Detection device for solving interference among bioluminescence holes

Abstract

The utility model relates to the technical field of biological detection, in particular to a detection device for solving interference among bioluminescence holes. According to the detection device, the problem of inter-hole interference during bioluminescence detection by using a transparent bottom hole plate is solved, an optical isolation area is formed for a single hole by using a film material laminating process and a mold hot pressing or mechanical pressing mode, and the reliability of detection data is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of biological detection, in particular to a detection device for solving the interference between biological luminescent holes. Background Art

[0002] Bioluminescence is a luminescence produced by natural enzymatic chemical reactions. It exists in many organisms, such as fireflies, deep-sea jellyfish, and some bacteria. In this reaction system, luciferin releases photons through oxidation under the catalysis of luciferase and ions. There is a certain relationship between the luminescence intensity produced by the reaction and the amount consumed, so the content can be determined by light intensity detection. Bioluminescence detection has high specificity, high sensitivity, and a wide measurement range. It is widely used in food hygiene, environmental monitoring, drug screening, medical research and other fields.

[0003] The main method of current bioluminescence detection is to read the light intensity value of the well plate through an optical detection device. In order to avoid optical interference between wells (when there is a luminous object in an adjacent well, the light in the adjacent well will pass through the well plate wall or bottom and enter the current measurement well, interfering with the reading of the current measurement well and causing errors in the measurement value), an opaque well plate is generally used. However, in some cases, a transparent bottom well plate must be used, which often leads to interference errors between wells. In order to solve the problem of interference between wells when using a transparent bottom well plate for bioluminescence experiments, it is of great practical significance to provide a detection device that enhances the performance of bioluminescence detection. Utility Model Content

[0004] In view of this, the purpose of the present invention is to overcome the defects in the existing detection technology and provide a detection device that solves the interference between bioluminescent wells. More specifically, a detection device that solves the interference between wells when using a transparent bottom well plate for bioluminescent detection is provided.

[0005] In order to achieve the above-mentioned utility model purpose, the utility model provides the following technical solutions:

[0006] The utility model provides a detection device for solving the interference between bioluminescent holes, comprising a component provided with a plurality of detection units; a metal or metal-plated material film is provided at the bottom of the detection unit.

[0007] In some specific embodiments of the present invention, the metal or metal-plated material film includes one or more of copper foil, aluminum foil, gold foil, tin foil or plastic reflective film.

[0008] In some specific embodiments of the present invention, the back side of the metal or metal-plated material film includes an adhesive material, and the metal or metal-plated material film is tightly combined with the bottom of the detection unit through the adhesive material.

[0009] In some specific embodiments of the present invention, a metal or metal-plated material film is provided at the bottom of the detection unit, and the metal or metal-plated material film is tightly combined with the bottom of the detection unit by lamination or hot pressing or mechanical processing.

[0010] In some specific embodiments of the present invention, bonding refers to using the metal or metal-plated material film to completely bond the bottom plane of the detection unit without leaving any bubbles or gaps.

[0011] In some specific embodiments of the present invention, the hot pressing includes using a mold to heat so that the metal or metal-plated material film is embedded in the bottom of the detection unit.

[0012] In some specific embodiments of the present invention, the temperature of the hot pressing is 110° C. and the pressure is 50N.

[0013] In some specific embodiments of the present invention, the mechanical processing includes processing the bottom of the detection unit into a groove, and using a mold to embed the metal or metal-plated material film into the bottom of the detection unit.

[0014] In some specific embodiments of the present invention, the side walls of the detection unit are also provided with a metal or metal-plated material film.

[0015] In some specific embodiments of the present invention, the component comprises a 6-well plate, a 12-well plate, a 24-well plate, a 48-well plate, a 96-well plate, a 384-well plate or a 1536-well plate, and the bottom of the component is transparent.

[0016] The detection device for solving the interference between bioluminescent wells provided by the utility model does not change the existing detection method of using optical detection equipment to read the well plate, and does not make complicated changes to the instruments and measuring devices. It only needs to stick a metal film and perform hot pressing to solve the interference problem between the wells of the transparent well plate on the existing instrument. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art are briefly introduced below.

[0018] Figure 1 The top schematic diagram of the overall structure is shown, wherein 1 is the orifice plate, 2 is the copper foil attached to the bottom of the orifice plate, and 3 is the hot pressing device;

[0019] Figure 2 The bottom schematic diagram of the overall structure is shown, wherein 1 is the orifice plate, 2 is the copper foil attached to the bottom of the orifice plate, and 3 is the hot pressing device. DETAILED DESCRIPTION

[0020] The utility model discloses a detection device for solving the interference between bioluminescent wells. Those skilled in the art can refer to the content of this article and appropriately improve the process parameters to achieve it. It is particularly important to point out that all similar replacements and modifications are obvious to those skilled in the art, and they are all considered to be included in the utility model. The method and application of the utility model have been described through preferred embodiments, and relevant personnel can obviously modify or appropriately change and combine the methods and applications described herein without departing from the content, spirit and scope of the utility model to implement and apply the technology of the utility model.

[0021] The utility model provides a detection device for solving the interference between bioluminescent wells. Metal material or metal-plated material film is attached to the bottom surface of the well plate to enhance the optical signal of bioluminescent and reduce the transmission of signal between wells, so as to reduce the interference between wells.

[0022] Based on the above-mentioned detection device, the utility model provides a solution to further solve the interference between bioluminescent holes. The metal or metal-plated material film is pressed into the area between the holes of the bottom plate by heat pressing or mechanical processing, so as to further reduce the transmission of optical signals between the holes, thereby reducing the interference between the holes, and obtaining a detection device that solves the interference between bioluminescent holes.

[0023] The utility model adopts the above technical solution, and compared with the prior art, has the following technical effects:

[0024] After using a bottom-bonded metal or metal-plated material film, the interference between holes can be reduced to less than 0.5%;

[0025] By bonding a metal or metal-plated film to the bottom and then using a hot pressing process, the interference between holes can be reduced to less than one ten-thousandth.

[0026] The utility model relates to a method for solving the interference between bioluminescent wells. Without changing the existing detection method of using optical detection equipment to read the well plate and without making complicated changes to the instrument and measuring device, only a method of pasting a metal film and performing hot pressing is added, thereby solving the problem of interference between the wells of the transparent well plate on the existing instrument.

[0027] As used herein, unless otherwise specified, the "transparent bottom well plate" refers to but is not limited to standard 6, 12, 24, 48, 96, 384, 1536 well plates with a transparent bottom.

[0028] As used herein, unless otherwise specified, the metal or metal-plated material film refers to but is not limited to copper foil, aluminum foil, gold foil, tin foil, plastic reflective film, etc. The metal or metal-plated material film has glue on the back and is suitable for bonding to the back of the orifice plate.

[0029] As used herein, unless otherwise specified, laminating the bottom of the well plate to the film means that the bottom plane of the well plate is completely adhered to the film material without leaving any bubbles or gaps.

[0030] As used herein, unless otherwise specified, hot pressing refers to but is not limited to using a mold to heat the membrane material to embed it into the bottom of the orifice plate, and using a mold to machine a groove in the bottom of the orifice plate and then embedding the membrane material into the bottom of the orifice plate.

[0031] The raw materials used in the detection device for solving the interference between bioluminescent holes provided by the utility model can all be purchased from the market.

[0032] The present invention will be further described below in conjunction with the embodiments:

[0033] The actual metal film used in the experiment is cut from ordinary copper foil tape, and the actual hot pressing device used is made of pure copper metal. A heating plate is installed at the bottom of the hot pressing device to control the temperature of the hot pressing plate at around 110 degrees Celsius.

[0034] The hot pressing process described in this article is to stick the metal film to the bottom surface of the orifice plate, heat the hot pressing device to about 110 degrees Celsius, place the bottom surface of the orifice plate on the hot pressing device, and apply a pressure of about 50 Newtons to the orifice plate to form the required optical isolation structure on the bottom surface of the orifice plate.

[0035] 1. Experimental materials, reagents and instruments

[0036] ATP standard (produced by Thermo Fisher Scientific), CTG (CellTiter-Glo cell viability reagent) reagent (produced by Promega), 96-well black bottom transparent well plate (produced by CORNING), microplate reader (produced by PerkinElmer)

[0037] 2. Experimental methods

[0038] a. Dilute the ATP standard into more than 3 gradient concentrations. The dilution for this test is 10 -8 , 10 -9 , 10 -10 mol / L;

[0039] b. Inject 100 μl of ATP standard of each concentration into non-adjacent positions of the 96-well plate;

[0040] c. Pour 100 μl of pure water into the 8 wells around the well containing the ATP standard;

[0041] d. Inject 100 μl of CTG reagent into the wells containing ATP reagent and pure water;

[0042] e. After standing for 10 minutes at 25 degrees Celsius, place the plate in the microplate reader and use the glow / chemiluminescence detection function to detect the luminescence values ​​of the reagent well and the surrounding 8 wells. Divide the average luminescence value of the 8 wells around the reagent well by the luminescence value of the reagent well to obtain the well-to-well interference rate of the untreated plate.

[0043] f. Paste the adhesive-backed copper foil on the bottom of the above-mentioned well plate. After pasting, use the glow detection function of the microplate reader to detect the luminescence value of the reagent well and the surrounding 8 wells. Divide the average luminescence value of the 8 wells around the reagent well by the luminescence value of the reagent well to obtain the inter-well interference rate of the well plate with the film material.

[0044] g. The copper foil hole plate with the bottom is hot pressed and embedded, and the luminescence value of the reagent hole and the surrounding 8 holes is detected by the glow detection function of the microplate reader. The average luminescence value of the 8 holes around the reagent hole is divided by the luminescence value of the reagent hole to obtain the inter-hole interference rate of the film material and hot pressing treatment.

[0045] h. The inter-hole interference rates obtained in three experiments were 0.015, 0.0023, and 0.000085 respectively.

[0046] The above experimental data show that when the traditional measurement method is used for bioluminescence detection, the well interference coefficient reaches 1.5%, while after the detection is carried out using the technical solution of the utility model, the well interference coefficient can be reduced to less than one ten-thousandth. The technical solution of the utility model can reduce the well interference coefficient by more than two orders of magnitude, which is of great significance for improving the experimental accuracy.

[0047] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the protection scope of the present invention.

Claims

1. A detection device for solving the interference between bioluminescent wells, characterized in that: It comprises a component provided with a plurality of detection units; the bottom of the detection unit is provided with a metal or metal-plated material film.

2. The detection device according to claim 1, characterized in that The metal or metal-plated material film includes one or more of copper foil, aluminum foil, gold foil, tin foil or plastic reflective film.

3. The detection device according to claim 1, characterized in that: The back side of the metal or metal-plated material film comprises an adhesive material, and the metal or metal-plated material film is tightly combined with the bottom of the detection unit through the adhesive material.

4. The detection device according to claim 1, characterized in that: The bottom of the detection unit is provided with a metal or metal-plated material film, which is tightly combined with the bottom of the detection unit by laminating, hot pressing or mechanical processing.

5. The detection device according to claim 4, characterized in that: The hot pressing includes heating the mold so that the metal or metal-plated material film is embedded in the bottom of the detection unit.

6. The detection device according to claim 5, characterized in that: The temperature of the hot pressing is 110° C. and the pressure is 50N.

7. The detection device according to claim 4, characterized in that: The mechanical processing includes processing the bottom of the detection unit into a groove, and using a mold to embed the metal or metal-plated material film into the bottom of the detection unit.

8. The detection device according to claim 1, characterized in that: The side walls of the detection unit are also provided with a metal or metal-plated material film.

9. The detection device according to any one of claims 1 to 8, characterized in that: The component comprises a 6-well plate, a 12-well plate, a 24-well plate, a 48-well plate, a 96-well plate, a 384-well plate or a 1536-well plate, and the bottom of the component is transparent.

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