Reagent dropwise adding device for fluorescence detection

Abstract

The reagent dropping device comprises a supporting plate, a mounting frame, a piston block, a shell, a connecting pipe, a driving assembly, a transmission assembly, a piston cylinder and a piston block, when the reagent dropping device is used, a sample to be detected is placed on one side of a discharge port of the piston cylinder, the driving assembly drives the transmission assembly to operate, the transmission assembly drives the piston block to move, and the piston block is connected with the piston cylinder. When the piston block moves downwards in the piston cavity, the reagent in the piston cavity can be extruded to be quantitatively discharged from the discharging port, the piston block moves downwards intermittently in the process, the displacement is uniform each time, and the amount of the reagent dropwise added each time is certain; the situation that the detection result is affected by too much or too little single-time dropwise adding when a piston at one end of a connecting rod is manually pushed to move is avoided, after the reagent in a piston cylinder is used up, a piston block moves upwards, negative pressure is generated in the piston cylinder, then the reagent stored in a shell is sucked into the piston cylinder through a connecting pipe, and therefore the detection accuracy is improved. Therefore, feeding in the piston barrel is achieved, and convenience and high efficiency are achieved.

Description

Technical Field

[0001] The utility model relates to the technical field of detection instruments, in particular to a reagent dropping device for fluorescence detection. Background Art

[0002] Fluorescence immunoassay technology has the advantages of strong specificity, high sensitivity, and good practicality. Therefore, it is used to measure biologically active compounds with very low content, such as proteins (enzymes, receptors, antibodies), hormones (steroids, thyroid hormones, phthalosteroid hormones), drugs and microorganisms. When performing fluorescence immunoassay, it is necessary to drip reagents such as proteins, hormones, drugs and microorganisms to achieve the purpose of extracting a small amount of reagents for fluorescence immunoassay by dripping. However, when dripping reagents such as proteins, hormones, drugs and microorganisms, traditional reagent dripping devices for fluorescence immunoassay cannot accurately quantify the dripped reagents. Too much reagent dripping will lead to waste of reagents, and too little reagent dripping will lead to inaccurate test results. Therefore, it is necessary to design a reagent dripping device for fluorescence detection to solve this problem. Utility Model Content

[0003] The purpose of the utility model is to provide a reagent dropping device for fluorescence detection to solve the problems raised in the above background technology.

[0004] In order to achieve the above purpose, the utility model provides the following technical solutions:

[0005] A reagent dropping device for fluorescence detection includes a support plate and:

[0006] A mounting frame, the mounting frame is mounted on the support plate, a mounting rod is mounted on the mounting frame, a suspension is mounted on one end of the mounting rod away from the mounting frame, a piston cylinder is mounted on the suspension, and a scale line is set on the outer wall of the piston cylinder;

[0007] A piston block is slidably mounted in the piston cylinder and fits against the inner wall of the piston cylinder;

[0008] a shell, wherein a reagent is stored in the shell;

[0009] A connecting pipe, one end of which is connected to the piston cylinder, and the other end is connected to the housing, and a one-way valve is provided at the connecting pipe and the discharge port of the piston cylinder;

[0010] A drive assembly, wherein the drive assembly is mounted on a mounting frame;

[0011] A transmission assembly, one end of which is connected to the driving assembly, and the other end of which is connected to the piston block.

[0012] As a further solution of the utility model: the drive assembly comprises:

[0013] A driving member, wherein the driving member is mounted on the mounting frame;

[0014] A driving shaft is connected to the output end of the driving member, and the driving shaft is rotatably connected to the mounting frame.

[0015] As a further solution of the utility model: the transmission assembly includes:

[0016] A driven shaft, the driven shaft is rotatably connected to the mounting frame, a driven gear is mounted on the driven shaft, a half gear is meshed on one side of the driven gear, and the half gear is mounted on the driving shaft;

[0017] A threaded rod, one end of which is fixedly connected to an end of the driven shaft away from the mounting frame, a threaded block is threadedly connected to the threaded rod, a guide rod is installed on one side of the threaded block, a guide groove is provided on the side wall of the mounting frame, and an end of the guide rod away from the threaded block is slidably connected to the guide groove;

[0018] A connecting rod, one end of which is connected to the threaded block, and the other end of which is fixedly connected to the piston block.

[0019] As a further solution of the utility model: the driving component is a stepping motor.

[0020] As a further solution of the utility model: a limiting block is installed at one end of the threaded rod away from the driven shaft.

[0021] Compared with the prior art, the utility model has the following beneficial effects: the sample to be tested is placed on one side of the discharge port of the piston cylinder, the driving member drives the driving shaft connected thereto to rotate, the driving shaft drives the half gear to rotate, the half gear rotates and then meshes with the driven gear on one side to drive the driven shaft connected to the driven gear to rotate, the driven shaft rotates and drives the threaded rod to rotate, the threaded rod rotates and drives the threaded block to move under the action of the thread, the threaded block moves and drives the piston block to move through the connecting rod, the reagent in the piston cylinder drips from the discharge port of the piston cylinder by squeezing the piston block, and the intermittent meshing of the half gear and the driven gear ensures the smooth flow of the reagent. Ensure that the displacement of the piston block is uniform each time the connecting rod pushes it, so that the amount of reagent added each time is constant, and avoid manually pushing the piston at one end of the connecting rod to move, resulting in too much or too little single drop of reagent when dropping, which affects the test result. After the reagent in the piston cylinder is added, the installed drive member drives the drive shaft to reverse, and then under the transmission action, the threaded rod drives the threaded block to move up, and the upward movement of the threaded block drives the piston block at one end of the connecting rod to move up. The upward movement of the piston block causes negative pressure to be generated in the piston cylinder, and then the reagent stored in the shell is sucked into the piston cylinder through the connecting pipe, so as to realize feeding in the piston cylinder, which is convenient and efficient. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] In order to more clearly illustrate the specific implementation methods of the utility model or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some implementation methods of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0023] Figure 1 The figure is a schematic diagram of the structure of a reagent dropping device for fluorescence detection.

[0024] Figure 2 A cross-sectional view of the piston cylinder.

[0025] Figure 3 Schematic diagram of the meshing between the half gear and the driven gear.

[0026] Reference numerals:

[0027] 1. Mounting frame; 2. Driving part; 3. Mounting rod; 4. Suspension; 5. Piston cylinder; 6. Scale line; 7. Housing; 8. Connecting pipe; 9. One-way valve; 10. Driving shaft; 11. Half gear; 12. Driven gear; 13. Driven shaft; 14. Threaded block; 15. Connecting rod; 16. Threaded rod; 17. Guide rod; 18. Piston block; 19. Limit block. DETAILED DESCRIPTION

[0028] The technical solution of the utility model will be described clearly and completely below in conjunction with the accompanying drawings. Obviously, the described embodiments are part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0029] In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", and "third" are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance.

[0030] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installation", "connection" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0031] The present invention will be further explained below in conjunction with specific implementation methods.

[0032] See also Figures 1 to 3 As an embodiment of the utility model, a fluorescence detection reagent dropping device includes a support plate and also includes:

[0033] A mounting frame 1, the mounting frame 1 is mounted on a support plate, a mounting rod 3 is mounted on the mounting frame 1, a suspension 4 is mounted on one end of the mounting rod 3 away from the mounting frame 1, a piston cylinder 5 is mounted on the suspension 4, and a scale line 6 is arranged on the outer wall of the piston cylinder 5;

[0034] The piston block 18 is slidably mounted in the piston cylinder 5 and fits against the inner wall of the piston cylinder 5;

[0035] A housing 7, wherein a reagent is stored in the housing 7;

[0036] A connecting pipe 8, one end of which is connected to the piston cylinder 5, and the other end is connected to the housing 7, and a one-way valve 9 is provided at the discharge port of the connecting pipe 8 and the piston cylinder 5;

[0037] A driving assembly, the driving assembly is mounted on the mounting frame 1;

[0038] A transmission assembly, one end of which is connected to the driving assembly, and the other end of which is connected to the piston block 18.

[0039] In this embodiment, when the device is in use, the sample to be tested is placed on one side of the discharge port of the piston cylinder 5, and the transmission component is driven to operate by the installed driving component, and the transmission component drives the piston block 18 to move. When the piston block 18 moves downward in the piston cavity, it will squeeze the reagent in the piston cavity and discharge it quantitatively from the discharge port. The piston block 18 moves down intermittently in this process, and each displacement is uniform, so that the amount of reagent added each time is constant, so as to avoid manually pushing the piston at one end of the connecting rod 15 to move, resulting in excessive or insufficient single drop of reagent when dropping, which affects the test result. The set scale line 6 can be used for observation, which effectively improves the detection accuracy of fluorescent immunoassay. After the reagent in the piston cylinder 5 is used up, under the action of the driving component, the transmission component drives the piston block 18 to move up, and the upward movement of the piston block 18 generates negative pressure in the piston cylinder 5, and then the reagent stored in the shell 7 is sucked into the piston cylinder 5 through the connecting pipe 8, so as to realize feeding into the piston cylinder 5, which is convenient and efficient.

[0040] As an embodiment of the present utility model, the drive assembly comprises:

[0041] A driving member 2, the driving member 2 is mounted on the mounting frame 1;

[0042] The driving shaft 10 is connected to the output end of the driving member 2 , and the driving shaft 10 is rotatably connected to the mounting bracket 1 .

[0043] In this embodiment, the driving member 2 drives the driving shaft 10 connected thereto to rotate, and the rotation of the driving shaft 10 drives the transmission assembly to operate, and the transmission assembly drives the piston block 18 connected thereto to move. When the piston block 18 moves downward in the piston cavity, it squeezes the reagent in the piston cavity and discharges it quantitatively from the discharge port to ensure uniform dripping and use of the reagent. After the reagent in the piston cylinder 5 is used up, the transmission assembly drives the piston block 18 to move upward, and the upward movement of the piston block 18 causes negative pressure to be generated in the piston cylinder 5, and then the reagent stored in the shell 7 is sucked into the piston cylinder 5 through the connecting tube 8, so as to realize feeding into the piston cylinder 5, which is convenient and efficient.

[0044] As an embodiment of the present utility model, the transmission assembly includes:

[0045] A driven shaft 13, the driven shaft 13 is rotatably connected to the mounting frame 1, a driven gear 12 is mounted on the driven shaft 13, a half gear 11 is meshed with one side of the driven gear 12, and the half gear 11 is mounted on the driving shaft 10;

[0046] A threaded rod 16, one end of which is fixedly connected to the end of the driven shaft 13 away from the mounting frame 1, a threaded block 14 is threadedly connected to the threaded rod 16, a guide rod 17 is installed on one side of the threaded block 14, a guide groove is provided on the side wall of the mounting frame 1, and the end of the guide rod 17 away from the threaded block 14 is slidably connected to the guide groove;

[0047] A connecting rod 15 , one end of which is connected to the threaded block 14 , and the other end of which is fixedly connected to the piston block 18 .

[0048] In the present embodiment, the driving shaft 10 rotates to drive the half gear 11 to rotate, the half gear 11 rotates and then meshes with the driven gear 12 on one side to drive the driven shaft 13 connected to the driven gear 12 to rotate, the driven shaft 13 rotates to drive the threaded rod 16 to rotate, the threaded rod 16 rotates to drive the threaded block 14 to move under the action of the thread, the threaded block 14 moves through the connecting rod 15 to drive the piston block 18 to move, and the reagent in the piston cylinder 5 drips from the discharge port of the piston cylinder 5 by squeezing the piston block 18, and the intermittent meshing of the half gear 11 and the driven gear 12 ensures that the connecting rod 15 pushes the piston block 18 to move uniformly each time, so that the amount of reagent added each time is constant, and it is avoided that the piston at one end of the connecting rod 15 is manually pushed to move, resulting in excessive or insufficient single dripping of the reagent when dripping, thereby affecting the detection result.

[0049] Furthermore, after the reagent in the piston cylinder 5 is dripped, the installed driving member 2 drives the driving shaft 10 to reverse, and then the threaded rod 16 drives the threaded block 14 to move upward under the transmission action, and the upward movement of the threaded block 14 drives the piston block 18 at one end of the connecting rod 15 to move upward, and the upward movement of the piston block 18 causes negative pressure to be generated in the piston cylinder 5, and then the reagent stored in the shell 7 is sucked into the piston cylinder 5 through the connecting tube 8, so as to realize the feeding of the piston cylinder 5, which is convenient and efficient.

[0050] As an embodiment of the present invention, the driving member 2 is a stepping motor.

[0051] In this embodiment, the driving member 2 is a stepper motor, which can drive the driving shaft 10 to rotate. The rotation of the driving shaft 10 drives the half gear 11 to rotate. The half gear 11 rotates and then engages with the driven gear 12 on one side to drive the driven shaft 13 connected to the driven gear 12 to rotate.

[0052] As an embodiment of the present invention, a limiting block 19 is installed at one end of the threaded rod 16 away from the driven shaft 13 .

[0053] In this embodiment, a limit block 19 is installed at one end of the threaded rod 16 away from the driven shaft 13. The limit block 19 can limit the threaded block 14 connected to the threaded rod 16 by threads, and the limit block 19 is separated from the threaded rod 16.

[0054] The working principle of the utility model is as follows: when the device is in use, the sample to be tested is placed on one side of the discharge port of the piston cylinder 5, the driving member 2 drives the driving shaft 10 connected thereto to rotate, the driving shaft 10 rotates to drive the half gear 11 to rotate, the half gear 11 rotates and then meshes with the driven gear 12 on one side to drive the driven shaft 13 connected to the driven gear 12 to rotate, the driven shaft 13 rotates to drive the threaded rod 16 to rotate, the threaded rod 16 rotates to drive the threaded block 14 to move under the action of the thread, the threaded block 14 moves through the connecting rod 15 to drive the piston block 18 to move, and the reagent in the piston cylinder 5 drips from the discharge port of the piston cylinder 5 by squeezing the piston block 18, and the reagent in the piston cylinder 5 drips from the discharge port of the piston cylinder 5 through the intermittent rotation of the half gear 11 and the driven gear 12. The piston block 18 is meshed with each other to ensure that the displacement of the piston block 18 is uniform each time the connecting rod 15 pushes it, so that the amount of reagent added each time is constant, and it is avoided that the piston at one end of the connecting rod 15 is manually pushed to move, resulting in too much or too little single drop of reagent when dropping, which affects the test result. After the reagent in the piston cylinder 5 is added, the installed driving member 2 drives the driving shaft 10 to reverse, and then the threaded rod 16 drives the threaded block 14 to move up under the transmission action, and the upward movement of the threaded block 14 drives the piston block 18 at one end of the connecting rod 15 to move up. The upward movement of the piston block 18 generates negative pressure in the piston cylinder 5, and then the reagent stored in the shell 7 is sucked into the piston cylinder 5 through the connecting tube 8, so as to realize feeding into the piston cylinder 5, which is convenient and efficient.

[0055] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the utility model, rather than to limit it. Although the utility model has been described in detail with reference to the aforementioned embodiments, ordinary technicians in this field should understand that they can still modify the technical solutions recorded in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not make the essence of the corresponding technical solution deviate from the scope of the technical solution of the embodiments of the utility model.

Claims

1. A reagent dropping device for fluorescence detection, comprising a support plate, characterized in that: Also includes: A mounting frame, the mounting frame is mounted on the support plate, a mounting rod is mounted on the mounting frame, a suspension is mounted on one end of the mounting rod away from the mounting frame, a piston cylinder is mounted on the suspension, and a scale line is set on the outer wall of the piston cylinder; A piston block is slidably mounted in the piston cylinder and fits against the inner wall of the piston cylinder; a shell, wherein a reagent is stored in the shell; A connecting pipe, one end of which is connected to the piston cylinder, and the other end is connected to the housing, and a one-way valve is provided at the connecting pipe and the discharge port of the piston cylinder; A drive assembly, wherein the drive assembly is mounted on a mounting frame; A transmission assembly, one end of which is connected to the driving assembly, and the other end of which is connected to the piston block.

2. A fluorescence detection reagent dropping device according to claim 1, characterized in that: The drive assembly comprises: A driving member, wherein the driving member is mounted on the mounting frame; A driving shaft is connected to the output end of the driving member, and the driving shaft is rotatably connected to the mounting frame.

3. A fluorescence detection reagent dropping device according to claim 2, characterized in that: The transmission assembly comprises: A driven shaft, the driven shaft is rotatably connected to the mounting frame, a driven gear is mounted on the driven shaft, a half gear is meshed on one side of the driven gear, and the half gear is mounted on the driving shaft; A threaded rod, one end of which is fixedly connected to an end of the driven shaft away from the mounting frame, a threaded block is threadedly connected to the threaded rod, a guide rod is installed on one side of the threaded block, a guide groove is provided on the side wall of the mounting frame, and an end of the guide rod away from the threaded block is slidably connected to the guide groove; A connecting rod, one end of which is connected to the threaded block, and the other end of which is fixedly connected to the piston block.

4. A fluorescence detection reagent dropping device according to claim 2, characterized in that: The driving component is a stepping motor.

5. A fluorescence detection reagent dropping device according to claim 3, characterized in that: A limiting block is installed at one end of the threaded rod away from the driven shaft.

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