Placenta tissue grinding and inoculating device

Abstract

The utility model provides a placenta tissue grinding and inoculating device, which relates to the field of placenta grinding, and comprises a container bottle for placing placenta tissues; the grinding shell is rotationally connected to the open end of the container bottle, and a cavity of the grinding shell communicates with a cavity of the container bottle; a first cutter body and a second cutter body are arranged in the grinding shell, the first cutter body and the second cutter body rotate relatively, and a grinding space is formed between the first cutter body and the second cutter body; an adjusting piece for adjusting the size of the cut placenta tissue is arranged in the grinding shell, and the adjusting piece is located on the side, away from the container bottle, of the second cutter body; the inoculation part is detachably connected to the end, away from the container bottle, of the grinding shell, and a cavity of the inoculation part communicates with a cavity of the grinding shell; a culture dish convenient for inoculation is arranged in the inoculation part; the push rod assembly is used for pushing the ground tissue into the culture dish. The method has the effects of reducing the risk of high pollution and enabling the size of the ground tissue to be controllable.

Description

Technical Field

[0001] The present application relates to the field of placenta grinding, and in particular to a placenta tissue grinding and inoculation device. Background Art

[0002] With the growth and development of science and technology, people's understanding of stem cells has become deeper and deeper, and their understanding of the different types of stem cells contained in tissues has also become deeper and deeper. Typically, the clinical research and application of placental stem cells have become more and more extensive.

[0003] In the existing technology, manual inoculation is widely used in physical stem cell extraction technology, which not only has a high risk of contamination and low inoculation efficiency, but also easily leads to the disadvantage of uncontrollable size of the inoculated tissue. Utility Model Content

[0004] In order to reduce the risk of high contamination and make the size of ground tissue controllable, the present application provides a placenta tissue grinding and inoculation device.

[0005] To solve the above problems, the technical solution of the utility model is:

[0006] A placenta tissue grinding and inoculation device, comprising:

[0007] Container bottle for placing placenta-like tissue;

[0008] A grinding shell is rotatably connected to one end of the container bottle opening, and the chamber of the grinding shell is communicated with the chamber of the container bottle; a first blade body and a second blade body are arranged in the grinding shell, the first blade body is arranged around the second blade body, and the first blade body and the second blade body rotate relatively, and a grinding space is formed between the first blade body and the second blade body; an adjusting member for adjusting the size of the cut placenta tissue is arranged in the grinding shell, and the adjusting member is located on the side of the second blade body away from the container bottle;

[0009] An inoculation part is detachably connected to an end of the grinding shell away from the container bottle, and the chamber of the inoculation part is connected to the chamber of the grinding shell; a culture dish for inoculation is arranged in the inoculation part;

[0010] A push rod assembly, wherein the fixing portion is arranged on the container bottle, and the pushing portion passes through the container bottle and is located in the inner cavity of the grinding shell. The push rod assembly pushes the ground tissue into the culture dish.

[0011] The utility model relates to a placenta tissue grinding and inoculation device, wherein a support member is provided at one end of the grinding shell facing the container bottle, a support rod is fixed on the support member, and the support rod is located in the chamber of the grinding shell; the second blade is provided on the support rod, and the first blade is fixed on the inner wall of the grinding shell.

[0012] The utility model relates to a placenta tissue grinding and inoculation device, wherein a convex ring is arranged at the opening of the container bottle, and a groove ring is arranged on the end of the grinding shell facing the container bottle, wherein the convex ring is located in the groove ring and realizes the relative rotation of the container bottle and the grinding shell.

[0013] In the placenta tissue grinding and inoculation device of the utility model, a support spring is arranged on the support rod, and the support spring is located between the support member and the second knife body; wherein the second knife body is slidably connected to the support rod, and the diameter of the second knife body gradually increases from the container bottle toward the inoculation part;

[0014] A connecting rod is arranged on one end of the support rod away from the support member, the adjusting member is a spiral steel knife and is threadedly connected to the connecting rod, and the spiral steel knife pushes the second knife body away from or close to the support member.

[0015] The utility model provides a placenta tissue grinding and inoculation device, wherein the second knife body comprises a first reverse spiral steel knife and a second reverse spiral steel knife.

[0016] The placenta tissue grinding and inoculation device of the utility model is provided with a mounting channel on the container bottle, one end of the mounting channel is arranged on the bottom wall of the container bottle away from the opening, and the other end of the mounting channel is located at the opening of the container bottle; and both ends of the mounting channel are connected; the support rod and the connecting rod are provided with transition channels, and the two transition channels are connected;

[0017] The push rod assembly includes an extrusion housing, a fixing member, a push spring, an extension rod and a push plate;

[0018] The extrusion shell is detachably connected to the outer side wall of the bottom wall of the container bottle, the fixing piece is arranged on the inner wall of the extrusion shell, one end of the push spring is fixed to the fixing piece, and the other end of the push spring is fixed to the extension rod, the extension rod is located in the installation channel and extends to the two transition channels, and the push plate is arranged on the end of the extension rod away from the push spring, and the push plate is located on the side of the adjustment member away from the second blade body.

[0019] The placenta tissue grinding and inoculation device of the utility model comprises a first filter and a second filter; a plurality of first filter holes are provided on the bottom wall of the first filter, the opening end of the culture dish is detachably connected to the bottom inner wall of the second filter, and a plurality of second filter holes are provided on the bottom wall of the culture dish;

[0020] The first filter is threadedly connected to an end of the grinding shell away from the container bottle, the second filter is threadedly connected to the first filter, and the bottom wall of the first filter and the bottom wall of the culture dish abut against each other;

[0021] The first filter holes and the second filter holes are in a staggered state or a connected state.

[0022] The placenta tissue grinding and inoculation device of the utility model, the support member comprises an annular sheet and a plurality of support sheets, one end of the support sheet is arranged on the inner wall of the grinding shell, and the other end of the support sheet is fixed to the annular sheet;

[0023] Wherein, the end of the mounting channel facing the grinding shell abuts against the annular sheet, and the outer side wall of the support rod is fixed to the inner side wall of the annular sheet.

[0024] In the placenta tissue grinding and inoculation device of the utility model, the extrusion shell is connected to the end of the container bottle away from the grinding shell through a thread;

[0025] The push plate is threadedly connected to the extension rod.

[0026] In the placenta tissue grinding and inoculation device of the utility model, the cross section of the push plate is arc-shaped, and the push plate protrudes toward the direction of the culture dish.

[0027] Due to the adoption of the above technical solution, the utility model has the following advantages and positive effects compared with the prior art:

[0028] In this solution, the placenta tissue is first placed in a container bottle, the inoculation device is assembled, and then the container bottle is inverted to allow the placenta tissue in the container bottle to enter the grinding shell, that is, enter the grinding space; the first blade body and the second blade body rotate relative to each other to achieve grinding of the placenta tissue, and the size of the grinding particles can be adjusted by a regulator, thereby achieving the characteristic of controllable size of the ground tissue;

[0029] After grinding is completed, the push rod assembly can push the tissue in the grinding shell into the culture dish, thereby reducing contamination of the tissue and improving the success rate of tissue culture. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1Schematic diagram of the overall structure of the placenta tissue grinding and inoculation device in the embodiment of the present application

[0031] Figure 2 Schematic diagram of the support structure of the placenta tissue grinding and inoculation device according to the embodiment of the present application

[0032] Figure 3 Schematic diagram of the structure of the convex ring and the groove ring of the placenta tissue grinding and inoculation device according to the embodiment of the present application

[0033] Explanation of the accompanying drawings: 1. container bottle; 2. grinding shell; 3. first knife body; 4. second knife body; 5. adjusting member; 6. culture dish; 7. supporting member; 8. supporting rod; 9. convex ring; 10. groove ring; 11. supporting spring; 12. connecting rod; 13. mounting channel; 14. transition channel; 15. extrusion shell; 16. fixing member; 17. pushing spring; 18. extension rod; 19. pushing plate; 20. first filter; 21. second filter; 22. first filter hole; 23. second filter hole; 24. annular sheet; 25. supporting sheet. DETAILED DESCRIPTION

[0034] The advantages and features of the present invention will be more clearly understood from the following description and claims.

[0035] See also Figures 1 to 3 The present embodiment provides a placenta tissue grinding and inoculation device, comprising a container bottle 1, a grinding shell 2, an inoculation part and a push rod assembly; the container bottle 1 is used to place placenta tissue; the grinding shell 2 is rotatably connected to one end of the container bottle 1, and the chamber of the grinding shell 2 is communicated with the chamber of the container bottle 1; a first knife body 3 and a second knife body 4 are arranged in the grinding shell 2, the first knife body 3 is arranged around the second knife body 4 and the first knife body 3 and the second knife body 4 rotate relatively, and a grinding space is formed between the first knife body 3 and the second knife body 4; an adjusting member 5 for adjusting the size of the cut placenta tissue is arranged in the grinding shell 2, and the adjusting member 5 is located on the side of the second knife body 4 away from the container bottle 1; the inoculation part is detachably connected to one end of the grinding shell 2 away from the container bottle 1, and the chamber of the inoculation part is communicated with the chamber of the grinding shell 2; a culture dish 6 for easy inoculation is arranged in the inoculation part; the fixed part of the push rod assembly is arranged on the container bottle 1, the pushing part passes through the container bottle 1 and is located in the inner cavity of the grinding shell 2, and the push rod assembly pushes the ground tissue into the culture dish 6.

[0036] It is further provided that the axis of the container bottle 1 and the axis of the grinding shell 2 are coaxially arranged.

[0037] In this solution, the placenta tissue is first placed in the container bottle 1, the inoculation device is assembled, and then the container bottle 1 is inverted to allow the placenta tissue in the container bottle 1 to enter the grinding shell 2, that is, enter the grinding space; the first blade body 3 and the second blade body 4 rotate relative to each other to achieve the grinding of the placenta tissue, and the size of the grinding particles can be adjusted by the adjustment member 5, thereby achieving the characteristic of controllable size of the ground tissue;

[0038] After grinding is completed, the push rod assembly can push the tissue in the grinding shell 2 into the culture dish 6, thereby reducing contamination of the tissue and improving the success rate of tissue culture.

[0039] The specific structure of the placenta tissue grinding and inoculation device of this embodiment is further described below:

[0040] In this embodiment, a support member 7 is provided at one end of the grinding shell 2 facing the container bottle 1, a support rod 8 is fixed on the support member 7, and the support rod 8 is located in the cavity of the grinding shell 2; the second blade body 4 is provided on the support rod 8, and the first blade body 3 is fixed on the inner wall of the grinding shell 2.

[0041] It is further arranged that the axis of the support rod 8 and the axis of the container bottle 1 are coaxially arranged; wherein the support rod 8 is in the shape of a rectangular parallelepiped, a matching groove is provided on the second blade body 4, and the rectangular parallelepiped is located in the matching groove, so that the inner wall of the matching groove fits the outer wall of the rectangular parallelepiped.

[0042] In this embodiment, a convex ring 9 is provided at the opening of the container bottle 1, and a groove ring 10 is provided on the end of the grinding shell 2 facing the container bottle 1. The convex ring 9 is located in the groove ring 10 and realizes the relative rotation of the container bottle 1 and the grinding shell 2.

[0043] It is further configured that the convex ring 9 on the container bottle 1 can be arranged on the inner wall or outer wall of the container bottle 1, and the corresponding groove ring 10 on the grinding shell 2 can be arranged on the outer wall or inner wall of the grinding shell 2; the axis of the convex ring 9 and the groove ring 10 is also the same as the axis of the container bottle 1.

[0044] To further explain, the selected placenta tissue is cut into blocks and placed in the container bottle 1, the device is inverted, and the operator holds the container bottle 1 with one hand and the grinding shell 2 with the other hand. Through the connection method of the convex ring 9 and the groove ring 10, the grinding shell 2 can be rotated with each other, which prompts the first knife body 3 to rotate, and finally the placenta tissue is ground.

[0045] In this embodiment, a support spring 11 is provided on the support rod 8, and the support spring 11 is located between the support member 7 and the second blade body 4; wherein the second blade body 4 is slidably connected to the support rod 8, and the diameter of the second blade body gradually increases from the container bottle toward the inoculation part;

[0046] A connecting rod 12 is provided on one end of the support rod 8 away from the support member 7 . The adjusting member 5 is a spiral steel knife and is threadedly connected to the connecting rod 12 . The spiral steel knife pushes the second knife body 4 away from or close to the support member 7 .

[0047] Further explanation, during the installation process of the adjusting member 5 (the grinding shell 2 is located above the container bottle 1), the adjusting member 5 moves downward through the thread, causing the adjusting member 5 to approach the container bottle 1. During this process, the supporting spring 11 can abut against the second blade body 4 to cause the second blade body 4 to move stably; since the diameter of the second blade body is different at different positions, the distance between it and the first blade body 3 is also different, and then the distance between the first blade body 3 and the second blade body 4 can be adjusted by the regulator, thereby realizing the adjustment of the size of the grinding tissue. Among them, the distance refers to the distance between the second blade body 4 and the first blade body 3 in the diameter direction.

[0048] In this embodiment, the second blade body 4 includes a first reverse spiral steel blade and a second reverse spiral steel blade.

[0049] Further, the first reverse spiral steel blade and the second reverse spiral steel blade are arranged to realize two grinding processes, so that the grinding is more refined.

[0050] In this embodiment, the container bottle 1 is provided with a mounting channel 13, one end of the mounting channel 13 is provided on the bottom wall of the container bottle 1 away from the opening, and the other end of the mounting channel 13 is located at the opening of the container bottle 1. Moreover, both ends of the mounting channel 13 are connected. To further explain, the mounting channel 13 and the container bottle 1 are formed as one body.

[0051] The support rod 8 and the connecting rod 12 are both provided with transition passages 14 , and the two transition passages 14 are connected.

[0052] The push rod assembly includes an extrusion housing 15, a fixing member 16, a push spring 17, an extension rod 18 and a push plate 19; the extrusion housing 15 is detachably connected to the outer wall of the bottom surface of the container bottle 1, and the fixing member 16 is arranged on the inner wall of the extrusion housing 15 to increase the hardness and thickness. One end of the push spring 17 is fixed to the fixing member 16, and the other end of the push spring 17 is fixed to the extension rod 18, and the extension rod 18 is located in the installation channel 13 and extends to the two transition channels 14. The end of the extension rod 18 away from the push spring 17 is provided with a push plate 19, and the push plate 19 is located on the side of the adjustment member 5 away from the second blade 4.

[0053] Further, the extrusion shell 15 is made of rubber material. At this time, pushing the extrusion shell 15 causes the extrusion shell 15 to deform, thereby driving the fixing member 16, the pushing spring 17, and the extension rod 18 to move, and the push rod moves toward the culture dish 6, thereby pushing the ground tissue into the culture dish 6;

[0054] After the pushing is completed, the push spring 17 is pressed against the deformation formed on the outer wall of the container bottle 1 to achieve recovery, and then pushes the extrusion shell 15 in the reverse direction to make the extrusion shell 15 recover.

[0055] In this embodiment, the inoculation part includes a first filter 20 and a second filter 21; a plurality of first filter holes 22 are formed on the bottom wall of the first filter 20, the open end of the culture dish 6 is detachably connected to the bottom inner wall of the second filter 21, and a plurality of second filter holes 23 are formed on the bottom wall of the culture dish 6;

[0056] The first filter 20 is threadedly connected to the end of the grinding shell 2 away from the container bottle 1, and the second filter 21 is threadedly connected to the first filter 20, and the bottom wall of the first filter 20 and the bottom wall of the culture dish 6 are in contact with each other;

[0057] The first filter holes 22 and the second filter holes 23 are in a staggered state or a connected state.

[0058] It is further provided that the culture dish 6 can be mounted upside down on the second filter 21 by means of a snap-fitting manner.

[0059] To further explain, when the operator rotates the grinding shell 2 for grinding, the inoculation part is located below the grinding shell 2, and the first filter hole 22 and the second filter hole 23 are connected, so that the ground tissue can smoothly enter the culture dish 6; after the grinding is completed, the first filter hole 22 and the second filter hole 23 are staggered, and the inoculation part is inverted to the top of the grinding shell 2. At this time, the tissue pushed into the culture dish 6 by the push plate 19 is placed on the bottom wall of the culture dish 6; then, the second filter 21 is removed from the grinding shell 2, and the culture dish 6 is removed from the second filter 21 by tweezers, and finally the culture dish 6 is placed in the experimental device.

[0060] It is further explained that, during the removal process, the existence of the second filter hole 23 may cause the ground tissue to fall off, so the second filter hole 23 needs to be set as small as possible within a reasonable range.

[0061] In this embodiment, the support member 7 includes an annular sheet 24 and a plurality of support sheets 25, one end of the support sheet 25 is fixed to the inner wall of the grinding shell 2, and the other end of the support sheet 25 is fixed to the annular sheet 24;

[0062] The end of the installation channel 13 facing the grinding shell 2 abuts against the annular sheet 24 , and the outer wall of the support rod 8 is fixed to the inner wall of the annular sheet 24 .

[0063] It is further provided that the inner cavity of the extrusion shell 15, the mounting channel 13, the support sheet 25 and the support rod 8 form a closed space, thereby reducing the entry of tissue into the closed space and reducing contamination.

[0064] In this embodiment, the extrusion housing 15 is connected to the end of the container bottle 1 away from the grinding housing 2 through a thread; and the push plate 19 is connected to the extension rod 18 through a thread.

[0065] To further illustrate the installation process: the extrusion shell 15, the fixing part 16, the push spring, and the extension rod 18 can be fixedly arranged, the container bottle 1 places the extension rod 18 therein through the installation channel 13, and the container bottle 1 and the extrusion shell 15 are threadedly connected; the grinding shell 2 is installed, the extension rod 18 passes through the two transition channels 14, and then the convex ring 9 is arranged in the groove ring 10; the second knife body 4, the regulator, and the push plate 19 are installed; the first filter 20 is threadedly connected to the grinding shell 2, and the second filter 21 with the culture dish 6 is threadedly connected to the first filter 20.

[0066] In this embodiment, the cross section of the push plate 19 is arc-shaped, and the push plate 19 protrudes toward the culture dish 6 .

[0067] The above is a detailed description of the embodiments of the present invention in conjunction with the accompanying drawings, but the present invention is not limited to the above embodiments. Even if various changes are made to the present invention, if these changes fall within the scope of the claims of the present invention and their equivalents, they still fall within the protection scope of the present invention.

Claims

1. A placenta tissue grinding and inoculation device, characterized in that: include, Container bottle for placing placenta-like tissue; A grinding shell is rotatably connected to one end of the container bottle opening, and the chamber of the grinding shell is communicated with the chamber of the container bottle; a first blade body and a second blade body are arranged in the grinding shell, the first blade body is arranged around the second blade body, and the first blade body and the second blade body rotate relatively, and a grinding space is formed between the first blade body and the second blade body; an adjusting member for adjusting the size of the cut placenta tissue is arranged in the grinding shell, and the adjusting member is located on the side of the second blade body away from the container bottle; An inoculation part is detachably connected to an end of the grinding shell away from the container bottle, and the chamber of the inoculation part is connected to the chamber of the grinding shell; a culture dish for inoculation is arranged in the inoculation part; A push rod assembly, wherein the fixing portion is arranged on the container bottle, and the pushing portion passes through the container bottle and is located in the inner cavity of the grinding shell. The push rod assembly pushes the ground tissue into the culture dish.

2. The placenta tissue grinding and inoculation device according to claim 1, characterized in that: A support member is provided at one end of the grinding shell facing the container bottle, a support rod is fixed on the support member, and the support rod is located in the cavity of the grinding shell; the second knife body is provided on the support rod, and the first knife body is fixed on the inner wall of the grinding shell.

3. The placenta tissue grinding and inoculation device according to claim 2, characterized in that: A convex ring is arranged at the opening of the container bottle, and a groove ring is arranged on the end of the grinding shell facing the container bottle. The convex ring is located in the groove ring and realizes the relative rotation of the container bottle and the grinding shell.

4. The placenta tissue grinding and inoculation device according to claim 3, characterized in that: A support spring is provided on the support rod, and the support spring is located between the support member and the second knife body; wherein the second knife body is slidably connected to the support rod, and the diameter of the second knife body gradually increases from the container bottle to the inoculation part; A connecting rod is arranged on one end of the support rod away from the support member, the adjusting member is a spiral steel knife and is threadedly connected to the connecting rod, and the spiral steel knife pushes the second knife body away from or close to the support member.

5. The placenta tissue grinding and inoculation device according to claim 4, characterized in that: The second blade body includes a first reverse spiral steel blade and a second reverse spiral steel blade.

6. The placenta tissue grinding and inoculation device according to claim 4, characterized in that: The container bottle is provided with a mounting channel, one end of which is provided on the bottom wall of the container bottle away from the opening, and the other end of which is located at the opening of the container bottle; and both ends of the mounting channel are connected; the support rod and the connecting rod are both provided with transition channels, and the two transition channels are connected; The push rod assembly includes an extrusion housing, a fixing member, a push spring, an extension rod and a push plate; The extrusion shell is detachably connected to the bottom outer wall of the container bottle, the fixing piece is arranged on the inner wall of the extrusion shell, one end of the push spring is fixed to the fixing piece, and the other end of the push spring is fixed to the extension rod, the extension rod is located in the installation channel and extends to the two transition channels, and the push plate is arranged on the end of the extension rod away from the push spring, and the push plate is located on the side of the adjustment member away from the second blade body.

7. The placenta tissue grinding and inoculation device according to claim 1, characterized in that: The inoculation part includes a first filter and a second filter; a plurality of first filter holes are provided on the bottom wall of the first filter, the opening end of the culture dish is detachably connected to the bottom inner wall of the second filter, and a plurality of second filter holes are provided on the bottom wall of the culture dish; The first filter is threadedly connected to an end of the grinding shell away from the container bottle, the second filter is threadedly connected to the first filter, and the bottom wall of the first filter and the bottom wall of the culture dish abut against each other; The first filter holes and the second filter holes are in a staggered state or a connected state.

8. The placenta tissue grinding and inoculation device according to claim 6, characterized in that: The support member includes an annular sheet and a plurality of support sheets, one end of the support sheet is arranged on the inner wall of the grinding shell, and the other end of the support sheet is fixed to the annular sheet; Wherein, the end of the mounting channel facing the grinding shell abuts against the annular sheet, and the outer side wall of the support rod is fixed to the inner side wall of the annular sheet.

9. The placenta tissue grinding and inoculation device according to claim 6, characterized in that: The extrusion shell is connected to the end of the container bottle away from the grinding shell through a thread; The push plate is threadedly connected to the extension rod.

10. The placenta tissue grinding and inoculation device according to claim 6, characterized in that: The cross section of the push plate is arc-shaped, and the push plate protrudes toward the culture dish.

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