Stem cell storage device

By designing the technology of motor-driven belt rotation and exhaust frame up and down in stem cell storage equipment, the circulating transmission and uniform distribution of liquid nitrogen are achieved, solving the problem of damage to cells by uneven distribution of liquid nitrogen, ensuring cell survival and functional retention, and improving system maintenance efficiency.

CN222548446UActive Publication Date: 2025-03-04HENAN INTECEL BIOTECHNOLOGY CO LTD
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Patent Information

Application Number
CN202420082687.7
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-01-13
Publication Date
2025-03-04
Estimated Expiration
2034-01-13

AI Technical Summary

Technical Problem

In existing stem cell storage devices, the uneven distribution and oscillation of liquid nitrogen adversely affects cell survival and mass, resulting in the possibility of cell death or loss of function.

Method used

A stem cell storage device is designed to drive the belt to rotate through a motor, combining the up and down movement of the exhaust frame and connecting block to achieve cyclic transmission and uniform distribution of liquid nitrogen to ensure the constant temperature state of the cells under low temperature conditions.

Benefits of technology

Through the uniform distribution and circulating transmission of liquid nitrogen, the damage caused by cold inequality is avoided, the survival and functional retention of cells is ensured, and the maintenance efficiency of the system is improved.

✦ Generated by Eureka AI based on patent content.

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Abstract

The utility model provides a stem cell storage device, which relates to the technical field of stem cell storage equipment and comprises an adjusting mechanism, a sealing mechanism is arranged on one side of the adjusting mechanism, the adjusting mechanism comprises a storage box, and a side box is arranged on one side of the storage box. According to the utility model, the motor is started, the belt is rotated, the connecting block moves upwards, the exhaust frame and the connecting block are integrated, the exhaust frame continuously discharges liquid nitrogen from cell culture dishes distributed at different heights in the storage box when moving upwards, and when moving upwards to a certain degree, the liquid nitrogen in the cell culture dishes is discharged by a manual mode. And the motor is operated to rotate reversely to enable the exhaust frame to move downwards, so that circulating liquid nitrogen transmission is realized, uniform distribution of the liquid nitrogen in the cell storage process can be ensured, and damage caused by non-uniform cooling of cells is avoided.
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Description

Technical Field

[0001] The utility model relates to the technical field of stem cell storage equipment, in particular to a stem cell storage device. Background Art

[0002] Cell banking refers to the storage of living cells or tissues at low temperatures to maintain their vitality and function for a long time. This storage method is often used to preserve plant, animal and human cells for research, medical treatment or biotechnology applications.

[0003] In the existing technology, when storing cells, they need to be placed in culture dishes. During storage, since the box can accommodate multiple culture dishes, the distribution of the culture dishes in the box is different. However, during storage, cells can only survive under low temperature conditions. Since the liquid nitrogen exhaust port may be at the top of the box, the culture dishes at the bottom may not reach the appropriate temperature, which may cause cell death or loss of function, thereby affecting their subsequent applications. At the same time, because the liquid nitrogen cannot be evenly distributed, the position of the cells is subjected to different degrees of cooling and vibration. This airflow effect may have an adverse effect on cell survival and quality. Utility Model Content

[0004] The purpose of the utility model is to solve the shortcomings of the prior art and provide a stem cell storage device.

[0005] In order to achieve the above-mentioned purpose, the utility model adopts the following technical scheme: a stem cell storage device, comprising: an adjustment mechanism, a sealing mechanism is arranged on one side of the adjustment mechanism, the adjustment mechanism comprises a storage box, a side box is arranged on one side of the storage box, a motor is arranged on one side of the side box, a roller is arranged on the output end of the motor, a belt is arranged on the outside of the roller, a connecting block is arranged on the outside of the belt, an exhaust frame is arranged at one end of the connecting block, two drainage tubes are arranged on one side of the exhaust frame, two locking rings are arranged on one end of the drainage tubes, two connecting rings are arranged on one side of the locking ring, and a liquid nitrogen tank is arranged at one end of each connecting ring.

[0006] As a preferred embodiment, the sealing mechanism includes a sealing strip, one side of which is fixedly connected to one side of the side box, a guide tube is connected through the interior of the side box, one end of the guide tube is fixedly connected to one side of the storage box, and the interior of the guide tube is threadedly connected to the outer side of the bolt.

[0007] As a preferred embodiment, one side of the storage box is in contact with one side of the side box, the bottom end of the side of the side box is fixedly connected to the outer surface of the motor, and the output end of the motor is fixedly connected to one end of the roller.

[0008] As a preferred embodiment, the outer side of the roller is drivingly connected to the inner surface of the belt, and the outer side of the belt is fixedly connected to the inside of the connecting block.

[0009] As a preferred implementation, two sides of the connection block are slidably connected to the inner wall of the fixing plate, and the upper and lower ends of the fixing plate are fixedly connected to the inner wall of the side box.

[0010] As a preferred embodiment, one end of the connecting block is fixedly connected to one side of the exhaust frame, one side of the exhaust frame is fixedly connected to one end of the drainage tube, and the other end of the drainage tube is movably connected to the inner surface of the locking ring.

[0011] As a preferred implementation, the inner ring of the locking ring and the outer ring of the connecting ring are engaged with each other, and one end of the connecting ring is fixedly connected to the outer side of the liquid nitrogen tank.

[0012] As a preferred implementation, the outer side of the liquid nitrogen tank is in contact with one end of the side surface of the side box.

[0013] Compared with the prior art, the advantages and positive effects of the utility model are:

[0014] 1. Start the motor and rotate the belt, and the connecting block will move up. Since the exhaust frame and the connecting block are integrated, the exhaust frame will continuously discharge liquid nitrogen from the cell culture dishes at different heights in the storage box when it moves up. When it moves up to a certain extent, the motor is reversed manually to move the exhaust frame down, thereby realizing the circulation of liquid nitrogen transmission. This can ensure the uniform distribution of liquid nitrogen during cell storage and avoid damage to cells due to uneven cooling.

[0015] 2. The side box is released by removing the bolts, and then the side box is moved to one side so that it is no longer combined with the storage box. The main purpose is that since there are many parts in the side box, this method can be used to regularly maintain the parts in the side box, avoiding interference with the operation of the entire system and improving the accuracy and efficiency of maintenance. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a schematic structural diagram of a stem cell storage device provided by the utility model.

[0017] Figure 2 This is a schematic diagram of the component cross-sectional structure of a stem cell storage device provided by the utility model.

[0018] Figure 3 This is a schematic diagram of the side structure of a component of a stem cell storage device provided by the utility model.

[0019] Figure 4This is a schematic diagram of the component disassembly structure of a stem cell storage device provided by the utility model.

[0020] Figure 5 This is a schematic diagram of the enlarged structure of components of a stem cell storage device provided by the utility model.

[0021] Legend:

[0022] 1. Adjustment mechanism; 11. Storage box; 12. Side box; 13. Motor; 14. Roller; 15. Belt; 16. Connecting block; 17. Exhaust frame; 18. Fixing plate; 19. Drainage tube; 110. Locking ring; 111. Connecting ring; 112. Liquid nitrogen tank

[0023] 2. Sealing mechanism; 21. Sealing strip; 22. Guide tube; 23. Bolt. DETAILED DESCRIPTION

[0024] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model. Example

[0025] like Figure 1 - Figure 5As shown, the utility model provides a technical solution: a stem cell storage device, comprising: an adjustment mechanism 1, a sealing mechanism 2 is arranged on one side of the adjustment mechanism 1, the adjustment mechanism 1 comprises a storage box 11, a side box 12 is arranged on one side of the storage box 11, a motor 13 is arranged on one side of the side box 12, a roller 14 is arranged on the output end of the motor 13, a belt 15 is arranged on the outside of the roller 14, a connecting block 16 is arranged on the outside of the belt 15, an exhaust frame 17 is arranged on one end of the connecting block 16, two drainage tubes 19 are arranged on one side of the exhaust frame 17, two locking rings 110 are arranged on one end of the drainage tubes 19, two connecting rings 111 are arranged on one side of the locking ring 110, and a liquid nitrogen tank 112 is arranged on one end of the connecting ring 111, one side of the storage box 11 and one side of the side box 12 are in contact with each other, and the side box 1 2 is fixedly connected to the outer surface of the motor 13, the output end of the motor 13 is fixedly connected to one end of the roller 14, the outer side of the roller 14 is drivingly connected to the inner surface of the belt 15, the outer side of the belt 15 is fixedly connected to the inside of the connecting block 16, both sides of the connecting block 16 are slidably connected to the inner wall of the fixing plate 18, the upper and lower ends of the fixing plate 18 are fixedly connected to the inner wall of the side box 12, one end of the connecting block 16 is fixedly connected to one side of the exhaust frame 17, one side of the exhaust frame 17 is fixedly connected to one end of the drainage pipe 19, the other end of the drainage pipe 19 is movably connected to the inner surface of the locking ring 110, the inner ring of the locking ring 110 and the outer ring of the connecting ring 111 are clamped with each other, one end of the connecting ring 111 is fixedly connected to the outer side of the liquid nitrogen tank 112, and the outer side of the liquid nitrogen tank 112 is in contact with one end of the side of the side box 12.

[0026] In this embodiment, in order to allow the cells to better receive the low temperature effect and be evenly distributed during storage, the liquid nitrogen is continuously transmitted to the drainage tube 19 through the liquid nitrogen tank 112. After the drainage tube 19 introduces liquid nitrogen, it will be transmitted to the exhaust frame 17 as soon as possible. At this time, the motor 13 is started to transmit power to the roller 14 and rotate the belt 15. The rotation mainly relies on the power of the motor 13, so it is only necessary to rotate the belt 15 half a circle. As the belt 15 rotates, the connecting block 16 moves up. Since the exhaust frame 17 and the connecting block 16 are connected, the exhaust frame 17 and the connecting block 16 are connected. The block 16 is of an integrated type. Then, when the connection block 16 moves upward, the exhaust frame 17 will also move upward. When moving upward, the exhaust frame 17 will continuously discharge liquid nitrogen from the cell culture dishes distributed at different heights in the storage box 11. When it moves upward to a certain extent, the motor 13 is manually operated to reverse, so that the exhaust frame 17 moves downward, thereby realizing the transmission of circulating liquid nitrogen. This can ensure the uniform distribution of liquid nitrogen during the cell storage process, avoid damage to the cells due to uneven cooling, and maintain a continuous supply of liquid nitrogen to ensure a constant temperature state during the cell storage process.

[0027] Secondly, as the liquid nitrogen tank 112 is used, its energy will be exhausted and it will no longer provide liquid nitrogen. Therefore, when replacing it, it is only necessary to rotate the lock ring 110 of the drainage tube 19 half a circle so that the lock ring 110 no longer buckles the connecting ring 111 on the liquid nitrogen tank 112, and the connecting ring 111 is released, and then the original liquid nitrogen tank 112 can be replaced. In this way, the original liquid nitrogen tank 112 can be easily replaced. This can save time and energy in replacing the liquid nitrogen tank 112 and improve operational efficiency. Example

[0028] like Figure 1 - Figure 4 As shown, the sealing mechanism 2 includes a sealing strip 21, one side of the sealing strip 21 is fixedly connected to one side of the side box 12, a guide tube 22 is connected through the inside of the side box 12, one end of the guide tube 22 is fixedly connected to one side of the storage box 11, and the inside of the guide tube 22 is threadedly connected to the outside of the bolt 23.

[0029] In this embodiment, the bolts 23 are removed to release the side box 12, and then the side box 12 is moved to one side so that it is no longer combined with the storage box 11. The main purpose is that since there are many parts in the side box 12, the parts in the side box 12 can be regularly maintained in this way to avoid interference with the operation of the entire system, thereby improving the accuracy and efficiency of maintenance. In addition, the sealing strip 21 is designed on one side of the side box 12, which can reduce the gap at the connection between the side box 12 and the storage box 11 when the side box 12 is combined with the storage box 11, thereby avoiding excessive loss of liquid nitrogen. Reducing the gap at the connection can reduce the possibility of outside air entering the side box 12 and the storage box 11, thereby helping to maintain a stable low-temperature environment inside the storage box 11.

[0030] Working principle:

[0031] like Figure 1 - Figure 5 As shown, when the utility model is in use, according to the needs of the user, the liquid nitrogen is continuously transmitted to the drainage tube 19 through the liquid nitrogen tank 112, and after the drainage tube 19 introduces the liquid nitrogen, it will be transmitted to the exhaust frame 17 in the first time. At this time, by starting the motor 13 and rotating the belt 15, as the belt 15 rotates, the connecting block 16 will move up. Since the exhaust frame 17 and the connecting block 16 are integrated, the exhaust frame 17 will also move up when the connecting block 16 moves up. When moving up, the exhaust frame 17 will continuously discharge liquid nitrogen from the cell culture dishes distributed at different heights in the storage box 11. When it moves up to a certain extent, the motor 13 is manually reversed to move the exhaust frame 17 downward, thereby realizing the cyclic liquid nitrogen transmission, which can ensure the uniform distribution of liquid nitrogen during the cell storage process.

[0032] By removing the bolts 23, the side box 12 is released, and then the side box 12 is moved to one side so that it is no longer combined with the storage box 11. The main purpose is that since there are many parts in the side box 12, the parts in the side box 12 can be regularly maintained in this way to avoid interference with the operation of the entire system, thereby improving the accuracy and efficiency of maintenance.

[0033] The above description is only a preferred embodiment of the present invention and does not limit the present invention in other forms. Any technician familiar with the profession may use the technical content disclosed above to change or modify it into an equivalent embodiment with equivalent changes and apply it to other fields. However, any simple modification, equivalent change and modification made to the above embodiment based on the technical essence of the present invention without departing from the content of the technical solution of the present invention still falls within the protection scope of the technical solution of the present invention.

Claims

1. A stem cell storage device, characterized in that: include: An adjusting mechanism (1), wherein a sealing mechanism (2) is provided on one side of the adjusting mechanism (1), wherein the adjusting mechanism (1) comprises a storage box (11), wherein a side box (12) is provided on one side of the storage box (11), wherein a motor (13) is provided on one side of the side box (12), wherein a roller (14) is provided at the output end of the motor (13), wherein a belt (15) is provided on the outside of the roller (14), wherein a connecting block (16) is provided on the outside of the belt (15), wherein an exhaust frame (17) is provided at one end of the connecting block (16), wherein two drainage pipes (19) are provided on one side of the exhaust frame (17), wherein two locking rings (110) are provided at one end of each of the drainage pipes (19), wherein two connecting rings (111) are provided on one side of each of the locking rings (110), wherein a liquid nitrogen tank (112) is provided at one end of each of the connecting rings (111).

2. A stem cell storage device according to claim 1, characterized in that: The sealing mechanism (2) comprises a sealing strip (21), one side of the sealing strip (21) is fixedly connected to one side of the side box (12), a guide tube (22) is connected through the inside of the side box (12), one end of the guide tube (22) is fixedly connected to one side of the storage box (11), and the inside of the guide tube (22) is threadedly connected to the outside of the bolt (23).

3. A stem cell storage device according to claim 1, characterized in that: One side of the storage box (11) is in contact with one side of the side box (12), the bottom end of the side surface of the side box (12) is fixedly connected to the outer surface of the motor (13), and the output end of the motor (13) is fixedly connected to one end of the rotating roller (14).

4. A stem cell storage device according to claim 1, characterized in that: The outer side of the rotating roller (14) is drivingly connected to the inner surface of the belt (15), and the outer side of the belt (15) is fixedly connected to the inside of the connecting block (16).

5. A stem cell storage device according to claim 1, characterized in that: Both sides of the connection block (16) are slidably connected to the inner wall of the fixing plate (18), and the upper and lower ends of the fixing plate (18) are fixedly connected to the inner wall of the side box (12).

6. A stem cell storage device according to claim 1, characterized in that: One end of the connection block (16) is fixedly connected to one side of the exhaust frame (17), one side of the exhaust frame (17) is fixedly connected to one end of the drainage tube (19), and the other end of the drainage tube (19) is movably connected to the inner surface of the locking ring (110).

7. A stem cell storage device according to claim 1, characterized in that: The inner ring of the locking ring (110) and the outer ring of the connecting ring (111) are mutually clamped, and one end of the connecting ring (111) is fixedly connected to the outer side of the liquid nitrogen tank (112).

8. A stem cell storage device according to claim 1, characterized in that: The outer side of the liquid nitrogen tank (112) contacts one end of the side surface of the side box (12).