Stem cell separation and extraction mechanism

Abstract

The utility model discloses a stem cell separation and extraction mechanism, which belongs to the technical field of stem cell extraction and comprises a separation case, a mounting cavity is arranged in the separation case, a groove is arranged on the lower side of the mounting cavity, a motor is arranged in the groove, a centrifugal disc is arranged at the output end of the motor, and a plurality of slots are distributed at the upper end of the centrifugal disc at equal intervals. By arranging the sealing assembly, after a test tube is inserted into the inserting grooves, an electric push rod is started to push a moving plate and a connecting plate to move downwards, the electric push rod is closed after a sealing plug at the lower end of a rotating plate is inserted into the test tube, and when a centrifugal disc rotates, the test tube is separated from the rotating plate, so that the test tube is separated from the rotating plate. The rotating plate rotates at the lower end of the connecting plate through the rotating column, so that the upper end of the test tube is sealed by the sealing plug, the upper end of the test tube can be sealed according to test tubes with different heights by arranging the sealing assembly, and leakage of a solution in the test tube is avoided.

Description

Technical Field

[0001] The utility model belongs to the technical field of stem cell extraction, and in particular relates to a stem cell separation and extraction mechanism. Background Art

[0002] Stem cells are a type of multipotent cells with the ability to self-replicate. Under certain conditions, they can differentiate into multiple functional cells. According to the developmental stage of stem cells, they are divided into embryonic stem cells and adult stem cells. According to the developmental potential of stem cells, they are divided into three categories: totipotent stem cells, pluripotent stem cells and unipotent stem cells (specialized stem cells). Stem cells are a type of immature, underdifferentiated cell that has the potential to regenerate various tissues, organs and the human body. They are called "universal cells" in the medical field.

[0003] Chinese patent application No. 2020222717262 discloses a stem cell separation and extraction device, including a stem cell separation chassis and a case cover rotatably connected to the stem cell separation chassis. The stem cell separation and extraction device is provided with a plurality of slots on the upper surface of a centrifugal disk of a centrifugal mechanism, a rotating shaft is fixedly connected in a through hole in the middle of the centrifugal disk, a threaded rod is threadedly connected in the threaded groove of the rotating shaft, the lower end of the threaded rod is fixedly connected to the output end of the motor, and a cavity is provided on one side of the bottom surface of the centrifugal disk. The entire centrifugal mechanism can be detached from the installation cavity for easy cleaning, and a protective cover is fixedly connected to the upper end of the slot, and two symmetrical springs are fixedly connected to the outside of the protective cover, and the other end of the spring is fixedly connected to the inner wall of the slot, so as to facilitate the fixation of the test tube during the centrifugation process.

[0004] The above-mentioned patent discloses that the test tube is rotated and centrifuged by sealing the upper end of the test tube by means of a box cover. The box cover cannot seal the upper end of the test tube according to the test tubes of different heights, and the upper end of the test tube is prone to wear between the lower end of the box cover. At the same time, the stability of the centrifugal disk of the original device is poor when it is rotated. Utility Model Content

[0005] In order to solve the problems raised in the above background technology, the utility model provides a stem cell separation and extraction mechanism, which has the characteristic of being able to seal the upper ends of test tubes of different heights.

[0006] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a stem cell separation and extraction mechanism, comprising a separation chassis, wherein an installation cavity is arranged in the separation chassis, a groove is arranged at the lower side of the installation cavity, a motor is arranged in the groove, a centrifugal disk is arranged at the output end of the motor, a plurality of slots are evenly distributed at the upper end of the centrifugal disk, a clamping mechanism is arranged inside each of the slots, and a sealing assembly is arranged at the upper end of the separation chassis.

[0007] Preferably, the sealing assembly includes a fixed frame, an electric push rod, a movable plate, a connecting plate, a rotating plate, a rotating column, a sealing plug and a buffer assembly, wherein a fixed frame is arranged at the upper end of the separation chassis, an electric push rod is arranged at the upper end of the fixed frame, a movable plate is arranged at the output end of the electric push rod, a connecting plate is arranged at the lower side of the movable plate, a rotating column is arranged at the lower end of the connecting plate, a rotating plate is arranged at the lower end of the rotating column, a sealing plug is arranged at the position of the lower end of the rotating plate corresponding to the slot, and a buffer assembly is arranged between the connecting plate and the movable plate.

[0008] Preferably, the buffer assembly includes a fixed cylinder, a slide plate, a slide rod and a spring, wherein a plurality of fixed cylinders are arranged on the upper end of the connecting plate, a slide plate is arranged inside the fixed cylinder, the slide plate is connected to the movable plate by a slide rod, and a spring is arranged at the lower end of the slide plate.

[0009] Preferably, the fixing frame and the separation chassis as well as the rotating column and the rotating plate are welded by pressure welding, and adjacent fixing cylinders form a ninety-degree angle.

[0010] Preferably, a bearing rotating seat is provided between the rotating column and the connecting plate, the sealing plug and the rotating plate are bonded by glue, and a sliding hole corresponding to the sliding rod is provided at the upper end of the fixing cylinder.

[0011] Preferably, the centrifugal disc is provided with a plurality of connecting blocks on the side, a folding plate is provided at one end of the connecting block, a roller is provided in the folding plate, an annular plate is provided at a position corresponding to the roller in the mounting cavity, and an annular groove corresponding to the roller is provided on the inner side of the annular plate.

[0012] Preferably, the roller and the folding plate are rotatably connected via an axle pin, the side of the roller is in close contact with the inner wall of the annular groove, and the annular plate and the separation chassis are spot welded.

[0013] Preferably, there is an angle of ninety degrees between adjacent rollers, and the annular plate and the separation chassis are welded by spot welding.

[0014] Compared with the prior art, the beneficial effects of the utility model are:

[0015] 1. The utility model sets a sealing component. After the test tube is inserted into the slot, the electric push rod is turned on to push the movable plate and the connecting plate to move downward. After the sealing plug at the lower end of the rotating plate is inserted into the test tube, the electric push rod is closed. When the centrifugal disk rotates, the rotating plate rotates at the lower end of the connecting plate through the rotating column, thereby ensuring that the sealing plug seals the upper end of the test tube. By setting the sealing component, the upper end of the test tube can be sealed according to different heights of the test tube to avoid leakage of the solution in the test tube.

[0016] 2. The utility model sets an annular plate. When the centrifugal disc rotates, the centrifugal disc drives the folding plate to rotate through the connecting block. The rollers in the folding plate slide in the annular groove of the annular plate. At the same time, the rollers rotate in the folding plate. A plurality of rollers can support the centrifugal disc. By setting a plurality of rollers, the rotation of the centrifugal disc can be supported, so that the high-speed rotation stability of the centrifugal disc is better. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a three-dimensional diagram of the utility model;

[0018] Figure 2 This is a cutaway perspective view of the separation chassis of the utility model;

[0019] Figure 3 This is a cutaway perspective view of the fixed tube of the utility model;

[0020] In the figure: 1. separation chassis; 2. sealing assembly; 21. fixing frame; 22. electric push rod; 23. moving plate; 24. connecting plate; 25. rotating plate; 26. rotating column; 27. sealing plug; 28. buffer assembly; 281. fixing cylinder; 282. sliding plate; 283. sliding rod; 284. spring; 3. annular plate; 4. mounting cavity; 5. centrifugal disc; 6. slot; 7. clamping mechanism; 8. groove; 9. motor; 10. annular groove; 11. connecting block; 12. folding plate; 13. roller. DETAILED DESCRIPTION

[0021] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0022] Example 1

[0023] See also Figure 1-3 The utility model provides the following technical solutions: a stem cell separation and extraction mechanism, including a separation chassis 1, a mounting cavity 4 is arranged in the separation chassis 1, a groove 8 is arranged at the lower side of the mounting cavity 4, a motor 9 is arranged in the groove 8, a centrifugal disk 5 is arranged at the output end of the motor 9, a plurality of slots 6 are evenly distributed at the upper end of the centrifugal disk 5, a clamping mechanism 7 is arranged inside the plurality of slots 6, and a sealing component 2 is arranged at the upper end of the separation chassis 1.

[0024] Specifically, the sealing assembly 2 includes a fixed frame 21, an electric push rod 22, a movable plate 23, a connecting plate 24, a rotating plate 25, a rotating column 26, a sealing plug 27 and a buffer assembly 28, wherein a fixed frame 21 is arranged at the upper end of the separation chassis 1, an electric push rod 22 is arranged at the upper end of the fixed frame 21, a movable plate 23 is arranged at the output end of the electric push rod 22, a connecting plate 24 is arranged at the lower side of the movable plate 23, a rotating column 26 is arranged at the lower end of the connecting plate 24, a rotating plate 25 is arranged at the lower end of the rotating column 26, a sealing plug 27 is arranged at the position of the lower end of the rotating plate 25 corresponding to the slot 6, and a buffer assembly 28 is arranged between the connecting plate 24 and the movable plate 23.

[0025] By adopting the above technical solution, after the test tube is inserted into the slot 6, the electric push rod 22 is turned on to push the movable plate 23 and the connecting plate 24 to move downward. After the sealing plug 27 at the lower end of the rotating plate 25 is inserted into the test tube, the electric push rod 22 is closed. When the centrifugal disk 5 rotates, the rotating plate 25 rotates at the lower end of the connecting plate 24 through the rotating column 26, thereby ensuring that the sealing plug 27 seals the upper end of the test tube. By setting the sealing component 2, the upper end of the test tube can be sealed according to different heights of the test tube to avoid leakage of the solution in the test tube.

[0026] Specifically, the buffer assembly 28 includes a fixed cylinder 281, a slide plate 282, a slide rod 283 and a spring 284, wherein a plurality of fixed cylinders 281 are arranged at the upper end of the connecting plate 24, a slide plate 282 is arranged inside the fixed cylinder 281, the slide plate 282 is connected to the movable plate 23 through the slide rod 283, and a spring 284 is arranged at the lower end of the slide plate 282.

[0027] By adopting the above technical solution, when the sealing plug 27 is inserted into the test tube, the slide rod 283 pushes the slide plate 282 to slide in the fixed tube 281, and the spring 284 is compressed. The elasticity of the spring 284 is used to ensure the sealing between the sealing plug 27 and the test tube. At the same time, the buffer component 28 plays a protective role to prevent the rotating plate 25 from descending too far and causing pressure loss on the upper end of the test tube.

[0028] Specifically, the fixing frame 21 and the separation chassis 1 as well as the rotating column 26 and the rotating plate 25 are welded by pressure welding, and adjacent fixing cylinders 281 form an angle of ninety degrees.

[0029] By adopting the above technical solution, pressure welding makes the fixing frame 21 and the separation chassis 1 as well as the rotating column 26 and the rotating plate 25 more secure, and a plurality of fixing cylinders 281 can be distributed equidistantly.

[0030] Specifically, a bearing rotating seat is provided between the rotating column 26 and the connecting plate 24 , the sealing plug 27 and the rotating plate 25 are bonded by glue, and a sliding hole corresponding to the sliding rod 283 is provided at the upper end of the fixing cylinder 281 .

[0031] By adopting the above technical solution, the rotating column 26 can rotate on the connecting plate 24, the sealing plug 27 is not easy to fall off due to the glue bonding, and the sliding rod 283 can slide on the fixing tube 281.

[0032] When the present embodiment is used, when the stem cell separation and extraction mechanism is used, the device is installed in a suitable position, and the test tube is inserted into the slot 6 of the centrifugal disk 5. The clamping mechanism 7 in the slot 6 clamps and fixes the test tube, and the motor 9 is turned on to drive the centrifugal disk 5 to rotate to centrifuge the solution in the test tube. By setting the sealing component 2, after the test tube is inserted into the slot 6, the electric push rod 22 is turned on to push the moving plate 23 and the connecting plate 24 to move downward, and the sealing plug 27 at the lower end of the rotating plate 25 is inserted into the test tube and then the electric push rod 22 is closed. When the centrifugal disk 5 rotates, the rotating plate 25 is rotated through the rotating column 26. The lower end of the connecting plate 24 rotates to ensure that the sealing plug 27 seals the upper end of the test tube. By setting the sealing component 2, the upper end of the test tube can be sealed according to different heights of the test tube to avoid leakage of the solution in the test tube. By setting the buffer component 28, when the sealing plug 27 is inserted into the test tube, the slide bar 283 pushes the slide plate 282 to slide in the fixed cylinder 281, and the spring 284 is compressed. The elasticity of the spring 284 is used to ensure the sealing between the sealing plug 27 and the test tube. At the same time, the buffer component 28 plays a protective role to prevent the rotating plate 25 from descending too far and causing pressure loss on the upper end of the test tube.

[0033] Example 2

[0034] The difference between this embodiment and embodiment 1 is that: a plurality of connecting blocks 11 are arranged on the side of the centrifugal disc 5, a folding plate 12 is arranged at one end of the connecting block 11, a roller 13 is arranged in the folding plate 12, an annular plate 3 is arranged at a position corresponding to the roller 13 in the installation cavity 4, and an annular groove 10 corresponding to the roller 13 is arranged on the inner side of the annular plate 3.

[0035] By adopting the above technical solution, when the centrifugal disc 5 rotates, the centrifugal disc 5 drives the folding plate 12 to rotate through the connecting block 11, and the roller 13 in the folding plate 12 slides in the annular groove 10 of the annular plate 3. At the same time, the roller 13 rotates in the folding plate 12, and a plurality of rollers 13 can support the centrifugal disc 5. By providing a plurality of rollers 13, the rotation of the centrifugal disc 5 can be supported, so that the high-speed rotation stability of the centrifugal disc 5 is better.

[0036] Specifically, the roller 13 and the folding plate 12 are rotatably connected via an axle pin, the side of the roller 13 is in close contact with the inner wall of the annular groove 10, and the annular plate 3 and the separation chassis 1 are welded by spot welding.

[0037] By adopting the above technical solution, the roller 13 can rotate in the folding plate 12, the roller 13 rolls more stably in the annular groove 10, and the spot welding makes the annular plate 3 and the separation chassis 1 stronger.

[0038] Specifically, adjacent rollers 13 form an angle of ninety degrees, and the annular plate 3 and the separation chassis 1 are welded by spot welding.

[0039] By adopting the above technical solution, a plurality of rollers 13 can be distributed equidistantly, and spot welding makes the annular plate 3 and the separation chassis 1 more secure.

[0040] When this embodiment is used, by setting the annular plate 3, when the centrifugal disk 5 rotates, the centrifugal disk 5 drives the folding plate 12 to rotate through the connecting block 11, and the roller 13 in the folding plate 12 slides in the annular groove 10 of the annular plate 3. At the same time, the roller 13 rotates in the folding plate 12, and a plurality of rollers 13 can support the centrifugal disk 5. By setting a plurality of rollers 13, the rotation of the centrifugal disk 5 can be supported, so that the high-speed rotation stability of the centrifugal disk 5 is better.

[0041] The electric push rod 22 in the utility model is an existing disclosed technology, and the selected model is FTG50.

[0042] The structure and working principle of the separation chassis 1, installation cavity 4, centrifugal disk 5, slot 6, clamping mechanism 7, groove 8 and motor 9 in the utility model have been disclosed in a stem cell separation and extraction device disclosed in Chinese patent application No. 2020222717262. Its working principle is to insert the test tube into the slot 6 of the centrifugal disk 5, the clamping mechanism 7 in the slot 6 clamps and fixes the test tube, and turn on the motor 9 to drive the centrifugal disk 5 to rotate to centrifuge the solution in the test tube.

[0043] The working principle and use process of the utility model are as follows: when the stem cell separation and extraction mechanism is used, the device is installed in a suitable position, and a test tube is inserted into the slot 6 of the centrifugal disk 5. The clamping mechanism 7 in the slot 6 clamps and fixes the test tube, and the motor 9 is turned on to drive the centrifugal disk 5 to rotate to centrifuge the solution in the test tube. By setting the sealing component 2, after the test tube is inserted into the slot 6, the electric push rod 22 is turned on to push the moving plate 23 and the connecting plate 24 to move downward, and the sealing plug 27 at the lower end of the rotating plate 25 is inserted into the test tube and then the electric push rod 22 is closed. When the centrifugal disk 5 rotates, the rotating plate 25 rotates at the lower end of the connecting plate 24 through the rotating column 26, thereby ensuring that the sealing plug 27 seals the upper end of the test tube. By setting the sealing component 2, the upper end of the test tube can be sealed according to the test tubes of different heights to avoid the solution in the test tube. In case of leakage of liquid, by setting the buffer assembly 28, when the sealing plug 27 is inserted into the test tube, the slide bar 283 pushes the slide plate 282 to slide in the fixed cylinder 281, and the spring 284 is compressed. The elasticity of the spring 284 is used to ensure the sealing between the sealing plug 27 and the test tube. At the same time, the buffer assembly 28 plays a protective role to prevent the rotating plate 25 from descending too far and causing pressure loss on the upper end of the test tube. By setting the annular plate 3, when the centrifugal disk 5 rotates, the centrifugal disk 5 drives the folding plate 12 to rotate through the connecting block 11, and the roller 13 in the folding plate 12 slides in the annular groove 10 of the annular plate 3. At the same time, the roller 13 rotates in the folding plate 12, and a plurality of rollers 13 can support the centrifugal disk 5. By setting a plurality of rollers 13, the rotation of the centrifugal disk 5 can be supported, so that the high-speed rotation stability of the centrifugal disk 5 is better.

[0044] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A stem cell separation and extraction mechanism, comprising a separation chassis, characterized in that: It comprises a separation chassis, wherein the separation chassis is provided with an installation cavity, a groove is provided at the lower side of the installation cavity, a motor is provided in the groove, a centrifugal disc is provided at the output end of the motor, a plurality of slots are evenly distributed at the upper end of the centrifugal disc, a clamping mechanism is provided inside each of the plurality of slots, and a sealing assembly is provided at the upper end of the separation chassis; The sealing assembly includes a fixed frame, an electric push rod, a movable plate, a connecting plate, a rotating plate, a rotating column, a sealing plug and a buffer assembly, wherein a fixed frame is arranged at the upper end of the separation chassis, an electric push rod is arranged at the upper end of the fixed frame, a movable plate is arranged at the output end of the electric push rod, a connecting plate is arranged at the lower side of the movable plate, a rotating column is arranged at the lower end of the connecting plate, a rotating plate is arranged at the lower end of the rotating column, a sealing plug is arranged at the position of the lower end of the rotating plate corresponding to the slot, and a buffer assembly is arranged between the connecting plate and the movable plate.

2. A stem cell separation and extraction mechanism according to claim 1, characterized in that: The buffer assembly includes a fixed cylinder, a slide plate, a slide rod and a spring, wherein a plurality of fixed cylinders are arranged on the upper end of the connecting plate, a slide plate is arranged inside the fixed cylinder, the slide plate is connected to the movable plate by a slide rod, and a spring is arranged at the lower end of the slide plate.

3. A stem cell separation and extraction mechanism according to claim 2, characterized in that: The fixing frame and the separation chassis as well as the rotating column and the rotating plate are all welded by pressure welding, and adjacent fixing cylinders form a ninety-degree angle.

4. A stem cell separation and extraction mechanism according to claim 2, characterized in that: A bearing rotating seat is arranged between the rotating column and the connecting plate, the sealing plug and the rotating plate are bonded by glue, and a sliding hole corresponding to the sliding rod is arranged at the upper end of the fixing cylinder.

5. The stem cell separation and extraction mechanism according to claim 1, characterized in that: The centrifugal disc is provided with a plurality of connecting blocks on the side, a folding plate is provided at one end of the connecting block, a roller is provided in the folding plate, an annular plate is provided at a position corresponding to the roller in the installation cavity, and an annular groove corresponding to the roller is provided inside the annular plate.

6. A stem cell separation and extraction mechanism according to claim 5, characterized in that: The rotating roller and the folding plate are rotatably connected via an axle pin, the side edge of the rotating roller is in close contact with the inner wall of the annular groove, and the annular plate and the separation chassis are spot welded.

7. The stem cell separation and extraction mechanism according to claim 5, characterized in that: The adjacent rollers are at an angle of 90 degrees, and the annular plate and the separation chassis are spot welded.

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