Pleurotus geesteranus mother strain preservation pipe
By designing the Xiuzhen mushroom mother seed storage tube, including a conical feeding nozzle and sealing sleeve, the problems of cumbersome operation and the fall of cotton balls are solved, and the effect of convenient feeding and protecting the growth environment of mycelium is achieved.
Patent Information
- Application Number
- CN202421197114.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-05-29
- Publication Date
- 2025-02-25
- Estimated Expiration
- 2034-05-29
AI Technical Summary
In the prior art, when using a centrifuge tube for the storage method of Xiuzhen mushroom mother seeds, it is inconvenient to manually open the mother seeds, and the culture material is prone to leak out; at the same time, it is easy to fall into the respiratory area after the top of the centrifuge tube is stuffed into a cotton ball, affecting the mother. growth of mycelium.
A kind of Xiuzhen Mushroom mother-type storage tube is designed, including the tube body, top cover, material pickup nozzle and sealing sleeve. The material retrieval nozzle is cone-shaped, and a tubular material retrieval opening is extended from the bottom. A plug is provided in the center of the sealing sleeve to extend into the material retrieval opening. The inner wall of the tube body is evenly equipped with a limiting block, and the limiting groove of the limiting block and the sealing sleeve cooperate with the limiting ring of the material take-off nozzle to prevent the sealing sleeve from slipping off.
It solves the problem that traditional centrifuge tubes require manual opening and sealing operations, avoids leakage of culture materials, and prevents the cotton ball from falling into the respiratory area, protecting the growth environment of the mother mycelium.
Smart Images
Figure CN222524543U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of bacterial strain culture devices, in particular to a Pleurotus geesteranus mother strain preservation tube. Background Art
[0002] Pleurotus geesteranus, also known as pocket mushroom, has low fiber content, excellent taste and delicious flavor. The mother strain of Pleurotus geesteranus cultivated in the laboratory needs to be preserved for subsequent expansion.
[0003] At present, the preservation method of Pleurotus geesteranus mother strain is to use centrifuge tubes for preservation. Culture medium is added into the centrifuge tubes, and then dust-proof cotton balls are plugged into the tube mouths and disinfected and sterilized. After sterilization, Pleurotus geesteranus strains are inoculated into the culture medium of the centrifuge tubes in an inoculation room, and then the tubes are covered with a cap and put into a preservation box for preservation. When expansion cultivation is required, the centrifuge tubes are taken out. In order to reduce the contact area between the material taking area and the outside world, a small hole needs to be opened at the tip of the bottom of the centrifuge tube. After taking out the required amount of mother strains with a dissecting needle, the small hole is sealed with a plastic film and then put into the preservation box for preservation.
[0004] At present, there are the following problems when using centrifuge tubes as storage tubes to store Pleurotus geesteranus mother strains: first, a small opening needs to be opened at the tip of the bottom of the centrifuge tube during expansion, and the mother strain needs to be taken out and then sealed with a plastic film, which is not only inconvenient to operate, but also the culture material at the bottom is easy to leak out after the opening; second, a section of space needs to be reserved in the middle of the storage tube as a breathing zone, but currently, after the cotton ball is stuffed into the top of the centrifuge tube, it is easy to fall into the tube section of the breathing zone, which affects the growth of the mother strain mycelium. Therefore, the present application proposes a Pleurotus geesteranus mother strain storage tube. Utility Model Content
[0005] In order to overcome the problems in the background technology, the utility model provides a storage tube for Pleurotus geesteranus mother cultures, which solves the problems that when centrifuge tubes are currently used as storage tubes for preserving Pleurotus geesteranus mother cultures, an opening needs to be opened when taking out the mother cultures, which is inconvenient to operate, the culture medium is easy to leak out, and a cotton ball inserted into the top of the centrifuge tube is easy to fall into the tube section of the breathing zone, thereby affecting the growth of the mother culture mycelium.
[0006] A storage tube for Pleurotus geesteranus mother seeds comprises a tube body, a top cover, a feeding nozzle and a sealing sleeve; the top of the tube body is connected to the top cover, the bottom of the tube body is provided with a feeding nozzle and is integrally formed with the tube body, the feeding nozzle is conical, the bottom of the feeding nozzle extends to form a tubular feeding port, the sealing sleeve is installed with the outer wall of the feeding port, and a plug is provided in the center of the sealing sleeve to extend into the feeding port.
[0007] Furthermore, the material taking opening is eccentrically arranged at the bottom of the material taking nozzle, so that the material taking nozzle and a side wall of the tube body are connected in a straight line.
[0008] Furthermore, limit blocks are evenly arranged on the inner wall of the tube body, and the limit blocks are arranged in the middle and upper part of the inner wall of the tube body.
[0009] Furthermore, a limiting groove is provided on the inner wall of the sealing sleeve to cooperate with a limiting ring provided on the outer wall of the material taking nozzle.
[0010] Compared with the prior art, the beneficial effects of the utility model are:
[0011] The present application is provided with a feeding nozzle, on which a sealing sleeve is installed, and a plug is provided in the center of the sealing sleeve and extends into the feeding port, which not only solves the problem that the traditional centrifuge tube needs to be opened manually with a scalpel and sealed with a plastic film, which is cumbersome to operate, but also the plug can fill the feeding port, thereby avoiding the problem that the culture medium is overfilled and the culture medium is easy to leak out after the feeding port is opened; limiting blocks are evenly arranged on the inner wall of the tube body of the present application, which can solve the problem that the cotton ball easily falls into the pipe section of the breathing zone after being stuffed into the top of the centrifuge tube. BRIEF DESCRIPTION OF THE DRAWINGS
[0012] In order to clearly illustrate the technical solutions in the embodiments of the present utility model, the drawings required for use in describing the embodiments are described.
[0013] Figure 1 It is a schematic diagram of the overall structure of the utility model;
[0014] Figure 2 It is a schematic diagram of the internal structure of the utility model;
[0015] Figure 3 It is a schematic diagram of the structure of the material taking nozzle of the utility model.
[0016] 1-tube body, 11-limiting block, 2-top cover, 3-feeding nozzle, 31-feeding port, 32-limiting ring, 4-sealing sleeve, 41-plug block, 42-limiting groove. DETAILED DESCRIPTION
[0017] In order to make the purpose, technical solution and beneficial effects of the present invention clearer, the preferred embodiments of the present invention will be described in detail below in conjunction with the accompanying drawings to facilitate understanding by technicians.
[0018] The utility model discloses a storage tube for mother seeds of Pleurotus geesteranus. Figure 1-3 A storage tube for Pleurotus geesteranus mother seeds comprises a tube body 1, a top cover 2, a feeding nozzle 3 and a sealing sleeve 4; the top of the tube body 1 is connected to the top cover 2, the bottom of the tube body 1 is provided with a feeding nozzle 3 and is integrally formed with the tube body 1, the feeding nozzle 3 is conical, and the bottom of the feeding nozzle 3 extends to form a tubular feeding port 31, the sealing sleeve 4 is installed with the outer wall of the feeding port 31, and a plug 41 is provided in the center of the sealing sleeve 4 to extend into the feeding port 31.
[0019] The tube body 1 and the feeding nozzle 3 can be filled with culture medium of the mother strain of Pleurotus geesteranus, the top cover 2 can seal the open opening on the top of the tube body 1, and the sealing sleeve 4 can be put on the feeding port 31 of the feeding nozzle 3 to seal it, which solves the problem that the traditional centrifuge tube needs to be opened manually with a scalpel and sealed with a plastic film, which is cumbersome to operate. The plug 41 of the sealing sleeve 4 can be inserted into the feeding port 31 to fill the feeding port 31, thereby avoiding the problem that the culture medium fills the feeding port 31 and easily leaks out after it is opened.
[0020] See also Figure 1-3 The feeding port 31 is eccentrically arranged at the bottom of the feeding nozzle 3, so that the feeding nozzle 3 and the side wall of the tube body 1 are connected in a straight line, which is convenient for the dissecting needle to remove the mother culture mycelium and avoids the problem that the traditional conical feeding nozzle easily leaks out when taking materials vertically and easily accumulates in the inclined groove when taking materials horizontally.
[0021] See also Figure 1-3 The inner wall of the tube body 1 is evenly provided with limit blocks 11, and the limit blocks 11 are arranged in the middle and upper part of the inner wall of the tube body 1, so as to solve the problem that the cotton ball is easily dropped into the tube section of the breathing zone after being stuffed into the top of the traditional centrifuge tube.
[0022] See also Figure 1-3 The inner wall of the sealing sleeve 4 is provided with a limiting groove 42 which cooperates with the limiting ring 32 provided on the outer wall of the material taking nozzle 3 to prevent the sealing sleeve 4 from slipping.
[0023] Working process:
[0024] Add culture medium into the tube body 1, then plug a dust-proof cotton ball into the tube mouth and disinfect and sterilize it. After sterilization, inoculate the culture medium of the tube body 1 in the inoculation room, then cover it with the top cover 2 and put it into the preservation box for preservation. When it is necessary to expand the culture, take out the tube body 1, open the sealing sleeve 4 on the material taking mouth 3, move the plug 41 out from the material taking mouth 31, take out the required amount of mother culture from the material taking mouth 31 with a dissecting needle, cover it with the sealing sleeve 4 and put it into the preservation box for preservation.
[0025] What is described above is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes within the technical scope disclosed by the present invention according to the technical scheme and the utility model concept of the present invention, which should be covered by the protection scope of the present invention.
Claims
1. A storage tube for Pleurotus geesteranus mother seeds, characterized in that: The invention comprises a tube body (1), a top cover (2), a material taking nozzle (3) and a sealing sleeve (4); the top of the tube body (1) is connected to the top cover (2); the bottom of the tube body (1) is provided with a material taking nozzle (3) and is integrally formed with the tube body (1); the material taking nozzle (3) is conical; the bottom of the material taking nozzle (3) extends to form a tubular material taking opening (31); the sealing sleeve (4) is mounted on the outer wall of the material taking opening (31); a plug (41) is provided at the center of the sealing sleeve (4) and extends into the material taking opening (31).
2. The Pleurotus geesteranus mother seed storage tube according to claim 1, characterized in that: The material taking opening (31) is eccentrically arranged at the bottom of the material taking nozzle (3), so that the material taking nozzle (3) and a side wall surface of the tube body (1) are connected in a straight line.
3. The Pleurotus geesteranus mother seed storage tube according to claim 1, characterized in that: Limit blocks (11) are evenly arranged on the inner wall of the tube body (1), and the limit blocks (11) are arranged in the middle and upper part of the inner wall of the tube body (1).
4. The Pleurotus geesteranus mother seed storage tube according to claim 1, characterized in that: A limiting groove (42) is provided on the inner wall of the sealing sleeve (4) to cooperate with a limiting ring (32) provided on the outer wall of the material taking nozzle (3).