Simple NKT cell migration device

Abstract

The utility model discloses a simple NKT cell migration device, which relates to the technical field of cell culture devices and comprises a sample storage box, a culture tank erected at the top in the sample storage box and a rotating shaft rotationally arranged in the culture tank, and one end of the rotating shaft protrudes out of one side of the culture tank. According to different oxygen content requirements during actual culture, the worker connects a one-way air inlet pipe with external equipment, conveys gas with different oxygen concentrations, and drives a connecting rod and a pressing seat to periodically move up and down in a reciprocating manner through a rotating shaft and a vertical rod, so that an air bag is compressed and stretched, and when the air bag is compressed, the air bag is compressed; according to the cell culture device, gas in the cell culture device enters the annular pipe through the one-way exhaust pipe and then is uniformly diffused into a culture solution through the micropores formed in the annular pipe, so that the oxygen content in the culture solution is increased or reduced, the oxygen content requirements during culture of different cells are met, the cells are not easy to die, and a great guarantee is provided for subsequent experiments.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture devices, in particular to a simple device for NKT cell migration. Background Art

[0002] NKT cells are a special T cell subset that have both T cell receptors TCR and NK cell receptors on their cell surface. NKT cells can produce a large number of cytokines and can exert cytotoxic effects similar to NK cells. In some cell experiments, in order to facilitate the observation of cell migration dynamics, experimenters usually need to transfer cells to cell dishes before cell migration.

[0003] The Chinese patent authorization announcement number is CN209797988U, and its name is a simple device for NKT cell migration. A vibration box is arranged inside the migration box, and shaking springs are fixed on both sides of the vibration box. The other ends of the shaking springs are respectively fixed on the inner walls of both sides of the migration box. The upper and lower ends of the vibration box are connected with hoses. A tamping rod is inserted on one side wall of the migration box and below one of the shaking springs. The bottom end of the lower hose is connected with a storage box, and the bottom of the storage box is connected with a conduit. A hand-held squeezing ball is connected on the conduit and located on the outside of the migration box. A shaking device is arranged inside the migration box to drive the vibration box to shake, so that the cells can maintain good activity in the culture medium, which is convenient for improving cell vitality. A guide plug, a reset spring and a sealing cover plate combination are arranged inside the conduit, and they are used together to control the unidirectional flow of cells and prevent the air in the hand-held squeezing ball from entering the storage box, thereby improving the accuracy of the detection data.

[0004] The shortcomings of the above-mentioned prior art solutions are: although the above-mentioned solution can shake the vibration box to maintain cell activity, during the cell culture process, different cells have different requirements for the oxygen content in the culture solution. When the device cultures cells, it is difficult to meet the oxygen content requirements of different cell growth environments, which can easily cause cell death and cause many inconveniences to subsequent experimental work. Utility Model Content

[0005] The purpose of the utility model is to provide a simple NKT cell migration device to solve the technical problems mentioned in the above background technology.

[0006] The technical problem to be solved by the utility model can be achieved through the following technical solutions:

[0007] A simple NKT cell migration device comprises a sample storage box, a culture tank mounted on the top of the sample storage box, and a rotating shaft rotatably arranged inside the culture tank, wherein one end of the rotating shaft protrudes from one side of the culture tank;

[0008] A vertical rod is provided at one end of the rotating shaft protruding from the culture tank, a connecting rod is movably sleeved at one end of the vertical rod, an air bag is provided near the inner wall of one side of the sample storage box, a one-way air inlet pipe is provided at the input end of the air bag, a one-way exhaust pipe is provided at the output end of the air bag, a pressing seat is provided at the top of the air bag, and the pressing seat is movably connected to the bottom end of the connecting rod;

[0009] An annular tube is arranged around the inner bottom of the culture tank, a breathable film is fitted on the inner wall of the annular tube, micropores are opened on the outer wall of the annular tube, and the input end of the annular tube is connected to the output end of the one-way exhaust pipe.

[0010] As a further solution of the utility model: a stirring frame is sleeved on the outer side of the rotating shaft, a stirring plate is arranged at the bottom of the stirring frame, and the stirring plate is located on the inner side of the annular tube.

[0011] As a further solution of the utility model: a connecting pipe is provided at the bottom of the culture tank, a dilution box is provided at the bottom end of the connecting pipe, a sample outlet tube is provided at the bottom end of the dilution box, and the sample outlet tube passes through the bottom of the sample storage box, and a servo motor for driving the rotating shaft to rotate is provided near the inner wall of the other side of the sample storage box.

[0012] As a further solution of the utility model: a liquid box is arranged on one side of the sample storage box, a liquid infusion tube is arranged at the bottom of the liquid box, and the output end of the liquid infusion tube is connected to the dilution box.

[0013] As a further solution of the utility model: a sealing cover is arranged on the top of the sample storage box, and a ventilation hole is arranged on the sealing cover, and a bracket is arranged on the bottom of the sample storage box.

[0014] As a further solution of the utility model: the inner sides of the connecting pipe and the sample outlet pipe are both provided with magnetic control valves for controlling the opening and closing of the flow channel.

[0015] Beneficial effects of the utility model:

[0016] When the utility model is culturing cells, the staff also connects the one-way air inlet pipe with external equipment according to the different oxygen content requirements during actual cultivation, and transports gases with different oxygen concentrations. The connecting rod and the pressing seat are driven by the rotating shaft and the vertical rod to perform periodic up and down reciprocating motions, thereby compressing and stretching the airbag. When the airbag is compressed, the gas inside it enters the annular tube through the one-way exhaust pipe, and then diffuses into the culture medium evenly through the micropores opened on the annular tube, thereby increasing or reducing the oxygen content in the culture medium, meeting the oxygen content requirements of different cell cultures, and making it difficult for cells to die, which provides a great guarantee for subsequent experiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] The utility model is further described below in conjunction with the accompanying drawings.

[0018] Figure 1 It is a three-dimensional schematic diagram of the whole device in the utility model;

[0019] Figure 2 This utility model Figure 1 The enlarged schematic diagram at A in the middle;

[0020] Figure 3 This utility model Figure 1 Enlarged schematic diagram of point B in the middle.

[0021] In the figure: 1. sample storage box; 2. culture tank; 3. rotating shaft; 4. vertical rod; 5. connecting rod; 6. air bag; 7. one-way air inlet pipe; 8. one-way exhaust pipe; 9. pressing seat; 10. annular tube; 11. breathable membrane; 12. micropores; 13. stirring rack; 14. stirring plate; 15. connecting pipe; 16. dilution box; 17. sample outlet tube; 18. servo motor; 19. liquid box; 20. infusion tube; 21. sealing cover; 22. bracket. DETAILED DESCRIPTION

[0022] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0023] like Figure 1-3As shown, a simple NKT cell migration device comprises a sample storage box 1, a culture tank 2 mounted on the top of the sample storage box 1, a rotating shaft 3 rotatably arranged inside the culture tank 2, and one end of the rotating shaft 3 protrudes from one side of the culture tank 2, a stirring frame 13 is sleeved on the outer side of the rotating shaft 3, a stirring plate 14 is arranged at the bottom of the stirring frame 13, and the stirring plate 14 is located on the inner side of the annular tube 10, a connecting pipe 15 is arranged at the bottom of the culture tank 2, a dilution box 16 is arranged at the bottom end of the connecting pipe 15, a sample outlet tube 17 is arranged at the bottom end of the dilution box 16, and the sample outlet tube 17 runs through the bottom of the sample storage box 1, a servo motor 18 for driving the rotating shaft 3 to rotate is arranged near the inner wall of the other side of the sample storage box 1, a liquid tank 19 is arranged on one side of the sample storage box 1, and a liquid tank 19 is arranged at the bottom An infusion tube 20 is provided, and the output end of the infusion tube 20 is connected to the dilution box 16. When the device is in use, the culture tank 2 is used to store cells and culture fluid. The stirring frame 13 and the stirring plate 14 are driven to rotate by the servo motor 18, thereby stirring the cells in the culture tank 2, thereby avoiding the reduction of cell activity and the occurrence of coagulation. When sampling is required, the culture fluid and cells in the culture tank 2 enter the dilution box 16 through the connecting tube 15, and then are discharged through the sample outlet tube 17. When the number of cells in the culture fluid entering the dilution box 16 is large, the culture fluid stored in the liquid box 19 can be input into the dilution box 16 through the infusion tube 20, thereby diluting the cell density in the original culture fluid, which is convenient for subsequent observation experiments;

[0024] A vertical rod 4 is provided at one end of the rotating shaft 3 protruding from the culture tank 2, and a connecting rod 5 is movably sleeved at one end of the vertical rod 4. An air bag 6 is provided near the inner wall of one side of the sample storage box 1, and a one-way air inlet pipe 7 is provided at the input end of the air bag 6, and a one-way exhaust pipe 8 is provided at the output end of the air bag 6. A pressing seat 9 is provided on the top of the air bag 6, and the pressing seat 9 is movably connected to the bottom end of the connecting rod 5. An annular tube 10 is provided around the inner bottom of the culture tank 2, and a breathable membrane 11 is provided on the inner wall of the annular tube 10. Micropores 12 are provided on the outer wall of the annular tube 10, and the input end of the annular tube 10 is connected to the output end of the one-way exhaust pipe 8. When the rotating shaft 3 rotates, the vertical rod 4 rotates with it, so that the vertical rod 4 can drive the connecting rod 5 The connecting rod 5 drives the pressing seat 9 to press and stretch the airbag 6 by performing periodic up and down reciprocating motion. When the airbag 6 is compressed, the air inside it enters the annular tube 10 through the one-way exhaust pipe 8. The air can be diffused into the culture solution through the micropores 12 opened on the annular tube 10, thereby increasing the oxygen content in the culture solution. The micropores 12 can ensure that the air is evenly diffused into the culture solution, ensuring high efficiency during adjustment. The breathable membrane 11 prevents the culture solution from entering the annular tube 10 while ensuring the passage of gas molecules, thereby preventing pollution to subsequent use. The staff also connects the one-way air inlet pipe 7 to external equipment according to the different oxygen content requirements during actual culture, so as to transport gases with different oxygen concentrations.

[0025] In this embodiment, specifically, a sealing cover 21 is disposed on the top of the sample storage box 1 , and a ventilation hole is disposed on the sealing cover 21 , and a bracket 22 is disposed on the bottom of the sample storage box 1 .

[0026] In this embodiment, specifically, magnetic control valves for controlling the opening and closing of the flow channel are disposed inside the connecting tube 15 and the sample outlet tube 17, thereby controlling the flow rate of the culture solution.

[0027] The above is a detailed description of an embodiment of the utility model, but the content is only a preferred embodiment of the utility model and cannot be considered to limit the scope of implementation of the utility model. All equivalent changes and improvements made within the scope of application of the utility model should still fall within the scope of the patent coverage of the utility model.

Claims

1. A simple NKT cell migration device, comprising a sample storage box (1), a culture tank (2) mounted on the top of the sample storage box (1), a rotating shaft (3) rotatably arranged inside the culture tank (2), and one end of the rotating shaft (3) protruding from one side of the culture tank (2); characterized in that: A vertical rod (4) is provided at one end of the rotating shaft (3) protruding from the culture tank (2), a connecting rod (5) is movably sleeved at one end of the vertical rod (4), an air bag (6) is provided near the inner wall of one side of the sample storage box (1), a one-way air inlet pipe (7) is provided at the input end of the air bag (6), a one-way exhaust pipe (8) is provided at the output end of the air bag (6), a pressing seat (9) is provided at the top of the air bag (6), and the pressing seat (9) is movably connected to the bottom end of the connecting rod (5); An annular tube (10) is arranged around the inner bottom of the culture tank (2), a gas permeable membrane (11) is fitted on the inner wall of the annular tube (10), micropores (12) are opened on the outer wall of the annular tube (10), and the input end of the annular tube (10) is connected to the output end of the one-way exhaust pipe (8).

2. A simple device for NKT cell migration according to claim 1, characterized in that: A stirring frame (13) is sleeved on the outer side of the rotating shaft (3), a stirring plate (14) is arranged at the bottom of the stirring frame (13), and the stirring plate (14) is located on the inner side of the annular tube (10).

3. A simple device for NKT cell migration according to claim 1, characterized in that: A connecting pipe (15) is provided at the bottom of the culture tank (2), a dilution box (16) is provided at the bottom of the connecting pipe (15), a sample outlet pipe (17) is provided at the bottom of the dilution box (16), and the sample outlet pipe (17) passes through the bottom of the sample storage box (1), and a servo motor (18) for driving the rotating shaft (3) to rotate is provided near the inner wall of the other side of the sample storage box (1).

4. The simple device for NKT cell migration according to claim 1, characterized in that: A liquid box (19) is provided on one side of the sample storage box (1), a liquid infusion tube (20) is provided at the bottom of the liquid box (19), and the output end of the liquid infusion tube (20) is connected to the dilution box (16).

5. The simple device for NKT cell migration according to claim 1, characterized in that: The top of the sample storage box (1) is provided with a sealing cover (21), and the sealing cover (21) is provided with ventilation holes, and the bottom of the sample storage box (1) is provided with a bracket (22).

6. The simple device for NKT cell migration according to claim 3, characterized in that: The inner sides of the connecting pipe (15) and the sample outlet pipe (17) are both provided with magnetic control valves for controlling the opening and closing of the flow channel.

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