Rapid evaluation device for magnetization intensity of magnetic beads

Abstract

The utility model relates to a device for rapidly evaluating the magnetization intensity of a magnetic bead. Comprising a first clamping plate for placing a plurality of test tubes, a supporting plate arranged at the bottom of the first clamping plate, a second clamping plate which is in sliding connection with the top of the first clamping plate and is used for lifting the test tubes, and magnets which are arranged on two side walls of the supporting plate and are used for the same magnetic field distribution, the test tubes respectively comprise a test tube containing a magnetic bead magnetization intensity standard substance and a test tube containing a magnetic bead sample with magnetization intensity to be measured, and the test tubes can enter or leave the magnetic field along with the lifting of the second clamping plate. Compared with the prior art, the device has the advantages that the same magnetic field intensity and the same magnetization starting time are met through the design, the problems of high cost, long consumed time and the like are avoided, the problem of non-uniform standards under the condition that the magnetic attraction time is used as a measurement basis is also avoided, and the magnetic force intensity of the magnetic bead sample can be simply, conveniently and quickly determined; time and economic cost are saved, and the method has important significance in the field of biological magnetic bead materials.

Description

Technical Field

[0001] The utility model relates to the technical field of magnetic nano material detection equipment, in particular to a device for quickly evaluating the magnetization intensity of magnetic beads. Background Art

[0002] Magnetic nanomaterials refer to special materials with nanometer sizes and magnetic properties. Magnetic nanoparticles combine the advantages of both magnetic materials and nanomaterials. They have the characteristics of large specific surface area, strong magnetic responsiveness, good biocompatibility, superparamagnetism, and easy adsorption. They are widely used in life sciences, energy, environment and other fields. Among them, biomagnetic beads used in the biomedical field are the most representative.

[0003] Saturation magnetization is an important physical parameter that reflects the strength of magnetic force of magnetic beads. The conventional measuring instrument is the vibrating sample magnetometer, which has accurate test data, but faces problems such as expensive instruments, long test time, and high cost. Therefore, most biological magnetic bead products simplify this parameter to magnetic attraction time as a measure of magnetic strength. This measurement basis lacks a unified standard. For example, the magnetic strength of the magnet used, the distance from the magnet, the concentration and volume of the magnetic bead solution will affect the magnetic attraction time of the magnetic beads.

[0004] Therefore, there is an urgent need for a magnetization intensity detection device that can unify the measurement standards. Utility Model Content

[0005] The purpose of the utility model is to provide a device for quickly evaluating the magnetization intensity of magnetic beads in order to overcome the defects of the prior art.

[0006] The purpose of the utility model can be achieved through the following technical solutions:

[0007] The technical solution of the utility model is to provide a device for quickly evaluating the magnetization intensity of magnetic beads, comprising a first card plate for placing a plurality of test tubes, a support plate arranged at the bottom of the first card plate, a second card plate slidably connected to the top of the first card plate and used for lifting the test tubes, and magnets arranged on both side walls of the support plate and used for the same magnetic field distribution. The test tubes respectively include test tubes containing magnetic bead magnetization intensity standards and test tubes containing magnetic bead samples of to-be-tested magnetization intensity. As the second card plate is lifted or lowered, the test tubes can enter or leave the magnetic field.

[0008] In some specific embodiments, the first card plate is provided with a plurality of first insertion holes for inserting and limiting the test tubes, and the second card plate is provided with second insertion holes corresponding to the first insertion holes.

[0009] In some specific embodiments, a plurality of the first plug holes are symmetrically located on the first card plates on both sides of the support plate.

[0010] Preferably, two rows of first plug holes are provided on the first card plate, which are symmetrically located on both sides of the support plate.

[0011] In some specific embodiments, the distances between two adjacent first plug holes on the same side of the support plate are the same.

[0012] In some specific embodiments, there are a plurality of magnets, which are respectively arranged on the side walls of the support plate corresponding to the first insertion holes.

[0013] In some embodiments, the magnet is a permanent magnet.

[0014] In some specific embodiments, the magnetic bead magnetization intensity rapid assessment device further includes a base plate disposed at the bottom of the support plate.

[0015] In some specific embodiments, a positioning groove corresponding to the first socket is provided on the base plate.

[0016] In some specific embodiments, the second pallet is slidably connected to the top of the first pallet via a sliding assembly, and the sliding assembly includes a slide rail, a lifting bracket, and a pulley provided at the end of the lifting bracket and slidably connected to the slide rail.

[0017] In some specific embodiments, the lifting bracket includes a first lifting bracket and a second lifting bracket that are cross-connected, and a movable buckle that is arranged at the cross-connection and is used to adjust the angle between the first lifting bracket and the second lifting bracket.

[0018] Compared with the prior art, the utility model visualizes the concept of magnetization intensity by equipping a series of standard samples, uses the second card plate to disperse each magnetic bead sample away from the magnetic field, and simultaneously enters the magnetic field with the same magnetic field intensity, and can simply and quickly evaluate the magnetization intensity of the sample to be tested by simply comparing the adsorption of the magnetic beads of the sample to be tested and the magnetic beads of the standard sample. The same magnetic field intensity and the same magnetization start time are achieved by design, and the high cost and time-consuming problems of using a vibrating sample magnetometer are avoided without the need to accurately test the saturation magnetization intensity of the magnetic beads, and the problem of inconsistent standards when the magnetic absorption time is used as the basis for measurement is also avoided. The magnetic strength of the magnetic bead sample can be determined simply and quickly, saving time and economic costs, which is of great significance to the field of biological magnetic bead materials. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a structural schematic diagram of the utility model.

[0020] The figure shows the following:

[0021] 1 is a base plate, 2 is a support plate, 3 is a first clamping plate, 4 is a second clamping plate, 5 is a positioning groove, 6 is a first plug hole, 7 is a second plug hole, 8 is a magnet, 9 is a lifting bracket, 10 is a slide rail, 11 is a movable buckle, 12 is a pulley, 13 is a test tube containing a magnetic bead magnetization intensity standard, and 14 is a test tube containing a magnetic bead sample of a magnetization intensity to be measured. DETAILED DESCRIPTION

[0022] The present invention is described in detail below in conjunction with the accompanying drawings and specific embodiments. This embodiment is implemented based on the technical solution of the present invention, and provides a detailed implementation method and a specific operation process, but the protection scope of the present invention is not limited to the following embodiments.

[0023] It should be noted that similar reference numerals and letters denote similar items in the following drawings, and therefore, once an item is defined in one drawing, further definition and explanation thereof is not required in subsequent drawings.

[0024] In conjunction with the accompanying drawings, some embodiments of the present invention are described in detail below. In the absence of conflict, the following embodiments and features in the embodiments can be combined with each other.

[0025] In the following embodiments, unless otherwise specified, functional components or structures are conventional components or conventional structures used in the art to achieve corresponding functions.

[0026] Embodiment 1:

[0027] like Figure 1As shown, a magnetic bead magnetization intensity rapid evaluation device includes a first cardboard 3 for placing a plurality of test tubes, a support plate 2 arranged at the bottom of the first cardboard 3, a second cardboard 4 slidably connected to the top of the first cardboard 3 and used to lift the test tubes, a magnet 8 arranged on the two side walls of the support plate 2 and used for the same magnetic field distribution, and a base plate 1 arranged at the bottom of the support plate 2. The test tubes are test tubes 13 containing magnetic bead magnetization intensity standards and test tubes 14 containing magnetic bead samples of the magnetization intensity to be measured, respectively. As the second cardboard 4 is lifted and lowered, the test tubes can enter or leave the magnetic field. The first cardboard 3 is provided with a plurality of first jacks 6 for inserting and limiting the test tubes, the second cardboard 4 is provided with a second jack 7 corresponding to the first jack 6, and the base plate 1 is provided with a positioning groove 5 corresponding to the first jack 6. Preferably, the apertures of the first jack 6 and the second jack 7 are smaller than the apertures at the opening of the test tubes, so as to stably limit the test tubes. Several first jacks 6 are also symmetrically located on the first cardboard 3 on both sides of the support plate 2. Exemplarily, two rows of first jacks 6 are provided on the first cardboard 3, which are symmetrically located on both sides of the support plate 2, and the distance between two adjacent first jacks 6 on the same side of the support plate 2 is the same. Several magnets 8 are provided, which are respectively arranged on the side walls of the support plate 2 corresponding to the first jacks 6 to form a magnetic field of equal strength to adsorb the magnetic beads in the test tubes in the first jacks 6. The magnet 8 is a permanent magnet. Exemplarily, a permanent magnet with weaker magnetism is preferably used to prevent each standard product in the test tube 13 containing the magnetic bead magnetization strength standard product from being quickly and completely adsorbed under a stronger magnetic field without obvious time difference. The second clamping plate 4 is slidably connected to the top of the first clamping plate 3 through a sliding assembly. The sliding assembly includes a slide rail 10, a lifting bracket 9, and a pulley 12 provided at the end of the lifting bracket 9 and slidably connected to the slide rail 10, respectively provided on the same side of the first clamping plate 3 and the second clamping plate 4. The lifting bracket 9 includes a first lifting bracket and a second lifting bracket cross-connected, and a movable buckle 11 provided at the cross-connection and used to adjust the angle between the first lifting bracket and the second lifting bracket.When the end of the first lifting bracket slides in a direction away from the end of the second lifting bracket on the same horizontal line, and at the same time, the end of the second lifting bracket slides in a direction away from the end of the first lifting bracket on the same horizontal line, the second clamping plate 4 loaded with the test tube 13 containing the magnetic bead magnetization strength standard or the test tube 14 containing the magnetic bead sample with the magnetization strength to be measured can move downward to a position close to the magnet 8 on the lower support plate 2, so that the magnetic beads in the test tube are enriched on its side wall; when the end of the first lifting bracket slides in a direction close to the end of the second lifting bracket on the same horizontal line, and at the same time, the end of the second lifting bracket slides in a direction close to the end of the first lifting bracket on the same horizontal line, the second clamping plate 4 loaded with the test tube 13 containing the magnetic bead magnetization strength standard and the test tube 14 containing the magnetic bead sample with the magnetization strength to be measured can move upward away from the magnet 8, so that the magnetic beads in the test tube are redispersed in the aqueous solution.

[0028] The utility model uses a second card plate 4 for sliding and lifting provided on the first card plate 3 to achieve the uniform descent or rise of the test tube 13 containing the magnetic bead magnetization intensity standard and the test tube 14 containing the magnetic bead sample with the magnetization intensity to be measured, so that the magnetization time is kept consistent. In addition, the distance between the designed jack and the magnet 8 is consistent, so that the samples in the test tubes are subjected to the magnetic field of equal strength.

[0029] Therefore, it is only necessary to use a series of magnetic bead magnetization intensity standard samples and magnetic bead magnetization intensity samples to be tested with the same volume and solid content, take the standard samples as reference, use the second card plate 4 to make each test tube enter the magnetic field at the same time, and compare the adsorption time of the magnetic beads of the sample to be tested and the magnetic beads of the standard sample, so as to simply and quickly evaluate the magnetization intensity of the sample to be tested.

[0030] Exemplarily, this embodiment also provides a method for using the magnetic bead magnetization intensity rapid evaluation device based on the utility model:

[0031] Prepare 7 test tubes 13 containing standard magnetic bead solution samples, numbered ①-⑦, with saturation magnetization intensities of 10, 20, 30, 40, 50, 60, and 70emu / g, respectively. They are dispersed in pure water with a solid content of 2%. Each test tube contains 1mL of sample solution and is placed in the second jack 7 on the same side of the second card plate 4.

[0032] The magnetic bead sample of the magnetization to be measured is also dispersed in pure water with a solid content of 2%, and 1 mL is taken in a test tube and placed in the second jack 7 on the other side of the second card plate 4. The second card plate 4 is raised until the test tube completely leaves the magnet area, and the magnetic beads are shaken to be evenly dispersed in each test tube. Then the second card plate 4 is quickly lowered to ensure that the bottom of each test tube is located in the corresponding positioning groove 5 on the base plate 1 and enters the magnetic field at the same time. Observe the speed of magnetic bead adsorption in each test tube. If the magnetic beads in the test tube No. 4 are completely adsorbed and the magnetic beads in the test tube No. 5 are not completely adsorbed, if the magnetic beads to be measured in the test tube 14 containing the magnetic bead sample of the magnetization to be measured are completely adsorbed, the magnetization of the magnetic beads to be measured is within the range of 40 to 50emu / g.

[0033] In summary, the magnetic bead magnetization intensity rapid evaluation device provided by the utility model can quickly estimate the magnetic strength of the magnetic bead sample in a short time, and can improve the accuracy of the evaluation by changing the gradient of the standard sample, which is of great significance to the field of biological magnetic beads.

[0034] The above description of the embodiments is to facilitate the understanding and use of the utility model by those skilled in the art. It is obvious that those familiar with the art can easily make various modifications to these embodiments and apply the general principles described herein to other embodiments without creative work. Therefore, the utility model is not limited to the above embodiments, and improvements and modifications made by those skilled in the art based on the disclosure of the utility model without departing from the scope of the utility model should be within the scope of protection of the utility model.

Claims

1. A device for rapidly evaluating the magnetization intensity of magnetic beads, characterized in that: The invention comprises a first clamping plate (3) for placing a plurality of test tubes, a support plate (2) arranged at the bottom of the first clamping plate (3), a second clamping plate (4) slidably connected to the top of the first clamping plate (3) and used for lifting the test tubes, and magnets (8) arranged on the two side walls of the support plate (2) and used for the same magnetic field distribution. The test tubes respectively comprise a test tube (13) containing a magnetic bead magnetization intensity standard and a test tube (14) containing a magnetic bead sample of a magnetization intensity to be measured. As the second clamping plate (4) is lifted or lowered, the test tubes can enter or leave the magnetic field.

2. The magnetic bead magnetization intensity rapid evaluation device according to claim 1, characterized in that: The first clamping plate (3) is provided with a plurality of first insertion holes (6) for inserting and limiting the test tubes, and the second clamping plate (4) is provided with second insertion holes (7) corresponding to the first insertion holes (6).

3. The magnetic bead magnetization intensity rapid evaluation device according to claim 2, characterized in that: A plurality of the first insertion holes (6) are symmetrically located on the first clamping plate (3) on both sides of the support plate (2).

4. The magnetic bead magnetization intensity rapid evaluation device according to claim 3, characterized in that: The distance between two adjacent first insertion holes (6) located on the same side of the support plate (2) is the same.

5. The magnetic bead magnetization intensity rapid evaluation device according to claim 2, characterized in that: A plurality of magnets (8) are provided, which are respectively arranged on the side walls of the support plate (2) corresponding to the first insertion holes (6).

6. The device for rapid evaluation of magnetic bead magnetization intensity according to claim 5, characterized in that: The magnet (8) is a permanent magnet.

7. The device for rapid evaluation of magnetic bead magnetization intensity according to claim 2, characterized in that: The magnetic bead magnetization intensity rapid assessment device further comprises a base plate (1) arranged at the bottom of the support plate (2).

8. The device for rapid evaluation of magnetic bead magnetization intensity according to claim 7, characterized in that: The base plate (1) is provided with a positioning groove (5) corresponding to the first insertion hole (6).

9. The device for rapid evaluation of magnetic bead magnetization intensity according to claim 1, characterized in that: The second card plate (4) is slidably connected to the top of the first card plate (3) via a sliding assembly, and the sliding assembly comprises a slide rail (10) respectively arranged on the same side of the first card plate (3) and the second card plate (4), a lifting bracket (9), and a pulley (12) arranged at the end of the lifting bracket (9) and slidably connected to the slide rail (10).

10. The device for rapid evaluation of magnetic bead magnetization intensity according to claim 9, characterized in that: The lifting bracket (9) comprises a first lifting bracket and a second lifting bracket which are cross-connected, and a movable buckle (11) which is arranged at the cross-connection and is used to adjust the angle between the first lifting bracket and the second lifting bracket.

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