Cell culture toxicology detector

Abstract

The utility model discloses a cell culture toxicology detector which comprises a box body, a cell culture chamber and a toxicology detection chamber are arranged in the box body, the cell culture chamber is located at the top end in the box body, the cell culture chamber is located at the bottom end in the box body, and a dosing device and an observation device are arranged in the toxicology detection chamber. A temperature stabilizing device is arranged on one side of the box body; the cell culture device integrates the functions of cell culture, dosing and drug toxicology detection, efficiency and accuracy are effectively improved, the purpose of cell culture to drug toxicology detection is achieved, and disease treatment and drug research and development are further facilitated.

Description

Technical Field

[0001] The utility model relates to the technical field of medicine, in particular to a cell culture toxicology detector. Background Art

[0002] Transdermal drugs are prone to irritating the skin and even causing tissue necrosis, so their safety assessment is very important. However, traditional transdermal toxicological evaluation methods have long detection cycles and low controllability of the experimental process; and the artificial skin manufacturing process widely used in drug screening has high costs, low reproducibility, and large batch differences. Therefore, the current skin model cannot perform complex evaluations, such as evaluating the sensitization and toxicity of internal organs; the skin chip with microfluidic chip technology, combined with the technical detection device of liver neurons and skin system, can realize rapid and accurate toxicological evaluation and screening of drugs in the skin and liver, so as to reduce the use of animal experiments and the risks of human experiments, and improve the accuracy of toxicological evaluation.

[0003] At present, cell culture and testing on the market are carried out in steps. For example, operators need to extract cells from the culture medium. During this process, whether the cells are in contact with the outside world or accidentally damaged, there are certain risks, which affects the efficiency of the experiment. Therefore, a cell culture toxicology detector is proposed to solve the above problems. Utility Model Content

[0004] In order to make up for the above deficiencies, the utility model provides a cell culture toxicology detector, aiming to improve the problems in the comparative documents.

[0005] In order to achieve the above-mentioned purpose, the utility model adopts the following technical scheme: a cell culture toxicology detector, comprising a box body, wherein the box body comprises a cell culture chamber and a toxicology detection chamber, wherein the cell culture chamber is located at the top of the box body, and the cell culture chamber is located at the bottom of the box body, wherein a dosing device and an observation device are arranged inside the toxicology detection chamber, and a temperature stabilizing device is arranged on one side of the box body;

[0006] As a further description of the above technical solution: an electric guide rail is provided at the inner bottom end of the cell culture chamber, an electric slider is provided inside the electric guide rail, a bracket is provided on the surface of the electric slider, a cell culture platform is provided on the top surface of the bracket, and a microfluidic chip culture plate is provided on the surface of the cell culture platform;

[0007] As a further description of the above technical solution: the dosing device includes a chassis, a liquid storage box is provided inside the chassis, a first solenoid valve is provided at the bottom end of the liquid storage box, a dripping tube is provided at the bottom end of the first solenoid valve, an air pump is provided at one side of the chassis, an air supply pipe is provided at the bottom end of the air pump, and the air supply pipe is connected to the dripping tube;

[0008] As a further description of the above technical solution: the temperature stabilizing device includes an air outlet, the air outlet is located on one side of the cell culture chamber, an exhaust pump is provided on one side of the air outlet, an air pipe is provided on one side of the exhaust pump, a water cooling box is sleeved on one surface of the air pipe, and the air pipe runs through the water cooling box, a heating box is provided at the bottom end of one side of the air pipe, a reflux pipe is provided at the bottom end of the heating box, and the reflux pipe is connected to the inside of the cell culture chamber.

[0009] As a further description of the above technical solution: a second solenoid valve is provided on the surface of the air supply pipe.

[0010] As a further description of the above technical solution: the observation device is a fluorescence microscope imager, and the electrical signal of the fluorescence microscope imager is connected to the electric terminal.

[0011] As a further description of the above technical solution: a culture layer is provided on the surface of the microfluidic chip culture plate, and the culture layer is divided into four groups, and a loading hole and a waste liquid hole are provided on the surface of the culture layer, and the loading hole and the waste liquid hole are connected to form a loop, and one side of the culture layer is a culture hole.

[0012] As a further description of the above technical solution: the cell culture chamber and the toxicology detection chamber are inside the box and are in the shape of drawers that can be pulled out from one side of the box.

[0013] As a further description of the above technical solution: a liquid filling hole is provided on one side of the liquid storage box.

[0014] Compared with the prior art, the beneficial effects of the utility model are as follows:

[0015] 1. The utility model integrates the functions of cell culture, drug addition, and drug toxicology detection, effectively improving efficiency and accuracy, achieving the purpose from cell culture to drug toxicology detection, and further facilitating disease treatment and drug development.

[0016] 2. Under the action of the exhaust pump, the utility model first cools the gas in the cell culture chamber through a water cooling box, and then heats it through a heating box. The heated gas returns to the cell culture chamber from the air inlet pipe, so the cycle achieves a constant temperature effect and ensures the growth environment of the cells. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0018] Figure 2 It is a schematic diagram of the other side of the overall structure of the utility model;

[0019] Figure 3 It is a schematic diagram of the internal structure of the box of the utility model;

[0020] Figure 4 It is a structural schematic diagram of the A part of the utility model;

[0021] Figure 5 It is a schematic diagram of the local structure of the microfluidic chip culture plate of the utility model;

[0022] Figure 6 This is a schematic diagram of the structure of the dosing device of the utility model;

[0023] Legend:

[0024] 1. Box body; 2. Electric guide rail; 3. Electric slider; 4. Bracket; 5. Cell culture platform; 6. Microfluidic chip culture plate; 7. Chassis; 8. Liquid storage box; 9. First solenoid valve; 10. Dropping tube; 11. Air pump; 12. Air supply pipe; 13. Air outlet; 14. Exhaust pump; 15. Air pipe; 16. Water cooling box; 17. Heating box; 18. Reflux pipe; 19. Liquid adding hole; 20. Second solenoid valve; 101. Cell culture room; 102. Toxicology test room; 103. Dosing device; 601. Culture layer; 602. Sample loading hole; 603. Waste liquid hole; 604. Culture hole. DETAILED DESCRIPTION

[0025] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0026] Reference Figure 1-6 The utility model provides an embodiment: a cell culture toxicology detector, comprising a housing 1, wherein the housing 1 includes a cell culture chamber 101 and a toxicology detection chamber 102, wherein the cell culture chamber 102 is located at the top of the housing 1, and the cell culture chamber 101 is located at the bottom of the housing 1, wherein a dosing device 103 and an observation device 104 are provided inside the toxicology detection chamber 102, and a temperature stabilization device 105 is provided on one side of the housing 1;

[0027] Reference Figure 1-6 The bottom of the cell culture chamber 101 is provided with an electric guide rail 2, the electric guide rail 2 is provided with an electric slider 3, the surface of the electric slider 3 is provided with a bracket 4, the top surface of the bracket 3 is provided with a cell culture platform 5, and the surface of the cell culture platform 5 is provided with a microfluidic chip culture plate 6;

[0028] Obtain complete experimental data through microfluidic chip culture plates designed for biological characteristics. Various types of chip culture plates can meet the requirements of different species and different experimental purposes, providing precise cell growth and experimental operation microenvironment

[0029] Reference Figure 1-6 The dosing device 103 includes a chassis 7, a liquid storage box 8 is provided inside the chassis 7, a first solenoid valve 9 is provided at the bottom end of the liquid storage box 8, a dripping tube 10 is provided at the bottom end of the first solenoid valve 9, an air pump 11 is provided on one side of the chassis 7, an air supply pipe 12 is provided at the bottom end of the air pump 11, and the air supply pipe 12 is connected to the dripping tube 10;

[0030] Reference Figure 1-6 The temperature stabilizing device 105 includes an air outlet 13, which is located on one side of the cell culture chamber 101. An exhaust pump 14 is provided on one side of the air outlet 13, and an air pipe 15 is provided on one side of the exhaust pump 14. A water cooling box 16 is sleeved on the surface of one side of the air pipe 15, and the air pipe 15 passes through the water cooling box 16. A heating box 17 is provided at the bottom end of one side of the air pipe 15, and a reflux pipe 18 is provided at the bottom end of the heating box 17, and the reflux pipe 18 is connected to the inside of the cell culture chamber 101.

[0031] Reference Figure 1-6 A second solenoid valve 20 is provided on the surface of the air supply pipe 12 .

[0032] Reference Figure 1-6 The observation device 104 is a fluorescence microscope imager, and the electrical signal of the fluorescence microscope imager is connected to the electric terminal.

[0033] Reference Figure 1-6 A culture layer 601 is provided on the surface of the microfluidic chip culture plate 6, and the culture layer 601 is divided into four groups. A loading hole 602 and a waste liquid hole 603 are provided on the surface of the culture layer 601. The loading hole 602 and the waste liquid hole 603 are connected to form a loop, and one side of the culture layer 601 is a culture hole 604.

[0034] Through the loading hole 602: add different reagents and culture medium, the waste liquid hole 603: discharge the metabolic waste liquid generated in the cell culture, and the culture hole 604: observe and culture cells;; Among them, the loading hole 602, according to the requirements of the experiment, can add different reagents and culture medium, the metabolic waste liquid generated by the observation and culture of cells in the culture is discharged from the right side and enters the waste liquid hole. There are four cell culture layers 101 on a chip board, each chamber is connected to six loading holes and two waste liquid holes, forming an independent circuit. By setting different experimental conditions, four different experimental processing designs and data collection can be formed at the same time

[0035] Reference Figure 1-6 The cell culture chamber 101 and the toxicology detection chamber 102 are inside the box body 1 and are in the shape of drawers that can be pulled out from one side of the box body 1 .

[0036] Reference Figure 1-6 A liquid adding hole 19 is provided on one side of the liquid storage box 8.

[0037] Working principle: Cell culture chamber 101: The cell culture chamber 101 can be withdrawn from one side of the housing 1 as a whole, so that the microfluidic chip culture plate 6 can be taken out to achieve the effect of automatic cell culture; after the cultured cells are placed on the cell culture platform 5, the electric slider 3 is controlled to move on the electric track 2, so that the microfluidic chip culture plate 6 can be moved to the bottom of the dosing device 103, and RPMI1640 culture solution, calf serum, penicillin and streptomycin can be added to the liquid storage box 8 through the liquid adding hole 19, and the liquid in the liquid storage box 8 is dripped onto the culture hole 604 on the fluidic chip culture plate 6 through the drip tube 10 by controlling the opening of the first solenoid valve 9, and then the second solenoid 20 and the air pump 11 are started. , the liquid in the dropper 10 can be sucked out and added quantitatively; when detection is required, it is necessary to control the electric slider 3 to move on the electric track 2, so that the microfluidic chip culture plate 6 is located below the toxicology test room 102, and the microfluidic chip culture plate 6 is detected by a fluorescent microscope imager, and the data is transmitted to the computer terminal (this is the prior art and no further elaboration is required); during the long-term culture process, the gas in the cell culture chamber 101 is pumped into the air pipe 15 through the air outlet 13 by the exhaust pump 14, and the air pipe 15 is first cooled by water through the water cooling box 16, and then the gas is sent to the heating box 17 for heating and heating, and finally the gas is sent back by the reflux pipe 18, so as to achieve a constant temperature effect.

[0038] Finally, it should be noted that the above description is only a preferred embodiment of the utility model and is not intended to limit the utility model. Although the utility model is described in detail with reference to the above embodiments, those skilled in the art can still modify the technical solutions recorded in the above embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the utility model shall be included in the protection scope of the utility model.

Claims

1. A cell culture toxicology detector, comprising a housing (1), characterized in that: The housing (1) comprises a cell culture chamber (101) and a toxicology detection chamber (102), wherein the cell culture chamber (101) is located at the top of the housing (1), and the cell culture chamber (102) is located at the bottom of the housing (1). A dosing device (103) and an observation device (104) are provided inside the toxicology detection chamber (102), and a temperature stabilization device (105) is provided on one side of the housing (1); The bottom of the cell culture chamber (101) is provided with an electric guide rail (2), an electric slider (3) is provided inside the electric guide rail (2), a support (4) is provided on the surface of the electric slider (3), a cell culture platform (5) is provided on the top surface of the support (4), and a microfluidic chip culture plate (6) is provided on the surface of the cell culture platform (5); The dosing device (103) comprises a case (7), a liquid storage box (8) is provided inside the case (7), a first solenoid valve (9) is provided at the bottom end of the liquid storage box (8), a dripping tube (10) is provided at the bottom end of the first solenoid valve (9), an air pump (11) is provided on one side of the case (7), an air supply pipe (12) is provided at the bottom end of the air pump (11), and the air supply pipe (12) is connected to the dripping tube (10); The temperature stabilizing device (105) comprises an air outlet (13), the air outlet (13) being located at one side of the cell culture chamber (101), an exhaust pump (14) being provided at one side of the air outlet (13), an air pipe (15) being provided at one side of the exhaust pump (14), a water cooling box (16) being sleeved on one side surface of the air pipe (15), and the air pipe (15) passing through the water cooling box (16), a heating box (17) being provided at the bottom end of one side of the air pipe (15), a return pipe (18) being provided at the bottom end of the heating box (17), and the return pipe (18) being connected to the inside of the cell culture chamber (101).

2. A cell culture toxicology detector according to claim 1, characterized in that: A second solenoid valve (20) is provided on the surface of the air supply pipe (12).

3. A cell culture toxicology detector according to claim 1, characterized in that: The observation device (104) is a fluorescence microscope imager, and the electrical signal of the fluorescence microscope imager is connected to an electric terminal.

4. A cell culture toxicology detector according to claim 1, characterized in that: The surface of the microfluidic chip culture plate (6) is provided with a culture layer (601), and the culture layer (601) is divided into four groups. The surface of the culture layer (601) is provided with a loading hole (602) and a waste liquid hole (603), and the loading hole (602) and the waste liquid hole (603) are connected to form a loop, and one side of the culture layer (601) is a culture hole (604).

5. A cell culture toxicology detector according to claim 1, characterized in that: The cell culture chamber (101) and the toxicology detection chamber (102) are located inside the box (1) and are in the shape of drawers that can be pulled out from one side of the box (1).

6. A cell culture toxicology detector according to claim 1, characterized in that: A liquid adding hole (19) is provided on one side of the liquid storage box (8).

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