Purification device for measuring content of sudan red in chilli sauce based on LC-MS / MS method

Abstract

The utility model discloses a purification device for determining the content of Sudan red in chilli sauce based on an LC-MS / MS (liquid chromatography-mass spectrometry / mass spectrometry) method, and belongs to the field of Sudan red extraction equipment. Comprising a vortex extractor body, an upper pressing plate is arranged on the vortex extractor body, a pressing block is arranged on the lower end face of the upper pressing plate, and a plurality of fastening grooves are formed in the lower end face of the pressing block in an array mode. The device has the advantages that the fastening groove is formed in the lower end of the pressing block, the upper end face of the test tube is arranged in the fastening groove, and the test tube can be prevented from slipping. The test tube is made of glass and is easy to break under a pressed condition, so that the flexible layer is padded on the upper groove surface of the fastening groove, and the test tube can be prevented from being damaged due to excessive pressure. Due to the fact that a test tube needs to extend into the fastening groove of the device, the test tube cannot be completely attached to the side wall of the fastening groove, the test tube can continuously collide with the wall of the fastening groove during high-speed rotating oscillation, the collision can be greatly relieved through the arranged flexible ring, and the test tube is prevented from being damaged.

Description

Technical Field

[0001] The utility model relates to a purification device for determining the Sudan red content in chili sauce based on an LC-MS / MS method, and belongs to the field of Sudan red extraction equipment. Background Art

[0002] Sudan red is an azo-based synthetic dye that is carcinogenic. Both the European Union and my country have banned the use of Sudan red dyes in food. However, some vendors still add it to food, especially peppers; so there is a method to quickly detect Sudan red.

[0003] The method refers to the Chinese invention patent application number CN201811654176.3 "A method for detecting Sudan red in food". This method adopts the LC-MS / MS method.

[0004] The first step of the method is to pre-treat the food sample to remove impurities in the sample to obtain a test solution. This step is also called Sudan red extraction. The method used for Sudan red extraction in the patent is:

[0005] The food sample and the organic extraction reagent are placed in an extraction bottle, and the food sample and the organic extraction reagent are centrifuged to obtain a solution including a supernatant and allow Sudan red in the food sample to be extracted into the supernatant;

[0006] At the same time, before centrifuging the food sample and the organic extraction reagent, the food sample and the organic extraction reagent can be mixed by manually shaking the extraction bottle or vortexing the extraction bottle for a period of time (e.g., 30 seconds) to promote the centrifugation effect.

[0007] For the vortex instrument, you can refer to "CN202222064597.9 A multi-tube vortex instrument". The multi-tube vortex instrument uses an upper baffle to constrain the test tube. The upper baffle is pressed on the upper end of the test tube to facilitate the test tube to remain stable during rotation and oscillation.

[0008] The applicant believes that there can be a better implementation structure for the stability of the test tube. Utility Model Content

[0009] The utility model aims to provide a purification device for determining the Sudan Red content in chili sauce based on the LC-MS / MS method, which can effectively solve the above-mentioned problems.

[0010] In order to solve the above technical problems, the utility model is achieved through the following technical solutions:

[0011] It comprises a vortex extractor body; an upper pressing plate is arranged on the vortex extractor body, a pressing block is arranged on the lower end surface of the upper pressing plate, and a plurality of fastening grooves are arranged in an array on the lower end surface of the pressing block.

[0012] Further: the upper groove surface of the fastening groove is provided with a flexible layer.

[0013] Furthermore: a flexible ring is also provided on the inner wall of the fastening groove.

[0014] Further: the lower end surface of the flexible ring is provided with a rounded corner.

[0015] Furthermore: the flexible layer and the flexible ring are made of one of rubber, plastic and silicone.

[0016] Furthermore: the flexible ring and the flexible layer are integrally formed.

[0017] Further: the vortex extractor body also includes a box body, springs are arranged at the four corners of the upper end surface of the box body, a bearing plate is arranged on the upper end surface of the spring, a test tube storage plate is arranged on the upper end surface of the bearing plate, and test tube slots are arrayed in the test tube storage plate, and the test tube slots correspond to the upper and lower positions of the fastening slots; two sliding rods are also arranged on the upper end surface of the box body, and the upper pressure plate sliding sleeve is arranged on the sliding rods; an electric motor is arranged in the box body, a transmission shaft is connected to the motor, a cam is arranged on the transmission shaft, and the cam is connected to the bearing plate through a connecting shaft.

[0018] Furthermore: the upper pressure plate and the slide rod are connected via a sliding sleeve, and a clamping nut is provided on the sliding sleeve.

[0019] Further: the sliding rods have different lengths.

[0020] The beneficial effects are:

[0021] This device makes improvements to the upper pressing plate. The reason for the improvement is that the lower end surface of the original upper pressing plate is smooth, and only relies on pressure to act on the upper end surface of the test tube. At this time, the test tube may slip during the high-speed rotation and vibration process;

[0022] The device is provided with a fastening groove at the lower end of the pressing block, and the upper end surface of the test tube is placed in the fastening groove, which can prevent the test tube from slipping.

[0023] Since the test tubes are made of glass, they are easy to break under pressure, so a flexible layer is placed on the upper groove surface of the fastening groove to prevent the test tubes from being damaged due to excessive pressure.

[0024] Since the fastening groove of the device requires the test tube to be inserted, the test tube will not completely fit the side wall of the fastening groove. During high-speed rotation and oscillation, the test tube will continuously collide with the fastening groove wall. The flexible ring can greatly reduce this collision and prevent the test tube from being damaged. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] For ease of explanation, the present invention is described in detail by the following specific implementations and drawings.

[0026] Figure 1 It is a schematic diagram of the structure of the utility model;

[0027] Figure 2 It is the parts diagram of the upper pressing plate of the utility model;

[0028] Figure 3 It is a cross-sectional view of the fastening groove of the utility model.

[0029] Description of reference numerals:

[0030] 1. Vortex extractor body; 2. Upper pressure plate; 3. Pressure block; 4. Fastening groove; 5. Flexible layer; 6. Flexible ring; 7. Fillet; 8. Box body; 9. Spring; 10. Loading plate; 11. Test tube storage plate; 12. Sliding rod; 13. Motor; 14. Transmission shaft; 15. Cam; 16. Connecting shaft; 17. Sliding sleeve; 18. Pressing nut. DETAILED DESCRIPTION

[0031] The embodiments of the present invention are described in detail below, and examples of the embodiments are shown in the accompanying drawings. The embodiments described below with reference to the accompanying drawings are exemplary and intended to be used to explain the present invention, but cannot be understood as limiting the present invention.

[0032] See also Figure 1-3 This is an embodiment of the utility model of a purification device for determining the Sudan red content in chili sauce based on the LC-MS / MS method.

[0033] It includes a vortex extractor body 1; the vortex extractor body 1 includes a box body 8, springs 9 are arranged at the four corners of the upper end surface of the box body 8, a bearing plate 10 is arranged on the upper end surface of the spring 9, a test tube storage plate 11 is arranged on the upper end surface of the bearing plate 10, and test tube slots are arrayed in the test tube storage plate 11, and the upper and lower positions of the test tube slots and the fastening slots 4 correspond; two slide bars 12 are also arranged on the upper end surface of the box body 8, and the sliding sleeve of the upper pressure plate 2 is arranged on the slide bar 12; an electric motor 13 is arranged in the box body 8, a transmission shaft 14 is connected to the electric motor 13, a cam 15 is arranged on the transmission shaft 14, and the cam 15 is connected to the bearing plate 10 through a connecting shaft 16.

[0034] An upper pressing plate 2 is also provided on the vortex extractor body 1 , a pressing block 3 is provided on the lower end surface of the upper pressing plate 2 , and a plurality of fastening grooves 4 are arranged in an array on the lower end surface of the pressing block 3 .

[0035] This device mainly relies on the LC-MS-MS method to determine the Sudan red content in the chili sauce. Before the determination, the Sudan red needs to be extracted. The Sudan red in the food sample is extracted into the supernatant using an organic extraction reagent. The steps include:

[0036] Place a food sample and an organic extraction reagent in an extraction bottle; centrifuge the food sample and the organic extraction reagent to obtain a solution including a supernatant and extract the Sudan red in the food sample into the supernatant. The order in which the food sample and the organic extraction reagent are placed in the extraction bottle will not affect the test results. It can be the food sample first and then the organic extraction reagent, or it can be the organic extraction reagent first and then the food sample. The food sample and the organic extraction reagent can also be placed in the extraction bottle at the same time. Before centrifuging the food sample and the organic extraction reagent, the food sample and the organic extraction reagent can be mixed by manually shaking the extraction bottle or extracting with the vortex extractor body 1 for a period of time (for example, 30 seconds), and then centrifuged. After the centrifugation is completed, the supernatant obtained is the organic solvent containing Sudan red.

[0037] The organic extraction reagent includes at least one of ethyl acetate, acetone, n-hexane, methanol, acetonitrile and anhydrous ethanol.

[0038] After the extraction was completed, the supernatant was detected using LC-MS-MS.

[0039] The specific operation process of the vortex extractor body 1 is as follows: the organic solvent and the chili sauce are mixed and put into a test tube, and then the test tube is placed in the test tube slot of the test tube storage plate 11, and then the upper end of the test tube is pressed by the upper pressing plate 2, and the motor 13 is turned on. The motor 13 drives the cam 15 to rotate through the transmission shaft 14, and the cam 15 drives the connecting shaft 16 to rotate. At this time, the entire carrier plate 10 will rotate, thereby driving all the test tubes to move, and the liquid in the test tube will form a vortex, which can dissolve the Sudan red in the chili sauce; after the rotation is completed, let it stand for a period of time, and a supernatant containing Sudan red can be obtained.

[0040] The device makes an improvement on the upper pressing plate 2. The reason for the improvement is that the lower end surface of the original upper pressing plate 2 is smooth, and only relies on pressure to act on the upper end surface of the test tube. At this time, the test tube may slip during the high-speed rotation and vibration process;

[0041] The device is provided with a fastening groove 4 at the lower end of the pressing block 3, and the upper end surface of the test tube is placed in the fastening groove 4, which can prevent the test tube from slipping.

[0042] The upper groove surface of the fastening groove 4 is provided with a flexible layer 5 .

[0043] Since the test tubes are made of glass, they are easy to break under pressure, so a flexible layer 5 is padded on the upper groove surface of the fastening groove 4 to prevent the test tubes from being damaged due to excessive pressure.

[0044] A flexible ring 6 is also provided on the inner wall of the fastening groove 4 .

[0045] Since the fastening groove 4 of the device requires the test tube to be inserted, the test tube will not completely fit the side wall of the fastening groove 4. During high-speed rotation and oscillation, the test tube will continuously collide with the wall of the fastening groove 4. The flexible ring 6 can greatly reduce this collision and prevent the test tube from being damaged.

[0046] The lower end surface of the flexible ring 6 is provided with a rounded corner 7 .

[0047] The diameter of the upper end of the test tube is slightly larger than the inner diameter of the flexible ring 6 , so the rounded corner 7 can facilitate the insertion of the test tube into the flexible ring 6 .

[0048] The flexible layer 5 and the flexible ring 6 are made of rubber, plastic or silicone.

[0049] The flexible layer 5 and the flexible ring 6 of the device are made of rubber, which has low cost and stable properties.

[0050] The flexible ring 6 is formed integrally with the flexible layer 5 .

[0051] Such an arrangement can facilitate the processing of the flexible ring 6 and the flexible layer 5, so as to reduce the cost.

[0052] The upper pressing plate 2 and the sliding rod 12 are connected via a sliding sleeve 17 , and a clamping nut 18 is provided on the sliding sleeve 17 .

[0053] The clamping nut 18 cooperates with the sliding sleeve 17 to limit the horizontal position of the upper pressing plate 2 .

[0054] The slide bars 12 are of different lengths.

[0055] The slide bar 12 on the left side of the device is longer. When the upper pressing plate 2 moves upward, one side will not be separated from the slide bar 12, while the other side will be separated from the shorter slide bar 12. After separation, the upper pressing plate 2 can rotate around the longer slide bar 12, making it convenient to place the test tube in the test tube storage plate 11.

[0056] The above description is only a specific embodiment of the present invention, but the technical features of the present invention are not limited thereto. Any changes or modifications made by any technician in the field of the present invention are included in the patent scope of the present invention.

Claims

1. A purification device for determining the Sudan red content in chili sauce based on LC-MS / MS method, characterized in that: The invention comprises a vortex extractor body (1); an upper pressure plate (2) is arranged on the vortex extractor body (1); a pressure block (3) is arranged on the lower end surface of the upper pressure plate (2); a plurality of fastening grooves (4) are arranged in an array on the lower end surface of the pressure block (3); a flexible layer (5) is arranged on the upper groove surface of the fastening groove (4); and a flexible ring (6) is also arranged on the inner wall of the fastening groove (4).

2. The purification device for determining the Sudan Red content of chili sauce based on the LC-MS / MS method according to claim 1, characterized in that: The lower end surface of the flexible ring (6) is provided with a rounded corner (7).

3. The purification device for determining the Sudan Red content of hot pepper sauce based on the LC-MS / MS method according to claim 2, characterized in that: The flexible layer (5) and the flexible ring (6) are made of one of rubber, plastic and silicone.

4. The purification device for determining the Sudan Red content of hot pepper sauce based on the LC-MS / MS method according to claim 3, characterized in that: The flexible ring (6) and the flexible layer (5) are integrally formed.

5. The purification device for determining the Sudan Red content of hot pepper sauce based on LC-MS / MS method according to claim 4, characterized in that: The vortex extractor body (1) also includes a box body (8), springs (9) are arranged at the four corners of the upper end surface of the box body (8), a bearing plate (10) is arranged on the upper end surface of the spring (9), and a test tube storage plate (11) is arranged on the upper end surface of the bearing plate (10), and the test tube storage plate (11) is arrayed with test tube slots, and the test tube slots correspond to the upper and lower positions of the fastening slots (4); two sliding rods (12) are also arranged on the upper end surface of the box body (8), and the sliding sleeves of the upper pressure plate (2) are arranged on the sliding rods (12); an electric motor (13) is arranged in the box body (8), and a transmission shaft (14) is connected to the electric motor (13), and a cam (15) is arranged on the transmission shaft (14), and the cam (15) and the bearing plate (10) are connected via a connecting shaft (16).

6. The purification device for determining the Sudan Red content in chili sauce based on the LC-MS / MS method according to claim 5, characterized in that: The upper pressing plate (2) and the sliding rod (12) are connected via a sliding sleeve (17), and a clamping nut (18) is provided on the sliding sleeve (17).

7. The purification device for determining the Sudan Red content in chili sauce based on the LC-MS / MS method according to claim 6, characterized in that: The sliding rods (12) have different lengths.

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