Combined porous culture plate

Abstract

The utility model relates to the technical field of biological cell culture, and discloses a combined porous culture plate, which comprises a pore plate frame and a plurality of pore plate units, the pore plate frame is in a hollow ring shape, the pore plate units are detachably arranged in a hollow cavity of the pore plate frame, and a plurality of culture holes arranged in a matrix are arranged on the pore plate units; the volumes of the culture holes in a single pore plate unit are the same, and the volumes of the culture holes in different pore plate units are the same or different. The pore plate units are detachably connected with the pore plate frame, the number of the culture holes can be adjusted by adjusting the column number or the row number of the pore plate units assembled on the pore plate frame, the pore plate units with different volumes can be spliced together, and the number of the culture holes can be adjusted according to the number of organoid for high-flux drug sensitivity detection. The pore plate units with the corresponding quantity and the corresponding volume are selected, so that waste of culture pores is avoided. The pore plate units of the culture pores with different pore volumes can be combined together for different research purposes, the use is flexible, and diversified experiment requirements are met.

Description

Technical Field

[0001] The utility model relates to the technical field of biological cell culture, in particular to a combined multi-hole culture plate. Background Art

[0002] Currently, the 384-well plates sold on the market include fully transparent plates, pure white plates, pure black plates, and white-walled or black-walled bottom transparent plates, which are used for PCR, cell culture, fluorescence detection, chemiluminescence detection, etc. Among them, the 384-well plates used for high-throughput drug screening of organoids are mainly white-walled or black-walled bottom transparent plates. The transparent bottom design facilitates observation of cell status, while the white or black walls can be used for chemiluminescence detection and fluorescence detection methods used in high-throughput drug screening of organoids. The white-walled or black-walled bottom transparent 384-well plates on the market are all deep-well plates with a well volume of 100μL to 120μL. The working volume is generally recommended to be 20μL to 80μL, and there are greater requirements for the number of cells to be inoculated. Organoid samples come from tissues or stem cells, which are relatively precious. The amount of original cells before high-throughput drug screening is limited. In the currently available 384-well plates, organoids need a certain inoculation density to grow. When the number of organoids is small, only a few compounds or drugs can be tested; the entire 384-well plate is a whole and cannot be freely integrated. That is, if only 100 wells are used, the remaining wells will be wasted or contaminated during use. In addition, the culture conditions of each organoid sample are different, and sometimes not all the culture wells of the 384-well plate are needed.

[0003] In view of the special requirements and specific uses of high-throughput drug screening of organoids, it is necessary to design a multi-well culture plate that can flexibly adjust the number and volume of culture wells. Utility Model Content

[0004] In order to solve the above-mentioned deficiencies in the prior art, the utility model provides a modular multi-well culture plate that can freely assemble the number and volume of culture holes.

[0005] In order to achieve the above technical purpose, the technical solution adopted by the utility model is:

[0006] A combined multi-well culture plate comprises a well plate frame and a plurality of well plate units, wherein the well plate frame is in a hollow ring shape, the well plate units are detachably mounted in the hollow cavity of the well plate frame, and the well plate units are provided with a plurality of culture wells arranged in a matrix; the volumes of the culture wells on a single well plate unit are the same, and the volumes of the culture wells on different well plate units are the same or different.

[0007] Furthermore, the culture wells of different volumes have different upper surface areas and / or bottom surface areas.

[0008] Preferably, culture wells of different volumes have different depths.

[0009] Preferably, the volume range of the culture well is 10 μL to 120 μL.

[0010] Furthermore, the upper surface and the bottom surface of the culture well are both square or circular.

[0011] Furthermore, the area of ​​the bottom surface of the culture hole is less than or equal to the area of ​​the upper surface, and the bottom surface is a plane.

[0012] Furthermore, the side wall of the culture well is white or black, and the bottom is transparent.

[0013] Furthermore, the orifice plate frame is square, and at least one group of opposite edge top surfaces of the orifice plate frame are linearly arranged with a plurality of buckles, and both ends of the orifice plate unit are provided with through slots, the slots are adapted to the buckles, and the orifice plate unit is snap-connected with the orifice plate frame.

[0014] Furthermore, the orifice plate unit includes a short orifice plate unit and / or a long orifice plate unit, the short orifice plate unit is installed on the orifice plate frame in a direction parallel to the short side of the orifice plate frame, and the long orifice plate unit is installed on the orifice plate frame in a direction parallel to the long side of the orifice plate frame, and the number of culture wells on the short orifice plate unit and the long orifice plate unit are different.

[0015] Preferably, the orifice plate frame is evenly provided with 16 groups of clips along the two short sides, connecting up to 16 long-hole plate units, and 24 culture holes are evenly provided on the long-hole plate units; or: the orifice plate frame is evenly provided with 24 groups of clips along the two long sides, connecting up to 24 short-hole plate units, and 16 culture holes are evenly provided on the short-hole plate units.

[0016] The beneficial effects of the utility model are:

[0017] The utility model provides a combined multi-well culture plate, in which the well plate unit is detachably connected to the well plate frame, and the number of culture wells can be adjusted by adjusting the number of columns or rows of the well plate units assembled on the well plate frame, and well plate units of different volumes can also be pieced together. The entire well plate frame does not have to be filled, and can be arranged according to actual conditions. This allows the well plate units of a corresponding number and volume to be selected according to the number of organoids for high-throughput drug sensitivity testing, thereby avoiding waste of culture wells. The same well plate frame can also combine well plate units of culture wells of different hole volumes for different research purposes to meet diverse experimental needs. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings required for use in the embodiments will be briefly introduced below. It should be understood that the following drawings only show certain embodiments of the present application and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other related drawings can be obtained based on these drawings without paying creative work.

[0019] Figure 1 It is a schematic diagram of the overall structure of an embodiment of the utility model;

[0020] Figure 2 This is a schematic diagram of the structure of the culture hole in the embodiment of the utility model;

[0021] Figure 3 It is a structural schematic diagram of the orifice plate unit;

[0022] Figure 4 It is another structural schematic diagram of the orifice plate unit;

[0023] Figure 5 It is a schematic diagram of the connection between the long hole plate unit and the hole plate frame;

[0024] Figure 6 It is a schematic diagram of the connection between the short hole plate unit and the hole plate frame.

[0025] Figure numerals: 1-well plate frame, 2-well plate unit, 3-culture well, 4-clip, 5-slot. DETAILED DESCRIPTION

[0026] To make the purpose, technical scheme and advantages of the embodiments of the present application clearer, the technical scheme in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application. Obviously, the described embodiments are part of the embodiments of the present application, rather than all of the embodiments. The components of the embodiments of the present application generally described and shown in the drawings here can be arranged and designed in various different configurations. Therefore, the following detailed description of the embodiments of the present application provided in the drawings is not intended to limit the scope of the application claimed for protection, but merely represents the selected embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians of the art without making creative work are within the scope of protection of the present application.

[0027] A combined multi-well culture plate, such as Figure 1-Figure 4 As shown, it includes a well plate frame 1 and a plurality of well plate units 2, wherein the well plate frame 1 is in a hollow ring shape, and the well plate unit 2 can be detachably installed in the hollow cavity of the well plate frame 1, and the well plate unit 2 is provided with a plurality of culture wells 3 arranged in a matrix, the volume of the culture wells on a single well plate unit 2 is the same, and the volume of the culture wells on different well plate units 2 is the same or different.

[0028] The upper surface area and / or bottom surface area of ​​the culture wells of different volumes are different, or the depth of the culture wells is different. The volume range of the culture wells is 10 μL to 120 μL, and the upper surface area of ​​the culture wells is ≤ 12.25 mm 2 , the depth of the culture well is 5 mm to 11 mm. Furthermore, the upper surface and the bottom surface of the culture well are both square or circular, wherein the area of ​​the bottom surface is less than or equal to the area of ​​the upper surface, and the bottom surface is a plane, that is, the side wall of the culture well is perpendicular to the bottom surface, or the angle between the side wall and the bottom surface is greater than 90°, Figure 2 The area of ​​the bottom surface of the culture hole is smaller than that of the top surface, and the inner wall is in an inverted cone shape. The side wall of the culture hole 3 is white or black, and the bottom surface is transparent.

[0029] In one embodiment, the orifice plate frame 1 is square, and at least one group of opposite sides of the orifice plate frame 1 is linearly arranged with a plurality of buckles 4, and both ends of the orifice plate unit 2 are provided with through-grooves 5, and the slots 5 are adapted to the buckles 4, and the orifice plate unit 2 is snap-connected with the orifice plate frame 1 to prevent the orifice plate unit from falling during use. The orifice plate unit 2 includes a short orifice plate unit and / or a long orifice plate unit, and the short orifice plate unit is installed on the orifice plate frame 1 in a direction parallel to the short side of the orifice plate frame 1, and the long orifice plate unit is installed on the orifice plate frame 1 in a direction parallel to the long side of the orifice plate frame 1, and the number of culture wells on the short orifice plate unit and the long orifice plate unit is different. Taking a 384-well plate as an example, the well plate frame is evenly arranged with 16 sets of buckles along the two short sides, connecting up to 16 long-well plate units, and 24 culture wells are evenly arranged on the long-well plate units; or: the well plate frame is evenly arranged with 24 sets of buckles along the two long sides, connecting up to 24 short-well plate units, and 16 culture wells are evenly arranged on the short-well plate units. According to experimental needs, a suitable number of short-well plate units or long-well plate units can be selected and installed on the well plate frame 1, and short-well plate units or long-well plate units of different volumes can also be assembled together. The entire 384-well plate does not have to be filled, and can be arranged according to actual conditions.

[0030] Experimental Example 1:

[0031] The cultured organoids were digested into single cells by digestive enzymes, and the cell count was 8×10 4 , select a short-well plate unit with a well volume of 81 μL, the top and bottom surfaces of the culture wells are square, and the top and bottom areas are 9 mm 2 , 9 mm deep, flat bottom and transparent bottom. Resuspend the organoids in the corresponding volume of culture medium at a seeding density of 800 cells per well, and assemble 6 rows of the above 81 μL short-well plate units in the well border of a 384-well culture plate, such as Figure 5As shown, the organoids were inoculated into 96 culture wells at a volume of 20 μL per well. The appropriate inoculation density was observed under a microscope. Drugs were then added and the effects of the drugs on the organoids were observed under a microscope at different time points. After the drugs acted for a certain period of time, the activity of the organoids was measured at different drug concentrations, and the drug sensitivity data of the organoids were analyzed.

[0032] Experimental Example 2:

[0033] The cultured organoids were digested into single cells by digestive enzymes, and the cell count was 4×10 4 , select a long-well plate unit with a pore volume of 39.25 μL, the upper and lower surfaces of the culture well are circular, and the upper and lower surface areas are 4.9 mm 2 , 8 mm deep, flat bottom and transparent bottom. Resuspend the organoids in the corresponding volume of culture medium according to the seeding density of 400 cells per well, and assemble 4 rows of the above 39.25 μL long-well plate units in the well plate border of the 384-well culture plate, such as Figure 6 As shown, the organoids were inoculated into 96 culture wells at a volume of 10 μL per well, and the appropriate inoculation density was observed under a microscope. Then the drugs were added, and the effects of the drugs on the organoids at different time points were observed under a microscope. After the drugs acted for a certain period of time, the activity of the organoids at different drug concentrations was measured, and the drug sensitivity data of the organoids were analyzed.

[0034] Of course, the present invention may have many other embodiments. Without departing from the spirit and essence of the present invention, technicians familiar with the field may make various corresponding changes and deformations based on the present invention, but these corresponding changes and deformations should all fall within the scope of protection of the claims attached to the present invention.

Claims

1. A combined multi-well culture plate, comprising a well plate frame and a plurality of well plate units, wherein the well plate frame is in a hollow ring shape, the well plate units are detachably mounted in the hollow cavity of the well plate frame, and the well plate units are provided with a plurality of culture wells arranged in a matrix; the volumes of the culture wells on a single well plate unit are the same, and the volumes of the culture wells on different well plate units are the same or different.

2. The combined multi-well culture plate according to claim 1, characterized in that: The culture wells of different volumes have different upper surface areas and / or bottom surface areas.

3. The combined multi-well culture plate according to claim 1 or 2, characterized in that: The culture wells of different volumes have different depths.

4. The combined multi-well culture plate according to claim 1, characterized in that: The volume range of the culture well is 10 μL to 120 μL.

5. The combined multi-well culture plate according to claim 4, characterized in that: The upper surface and the bottom surface of the culture hole are both square or circular.

6. The combined multi-well culture plate according to claim 5, characterized in that: The area of ​​the bottom surface of the culture hole is less than or equal to the area of ​​the upper surface, and the bottom surface is a plane.

7. The combined multi-well culture plate according to claim 1, characterized in that: The side wall of the culture hole is white or black, and the bottom surface is transparent.

8. The combined multi-well culture plate according to claim 1, characterized in that: The orifice plate frame is square, and at least one group of opposite edge top surfaces of the orifice plate frame are linearly arranged with a plurality of buckles. Both ends of the orifice plate unit are provided with through slots, which are adapted to the buckles, and the orifice plate unit is snap-connected with the orifice plate frame.

9. The combined multi-well culture plate according to claim 8, characterized in that: The orifice plate unit includes a short orifice plate unit and / or a long orifice plate unit. The short orifice plate unit is installed on the orifice plate frame in a direction parallel to the short side of the orifice plate frame, and the long orifice plate unit is installed on the orifice plate frame in a direction parallel to the long side of the orifice plate frame. The number of culture wells on the short orifice plate unit and the long orifice plate unit is different.

10. The combined multi-well culture plate according to claim 9, characterized in that: The orifice plate frame is evenly arranged with 16 groups of buckles along the two short sides, connecting up to 16 long-hole plate units, and 24 culture holes are evenly arranged on the long-hole plate units; or: the orifice plate frame is evenly arranged with 24 groups of buckles along the two long sides, connecting up to 24 short-hole plate units, and 16 culture holes are evenly arranged on the short-hole plate units.

Images