Portable DNA extraction device

Abstract

The utility model discloses a portable DNA (deoxyribonucleic acid) extraction device which comprises a box body, a box cover is arranged at the top of the box body, a handle is fixedly connected to the top of the box cover, a placing seat is arranged in the box body, sliding seats are fixedly connected to the left end and the right end of the placing seat, and the sliding seats are in sliding connection with the box body. A sliding seat is fixedly connected to the bottom of the box cover, a ball is movably sleeved with the sliding seat, the ball is slidably connected with the box body, a fixing rod is fixedly connected to the bottom of the box cover, a fixing plate is fixedly connected to the bottom of the fixing rod, a positioning mechanism is arranged on the placing seat, and a taking and placing mechanism is arranged on the placing seat. The test tube can be clamped and positioned through the cooperation of the clamping blocks and the second springs, and the test tube can be pressed and positioned through the cooperation of the pressing blocks and the first springs, so that the test tube can be positioned in multiple directions at the same time, the stability is better, and the test tube is safer to carry and use.

Description

Technical Field

[0001] The utility model relates to the technical field of extraction devices, in particular to a portable DNA extraction device. Background Art

[0002] Deoxyribonucleic acid is one of the four biological macromolecules contained in biological cells. It is a macromolecular polymer composed of deoxynucleotides. In the DNA molecular structure, two polydeoxynucleotide chains are coiled around a common central axis to form a double helix structure. DNA is usually isolated from blood, skin, saliva, hair and other tissues and body fluids to identify criminals or criminal behavior. In addition, modern biology and biochemistry use DNA extensively. DNA extraction kits are constructed based on the benzyl chloride method and are kits that can extract genomic DNA from samples.

[0003] The utility model patent with the patent authorization announcement number CN213800880U discloses a DNA extraction kit, including a placement box, a sealing cover, a placement frame and a handle, wherein the port of the upper port of the placement box is sealed and connected with a sealing cover through two sets of pages, and two sets of U-shaped structure mounting parts are bolted to the upper surface of the sealing cover, and a handle is movably connected between the two sets of mounting parts; the placement box is provided with a rectangular placement frame and a parallel bidirectional lead screw from top to bottom, and multiple groups of reagent tube placement slots are evenly distributed on the top of the placement frame, and each group of placement slots is provided with two groups of arc blocks and two groups of clamping mechanisms, and the two groups of vertical outer walls of the placement frame are fixedly welded with sliding blocks, and the placement frame is slidably connected with the sliding grooves opened on the two groups of vertical inner walls of the placement box through the two groups of sliding blocks; the left end of the bidirectional lead screw is rotatably connected with a group of vertical inner walls of the placement box through a bearing. The DNA extraction kit has a simple structure and strong practicality.

[0004] In the above-mentioned prior art, during the use of the reagent kit, the test tube is limited in the horizontal direction by a spring clamping method, and the positioning effect is insufficient. The shaking of the device when it is carried will also cause the test tube to shake in the vertical direction, which is not stable enough. In addition, the bidirectional lead screw lacks corresponding limiting means, and may rotate due to shaking when carried, which will affect the stability of the test tube. Therefore, improvements are needed. Utility Model Content

[0005] The utility model aims to provide a portable DNA extraction device, which solves the problem of insufficient test tube positioning effect and the problem of insufficient stability of the test tube when being carried.

[0006] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a portable DNA extraction device, comprising a box body, a box cover is arranged on the top of the box body, a handle is fixedly connected to the top of the box cover, a placement seat is arranged inside the box body, a sliding seat is fixedly connected to the left and right ends of the placement seat, the sliding seat is slidably connected to the box body, a ball is movably sleeved inside the sliding seat, the ball is slidably connected to the box body, a fixing rod is fixedly connected to the bottom of the box cover, a fixing plate is fixedly connected to the bottom of the fixing rod, a positioning mechanism is arranged on the placement seat, and a pick-up and placement mechanism is arranged on the placement seat.

[0007] Preferably, a slide groove is provided inside the box body, and a ball is slidably connected inside the slide groove. The slide groove is designed so that the ball can slide along the box body.

[0008] Preferably, the positioning mechanism includes a pressing block, a plurality of pressing blocks are slidably sleeved inside the fixed plate, a sponge block is fixedly connected to the bottom of the pressing block, a slider is fixedly connected to the left and right ends of the pressing block, the slider is slidably connected to the fixed plate, a first spring is fixedly connected to the top of the slider, the first spring is fixedly connected to the fixed plate, a sliding rod is slidably sleeved inside the placement seat, a second spring is arranged on the outside of the sliding rod, a clamping block is fixedly connected to the outside of the sliding rod, the clamping block is slidably connected to the placement seat, and a plurality of evenly distributed sponge pads are fixedly connected inside the placement seat. By designing a positioning mechanism, the test tube can be positioned.

[0009] Preferably, one end of the second spring is fixedly connected to the clamping block, and the other end of the second spring is fixedly connected to the placement seat. The second spring is designed so that the force of the second spring can act on the clamping block.

[0010] Preferably, the pick-and-place mechanism comprises a screw sleeve, the bottom of the placement seat is fixedly connected with a screw sleeve, the interior of the screw sleeve is connected with a screw rod by threading, the screw rod is rotatably connected to the box body by a bearing, the outer side of the screw rod is fixedly sleeved with a first bevel gear, the right end of the first bevel gear is meshed with a second bevel gear, the right end of the second bevel gear is fixedly connected with a rotating shaft, the rotating shaft is rotatably connected to the box body by a bearing, the right end of the box body is provided with a slot, the interior of the slot is slidably connected with a plug rod, the outer side of the plug rod is fixedly connected with a connecting ring, the interior of the connecting ring is slidably sleeved with a fixing ring, the fixing ring is fixedly connected to the rotating shaft, the inner side of the connecting ring is fixedly connected with two symmetrically distributed guide blocks, the guide blocks are slidably connected to the fixing ring, the right end of the guide block is fixedly connected with a third spring, and the third spring is fixedly connected to the fixing ring. By designing the pick-and-place mechanism, it is convenient to take and place the test tube.

[0011] Preferably, there are multiple slots, which are evenly distributed in a ring shape on the box body. The slots are designed so that the insertion rod can be inserted into the slots at different positions.

[0012] Compared with the prior art, the beneficial effects of the utility model are as follows:

[0013] 1. The utility model can clamp and position the test tube by designing the cooperation between the clamping block and the second spring, and can press the test tube downward by using the pressure block in cooperation with the first spring, so that the test tube can be positioned in multiple directions at the same time, which has better stability and is safer when carried and used.

[0014] 2. The utility model designs a threaded connection between the screw and the screw sleeve. The screw sleeve can be threadedly moved by rotating the rotating shaft, thereby realizing the lifting and lowering of the placement seat, which is convenient for taking and placing the test tube. At the same time, the rotating shaft can be limited by plugging the insertion rod into the slot, which can prevent the shaking during carrying from causing the screw to rotate and cause the test tube to move inside the box. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 It is a three-dimensional diagram of the overall structure of the utility model;

[0016] Figure 2 This utility model Figure 1 A three-dimensional cross-sectional view of the local structure;

[0017] Figure 3 This utility model Figure 2 A magnified image of point A;

[0018] Figure 4 This utility model Figure 2 The enlarged view of point B;

[0019] Figure 5 This utility model Figure 2 Enlarged view of point C.

[0020] In the figure: 1, box body; 2, box cover; 3, handle; 4, placement seat; 5, slide seat; 6, ball bearing; 7, slide groove; 8, positioning mechanism; 9, pick-and-place mechanism; 10, fixing rod; 11, fixing plate; 81, pressure block; 82, sponge block; 83, slider; 84, first spring; 85, slide rod; 86, second spring; 87, clamping block; 88, sponge pad; 91, screw sleeve; 92, screw rod; 93, first bevel gear; 94, second bevel gear; 95, rotating shaft; 96, slot; 97, plug rod; 98, connecting ring; 99, fixing ring; 991, guide block; 992, third spring. DETAILED DESCRIPTION

[0021] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0022] See also Figure 1 , Figure 2 A portable DNA extraction device comprises a box body 1, a box cover 2 is arranged on the top of the box body 1, a handle 3 is fixedly connected to the top of the box cover 2, a placement seat 4 is arranged inside the box body 1, a slide seat 5 is fixedly connected to the left and right ends of the placement seat 4, the slide seat 5 is slidably connected to the box body 1, a ball 6 is movably sleeved inside the slide seat 5, the ball 6 is slidably connected to the box body 1, a slide groove 7 is opened inside the box body 1, and the ball 6 is slidably connected inside the slide groove 7. By designing the slide groove 7, the ball 6 can slide along the box body 1, a fixing rod 10 is fixedly connected to the bottom of the box cover 2, and a fixing plate 11 is fixedly connected to the bottom of the fixing rod 10, a positioning mechanism 8 is arranged on the placement seat 4, and a pick-up and placement mechanism 9 is arranged on the placement seat 4.

[0023] See also Figure 1 , Figure 2 , Figure 3 , Figure 4 The positioning mechanism 8 includes a pressing block 81, a plurality of pressing blocks 81 are slidably sleeved inside the fixed plate 11, a sponge block 82 is fixedly connected to the bottom of the pressing block 81, a slider 83 is fixedly connected to the left and right ends of the pressing block 81, the slider 83 is slidably connected to the fixed plate 11, a first spring 84 is fixedly connected to the top of the slider 83, the first spring 84 is fixedly connected to the fixed plate 11, a sliding rod 85 is slidably sleeved inside the placement seat 4, a second spring 86 is arranged on the outside of the sliding rod 85, one end of the second spring 86 is fixedly connected to the clamping block 87, and the other end of the second spring 86 is fixedly connected to the placement seat 4. By designing the second spring 86, the force of the second spring 86 can act on the clamping block 87, a clamping block 87 is fixedly connected to the outside of the sliding rod 85, the clamping block 87 is slidably connected to the placement seat 4, and a plurality of evenly distributed sponge pads 88 are fixedly connected to the inside of the placement seat 4. By designing the positioning mechanism 8, the test tube can be positioned.

[0024] See also Figure 1 , Figure 2 , Figure 5The pick-and-place mechanism 9 includes a screw sleeve 91, a screw sleeve 91 is fixedly connected to the bottom of the placement seat 4, a screw sleeve 92 is threadedly connected to the inside of the screw sleeve 91, the screw 92 is rotatably connected to the box body 1 through a bearing, a first bevel gear 93 is fixedly sleeved on the outside of the screw 92, a second bevel gear 94 is meshed at the right end of the first bevel gear 93, a rotating shaft 95 is fixedly connected to the right end of the second bevel gear 94, the rotating shaft 95 is rotatably connected to the box body 1 through a bearing, a slot 96 is opened at the right end of the box body 1, and the number of the slots 96 is multiple, and the multiple slots 96 are annular and evenly distributed on the box body 1, and the slots 96 are designed to be uniformly distributed. The slot 96 allows the insertion rod 97 to be inserted into the slot 96 at different positions. The slot 96 is slidably connected to the insertion rod 97, and the outer side of the insertion rod 97 is fixedly connected to the connection ring 98. The inner side of the connection ring 98 is slidably sleeved with a fixing ring 99, and the fixing ring 99 is fixedly connected to the rotating shaft 95. The inner side of the connection ring 98 is fixedly connected to two symmetrically distributed guide blocks 991, and the guide blocks 991 are slidably connected to the fixing ring 99. The right end of the guide block 991 is fixedly connected to a third spring 992, and the third spring 992 is fixedly connected to the fixing ring 99. The design of the pick-up and placement mechanism 9 facilitates the taking and placement of the test tube.

[0025] The specific implementation process of the utility model is as follows: during use, when it is necessary to extract DNA, first collect a sufficient amount from the required biological sample, such as blood, saliva, tissue or cells, and then break the cells in the sample to release the DNA, which can be achieved by mechanical methods (such as oscillators or ultrasound), chemical methods (such as protease digestion) or physical methods (such as freeze-thaw). The collected samples are pretreated, such as centrifugation, washing or cutting, to remove possible contaminants, and the required reagents in the kit are added to the sample, and then the sample and the added reagents are fully mixed, and the appropriate incubation time and temperature are performed according to the instructions of the kit. After mixing, the DNA is separated, and the DNA can be extracted after washing and purification. When it is necessary to position the test tube, the test tube is first vertically inserted into the placement seat 4. The contact between the bottom of the test tube and the clamping block 87 will push the clamping block 87 to move horizontally, and finally the test tube will contact the sponge pad 88, so that the clamping block 87 drives the sliding rod 85 to move, and the clamping block 87 squeezes the second spring 86, and the test tube can be clamped by the elastic force of the second spring 86. Then the box cover 2 is closed, and the box cover 2 will drive the fixing rod 10 and the fixing plate 11 to move downward, and the fixing plate 11 drives the pressing block 81 to move downward, and the pressing block 81 contacts the top of the test tube. As the fixing plate 11 moves downward, the fixing plate 11 squeezes the first spring 84, and the elasticity of the first spring 84 can act on the sliding block 83, and then the test tube can be pressed down by the pressing block 81 and the sponge block 82, so that the test tube can be positioned in multiple directions at the same time, with better stability and safer when carried and used. When it is necessary to take or place the test tube, the connecting ring 98 is pulled horizontally to drive the guide block 991 to move, and the guide block 991 squeezes the third spring 992. At the same time, the connecting ring 98 drives the insertion rod 97 to move horizontally and separate from the slot 96. Then the connecting ring 98 is rotated, and the connecting ring 98 drives the fixing ring 99 and the rotating shaft 95 to rotate, thereby driving the second bevel gear 94 and the first bevel gear 93 to rotate, so that the screw rod 92 can be rotated, and then the threaded movement of the screw sleeve 91 can be realized, and the lifting and lowering of the placement seat 4 can be realized, which is convenient for taking or placing the test tube. At the same time, the rotating shaft 95 can be limited by the connection between the insertion rod 97 and the slot 96, which can prevent the shaking during carrying from causing the screw rod 92 to rotate and cause the test tube to move inside the box body 1.

[0026] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A portable DNA extraction device, comprising a box body (1), characterized in that: A box cover (2) is arranged on the top of the box body (1), and a handle (3) is fixedly connected to the top of the box cover (2). A placement seat (4) is arranged inside the box body (1), and both left and right ends of the placement seat (4) are fixedly connected to slide seats (5), and the slide seat (5) is slidably connected to the box body (1). A ball (6) is movably sleeved inside the slide seat (5), and the ball (6) is slidably connected to the box body (1). A fixing rod (10) is fixedly connected to the bottom of the box cover (2), and a fixing plate (11) is fixedly connected to the bottom of the fixing rod (10). A positioning mechanism (8) is arranged on the placement seat (4), and a pick-up and placement mechanism (9) is arranged on the placement seat (4).

2. A portable DNA extraction device according to claim 1, characterized in that: A slide groove (7) is provided inside the box body (1), and a ball (6) is slidably connected inside the slide groove (7).

3. A portable DNA extraction device according to claim 1, characterized in that: The positioning mechanism (8) comprises a pressing block (81), a plurality of pressing blocks (81) are slidably sleeved inside the fixing plate (11), a sponge block (82) is fixedly connected to the bottom of the pressing block (81), a slider (83) is fixedly connected to the left and right ends of the pressing block (81), the slider (83) is slidably connected to the fixing plate (11), a first spring (84) is fixedly connected to the top of the slider (83), and the first spring (84) is fixedly connected to the fixing plate (11), a sliding rod (85) is slidably sleeved inside the placement seat (4), a second spring (86) is arranged on the outside of the sliding rod (85), a clamping block (87) is fixedly connected to the outside of the sliding rod (85), the clamping block (87) is slidably connected to the placement seat (4), and a plurality of evenly distributed sponge pads (88) are fixedly connected inside the placement seat (4).

4. A portable DNA extraction device according to claim 3, characterized in that: One end of the second spring (86) is fixedly connected to the clamping block (87), and the other end of the second spring (86) is fixedly connected to the placement seat (4).

5. A portable DNA extraction device according to claim 1, characterized in that: The pick-and-place mechanism (9) comprises a screw sleeve (91), the bottom of the placement seat (4) is fixedly connected to the screw sleeve (91), the interior of the screw sleeve (91) is connected to a screw rod (92) by means of a thread, the screw rod (92) is rotatably connected to the box body (1) by means of a bearing, a first bevel gear (93) is fixedly sleeved on the outer side of the screw rod (92), a second bevel gear (94) is meshed at the right end of the first bevel gear (93), a rotating shaft (95) is fixedly connected to the right end of the second bevel gear (94), the rotating shaft (95) is rotatably connected to the box body (1) by means of a bearing, and a plug is provided at the right end of the box body (1). A slot (96) is provided, wherein the slot (96) is slidably connected to an insertion rod (97) inside the insertion rod (97), a connecting ring (98) is fixedly connected to the outside of the insertion rod (97), a fixing ring (99) is slidably sleeved inside the connecting ring (98), the fixing ring (99) is fixedly connected to the rotating shaft (95), two symmetrically distributed guide blocks (991) are fixedly connected to the inside of the connecting ring (98), the guide blocks (991) are slidably connected to the fixing ring (99), a third spring (992) is fixedly connected to the right end of the guide block (991), and the third spring (992) is fixedly connected to the fixing ring (99).

6. A portable DNA extraction device according to claim 5, characterized in that: The number of the slots (96) is multiple, and the multiple slots (96) are annular and evenly distributed on the box body (1).

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