Equipment for detecting shigella in food

Abstract

The utility model belongs to the field of detection, particularly relates to equipment for detecting shigella in food, and provides the following scheme aiming at the problems that although a shell can be adsorbed on a plane through a sucking disc, a plurality of samples to be detected cannot be placed, and a test tube rack needs to be additionally taken for placing, so that the operation is relatively inconvenient. The device comprises a PCR instrument main body used for detecting shigella in food and a test tube filled with a food sample, supporting legs used for supporting the PCR instrument main body are fixedly arranged at four corners of the bottom of the PCR instrument main body, and anti-skid rubber pads are fixedly arranged at the bottoms of the supporting legs. The placing mechanism is used for placing a sample test tube to be detected, so that the test tube rack does not need to be additionally taken, the operation is more convenient, the PCR instrument main body can be adsorbed and fixed on a table top through the fixing mechanism, and sliding is prevented.

Description

Technical Field

[0001] The utility model relates to the technical field of detection, in particular to Shigella detection equipment in food. Background Art

[0002] Food inspection in a broad sense refers to a discipline that studies and evaluates food quality and its changes. It is based on some basic theories and various technologies of physics, chemistry, and biochemistry, and in accordance with established technical standards, such as international and national food hygiene / safety standards, to inspect the quality of food raw materials, auxiliary materials, semi-finished products, finished products, and by-products to ensure that the product quality is qualified. The content of food inspection includes sensory testing of food, testing of nutrients, additives, and harmful substances in food, etc. The content of food inspection is very rich, including analysis of food nutrients, analysis of pollutants in food, analysis of food auxiliary materials and food additives, food sensory identification, etc. Food inspection in a narrow sense is usually an inspection of food quality, including inspection of the characteristics of food outer packaging, inner packaging, signs, marks, and appearance of the commodity, physical and chemical indicators, and some other hygiene indicators. Shigella is an item that needs to be tested in food inspection.

[0003] Detection of Shigella in food usually requires microbiological testing. The following are some common methods and equipment used to detect Shigella in food:

[0004] PCR machine: PCR (polymerase chain reaction) is a molecular biology technique that can detect Shigella DNA in food samples. PCR machine can be used to confirm the presence of Shigella by detecting the DNA sequence.

[0005] Media and Petri dishes: Media is a culture medium containing specific nutrients and additives used to grow Shigella in the laboratory. Food samples are spread on petri dishes containing appropriate media to encourage Shigella to grow in the presence or absence of oxygen.

[0006] Immunological test kits: Immunological test kits use antibodies or antigens that bind to specific Shigella proteins to determine if the bacteria is present in a food sample. This method can detect Shigella quickly and accurately.

[0007] Mass spectrometers: Mass spectrometers can be used to analyze the protein composition of bacteria in food samples, thereby identifying Shigella and helping to confirm its presence.

[0008] Rapid Test Strips: Several commercial rapid test strips are available for testing food samples for Shigella. These strips are quick and easy to identify the presence of Shigella.

[0009] PCR is the use of DNA polymerase to synthesize a large number of specific genes in vitro or in vitro. Basically, it uses DNA polymerase to perform specific chain replication. The commonly used technology can replicate a gene to 10 billion to 100 billion times the original. According to the purpose of DNA amplification and the standard of detection, PCR instruments can be divided into four categories: ordinary PCR instrument, gradient PCR instrument, in situ PCR instrument, and real-time fluorescence quantitative PCR instrument.

[0010] After searching, the patent with announcement number CN219670511U discloses a fluorescent quantitative PCR instrument, which includes a shell and a heating chamber, the bottom of the inner cavity of the shell is provided with a PCR instrument body, the top of the shell is provided with a groove, a placement rack is provided in the groove, the placement rack is provided with a socket, a reaction tube is provided in the socket, the heating chamber is provided at the bottom of the placement rack, and the reaction tube is embedded in the heating chamber. In the application document, during the use of the fluorescent quantitative PCR instrument, it is convenient to quickly cool the reaction tube, the heat dissipation effect is good, and it is convenient to quickly take out the reaction tube for the next experiment, saving waiting time and improving work efficiency.

[0011] The technical solution has the following problems:

[0012] Although the shell can be adsorbed on a flat surface by a suction cup, multiple samples to be tested cannot be placed on it, and an additional test tube rack is required for placement, which is inconvenient. Utility Model Content

[0013] The purpose of the utility model is to solve the disadvantages of the prior art that although the shell can be adsorbed on a plane by a suction cup, multiple samples to be tested cannot be placed and an additional test tube rack is required for placement, which is inconvenient. The Shigella detection device in food is proposed.

[0014] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0015] The Shigella detection device in food comprises a PCR instrument body for detecting Shigella in food and a test tube containing a food sample, wherein four corners of the bottom of the PCR instrument body are fixedly provided with supporting feet for supporting the PCR instrument body, and the bottom of the supporting feet is fixedly provided with a rubber pad for anti-slip;

[0016] A placing mechanism, which is arranged on one side of the PCR instrument body and is used to place a test tube containing a sample to be tested;

[0017] The fixing mechanism is arranged on the PCR instrument body and is used to fix the PCR instrument body.

[0018] In a possible design, the placement mechanism includes a mounting frame, a test tube rack for placing test tubes, and a placement box for placing the test tube rack. Four corners of one side of the mounting frame are fixedly connected to one side of the PCR instrument body, one side of the placement box is fixedly connected to one side of the mounting frame, the test tube rack is placed in the placement box, the test tube is placed in the test tube rack, the test tube rack is adapted to the test tube, and one side of the placement box is hinged with an upper cover via a hinge.

[0019] In a possible design, the fixing mechanism includes two groups of short plates, four threaded rods and four suction cups. The two groups of short plates each consist of two short plates. The sides of the two groups of short plates close to each other are respectively fixedly connected to the two sides of the PCR instrument body. The four threaded rods are respectively threadedly connected to the four short plates. The top ends of the four suction cups are respectively fixedly connected to the bottom ends of the four threaded rods. The top ends of the threaded rods are fixedly provided with knobs for facilitating the rotation of the threaded rods.

[0020] In a possible design, a magnet for magnetically fixing the upper cover is fixedly disposed on one side of the top of the placement box, and an iron sheet is fixedly disposed on one side of the bottom of the upper cover.

[0021] In a possible design, the knob is provided with anti-slip grooves for increasing the friction between a person's hand and the knob.

[0022] In a possible design, a lift plate is fixedly provided on one side of the upper cover for facilitating the upper cover to be lifted up and opened.

[0023] In the present application, when in use, first place the PCR instrument body on a flat surface, prop up the PCR instrument body with the legs, then turn the four knobs in sequence, the rotation of the knobs drives the rotation of the threaded rod, the rotation of the threaded rod drives the movement of the suction cup, the suction cup fits the desktop to squeeze out the air in the suction cup, so that the PCR instrument body can be adsorbed and fixed on the desktop to prevent sliding, then place a plurality of test tubes containing samples to be tested in the test tube rack in the placement box, and then close the upper cover, so that the test tubes containing samples to be tested can be placed, and there is no need to take the test tube rack additionally, which is more convenient. The test tube is a test tube with a rubber stopper. Since the test tube rack is placed in the placement box, the corresponding test tube rack can be replaced according to the size of the test tube. When the upper cover is closed on the placement box, the magnet and the iron sheet will contact and attract each other, and the upper cover is magnetically fixed. When the test is performed, the Shigella in the sample in the test tube is detected by the PCR instrument body, wherein the mounting frame is connected to the PCR instrument body through the four corners of the mounting frame, and will not affect the heat dissipation of the PCR instrument body.

[0024] The beneficial effects of the present invention are:

[0025] In the utility model, the Shigella detection device in food, through the placement mechanism, places a plurality of test tubes containing samples to be detected in the test tube rack in the placement box, and then closes the upper cover, so that the test tubes containing samples to be detected can be placed, and there is no need to take the test tube rack additionally, which is more convenient;

[0026] In the utility model, the Shigella detection device in food is used to rotate four knobs in sequence through a fixing mechanism, the rotation of the knobs drives the rotation of the threaded rod, the rotation of the threaded rod drives the movement of the suction cup, the suction cup is attached to the desktop to squeeze out the air in the suction cup, so that the PCR instrument body can be adsorbed and fixed on the desktop to prevent sliding;

[0027] In the utility model, the test tubes containing samples to be tested are placed by means of a placement mechanism, so there is no need to take an additional test tube rack, which is more convenient. The PCR instrument body can be adsorbed and fixed on the desktop by the fixing mechanism to prevent sliding. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 This is a schematic diagram of the main structure of the Shigella detection device in food proposed by the utility model;

[0029] Figure 2 This is a front view structural diagram of the Shigella detection device in food proposed by the utility model;

[0030] Figure 3 This is a side structural schematic diagram of the Shigella detection device in food proposed by the utility model;

[0031] Figure 4 This is a schematic diagram of the structure of the Shigella detection device in food proposed by the utility model without the upper cover installed;

[0032] Figure 5 This is a schematic diagram of the test tube rack structure of the Shigella detection equipment in food proposed by the utility model.

[0033] In the figure: 1. PCR instrument body; 2. Support feet; 3. Short board; 4. Knob; 5. Threaded rod; 6. Suction cup; 7. Placement box; 8. Anti-slip grooves; 9. Mounting frame; 10. Lifting plate; 11. Upper cover; 12. Test tube rack; 13. Magnet; 14. Test tube. DETAILED DESCRIPTION

[0034] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments.

[0035] Embodiment 1

[0036] Reference Figure 1-5 , detection equipment, including: a PCR instrument body 1, used for performing PCR reaction to detect Shigella in food samples, and legs 2 are arranged at the four corners of the bottom of the PCR instrument body 1, which are not only used to support the PCR instrument body 1, but also ensure that it is stably placed on a plane, and rubber pads are fixed at the bottom of the legs 2 to enhance the anti-slip performance of the PCR instrument body 1 when placed, so as to prevent it from sliding or shifting during operation.

[0037] In order to meet the needs of different usage environments and conditions, a placement mechanism is also designed, which includes a mounting frame 9, a test tube rack 12 and a placement box 7. The four corners of one side of the mounting frame 9 are fixedly connected to one side of the PCR instrument body 1, providing stable support for the placement mechanism. One side of the placement box 7 is fixedly connected to one side of the mounting frame 9 to ensure the integrity and stability of the placement mechanism and the PCR instrument body 1. The test tube rack 12 is placed in the placement box 7 to accommodate a test tube 14 containing a food sample. The test tube rack 12 is adapted to the test tube 14 to ensure that the test tube 14 can be stably placed in the test tube rack 12 to prevent movement or tilting. In addition, one side of the placement box 7 is hinged with an upper cover 11 through a hinge to protect the test tube 14 from external contamination when the PCR reaction is not required.

[0038] In order to further enhance the stability of the PCR instrument body 1 when it is placed, a fixing mechanism is also designed, which includes two groups of short plates 3, four threaded rods 5 and four suction cups 6. The two groups of short plates 3 each consist of two short plates 3, and their mutually close sides are fixedly connected to the two sides of the PCR instrument body 1, respectively, to provide a stable installation foundation for the threaded rods 5. The four threaded rods 5 are respectively threadedly connected to the four short plates 3, so that the threaded rods 5 can be adjusted in height as needed. The top ends of the four suction cups 6 are respectively fixedly connected to the bottom ends of the four threaded rods 5. When the PCR instrument body 1 needs to be fixed, it is only necessary to rotate the threaded rod 5 to a suitable height so that the suction cups 6 are tightly fitted with the contact surface, thereby fixing the PCR instrument body 1 in the desired position through the adsorption force of the suction cups 6. In order to facilitate the rotation of the threaded rod 5, a knob 4 is also fixedly provided on the top of the threaded rod 5, and an anti-slip pattern 8 is provided on the knob 4 to increase the friction between the person's hand and the knob 4, making the rotation operation easier and more accurate.

[0039] The present application is used in the field of Shigella detection in food, and can also be used in other fields applicable to the present application.

[0040] The model of the PCR instrument main body 1 is the Thermo real-time fluorescence quantitative PCR instrument QuantStudioTM5 produced by Thermo Fisher Scientific. The PCR instrument main body 1 is the existing technology. The working principle of the PCR instrument main body 1 will not be described here. Of course, it can also be purchased according to actual needs.

[0041] Embodiment 2

[0042] refer to Figure 1-5 , based on the improvement of the first embodiment: a device for detecting Shigella in food, which is used in the field of detecting Shigella in food,

[0043] In order to facilitate the user to open and close the placement box 7, a magnet 13 is provided on the top side of the placement box 7, and an iron sheet is provided on the bottom side of the upper cover 11. When the upper cover 11 is closed, the magnet 13 will attract the iron sheet, so that the upper cover 11 is firmly attached to the placement box 7 to prevent the test tube 14 from being contaminated by the outside world. When the upper cover 11 needs to be opened, the upper cover 11 can be easily opened by gently lifting the lifting plate 10, which is convenient for the user to take and place the test tube 14.

[0044] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes within the technical scope disclosed by the present invention according to the technical scheme and the utility model concept of the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A device for detecting Shigella in food, comprising a PCR instrument body (1) for detecting Shigella in food and a test tube (14) containing a food sample, characterized in that: The four corners of the bottom of the PCR instrument body (1) are fixedly provided with supporting feet (2) for supporting the PCR instrument body (1), and the bottom of the supporting feet (2) is fixedly provided with a rubber pad for anti-slip; A placement mechanism, which is arranged on one side of the PCR instrument body (1) and is used to place a test tube (14) containing a sample to be tested; A fixing mechanism is arranged on the PCR instrument body (1) and is used to fix the PCR instrument body (1).

2. The Shigella detection device in food according to claim 1, characterized in that: The placement mechanism comprises a mounting frame (9), a test tube rack (12) for placing a test tube (14), and a placement box (7) for placing the test tube rack (12); four corners of one side of the mounting frame (9) are fixedly connected to one side of a PCR instrument body (1); one side of the placement box (7) is fixedly connected to one side of the mounting frame (9); the test tube rack (12) is placed in the placement box (7); the test tube (14) is placed in the test tube rack (12); the test tube rack (12) is compatible with the test tube (14); and one side of the placement box (7) is hingedly connected to an upper cover (11) via a hinge.

3. The Shigella detection device in food according to claim 1, characterized in that: The fixing mechanism comprises two groups of short plates (3), four threaded rods (5) and four suction cups (6); each group of the two groups of short plates (3) comprises two short plates (3); the sides of the two groups of short plates (3) close to each other are respectively fixedly connected to the two sides of the PCR instrument body (1); the four threaded rods (5) are respectively threadedly connected to the four short plates (3); the top ends of the four suction cups (6) are respectively fixedly connected to the bottom ends of the four threaded rods (5); and the top ends of the threaded rods (5) are fixedly provided with knobs (4) for facilitating the rotation of the threaded rods (5).

4. The Shigella detection device in food according to claim 2, characterized in that: A magnet (13) for magnetically fixing the upper cover (11) is fixedly arranged on one side of the top of the placement box (7), and an iron sheet is fixedly arranged on one side of the bottom of the upper cover (11).

5. The Shigella detection device in food according to claim 3, characterized in that: The knob (4) is provided with anti-slip grooves (8) for increasing the friction between a person's hand and the knob (4).

6. The Shigella detection device in food according to claim 2, characterized in that: A lifting plate (10) is fixedly provided on one side of the upper cover (11) for facilitating the lifting and opening of the upper cover (11).

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