprising: A circulation cover (4), wherein the circulation cover (4) is rotatably mounted on the top of the heating cylinder (2); A circulation box (5), wherein the circulation box (5) is fixedly mounted on the top of the circulation cover (4); A containing chamber (6), wherein the containing chamber (6) is provided between the circulation box (5) and the circulation cover (4); A slider (7), wherein the slider (7) is slidably mounted inside the accommodating cavity (6); A temperature insulation mechanism is arranged inside the circulation box (5).

2. A nucleic acid detection device according to claim 1, characterized in that: The temperature insulation mechanism comprises: A fixing block (8), wherein the fixing block (8) is fixedly mounted on the left side of the inner wall of the accommodating chamber (6); A movable groove (9), wherein the movable groove (9) is disposed on the right side of the accommodating cavity (6); A temperature insulation block (10), wherein the temperature insulation block (10) is slidably mounted inside the movable groove (9), and the left side of the temperature insulation block (10) penetrates into the interior of the fixed block (8).

3. A nucleic acid detection device according to claim 2, characterized in that: A reciprocating screw rod (11) is rotatably mounted on the top of the fixed block (8); the reciprocating screw rod (11) is sleeved inside the slider (7) and is threadedly connected to the slider (7); a first follower rod (12) is fixedly mounted on the top of the reciprocating screw rod (11).

4. A nucleic acid detection device according to claim 3, characterized in that: A second follower rod (13) is rotatably mounted inside the circulation box (5); rotating wheels are sleeved on the surfaces of the first follower rod (12) and the second follower rod (13); and a transmission belt (14) is sleeved between the first follower rod (12) and the second follower rod (13) via the rotating wheels.

5. A nucleic acid detection device according to claim 4, characterized in that: A gear (15) is fixedly mounted on the bottom of the second follower rod (13), and a meshing tooth (16) is fixedly mounted on the inner wall of the heating cylinder (2), wherein the meshing tooth (16) is meshingly connected with the gear (15).

6. A nucleic acid detection device according to claim 2, characterized in that: A spring (17) and a telescopic rod (18) are fixedly installed on the right side of the inner wall of the movable groove (9), and the left sides of the spring (17) and the telescopic rod (18) are fixedly connected to the right side of the thermal insulation block (10).

7. A nucleic acid detection device according to claim 6, characterized in that: A hollow column (19) is installed between the three partitions (3), three heaters (20) are installed inside the hollow column (19), and the shape of the top of the temperature insulation block (10) is set to be arc-shaped.

8. A nucleic acid detection device according to claim 4, characterized in that: A motor (21) is fixedly mounted on the top of the circulation box (5), and an output end of the motor (21) is fixedly connected to the top of the first follower rod (12).

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