Noninvasive detection device for nano-particles in blood vessel

Abstract

The utility model discloses a noninvasive detection device for nano-particles in blood vessels, which belongs to the field of blood detection and comprises a carrying table, a luminous light source, an image acquisition component, a laser shaping component and a fluorescence acquisition component, a placing surface is formed on the carrying table, the light-emitting light source is right opposite to the carrying table, the image acquisition assembly is arranged on one side, far away from the placing surface, of the carrying table, the laser shaping assembly is also arranged on one side, far away from the placing surface, of the carrying table, and the fluorescence acquisition assembly and the laser shaping assembly are arranged on the same side. According to the noninvasive detection device for the nano-particles in the blood vessel, the laser shaping assembly is used for exciting fluorescent photons in the blood vessel, and the fluorescent collection assembly is used for collecting the excited fluorescent photons, so that the laser shaping assembly can fully activate the fluorescent photons penetrating through the blood vessel; correspondingly, the fluorescence collection assembly can collect fluorescence photons, so that noninvasive detection on the nano-particles in the blood vessel is realized.

Description

Technical Field

[0001] The utility model belongs to the field of blood detection, and in particular relates to a non-invasive detection device for nanoparticles in blood vessels. Background Art

[0002] Blood circulation is one of the important ways for nanoparticles to be delivered to target organs. Nanoparticles can act on living organisms through intravenous injection, inhalation, subcutaneous absorption, etc., and usually enter the blood circulation first when reaching the target tissue. Detecting the clearance and aggregation of nanoparticles in the blood circulation is of great significance for studying the clearance rate and biocompatibility of nanoparticles in living bodies.

[0003] A typical in vitro detection method based on blood sampling is high performance liquid chromatography technology. High performance liquid chromatography (HPLC) is one of the technical means currently widely used to separate, analyze and purify compounds. The high performance liquid chromatography system is usually composed of an injector, a liquid flow pump, a chromatographic column, a detector, and a data recording and processing device. The HPLC-based nanoparticle quantification method is achieved by measuring the fluorescence intensity. Nanoparticles are fluorescent after being labeled with fluorescein, and the fluorescein loading of a single nanoparticle is relatively certain. Therefore, the concentration information of fluorescein can be obtained through the fluorescence intensity of fluorescein, and then the concentration information of nanoparticles can be obtained through the fluorescence intensity of fluorescein, and then the concentration information of nanoparticles can be obtained through the fluorescence intensity-nanoparticle concentration calibration curve.

[0004] Another typical in vitro detection technology based on blood sampling is dynamic light scattering (DLS). The dynamic light scattering method measures the particle size by measuring the change of light fluctuation intensity over time. When the particles to be tested are suspended in a liquid, irregular Brownian motion will occur. The speed of the Brownian motion of the particles will be affected by the particle size and the viscosity of the liquid. The smaller the particle size and the smaller the viscosity of the liquid in which the particles are suspended, the faster the particles will perform irregular Brownian motion. This irregular Brownian motion will affect the intensity of the light detected by the DLS instrument. When the detection light of the DLS instrument passes through the colloid composed of particles and liquid, light scattering will occur due to the particles. The DLS light detector can detect the scattered light signals generated by these particles at a certain angle. Therefore, the particle size can be quantitatively characterized by the amplitude and correlation of the light intensity fluctuation.

[0005] The in vitro detection method based on blood sampling separates the nanoparticles from their original biological living environment. The test results are often quite different from the actual state of the nanoparticles in the living organism. In addition, the overall structure is complex, the operation is cumbersome, and the detection efficiency is low, which cannot meet the needs of today's fast and accurate detection operations. Utility Model Content

[0006] In response to one or more of the above defects or improvement needs of the prior art, the utility model provides a non-invasive detection device for intravascular nanoparticles, which utilizes a laser shaping component to excite fluorescent photons in the blood vessel, and collects the excited fluorescent photons through a fluorescence collection component, and at the same time adjusts the position of the carrier through an image collection component so that the linear light beam emitted by the laser shaping component can completely cross the cross-section of the blood vessel, thereby enabling the laser shaping component to fully activate the fluorescent photons passing through the blood vessel, and correspondingly enabling the fluorescence collection component to fully collect the fluorescent photons, so as to achieve non-invasive detection of intravascular nanoparticles.

[0007] To achieve the above-mentioned purpose, the utility model provides a non-invasive detection device for intravascular nanoparticles, which is used to detect animal blood vessels injected with nano fluorescent particles, and is characterized in that:

[0008] It includes a stage, a light source, an image acquisition component and a laser shaping component, as well as a fluorescence acquisition component;

[0009] The platform is formed with a placement surface for carrying an animal injected with fluorescent nanoparticles, and the light source is arranged facing the platform for irradiating the blood vessels of the animal on the platform;

[0010] The image acquisition component is arranged on a side of the platform away from the placement surface, and is used to acquire image data of animal blood vessels;

[0011] The laser shaping component is also arranged on a side of the carrier away from the placement surface, and is used to emit a laser beam into the animal's blood vessel and form a linear light spot to excite fluorescent photons in the animal's blood vessel;

[0012] The fluorescence collection component and the laser shaping component are arranged on the same side, and the axes of the two are parallel, and are used to collect fluorescent photons in the blood vessels of animals and convert them into electrical signals;

[0013] As a further improvement of the utility model, the image acquisition component includes an imaging objective lens, a laser processing component, and a CCD camera;

[0014] The imaging objective lens is arranged on a side of the carrier away from the placement surface, and is used to collect the illumination light that penetrates the animal's blood vessels. The laser processing component is arranged between the lens of the CCD camera and the imaging objective lens, and is used to process the illumination light collected by the imaging objective lens and focus it in the CCD camera to form an image of the mouse ear blood vessels.

[0015] As a further improvement of the present utility model, the laser processing assembly includes a first filter and a first achromatic lens;

[0016] The first filter and the first achromatic lens are arranged on the axis of the CCD camera, and are used for filtering the detection laser and converging the illumination light respectively.

[0017] As a further improvement of the utility model, the laser shaping assembly includes a laser light source, a laser shaping unit and a beam splitter arranged at the emission end of the laser light source, and the microscope objective lens;

[0018] The microscope objective lens is arranged on a side of the stage away from the placement surface, and the beam splitter is arranged between the microscope objective lens and the laser light source, and is used to reflect the laser light emitted by the laser light source into the microscope objective lens after being processed by the laser shaping unit, and focus the laser beam through the microscope objective lens to form a linear light spot, so that the linear light spot covers the animal blood vessel.

[0019] As a further improvement of the utility model, the laser shaping unit includes a cylindrical lens, a mechanical slit and a second achromatic lens;

[0020] The cylindrical lens, the mechanical slit and the second achromatic lens are arranged at intervals along the axial direction of the laser light source; the cylindrical lens is used to compress the laser light emitted by the laser light source and form a long ellipse; and the light beam is spatially filtered and shaped through the mechanical slit, and finally converged through the second achromatic lens and guided to the microscope objective through a spectroscope.

[0021] As a further improvement of the present invention, the laser light source adopts a semiconductor laser with a central wavelength of 488nm.

[0022] As a further improvement of the utility model, the fluorescence collection component includes a reflector, a laser processing component and a photomultiplier tube;

[0023] The reflector is located on the extension line of the line connecting the centers of the beam splitter and the microscope objective lens, and is used to receive fluorescent photons emitted from the animal blood vessels after the laser shaping component irradiates the fluorescent substance in the animal blood vessels; the fluorescence processing unit is arranged between the photomultiplier tube and the reflector, and is used to process the fluorescent photons received by the reflector and transmit them to the photomultiplier tube.

[0024] As a further improvement of the present utility model, the fluorescence processing component includes a second filter and a third achromatic lens;

[0025] The second filter and the third achromatic lens are arranged at intervals on the axis of the photomultiplier tube, and are used to filter out interference caused by the excitation light from the laser shaping unit and converge the fluorescent light beam to the mechanical slit at the front end of the photomultiplier tube.

[0026] As a further improvement of the utility model, the light source adopts an LED light source with a wavelength of 535nm;

[0027] An XY linear motor is also provided corresponding to the carrier, which is used to adjust the position of the carrier so that the linear beam formed by the laser shaping unit can cross the blood vessels of the animal of interest. The above-mentioned improved technical features can be combined with each other as long as they do not conflict with each other.

[0028] In general, compared with the prior art, the above technical solutions conceived by the utility model have the following beneficial effects:

[0029] (1) The utility model is a non-invasive detection device for intravascular nanoparticles. It uses a laser shaping component to excite fluorescent photons in the blood vessel, and uses a fluorescence collection component to collect the excited fluorescent photons. At the same time, the position of the carrier is adjusted by the image collection component so that the linear light beam emitted by the laser shaping component can completely cross the cross-section of the blood vessel, thereby enabling the laser shaping component to fully activate the fluorescent substance passing through the blood vessel, and correspondingly enabling the fluorescence collection component to fully collect the fluorescent photons, so as to achieve non-invasive detection of intravascular nanoparticles.

[0030] (2) The non-invasive detection device for intravascular nanoparticles of the utility model adopts a split design for the image acquisition component and the laser shaping component, so that the image acquisition component can switch the image magnification. The freedom of the imaging magnification of the microvascular image navigation part is improved, which facilitates imaging and finding blood vessels at different detection sites.

[0031] (3) The non-invasive detection device for intravascular nanoparticles of the utility model optimizes the design of the fluorescence receiving unit and the laser shaping component so that the two share the same optical path, thereby reducing the loss of each part of light and improving the utilization rate of the excitation light source and the fluorescence collection efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 It is a schematic diagram of the overall structure of the non-invasive detection device in the embodiment of the utility model;

[0033] Figure 2 It is a diagram defining characteristic components of a fluorescence data signal in a non-invasive detection device in an embodiment of the utility model;

[0034] Figure 3 It is a typical fluorescence data curve diagram obtained in the non-invasive detection device in the embodiment of the utility model;

[0035] In all the drawings, the same reference numerals represent the same technical features, specifically:

[0036] 1. Light source; 2. Stage; 3. Imaging objective lens; 4. First filter; 5. First achromatic lens; 6. CCD camera; 7. Display screen; 8. Microscope objective lens; 9. Spectroscope; 10. Second achromatic lens; 11. Mechanical slit; 12. Cylindrical lens; 13. Laser light source; 14. Reflector; 15. Second filter; 16. Third achromatic lens; 17. Photomultiplier tube. DETAILED DESCRIPTION

[0037] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments.

[0038] It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention. In addition, the technical features involved in each embodiment of the present invention described below can be combined with each other as long as there is no conflict between them.

[0039] See also Figures 1 to 3 The non-invasive detection device for intravascular nanoparticles in the preferred embodiment of the utility model is used to detect the blood vessels of animals injected with nano fluorescent particles.

[0040] It is understandable that the above-mentioned nanoparticles can be materials with fluorescence characteristics, or can be a carrier adsorbing or encapsulating fluorescein. The fluorescent particles in the preferred embodiment of the utility model are polylactic acid nanoparticles PLA-PEG modified with polyethylene glycol. The nanoparticles are labeled with coumarin-6, and the maximum absorption wavelength is 459.2nm. Therefore, the PLA-PEG nanoparticles can be excited by the 488nm laser of the device. The fluorescence of 505nm to 525nm wavelength emitted by the nanoparticles can be collected and received by the fluorescence collection component of the device.

[0041] In the actual application process, nanoparticles can enter the blood circulation through various ways, such as injection, oral administration, inhalation, subcutaneous absorption, etc. In the present invention, it is preferred to directly enter the blood circulation by tail vein injection.

[0042] In a specific application scenario, the object to be tested is a mouse, and the part to be tested is the ear of the mouse. The technical solution of the utility model is further described below in combination with the above application scenario.

[0043] Specifically, the non-invasive detection device in the preferred embodiment of the utility model includes a carrier 2, a light source 1, an image acquisition component and a laser shaping component, and a fluorescence acquisition component; a placement surface is formed on the carrier 2 for carrying an animal injected with fluorescent nanoparticles; and the light source 1 is arranged opposite to the carrier 2 for irradiating the animal blood vessels on the carrier 2; the image acquisition component is arranged on the side of the carrier 2 away from the placement surface, for collecting image data of the animal blood vessels; the laser shaping component is also arranged on the side of the carrier 2 away from the placement surface, for emitting a laser beam into the animal blood vessels, and forming a linear spot to excite fluorescent photons in the animal blood vessels; the fluorescence acquisition component and the laser shaping component are arranged on the same side, and the axes of the two are parallel, which are used to collect fluorescent photons in the animal blood vessels and convert them into electrical signals.

[0044] More specifically, the image acquisition component includes an imaging objective lens 3 and a laser processing component CCD camera 6; the imaging objective lens 3 is arranged on the side of the stage 2 away from the placement surface, and is used to collect the illumination light that penetrates the animal's blood vessels. The laser processing component is arranged between the lens of the CCD camera 6 and the imaging objective lens 3, and is used to process the illumination light collected by the imaging objective lens 3 and focus it in the CCD camera 6 to form an image of the mouse ear blood vessels.

[0045] Preferably, the light source 1 uses an LED light source with a wavelength of 535nm. In actual use, the green illumination light emitted by the LED light source will first reach the carrier 2 and illuminate the ear of the mouse. Since the tissue structure of the mouse ear is relatively thin, part of the illumination light will be absorbed by the mouse ear, thereby attenuating the brightness of the illumination light. The remaining illumination light will penetrate the mouse ear and then be collected by the imaging objective lens 3, and the collected illumination light will enter the image guide unit.

[0046] More preferably, if Figure 1 As shown in FIG. 1 , the laser processing assembly includes a first filter 4 and a first achromatic lens 5 ; the first filter 4 and the first achromatic lens 5 are arranged on the axis of a CCD camera 6 , and are used to filter the detection laser and converge the illumination light respectively.

[0047] In the actual detection process, detection lasers of different wavelengths will also enter the image guide unit together under the action of the microscope objective lens 8. Therefore, this part of the light will first be filtered by the first filter 4 to remove the interference of the detection laser on the 535nm imaging illumination light. The filtered illumination light is focused on the CCD camera 6 behind by the convergence of the first achromatic lens 5. Finally, the green transmitted light entering the CCD camera 6 carries the structural information of the mouse ear, and finally forms an image of the mouse ear blood vessels, and the image is mapped on the display screen 7.

[0048] In more detail, the laser shaping assembly includes a laser light source 13, a laser shaping unit and a beam splitter 9 arranged at the emission end of the laser light source 13, and a microscope objective lens 8; the microscope objective lens 8 is arranged on the side of the stage 2 away from the placement surface, and the beam splitter 9 is arranged between the microscope objective lens 8 and the laser light source 13, and is used to reflect the laser emitted by the laser light source 13 into the microscope objective lens 8 after being processed by the laser shaping unit, and focus the laser beam through the microscope objective lens 8 to form a linear light spot, and make the linear light spot cover the animal blood vessel of interest.

[0049] Furthermore, the laser shaping unit includes a cylindrical lens 12, a mechanical slit 11 and a second achromatic lens 10; the cylindrical lens 12, the mechanical slit 11 and the second achromatic lens 10 are arranged at intervals along the axial direction of the laser light source 13; the cylindrical lens 12 is used to compress the laser light emitted by the laser light source 13 and form a long ellipse; and the light beam is spatially filtered and shaped through the mechanical slit 11, and finally converged through the second achromatic lens 10, and refracted to the microscope objective 8 through the beam splitter 9.

[0050] In the actual application process, the laser shaping component modulates the shape of the laser beam emitted by the laser light source 13 at the focal plane of the objective lens into a long linear light spot. Preferably, the laser light source 13 adopts a semiconductor laser with a central wavelength of 488nm. The laser beam undergoes compression in one dimension under the action of the cylindrical lens 12. At this time, the cross-section of the laser beam is compressed from the original circular shape to a long elliptical shape. Then a mechanical slit 11 is inserted into the incident light path for spatial filtering and shaping of the beam. Finally, the laser beam is converged by the second achromatic lens 10 and then guided to the microscope objective 8 through the beam splitter 9. The microscope objective 8 further focuses the laser beam to form a long linear light spot. At this time, the operator can observe the display screen 7 of the image navigation component and adjust the horizontal position of the stage 2, so that the linear laser light spot will eventually cross the selected arteriolar blood vessels.

[0051] Furthermore, if Figure 1 As shown in the figure, the fluorescence collection component includes a reflector 14, a laser processing component and a photomultiplier tube 17; the reflector 14 is located on the extension line of the center line of the beam splitter 9 and the microscope objective 8, and is used to receive the fluorescent photons emitted from the animal blood vessels after the laser shaping component irradiates the fluorescent substance in the animal blood vessels; the fluorescence processing unit is arranged between the photomultiplier tube 17 and the reflector 14, and is used to process the fluorescent photons received by the reflector 14 and transmit them to the photomultiplier tube 17.

[0052] In more detail, the fluorescence processing component includes a second filter 15 and a third achromatic lens 16; the second filter 15 and the third achromatic lens 16 are arranged at intervals on the axis of the photomultiplier tube 17, which are used to filter out interference caused by the excitation light from the laser shaping unit and converge the fluorescence light beam to the mechanical slit 11 at the front end of the photomultiplier tube 17.

[0053] In actual application, when the fluorescently labeled nanoparticles flow through the laser-irradiated detection area in the blood vessels, they will be excited, thereby releasing fluorescent photons in all directions. A portion of the fluorescent photons directed toward the microscope objective 8 are collected by the first microscope objective and directed to the fluorescence processing unit through the beam splitter 9 and the reflector 14. The second filter 15 in the fluorescence processing unit filters out the interference caused by the excitation light from the laser light source 13, and the fluorescent light beam is converged to the mechanical slit 11 by the third achromatic lens 16. The fluorescence passing through the mechanical slit 11 will be received by the photomultiplier tube 17, and the fluorescent signal will be converted into an electrical signal that can be collected and recorded. In addition, the electrical signal will be amplified by the signal amplifier and stored by the data acquisition card.

[0054] Furthermore, in order to more conveniently analyze the fluorescence signal, we defined the parameters of the fluorescence signal obtained by the device, as shown in the attached figure. Figure 2As shown in the figure, the relatively flat part of the fluorescence signal is defined as the baseline signal, and the size of the baseline level is defined as the difference between the background fluorescence signal intensity before and after the nanoparticle injection. The observed pulse-like fluorescence signal is defined as the peak signal.

[0055] In order to discuss the detailed characteristics of the peak signal in more detail, the peak signal is described by two characteristic parameters: peak height and peak width. The peak height is defined as the difference between the highest point of the peak signal and the baseline level before nanoparticle injection. The peak width is defined as the length of time that the pulse-like peak signal is higher than the baseline level.

[0056] Furthermore, as attached Figure 3 As shown in the figure, with the time point of nanoparticle injection as the coordinate origin 0, it can be observed that the period from -300s to 0s is the detection time before the nanoparticle injection. During this detection time, the signal intensity fluctuation of the fluorescence data is relatively small. This low-intensity fluorescence signal is the background signal during in vivo detection, which is mainly formed by partial autofluorescence of animal tissues and blood and partial reflected excitation light.

[0057] After the nanoparticles were injected into the blood circulation of mice, the level of the fluorescent signal received by the device increased significantly, and this increased signal level gradually decayed over time. At the same time, pulse-like peak signals can also be observed in the fluorescent signal. These peak signals have a certain height and width, and the frequency of these peak signals will also change over time. In the early stage of the injection of nanoparticles into the blood circulation of mice, a large number of pulse-like peak signals with large peak heights and peak widths appeared. Subsequently, the frequency of pulse-like peak signals gradually decreased, and accordingly, the peak height of the peak signal also became smaller than in the early stage, and the peak width also gradually narrowed.

[0058] In actual application, an XY linear motor is also provided corresponding to the carrier 2 to adjust the position of the carrier 2 so that the linear beam formed by the laser shaping unit can cross the entire animal blood vessel.

[0059] It is worth noting that in the preferred embodiments of the present invention, the various processes for light path processing and the methods for exciting fluorescent molecules are functions that can be understood by those skilled in the art based on the device names, and do not involve data processing processes.

[0060] (1) The utility model is a non-invasive detection device for intravascular nanoparticles. It uses a laser shaping component to excite fluorescent photons in the blood vessel, and uses a fluorescence collection component to collect the excited fluorescent photons. At the same time, the position of the carrier is adjusted by the image collection component so that the linear light beam emitted by the laser shaping component can completely cross the cross-section of the blood vessel of interest, thereby enabling the laser shaping component to fully activate the fluorescent photons passing through the blood vessel, and correspondingly enabling the fluorescence collection component to fully collect the fluorescent photons, so as to achieve non-invasive detection of intravascular nanoparticles.

[0061] (2) The non-invasive detection device for intravascular nanoparticles of the utility model adopts a split design for the image acquisition component and the laser shaping component, so that the image acquisition component can switch the image magnification. The freedom of the imaging magnification of the microvascular image navigation part is improved, which facilitates imaging and finding blood vessels at different detection sites.

[0062] (3) The non-invasive detection device for intravascular nanoparticles of the utility model optimizes the design of the fluorescence receiving unit and the laser shaping component so that the two share the same optical path, thereby reducing the loss of each part of light and improving the utilization rate of the excitation light source and the fluorescence collection efficiency.

[0063] It is easy for those skilled in the art to understand that the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A non-invasive detection device for intravascular nanoparticles, used for detecting blood vessels of animals injected with nano fluorescent particles, characterized in that: It comprises a carrier (2), a light source (1), an image acquisition component and a laser shaping component, and a fluorescence acquisition component; The carrier (2) is provided with a placement surface for carrying an animal injected with fluorescent nanoparticles, and the light source (1) is arranged facing the carrier (2) for irradiating the blood vessels of the animal on the carrier (2); The image acquisition component is arranged on a side of the carrier (2) away from the placement surface, and is used to collect image data of the animal's blood vessels; the image acquisition component comprises an imaging objective lens (3), a laser processing component and a CCD camera (6); the imaging objective lens (3) is arranged on a side of the carrier (2) away from the placement surface, and is used to collect illumination light that penetrates the animal's blood vessels; the laser processing component is arranged between the lens of the CCD camera (6) and the imaging objective lens (3), and is used to process the illumination light collected by the imaging objective lens (3), and focus the light in the CCD camera (6) to form an image of the mouse's ear blood vessels; The laser shaping component is also arranged on a side of the carrier (2) away from the placement surface, and is used to emit a laser beam into the animal's blood vessel, and form a linear light spot to excite fluorescent photons in the animal's blood vessel; the laser shaping component comprises a laser light source (13), a laser shaping unit and a beam splitter (9) arranged at the emission end of the laser light source (13), and a microscope objective lens (8); the microscope objective lens (8) is arranged on a side of the carrier (2) away from the placement surface, and the beam splitter (9) is arranged between the microscope objective lens (8) and the laser light source (13), and is used to reflect the laser light emitted by the laser light source (13) into the microscope objective lens (8) after being processed by the laser shaping unit, and to focus the laser beam through the microscope objective lens (8) to form a linear light spot, so that the linear light spot covers the animal's blood vessel; The fluorescence collection component and the laser shaping component are arranged on the same side, and the axes of the two are parallel, and are used to collect fluorescent photons in the blood vessels of animals and convert them into electrical signals; the fluorescence collection component includes a reflector (14), a laser processing component and a photomultiplier tube (17); The reflector (14) is located on the extension line of the center line of the beam splitter (9) and the microscope objective lens (8), and is used to receive fluorescent photons emitted from the animal blood vessels after the laser shaping component irradiates the fluorescent substance in the animal blood vessels; the fluorescence processing unit is arranged between the photomultiplier tube (17) and the reflector (14), and is used to process the fluorescent photons received by the reflector (14) and transmit them to the photomultiplier tube (17).

2. The non-invasive detection device for intravascular nanoparticles according to claim 1, characterized in that: The laser processing assembly comprises a first filter (4) and a first achromatic lens (5); The first filter (4) and the first achromatic lens (5) are arranged on the axis of the CCD camera (6), and are used to filter the detection laser and to converge the illumination light respectively.

3. The non-invasive detection device for intravascular nanoparticles according to claim 1, characterized in that: The laser shaping unit comprises a cylindrical lens (12), a mechanical slit (11) and a second achromatic lens (10); The cylindrical lens (12), the mechanical slit (11) and the second achromatic lens (10) are arranged at intervals along the axial direction of the laser light source (13); the cylindrical lens (12) is used to compress the laser light emitted by the laser light source (13) and form a long elliptical shape; and to perform spatial filtering and shaping on the light beam through the mechanical slit (11), and finally converge through the second achromatic lens (10), and guide to the microscope objective lens (8) through the beam splitter (9).

4. The non-invasive detection device for intravascular nanoparticles according to claim 3, characterized in that: The laser light source (13) adopts a semiconductor laser with a central wavelength of 488nm.

5. The non-invasive detection device for intravascular nanoparticles according to claim 1, characterized in that: The fluorescence processing component comprises a second filter (15) and a third achromatic lens (16); The second filter (15) and the third achromatic lens (16) are arranged at intervals on the axis of the photomultiplier tube (17), and are used to filter out interference caused by the excitation light from the laser shaping unit and converge the fluorescent light beam to the mechanical slit (11) at the front end of the photomultiplier tube (17).

6. The non-invasive detection device for intravascular nanoparticles according to claim 1, characterized in that: The light source (1) is an LED light source with a wavelength of 535 nm; An XY linear motor is also provided corresponding to the carrier (2) for adjusting the position of the carrier (2) so that the linear light beam formed by the laser shaping component can cross the blood vessel of the animal of interest.

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