Anesthesia-free mouse upper genital tract microbial inoculation and lavage device

Abstract

The utility model relates to the technical field of mouse upper genital tract experiment devices, and particularly discloses an anesthesia-free mouse upper genital tract microorganism inoculation and lavage device which comprises a uterine cavity needle tube, a connecting base is arranged at the bottom of the uterine cavity needle tube, and an inoculation solution storage part is connected to the bottom of the connecting base. A sterile filter element is arranged at the bottom of the inoculation solution storage part, a pipettor connecting part is mounted at the bottom of the sterile filter element, and the uterine cavity needle tube is connected with an inoculator body part consisting of the connecting base, the inoculation solution storage part, the sterile filter element and the pipettor connecting part. Under the condition that anesthesia is not needed, after a mouse vaginal stent is adopted to expose a cervical orifice, an inoculator is placed to complete microbial inoculation of an upper genital tract or uterine cavity lavage, the device does not depend on anesthesia, a filter element is added, and after sterilization treatment, the situation that liquid flows back, cross contamination with a pipettor is caused, and the microbiological detection result is affected can be avoided. The microbiological detection accuracy is ensured.

Description

Technical Field

[0001] The utility model relates to the technical field of mouse upper reproductive tract experimental devices, in particular to an anesthesia-free mouse upper reproductive tract microorganism inoculation and irrigation device. Background Art

[0002] Laboratory mice are wild mice that have been selected and bred by humans for a long time. They have been used for animal experiments since the 18th century and are currently the most widely used, most clearly studied, and most in-depth experimental animals. There are more than 500 breeds and strains of mice that have been bred. When inoculating and irrigating the reproductive tract of mice with microorganisms, special inoculation and irrigating devices are used. However, existing devices for inoculating and irrigating the upper reproductive tract of mice with microorganisms usually rely on anesthesia when used, and inevitably have phenomena such as liquid reflux and cross-contamination with pipettes, which affect the results of microbial detection and lead to low accuracy of microbial detection. Utility Model Content

[0003] The utility model aims to provide an anesthesia-free mouse upper reproductive tract microorganism inoculation and irrigation device which can avoid liquid reflux.

[0004] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: an anesthesia-free mouse upper reproductive tract microbial inoculation and irrigation device, comprising a uterine cavity needle tube, a connecting base is provided at the bottom of the uterine cavity needle tube, the bottom of the connecting base is connected to an inoculation solution storage part, a sterile filter core is provided at the bottom of the inoculation solution storage part, a connecting pipette part is installed at the bottom of the sterile filter core, the uterine cavity needle tube is connected to an inoculator body composed of a connecting base, an inoculation solution storage part, a sterile filter core and a connecting pipette part, the uterine cavity needle tube, the connecting base, the inoculation solution storage part, the sterile filter core and the connecting pipette part form an inoculator, and the top of the inoculator is provided with a mouse vagina bracket.

[0005] As a preferred technical solution of the utility model, the tip opening of the intrauterine needle tube is tapered, the length of the intrauterine needle tube is 2.5-2.8 cm, the outer diameter of the intrauterine needle tube is 0.07-0.08 cm, and the inner diameter is 0.04-0.06 cm.

[0006] As a preferred technical solution of the utility model, the inoculation solution storage unit can store less than 100ul of solution.

[0007] As a preferred technical solution of the present utility model, the sum of the lengths of the connecting base and the inoculation solution storage portion is 2.0 cm.

[0008] As a preferred technical solution of the utility model, the outer diameter of the inoculation solution storage part is 0.34 cm and the length is 1.6~1.8 cm.

[0009] As a preferred technical solution of the present utility model, the pipette connection portion can be adapted to a 200ul pipette.

[0010] As a preferred technical solution of the utility model, the mouse vagina stent is made of polypropylene high-temperature sterilized material as a whole, with a length of 1.1 cm, an outer diameter of 0.4~0.42 cm, and an inner diameter of 0.35~0.36 cm.

[0011] Compared with the prior art, the utility model has the following beneficial effects:

[0012] The utility model uses a mouse vaginal stent to expose the cervical opening, and then inserts an inoculator to complete the upper reproductive tract microbial inoculation or uterine cavity lavage without anesthesia. This device does not rely on anesthesia, and a filter element is added. After sterilization, it can avoid liquid reflux and cross contamination with the pipette, ensuring accurate microbial detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0014] Figure 2 This is a schematic diagram of the overall structure of the inoculator of the utility model;

[0015] Figure 3 The utility model is a schematic diagram of the structure of the mouse vagina stent.

[0016] In the figure: 1. Intrauterine needle tube; 2. Connecting base; 3. Inoculation solution storage part; 4. Sterile filter element; 5. Connecting part of pipette; 6. Mouse vaginal stent; 8. Partially enlarged schematic diagram of the tip of the intrauterine needle tube. DETAILED DESCRIPTION

[0017] The present application will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the relevant utility model, rather than to limit the utility model.

[0018] See also Figure 1-3 A device for inoculating and irrigating the upper reproductive tract of mice without anesthesia comprises a uterine cavity needle tube 1, a connecting base 2 is arranged at the bottom of the uterine cavity needle tube 1, an inoculation solution storage part 3 is connected to the bottom of the connecting base 2, a sterile filter core 4 is arranged at the bottom of the inoculation solution storage part 3, a connecting pipette part 5 is installed at the bottom of the sterile filter core 4, the uterine cavity needle tube 1 is connected with an inoculator body composed of the connecting base 2, the inoculation solution storage part 3, the sterile filter core 4 and the connecting pipette part 5, the uterine cavity needle tube 1, the connecting base 2, the inoculation solution storage part 3, the sterile filter core 4 and the connecting pipette part 5 form an inoculator, the inoculator is made of high-temperature sterilization materials such as polypropylene as a whole, and a mouse vagina bracket 6 is sleeved on the top of the inoculator.

[0019] As a preferred technical solution of the utility model, the tip opening of the intrauterine needle tube 1 is tapered, the length of the intrauterine needle tube 1 is 2.5~2.8cm, the outer diameter of the intrauterine needle tube 1 is 0.07~0.08cm, and the inner diameter is 0.04~0.06cm. The inoculation solution storage part 3 can store solutions below 100ul, the sum of the lengths of the connecting base 2 and the inoculation solution storage part 3 is 2.0cm, the outer diameter of the inoculation solution storage part 3 is 0.34cm, and the length is 1.6~1.8cm, the connecting pipette part 5 can be adapted to a 200ul pipette, and the mouse vagina stent 6 is made of high-temperature sterilizable materials such as polypropylene, with a length of 1.1cm, an outer diameter of 0.4~0.42cm, and an inner diameter of 0.35~0.36cm. It can be seen that the utility model uses a mouse vaginal stent 6 to expose the cervical opening without anesthesia, and then inserts an inoculator to complete upper reproductive tract microbial inoculation or uterine cavity lavage. This device does not rely on anesthesia, and a filter element is added. After sterilization, it can avoid liquid reflux and cross contamination with the pipette, which can affect the microbial detection results, thereby ensuring accurate microbial detection.

[0020] The working principle and use process of the utility model are as follows: first, the inoculator and the mouse vaginal stent 6 need to be used in combination, the pipette is connected to the inoculator, the microbial solution is quantitatively aspirated, the mouse is held in one hand with the abdomen facing upward, the reproductive tract is exposed, and the vaginal stent 2 is inserted to the bottom of the vagina to expose the cervical opening without anesthesia, the inoculator 1 is inserted, and the bacterial solution is injected. The uterine cavity irrigation method is the same as this process. The device is simple and easy to use, reduces the risk and pain of anesthesia for mice, can accurately place the microbial solution into the upper reproductive tract, and the sterile and filter element design avoids the interference of cross contamination on subsequent microbial detection.

[0021] Therefore, the utility model can solve the problem of the existing mouse upper reproductive tract microbial inoculation and irrigation device proposed in the above background technology. When in use, this equipment usually relies on anesthesia, and it is inevitable that liquid reflux and cross-contamination with the pipette will affect the microbial detection results, resulting in low accuracy of microbial detection.

[0022] The above description is only a preferred embodiment of the present application and an explanation of the technical principles used. Those skilled in the art should understand that the scope of the utility model involved in the present application is not limited to the technical solution formed by a specific combination of the above technical features.

Claims

1. A device for inoculating and lavaging the upper reproductive tract of mice without anesthesia, comprising a uterine cavity needle tube (1), characterized in that: The bottom of the intrauterine needle tube (1) is provided with a connecting base (2), the bottom of the connecting base (2) is connected to an inoculation solution storage part (3), the bottom of the inoculation solution storage part (3) is provided with a sterile filter core (4), the bottom of the sterile filter core (4) is installed with a connecting pipette part (5), the intrauterine needle tube (1) is connected with an inoculator body composed of the connecting base (2), the inoculation solution storage part (3), the sterile filter core (4) and the connecting pipette part (5), the intrauterine needle tube (1), the connecting base (2), the inoculation solution storage part (3), the sterile filter core (4) and the connecting pipette part (5) form an inoculator, and the top of the inoculator is provided with a mouse vagina bracket (6).

2. The device for inoculating and irrigating the upper reproductive tract of mice without anesthesia according to claim 1, characterized in that: The tip of the intrauterine needle tube (1) is tapered, the length of the intrauterine needle tube (1) is 2.5-2.8 cm, the outer diameter of the intrauterine needle tube (1) is 0.07-0.08 cm, and the inner diameter is 0.04-0.06 cm.

3. The device for inoculating and irrigating the upper reproductive tract of mice without anesthesia according to claim 1, characterized in that: The inoculation solution storage part (3) can store less than 100 ul of solution.

4. The device for inoculating and irrigating the upper reproductive tract of mice without anesthesia according to claim 1, characterized in that: The sum of the lengths of the connecting base (2) and the inoculation solution storage portion (3) is 2.0 cm.

5. The device for inoculating and irrigating the upper reproductive tract of mice without anesthesia according to claim 1, characterized in that: The inoculation solution storage portion (3) has an outer diameter of 0.34 cm and a length of 1.6-1.8 cm.

6. The device for inoculating and irrigating the upper reproductive tract of mice without anesthesia according to claim 1, characterized in that: The pipette connection part (5) can be adapted to a 200ul pipette.

7. The device for inoculating and irrigating the upper reproductive tract of mice without anesthesia according to claim 1, characterized in that: The mouse vagina stent (6) is made entirely of polypropylene high-temperature sterilizable material, has a length of 1.1 cm, an outer diameter of 0.4-0.42 cm, and an inner diameter of 0.35-0.36 cm.

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