High-throughput RCL screening device

Abstract

The utility model relates to the technical field of virus screening, and discloses a high-flux RCL screening device which comprises a cabinet body and a spectrograph, the spectrograph is installed at the lower right corner in the cabinet body, two sets of constant-temperature chambers are transversely arranged on the left side in the cabinet body, and rotating shafts are movably connected between the upper portions and the lower portions of the left front portions in the constant-temperature chambers. A rotating disc is transversely fixed to the left side of the rotating shaft, and six sets of inserting grooves are formed in the rotating disc. According to the high-throughput RCL screening device, a rotating disc is transversely fixed to the left side of a rotating shaft, the rotating disc connected with the rotating shaft is shifted from the left side of a constant-temperature cavity, bacterial liquid to be cultured is poured into a bacterial liquid bottle, a bottle cap is closed, then the bacterial liquid bottle and the bacterial liquid bottle are placed into an insertion groove together and then screwed into the constant-temperature cavity, a ventilator is started, culture is conducted at the constant temperature, and the rotating disc is rotated out when the bacterial liquid is taken out; and the left side of the constant-temperature chamber is blocked by the rotating baffle plate, so that the problem of lack of culture space is solved.

Description

Technical Field

[0001] The utility model relates to the technical field of virus screening, in particular to a high-throughput RCL screening device. Background Art

[0002] RCL is a replicating lentivirus that has the ability to replicate and will produce mutations in the vector. This virus can be used in experimental research with drug cells. After making multiple groups of comparative controls, the survival of the RCL virus can be checked to test the function of the drug cells. Therefore, in the process of drug development, the screening of RCL is crucial.

[0003] Previous high-throughput screening methods often chose to inject the drug cell solution to be selected into the vessel to be cultured, and then inject the RCL virus bacterial solution into this solution. After a period of culture, the survival rate of the bacteria can be detected by a spectrometer. However, this method requires additional culture space, which is not convenient to carry and requires moving a certain distance, and is easily contaminated by external bacteria.

[0004] Now, a new high-throughput RCL screening device is proposed to solve the above problems. Utility Model Content

[0005] The purpose of the utility model is to provide a high-throughput RCL screening device to solve the problem of lack of culture space mentioned in the above background technology.

[0006] In order to achieve the above-mentioned purpose, the utility model provides the following technical solutions: a high-throughput RCL screening device, comprising a cabinet and a spectrometer, a spectrometer is installed at the lower right corner of the cabinet, two groups of constant temperature chambers are horizontally arranged on the left side of the cabinet, and a rotating shaft is movably connected between the upper and lower parts of the left front of the constant temperature chamber, a rotating disk is horizontally fixed on the left side of the rotating shaft, and six groups of slots are arranged inside the rotating disk, bacterial liquid bottles are inserted in the slots, and bottle caps are inserted on the top of the bacterial liquid bottles, a cover plate is horizontally inserted on the top of the rotating disk, six groups of sliding rods are movably inserted under the rotating disk, two groups of ventilation fans are installed at the rear of the constant temperature chamber, and two groups of shielding plates are movably connected to the surface of the left side of the cabinet.

[0007] Preferably, the rotating disk can be rotated horizontally into the constant temperature chamber, and the shielding plate can close the left side of the constant temperature chamber.

[0008] Preferably, the top of the slide bar is attached to the bottom of the bacteria liquid bottle, and the top of the slide bar can slide up and down in the slot.

[0009] Preferably, a circular groove is provided at the upper right corner of the exterior of the cabinet, and light bulbs are installed on both sides of the bottom of the circular groove, movable chambers are provided on both sides of the circular groove, and a plug rod is horizontally inserted between the movable chamber and the circular groove, a card block is fixedly connected to one side of the plug rod, a spring is horizontally fixed between the movable chamber and the plug rod, and a grid line plate is inserted in the circular groove.

[0010] Preferably, the clamping blocks are symmetrically clamped on two sides of the circular groove, and the insertion rod is embedded in the spring.

[0011] Preferably, the insertion rod can be inserted between the active chamber and the circular groove, and the insertion rod can slide horizontally along the inner wall of the active chamber.

[0012] Preferably, a storage groove is horizontally arranged inside the lower left corner of the cabinet, and an operating table is movably inserted in the storage groove, a frame is fixed on the left side of the outside of the cabinet, and a liquid tank is installed on the top of the outside of the frame, a liquid pump is installed on the upper left side of the outside of the liquid tank, a shunt pipe is fixed on the outer wall of the left side of the frame, a spray pipe is fixed on the inner wall of the left side of the frame, and a spray disc is fixed on the right side of the outside of the spray pipe, a liquid pipe is connected between the output end of the liquid pump and the shunt pipe, a ultraviolet lamp is horizontally fixed on the top of the frame, and a glass plate is installed at the back of the frame.

[0013] Preferably, the shunt pipe is connected to the spray pipe, and the liquid pipe is connected between the liquid pump and the shunt pipe.

[0014] Compared with the prior art, the utility model has the following beneficial effects: the high-throughput RCL screening device not only realizes the integration of culture and inoculation, facilitates the calculation of bacterial flora, but also realizes the clean implementation of inoculation operation;

[0015] 1. A rotary disc is fixed horizontally on the left side of the rotating shaft. The rotary disc connected to the rotating shaft is turned on the left side of the constant temperature chamber to pour the bacterial solution to be cultured into the bacterial solution bottle. After closing the bottle cap, the bottle is placed in the slot for storage. Then, the bottle is screwed into the constant temperature chamber and the ventilation fan is started to culture at a constant temperature. When taking out, the rotary disc is also turned out, and then the slide bar is lifted from the bottom to the top to push out the bacterial solution bottle. The left side of the constant temperature chamber is blocked by a rotating shielding plate to prevent the bacteria from being contaminated.

[0016] 2. By inserting a grid line plate in the circular groove, the rod is moved horizontally along the movable chambers on both sides to guide the symmetrical card blocks to slide along the side walls of the circular groove. The coated and grown plates are placed into the circular groove one by one and then the rod is released. Under the action of the spring retraction, the placed plates can be clamped. The grid line plate is placed above the glass plate, and the bulbs on both sides below are turned on to provide light, which is convenient for counting the strains and prevents the plates from being displaced during the counting process.

[0017] 3. By fixing a frame on the left side outside the cabinet, opening the glass plate behind the frame, and pulling out the operating table from the storage slot at the bottom, inoculation, coating, pipetting and other operations can be performed. After starting and ending the operation, the glass plate can be closed, the liquid pump can be started, and the disinfectant liquid can be drawn from the liquid tank to the shunt pipe through the liquid pipe, and then sprayed on the inner wall of the frame through multiple spray discs, and then the ultraviolet light can be started for dozens of minutes of irradiation to eliminate the germs inside the frame and avoid bacterial contamination during operation. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in the present invention. For ordinary technicians in this field, other drawings can also be obtained based on these drawings.

[0019] Figure 1 It is a front view cross-sectional structural schematic diagram of the utility model;

[0020] Figure 2 This is a schematic diagram of the cross-sectional structure of the constant temperature chamber of the utility model;

[0021] Figure 3 This is a schematic diagram of the circular groove structure from top view of the utility model;

[0022] Figure 4 For the utility model Figure 1 Schematic diagram of the enlarged structure of the local section at point A in the middle.

[0023] In the figure: 1. cabinet; 2. spectrometer; 3. constant temperature chamber; 4. movable chamber; 5. round groove; 6. bulb; 7. frame; 8. liquid tank; 9. ultraviolet lamp; 10. liquid pump; 11. liquid pipe; 12. glass plate; 13. shunt pipe; 14. spray pipe; 15. spray plate; 16. rotary plate; 17. operating table; 18. slide bar; 19. storage slot; 20. ventilation fan; 21. rotating shaft; 22. baffle plate; 23. cover plate; 24. bottle cap; 25. slot; 26. bacterial liquid bottle; 27. grid line plate; 28. plug rod; 29. ​​spring; 30. block. DETAILED DESCRIPTION

[0024] In order to enable those skilled in the art to better understand the technical solution of the present invention, the present invention will be further described in detail below in conjunction with the accompanying drawings.

[0025] Example 1: Please refer to Figure 1-4, a high-throughput RCL screening device, comprising a cabinet 1 and a spectrometer 2, wherein the spectrometer 2 is installed at the lower right corner of the cabinet 1, two groups of constant temperature chambers 3 are arranged horizontally on the left side of the cabinet 1, and a rotating shaft 21 is movably connected between the upper and lower parts of the left front of the constant temperature chamber 3, a rotating disk 16 is fixed horizontally on the left side of the rotating shaft 21, and six groups of slots 25 are arranged inside the rotating disk 16, wherein a bacterial liquid bottle 26 is inserted in the slot 25, and a bottle cap 24 is inserted on the top of the bacterial liquid bottle 26, a cover plate 23 is arranged horizontally on the top of the rotating disk 16, and six groups of sliding rods 18 are movably inserted under the rotating disk 16, two groups of ventilation fans 20 are installed at the rear of the constant temperature chamber 3, and two groups of shielding plates 22 are movably connected to the surface of the left side of the cabinet 1;

[0026] The rotating disk 16 can be rotated horizontally into the constant temperature chamber 3, the shielding plate 22 can close the left side of the constant temperature chamber 3, the top of the slide bar 18 is attached to the bottom of the bacterial liquid bottle 26, and the top of the slide bar 18 can slide up and down in the slot 25;

[0027] Specifically, Figure 1 and Figure 2 As shown, the rotary disk 16 connected to the rotating shaft 21 is turned on the left side of the constant temperature chamber 3 to pour the bacterial solution to be cultured into the bacterial solution bottle 26, and the bottle cap 24 is closed and placed in the slot 25 for storage, and then rotated into the constant temperature chamber 3, and the ventilation fan 20 is started to culture at a constant temperature. When taking it out, the rotary disk 16 is also turned out, and then the sliding rod 18 is lifted from the bottom to the top to push out the bacterial solution bottle 26, and the left side of the constant temperature chamber 3 is blocked by the rotating shielding plate 22.

[0028] Embodiment 2: A circular groove 5 is provided at the upper right corner of the exterior of the cabinet 1, and bulbs 6 are installed on both sides of the bottom of the circular groove 5, and movable chambers 4 are provided on both sides of the circular groove 5, and a plug rod 28 is transversely inserted between the movable chamber 4 and the circular groove 5, a clamping block 30 is fixedly connected to one side of the plug rod 28, a spring 29 is transversely fixed between the movable chamber 4 and the plug rod 28, and a grid line plate 27 is inserted in the circular groove 5;

[0029] The clamping blocks 30 are symmetrically clamped on both sides of the circular groove 5, and the insertion rod 28 is embedded in the spring 29. The insertion rod 28 can be inserted between the active chamber 4 and the circular groove 5, and the insertion rod 28 can slide horizontally along the inner wall of the active chamber 4;

[0030] Specifically, Figure 1 , Figure 3 and Figure 4 As shown, the rod 28 is moved horizontally along the movable chamber 4 on both sides to guide the symmetrical block 30 to slide along the side wall of the circular groove 5, and the coated and grown plates are placed into the circular groove 5 one by one, and then the rod 28 is released. Under the retracting force of the spring 29, the placed plates can be clamped, and the grid line plate 27 is placed above the glass plate, and then the bulbs 6 on both sides below are turned on to provide light, so as to facilitate the counting of bacterial species.

[0031] Embodiment 3: A storage groove 19 is horizontally arranged inside the lower left corner of the cabinet 1, and an operating table 17 is movably inserted in the storage groove 19, a frame 7 is fixed on the left side of the outside of the cabinet 1, and a liquid tank 8 is installed on the top of the outside of the frame 7, a liquid pump 10 is installed on the upper left side of the outside of the liquid tank 8, a shunt pipe 13 is fixed on the outer wall of the left side of the frame 7, a spray pipe 14 is fixed on the inner wall of the left side of the frame 7, and a spray plate 15 is fixed on the right side of the outside of the spray pipe 14, a liquid pipe 11 is connected between the output end of the liquid pump 10 and the shunt pipe 13, a purple light lamp 9 is horizontally fixed on the top of the frame 7, a glass plate 12 is installed at the rear of the frame 7, the shunt pipe 13 is connected to the spray pipe 14, and the liquid pipe 11 is connected between the liquid pump 10 and the shunt pipe 13;

[0032] Specifically, Figure 1 As shown, the glass plate 12 at the rear of the frame 7 is opened, and the operating table 17 is pulled out from the storage groove 19 at the bottom to perform operations such as inoculation, coating, and pipetting. After starting and ending the operation, the glass plate 12 can be closed, and the liquid pump 10 can be started. The disinfectant liquid is pumped from the liquid tank 8 to the shunt pipe 13 through the liquid pipe 11, and then sprayed on the inner wall of the frame 7 through multiple spray discs 15, and then the ultraviolet lamp 9 is started for dozens of minutes of irradiation to jointly eliminate the germs inside the frame 7.

[0033] Working principle: When the utility model is used, first open the glass plate 12 behind the frame 7, and pull out the operating table 17 from the storage slot 19 at the bottom to perform operations such as inoculation, coating, and pipetting. After starting and ending the operation, the glass plate 12 can be closed, and the liquid pump 10 can be started to draw disinfectant from the liquid tank 8 to the shunt pipe 13 through the liquid pipe 11, and spray it on the inner wall of the frame 7 through multiple spray discs 15, and then start the ultraviolet light lamp 9 for dozens of minutes of irradiation to jointly eliminate the pathogens inside the frame 7, and then turn the rotary disk 16 connected to the rotating shaft 21 on the left side of the constant temperature chamber 3 to pour the bacterial liquid to be cultured into the bacterial liquid bottle 26, close the bottle cap 24 and put it into the slot 25 together. Storage, then rotate it into the constant temperature chamber 3, start the ventilation fan 20 to cultivate at a constant temperature, and when taking it out, turn the rotary disk 16, then lift the slide bar 18 from bottom to top to push out the bacterial liquid bottle 26, and the left side of the constant temperature chamber 3 is blocked by the rotating baffle plate 22, and finally move the insertion rod 28 horizontally along the movable chamber 4 on both sides, guide the symmetrical card block 30 to slide along the side wall of the circular groove 5, put the coated and grown plates into the circular groove 5 one by one, and then release the insertion rod 28. Under the retraction force of the spring 29, the placed plates can be clamped, and the grid line plate 27 is placed above the glass plate, and then the bulbs 6 on both sides below are turned on to provide light, which is convenient for counting the strains.

[0034] It is obvious to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, the embodiments should be regarded as exemplary and non-limiting from any point of view, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims are included in the present invention. Any reference numeral in a claim should not be regarded as limiting the claim to which it relates.

Claims

1. A high-throughput RCL screening device, comprising a cabinet (1) and a spectrometer (2), characterized in that: A spectrometer (2) is installed at the lower right corner of the cabinet (1), two groups of constant temperature chambers (3) are transversely arranged on the left side of the cabinet (1), and a rotating shaft (21) is movably connected between the upper and lower parts of the left front of the constant temperature chamber (3), a rotating disk (16) is transversely fixed on the left side of the rotating shaft (21), and six groups of slots (25) are arranged inside the rotating disk (16), a bacterial liquid bottle (26) is inserted in the slot (25), and a bottle cap (24) is inserted on the top of the bacterial liquid bottle (26), a cover plate (23) is transversely inserted on the top of the rotating disk (16), and six groups of sliding rods (18) are movably inserted below the rotating disk (16), two groups of ventilation fans (20) are installed at the rear of the constant temperature chamber (3), and two groups of shielding plates (22) are movably connected to the surface of the left side of the cabinet (1).

2. The high-throughput RCL screening device according to claim 1, characterized in that: The rotating disk (16) can be rotated horizontally into the constant temperature chamber (3), and the shielding plate (22) can close the left side of the constant temperature chamber (3).

3. The high-throughput RCL screening device according to claim 1, characterized in that: The top of the slide bar (18) is attached to the bottom of the bacterial liquid bottle (26), and the top of the slide bar (18) can slide up and down in the slot (25).

4. The high-throughput RCL screening device according to claim 1, characterized in that: A circular groove (5) is provided at the upper right corner of the exterior of the cabinet (1), and light bulbs (6) are installed on both sides of the bottom of the circular groove (5), and movable chambers (4) are provided on both sides of the circular groove (5), and a plug rod (28) is transversely inserted between the movable chamber (4) and the circular groove (5), a clamping block (30) is fixedly connected to one side of the plug rod (28), a spring (29) is transversely fixed between the movable chamber (4) and the plug rod (28), and a grid line plate (27) is inserted in the circular groove (5).

5. The high-throughput RCL screening device according to claim 4, characterized in that: The clamping blocks (30) are symmetrically clamped on two sides of the circular groove (5), and the insertion rod (28) is embedded in the spring (29).

6. The high-throughput RCL screening device according to claim 4, characterized in that: The insertion rod (28) can be inserted between the active chamber (4) and the circular groove (5), and the insertion rod (28) can slide horizontally along the inner wall of the active chamber (4).

7. The high-throughput RCL screening device according to claim 1, characterized in that: A storage groove (19) is transversely arranged inside the lower left corner of the cabinet (1), and an operating table (17) is movably inserted in the storage groove (19); a frame (7) is fixed on the left side of the outside of the cabinet (1), and a liquid tank (8) is installed on the top of the outside of the frame (7); a liquid pump (10) is installed on the upper left side of the outside of the liquid tank (8); a shunt pipe (13) is fixed on the outer wall of the left side of the frame (7); a spray pipe (14) is fixed on the inner wall of the left side of the frame (7), and a spray disc (15) is fixed on the right side of the outside of the spray pipe (14); a liquid pipe (11) is connected between the output end of the liquid pump (10) and the shunt pipe (13); a purple light lamp (9) is transversely fixed on the top of the frame (7); and a glass plate (12) is installed at the rear of the frame (7).

8. The high-throughput RCL screening device according to claim 7, characterized in that: The shunt pipe (13) is connected to the spray pipe (14), and the liquid pipe (11) is connected between the liquid pump (10) and the shunt pipe (13).

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