Multi-photon microscope capable of automatically positioning and clamping

Abstract

The utility model discloses a multi-photon microscope capable of automatically positioning and clamping, which comprises a microscope base body, a light source generator arranged in the microscope base body, a microscope arm mounted on the microscope base body, a lens cone arranged at one end of the microscope arm, an eyepiece arranged at the top of the lens cone, and an objective lens mounted at the bottom of the lens cone. The objective table is arranged below the objective lens and installed on the adjusting mechanism, the adjusting mechanism is arranged on one side of the lens arm, the coarse focusing screw is installed on the lens arm and drives the adjusting mechanism to move up and down, the positioning mechanism is arranged on the objective table, the detection piece is fixed through the positioning mechanism, and the light chopper is arranged at the bottom of the objective table. The device has the advantages that automatic positioning and clamping can be achieved, stable installation of the detection piece is guaranteed, manual clamping is not needed, and damage to the detection piece is avoided.

Description

Technical Field

[0001] The utility model belongs to the technical field of microscopes, and more specifically, particularly relates to an automatic positioning and clamping multi-photon microscope. Background Art

[0002] At present, optical microscopy is a common scientific tool, widely used in medicine, biology, materials science and other fields. However, traditional optical microscopes have certain limitations, especially when observing deep tissues or living samples, their resolution and light depth are limited. To overcome these problems, scientists have developed multiphoton microscopy, whose principle is based on nonlinear optical effects. Multiphoton microscopy uses high-power, ultrashort pulse lasers to achieve microscopic imaging. When the laser interacts with the sample, the energy of the photons is absorbed or scattered, forming a nonlinear effect. These nonlinear effects cause the light to generate high-energy photons inside the sample and change the propagation direction of the light. Multiphoton microscopy achieves imaging by exciting fluorescent substances in the sample. When the laser interacts with the sample, the fluorescent substance absorbs the laser energy and is in an excited state. During the deexcitation process, the fluorescent substance emits long-wavelength fluorescent photons. Multiphoton microscopy uses devices to collect and detect these fluorescent photons and generate images. Compared with traditional fluorescence microscopy, multiphoton microscopy has better deep imaging capabilities and higher spatial resolution.

[0003] During the detection process, the existing multiphoton microscope stage is manually clamped by a film clamp. Improper operation during the operation will cause damage to the detection film, affect the sample, and cannot achieve automatic positioning and clamping, which is very inconvenient. Utility Model Content

[0004] In view of the deficiencies of the prior art, the utility model provides a multi-photon microscope with automatic positioning and clamping to solve the problems raised in the above-mentioned background technology.

[0005] To achieve the above objectives, the utility model is implemented through the following technical solutions: an automatically positioned and clamped multi-photon microscope, comprising a mirror base body, a light source generator is arranged inside the mirror base body, the light source generator can emit a variety of light sources for sample detection, a switch button is arranged on the mirror base body, the switch button is electrically connected to the light source generator, a mirror arm is installed on the mirror base body, a lens barrel is arranged at one end of the mirror arm, an eyepiece is arranged on the top of the lens barrel, an objective lens is installed at the bottom of the lens barrel, a stage is arranged below the objective lens, the stage is installed on an adjustment mechanism, the adjustment mechanism is arranged on one side of the mirror arm, a coarse focusing screw is installed on the mirror arm, the adjustment mechanism is driven to move up and down by the coarse focusing screw, a positioning mechanism is arranged on the stage, the detection piece is fixed by the positioning mechanism, a shade is arranged at the bottom of the stage, and the brightness of the light source is adjusted by the shade.

[0006] As an optional solution of the utility model, a heat dissipation tube is arranged on the outside of the light source generator for continuous heat dissipation of the light source generator.

[0007] As an optional solution of the utility model, the adjustment mechanism includes a fine-tuning seat, one side of the fine-tuning seat is slidably matched with a lifting block, the top of the lifting block is connected to the stage, an adjustment spring is arranged in the fine-tuning seat, an adjustment block is arranged at the bottom of the adjustment spring, one side of the adjustment block is connected to the lifting block, a top block is arranged at the bottom of the adjustment block, one side of the top block is equipped with a fine focusing screw, the fine focusing screw is installed in the fine-tuning seat, and the height is fine-tuned by the fine focusing screw.

[0008] As an optional scheme of the utility model, the positioning mechanism includes a limit plate, which is symmetrically distributed on the loading platform, and symmetrical guide holes are arranged on the loading platform. A driving component is arranged at the bottom of the loading platform, and the limit plate is subjected to bidirectional or relative movement by the driving component. Symmetrical slots are arranged on the limit plate, and compression springs are assembled in the slots. A limit block is arranged on one side of the compression spring, and the limit block is slidably fitted in the limit hole of the limit plate.

[0009] As an optional solution of the utility model, a pull ring is further provided on one side of the limit block, and the limit block can be contracted by the pull ring.

[0010] As an optional solution of the utility model, the driving assembly includes a motor, the motor is installed at the bottom of the stage, the motor driving end is equipped with a main gear, a sub-gear is meshed with one side of the main gear, the sub-gear is fixed on a positioning ring, the positioning ring is assembled on a positioning bearing, the positioning bearing is installed at the bottom of the stage, a pair of rotating arms are installed on the top of the positioning ring through a pin shaft, the rotating arms are arc-shaped, distributed clockwise on the positioning ring, and the rotating arms are connected to the limit plate by passing through a guide hole.

[0011] The utility model provides a multi-photon microscope with automatic positioning and clamping, which has the following beneficial effects:

[0012] The height is adjusted precisely through the fine-tuning seat and the coarse focusing screw on the mirror arm, the motor is started to drive the limit plate to move, and the detection piece is placed on the stage. The motor drives the rotating arm on the positioning ring to rotate, and the rotating arm drives the limit plate to automatically position and clamp. The limit block is then retracted through the pull ring to limit the top of the detection piece and improve the positioning stability. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 It is a schematic diagram of the structure of the utility model;

[0014] Figure 2It is a partial enlarged view of the utility model;

[0015] Figure 3 It is a structural schematic diagram of another perspective of the utility model;

[0016] Figure 4 It is a front view of the utility model;

[0017] Figure 5 It is a cross-sectional view of the utility model.

[0018] In the figure: 1. lens base body; 101. switch button; 2. light source generator; 201. heat sink; 3. lens arm; 301. coarse focusing screw; 4. lens barrel; 401. eyepiece; 402. objective lens; 5. fine adjustment seat; 501. fine focusing screw; 502. adjustment spring; 6. stage; 601. guide hole; 7. detection sheet; 8. limit plate; 801. compression spring; 9. limit block; 901. pull ring; 10. motor; 11. main gear; 12. positioning ring; 121. sub gear; 122. rotating arm; 13. positioning bearing; 14. sunshade. DETAILED DESCRIPTION

[0019] The following is a further detailed description of the implementation of the present utility model in conjunction with the accompanying drawings and examples. The following examples are used to illustrate the present utility model, but cannot be used to limit the scope of the present utility model.

[0020] In the description of the present invention, unless otherwise specified, "multiple" means two or more; the terms "upper", "lower", "left", "right", "inner", "outer", "front end", "rear end", "head", "tail" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, which is only for the convenience of describing the present invention and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operate in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", "third" and the like are only used for descriptive purposes and cannot be understood as indicating or implying relative importance.

[0021] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "connected" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0022] See also Figures 1 to 5The utility model provides a technical solution: an automatic positioning and clamping multi-photon microscope, comprising a mirror base body 1, a light source generator 2 is arranged inside the mirror base body 1, the light source generator 2 can emit a variety of light sources for sample detection, a heat dissipation tube 201 is arranged on the outside of the light source generator 2, which is used for continuous heat dissipation of the light source generator 2 to improve the continuity of operation, a switch button 101 is arranged on the mirror base body 1, the switch button 101 is electrically connected to the light source generator 2, and controls the switch of the light source generator 2, a mirror arm 3 is installed on the mirror base body 1, a lens barrel 4 is arranged at one end of the mirror arm 3, an eyepiece 401 is arranged on the top of the lens barrel 4, an objective lens 402 is installed at the bottom of the lens barrel 4, a stage 6 is arranged below the objective lens 402, and the stage 6 is installed on the adjustment mechanism, the adjustment mechanism is arranged on one side of the mirror arm 3, a coarse focusing screw 301 is installed on the mirror arm 3, and the adjustment mechanism is driven to move up and down by the coarse focusing screw 301.

[0023] The adjustment mechanism includes a fine-tuning seat 5, one side of the fine-tuning seat 5 is slidably matched with a lifting block, the top of the lifting block is connected to the stage 6, an adjustment spring 502 is arranged in the fine-tuning seat 5, an adjustment block is arranged at the bottom of the adjustment spring 502, one side of the adjustment block is connected to the lifting block, a top block is arranged at the bottom of the adjustment block, one side of the top block is equipped with a fine focusing screw 501, the fine focusing screw 501 is installed in the fine-tuning seat 5, the height is fine-tuned by the fine focusing screw 501, a positioning mechanism is arranged on the stage 6, the detection piece 7 is fixed by the positioning mechanism, a light shield 14 is arranged at the bottom of the stage 6, the light source is adjusted by the light shield 14, it is convenient for detection and observation, and the positioning mechanism is provided on the stage 6. The positioning mechanism includes a limit plate 8, which is symmetrically distributed on the sample carrier 6. Symmetrical guide holes 601 are arranged on the sample carrier 6. A driving component is arranged at the bottom of the sample carrier 6, and the limit plate 8 is subjected to bidirectional or relative movement by the driving component. Symmetrical slots are arranged on the limit plate 8, and compression springs 801 are assembled in the slots. A limit block 9 is arranged on one side of the compression spring 801. The limit block 9 slides in the limit hole of the limit plate 8. A pull ring 901 is also arranged on one side of the limit block 9. The limit block 9 can be retracted by the pull ring 901 to facilitate the insertion of the detection sheet 7, and then return to limit the upper part of the detection sheet 7, thereby further improving the positioning stability.

[0024] The driving assembly includes a motor 10, which is installed at the bottom of the worktable 6. A main gear 11 is installed at the driving end of the motor 10. A sub-gear 121 is meshed on one side of the main gear 11. The sub-gear 121 is fixed on a positioning ring 12. The positioning ring 12 is assembled on a positioning bearing 13. The positioning bearing 13 is installed at the bottom of the worktable 6. A pair of rotating arms 122 are installed on the top of the positioning ring 12 through a pin shaft. The rotating arms 122 are arc-shaped and distributed clockwise on the positioning ring 12. The rotating arms 122 are connected to the limit plate 8 by passing through the guide hole 601. Starting the motor 10 drives the positioning ring 12 to rotate, thereby driving the rotating arm 122 to rotate clockwise. The limit plate 8 will perform relative or reverse motion to achieve automatic clamping and positioning.

[0025] The specific usage and function of this embodiment are as follows: when starting the detection, turn on the light source generator 2, adjust the light source through the shade 14, accurately adjust the height through the fine-tuning seat 5 and the coarse focusing screw 301 on the mirror arm 3, start the motor 10, drive the limit plate 8 to move, place the detection piece 7 on the stage 6, start the motor 10 for automatic positioning and clamping, and then retract the limit block 9 through the pull ring 901 to limit the top of the detection piece 7 to improve the positioning stability.

[0026] The device embodiments described above are merely illustrative, wherein the units described as separate components may or may not be physically separated, and the components displayed as units may or may not be physical units, that is, they may be located in one place, or they may be distributed on multiple network units. Some or all of the modules may be selected according to actual needs to achieve the purpose of the scheme of this embodiment. Ordinary technicians in this field can understand and implement it without paying creative labor.

[0027] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the utility model, rather than to limit it. Although the utility model has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the various embodiments of the utility model.

Claims

1. A multi-photon microscope with automatic positioning and clamping, characterized in that: The invention comprises a mirror base body (1), wherein a light source generator (2) is arranged inside the mirror base body (1), wherein the light source generator (2) can emit a variety of light sources for detecting samples, wherein a switch button (101) is arranged on the mirror base body (1), wherein the switch button (101) is electrically connected to the light source generator (2), wherein a mirror arm (3) is mounted on the mirror base body (1), wherein a mirror barrel (4) is arranged at one end of the mirror arm (3), wherein an eyepiece (401) is arranged at the top of the mirror barrel (4), and an objective lens (401) is mounted at the bottom of the mirror barrel (4). (402), a stage (6) is arranged below the objective lens (402), the stage (6) is mounted on an adjustment mechanism, the adjustment mechanism is arranged on one side of the mirror arm (3), a coarse focusing screw (301) is mounted on the mirror arm (3), the adjustment mechanism is driven to move up and down by the coarse focusing screw (301), a positioning mechanism is arranged on the stage (6), the detection sheet (7) is fixed by the positioning mechanism, and a light shield (14) is arranged at the bottom of the stage (6), the brightness of the light source is adjusted by the light shield (14).

2. The multi-photon microscope with automatic positioning and clamping according to claim 1, characterized in that: A heat dissipation cylinder (201) is arranged outside the light source generator (2) for continuous heat dissipation of the light source generator (2).

3. The multi-photon microscope with automatic positioning and clamping according to claim 1, characterized in that: The adjustment mechanism comprises a fine-tuning seat (5), one side of the fine-tuning seat (5) is slidably matched with a lifting block, the top of the lifting block is connected to the stage (6), an adjustment spring (502) is arranged in the fine-tuning seat (5), the bottom of the adjustment spring (502) is arranged with an adjustment block, one side of the adjustment block is connected to the lifting block, the bottom of the adjustment block is arranged with a top block, one side of the top block is equipped with a fine focusing screw (501), the fine focusing screw (501) is installed in the fine-tuning seat (5), and the height is finely adjusted by the fine focusing screw (501).

4. The multi-photon microscope with automatic positioning and clamping according to claim 1, characterized in that: The positioning mechanism comprises a limit plate (8), wherein the limit plate (8) is symmetrically distributed on the loading platform (6), and the loading platform (6) is provided with symmetrical guide holes (601). A driving component is provided at the bottom of the loading platform (6), and the limit plate (8) is subjected to bidirectional or relative movement by the driving component. The limit plate (8) is provided with symmetrical slots, and a compression spring (801) is installed in the slot. A limit block (9) is provided on one side of the compression spring (801), and the limit block (9) is slidably fitted in the limit hole of the limit plate (8).

5. The multi-photon microscope with automatic positioning and clamping according to claim 4, characterized in that: A pull ring (901) is also provided on one side of the limit block (9), and the limit block (9) can be retracted via the pull ring (901).

6. The multi-photon microscope with automatic positioning and clamping according to claim 4, characterized in that: The driving assembly comprises a motor (10), wherein the motor (10) is mounted at the bottom of the stage (6), a main gear (11) is mounted at the driving end of the motor (10), a sub-gear (121) is meshed with one side of the main gear (11), the sub-gear (121) is fixed on a positioning ring (12), the positioning ring (12) is mounted on a positioning bearing (13), the positioning bearing (13) is mounted at the bottom of the stage (6), a pair of rotating arms (122) are mounted on the top of the positioning ring (12) via a pin, the rotating arms (122) are arc-shaped, and are distributed on the positioning ring (12) in a clockwise direction, and the rotating arms (122) are connected to the limit plate (8) by passing through a guide hole (601).

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