Portable antigen and oily adjuvant mixing and emulsifying device

Abstract

The portable mixing and emulsifying device for the antigen and the oily adjuvant comprises a mixing pipe, two ends of the mixing pipe are communicated and are respectively provided with a push rod, the opposite ends of the two push rods are provided with pistons, and the pistons have the freedom degree of reciprocating translation in the axial direction of the mixing pipe; the emulsifying flow divider is arranged between the two pistons, divides the inner cavity of the mixing pipe into two pipe cavities, and is provided with a plurality of flow dividing holes communicated with the two pipe cavities; the mixing pipe is connected with a connecting valve exposed out of the outer wall of the mixing pipe, and the connecting valve is communicated with a pipe cavity of the mixing pipe. In this way, the liquid is divided into a plurality of tiny trickles through the flow dividing holes, the emulsification effect is greatly improved, and the resistance encountered when the viscous liquid is emulsified is also conveniently reduced.

Description

Technical Field

[0001] The present application relates to the field of biotechnology, and in particular to a portable antigen and oily adjuvant mixing and emulsification device. Background Art

[0002] In the field of biological products, especially the vaccine industry, the preparation of animal antibody serum has always been an important part of the research and testing process. When immunizing animals, in order to enhance the immunogenicity of the antigen and improve the titer of the immune response, the antigen is usually mixed and emulsified with an oily adjuvant (such as Freund's complete adjuvant or Freund's incomplete adjuvant). The antigen liquid is dispersed in the oily adjuvant to form a stable oil-water mixture. After this mixture is injected into the animal, it can significantly enhance the animal's specific immune response to the antigen and prolong the duration of immunity.

[0003] At present, the emulsification methods used by various biological units mainly include grinding, ultrasonic method and syringe mixing method, but they all have their own disadvantages. For example, the grinding method is suitable for preparing a large number of adjuvant antigens, but a large amount of emulsion adheres to the wall of the mortar, resulting in a large loss of antigens. The ultrasonic method requires strict control of the ultrasonic frequency and time, and ultrasound easily excites some free radicals, causing unknown damage to the antigen. In contrast, the syringe mixing method has the advantages of high portability, less antigen loss for the preparation of a small amount of antigen emulsion, easier to achieve aseptic operation and simple operation.

[0004] The Chinese utility model patent with the announcement number "CN204469576U" discloses an "emulsification device". The emulsification device includes two syringes and a connecting tube, and the two ends of the connecting tube are respectively sleeved on the nipple parts of the two syringes. A structure is adopted in which both ends of the connecting tube are connected to the syringe, and the nipple part of the syringe is lengthened to facilitate close connection with the connecting tube; the whole structure is simple, easy to operate, easy to make, and low cost. As an existing syringe mixing method, there are also technical problems to be solved. For example, since this type of emulsification process only relies on a small tube connecting two syringes to each other, allowing the antigen and the adjuvant to repeatedly pass through the small tube to form an emulsification, it is easy to cause the emulsification to be incomplete. In addition, some antigens and adjuvants are too viscous to be pushed, or when the push rod is pushed, due to the high pressure in the tube, leakage is likely to occur at the connection port, resulting in problems such as antigen loss and contamination. Utility Model Content

[0005] This application is made in view of the above-mentioned state of the prior art. The purpose of this application is to provide a portable antigen and oily adjuvant mixing and emulsification device.

[0006] The technical solution adopted by the present application to solve its technical problems is: a portable antigen and oily adjuvant mixing and emulsification device, comprising: a mixing tube, both ends of which are connected and each is provided with a push rod, the facing ends of the two push rods are provided with pistons, and the pistons have the freedom of reciprocating translation along the axial direction of the mixing tube; an emulsification diverter, which is arranged between the two pistons, the emulsification diverter divides the inner cavity of the mixing tube into two cavities, and the emulsification diverter has a plurality of diversion holes connecting the two cavities; the mixing tube is connected to a connecting valve exposed from the outer wall of the mixing tube, and the connecting valve is connected to the lumen of the mixing tube.

[0007] As a further improvement of the present application, the number of the diversion holes is dozens, and / or the connecting valve is connected to the emulsification diverter and communicates with at least part of the diversion holes.

[0008] As a further improvement of the present application, the inner diameter of the diverter hole is 0.2 mm to 2 mm, the axial length of the diverter hole is 50 mm to 150 mm, and / or the diameter of the inner cavity of the connecting valve is 4 mm to 6 mm; the axis of the diverter hole is parallel to the axis of the mixing tube, and the axis of the connecting valve extends radially along the mixing tube.

[0009] As a further improvement of the present application, the sum of the cross-sectional areas of the diversion holes on the emulsification diverter is larger than the cross-sectional area of ​​the inner cavity of the connecting valve.

[0010] As a further improvement of the present application, the shaft end of the connecting valve is detachably equipped with a sealing cover, and the inner cavity of the connecting valve is used for inserting the nipple of the syringe.

[0011] As a further improvement of the present application, the sealing cover has an internal thread, the connecting valve has an external thread, and the sealing cover and the connecting valve are detachably assembled via the thread.

[0012] As a further improvement of the present application, the sealing cover is connected to the connecting valve via an anti-slip band, both ends of the anti-slip band are provided with annular buckles, the sealing cover and the connecting valve respectively pass through the annular buckles at both ends of the anti-slip band, and the outer walls of the sealing cover and the connecting valve are provided with circumferential grooves movably assembled with the annular buckles.

[0013] As a further improvement of the present application, one side of the sealing cover has an inner convex portion; when the sealing cover is assembled with the connecting valve, the inner convex portion extends into the inner cavity of the connecting valve and occupies at least part of the inner cavity space of the connecting valve.

[0014] As a further improvement of the present application, the inner walls at both ends of the mixing tube are provided with inner ribs extending radially inward, the push rod is provided with a slot step, and the push rod has the freedom of rotation around its own axis; when the push rod rotates and axially limits the slot step and the inner rib, the piston of the push rod comes into contact with the emulsifier diverter.

[0015] As a further improvement of the present application, the mixing tube, the push rod, the emulsifying diverter, the connecting valve, the sealing cover and the anti-slip belt are all made of plastic materials, and the hardness of each of the mixing tube, the push rod, the emulsifying diverter, the connecting valve and the sealing cover is greater than the hardness of the anti-slip belt; the piston is made of rubber or silicone.

[0016] This portable antigen and oily adjuvant mixed emulsification device is equipped with an emulsification diverter in the middle of the mixing tube. When the liquid is emulsified, the liquid is squeezed through a number of diverter holes by repeated pressing, and is thus divided into a number of tiny streams. First, the chances of antigen and adjuvant interaction and mixing are greatly increased, and a more uniform and fine oil-water complex can be formed, which greatly improves the effect and completeness of the emulsification. Secondly, since there are more than one diverter hole, the total area of ​​the cross section of the multiple diverter holes will be larger. Compared with the emulsification form of a single small tube in the prior art, this device is convenient for reducing the resistance encountered when the viscous liquid is emulsified. Finally, when the emulsification is completed and waiting for transfer, the connecting valve connected to the diverter hole can be used to transfer to the outside, such as a syringe. This design of an emulsification diverter built into the overall closed mixing tube also greatly improves the ability to prevent pollution and leakage when the antigen and adjuvant interact. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the technical solutions in the embodiments, the drawings of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0018] Figure 1 It is a front view according to an embodiment of the present application;

[0019] Figure 2 This is a schematic diagram of the docking of an emulsifying diverter and a syringe according to one embodiment of the present application;

[0020] Figure 3 A partial perspective view of a mixing tube according to an embodiment of the present application;

[0021] Figure 4 is a perspective view of a push rod according to one embodiment of the present application;

[0022] Figure 5 is a stereoscopic view of an emulsification diverter according to one embodiment of the present application;

[0023] Figure 6 It is a three-dimensional view of a longitudinal section of an emulsification diverter according to one embodiment of the present application;

[0024] Figure 7 This is a schematic diagram of the operation of the emulsification stage according to one embodiment of the present application;

[0025] Figure 8 It is a schematic diagram of the operation of the transfer stage according to one embodiment of the present application;

[0026] Fig. 9 (a) is a schematic diagram of a first relative position between a push rod and an inner rib plate according to an embodiment of the present application;

[0027] Fig. 9 (b) is a schematic diagram of a second relative position between the push rod and the inner rib plate according to an embodiment of the present application;

[0028] Fig. 9 (c) is a schematic diagram of a third relative position between the push rod and the inner rib plate according to an embodiment of the present application;

[0029] Fig. 9 (d) is a schematic diagram of a third relative position between the push rod and the inner rib according to an embodiment of the present application.

[0030] Description of Reference Numerals

[0031] 1-mixing tube; 2, 3-lumen; 4, 5-push rod; 6-emulsification diverter; 7-connecting valve; 8-sealing cover; 9, 10-piston; 11, 12-slot steps; 13, 14-push cap; 15, 16-outer wing plate; 17, 18-inner rib plate; 19-anti-slip belt; 20-inner convex part; 21-diverter hole; 22-collecting cavity; 23-circumferential groove; 24-syringe; 25-nipple. DETAILED DESCRIPTION

[0032] The portable antigen and oily adjuvant mixing and emulsification device of the present application is further described in detail below in conjunction with specific embodiments.

[0033] like Figure 1 As shown, in an embodiment of the utility model, the portable antigen adjuvant mixing emulsifier may include a mixing tube 1, in which an emulsification diverter 6 is provided in the middle position of the inner cavity of the mixing tube 1, and the emulsification diverter 6 divides the inner cavity of the mixing tube 1 into two left-right symmetrical chambers, namely, cavities 2 and 3, and the inner walls of the cavities 2 and 3 are respectively movably equipped with pistons 9 and 10, and the pistons 9 and 10 are fixedly connected to push rods 4 and 5.

[0034] The outer wall of the mixing tube 1 is marked with scale bars, and the outer walls at both ends of the mixing tube 1 are provided with radially extending outer wing plates 15 and 16, and the inner walls at both ends of the mixing tube 1 are provided with radially extending inner rib plates 17 and 18 (see Figure 3 ), the outer protruding parts of the push rods 4 and 5 are slot steps 11 and 12, and the ends of the push rods 4 and 5 extending out of the mixing tube 1 are fixedly connected with push caps 13 and 14. The outer wing plates 15 and 16 and the push caps 13 and 14 are convenient for the user to contact and apply force.

[0035] See also Figure 2 , Figure 5 , Figure 6 The emulsifying diverter 6 has a plurality of, for example, dozens of, diverter holes 21 that are connected. The emulsifying diverter 6 has a hollow connecting valve 7 that extends out of the outer wall of the mixing tube 1 and is sealed and connected to the outer wall of the mixing tube 1. The connecting valve 7 extends into the inner cavity of the emulsifying diverter 6 and is connected to at least part of the diverter holes 21.

[0036] The inner diameter of the diverter hole 21 may be, for example, 0.2-2 mm, preferably 1 mm. The axial length of the diverter hole 21 may be, for example, 50-150 mm, preferably 100 mm. The inner diameter of the connecting valve 7 may be, for example, 4-6 mm.

[0037] The beneficial effect of the above embodiment is that the emulsification diverter 6 of the portable antigen and oily adjuvant mixed emulsification device contains a plurality of diverter holes 21, which ensures that the total cross-sectional area of ​​the diverter holes 21 is significantly larger than the single-hole nipple 25 of the traditional syringe 24 (see Figure 8 ) is preferably more than 3 times the cross-sectional area of ​​the single-hole nipple 25, and more preferably more than 5 times. The total cross-sectional area of ​​the diverter hole 21 can be more than 1 / 10 of the total cross-sectional area of ​​the lumen of the mixing tube 1, and preferably more than 1 / 5. In this way, under the same pressure on the liquid per unit time, the liquid circulation volume can be significantly increased and the circulation efficiency can be improved. Reducing the resistance generated during the emulsification of viscous liquids can effectively reduce the thrust that the operator needs to apply to the push rods 4 and 5, and reduce the difficulty of operation. The user only needs to push the push rod repeatedly dozens of times to complete the good emulsification of the antigen and adjuvant. The overall time-saving, labor-saving and efficient.

[0038] In other embodiments, the inner diameters of the diverter holes 21 are equal, and the diverter holes 21 are arranged in a circular array or a rectangular array around the axis of the emulsification diverter 6 itself.

[0039] In other embodiments, the emulsification splitter 6 has an inner cavity, i.e. Figure 6 The collecting chamber 22 shown has a diameter greater than the distance between adjacent diverting holes 21. The bottom of the collecting chamber 22 is located at the centroid of the emulsifying diverter 6.

[0040] In other embodiments, the shaft end of the connecting valve 7 is detachably equipped with a sealing cap 8. The connecting valve 7 with the sealing cap 8 removed can be inserted into a disposable sterile syringe 24 on the market and fits well with the nipple 25 of the syringe 24. Figure 1 The figure shows the state where the sealing cover 8 is connected to the connecting valve 7. After the sealing cover 8 and the connecting valve 7 cooperate, the connection with the outside is closed.

[0041] The beneficial effect of adopting the above embodiment is that during the emulsification process, the sealing cover can provide a closed and leak-proof emulsification environment.

[0042] See also Figure 2 , Figure 6 In other embodiments, the sealing cover 8 is designed to be inwardly convex, that is, it has an inwardly convex portion 20. Figure 2 The figure shows the state of the inner convex part 20 when the sealing cover 8 is opened. Figure 6 The figure shows the state of the inner convex part 20 when the sealing cover 8 is closed. Figure 2 The arrow in the figure indicates that after the sealing cover 8 is opened, the syringe 24 can be docked with the connecting valve 7 in the next step.

[0043] The beneficial effect of adopting the above embodiment is that because the connecting valve 7 connects the inside and outside of the mixing tube 1, the exposed connecting valve 7 naturally has an inner cavity, and the antigens and antibodies therein are equivalent to being in a dead angle, forming an invalid diversion space, in order to avoid the antigens and antibodies in this area not being fully emulsified. The inner convex part 20 is used to fill part of the space of the collecting cavity 22, squeezing out the antigens and antibodies that may have accumulated in this dead angle, thereby ensuring that all antigens and antibodies are fully emulsified.

[0044] like Figure 6 As shown, the connecting valve 7 protrudes from the outer wall of the mixing tube 1 and is provided with an external thread, and is tightly assembled with the sealing cover 8 with a thread on the inner wall in a rotating manner.

[0045] In other embodiments, Figure 1 , Figure 2 , Figure 7 As shown, the connecting valve 7 is connected to the sealing cover 8 via a flexible anti-slip belt 19, and one end of the anti-slip belt 19 is connected to the top of the sealing cover 8 via a ring buckle. Figure 5 As shown, a circumferential groove 23 is provided at the top of the sealing cover 8, and the circumferential groove 23 and the annular buckle do not affect the free rotation of the sealing cover 8. The other end of the anti-slip belt 19 is also connected to the connecting valve 7 through the annular buckle, and the outer wall of the connecting valve 7 also has a circumferential groove 23 that is movably assembled with the annular buckle. For the convenience of expression, Figure 5 The anti-falling belt 19 is hidden in the middle. Figure 6 is Figure 5 A three-dimensional image of the remaining half after longitudinal section.

[0046] The beneficial effect of adopting the above embodiment is that the anti-slip belt 19 can effectively prevent the sealing cover 8 from being lost or contaminated after opening.

[0047] In other embodiments, Figure 1 As shown, the lumen 2, the push rod 4, the piston 9, the slot step 11, the push cap 13, and the outer wing plate 15 are located on the same side of the emulsification diverter, and the lumen 3, the push rod 5, the piston 10, the slot step 12, the push cap 14, and the outer wing plate 16 are located on the other side of the emulsification diverter. Figure 3 As shown, the inner ribs 17 and 18 make the openings at both ends of the mixing tube 1 non-circular. The inner ribs 17 and 18 can be formed only at the ends of the mixing tube 1 close to the outer wing plates 15 and 16, without affecting the internal shape of other positions of the mixing tube 1. The cross section of the inner wall of the mixing tube 1 except the two ends can be circular. The shape of the inner ribs 17 and 18 is arched. Figure 4 As shown, the cross-sectional profile of the push rods 4, 5 is a cross. The thickness of the push rods 4, 5 between the slot steps 11, 12 and the push caps 13, 14 is smaller than the thickness of the push rods 4, 5 between the slot steps 11, 12 and the pistons 9, 10.

[0048] The beneficial effect of adopting the above embodiment is that the design of the slot step 11 and the inner ribs 17, 18 provides a locking position for the push rods 4, 5. After the push rods 4, 5 are pushed inward to the limit position, the push rods 4, 5 are rotated to a certain angle, and the slot steps 11, 12 are rotated to the inner side of the inner ribs 17, 18, thereby limiting the freedom of the push rods 4, 5 to extend axially. This locking design can facilitate the transfer of emulsified liquid, that is, one of the two push rods 4, 5 is allowed to move and the other is locked, which is convenient for personnel to operate with their hands, otherwise it is difficult for personnel to operate with both hands.

[0049] In order to demonstrate the entire locking principle, Fig. 9 As shown, Fig. 9 (a) is the starting stage, in which the slot steps 11 and 12 are still outside the mixing tube 1. Fig. 9 Based on (a), push the push rods 4 and 5 forward to obtain Fig. 9 The position state corresponding to (b) is Fig. 9 The slot steps 11 and 12 in (b) have successfully entered the mixing tube 1, that is, the slot steps 11 and 12 have moved from one side of the inner ribs 17 and 18 to the other side. Fig. 9 Based on (b), the push rods 4 and 5 are rotated, generally about 90°, to obtain Fig. 9 (c), Fig. 9 The slot steps 11 and 12 in (c) are in direct contact with one side of the inner ribs 17 and 18. At this time, the inner ribs 17 and 18 play a locking and limiting role for the slot steps 11 and 12. Fig. 9 (d) shows from another perspective Fig. 9 (c) corresponds to the state. In order to show the action principle more clearly, Fig. 9 The mixing tube 1 in the figure adopts the fracture drawing method, and in order to more clearly show the positional relationship between the slot steps 11 and 12, compared with Fig. 9 (a) to Fig. 9 (c), Fig. 9 (d) shows the outlines of the push rods 4, 5 and the groove steps 11, 12 that are visually blocked by the mixing tube 1.

[0050] In other embodiments, during the processing stage, the inner ribs 17 and 18 are independent components, which are fixed to the mixing tube 1 by welding or other methods, so as to facilitate the pistons 9 and 10 to enter the cavities 2 and 3.

[0051] In other embodiments, the mixing tube 1, push rods 4, 5, push caps 13, 14, emulsifying diverter 6, connecting valve 7, and sealing cover 8 are all made of hard plastic, the anti-slip belt 19 is made of soft plastic, and the pistons 9, 10 are made of rubber or silicone.

[0052] The beneficial effects of adopting the above embodiment are: many parts of the device are similar to the main structure of the existing syringe 24, and the device is easy to modify, which reduces the manufacturing cost. In addition, the pistons 9 and 10 made of rubber or silicone have good sealing properties. The anti-slip belt 19 is relatively softer and plays a role similar to a flexible hinge, so that the sealing cover 8 can be smoothly removed when necessary to make room for the space near the shaft end of the connecting valve 7.

[0053] The working steps of the mixing emulsifier of the embodiment of the utility model may include a preparation stage, an emulsification stage, and a transfer stage.

[0054] The initial state of the portable antigen adjuvant mixing emulsifier before use is that the sealing cover 8 is screwed on the connecting valve, the pistons 9 and 10 in the mixing tube 1 are tightly attached to the emulsifying diverter 6, and the groove steps 11 and 12 are respectively against the inner ribs 17 and 18, so that the push rods 4 and 5 are in an immobile state.

[0055] In the preparation stage, when using the device for antigen-antibody emulsification, first pinch the push caps 13 and 14 with your fingers, rotate the push rods 4 and 5 counterclockwise or clockwise, for example, by 90°, and rotate the pistons 9 and 10 together by about 90°, so that the slot steps 11 and 12 are out of contact with the inner ribs 17 and 18, releasing the mobility of the push rods 4 and 5. Then Figure 2 As shown, the sealing cover 8 is rotated to open, the nipple 25 of the syringe 24 containing the antigen or adjuvant is tightly inserted into the connecting valve 7 and the solution is injected into the mixing tube 1. The solution naturally flows into the lumen 2 and the lumen 3. After the addition of samples is completed, the syringe 24 is removed and the sealing cover 8 is screwed onto the connecting valve 7 again.

[0056] In the emulsification stage, one operation step is as follows Figure 7 As shown, use the palms of both hands to respectively press against the push caps 13 and 14, and hook the fingers on the outer wing plates 15 and 16. Figure 7 As shown by the reciprocating arrows in the figure. Push the push caps 13 and 14 back and forth repeatedly with both hands. The push caps 13 and 14 move synchronously in the same direction. The antigen and adjuvant mixed solution is repeatedly and rapidly contacted through the emulsification diverter 6, and rapidly transferred between the lumen 2 and the lumen 3, and emulsified efficiently. The emulsification process can be completed by repeating this process more than ten to dozens of times. The solution in the mixing tube 1 gradually changes from a transparent liquid with visible oil-water separation to a uniform and delicate milky white liquid. After the emulsification is completed, the mixed solution is first completely pushed into the lumen 2 or lumen 3.

[0057] During the transition phase, Figure 7 After the emulsification stage shown, enter Figure 8 The transfer stage shown. One operation step is to push the liquid into the lumen 3, that is, to continuously push the push cap 13 until the piston 9 contacts the emulsification diverter 6, at which time the emulsified liquid is squeezed into the lumen 3. Then the push cap 13 is rotated to a certain angle so that the groove step 11 on the push rod 4 abuts against the inner rib 17, thereby locking the axial mobility of the push rod 4. Next, remove the sealing cap 8, and then tightly insert the nipple 25 of the syringe 24 for inoculating animals into the connecting valve 7. Figure 8 As shown, one hand along Figure 8 As shown by the one-way arrow in the figure, another push cap 14 is pressed to allow the emulsified liquid to slowly enter the syringe 2 from the lumen 3 through the collection chamber 22 until it is completely transferred. Finally, the syringe 24 is unplugged and the needle is connected to the animal for inoculation. Of course, Figure 8 In the embodiment, the push cap 13 is locked and the push cap 14 is allowed to move. In actual use, the push cap 14 can also be locked and the push cap 13 can be allowed to move.

[0058] This device belongs to the category of syringe mixing method, so naturally there is no problem of huge antigen loss in grinding method and unknown damage to antigen in ultrasonic method. There are other antigen adjuvant emulsification schemes that require electricity, the weight and volume of the emulsification device are too large to be carried, it is difficult to sterilize, it is difficult to clean, and so on. At the same time, this device is an improvement on the syringe mixing method. By using a large number of diversion holes 21, the phenomenon of incomplete emulsification caused by the single diameter of the connection at the traditional syringe mixing method is overcome, or the phenomenon that individual antigens or adjuvants cannot be promoted to mix due to viscosity, or the phenomenon that the connection is loose and leaks under high pressure, causing antigen loss and contamination.

[0059] The device is small in size and can be used in disposable sterile packaging, which is convenient to carry and can be used immediately after disassembly. It solves the problems of other antigen adjuvant emulsification solutions, such as the need for electricity, large weight and volume, and difficulty in carrying, sterilization, and cleaning.

[0060] The above embodiments are only for illustrating the technical concept and features of the present application, and their purpose is to enable people familiar with this technology to understand the content of the present application and implement it. They cannot be used to limit the scope of protection of the present application. All equivalent changes or modifications made according to the spirit of the present application should be included in the scope of protection of the present application.

Claims

1. A portable antigen and oily adjuvant mixing emulsification device, characterized in that: include: A mixing tube, both ends of which are penetrated and each is provided with a push rod, and the facing ends of the two push rods are provided with a piston, and the piston has the freedom of reciprocating translation along the axial direction of the mixing tube; An emulsifying diverter is arranged between the two pistons, and the emulsifying diverter divides the inner cavity of the mixing tube into two cavities. The emulsifying diverter has a plurality of diverting holes connecting the two cavities. The mixing tube is connected to a connecting valve exposed from the outer wall of the mixing tube, and the connecting valve is connected to the cavity of the mixing tube.

2. The portable antigen and oily adjuvant mixing and emulsification device according to claim 1, characterized in that: The number of the diversion holes is dozens, and / or The connecting valve is connected to the emulsification diverter and communicates with at least part of the diverting holes.

3. The portable antigen and oily adjuvant mixing and emulsification device according to claim 1, characterized in that: The inner diameter of the diverter hole is 0.2 mm to 2 mm, the axial length of the diverter hole is 50 mm to 150 mm, and / or The diameter of the inner cavity of the connecting valve is 4 mm to 6 mm; the axis of the diverter hole is parallel to the axis of the mixing tube, and the axis of the connecting valve extends along the radial direction of the mixing tube.

4. The portable antigen and oily adjuvant mixing and emulsification device according to claim 1, characterized in that: The sum of the cross-sectional areas of the diverter holes on the emulsifying diverter is greater than the cross-sectional area of ​​the inner cavity of the connecting valve.

5. The portable antigen and oily adjuvant mixing and emulsification device according to claim 1, characterized in that: The shaft end of the connecting valve is detachably equipped with a sealing cover, and the nipple of the syringe is inserted into the inner cavity of the connecting valve.

6. The portable antigen and oily adjuvant mixing and emulsification device according to claim 5, characterized in that: The sealing cover has an internal thread, the connecting valve has an external thread, and the sealing cover and the connecting valve are detachably assembled through the thread.

7. The portable antigen and oily adjuvant mixing and emulsification device according to claim 5, characterized in that: The sealing cover and the connecting valve are connected via an anti-slip band, both ends of the anti-slip band are provided with annular buckles, the sealing cover and the connecting valve respectively pass through the annular buckles at both ends of the anti-slip band, and the outer walls of the sealing cover and the connecting valve are provided with circumferential grooves movably assembled with the annular buckles.

8. The portable antigen and oily adjuvant mixing and emulsification device according to claim 5, characterized in that: One side of the sealing cover is provided with an inner convex portion; when the sealing cover is assembled with the connecting valve, the inner convex portion extends into the inner cavity of the connecting valve and occupies at least part of the inner cavity space of the connecting valve.

9. The portable antigen and oily adjuvant mixing and emulsification device according to claim 7, characterized in that: The inner walls at both ends of the mixing tube are provided with inner ribs extending radially inward, the push rod is provided with a slot step, and the push rod has the freedom of rotation around its own axis; when the push rod rotates and the slot step and the inner rib are axially limited, the piston of the push rod comes into contact with the emulsifying diverter.

10. The portable antigen and oily adjuvant mixing and emulsification device according to claim 9, characterized in that: The mixing tube, the push rod, the emulsifying diverter, the connecting valve, the sealing cover, and the anti-slip belt are all made of plastic materials, and the hardness of each of the mixing tube, the push rod, the emulsifying diverter, the connecting valve, and the sealing cover is greater than the hardness of the anti-slip belt; the piston is made of rubber or silicone.

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