Stem cell exosome preservation device

Abstract

The utility model discloses a stem cell exosome preservation device which comprises a heat preservation box, a fixed partition plate is arranged at the bottom of the interior of the heat preservation box, heat conduction plates are fixedly installed at the top of the fixed partition plate, heat conduction arc frames are integrally arranged on the opposite faces of the adjacent heat conduction plates, and heat conduction metal sleeves are fixedly installed between the heat conduction arc frames. A test tube is arranged in the heat conduction metal sleeve, a semiconductor chilling plate is arranged at the bottom of the fixed partition plate, a heat pipe making contact with the semiconductor chilling plate is laid in the heat conduction plate, and a cooling fan is arranged at the bottom of the heat preservation box. After the cover of the heat preservation box is opened, the influence of temperature change in the heat preservation box on the heat conduction metal sleeve is small, so that the test tubes for storing the stem cell exosomes in the heat conduction metal sleeve cannot be changed due to opening of the cover of the heat preservation box.

Description

Technical Field

[0001] The utility model relates to the technical field of stem cell preservation, in particular to a stem cell exosome preservation device. Background Art

[0002] Exosomes refer to small membrane vesicles (30-150nm) containing complex RNA and proteins. Nowadays, they specifically refer to disc-shaped vesicles with a diameter of 40-100nm. A variety of cells can secrete exosomes under normal and pathological conditions. They are mainly derived from multivesicular bodies formed by the invagination of intracellular lysosomal microparticles, and are released into the extracellular matrix after the fusion of the multivesicular body membrane with the cell membrane.

[0003] There are some problems with today's stem cell exosome storage devices. First, because exosomes can resist dissolution by non-ionic detergents at low temperatures and protect the contents from degradation, exosome operations should be performed as much as possible in a low-temperature environment and the number of repeated freeze-thaw cycles should be reduced. Traditional stem cell exosome storage technology uses multiple test tubes to store and take out at the same time. In the process of taking a test tube, all test tubes need to be exposed to room temperature, which reduces the storage effect of stem cells.

[0004] To this end, we propose a stem cell exosome preservation device. Utility Model Content

[0005] The purpose of the utility model is to provide a stem cell exosome storage device to solve the problems raised in the above background technology.

[0006] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a stem cell exosome preservation device, comprising an incubator, a fixed partition is provided at the bottom of the incubator, a heat-conducting plate is fixedly installed on the top of the fixed partition, heat-conducting arc frames are integrally provided on the opposite surfaces of adjacent heat-conducting plates, heat-conducting metal sleeves are fixedly installed between the heat-conducting arc frames, test tubes are provided inside the heat-conducting metal sleeves, a semiconductor refrigeration sheet is provided at the bottom of the fixed partition, a heat pipe in contact with the semiconductor refrigeration sheet is laid inside the heat-conducting plate, and a cooling fan is provided at the bottom of the incubator.

[0007] Optionally, a sealed box is fixedly installed on the bottom of the fixed partition, the semiconductor refrigeration sheet is fixedly installed on the bottom of the sealed box, and the cooling surface of the semiconductor refrigeration sheet faces upward.

[0008] Optionally, the heat pipe includes a coiled portion laid inside the heat conductive plate, and a horizontal portion located below the fixed partition, the bottom of the horizontal portion of the heat pipe is in close contact with the top of the semiconductor refrigeration plate, and the heat pipe passes through the fixed partition.

[0009] Optionally, the heat-conducting metal sleeve, the heat-conducting plate and the fixed partition are made of the same material, a metal cover made of the same material is provided on the top of the heat-conducting metal sleeve, and the thickness of the heat-conducting metal sleeve is greater than the thickness of the heat-conducting plate.

[0010] Optionally, the cooling fan is located directly below the semiconductor refrigeration sheet, and heat dissipation holes are provided on the fixing frame of the cooling fan and the lower part of the heat preservation box.

[0011] Optionally, the bottom of the insulated box is provided with supporting feet, and four of the supporting feet are respectively fixedly installed on the four corners of the bottom of the insulated box.

[0012] Optionally, heat transfer oil is filled between the sealing box and the fixed partition.

[0013] Compared with the prior art, the beneficial effects of the utility model are:

[0014] 1. The stem cell exosome preservation device is provided with a plurality of heat-conducting arc frames, and the cooling surface of the semiconductor refrigeration sheet is used for cooling and heat is exchanged with the inside of the incubator and the heat-conducting metal sleeve through the fixed partition and the heat pipe, so that the temperature inside the incubator is maintained at the optimum temperature. After the cover of the incubator is opened, the temperature change inside the incubator has little effect on the heat-conducting metal sleeve, so that the test tubes storing stem cell exosomes inside the heat-conducting metal sleeve will not change due to opening the cover of the incubator.

[0015] 2. The stem cell exosome preservation device, by setting fixed partitions, heat conduction plates and heat pipes, allows the heat inside the incubator to be transferred to the semiconductor refrigeration plate in a variety of ways, and the cooling fan and heat dissipation holes are set to dissipate the heat surface of the semiconductor refrigeration plate. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a schematic diagram of the overall structure of a stem cell exosome storage device of the utility model;

[0017] Figure 2 This is a schematic diagram of the structure of an incubator box of a stem cell exosome storage device of the utility model;

[0018] Figure 3 This is a schematic diagram of the structure of a heat-conducting plate of a stem cell exosome storage device of the utility model;

[0019] Figure 4 This is a schematic diagram of the structure of a semiconductor cooling plate of a stem cell exosome storage device of the utility model.

[0020] In the figure: 1. Insulation box; 2. Fixed partition; 3. Heat conduction plate; 4. Heat conduction arc frame; 5. Heat conduction metal sleeve; 6. Test tube; 7. Sealed box; 8. Semiconductor refrigeration plate; 9. Cooling fan; 10. Heat dissipation hole; 11. Heat pipe; 1101. Horizontal part; 1102. Coiled part; 12. Support foot. DETAILED DESCRIPTION

[0021] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0022] See also Figures 1 to 4 The utility model provides a stem cell exosome preservation device, including an insulated box 1, a fixed partition 2 is arranged at the bottom of the insulated box 1, a heat conducting plate 3 is fixedly installed on the top of the fixed partition 2, a heat conducting arc frame 4 is integrally arranged on the opposite surfaces of adjacent heat conducting plates 3, a heat conducting metal sleeve 5 is fixedly installed between the heat conducting arc frames 4, a test tube 6 is arranged inside the heat conducting metal sleeve 5, a semiconductor refrigeration sheet 8 is arranged at the bottom of the fixed partition 2, a heat pipe 11 in contact with the semiconductor refrigeration sheet 8 is laid inside the heat conducting plate 3, and a cooling fan 9 is arranged at the bottom of the insulated box 1. By arranging a plurality of heat conducting arc frames 4, the cooling surface of the semiconductor refrigeration sheet 8 is cooled and heat is exchanged with the inside of the insulated box 1 and the heat conducting metal sleeve 5 through the fixed partition 2 and the heat pipe 11, so that the temperature inside the insulated box 1 is maintained at the optimum temperature. After the cover of the insulated box 1 is opened, the temperature change inside the insulated box 1 has little effect on the heat conducting metal sleeve 5, so that the test tube 6 storing the stem cell exosome inside the heat conducting metal sleeve 5 will not change due to opening the cover of the insulated box 1.

[0023] A sealed box 7 is fixedly installed at the bottom of the fixed partition 2, and a semiconductor refrigeration sheet 8 is fixedly installed at the bottom of the sealed box 7, with the cooling surface of the semiconductor refrigeration sheet 8 facing upward.

[0024] The heat pipe 11 includes a coiled portion 1102 laid inside the heat conducting plate 3 and a horizontal portion 1101 located below the fixed partition 2 . The bottom of the horizontal portion 1101 of the heat pipe 11 is in close contact with the top of the semiconductor cooling plate 8 , and the heat pipe 11 passes through the fixed partition 2 .

[0025] The heat-conducting metal sleeve 5, the heat-conducting plate 3 and the fixed partition 2 are made of the same material. A metal cover made of the same material is provided on the top of the heat-conducting metal sleeve 5. The thickness of the heat-conducting metal sleeve 5 is greater than that of the heat-conducting plate 3. By providing the fixed partition 2, the heat-conducting plate 3 and the heat pipe 11, the heat inside the thermal insulation box 1 is transferred to the semiconductor refrigeration plate 8 in a variety of ways.

[0026] The cooling fan 9 is located directly below the semiconductor cooling sheet 8 . The fixing frame of the cooling fan 9 and the lower half of the heat preservation box 1 are provided with heat dissipation holes 10 . The cooling fan 9 and the heat dissipation holes 10 are provided to dissipate heat from the hot surface of the semiconductor cooling sheet 8 .

[0027] The bottom of the heat preservation box 1 is provided with supporting feet 12 , and four supporting feet 12 are fixedly installed at four corners of the bottom of the heat preservation box 1 respectively.

[0028] The space between the sealing box 7 and the fixed partition plate 2 is filled with heat transfer oil.

[0029] Working principle:

[0030] By setting up multiple heat-conducting arc frames 4, the cooling surface of the semiconductor refrigeration plate 8 is cooled and heat is exchanged with the inside of the insulation box 1 and the heat-conducting metal sleeve 5 through the fixed partition 2 and the heat pipe 11, so that the fixed partition 2, the heat-conducting plate 3 and the heat pipe 11, so that the heat inside the insulation box 1 is transferred to the semiconductor refrigeration plate 8 in various ways, and the temperature inside the insulation box 1 is maintained at the optimum temperature. After the lid of the insulation box 1 is opened, the temperature change inside the insulation box 1 has little effect on the heat-conducting metal sleeve 5, so that the test tube 6 storing the stem cell exosomes inside the heat-conducting metal sleeve 5 will not change due to opening the lid of the insulation box 1, and the cooling fan 9 and the heat dissipation hole 10 dissipate heat from the hot surface of the semiconductor refrigeration plate 8.

[0031] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to these embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A stem cell exosome storage device, comprising an incubator (1), characterized in that: A fixed partition (2) is provided at the bottom of the thermal insulation box (1), a heat conducting plate (3) is fixedly installed on the top of the fixed partition (2), heat conducting arc frames (4) are integrally provided on the opposite surfaces of adjacent heat conducting plates (3), a heat conducting metal sleeve (5) is fixedly installed between the heat conducting arc frames (4), a test tube (6) is provided inside the heat conducting metal sleeve (5), a semiconductor cooling plate (8) is provided at the bottom of the fixed partition (2), a heat pipe (11) in contact with the semiconductor cooling plate (8) is laid inside the heat conducting plate (3), a cooling fan (9) is provided at the bottom of the thermal insulation box (1), the heat conducting metal sleeve (5), the heat conducting plate (3) and the fixed partition (2) are made of the same material, a metal cover made of the same material is provided on the top of the heat conducting metal sleeve (5), and the thickness of the heat conducting metal sleeve (5) is greater than the thickness of the heat conducting plate (3).

2. A stem cell exosome storage device according to claim 1, characterized in that: A sealed box (7) is fixedly mounted on the bottom of the fixed partition (2), and the semiconductor refrigeration sheet (8) is fixedly mounted on the bottom of the sealed box (7), with the refrigeration surface of the semiconductor refrigeration sheet (8) facing upward.

3. A stem cell exosome storage device according to claim 2, characterized in that: The heat pipe (11) comprises a coiled portion (1102) laid inside the heat conducting plate (3), and a horizontal portion (1101) located below the fixed partition (2); the bottom of the horizontal portion (1101) of the heat pipe (11) is in tight contact with the top of the semiconductor refrigeration plate (8), and the heat pipe (11) passes through the fixed partition (2).

4. A stem cell exosome storage device according to claim 1, characterized in that: The cooling fan (9) is located directly below the semiconductor refrigeration sheet (8), and a fixing frame of the cooling fan (9) and a lower part of the heat preservation box (1) are provided with heat dissipation holes (10).

5. A stem cell exosome storage device according to claim 1, characterized in that: The bottom of the thermal insulation box (1) is provided with supporting feet (12), and the four supporting feet (12) are respectively fixedly mounted on the four corners of the bottom of the thermal insulation box (1).

6. A stem cell exosome storage device according to claim 2, characterized in that: The space between the sealing box (7) and the fixed partition plate (2) is filled with heat transfer oil.

Images