Transwell chamber scraper device

Abstract

A transwell chamber scraper device comprises a chamber body and a semi-permeable membrane arranged at the bottom of the chamber body, the chamber body comprises an inverted-cone-shaped barrel and an annular outer edge arranged at the upper end of the inverted-cone-shaped barrel, the transwell chamber scraper device further comprises a scraper matched with the chamber body, and the scraper comprises a scraper plate tightly attached to the bottom of the chamber body and a hand pushing part connected with the scraper plate. And the scraper can collect bubbles below the semipermeable membrane at the bottom of the small chamber body, so that the bubbles are convenient to clean.

Description

Technical Field

[0001] The utility model relates to the technical field of cell experiment instruments, in particular to a transwell chamber scraper device. Background Art

[0002] Transwell chambers are commonly used in cell experiments, such as co-culture experiments, chemotaxis experiments, cell migration, cell invasion, and drug transport experiments. When doing cell migration experiments, it is necessary to add cell suspension to the upper layer of the chamber. The semipermeable membrane in the lower layer of the chamber that is in contact with the culture medium will produce a lot of bubbles. The bubbles need to be cleaned up quickly before starting the experiment, otherwise it will affect cell migration. The current way to clean up bubbles is to use a gun tip to suck away the floating bubbles. Since the bubbles are not concentrated, it is troublesome to clean up, which is time-consuming and laborious. Summary of the invention

[0003] The technical problem to be solved by the utility model is to provide a transwell chamber scraper device, which can collect bubbles below the semi-permeable membrane at the bottom of the chamber body, so as to facilitate the cleaning of the bubbles.

[0004] In order to solve the above technical problems, the technical solution of the utility model is: a transwell chamber scraper device, including a chamber body and a semipermeable membrane arranged at the bottom of the chamber body, the chamber body including an inverted conical tube and an annular outer edge arranged at the upper end of the inverted conical tube, and also including a scraper matched with the chamber body, the scraper including a scraper closely attached to the bottom of the chamber body and a hand-push portion connected to the scraper. The principle of the utility model: in the initial state, the scraper is located on one side of the bottom of the chamber body. When it is necessary to clean the bubbles under the semipermeable membrane, the hand-push portion is used to drive the scraper so that the scraper is translated from one side of the chamber body to the other side. When the scraper passes under the semipermeable membrane, the bubbles are collected together and moved out of the bottom of the chamber body. At this time, the bubbles collected by the scraper can be conveniently sucked away by the gun head, which is quick and convenient.

[0005] As an improvement, the scraper is in an arc shape.

[0006] As an improvement, the two ends of the scraper are respectively connected to the slide rails, and the two sides of the bottom of the chamber body are provided with slide grooves, and the slide rails are slidably matched with the slide grooves.

[0007] One ends of the two slide rails are connected through a transverse connecting rod to form a sliding frame, and the top surface of the chamber body is provided with a push rod abutting against the annular outer edge, and both ends of the push rod are connected to the sliding frame through a vertical connecting rod.

[0008] As an improvement, the upper end of the vertical connecting rod is provided with a bent avoidance groove, and the annular outer edge can pass through the avoidance groove.

[0009] As an improvement, a collecting groove is provided on the inner side of the scraper.

[0010] Compared with the prior art, the utility model has the following beneficial effects:

[0011] The hand push part drives the scraper to move horizontally from one side of the chamber body to the other side. When the scraper passes under the semipermeable membrane, the bubbles are collected together and moved out of the bottom of the chamber body. At this time, the gun head can be used to conveniently suck away the bubbles collected by the scraper, which is quick and convenient. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] Figure 1 It is a three-dimensional diagram of the utility model.

[0013] Figure 2 It is a bottom view of the utility model. DETAILED DESCRIPTION

[0014] The utility model will be further described below in conjunction with the accompanying drawings.

[0015] like Figure 1 , 2 As shown, a transwell chamber scraper device comprises a chamber body 1, a semipermeable membrane 3 arranged at the bottom of the chamber body 1 and a scraper 2 matched with the chamber body 1. The chamber body 1 comprises an inverted conical tube 11 and an annular outer edge 12 arranged at the upper end of the inverted conical tube 11, and the chamber body 1 is a common transwell chamber structure. The scraper 2 includes a scraper 21 that is tightly attached to the bottom of the chamber body 1 and a hand-pushing part connected to the scraper 21; the scraper 21 is in an arc shape, and the bubbles collected by the scraper 21 when translated can be gathered together, which is more convenient for cleaning later, and a collecting groove can even be set on the concave surface of the scraper 21, and the bubbles can be collected in the collecting groove when the scraper 21 translates over the bubbles. After the bubbles are collected, the scraper 21 can be separated from the chamber body 1; the two ends of the scraper 21 are respectively connected to the slide rails 24, and the two slide rails 24 are arranged in parallel. The cross-section of the slide rails 24 is convex, and the two sides of the bottom of the chamber body 1 are provided with slide grooves 23 corresponding to the slide rails 24, and the upper part of the slide rail 24 extends into the slide groove 23 and slides with it, through the slide rails 24 and The design of the slide groove 23 can enhance the stability of the translation of the scraper 21; one end of the two slide rails 24 is connected by a horizontal connecting rod 25 to form a sliding frame, and the top surface of the chamber body 1 is provided with a push rod 22 that is in contact with the annular outer edge 12, and the two ends of the push rod 22 are connected to the sliding frame through vertical connecting rods 26. The push rod 22 is designed at the top of the chamber body 1 to facilitate manual operation. The structural strength of the sliding frame is higher to prevent the scraper 21 from shifting during the translation process; the upper end of the vertical connecting rod 26 is provided with a bent avoidance groove 27, and the annular outer edge 12 can pass through the avoidance groove 27. The design of the avoidance groove 27 can enable the entire scraper 2 to move from one side of the chamber body 1 to the other side, or even completely separate the scraper 2 from the chamber body 1.

[0016] Principle of the utility model: in the initial state, the scraper 21 is located at one side of the bottom of the chamber body 1. When it is necessary to clean the bubbles under the semipermeable membrane 3, the hand pusher is used to drive the scraper 21 so that the scraper 21 moves horizontally from one side of the chamber body 1 to the other side. When the scraper 21 passes under the semipermeable membrane 3, the bubbles are collected together and moved out of the bottom of the chamber body 1. At this time, the bubbles collected by the scraper 21 can be conveniently sucked away by the gun head, which is quick and convenient.

Claims

1. A transwell chamber scraper device, comprising a chamber body and a semipermeable membrane arranged at the bottom of the chamber body, wherein the chamber body comprises an inverted conical tube and an annular outer edge arranged at the upper end of the inverted conical tube, characterized in that: It also includes a scraper matched with the chamber body, and the scraper includes a scraper plate closely attached to the bottom of the chamber body and a hand-pushing part connected with the scraper plate.

2. A transwell chamber scraper device according to claim 1, characterized in that: The scraper is in an arc shape.

3. A transwell chamber scraper device according to claim 1, characterized in that: The two ends of the scraper are respectively connected to the slide rails, and the two sides of the bottom of the chamber body are provided with slide grooves, and the slide rails are slidably matched with the slide grooves.

4. A transwell chamber scraper device according to claim 3, characterized in that: One ends of the two slide rails are connected through a transverse connecting rod to form a sliding frame, and the top surface of the small chamber body is provided with a push rod in contact with the annular outer edge, and the two ends of the push rod are connected to the sliding frame through a vertical connecting rod.

5. A transwell chamber scraper device according to claim 4, characterized in that: The upper end of the vertical connecting rod is provided with a bending avoidance groove, and the annular outer edge can pass through the avoidance groove.

6. A transwell chamber scraper device according to claim 1, characterized in that: A collecting groove is arranged on the inner side of the scraper.

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