SiPM-based fluorescent confocal microscopic imaging detector

Abstract

The utility model relates to the technical field of bridges, in particular to a SiPM-based fluorescent confocal microscopic imaging detector, which comprises a detector body, a light source, a light emitting diode (LED) light source, a light emitting diode (LED) light source and a light emitting diode (LED) light source, a mounting panel fixedly connected with the detector body, wherein the mounting panel is provided with a terminal interface, a bias power supply interface and a signal processing output port; the signal amplifier substrate is fixedly connected in the detector body, and a signal amplifier and a signal processing module which are connected with each other are integrated on the signal amplifier substrate; and the SiPM substrate is fixedly connected in the detector body, a SiPM module connected with the signal amplifier is integrated on the SiPM substrate, a photosensitive area is arranged on the SiPM module, and the photosensitive area and the detection interface are coaxially arranged. According to the utility model, the SiPM module is used as a core component, and the detection capability under a low-light condition is greatly improved by using the SiPM module, so that weak signals can be effectively captured and amplified.

Description

Technical Field

[0001] The utility model relates to the technical field of medical imaging equipment, in particular to a fluorescence confocal microscopic imaging detector based on SiPM. Background Art

[0002] The laser confocal microscope uses a laser beam as the light source. The laser beam passes through the illumination pinhole, is reflected to the objective lens through the beam splitter, and is focused on the sample to scan every point on the focal plane of the specimen. If there is a fluorescent substance that can be excited in the tissue sample, the fluorescence emitted after being excited directly returns to the beam splitter through the original incident light path, and is first focused when passing through the detection pinhole. The focused light is collected by the photomultiplier tube (PMT) detector, and the signal is transmitted to the computer. After processing, the image is displayed on the computer monitor.

[0003] Therefore, the detector is the most important device for laser confocal microscopy imaging. Traditional PMT-based fluorescence detectors have the following defects: 1. PMT may generate high noise during operation, reducing the signal-to-noise ratio and affecting the image quality; 2. Although PMT has high sensitivity within a certain range, its dynamic range is limited and it is prone to signal saturation or distortion, especially when processing strong and weak light signals, which limits the image quality and affects the image clarity; 3. The existing PMT detector structure is too complex and not easy to maintain. Utility Model Content

[0004] In order to solve the above problems in the prior art, the utility model provides a fluorescence confocal microscopy imaging detector based on SiPM, which uses the SiPM module as the core component and combines the characteristics of high sensitivity and high efficiency, which helps to improve the detection capability under low light conditions and capture weak signals, thereby enhancing the sensitivity and resolution of the system. At the same time, the integrated amplifier module has the ability to adjust the amplification factor and gain, so that the system can expand the dynamic range and process strong and weak light signals at the same time, thereby improving the adaptability and flexibility of the imaging system.

[0005] In order to achieve the above-mentioned purpose, the main technical solutions adopted by the utility model include:

[0006] A detector body, wherein the detector is provided with a detection interface;

[0007] A mounting panel fixedly connected to the detector body, wherein the mounting panel is provided with a terminal interface, a bias power interface and a signal processing output port;

[0008] A signal amplifier substrate fixedly connected to the detector body, the signal amplifier substrate integrating a signal amplifier and a signal processing module connected to each other, the signal processing module connected to the signal processing output port to realize the output after signal processing, and the signal amplifier substrate externally connected to a regulated power supply through the terminal interface;

[0009] A SiPM substrate is fixedly connected to the detector body, and a SiPM module connected to the signal amplifier is integrated on the SiPM substrate. A photosensitive area is provided on the SiPM module, and the photosensitive area is coaxially arranged with the detection interface. The SiPM module is connected to an external regulated power supply through the bias power supply interface to form a bias current.

[0010] Furthermore, the signal amplifier is a transimpedance signal amplifier. The transimpedance signal amplifier has a bandwidth speed better than 50MHz, is powered by a positive or negative 5V DC voltage, can amplify the photocurrent signal into an analog voltage signal, Uout = Ipd * Rs, the transimpedance resistance Rs = 10kΩ, and the output voltage range is 0-3V, which can maximize the performance of the SiPM module.

[0011] Furthermore, the signal amplifier substrate is also provided with an amplifier signal output port connected to the signal processing module and an amplifier signal receiving port connected to the signal amplifier, the amplifier signal output port is connected to the signal processing output port, the SiPM substrate is also provided with a SiPM output port and a SiPM power input port connected to the bias power interface, and the amplifier signal receiving port is connected to the SiPM output port. In this way, standardized port connections between various modules are achieved, which facilitates the connection of various modules.

[0012] Furthermore, the bias power interface, signal processing output port, amplifier signal output port, amplifier signal receiving port, SiPM output port and SiPM power input port are all SMA interfaces. The SMA interface is used to implement the standardized interface of each module, so that modules from different manufacturers can be compatible with each other, and users can more easily customize and build SiPM systems suitable for specific applications, thereby improving the flexibility and adaptability of the system.

[0013] Furthermore, the bias power interface is externally connected to a 28V DC power supply, and the bias current of the detector is formed by the external 28V DC power supply.

[0014] Furthermore, the terminal interface is an XH1.53-3PIN terminal interface, and the terminal interface is externally connected to a ±5V DC power supply, which makes modules from different manufacturers compatible with each other and more practical.

[0015] Furthermore, the area size of the photosensitive area is 6*6MM, so that adding the SiPM module can more effectively capture photon signals and increase the range of receiving light signals.

[0016] Furthermore, the inner wall of the detector body forms a raised mounting platform, and the mounting platform is provided with an assembly through hole for connecting the signal amplifier substrate and the SiPM substrate. The signal amplifier substrate and the SiPM substrate can be installed by setting the mounting platform, making the installation more stable and not easy to fall off.

[0017] Furthermore, the mounting platform is provided with a protective groove connected to the detection interface.

[0018] Furthermore, an external SMA interface is integrated on the SiPM substrate, which can facilitate the SiPM to be externally connected to other modules that need to be coordinated, and the functional scalability is better.

[0019] Compared with the prior art, the beneficial effects of the utility model are as follows:

[0020] 1. Improve sensitivity and resolution: The SiPM module is used as the core component. The use of the SiPM module greatly improves the detection capability under low light conditions, so that weak signals can also be effectively captured and amplified. The integrated signal amplifier can effectively amplify the weak light signal generated by the SiPM module, thereby improving the sensitivity of the system. This is very important for fluorescence imaging under low light conditions, and can capture more weak signals and improve imaging quality.

[0021] 2. Optimize the signal-to-noise ratio: The ratio between signal and noise is critical to imaging quality. The utility model integrates a signal amplifier to effectively amplify the weak light signal generated by the SiPM module while reducing the noise level of the system, which helps to improve the signal-to-noise ratio, making the image clearer and the signal easier to detect and analyze. The integrated signal amplifier in the utility model has the ability to adjust the amplification factor and gain, enabling the system to expand the dynamic range, process strong and weak light signals at the same time, and improve the adaptability and flexibility of the imaging system.

[0022] 3. Reduce costs and improve maintainability: Traditional imaging systems are usually costly and complex to maintain. The utility model adopts a modular design, allowing different components to be designed and produced independently, reducing the development and production costs of the system. At the same time, the modular design simplifies the maintenance and repair of the system. When a module fails or needs to be upgraded, only the specific module needs to be replaced, reducing the maintenance cost and maintenance time, and improving the reliability and stability of the system.

[0023] 4. Improve customization and standardized interfaces: The modular design in this utility model enables users to select, replace or upgrade specific modules according to specific application requirements without changing the entire system. At the same time, the modular design is accompanied by the adoption of standardized interfaces, making modules from different manufacturers compatible with each other, and users can more easily customize and build SiPM systems suitable for specific applications, improving the flexibility and adaptability of the system. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 This is a schematic diagram of the structure of the SiPM-based fluorescence confocal microscopic imaging detector of the present invention;

[0025] Figure 2 for Figure 1 Explosion diagram of

[0026] Figure 3 It is a schematic diagram of a detector body of the utility model from one perspective;

[0027] Figure 4 This is a schematic diagram of the structural principle of the utility model;

[0028] Figure 5 This is a schematic diagram of the principle of the signal amplifier.

[0029] [Description of Reference Numerals]

[0030] 1. Detector body; 11. Detection interface; 12. Mounting table; 13. Protection groove; 14. Assembly through hole; 2. Mounting panel; 21. Terminal interface; 22. Bias power interface; 23. Signal processing output port; 3. Signal amplifier substrate; 31. Signal amplifier; 32. Signal processing module; 33. Amplifier signal output port; 34. Amplifier signal receiving port; 4. SiPM substrate; 41. SiPM module; 42. Photosensitive area; 43. SiPM output port; 44. SiPM power input port; 45. External SMA interface. DETAILED DESCRIPTION

[0031] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0032] It should be noted that all directional indications in the embodiments of the present invention (such as up, down, left, right, front, back...) are only used to explain the relative position relationship, movement status, etc. between the components under a certain specific posture (as shown in the accompanying drawings). If the specific posture changes, the directional indication will also change accordingly.

[0033] In the present invention, unless otherwise clearly specified and limited, the terms "connection", "fixation", etc. should be understood in a broad sense. For example, "fixation" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly defined. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0034] In addition, if there are descriptions involving "first", "second", etc. in the embodiments of the utility model, the descriptions of "first", "second", etc. are only used for descriptive purposes and cannot be understood as indicating or implying their relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In addition, the meaning of "and / or" appearing in the full text includes three parallel schemes. Taking "A and / or B" as an example, it includes scheme A, or scheme B, or a scheme that satisfies both A and B. In addition, the technical solutions between the various embodiments can be combined with each other, but it must be based on the ability of ordinary technicians in this field to implement. When the combination of technical solutions is contradictory or cannot be implemented, it should be deemed that such a combination of technical solutions does not exist and is not within the scope of protection required by the utility model.

[0035] like Figures 1 to 5 As shown, this embodiment provides a fluorescence confocal microscopic imaging detector based on SiPM, including:

[0036] like Figure 1 As shown, the detector body 1 is provided with a detection interface 11;

[0037] like Figure 2 As shown, a mounting panel 2 is fixedly connected to the detector body 1, and a terminal interface 21, a bias power interface 22 and a signal processing output port 23 are provided on the mounting panel 2;

[0038] A signal amplifier substrate 3 is fixedly connected to the detector body 1, and a signal amplifier 31 and a signal processing module 32 are integrated on the signal amplifier substrate 3. In particular, the signal amplifier 31 of the present embodiment is a transimpedance signal amplifier, and the signal processing module 32 is connected to the signal processing output port 23 to realize the output after signal processing. Specifically, the signal amplifier substrate 3 is also provided with an amplifier signal output port 33 connected to the signal processing module 32 and an amplifier signal receiving port 34 connected to the signal amplifier 31, and the amplifier signal output port 33 is connected to the signal processing output port 23, so that the processed electrical signal can be transmitted to an external processor through the signal processing output port 23 for storage and subsequent analysis.

[0039] The signal amplifier substrate 3 is connected to an external voltage-stabilized power supply through the terminal interface 21. The terminal interface 21 is an XH1.53-3PIN terminal interface. The terminal interface 21 is connected to an external ±5V DC power supply. The transimpedance signal amplifier used in this way has a bandwidth speed better than 50MHz. It is powered by positive and negative 5V DC voltage and can amplify the photocurrent signal of the SiPM module 41 into an analog voltage signal. The conversion formula is: Uout = Ipd*Rs, transimpedance resistance Rs = 10kΩ, and the output voltage range is 0-3V;

[0040] A SiPM substrate 4 is fixedly connected to the detector body 1, and a SiPM module 41 connected to the signal amplifier 31 is integrated on the SiPM substrate 4. A photosensitive area 42 is provided on the SiPM module 41, and the area of ​​the photosensitive area 42 is 6*6MM. The photosensitive area 42 is coaxially arranged with the detection interface 11. The SiPM module 41 is connected to an external regulated power supply through the bias power supply interface 22 to form a bias current. The bias power supply interface 22 is externally connected to a 28V DC power supply. Specifically, a SiPM output port 43 and a SiPM power input port 44 connected to the bias power supply interface 22 are also provided on the SiPM substrate 4, and the amplifier signal receiving port 34 is connected to the SiPM output port 43.

[0041] In order to realize standardized interfaces between modules, the bias power interface 22, signal processing output port 23, amplifier signal output port 33, amplifier signal receiving port 34, SiPM output port 43 and SiPM power input port 44 of this embodiment are all SMA interfaces.

[0042] In order to facilitate the installation of the signal amplifier substrate 3 and the SiPM substrate 4, a raised mounting platform 12 is formed on the inner wall of the detector body 1, and an assembly through hole 14 for connecting the signal amplifier substrate 3 and the SiPM substrate 4 is provided on the mounting platform 12. The signal amplifier 31 and the SiPM can be sequentially mounted on the mounting platform 12 by matching the assembly through hole 14 with bolts or the like.

[0043] In this embodiment, if Figure 3 Therefore, a protective groove 13 connected to the detection interface 11 is provided on the mounting platform 12. When the SiPM substrate 4 is mounted on the mounting platform 12, the photosensitive area 42 will be completely immersed in the protective groove 13. At this time, it can be avoided that the photosensitive area 42 can only receive the light signal from the detection interface 11 and will not be affected by the light from other positions, thereby confirming the accuracy of its work.

[0044] In this embodiment, an external SMA interface 45 is also integrated on the SiPM substrate 4, which can facilitate the SiPM to be externally connected to other modules that need to be coordinated, and the functional expandability is better.

[0045] The working principle of the present invention is briefly described as follows: Taking the present invention installed on a fluorescence confocal microscope as an example, the present invention replaces the existing PET detector and is installed on a precisely placed focal plane of the microscope so that the photosensitive area 42 is aligned with the pinhole. When the laser system of the microscope cooperates with each group of lenses to excite the fluorescent material of the sample on the stage, emitting fluorescence in all directions and transmitting it to the photosensitive area 42, the photosensitive area 42 receives the fluorescence signal and converts it into an electrical signal. The SiPM output port 43 and the amplifier signal output port 33 are connected to send the electrical signal to the signal amplifier 31. After the signal amplifier 31 amplifies the received electrical signal, the signal processing module 32 processes the electrical signal to obtain data that can be processed by the microscope. The signal processing output port 23 is connected to the processing system of the microscope and the processed data is transmitted to the processing system for collection and storage.

[0046] The above-mentioned embodiments only express several implementation methods of the utility model, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the patent of the utility model. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the utility model, and these all belong to the protection scope of the utility model. Therefore, the protection scope of the utility model patent shall be based on the attached claims.

Claims

1. A fluorescence confocal microscopy detector based on SiPM, characterized in that: include: A detector body (1), wherein a detection interface (11) is provided on the detector; A mounting panel (2) fixedly connected to the detector body (1), wherein the mounting panel (2) is provided with a terminal interface (21), a bias power interface (22) and a signal processing output port (23); A signal amplifier substrate (3) fixedly connected to the detector body (1), the signal amplifier substrate (3) integrating mutually connected signal amplifiers (31) and signal processing modules (32), the signal processing module (32) being connected to the signal processing output port (23) to realize output after signal processing, and the signal amplifier substrate (3) being externally connected to a regulated power supply via the terminal interface (21); A SiPM substrate (4) fixedly connected to the detector body (1), the SiPM substrate (4) having an integrated SiPM module (41) connected to the signal amplifier (31), the SiPM module (41) having a photosensitive area (42), the photosensitive area (42) being coaxially arranged with the detection interface (11), and the SiPM module (41) being connected to an external regulated power supply via the bias power supply interface (22) to form a bias current.

2. A SiPM-based fluorescence confocal microscopy imaging detector according to claim 1, characterized in that: The signal amplifier (31) is a transimpedance signal amplifier.

3. The SiPM-based fluorescence confocal microscopy imaging detector according to claim 1, characterized in that: The signal amplifier substrate (3) is also provided with an amplifier signal output port (33) connected to the signal processing module (32) and an amplifier signal receiving port (34) connected to the signal amplifier (31); the amplifier signal output port (33) is connected to the signal processing output port (23); the SiPM substrate (4) is also provided with a SiPM output port (43) and a SiPM power input port (44) connected to the bias power interface (22); the amplifier signal receiving port (34) is connected to the SiPM output port (43).

4. A SiPM-based fluorescence confocal microscopy imaging detector according to claim 3, characterized in that: The bias power supply interface (22), the signal processing output port (23), the amplifier signal output port (33), the amplifier signal receiving port (34), the SiPM output port (43) and the SiPM power supply input port (44) are all SMA interfaces.

5. The SiPM-based fluorescence confocal microscopy imaging detector according to claim 1, characterized in that: The bias power supply interface (22) is externally connected to a 28V DC power supply.

6. The SiPM-based fluorescence confocal microscopy imaging detector according to claim 1, characterized in that: The terminal interface (21) is an XH1.53-3PIN terminal interface, and the terminal interface (21) is externally connected to a ±5V direct current power supply.

7. The SiPM-based fluorescence confocal microscopy imaging detector according to claim 1, characterized in that: The area size of the photosensitive area (42) is 6*6MM.

8. The SiPM-based fluorescence confocal microscopy imaging detector according to claim 1, characterized in that: A protruding mounting platform (12) is formed on the inner wall of the detector body (1), and an assembly through hole (14) for connecting a signal amplifier substrate (3) and a SiPM substrate (4) is provided on the mounting platform (12).

9. The SiPM-based fluorescence confocal microscopy imaging detector according to claim 8, characterized in that: The mounting platform (12) is provided with a protection groove (13) which is in communication with the detection interface (11).

10. The SiPM-based fluorescence confocal microscopy imaging detector according to claim 1, characterized in that: An external SMA interface (45) is also integrated on the SiPM substrate (4).

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