Circulating DNA synthesis device

Abstract

The utility model discloses a circulating DNA (deoxyribonucleic acid) synthesis device which comprises a synthesis column, a reaction cavity is formed in the synthesis column, and the bottom of the reaction cavity is higher than that of the synthesis column; the reagent buffer chamber is provided with a switching valve, a balance electromagnetic valve, a vacuum outlet, a circulating electromagnetic valve, a circulating outlet, a liquid inlet and an air inlet, wherein the switching valve is communicated with the bottom of the synthetic column through a pipeline; the balance electromagnetic valve is respectively connected with the synthetic column and the switching valve; the interior of the vacuum outlet is connected with the balance electromagnetic valve and the switching valve; according to the utility model, the circulating liquid is arranged, so that the synthetic liquid passes through the synthetic column for multiple times, the synthetic efficiency of equipment is greatly improved, the consumption of reagents is greatly reduced on the premise of not changing a synthetic carrier plate and a process, the equivalent weight of the reagents in normal single flowing is more than 15 times, the consumption of the reagents can be reduced to 5-10 times, and the effect is obvious.

Description

Technical Field

[0001] The utility model relates to biosynthesis equipment, in particular to a cyclic DNA synthesis device. Background Art

[0002] Gene synthesis is a frontier field and an important part of biosynthesis in recent years. With the further development of synthetic biology, gene synthesis, as its underlying technology, has had a significant impact on disease treatment, environmental governance, new drugs, new materials, new energy and other fields. Gene synthesis is a method of synthesizing double-stranded DNA in vitro and is one of the means to obtain target genes. Compared with traditional cloning technology, gene synthesis is fast and efficient, and can achieve on-demand synthesis and directional modification of biological macromolecules, so it has been widely used.

[0003] At present, the more mature and widely used DNA synthesis process in the market is realized by using a column DNA synthesizer. Specifically, the DNA synthesis process occurs on the synthesis column of the DNA synthesizer. The basic principle of DNA synthesis is: DNA is a chemical molecule composed of four bases (A / T / G / C) arranged and combined. The synthesis of DNA refers to connecting specific bases (such as AATGAC) in sequence on the initial DNA (a chemical molecule formed by connecting a small amount of bases). Specifically, the DNA synthesis process is composed of multiple cycles, and each cycle only achieves the purpose of connecting one base on the initial DNA. Each cycle is as follows: first, the initial DNA (a small amount of oligonucleotides) is formed inside the synthesis column, and then a plurality of DNA synthesis reagents are controlled to pass through the synthesis column in sequence. Some of the synthesis reagents react chemically with the initial DNA (i.e., connect one base), thereby achieving the purpose of DNA synthesis, and after the reagents pass through the synthesis column, they all flow into the waste liquid tank and are treated as waste materials. The reagent consumption is large and the cost is high. Utility Model Content

[0004] In view of the problem of large reagent consumption during DNA synthesis, the utility model provides a cyclic DNA synthesis device, which uses a circulating liquid setting to allow the synthesis liquid to pass through a synthesis carrier column multiple times, thereby reducing the actual consumption of reagents and lowering costs.

[0005] In order to achieve the above object, the utility model provides a cyclic DNA synthesis device, which comprises:

[0006] A synthesis column, wherein a reaction cavity is formed inside, and the bottom of the reaction cavity is higher than the bottom of the synthesis column;

[0007] Reagent cache room, which is equipped with:

[0008] A switching valve is connected to the bottom of the synthesis column through a pipeline,

[0009] A balancing solenoid valve is connected to the synthesis column and the switching valve respectively.

[0010] The vacuum outlet is internally connected with the balancing solenoid valve and the switching valve.

[0011] A circulation solenoid valve is connected to the switching valve.

[0012] The circulation outlet is internally connected to the circulation solenoid valve and externally connected to the liquid inlet.

[0013] Air intake.

[0014] Preferably, a reagent feed piece is provided above the synthesis column, and a first inlet, a second inlet and an outlet which are interconnected are provided on the reagent feed piece, and the second inlet is connected to the circulation outlet.

[0015] Further preferably, the outlet is connected to a liquid outlet pipe, and the liquid outlet end of the liquid outlet pipe is located above the synthesis column.

[0016] Preferably, there is one vacuum outlet, the balancing solenoid valve and the switching valve are connected by a pipeline, and the vacuum outlet is communicated with the pipeline.

[0017] Preferably, there are two vacuum outlets, and the two vacuum outlets are respectively connected to the balancing solenoid valve and the switching valve.

[0018] Preferably, the synthesis column has one or more reaction chambers.

[0019] Specifically, the outside of the vacuum outlet is connected to a negative pressure pump.

[0020] Preferably, a check valve is provided on the pipeline.

[0021] Through the above technical solution, the utility model achieves the following beneficial effects:

[0022] The utility model adopts a circulating liquid setting to make the synthetic liquid pass through the synthesis column multiple times, which greatly improves the synthesis efficiency of the equipment and greatly reduces the consumption of reagents without changing the synthesis carrier and the process. The normal single flow equivalent is more than 15 times the reagent consumption, which can be reduced to 5-10 times in this way, with a significant effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 This is a structural schematic diagram of a preferred embodiment of the cyclic DNA synthesis device of the utility model;

[0024] Figure 2 yes Figure 1A top view of

[0025] Figure 3 yes Figure 1 Side view of

[0026] Figure 4 yes Figure 1 AA section view;

[0027] Figure 5 yes Figure 1 Stereoscopic image of

[0028] Figure 6 It is a structural schematic diagram of a preferred embodiment of the reagent feeding member of the utility model;

[0029] Figure 7 yes Figure 6 Schematic diagram from another direction;

[0030] Figure 8 A schematic structural diagram of another preferred embodiment of the cyclic DNA synthesis device of the present invention.

[0031] Description of Reference Numerals

[0032] 1 synthesis column, 11 reaction chamber, 2 reagent buffer chamber, 3 switching valve, 31 pipeline, 32 check valve, 4 balance solenoid valve, 5 vacuum outlet, 6 circulation solenoid valve, 7 circulation outlet, 8 air inlet, 9 reagent feed piece, 91 first inlet, 92 second inlet, 93 liquid outlet pipe. DETAILED DESCRIPTION

[0033] The specific implementation of the present invention is described in detail below in conjunction with the accompanying drawings. It should be understood that the specific implementation described here is only used to illustrate and explain the present invention, and is not used to limit the present invention.

[0034] First of all, it should be noted that in the following description, some directional words involved in order to clearly illustrate the technical solution of the utility model, such as "external", "internal", etc., all have the meaning by analogy with the normal directions of the components in the cyclic DNA synthesis device. For example, the part through which the liquid passes is the internal part, and the part opposite to it is the external part. It is only for the convenience of describing the utility model and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore cannot be understood as a limitation on the utility model.

[0035] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the said features.

[0036] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installation" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a direct connection, or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0037] Example 1

[0038] like Figure 1-Figure 7 As shown, the cyclic DNA synthesis device described in the utility model comprises:

[0039] The synthesis column 1 has a reaction chamber 11 formed inside for accommodating the synthesis carrier, and the bottom of the reaction chamber 11 is higher than the bottom of the synthesis column 1;

[0040] Reagent cache chamber 2, which is provided with:

[0041] The switching valve 3 is connected to the bottom of the synthesis column 1 through a pipeline 31.

[0042] The balance solenoid valve 4 is connected to the synthesis column 1 and the switching valve 3 respectively.

[0043] The vacuum outlet 5 is internally connected to the balance solenoid valve 4 and the switching valve 3.

[0044] The circulation solenoid valve 6 is connected to the switching valve 3.

[0045] The circulation outlet 7 is internally connected to the circulation solenoid valve 6 and externally connected to the liquid inlet.

[0046] Air Inlet 8.

[0047] The DNA synthesis process is divided into liquid injection, reaction, circulation, and rapid vacuuming. The traditional synthesis method is to directly discharge it through the reaction carrier once, while the utility model allows it to circulate through the carrier multiple times. The working process of the circular DNA synthesis device of the utility model is as follows:

[0048] Liquid injection: inject reagents through the liquid injection outlet assembly (existing assembly can be used);

[0049] Reaction: the air inlet 8 is closed, the balance solenoid valve 4 is opened, the switching valve 3 switches the circulation solenoid valve 6 to pass, the circulation solenoid valve 6 is closed, and the air is slowly pumped out through the vacuum outlet 5 to control the column to slowly descend;

[0050] Circulation: Keep the circulation solenoid valve 6 closed, close the balance solenoid valve 4 and the circulation outlet 7, and slowly inject gas through the air inlet 8 to push the liquid buffered in the reagent buffer chamber 2 to rise from the pipeline 31 to the synthesis column 1;

[0051] If you need to recycle, repeat the reaction-cycle two steps;

[0052] Rapid liquid extraction: Keep the valve state of the above cycle, close the air inlet 8, switch the switching valve 3 to connect the balance solenoid valve 4, and quickly drain the liquid in the column from the vacuum outlet 5. Specifically, the outside of the vacuum outlet 5 is connected to a negative pressure pump.

[0053] The utility model adopts a circulating liquid setting to make the synthetic liquid pass through the synthesis column 1 multiple times, which greatly improves the synthesis efficiency of the equipment and greatly reduces the consumption of reagents without changing the synthesis carrier and process. The normal single flow equivalent is more than 15 times the reagent consumption, which can be reduced to 5-10 times in this way, with significant effect.

[0054] Specifically, a reagent feeder 9 is provided above the synthesis column 1, and a first inlet 91, a second inlet 92 and an outlet are provided on the reagent feeder 9, and the second inlet 92 is connected to the circulation outlet 7. The liquid injection outlet assembly is connected to the first inlet 91, so that when liquid injection is required, normal liquid injection is achieved through the first inlet 91, and when circulation is required, the circulating liquid is achieved through the second inlet 92.

[0055] In order to facilitate liquid inlet, the outlet is connected to a liquid outlet pipe 93 , and the liquid outlet end of the liquid outlet pipe 93 is located above the synthesis column 1 .

[0056] As a preferred embodiment of the present utility model, there is one vacuum outlet 5, the balancing solenoid valve 4 and the switching valve 3 are connected by a pipeline, and the vacuum outlet 5 is communicated with the pipeline.

[0057] Of course, there may be two vacuum outlets 5 , which are respectively connected to the balancing solenoid valve 4 and the switching valve 3 . The exhaust of the balancing solenoid valve 4 and the draining of the switching valve 3 are respectively achieved through different vacuum outlets 5 .

[0058] On the basis of the above technical solution, the reaction chamber 11 in the synthesis column 1 has one or more, and the liquid outlet end of the liquid inlet is directly aligned with the mouth of the reaction chamber 11 for dripping. In a preferred embodiment, the circulation outlet 7 is connected to the second inlet 92 through a pipeline, and the reagent feeding member 9 is configured to be slidable, so that the liquid outlet pipe 93 can add liquid to the reaction chamber 11 one by one. Figure 1 This is just an example configuration.

[0059] Example 2

[0060] like Figure 8 As shown, on the basis of Example 1, a check valve 32 is provided on the pipeline 31, and the liquid does not circulate through the pipeline 31, but circulates through the circulation outlet 7 connected to the upper circulation liquid inlet. This embodiment adopts the following method to circulate the reagent: keep the circulation solenoid valve 6 closed, close the balance solenoid valve 4, open the circulation outlet 7, slowly inject gas through the air inlet 8 to push the liquid buffered in the reagent buffer chamber 2 to rise from the circulation outlet 7 to the liquid inlet again, and enter the synthesis column 1.

[0061] The preferred embodiments of the present invention are described in detail above in conjunction with the accompanying drawings. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, the technical solution of the present invention can be subjected to a variety of simple modifications, and these simple modifications all belong to the protection scope of the present invention.

[0062] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present utility model will not further describe various possible combinations.

[0063] In addition, various embodiments of the present invention may be arbitrarily combined, and as long as they do not violate the concept of the present invention, they should also be regarded as the contents disclosed by the present invention.

Claims

1. A cyclic DNA synthesis device, characterized in that: include: A synthesis column (1) having a reaction chamber (11) formed therein, wherein the bottom of the reaction chamber (11) is higher than the bottom of the synthesis column (1); The reagent buffer chamber (2) is provided with: The switching valve (3) is connected to the bottom of the synthesis column (1) through a pipeline (31). A balancing solenoid valve (4) is connected to the synthesis column (1) and the switching valve (3) respectively. The vacuum outlet (5) is internally connected to the balancing solenoid valve (4) and the switching valve (3). A circulation solenoid valve (6) is connected to the switching valve (3). The circulation outlet (7) is internally connected to the circulation solenoid valve (6) and externally connected to the liquid inlet. Air inlet (8).

2. The cyclic DNA synthesis device according to claim 1, characterized in that: A reagent feed piece (9) is provided above the synthesis column (1), and the reagent feed piece (9) is provided with a first inlet (91), a second inlet (92) and an outlet which are interconnected, and the second inlet (92) is connected to the circulation outlet (7).

3. The cyclic DNA synthesis device according to claim 2, characterized in that: The outlet is connected to a liquid outlet pipe (93), and the liquid outlet end of the liquid outlet pipe (93) is located above the synthesis column (1).

4. The cyclic DNA synthesis device according to claim 1, characterized in that: The vacuum outlet (5) has one, the balancing solenoid valve (4) and the switching valve (3) are connected via a pipeline, and the vacuum outlet (5) is in communication with the pipeline.

5. The cyclic DNA synthesis device according to claim 1, characterized in that: There are two vacuum outlets (5), and the two vacuum outlets (5) are respectively connected to the balancing solenoid valve (4) and the switching valve (3).

6. The cyclic DNA synthesis device according to claim 1, characterized in that: The synthesis column (1) has one or more reaction chambers (11).

7. The cyclic DNA synthesis device according to claim 1, characterized in that: The outside of the vacuum outlet (5) is connected to a negative pressure pump.

8. The cyclic DNA synthesis device according to any one of claims 1 to 7, characterized in that: The pipeline (31) is provided with a check valve (32).

Images