SPE column using device

Abstract

The utility model discloses an SPE (Solid Phase Extraction) column using device and relates to the technical field of solid phase extraction. An SPE column using device comprises a column inserting pipe, a column connecting pipe, a column connecting pipe and a column connecting pipe, wherein an adjusting valve is arranged on the column inserting pipe; the connecting assembly comprises a screw cap and a pipe body detachably connected with the screw cap; the column insertion pipe is inserted into the screw cap in a penetrating manner; an air guide pipe is arranged on the peripheral side of the pipe wall of the pipe body; the centrifugal tube connecting assembly comprises a first connecting tube and a centrifugal tube; one side of the first connecting tube is detachably connected with the lower end of the tube body, and the other side is detachably connected with the centrifugal tube; and the flow guide pipe is inserted into the lower pipe orifice of the column insertion pipe and is positioned in the pipe body. The utility model designs a novel SPE column using device, and the SPE column using device is small in occupied space, easy to store, low in manufacturing cost and more suitable for processing a single sample.

Description

Technical Field

[0001] The utility model relates to the technical field of solid phase extraction, in particular to a SPE column using device. Background Art

[0002] A solid phase extraction column (SPE column) is a sample pretreatment device for extraction, separation, and concentration. When the SPE column is used, the sample passes through the solid phase filler in the SPE column, and with the help of negative pressure extraction equipment, the sample quickly passes through the filler layer, so that the target analyte effectively reacts with the solid phase filler for sample separation and extraction.

[0003] At present, SPE columns are used with the aid of a solid phase extraction device. Common solid phase extraction devices generally include a transparent square container and a cover fixed on the top of the square container. Multiple SPE column plug-in interfaces are provided on the top of the cover for plugging in multiple SPE columns in the same batch, and a negative pressure device is connected to the square container for extraction operations.

[0004] Regarding the above common solid phase extraction device, the inventors believe that: the above solid phase extraction device occupies a large space and has a high cost; and the above solid phase extraction device is generally more suitable for batch processing of samples. If only a single sample is processed, it will lead to a waste of resources of the solid phase extraction device.

[0005] In this regard, the inventors believe that how to design an SPE column usage device that occupies a small space and is easy to store, has a low cost, and is more suitable for processing a single sample is a technical problem that the technicians in this field urgently need to solve.

[0006] The information disclosed in this background technology section is only intended to increase the understanding of the overall background of the present invention, and should not be regarded as acknowledging or suggesting in any form that the information constitutes the prior art already known to a person skilled in the art. Utility Model Content

[0007] In view of the above technical problems, an embodiment of the utility model provides a SPE column using device to solve the problems raised in the above background technology.

[0008] The utility model provides the following technical solutions:

[0009] A device for using an SPE column, comprising:

[0010] A column cannula, on which a regulating valve is arranged;

[0011] The connecting assembly comprises a screw cap and a tube body detachably connected to the screw cap; the column insert is inserted through the screw cap; and an air guide tube is arranged on the peripheral side of the tube wall of the tube body;

[0012] A centrifuge tube connection assembly, comprising a first connection tube and a centrifuge tube; one side of the first connection tube is detachably connected to the lower end of the tube body, and the other side of the first connection tube is detachably connected to the centrifuge tube;

[0013] The flow guide pipe is plugged into the lower pipe opening of the column insert pipe and is located inside the pipe body.

[0014] Preferably, a pump tube interface is provided on the peripheral side of the tube wall of the tube body, and a sealing cover is connected to the pump tube interface.

[0015] Preferably, a first internal thread is provided on the inner circumferential wall of the screw cap, a first external thread is provided on the upper pipe opening of the tube body, and a second internal thread is provided on the lower pipe opening of the tube body; the tube body is screwed onto the first internal thread of the inner circumferential wall of the screw cap through the first external thread of the upper pipe opening.

[0016] Preferably, the first connecting pipe is a straight pipe structure; a second external thread is provided on the upper pipe opening of the first connecting pipe, and a third internal thread is provided on the lower pipe opening of the first connecting pipe; the first connecting pipe is screwed to the second internal thread of the lower pipe opening of the tube body through the second external thread of the upper pipe opening.

[0017] Preferably, the first connecting tube can be replaced by a second connecting tube; the second connecting tube includes a straight tube and a cover body fixedly connected to the bottom of the straight tube; a fourth external thread is provided on the upper tube opening of the straight tube, and a fourth internal thread is provided on the peripheral wall on the outer side of the cover body; the second connecting tube is screwed onto the second internal thread of the lower tube opening of the tube body through the fourth external thread of the upper tube opening.

[0018] Preferably, a third external thread is provided on the pipe mouth of the centrifuge tube; the centrifuge tube is screwed onto the third internal thread of the lower pipe mouth of the first connecting tube through the third external thread of the pipe mouth.

[0019] Preferably, a third external thread is provided on the pipe mouth of the centrifuge tube; the centrifuge tube is screwed onto the fourth internal thread of the second connecting tube through the third external thread of the pipe mouth.

[0020] An SPE column using device provided by an embodiment of the utility model has the following beneficial effects: The utility model designs a novel SPE column using device, which occupies a small space, is easy to store, has a low cost, and is more suitable for processing a single sample. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 This is a structural schematic diagram of the utility model from angle 1;

[0022] Figure 2 It is a perspective view of the utility model from angle 2;

[0023] Figure 3 It is an exploded view of the utility model;

[0024] Figure 4 This is a structural schematic diagram of the connection between the central column intubation, the regulating valve and the rotary cover of the utility model;

[0025] Figure 5 It is a structural schematic diagram of the tube body in the utility model;

[0026] Figure 6 It is a structural schematic diagram of the second connecting pipe in the utility model. DETAILED DESCRIPTION

[0027] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by those skilled in the art without creative work are within the scope of protection of the utility model.

[0028] See also Figure 1-Figure 6 .

[0029] In view of the problems mentioned in the above background technology, the embodiment of the present utility model provides a SPE column using device to solve the above technical problems, and its technical solution is as follows:

[0030] A device for using an SPE column, comprising:

[0031] The column insert 130 is provided with a regulating valve 131; the regulating valve 131 is used for adjusting the flow rate;

[0032] The connection assembly includes a rotary cover 110 and a tube body 120 detachably connected to the rotary cover 110; in order to improve the sealing performance of the connection between the tube body 120 and the rotary cover 110, a sealing gasket may be provided at the contact position between the bottom wall of the rotary cover 110 and the tube body 120;

[0033] The column insert 130 is inserted through the screw cap 110; an air guide tube 121 is provided on the peripheral side of the tube wall of the tube body 120; the air guide tube 121 is connected to the inside of the tube body 120, and the air guide tube 121 can be connected to a syringe or a small mechanical pump during later use to create negative pressure conditions;

[0034] The centrifuge tube connection assembly includes a first connection tube 140 and a centrifuge tube 150; one side of the first connection tube 140 is detachably connected to the lower end of the tube body 120, and the other side thereof is detachably connected to the centrifuge tube 150;

[0035] The flow guide tube 160 is inserted into the lower tube opening of the column insert tube 130 and is located in the tube body 120 ; the flow guide tube 160 is used to guide the sample solution after extraction and filtration, so as to facilitate the collection of the sample into the centrifuge tube 150 .

[0036] The working method of an SPE column using device provided by the embodiment of the utility model is as follows:

[0037] 1. Device assembly:

[0038] (1) Insert the column insert tube 130 through the screw cap 110, and insert the flow guide tube 160 into the lower tube opening of the column insert tube 130;

[0039] (2) Connect the tube body 120 below the screw cap 110, connect the first connecting tube 140 below the tube body 120, and connect the centrifuge tube 150 below the first connecting tube 140;

[0040] (3) Connecting a syringe or a small mechanical pump to the airway tube 121;

[0041] 2. Install the SPE column:

[0042] Insert the SPE column into the upper tube opening of the column insert tube 130;

[0043] 3. Sample extraction and separation:

[0044] Pour the sample into the SPE column, adjust the regulating valve 131 to adjust the flow rate, operate the syringe or a small mechanical pump to create a negative pressure environment, and collect the extracted and separated sample into the centrifuge tube 150.

[0045] In this embodiment, a pump pipe interface 122 is provided on the peripheral side of the pipe wall of the pipe body 120, and a sealing cover 170 is connected to the pump pipe interface 122;

[0046] If small batch sample processing is required, multiple SPE column using devices can be used at the same time, and each SPE column using device can be separately plugged into an SPE column; remove the sealing cover 170 on the pump tube interface 122, and connect each pump tube interface separately through multiple branch pipelines, and the multiple branch pipelines are connected through a main pipeline, and a mechanical pump is connected to the main pipeline for batch sample processing.

[0047] It should be noted that when processing small batches of samples as described above, the air guide tube 121 on the tube body 120 needs to be sealed, and the air guide tube 121 can be separately provided with a sealing cover or sealed with a sealing tape.

[0048] In this embodiment, a first internal thread 111 is provided on the inner peripheral wall of the screw cap 110, a first external thread 123 is provided on the upper pipe opening of the tube body 120, and a second internal thread 124 is provided on the lower pipe opening of the tube body 120; the tube body 120 is screwed to the first internal thread 111 of the inner peripheral wall of the screw cap 110 through the first external thread 123 of the upper pipe opening; the above structural design realizes a detachable connection between the tube body 120 and the screw cap 110.

[0049] In this embodiment, the first connecting tube 140 is a straight tube structure; the upper tube opening of the first connecting tube 140 is provided with a second external thread 141, and the lower tube opening of the first connecting tube 140 is provided with a third internal thread 142; the first connecting tube 140 is screwed to the second internal thread 124 of the lower tube opening of the tube body 120 through the second external thread 141 of the upper tube opening; the above structural design realizes the detachable connection between the first connecting tube 140 and the tube body 120;

[0050] At the same time, a third external thread 151 is provided on the pipe mouth of the centrifuge tube 150; the centrifuge tube 150 is screwed onto the third internal thread 142 of the lower pipe mouth of the first connecting tube 140 through the third external thread 151 of the pipe mouth; the above structural design facilitates the detachable connection of the centrifuge tube 150 to the first connecting tube 140.

[0051] In this embodiment, the first connecting tube 140 can be replaced by a second connecting tube 180; the second connecting tube 180 includes a straight tube 181 and a cover 182 fixedly connected to the bottom of the straight tube 181; a fourth external thread 183 is provided on the upper tube opening of the straight tube 181, and a fourth internal thread 184 is provided on the peripheral wall outside the cover 182; the second connecting tube 180 is screwed to the second internal thread 124 of the lower tube opening of the tube body 120 through the fourth external thread 183 of the upper tube opening;

[0052] Meanwhile, a third external thread 151 is provided on the pipe mouth of the centrifugal tube 150 ; the centrifugal tube 150 is screwed onto the fourth internal thread 184 of the second connecting tube 180 via the third external thread 151 of the pipe mouth.

[0053] It should be noted that when the first connecting tube 140 is assembled on the SPE column using device, the first connecting tube 140 is suitable for connecting a centrifuge tube 150 of small volume (such as 15ml); if the amount of sample to be extracted and collected is large, a centrifuge tube 150 of large volume (such as 50ml) is required. At this time, the first connecting tube 140 needs to be replaced with the second connecting tube 180. The large-size connection position below the second connecting tube 180 is suitable for installing a centrifuge tube 150 of larger volume.

[0054] In this embodiment, the pipes on the SPE column device are mostly connected by threads. In order to improve the sealing performance of the threaded connection, an O-ring is generally installed between the two pipes for sealing connection.

[0055] In this embodiment, all components are made of high-quality polypropylene, which is easy to disassemble, store, clean and replace.

[0056] An SPE column using device provided by an embodiment of the utility model has the following beneficial effects: The utility model designs a novel SPE column using device, which occupies a small space, is easy to store, has a low cost, and is more suitable for processing a single sample.

[0057] The device embodiments described above are merely illustrative, wherein the units described as separate components may or may not be physically separated, and the components shown as units may or may not be physical units, and may be located in one place or distributed over multiple network units. Some or all of the modules may be selected according to actual needs to achieve the purpose of the solution of this embodiment.

[0058] In the present utility model, unless otherwise clearly stipulated and limited, the terms such as "installation", "setting", "connection", "fixation" and "screw-on" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integrated connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. Unless otherwise clearly defined, ordinary technicians in this field can understand the specific meanings of the above terms in the utility model according to the specific circumstances.

[0059] It is understandable that those skilled in the art can make equivalent substitutions or changes based on the technical solution and concept of the utility model, and all these changes or substitutions should fall within the protection scope of the claims attached to the utility model.

Claims

1. A device for using an SPE column, characterized in that: include: A column cannula, on which a regulating valve is arranged; The connecting assembly comprises a screw cap and a tube body detachably connected to the screw cap; the column insert is inserted through the screw cap; and an air guide tube is arranged on the peripheral side of the tube wall of the tube body; A centrifuge tube connection assembly, comprising a first connection tube and a centrifuge tube; one side of the first connection tube is detachably connected to the lower end of the tube body, and the other side of the first connection tube is detachably connected to the centrifuge tube; The flow guide pipe is plugged into the lower pipe opening of the column insert pipe and is located inside the pipe body.

2. The SPE column using device according to claim 1, characterized in that, A pump pipe interface is arranged on the peripheral side of the pipe wall of the pipe body, and a sealing cover is connected to the pump pipe interface.

3. The SPE column using device according to claim 1, characterized in that, A first internal thread is arranged on the inner peripheral wall of the screw cap, a first external thread is arranged on the upper pipe opening of the tube body, and a second internal thread is arranged on the lower pipe opening of the tube body; the tube body is screwed onto the first internal thread of the inner peripheral wall of the screw cap through the first external thread of the upper pipe opening.

4. The SPE column using device according to claim 3, characterized in that, The first connecting pipe is a straight pipe structure; a second external thread is arranged on the upper pipe opening of the first connecting pipe, and a third internal thread is arranged on the lower pipe opening of the first connecting pipe; the first connecting pipe is screwed to the second internal thread of the lower pipe opening of the tube body through the second external thread of the upper pipe opening.

5. The SPE column using device according to claim 3, characterized in that, The first connecting tube can be replaced by a second connecting tube; the second connecting tube includes a straight tube and a cover body fixedly connected to the bottom of the straight tube; a fourth external thread is provided on the upper tube opening of the straight tube, and a fourth internal thread is provided on the peripheral wall outside the cover body; the second connecting tube is screwed onto the second internal thread of the lower tube opening of the tube body through the fourth external thread of the upper tube opening.

6. The SPE column using device according to claim 4, characterized in that, The centrifuge tube is provided with a third external thread on the tube mouth; the centrifuge tube is screwed onto the third internal thread on the lower tube mouth of the first connecting tube through the third external thread on the tube mouth.

7. The SPE column using device according to claim 5, characterized in that, The centrifuge tube is provided with a third external thread on the tube mouth; the centrifuge tube is screwed onto the fourth internal thread of the second connecting tube through the third external thread on the tube mouth.

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