Portable nucleic acid detection device

Abstract

The utility model discloses a portable nucleic acid detection device which comprises an outer sleeve shell, a sample adding opening is formed in the upper end of the outer sleeve shell, and a sample adding cover capable of being opened and closed is arranged on the sample adding opening; the functional part is movably arranged in the outer sleeve shell between an upper position and a lower position, the outer sleeve shell is internally divided into an upper chamber and a lower chamber, the upper chamber is internally provided with a cracking buffer solution, the functional part is internally provided with a microfluidic flow channel, a reaction cavity and an exhaust channel, the microfluidic flow channel is communicated with the upper chamber and the reaction cavity, and the exhaust channel is communicated with the reaction cavity. The exhaust channel is respectively communicated with the lower chamber and the reaction cavity; the liquid adding sealing film is arranged between the microfluidic flow channel and the upper chamber in a sealing manner; the puncture part is arranged in the upper cavity, when the functional part is located at the upper position, the puncture part punctures the liquid adding sealing film, and when the functional part is located at the lower position, the puncture part avoids the liquid adding sealing film. The portable nucleic acid detection device disclosed by the embodiment of the utility model has the advantages of high portability, convenience and rapidness in operation, strong applicability, difficulty in secondary pollution and the like.

Description

Technical Field

[0001] The utility model relates to the technical field of nucleic acid detection, and in particular to a portable nucleic acid detection device. Background Art

[0002] The molecular point-of-care (POCT) system is a fully automatic, integrated, closed molecular biology detection system. Its nucleic acid extraction and amplification detection are all completed on the same closed, automated instrument. There is no need for manual preparation of reagents or manual processing of samples. No other manual operations are required from the time the sample is loaded onto the machine to the time the result is reported. This can effectively prevent secondary contamination of biological samples and their genetic material and the environmental pollution caused by them.

[0003] The molecular instant diagnostic equipment in related technologies is large in size, difficult to be portable, and cannot meet the needs of self-testing diseases at home. Utility Model Content

[0004] The utility model aims to solve at least one of the technical problems existing in the prior art. To this end, the utility model proposes a portable nucleic acid detection device, which has the advantages of high portability, convenient and fast operation, strong applicability, and low secondary contamination.

[0005] To achieve the above-mentioned purpose, according to an embodiment of the utility model, a portable nucleic acid detection device is proposed, and the portable nucleic acid detection device includes: an outer shell, a sample loading port is provided at the upper end of the outer shell, and an openable and closable sample loading cover is provided on the sample loading port; a functional part, the functional part is movably arranged in the outer shell between an upper position and a lower position and separates an upper chamber and a lower chamber in the outer shell, a lysis buffer is provided in the upper chamber, a microfluidic flow channel, a reaction chamber and an exhaust channel are provided in the functional part, the microfluidic flow channel is communicated with the upper chamber and the reaction chamber respectively, and the exhaust channel is communicated with the lower chamber and the reaction chamber respectively; a liquid-adding sealing film, the liquid-adding sealing film is sealed between the microfluidic flow channel and the upper chamber; a puncture part, the puncture part is arranged in the upper chamber, the puncture part punctures the liquid-adding sealing film when the functional part is in the upper position, and the puncture part avoids the liquid-adding sealing film when the functional part is in the lower position.

[0006] The portable nucleic acid detection device according to the embodiment of the utility model has the advantages of high portability, convenient and fast operation, strong applicability, and not easy to be contaminated by secondary pollution.

[0007] In addition, the portable nucleic acid detection device according to the above embodiment of the utility model may also have the following additional technical features:

[0008] According to one embodiment of the utility model, a guide edge is provided in the outer shell around the sample loading port, the diameter of the guide edge gradually decreases from top to bottom, a puncturable sample loading sealing film is provided at the lower end of the guide edge, the guide edge and the sample loading sealing film together define a sample loading groove, and a freeze-dried lysis reagent is provided in the sample loading groove.

[0009] According to an embodiment of the present invention, a reagent chamber connecting the reaction chamber and the microfluidic channel is provided between the reaction chamber and the microfluidic channel, and a freeze-dried reaction reagent is provided in the reagent chamber.

[0010] According to an embodiment of the present invention, a heating element for heating the reaction chamber is provided outside the reaction chamber.

[0011] According to an embodiment of the utility model, a waste liquid chamber is further provided in the functional part, the waste liquid chamber is communicated with the microfluidic channel, and the waste liquid chamber is communicated with the lower chamber through an exhaust channel.

[0012] According to one embodiment of the utility model, a sealing member is provided on the puncture member, and when the functional member is in the lower position, the sealing member is separated from the functional member, and when the functional member is in the upper position, the sealing member abuts against the functional member to seal the upper chamber and the microfluidic channel.

[0013] According to an embodiment of the utility model, a push block is provided on the functional part, and a avoidance opening is provided on the outer shell, and the push block is exposed from the avoidance opening.

[0014] According to an embodiment of the utility model, a stopper is detachably engaged in the avoidance opening, and when the stopper is engaged in the avoidance opening, the push block is stopped to limit the upward movement of the functional part, and a pinching structure is provided on the stopper.

[0015] According to an embodiment of the present invention, the microfluidic channel has an S-shaped anti-backflow section.

[0016] According to an embodiment of the present invention, a breathable and water-proof member is provided between the exhaust passage and the reaction chamber.

[0017] Additional aspects and advantages of the present invention will be given in part in the following description, and in part will become apparent from the following description, or will be learned through the practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] The above and / or additional aspects and advantages of the present invention will become apparent and easily understood from the description of the embodiments in conjunction with the following drawings, in which:

[0019] Figure 1It is a schematic diagram of the structure of a portable nucleic acid detection device according to an embodiment of the utility model, wherein the functional parts are located in the lower position.

[0020] Figure 2 It is a schematic diagram of the structure of a portable nucleic acid detection device according to an embodiment of the utility model, wherein the functional parts are located in the lower position.

[0021] Figure 3 It is a schematic diagram of the structure of a portable nucleic acid detection device according to an embodiment of the utility model, wherein the functional parts are located between the upper position and the lower position.

[0022] Figure 4 It is a schematic diagram of the structure of a portable nucleic acid detection device according to an embodiment of the utility model, in which the functional parts are located in the upper position.

[0023] Figure numerals: portable nucleic acid detection device 1, outer shell 10, sample loading port 11, upper chamber 12, lower chamber 13, lysis buffer 14, sample loading cover 15, avoidance port 16, functional part 20, microfluidic channel 21, reaction chamber 22, exhaust channel 23, reagent chamber 24, waste liquid chamber 25, anti-backflow section 26, breathable water-proof part 27, liquid loading sealing film 30, puncture part 40, sealing part 41, guide edge 50, sample loading sealing film 51, freeze-dried lysis reagent 52, push block 60, stop block 70, pinching structure 71. DETAILED DESCRIPTION

[0024] This application is based on the inventor's discovery and understanding of the following facts and problems:

[0025] The molecular instant diagnostic equipment in related technologies is large in size, difficult to be portable, and cannot meet the needs of self-testing diseases at home.

[0026] The more classic nucleic acid amplification method in the molecular POCT system is the polymerase chain reaction (PCR), whose exponential nucleic acid amplification can amplify the target nucleic acid sequence tens of millions of times in a short period of time. Fluorescence quantitative polymerase chain reaction technology (qPCR) combines spectral technology to achieve high sensitivity, specificity and accuracy of nucleic acid amplification and detection. However, the qPCR reaction process requires multiple cycles of heating and cooling, and the detection process requires fluorescence detection equipment. The overall equipment requirements are large, the operation is complicated, and professional PCR laboratories and operators are required. It has scene restrictions and cannot meet the growing immediacy needs of molecular POCT.

[0027] Isothermal amplification technology has a constant reaction temperature and visualized amplification results. It has low requirements for equipment and environment and is more suitable for application scenarios with relatively poor facilities, environment and professionalism.

[0028] The embodiments of the present invention are described in detail below, and examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and cannot be understood as limiting the present invention.

[0029] In the description of the present utility model, it is necessary to understand that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present utility model. In addition, features defined as "first" and "second" may explicitly or implicitly include one or more of such features. In the description of the present utility model, unless otherwise specified, "multiple" means two or more.

[0030] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installation", "connection" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0031] The portable nucleic acid detection device 1 according to an embodiment of the present utility model is described below with reference to the accompanying drawings.

[0032] like Figure 1-Figure 4 As shown, the portable nucleic acid detection device 1 according to an embodiment of the utility model includes an outer shell 10, a functional part 20, a liquid-filling sealing film 30 and a puncture part 40.

[0033] The upper end of the outer shell 10 is provided with a sample loading port 11 (the up and down directions are shown by the arrows in the figure), and the sample loading port 11 is provided with an openable and closable sample loading cover 15. The functional part 20 is movably arranged in the outer shell 10 between the upper position and the lower position and separates the upper chamber 12 and the lower chamber 13 in the outer shell 10. The upper chamber 12 is provided with a lysis buffer 14. The functional part 20 is provided with a microfluidic flow channel 21, a reaction chamber 22 and an exhaust channel 23. The microfluidic flow channel 21 is connected to the upper chamber 12 and the reaction chamber 22 respectively, and the exhaust channel 23 is connected to the lower chamber 13 and the reaction chamber 22 respectively. The liquid-adding sealing film 30 is sealed between the microfluidic flow channel 21 and the upper chamber 12. The puncture member 40 is arranged in the upper chamber 12. When the functional part 20 is in the upper position, the puncture member 40 punctures the liquid-adding sealing film 30, and when the functional part 20 is in the lower position, the puncture member 40 avoids the liquid-adding sealing film 30.

[0034] Specifically, if Figure 1-Figure 4 As shown, the sample addition port 11 is connected to the upper chamber 12, and the functional component 20 is arranged in the outer shell 10 so as to be movable up and down between the upper position and the lower position. The upper chamber 12 is located above the functional component 20, and the lower chamber 13 is located below the functional component 20.

[0035] In the initial state, the functional part 20 is located in the lower position, such as Figure 1 and Figure 2 As shown, at this time, the puncture member 40 avoids the liquid-adding sealing film 30 and does not contact the liquid-adding sealing film 30 . The lysis buffer 14 in the upper chamber 12 and the microfluidic channel 21 in the functional part 20 are separated from each other by the liquid-adding sealing film 30 .

[0036] When in use, first use a sampling swab to collect a sample, which can be saliva, urine, tissue fluid, etc.

[0037] Open the sample loading cover 15 and insert the sampling swab into the upper chamber 12 through the sample loading port 11 , immerse the lower end of the sampling swab in the lysis buffer 14 of the upper chamber 12 , stir or break the lower end of the sampling swab in the lysis buffer 14 .

[0038] Close the sample adding cover 15, move the functional part 20 upward, such as Figure 3 As shown, during the movement, the liquid-adding sealing film 30 is punctured by the puncture member 40, connecting the upper chamber 12 and the microfluidic channel 21. During the upward movement of the functional part 20, the volume of the upper chamber 12 decreases and the pressure increases, while the volume of the lower chamber 13 increases and the pressure decreases. The lysis buffer 14 in the upper chamber 12 flows into the microfluidic channel 21 and further flows into the reaction chamber 22 under pressure. During the process, the gas will be discharged to the lower chamber 13 through the exhaust channel 23 to balance the air pressure. Until the functional part 20 reaches the upper position, as shown in FIG. Figure 4 As shown, the lysis buffer 14 in the upper chamber 12 completely flows into the functional part 20 .

[0039] For example, a freeze-dried lysis reagent 52 can be set at the sample loading port 11, and a freeze-dried reaction reagent can be set at the entrance of the reaction chamber 22. During the sample loading process, the sampling swab carries the freeze-dried lysis reagent 52 and mixes with the lysis buffer 14. After flowing into the microfluidic channel 21, it is further mixed with the freeze-dried reaction reagent and then enters the reaction chamber 22. The reaction chamber 22 is heated at a constant temperature to start the constant temperature amplification reaction.

[0040] Finally, the result is judged by detecting the signal of the reaction product or visually observing the state of the reaction product.

[0041] This allows constant temperature nucleic acid amplification, active microfluidics technology and visualization detection / fluorescence detection technology of amplification products to be combined to realize the process of sample lysis, nucleic acid amplification, nucleic acid detection, and result judgment, thereby achieving nucleic acid detection of related diseases.

[0042] According to the portable nucleic acid detection device 1 of the embodiment of the utility model, only manual sampling and adding of samples and moving the functional part 20 are required, and the molecular instant detection of the sample can be completed without the auxiliary operation of other external instruments and equipment. Compared with the molecular instant diagnosis equipment in the related art, it can complete the molecular instant detection by itself, is portable, and can be used in occasions such as home disease self-testing or on-site testing, and has strong applicability.

[0043] Furthermore, by movably arranging the functional part 20 between an upper position and a lower position in the outer shell 10 and separating the upper chamber 12 and the lower chamber 13 in the outer shell 10, the liquid-filled sealing film 30 can be punctured during the upward movement of the functional part 20, and the pressures of the upper chamber 12 and the lower chamber 13 can be changed, thereby prompting the solution in the upper chamber 12 to be quickly transferred to the reaction chamber 22 of the functional part 20 for reaction. The entire process is simple and fast to operate, and only the functional part 20 needs to be moved without an additional power system. The liquid flow transfer can be completed manually without being restricted by other external instruments and equipment. The process has strong applicability and is convenient for portable on-site testing or home self-testing.

[0044] In addition, by providing a sample loading cover 15 and closing the sample loading cover 15 after sample loading, on the one hand, the upper chamber 12 can be closed so that the air pressure in the upper chamber 12 can drive the lysis buffer 14 to enter the functional parts, and on the other hand, the sample loading cover 15 is closed after sample loading to seal the outer shell 10. Since the sample loading and post-sample loading processes are completed in the outer shell 10, the outer shell 10 is isolated from the external environment after sample loading, which can effectively prevent aerosol contamination generated by the reaction, and there is no secondary contamination of the solution and pollution of the environment by the amplification product, which further facilitates the realization of portable detection.

[0045] Therefore, the portable nucleic acid detection device 1 according to the embodiment of the utility model has the advantages of high portability, convenient and fast operation, strong applicability, and not easy to be contaminated by secondary pollution.

[0046] The portable nucleic acid detection device 1 according to a specific embodiment of the present utility model is described below with reference to the accompanying drawings.

[0047] In some specific embodiments of the present invention, Figure 1-Figure 4 As shown, the portable nucleic acid detection device 1 according to an embodiment of the utility model includes an outer shell 10, a functional part 20, a liquid-filling sealing film 30 and a puncture part 40.

[0048] Specifically, if Figure 1-Figure 4 As shown, a guide edge 50 is provided around the sample loading port 11 in the outer shell 10, and the guide edge 50 gradually decreases in diameter from top to bottom. The lower end of the guide edge 50 is provided with a sample loading sealing film 51 that can be pierced. The guide edge 50 and the sample loading sealing film 51 jointly define a sample loading groove, and a freeze-dried lysis reagent 52 is provided in the sample loading groove. Specifically, the sample loading sealing film 51 can be tin foil. In this way, the sample loading sealing film 51 can be used to seal the freeze-dried lysis reagent 52 and the lysis buffer 14. When loading, the sample loading sealing film 51 is pierced by a sampling swab, so that the freeze-dried lysis reagent 52 falls into the lysis buffer 14 to form a lysate. Thus, not only can the freeze-dried lysis reagent 52 be separated from the lysis buffer 14 before loading, but also the lysis buffer 14 and the freeze-dried lysis reagent 52 can be mixed during the sample loading process, making the operation process simpler and faster.

[0049] More specifically, Figure 2-Figure 4 As shown, a reagent chamber 24 is provided between the reaction chamber 22 and the microfluidic channel 21, and the reagent chamber 24 is provided with a freeze-dried reaction reagent. In this way, the lysate formed by mixing the lysing buffer 14 and the freeze-dried lysing reagent 52 after the addition of the sample can be mixed with the freeze-dried reaction reagent in the process of entering the microfluidic channel 21, so that all reactants can react in the reaction chamber 22.

[0050] Furthermore, a heating element for heating the reaction chamber 22 is provided outside the reaction chamber 22. Specifically, the heating element may be an electric heating element, which may be powered by a built-in battery or an external power supply, and a switch for controlling the heating element may also be provided on the outer shell 10. This facilitates the conduction of the isothermal amplification reaction.

[0051] Specifically, the freeze-dried reaction reagents are enzymes, primers, probes, deoxyribonucleotide triphosphates (dNTPs), etc. required for one-step isothermal amplification of nucleic acids. The heating element can be set according to the required temperature of the reagent. Here, preferably, isothermal amplification can be selected from recombinase polymerase amplification reaction (RPA), the reaction temperature is 37-42 degrees Celsius, the heating element can be selected from 40 degrees Celsius, or loop-mediated isothermal amplification (LAMP), the reaction temperature is 62-67 degrees Celsius, and the heating element can be selected from 65 degrees Celsius.

[0052] In some embodiments, a signal detection element is further provided outside the reaction chamber 22 for detecting signals of the solution after amplification in the reaction chamber 22. The signal detection element is adapted to the reagent system and can be selected to detect fluorescence signals, turbidity signals or color change signals.

[0053] In other embodiments, the nucleic acid amplification results of the reaction chamber 22 can also be detected by visual methods, by observing the color change of the solution before and after nucleic acid amplification or by placing a nucleic acid detection test strip in the reaction chamber 22, the detection results can be judged by naked eyes and the presence of a certain disease virus can be evaluated.

[0054] Figure 1-Figure 4 The portable nucleic acid detection device 1 according to some examples of the utility model is shown. Figure 2-Figure 4 As shown, a waste liquid chamber 25 is further provided in the functional part 20, the waste liquid chamber 25 is communicated with the microfluidic flow channel 21, and the waste liquid chamber 25 is communicated with the lower chamber 13 through the exhaust channel 23. In this way, the excess lysate can enter the waste liquid chamber 25, so that the lysate buffer 14 pre-set in the upper chamber 12 can leave a margin for filling the reaction chamber 22, so as to ensure that the reaction chamber 22 is filled with the lysate and ensure the reliable conduct of the reaction.

[0055] Specifically, there can be multiple reaction chambers 22, the microfluidic flow channel 21 can include a main channel, a waste liquid branch and multiple reaction chamber branches, the liquid adding sealing film 30 is sealed at the upper end of the main channel, the waste liquid branch is respectively connected to the main channel and the waste liquid chamber 25, and the reaction chamber branch is respectively connected to the reaction chamber 22 and the main channel. There can be multiple exhaust channels 23 and they are respectively connected to the waste liquid chamber 25 and multiple reaction chambers 22.

[0056] Advantageously, if Figure 2-Figure 4 As shown, the microfluidic channel 21 has an S-shaped anti-backflow section 26. Specifically, each reaction chamber branch has an anti-backflow section 26. This can prevent the solution from flowing back.

[0057] More advantageously, if Figure 2-Figure 4As shown, a breathable water-blocking member 27 is provided between the exhaust channel 23 and the reaction chamber. Specifically, the breathable water-blocking member 27 can be a polyethylene sintered filter element. A breathable water-blocking member 27 is provided at the inlet end of each exhaust channel 23. In this way, the breathable water-blocking member 27 can be used to block the outflow of the solution, and the gas can be discharged to balance the air pressure, so that the solution can flow under the drive of the movement of the functional part 20 and prevent the solution from flowing out of the reaction chamber 22.

[0058] More advantageously, if Figure 2-Figure 4 As shown, a sealing member 41 is provided on the puncture member 40. When the functional member 20 is in the lower position, the sealing member 41 is separated from the functional member 20. When the functional member 20 is in the upper position, the sealing member 41 abuts against the functional member 20 to seal the upper chamber 12 and the microfluidic channel 21. Specifically, the sealing member 41 is provided in the middle of the puncture member 40. In this way, the sealing member 41 can be used to seal the microfluidic channel 21 after the lysis buffer 14 flows into the functional member 20, thereby further preventing aerosol pollution generated by the reaction, and further preventing secondary pollution and pollution to the environment.

[0059] Specifically, Figure 1 , Figure 3 and Figure 4 As shown, a push block 60 is provided on the functional part 20, and a clearance opening 16 is provided on the outer shell 10, and the push block 60 is exposed from the clearance opening 16. In this way, the functional part 20 can be moved by pushing the push block 60, which is convenient for pushing the functional part 20 and is convenient for manual operation.

[0060] More specifically, Figure 1 As shown, a stopper 70 is detachably fitted in the avoidance opening 16. When the stopper 70 is fitted in the avoidance opening 16, the pusher 60 is stopped to limit the upward movement of the functional part 20. A pinching structure 71 is provided on the stopper 70. In this way, the stopper 70 can be used to prevent the functional part 20 from moving at will and puncturing the liquid-adding sealing film 30. The pinching structure 71 can facilitate the removal of the stopper 70 from the avoidance opening 16, further facilitating manual operation.

[0061] Reference below Figure 1-Figure 4 The operation process of the portable nucleic acid detection device 1 according to some examples of the present utility model is described.

[0062] In the initial state, Figure 1 and Figure 2 As shown, the functional part 20 is located at the lower position, and the push block 60 is located at the bottom of the avoidance port 16. At this time, the puncture member 40 is not in contact with the liquid sealing film 30 on the top of the functional part 20, and the lysis buffer 14 in the upper chamber 12 and the microfluidic channel 21 in the functional part 20 are separated from each other.

[0063] Use a sampling swab to collect samples, which can be saliva, urine, tissue fluid, etc.

[0064] Open the sample loading cover 15, and insert the sampling swab into the upper chamber 12 through the sample loading port 11 to pierce the sample loading sealing film 51. At this time, the freeze-dried lysis reagent 52 set above the sample loading sealing film 51 can fall into the lysis buffer 14 to dissolve, and immerse the front end of the sampling swab in the lysis solution. Repeatedly stir to elute the sample, or directly break off the front end of the swab and leave the sampling head in the lysis solution.

[0065] After the sample addition is completed, the sample addition cover 15 is closed.

[0066] The stopper 70 is removed from the escape opening 16 .

[0067] Push the push block 60 upward, and the functional part 20 moves upward. During the movement, the liquid-adding sealing film 30 is punctured by the puncture piece 40, connecting the upper chamber 12 and the microfluidic channel 21. During the upward movement of the functional part 20, the volume of the upper chamber 12 decreases and the pressure increases, while the volume of the lower chamber 13 increases and the pressure decreases. The lysate in the upper chamber 12 flows into the microfluidic channel 21 of the functional part 20 and is further diverted to the reaction chamber 22 and the waste liquid chamber 25, and is finally blocked in the reaction chamber 22 by the breathable water-proof part 27. During the process, the gas will be discharged to the lower chamber 13 through the exhaust channel 23 to balance the air pressure. Until the functional part 20 reaches the upper position, the sealing part 41 on the puncture piece 40 fits with the upper end of the functional part 20 to seal the microfluidic channel 21 and the upper chamber 12, playing the role of secondary sealing, which can effectively prevent aerosol contamination generated by the nucleic acid amplification reaction.

[0068] The heating element is activated by turning on the power supply and / or opening the switch to heat the reaction chamber 22 and perform constant temperature amplification of nucleic acid.

[0069] After a certain period of heating, the detection element is activated to detect the signal of the reaction product or the state of the reaction product is visually observed to determine the result.

[0070] Other structures and operations of the portable nucleic acid detection device 1 according to the embodiment of the utility model are known to ordinary technicians in the field and will not be described in detail here.

[0071] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "illustrative embodiments", "examples", "specific examples", or "some examples" means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the utility model. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0072] Although the embodiments of the present invention have been shown and described, those skilled in the art will appreciate that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the claims and their equivalents.

Claims

1. A portable nucleic acid detection device, characterized in that: include: An outer shell, wherein the upper end of the outer shell is provided with a sample loading port, and the sample loading port is provided with an openable and closable sample loading cover; A functional part, wherein the functional part is movably arranged in the outer shell between an upper position and a lower position and an upper chamber and a lower chamber are separated in the outer shell, a lysis buffer is arranged in the upper chamber, a microfluidic flow channel, a reaction chamber and an exhaust channel are arranged in the functional part, the microfluidic flow channel is communicated with the upper chamber and the reaction chamber respectively, and the exhaust channel is communicated with the lower chamber and the reaction chamber respectively; A liquid-adding sealing film, wherein the liquid-adding sealing film is sealed between the microfluidic channel and the upper chamber; The puncture member is arranged in the upper chamber. When the functional member is in the upper position, the puncture member punctures the liquid-adding sealing film. When the functional member is in the lower position, the puncture member avoids the liquid-adding sealing film.

2. The portable nucleic acid detection device according to claim 1, characterized in that: A guide edge is arranged around the sample loading port in the outer shell, and the diameter of the guide edge gradually decreases from top to bottom. A puncturable sample loading sealing film is arranged at the lower end of the guide edge. The guide edge and the sample loading sealing film jointly define a sample loading groove, and a freeze-dried lysis reagent is arranged in the sample loading groove.

3. The portable nucleic acid detection device according to claim 1, characterized in that: A reagent chamber connecting the reaction chamber and the microfluidic channel is provided between the reaction chamber and the microfluidic channel, and a freeze-dried reaction reagent is provided in the reagent chamber.

4. The portable nucleic acid detection device according to claim 1, characterized in that: A heating element for heating the reaction chamber is arranged outside the reaction chamber.

5. The portable nucleic acid detection device according to claim 1, characterized in that: A waste liquid chamber is also provided in the functional part, the waste liquid chamber is communicated with the microfluidic flow channel, and the waste liquid chamber is communicated with the lower chamber through an exhaust channel.

6. The portable nucleic acid detection device according to claim 1, characterized in that: A sealing member is provided on the puncture member. When the functional member is in the lower position, the sealing member is spaced apart from the functional member. When the functional member is in the upper position, the sealing member abuts against the functional member to seal the upper chamber and the microfluidic channel.

7. The portable nucleic acid detection device according to claim 1, characterized in that: The functional part is provided with a push block, the outer shell is provided with an escape opening, and the push block is exposed from the escape opening.

8. The portable nucleic acid detection device according to claim 7, characterized in that: A stopper is detachably fitted in the avoidance opening. When the stopper is fitted in the avoidance opening, the push block is stopped to limit the upward movement of the functional part. A pinching structure is provided on the stopper.

9. The portable nucleic acid detection device according to claim 1, characterized in that: The microfluidic channel has an S-shaped anti-backflow section.

10. The portable nucleic acid detection device according to claim 1, characterized in that: A breathable and water-proof member is provided between the exhaust channel and the reaction chamber.

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