Animal in-vitro intestinal tract analogue simulation device

Abstract

The utility model relates to the technical field of in-vitro intestinal tract simulation, in particular to an animal in-vitro intestinal tract analogue simulation device which comprises a main body container, a plurality of intestinal tract simulation units are arranged in the main body container, and a feeding system, a circulating system and a control system are further arranged in the main body container. The feeding system, the circulating system and the control system are electrically connected with the intestinal tract simulation unit. The digestion process in the animal intestinal tract can be simulated more truly through the arrangement of the multiple intestinal tract simulation units, and each unit can independently simulate different digestion stages. Due to the integrated design of the feeding system, the circulating system and the control system, the flowing of food in the intestinal tract, the action of digestive enzymes and the intestinal tract movement can be effectively simulated. By arranging the wriggling control piece and the contraction and relaxation piece, wriggling movement in the intestinal tract can be simulated, and the authenticity of simulation is enhanced.

Description

Technical Field

[0001] The utility model relates to the technical field of in vitro intestinal simulation, in particular to an in vitro intestinal simulation device for animals. Background Art

[0002] With the deepening of biomedical research, the understanding and research of animal digestion process has become increasingly important. Traditional animal digestion experiments often need to be carried out in animals, which is not only costly but also may cause harm to animals. Therefore, the development of a device that can simulate the intestinal digestion process of animals in vitro is of great significance for reducing experimental costs, improving experimental efficiency and animal welfare. Utility Model Content

[0003] In view of this, the present invention aims to solve the problem that traditional animal digestion experiments often need to be conducted in animals, which is not only costly but also may cause harm to animals.

[0004] The utility model provides an in vitro intestinal simulation device for animals, comprising: a main body container, a plurality of intestinal simulation units are arranged inside the main body container, and a feeding system, a circulation system and a control system are also arranged inside the main body container, and the feeding system, the circulation system and the control system are all electrically connected to the intestinal simulation units.

[0005] Optionally, a plurality of the intestinal simulation units form an intestinal simulation system, a feeding system is provided at the intestinal simulation unit closest to the top of the main container, and a control system and a circulation system are provided at the intestinal simulation unit farthest from the top of the main container.

[0006] Optionally, each of the intestinal simulation units is connected to the control system and the circulatory system, and the circulatory system includes a peristaltic control component and a contraction and relaxation component. The peristaltic control component is arranged around each of the intestinal simulation units, and the contraction and relaxation component is spaced apart from the peristaltic control component. A contraction and relaxation component is arranged between every two peristaltic control components, and a peristaltic control component is arranged between every two contraction and relaxation components.

[0007] Optionally, the control system includes a digestive enzyme control component, a temperature controller and a pH controller, the digestive enzyme control component is individually connected to each of the intestinal simulation units, and the temperature controller and the pH controller are connected to the intestinal simulation system.

[0008] Optionally, each intestinal simulation unit is also provided with a temperature sensor, a pH sensor and a temperature regulator, the temperature sensor is electrically connected to the temperature controller, the temperature regulator is electrically connected to the temperature controller, and the pH sensor is electrically connected to the pH controller.

[0009] Optionally, the digestive enzyme control component further includes a digestive enzyme control tank and a digestive enzyme controller, wherein the digestive enzyme control tank is individually connected to each of the intestinal simulation units, and the digestive enzyme control tank is also electrically connected to the digestive enzyme controller.

[0010] Optionally, a plurality of storage bins are provided in the digestive enzyme control tank, and different digestive enzymes are contained in the storage bins, including protease, pancreatic amylase, pancreatic lipase, aminopeptidase, α-dextrinase, maltase, peptidase and oligopeptidase.

[0011] Optionally, the pH controller also includes a pH adjustment chamber, which is connected to the intestinal simulation system. A plurality of storage components are arranged in the pH adjustment chamber, and different buffer solutions are contained in the storage components. The buffer solutions include hydrochloric acid, phosphate buffer, sodium hydroxide and sodium bicarbonate.

[0012] Compared with the prior art, the in vitro intestinal simulation device provided by the utility model has the following beneficial effects: the in vitro intestinal simulation device provided by the utility model can simulate the digestion process in the intestinal tract of animals more realistically through the setting of multiple intestinal simulation units, and each unit can independently simulate different digestion stages. The integrated design of the feeding system, the circulation system and the control system can effectively simulate the flow of food in the intestine, the action of digestive enzymes and intestinal peristalsis. Through the setting of peristaltic control parts and contraction and relaxation parts, peristaltic movement in the intestine can be simulated to enhance the authenticity of the simulation. The digestive enzyme control parts, temperature controllers and pH controllers in the control system can accurately control each intestinal simulation unit respectively to ensure the accuracy and repeatability of the experiment. The setting of the temperature sensor, pH sensor and temperature regulator can monitor and adjust the temperature and pH value in the intestinal simulation unit in real time, and better simulate the intestinal environment. The setting of the digestive enzyme control tank can provide a variety of digestive enzymes to simulate the digestion process of different types of food in the intestine. The setting of the pH adjustment chamber can provide a variety of buffer solutions to help maintain the pH stability in the intestinal simulation unit. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 An overall schematic diagram of an in vitro intestinal simulation device for an animal provided by an embodiment of the utility model;

[0014] Figure 2This is a schematic diagram of the connection between the pH adjustment chamber and the intestinal simulation system of an in vitro intestinal simulation device for an animal provided by an embodiment of the utility model;

[0015] Figure 3 A schematic diagram of the connection between a digestive enzyme control tank and a plurality of intestinal simulation units of an in vitro intestinal simulation device for an animal provided by an embodiment of the utility model;

[0016] Among them: 101, feeding system; 102, intestinal simulation system; 103, control system; 104, main container; 105, peristaltic control component; 106, contraction and relaxation component; 107, pH adjustment chamber; 108, temperature regulator; 109, digestive enzyme control tank; 110, intestinal simulation unit. DETAILED DESCRIPTION

[0017] The following will be combined with the drawings in the embodiments of the present application to clearly and completely describe the technical solutions in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of this application.

[0018] In the description of the present application, it should be understood that the terms "center", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present application.

[0019] The terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of the features. In the description of this application, unless otherwise specified, "plurality" means two or more.

[0020] In the description of this application, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood according to specific circumstances.

[0021] The utility model provides an in vitro intestinal simulation device for animals. Figure 1-3 It can be seen that the present application includes: a main container 104, a plurality of intestinal simulation units 110 are arranged inside the main container 104, and a feeding system 101, a circulation system and a control system 103 are also arranged inside the main container 104, and the feeding system 101, the circulation system and the control system 103 are all electrically connected to the intestinal simulation unit 110.

[0022] In this preferred embodiment, the multiple intestinal simulation units 110 form an intestinal simulation system 102, and the intestinal simulation unit 110 closest to the top of the main container 104 is provided with a feeding system 101, and the intestinal simulation unit 110 far from the top of the main container 104 is provided with a control system 103 and a circulation system.

[0023] In this preferred embodiment, each of the intestinal simulation units 110 is connected to the control system 103 and the circulatory system, and the circulatory system includes a peristaltic control component 105 and a contraction and relaxation component 106. The peristaltic control component 105 is arranged around each of the intestinal simulation units 110, and the contraction and relaxation component 106 is spaced apart from the peristaltic control component 105. A contraction and relaxation component 106 is arranged between every two peristaltic control components 105, and a peristaltic control component 105 is arranged between every two contraction and relaxation components 106.

[0024] In this preferred embodiment, the control system 103 includes a digestive enzyme control unit, a temperature controller and a pH controller. The digestive enzyme control unit is individually connected to each of the intestinal simulation units 110 , and the temperature controller and the pH controller are connected to the intestinal simulation system 102 .

[0025] It is understandable that the digestion process in the animal intestine can be simulated more realistically by setting up multiple intestinal simulation units 110, and each unit can independently simulate different digestion stages. The integrated design of the feeding system 101, the circulation system and the control system 103 can effectively simulate the flow of food in the intestine, the action of digestive enzymes and intestinal peristalsis. The peristaltic control member 105 and the contraction and relaxation member 106 can be set to simulate the peristaltic movement in the intestine and enhance the authenticity of the simulation.

[0026] In this preferred embodiment, each intestinal simulation unit 110 is also provided with a temperature sensor, a pH sensor and a temperature regulator 108, the temperature sensor is electrically connected to the temperature controller, the temperature regulator 108 is electrically connected to the temperature controller, and the pH sensor is electrically connected to the pH controller.

[0027] In this preferred embodiment, the digestive enzyme control unit also includes a digestive enzyme control tank 109 and a digestive enzyme controller. The digestive enzyme control tank 109 is individually connected to each of the intestinal simulation units 110, and the digestive enzyme control tank 109 is also electrically connected to the digestive enzyme controller.

[0028] It is understandable that the digestive enzyme control unit, temperature controller and pH controller in the control system 103 can respectively accurately control each intestinal simulation unit 110 to ensure the accuracy and repeatability of the experiment. The setting of the temperature sensor, pH sensor and temperature regulator 108 can monitor and adjust the temperature and pH value in the intestinal simulation unit 110 in real time to better simulate the intestinal environment. The setting of the digestive enzyme control tank 109 can provide a variety of digestive enzymes to simulate the digestion process of different types of food in the intestine. The setting of the pH adjustment chamber 107 can provide a variety of buffer solutions to help maintain the pH stability in the intestinal simulation unit 110.

[0029] In this preferred embodiment, the digestive enzyme control tank 109 is provided with a plurality of storage bins, and different digestive enzymes are contained in the storage bins, including protease, pancreatic amylase, pancreatic lipase, aminopeptidase, α-dextrinase, maltase, peptidase and oligopeptidase.

[0030] As you can understand, the small intestine can be divided into three parts: the duodenum, jejunum, and ileum. Food enters the duodenum from the stomach and begins the digestion process in the small intestine.

[0031] Duodenum: This is the first part of the small intestine where food from the stomach mixes with pancreatic juice secreted by the pancreas. Pancreatic juice contains a variety of enzymes, such as trypsin, amylase, and lipase, which are used to break down proteins, carbohydrates, and fats, respectively. In addition, the duodenal wall also secretes enterokinase, an enzyme that activates trypsinogen to become trypsin, which begins the digestion of proteins.

[0032] Jejunum: This is the middle section of the small intestine where food continues to mix with pancreatic juice and intestinal juice. Intestinal juice is mainly secreted by glands in the jejunum and ileum and contains a variety of enzymes such as aminopeptidase, α-dextrinase and maltase, which further break down proteins and carbohydrates.

[0033] Ileum: This is the last part of the small intestine and its main function is to absorb nutrients, such as vitamin B12 and fatty acids, as well as absorb water and electrolytes. Enzyme activity in the ileum is relatively low, but there are still some enzymes involved in the final digestive process, such as peptidases and oligopeptidases, which help break down remaining proteins.

[0034] Therefore, the present application selects different storage bins to store digestive enzymes so that they can play a role in different intestinal simulation units 110, which is more in line with the actual situation.

[0035] In this preferred embodiment, the pH controller also includes a pH adjusting chamber 107, and the pH adjusting chamber 107 is connected to the intestinal simulation system 102. A plurality of storage components are arranged in the pH adjusting chamber 107, and different buffer solutions are contained in the storage components. The buffer solutions include hydrochloric acid, phosphate buffer, sodium hydroxide and sodium bicarbonate.

[0036] It is understandable that the above buffer solutions all have their own uses:

[0037] Hydrochloric acid: can be used to lower the pH and simulate the situation of stomach acid entering the duodenum.

[0038] Phosphate Buffer: Phosphate buffer can be used to maintain a specific pH value as it is able to resist changes in pH over a certain range.

[0039] Sodium hydroxide: can be used to raise the pH, especially when simulating the conditions of the jejunum and ileum.

[0040] Sodium bicarbonate: Since bicarbonate secreted by the pancreas in the small intestine can help neutralize stomach acid, sodium bicarbonate can also be used to adjust pH when simulating the small intestine environment.

[0041] An in vitro intestinal simulation device of an animal in each of the above embodiments can simulate the digestion process in the intestinal tract of an animal more realistically by setting up multiple intestinal simulation units, and each unit can independently simulate different digestion stages. The integrated design of the feeding system, the circulation system and the control system can effectively simulate the flow of food in the intestine, the action of digestive enzymes and intestinal peristalsis. Through the setting of peristaltic control parts and contraction and relaxation parts, peristaltic movement in the intestine can be simulated to enhance the authenticity of the simulation. The digestive enzyme control parts, temperature controllers and pH controllers in the control system can accurately control each intestinal simulation unit respectively to ensure the accuracy and repeatability of the experiment. The setting of the temperature sensor, pH sensor and temperature regulator can monitor and adjust the temperature and pH value in the intestinal simulation unit in real time, and better simulate the intestinal environment. The setting of the digestive enzyme control tank can provide a variety of digestive enzymes to simulate the digestion process of different types of food in the intestine. The setting of the pH adjustment chamber can provide a variety of buffer solutions to help maintain the pH stability in the intestinal simulation unit.

[0042] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.

Claims

1. An in vitro intestinal simulation device for animals, characterized in that: include: A main container is provided with a plurality of intestinal simulation units inside the main container, and a feeding system, a circulation system and a control system are also provided inside the main container, and the feeding system, the circulation system and the control system are all electrically connected to the intestinal simulation units.

2. The in vitro intestinal simulation device of an animal according to claim 1, characterized in that: The multiple intestinal simulation units constitute an intestinal simulation system. A feeding system is arranged at the intestinal simulation unit closest to the top of the main container, and a control system and a circulation system are arranged at the intestinal simulation unit far from the top of the main container.

3. The in vitro intestinal simulation device of an animal according to claim 2, characterized in that: Each of the intestinal simulation units is connected to the control system and the circulatory system. The circulatory system includes a peristaltic control component and a contraction and relaxation component. The peristaltic control component is arranged around each of the intestinal simulation units. The contraction and relaxation component and the peristaltic control component are arranged at intervals. A contraction and relaxation component is arranged between every two peristaltic control components, and a peristaltic control component is arranged between every two contraction and relaxation components.

4. The in vitro intestinal simulation device of an animal according to claim 2, characterized in that: The control system includes a digestive enzyme control component, a temperature controller and a pH controller. The digestive enzyme control component is individually connected to each of the intestinal simulation units, and the temperature controller and the pH controller are connected to the intestinal simulation system.

5. The in vitro intestinal simulation device of an animal according to claim 4, characterized in that: Each intestinal simulation unit is also provided with a temperature sensor, a pH sensor and a temperature regulator. The temperature sensor is electrically connected to the temperature controller, the temperature regulator is electrically connected to the temperature controller, and the pH sensor is electrically connected to the pH controller.

6. The in vitro intestinal tract simulation device of an animal according to claim 5, characterized in that: The digestive enzyme control component also includes a digestive enzyme control tank and a digestive enzyme controller. The digestive enzyme control tank is individually connected to each of the intestinal simulation units, and the digestive enzyme control tank is also electrically connected to the digestive enzyme controller.

7. The in vitro intestinal simulation device of an animal according to claim 6, characterized in that: The digestive enzyme control tank is provided with a plurality of storage bins, and different digestive enzymes are contained in the storage bins. The digestive enzymes include protease, pancreatic amylase, pancreatic lipase, aminopeptidase, α-dextrinase, maltase, peptidase and oligopeptidase.

8. The in vitro intestinal tract simulation device of claim 5, characterized in that: The pH controller also includes a pH adjustment chamber, which is connected to the intestinal simulation system. A plurality of storage components are arranged in the pH adjustment chamber, and different buffer solutions are contained in the storage components. The buffer solutions include hydrochloric acid, phosphate buffer, sodium hydroxide and sodium bicarbonate.

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