Lactobacillus oral granules for improving athletic ability

Abstract

The utility model provides a lactobacillus oral particle for improving athletic ability, which comprises a plurality of lactobacillus particles and an environment isolation layer, the space between the lactobacillus particles and the environment isolation layer is filled with a muscle resistance increasing layer, and the outer side of the environment isolation layer is sequentially provided with a peculiar smell prevention layer and a flow aiding layer; according to the application, the arrangement of the muscle tolerance layer is also a nutritional organic substance for the lactobacillus granules, so that the lactobacillus granules can be promoted to maintain proliferation activity after being stored for a long time, and the muscle tolerance of a host can be enhanced after the lactobacillus granules are eaten; and the flow aiding layer is arranged, so that the flowability of the particle structure in the later filling process can be improved when the particle structure is prepared into various dosage forms, the particle structure is simple to process and easy to form, and the production efficiency is improved.

Description

Technical Field

[0001] The present application relates to the field of oral granules, and in particular to a Lactobacillus oral granule for improving motility. Background Art

[0002] Exercise capacity (EC) refers to the ability of a person to participate in sports and training. It is a comprehensive expression of factors such as a person's physical shape, quality, function, skills and psychological ability. From a biochemical point of view, the level of exercise capacity mainly depends on the ability to supply, transfer and utilize energy during exercise. Existing technologies improve exercise capacity through the following ways:

[0003] 1. Adjust body shape: patients need to do morning jogging, maintain a healthy and positive mentality, etc. 2. Improve personal cultural quality: patients need to understand cultural knowledge, sports knowledge, competition rules, etc., can enrich reading and broaden personal horizons. 3. Adjust human body functions: patients usually need to exercise their physical abilities and do more exercise. And ensure a regular daily routine, such as going to bed early, getting up early, and not staying up late. 4. Enhance human nutritional supplements: a balanced combination of meat and vegetables is required to maintain personal nutritional balance and normal appetite.

[0004] Recent studies have found that lactobacillus has the ability to enhance athletic ability. Therefore, for the sake of convenience, more and more people take lactobacillus orally to enhance their athletic ability. In order to maintain the high activity and stability of lactobacillus, a layered particle structure is used in the prior art.

[0005] For example, the prior art application number of "Oral microparticles for maintaining high activity and stability of lactic acid bacteria" is 2017211301162, which includes a lactic acid bacteria particle, an anti-dehydration layer coated on the outside of the lactic acid bacteria particle, an anti-damage layer coated on the outside of the anti-dehydration layer, an antioxidant layer coated on the outside of the anti-damage layer, an oligosaccharide layer coated on the outside of the antioxidant layer, and a polysaccharide layer coated on the outside of the oligosaccharide layer. This application cannot enhance the host's muscle endurance after consumption, and cannot ensure the fluidity of the later filling process when the microparticle structure is made into various dosage forms.

[0006] Therefore, it is necessary to provide a new technical solution to solve the above technical problems. Utility Model Content

[0007] The present application provides an oral lactobacillus granule for improving athletic ability, comprising a plurality of lactobacillus granules and an environmental isolation layer, a muscle endurance increasing layer is filled between the lactobacillus granules and the environmental isolation layer, and an odor prevention layer and a flow-aiding layer are sequentially arranged on the outside of the environmental isolation layer.

[0008] As a preferred embodiment, the method for increasing the muscle resistance layer adopts any one of arginine, whey protein, L-carnitine, creatine, vitamin D, vitamin E, vitamin B group, trace minerals, and comprehensive amino acids.

[0009] As a preferred solution, the insulating environment layer is made of any one of glycerol, chicory fiber, resistant dextrin, oat fiber, oligosaccharides, microcrystalline cellulose, corn starch, lecithin and trehalose.

[0010] As a preferred embodiment, the odor barrier layer is made of any one of diglyceride, monoglyceride, green tea powder, monk fruit extract, yeast extract, tea extract, orange peel extract and lemon peel extract.

[0011] As a preferred embodiment, the flow-aiding layer is made of any one of magnesium stearate, silicon dioxide, calcium hydrogen phosphate, microcrystalline cellulose, micro-powder silica gel, lactose, fatty acid glycosides and fatty acid sucrose glycosides.

[0012] In the present application, the setting of adding the muscle resistance layer is also a nutrient for the lactobacillus particles, so it can not only promote the lactobacillus particles to maintain their proliferation activity under long-term storage, but also enhance the host's muscle endurance after consumption; and the setting of the flow-aiding layer can improve the fluidity of the subsequent filling process when the microparticle structure is made into various dosage forms, making the present application simple to process and easy to shape, thereby improving production efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 It is a schematic diagram of the structure of this application;

[0014] Figure 2 is the experimental flow chart of this application;

[0015] Figure 3 It is a comparative diagram of the effect of feeding sterile water and the oral granules of the present application on running ability;

[0016] Figure 4 It is a comparison chart of the effects of tube-fed sterile water and the oral granules of the present application on mtDNA in the gastrocnemius muscle of mice;

[0017] Figure 5 It is a comparative diagram of the effects of sterile water and the oral granules of the present application on the ROS gene in the gastrocnemius muscle of mice;

[0018] 110. Lactobacillus particles; 120. Muscle resistance increasing layer; 130. Environment isolation layer; 140. Odor prevention layer; 150. Flow-aiding layer. DETAILED DESCRIPTION

[0019] The specific implementation of the present invention is described in detail below in conjunction with the accompanying drawings. It should be noted that the specific implementation described here is only used to illustrate and explain the present invention, and is not used to limit the present invention.

[0020] The present application provides an oral lactobacillus granule for improving athletic ability, comprising a plurality of lactobacillus granules 110, wherein the lactobacillus granules 110 are obtained by centrifugation and concentration after fermentation and cultivation of lactobacillus with various functions; the outer side of the lactobacillus granules 110 is coated with a muscle endurance increasing layer 120, which is a nutrient substance for the lactobacillus granules, and thus is arranged on the outer side of the lactobacillus granules 110 to promote the lactobacillus granules 110 to maintain proliferation activity under long-term storage, and can also enhance the host's muscle endurance after consumption; the muscle endurance increasing layer 120 uses any one of arginine, whey protein, L-carnitine, creatine, vitamin D, vitamin E, vitamin B group, trace minerals, and comprehensive amino acids.

[0021] The outer side of the muscle resistance increasing layer 120 is coated with an environment isolation layer 130, such as Figure 1 As shown, a muscle-increasing layer 120 is also provided between the muscle-increasing layer 120 on the outside of the lactobacillus particle 110, that is, the muscle-increasing layer 120 is filled between the lactobacillus particle 110 and the environmental isolation layer 130. The setting of the environmental isolation layer 130 can ensure that the lactobacillus particle 110 is not persecuted by the temperature, pressure, and oxidation in the environment, thereby achieving the purpose of improving the survival rate of the lactobacillus particle 110 in the particle structure. The environmental isolation layer 130 adopts any one of glycerol, chicory fiber, resistant dextrin, oat fiber, oligosaccharide, microcrystalline cellulose, corn starch, lecithin and trehalose.

[0022] The outer side of the isolation layer 130 is coated with an odor barrier layer 140 to prevent the lactobacillus particles 110 from damaging the flavor of the product due to the unpalatable flavor of the fermentation medium matrix, thereby achieving the purpose of increasing customers' preference for consumption.

[0023] A flow-aiding layer 150 is disposed outside the odor barrier layer 140, and its purpose is to improve the fluidity of the subsequent filling process when the particulate structure is made into various dosage forms; the flow-aiding layer 150 is made of any one of magnesium stearate, silicon dioxide, calcium hydrogen phosphate, microcrystalline cellulose, micropowder silica gel, lactose, fatty acid glycosides and fatty acid sucrose esters.

[0024] The present application provides the following experimental methods and experimental results of lactobacillus being able to enhance exercise capacity:

[0025] 1. Experimental purpose: Oral administration of Lactobacillus granules can help improve the motor ability of mice;

[0026] 2. Experimental Methods: 20-month-old C57BL / 6 female mice (equivalent to 60 years old in humans) were gavaged with sterile water (Ctrl) (number of mice = 5) or Lactobacillus oral microparticles (dose: 1x10 9 cfu / mice / day) (number = 5), the process is as follows Figure 2 As shown:

[0027] 3. Steps of running experiment

[0028] 1. Pre-experimental training

[0029] (1) Place each batch of mice (5 mice) on a roller treadmill (Ugo Basile Rota-Rod 47600) and let it stand for a few minutes;

[0030] (2) Clean the machine with alcohol between each batch to remove feces, urine and odor;

[0031] (3) The first group of mice were placed on a roller treadmill at a fixed speed of 4 rpm for 1 minute;

[0032] (4) Clean the machine with alcohol to remove mouse feces, urine, and odor before proceeding to the next batch;

[0033] (5) Continue to practice running for one week before starting the formal experiment.

[0034] 2. Training before the formal experiment

[0035] (1) Place the mouse on a roller treadmill at a fixed speed of 4 rpm and run for 1 minute;

[0036] (2) Clean the machine with alcohol between each batch to remove feces, urine and odor;

[0037] (3) Each mouse was allowed to rest for at least 10 minutes before entering the formal experiment;

[0038] (III) Formal experimental process:

[0039] (1) After setting the parameters of the roller treadmill, start rotating at 4 rpm;

[0040] (2) The first group of mice were placed on the roller treadmill and the start button was pressed. The treadmill speed was accelerated from 4 rpm to 30 rpm within 5 minutes, and then kept at 30 rpm for another 5 minutes.

[0041] (3) Record the time it takes for each mouse to fall off the wheel;

[0042] (4) After the 10-minute running time, the mice were returned to the cage and allowed to rest for at least 10 minutes;

[0043] (5) Clean the machine with alcohol to remove mouse feces, urine, and odor before proceeding to the next batch;

[0044] (6) The next batch of mice repeats the above steps until all mice have completed one run, which is called a trial.

[0045] (7) Each mouse ran three times a day, and the average time of each mouse running on the wheel for three rounds was calculated for statistical analysis;

[0046] (8) The time points for the running experiment were before the experiment (start), the first month of the experiment (four weeks after feeding), the second month of the experiment, and the third month of the experiment (end point);

[0047] (9) If the mouse falls off the treadmill within 10 seconds after the start of the timing, the mouse must be caught back on the wheel bar and the experiment must be continued. The two running times of each mouse must be at least 10 minutes apart to allow the mouse to have sufficient rest. In the experimental statistical analysis, the longer the running time on the wheel, the better the athletic performance.

[0048] IV. Experimental steps for muscle-related gene expression:

[0049] 1. Experimental end point (12th week) 2 After sacrifice, the fur on the outside of the legs was removed to expose the muscle part, and the GA (gastrocnemius) of the left and right feet of the mouse was removed in sequence and stored at -80°C for RNA extraction.

[0050] 2. Steps for extracting muscle RNA:

[0051] (1) Add 0.5 ml TRIzol TM Reagent to (320103, EOG sterilized) in a 1.5 ml microtube (with a rough grinding surface), and grind with a dedicated electric mixer (PowerMasher II, nippi) until the whole muscle is homogenized;

[0052] (2) RNA extraction steps are as follows: add 0.1 ml chloroform, invert 15 times, react at room temperature for 5 min, and then centrifuge at 12,000 g for 15 min; extract the upper layer into a new eppendorf, add 0.25 ml isopropanol, and precipitate at -80°C overnight; the next day, centrifuge for 10 minutes, remove the supernatant, and wash the RNA precipitate with 0.5 ml 75% alcohol; dissolve the RNA in DEPC water, and measure the RNA concentration;

[0053] (3) Equal amounts of RNA were used to reverse cDNA and muscle cDNA was subjected to real-time PCR analysis: the cDNA was placed in a Q-PCR machine (model QIAGEN: Rotor-Gene Q 2Plex) for reaction. The following related genes were expressed as relative amounts after subtracting their own ACTB or GAPDH (2 -△Ct ).

[0054] Through the above experimental steps and results, it can be clearly seen that Lactobacillus has a significant effect on improving athletic ability.

[0055] In the present application, the setting of adding the muscle resistance layer 120 is also a nutrient for the lactobacillus particles, so it can not only promote the lactobacillus particles to maintain their proliferation activity under long-term storage, but also enhance the host's muscle endurance after consumption; and the setting of the flow-aiding layer can improve the fluidity of the subsequent filling process when the microparticle structure is made into various dosage forms, making the present application simple to process and easy to shape, thereby improving production efficiency.

[0056] The present invention has been described through the above-mentioned relevant embodiments, however, the above-mentioned embodiments are only examples for implementing the present invention; it must be pointed out that the disclosed embodiments do not limit the scope of the present invention. On the contrary, modifications and equivalent settings contained in the spirit and scope of the claims are all included in the scope of the present invention.

Claims

1. A lactobacillus oral granule for improving motility, comprising a plurality of lactobacillus granules (110) and an environmental isolation layer (130), characterized in that: A muscle resistance increasing layer (120) is filled between the lactobacillus particles (110) and the environmental isolation layer (130), and an odor prevention layer (140) and a flow-aiding layer (150) are sequentially arranged on the outer side of the environmental isolation layer (130).

2. The Lactobacillus oral granules for improving exercise ability according to claim 1, characterized in that: The muscle resistance layer (120) is increased by using any one of arginine, whey protein, L-carnitine, creatine, vitamin D, vitamin E, vitamin B group, trace minerals, and comprehensive amino acids.

3. The Lactobacillus oral granules for improving exercise ability according to claim 1, characterized in that: The insulating environment layer (130) is made of any one of glycerol, chicory fiber, resistant dextrin, oat fiber, oligosaccharide, microcrystalline cellulose, corn starch, lecithin and trehalose.

4. The Lactobacillus oral granules for improving exercise ability according to claim 1, characterized in that: The odor barrier layer (140) is made of any one of diglyceride, monoglyceride, green tea powder, monk fruit extract, yeast extract, tea extract, orange peel extract and lemon peel extract.

5. The Lactobacillus oral granules for improving exercise ability according to claim 1, characterized in that: The flow-aiding layer (150) is made of any one of magnesium stearate, silicon dioxide, calcium hydrogen phosphate, microcrystalline cellulose, micropowder silica gel, lactose, fatty acid glycosides and fatty acid sucrose glycosides.

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