Gel electrophoresis all-in-one machine

Abstract

The utility model discloses a gel electrophoresis all-in-one machine which adopts the following technical scheme that the gel electrophoresis all-in-one machine comprises a shell, a cavity is arranged in the shell, and a heat dissipation module is arranged in the cavity and used for dissipating heat generated in the cavity during an electrophoresis experiment. A plurality of electrophoresis operation modules are arranged in the cavity, each electrophoresis operation module comprises a body, the body is provided with an illumination area, an excitation light source, an excitation light filter, a light homogenizing film and an emission light filter, and an electrophoresis detection target is placed in the illumination area. According to the gel electrophoresis all-in-one machine disclosed by the utility model, gel electrophoresis is integrated on one instrument from operation to checking, so that the operation is simple, the repeatability is high, the electrophoresis time is short, the cross contamination of samples is reduced, and the electrophoresis operation and the collection of gel maps can be monitored in real time.

Description

Technical Field

[0001] The utility model belongs to the technical field of medical equipment, and particularly relates to a gel electrophoresis integrated machine. Background Art

[0002] After DNA molecules are extracted or amplified, they need to be detected by electrophoresis technology. Existing gel electrophoresis equipment is divided into three large modules, namely three machines: power supply, electrophoresis tank and supporting gel mold, gel observation and photography system. Therefore, the existing electrophoresis method requires multiple time-consuming steps and multiple large equipment for electrophoresis, gel staining, imaging, and image processing and analysis. Therefore, the traditional electrophoresis method has the disadvantages of complex operation, long electrophoresis time, poor result repeatability, and easy cross-contamination of samples. In addition, due to thermal stability and heat dissipation problems of the electrophoresis tank, the Joule heat generated by the gel will also affect the stability of the results, and may even melt the gel and cause the electrophoresis to fail. Utility Model Content

[0003] The utility model provides an integrated gel electrophoresis machine, which performs gel electrophoresis experiments on one instrument, has simple operation, good thermal stability, short electrophoresis time and little sample cross contamination.

[0004] An embodiment of the utility model provides a gel electrophoresis integrated machine, comprising a shell, a cavity is arranged in the shell, and a heat dissipation module is arranged in the cavity for dissipating the heat generated during the electrophoresis experiment.

[0005] Preferably, the heat dissipation module comprises a fan and an air guide groove, and the air guide groove guides the air blown out by the fan to the electrophoresis detection target.

[0006] Preferably, a plurality of electrophoresis operation modules are provided in the cavity, and the electrophoresis operation module comprises a main body, on which an illumination area, an excitation light source, an excitation filter, a uniform light film and an emission filter are provided, and the electrophoresis detection target is placed in the illumination area.

[0007] Preferably, the fan and the air guide groove are located on the body.

[0008] Preferably, the electrophoresis operation module further includes a reflective film.

[0009] Preferably, the housing is provided with a flip cover, the flip cover is provided with an observation window, and the observation window is provided with an emission filter.

[0010] Preferably, the electrophoresis operation module is connected to a main control board, and the main control board is connected to a touch screen.

[0011] Preferably, the air guide slot includes an air outlet and an air inlet, and the fan is located near the air inlet.

[0012] Preferably, the fan and the air guide groove are located on the shell, the air guide groove includes an air inlet and an air outlet, the fan and the air inlet are located on one side of the cavity, and the air outlet is located on the other side of the cavity.

[0013] The gel electrophoresis integrated machine of the utility model has the following beneficial effects:

[0014] 1. The electrophoresis operation model dissipates heat through the heat dissipation module, which reduces the experimental time and improves the stability and repeatability of the results.

[0015] 2. A cavity is provided in the shell, and multiple electrophoresis operation modules are provided in the cavity. If there is a problem with the electrophoresis operation module, the module with the problem can be replaced separately, and the instrument is easy to expand and maintain.

[0016] 3. Set the excitation light source to stimulate the electrophoresis detection target molecule to emit light. You can monitor and check whether the experimental results are completed at any time. You can freely and flexibly control the end time. You can stop the experiment at any time after monitoring and observing the desired results.

[0017] 4. The operation is automated to reduce manual errors, thereby reducing pollution and improving detection accuracy. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is a three-dimensional schematic diagram of the gel electrophoresis integrated machine of the utility model. Figure 1 .

[0019] Figure 2 This is a three-dimensional schematic diagram of the gel electrophoresis integrated machine of the utility model. Figure 2 .

[0020] Figure 3 This is a three-dimensional schematic diagram of the gel electrophoresis integrated machine of the utility model. Figure 3 .

[0021] Figure 4 This is a three-dimensional schematic diagram of the electrophoresis operation module of the utility model. Figure 1 .

[0022] Figure 5 This is a three-dimensional schematic diagram of the electrophoresis operation module of the utility model. Figure 2 .

[0023] Figure 6 This is a three-dimensional schematic diagram of the air guide slot of the utility model. Figure 1 .

[0024] Figure 7 This is a three-dimensional schematic diagram of the air guide slot of the utility model. Figure 2 .

[0025] Figure 8 This is a three-dimensional schematic diagram of the air guide slot of the utility model. Figure 3 .

[0026] Fig. 9 This is a three-dimensional schematic diagram of the air guide slot of the utility model. Figure 4 .

[0027] Fig.10 This is a three-dimensional schematic diagram of the air guide slot of the utility model. Figure 5 .

[0028] Fig.11 This is a three-dimensional schematic diagram of the air guide slot of the utility model. Figure 6 . DETAILED DESCRIPTION

[0029] In order to make the purpose, technical solution and advantages of the utility model clearer, the technical solution of the utility model will be described in detail below. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other implementation methods obtained by ordinary technicians in this field without creative work belong to the scope of protection of the utility model.

[0030] refer to Figure 1-Figure 5 In one embodiment of the utility model, the gel electrophoresis integrated machine includes a housing 1, a cavity is provided in the housing 1, an electrophoresis operation module is provided in the cavity, the electrophoresis operation module is provided with a holder 7 for placing an experimental object 6 for experiment, and the electrophoresis operation module includes an excitation light source 14, an illumination area 15, a reflective film 19, a uniform light film 20 and a filter 21. The excitation light source 14 emits light to the consumables, and the consumables emit light under the irradiation, which is convenient for the experimenter to observe and take pictures. The reflective film 19 is used to irradiate all the light sources of the excitation light source 14 in the same direction, and the uniform light film 20 makes the excitation light evenly irradiate the consumables. The filter 21 includes an excitation light filter and an emission light filter. The excitation light filter filters out stray light and filters out the excitation light of the excitation light wavelength. The emission light filter filters out stray light and filters out the emission light of the emission light wavelength to form an image. In this embodiment, the emission light filter is the observation window 3 on the machine cover, so that the excitation light is evenly irradiated on the consumables.

[0031] The cavity is also provided with waterproof glass 22 and electrode 8. The waterproof glass 22 is used to prevent external liquid from flowing into the cavity. The electrode 8 is connected to the electrophoresis chip to detect whether the electrophoresis chip is placed in the card holder 7. The electrode 8 is also a high-voltage power contact. It can provide high voltage for running electrophoresis when used in conjunction with the main control board 16. The touch screen 2 is provided with a human-computer interaction interface, and the instrument can be controlled through the operation interface. The flip cover 4 is used to cover the electrophoresis reaction area. The electrophoresis experiment process can be seen through the observation window 3. The foot pad 10 is used to place the instrument steadily and form an air intake channel to provide an air flow source for heat dissipation. The power socket 12 is the power input port of the instrument. The power switch 13 is the power button of the instrument. The air outlet 9 is used to exhaust the hot air in the reaction area of ​​the instrument. The air outlet 9 is the air outlet of the instrument, and the air inlet 11 is the air inlet of the instrument.

[0032] refer to Figure 1 , Figure 2 , Figure 4 In one embodiment of the utility model, the gel electrophoresis integrated machine includes a housing 1, and a plurality of electrophoresis operation modules are arranged in a cavity 7. If a problem occurs in an electrophoresis operation module, the module with the problem can be replaced separately, and the instrument is easy to expand and maintain. Each electrophoresis operation module is connected to a main control board 16, and the main control board 16 is connected to a touch screen 2.

[0033] refer to Figure 1-Figure 3 , Figure 6-Figure 11 In one embodiment of the utility model, the gel electrophoresis integrated machine includes a housing 1, a cavity is provided in the housing 1, and a heat dissipation module is provided in the cavity, including a fan 18 and an air guide slot 17. In this embodiment, the heat dissipation system can be composed of two parts, which cooperate with the heat dissipation of the three electrophoresis operation modules. The air guide slot outlet 17-2 on the left is used to blow away the heat in the electrophoresis consumables 6 and the electrophoresis operation module on the left, and the air guide slot outlet 17-2 on the right is used to blow away the heat in the electrophoresis consumables 6 and the electrophoresis operation module on the right. The heat dissipation in the middle electrophoresis consumables 6 and the electrophoresis operation module is completed by the joint action of two air guide slot outlets 17-1, one air guide slot outlet 17-1 is distributed on the top, and the other air guide slot outlet 17-1 is distributed on the bottom. The heat dissipation in the middle electrophoresis consumables 6 and the electrophoresis operation module can use one of the air outlets 17-1 as an air outlet and the other as an air inlet. It can also be blown from the electrophoresis consumables 6 to the side outlet. The heat dissipation air guide 5 is the air outlet of the air guide slot 17, which realizes the air cooling and heat dissipation of the electrophoresis operation module. The air outlet 9 is the outlet of the instrument, and the air inlet 11 is the air inlet of the instrument. The air inlet 17-3 of the air guide slot is divided into two directions of left and right.

[0034] In this embodiment, the heat dissipation system can be implemented in other ways, such as blowing from one side of the consumables platform to the other side to form an air path. The other side can also be sucked by a fan, which makes it easier to form an air path. A large fan can be replaced with a small fan to form an air cooling air path.

[0035] The above is only a specific implementation of the utility model, but the protection scope of the utility model is not limited thereto. Any technician familiar with the technical field can easily think of changes or substitutions within the technical scope disclosed by the utility model, which should be included in the protection scope of the utility model. Therefore, the protection scope of the utility model should be based on the protection scope of the claims.

Claims

1. A gel electrophoresis integrated machine, comprising a housing, characterized in that: A cavity is provided in the shell, and a heat dissipation module is provided in the cavity for dissipating the heat generated in the cavity during the electrophoresis experiment. The heat dissipation module includes a fan and an air guide groove, and the air guide groove guides the wind blown by the fan to the electrophoresis detection target.

2. The gel electrophoresis integrated machine according to claim 1, characterized in that: A plurality of electrophoresis operation modules are arranged in the cavity, and the electrophoresis operation modules include a main body, on which an illumination area, an excitation light source, an excitation filter, a uniform light film and an emission filter are arranged, and the electrophoresis detection target is placed in the illumination area.

3. The gel electrophoresis integrated machine according to claim 2, characterized in that: The fan and the air guide groove are located on the body.

4. The gel electrophoresis integrated machine according to claim 2, characterized in that: The electrophoresis operation module also includes a reflective film.

5. The gel electrophoresis integrated machine according to claim 2, characterized in that: The housing is provided with a flip cover, the flip cover is provided with an observation window, and the observation window is provided with an emission filter.

6. The gel electrophoresis integrated machine according to claim 2, characterized in that: The electrophoresis operation module is connected to a main control board, and the main control board is connected to a touch screen.

7. The gel electrophoresis integrated machine according to claim 3, characterized in that: The air guide slot includes an air outlet and an air inlet, and the fan is located near the air inlet.

8. The gel electrophoresis integrated machine according to claim 2, characterized in that: The fan and the air guide groove are located in the shell, the air guide groove includes an air inlet and an air outlet, the fan and the air inlet are located on one side of the cavity, and the air outlet is located on the other side of the cavity.

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