Tool for potato micro-branch grafting

Abstract

The utility model discloses a tool for potato micro-branch grafting. The tool comprises a pair of micro-grafting scissors, a tweezers sleeve, a silica gel sleeve and a sleeve assist device. The pair of micro-grafting scissors comprises a handle, scissor arms, a screw, a cutter clamp, operating cutters and an operating cutter supporting frame, the two arms of the micro-grafting scissors are arranged in a crossed mode, the screw is connected to the position close to the front ends of the two scissor arms, the cutter clamp is located at the front ends of the scissor arms, the operating cutter supporting frame is connected to the cutter clamp, and the cutter clamp is used for disassembling and replacing the operating cutters of different specifications. The cannula assist device comprises kneading parts, an elastic piece and an operating needle, the kneading parts are connected at the top ends of one ends, the elastic piece is made of elastic materials and connected between the two kneading parts, so that the cannula assist device is in an open state under the natural condition, and the operating needle is connected to the open ends of the kneading parts. The utility model provides a set of tool for reducing the operation difficulty of potato micro-branch grafting, effectively improving the grafting efficiency and the survival rate, and reducing the operation time and the waste of grafting materials.

Description

Technical Field

[0001] The utility model belongs to the technical field of potato microbranch grafting, in particular to a set of tools used for potato microbranch grafting. Background Art

[0002] Micrografting refers to the technology of grafting the stem segment of a test tube seedling onto another topped test tube seedling or stem segment under sterile conditions and cultivating it into a new plant under suitable conditions. It is an asexual reproduction technology that combines grafting technology with plant tissue culture. Micrografting technology is not restricted by seasons and environments and can be carried out in the laboratory all year round. It is mainly used for graft affinity identification, rapid detection and detoxification of plant viruses, rapid breeding of virus-free seedlings, and breeding and preservation of precious breeding materials and germplasm resources.

[0003] Microbranch grafting technology is currently mainly used on fruit trees, and its application in potatoes is relatively rare. Grafting technology can also be used in potato breeding to improve its resistance, yield and quality. The specific practice of the existing potato microbranch grafting technology is: select sturdy potato test tube seedlings, use a scalpel to cut off 1 cm of the top bud of the rootstock material, and cut the incision without the top bud into a "V" shape, "I" shape or " / " shape; at the same time, use a scalpel to cut off 1-2 cm of the top bud of the scion material, and cut the incision with the top bud into a shape that can completely fit the rootstock material. Use tweezers to fit the incisions of the rootstock and scion materials together, and then use tweezers to clamp the tin foil and wrap it around the interface to fix the rootstock and scion to form a chimeric material; finally, insert the chimeric material into the MS culture medium, and after the grafting is healed, remove the tin foil to obtain a potato microbranch grafted plant. During the entire operation, it is very difficult to cut a specific shape at the grafting site of the potato test tube seedling material with a scalpel. The stock and the scion may not fit completely due to the cut shape, which reduces the contact surface and leads to a low survival rate of the chimera material. Since the cutting process takes a long time, the material may also lose water, resulting in grafting failure. This scalpel cutting process is generally not possible in the original culture bottle, so the rootstock with roots in the original culture bottle cannot be grafted. In addition, when the scion is clamped with tweezers, the scion site is usually the tender apical bud site, and the iron tweezers can easily damage it. Finally, in the process of fixing the grafting site with tin foil, the stock and scion that are attached together are often separated, and the healing of the stock and scion cannot be observed in real time after the tin foil is wrapped. In order to solve these problems, this utility model aims to be able to cut the stock and scion materials faster, more conveniently and more accurately, reduce the damage to the tender grafting materials, and observe the healing of the grafting interface in real time, thereby greatly improving the efficiency of potato microbranch grafting operation and the survival rate of grafted plants. Utility Model Content

[0004] The utility model aims to solve the problem that in the process of potato micro-branch grafting, the operation process is difficult, the survival rate of grafted plants is low, and a lot of time and materials are wasted. The utility model provides a set of tools to reduce the difficulty of micro-branch grafting operation, improve the grafting efficiency and survival rate, and reduce the waste of time and materials.

[0005] The purpose of the utility model is achieved by adopting the following technical scheme: a set of tools for potato micro-branch grafting, including micro-grafting scissors, tweezers, silicone sleeves, and sleeve assistants. The micro-grafting scissors include a handle, a scissor arm, a screw, a knife clamp, an operating knife, and an operating knife support frame. The micro-grafting scissors are arranged with two arms crossed, the handles are respectively located at the rear ends of the two arms, the screws are connected at a position close to the front ends of the two scissor arms, the knife clamp is located at the front ends of the scissor arms, and the operating knife support frame is connected to the knife clamp, and the knife clamp is used to disassemble and replace operating knives of different specifications. The tweezers cover is made of silicone, and is a hollow structure with a large upper part and a small lower part, which can be completely fitted on the tips of the two arms of the tweezers. The silicone sleeve is made of transparent silicone material, and is a cylindrical hollow structure, and the longitudinal side of the silicone sleeve has a longitudinal opening. The sleeve auxiliary device includes a kneading part, a spring sheet, and an operating needle. The kneading part is connected at the top end at one end. The spring sheet is made of elastic material and connected between the two kneading parts, so that the sleeve auxiliary device is in an open state under natural conditions. The operating needle is connected to the open end of the kneading part.

[0006] Preferably, the handle is ring-shaped and provided with an inner ring, and the inner ring is a silicone pad.

[0007] Preferably, the distance between the rear end of the scissor arm and the screw is twice the distance between the front end of the scissor arm and the screw.

[0008] Preferably, the knife card is located 1 cm from the front end of the scissor arm, and there are protruding iron sheets on both sides 1 cm from the front end of the scissor arm to form a groove, and there are two screw holes at the upper and lower ends of the knife card; there are two screw holes on the operating knife support frame corresponding to the screw holes on the knife card, and the operating knife support frame can completely fit into the groove of the knife card; the operating knife support frame is connected to the knife card by two screws.

[0009] Preferably, the operating knife includes a placement slot and a cutting knife, and the placement slot and the cutting knife are respectively fixed to the front end of the scissor arm through knife clamps; there are 2mm protruding iron sheets on both sides of the placement slot to form a concave; a cutting notch is provided in the middle of the concave of the placement slot; the specifications of the cutting knife correspond to the cutting notch, the cutting knife seat is 0.5mm wider than the inner diameter of the placement slot, and the blade height is fixed at 2.5mm; the cutting knife seat will be stuck at the top of the placement slot, and the blade can pass through the cutting notch in the placement slot and protrude 0.5mm; the blade of the cutting knife corresponds to the shape of the cutting notch in the placement slot.

[0010] Preferably, the width of the inner concave of the placement groove is divided into four specifications: 1mm, 1.3mm, 1.5mm, and 2mm.

[0011] Preferably, the cutting notch in the middle of the inner concave portion of the placement groove is in a "V" shape, a " / " shape, or a "-" shape.

[0012] Preferably, it also includes a tweezers cover, which can be respectively covered on the tips of the two arms of the tweezers; the tweezers cover is a hollow frustoconical hose made of silicone, and the tweezers cover is 1 cm long and 2 mm thick.

[0013] Preferably, it also includes a silicone sleeve, which is a transparent hose made of silicone, with a length of 1 cm, a thickness of 0.5 mm, high transparency and clarity, and an inner diameter of the silicone sleeve of four specifications: 1 mm, 1.3 mm, 1.5 mm, and 2 mm; the silicone sleeve has a longitudinal opening on the longitudinal side, and the longitudinal opening can be stretched open so that the silicone sleeve is sleeved on the interface of the potato test tube seedling grafting material that is slightly larger than the inner diameter of the silicone sleeve.

[0014] Preferably, it also includes a cannula assistant, which includes a kneading part, a spring sheet, and an operating needle, and the kneading part is connected at one end; the spring sheet is made of elastic material and connected between the two kneading parts, so that the cannula assistant is in an open state under natural conditions; the operating needle is connected to the open end of the kneading part.

[0015] Beneficial effects:

[0016] 1. The micro-grafting shears can quickly and accurately cut the potato test tube seedling rootstock and scion material into a "V" shape, " / " shape or "I" shape, so that the rootstock and scion material can fit together completely, greatly improving the grafting efficiency and survival rate.

[0017] 2. The micro-grafting shears can be replaced with operating knives of different specifications according to needs, which saves costs and can be used for cutting rootstocks and scions of potato test tube seedlings or other plant materials of different thicknesses.

[0018] 3. The soft texture of the tweezers cover reduces the damage of iron tweezers to potato test tube seedling rootstocks and young scion materials.

[0019] 4. The transparent silicone sleeve can better fix the interface of the grafting material, and the healing condition of the grafted seedling interface can be observed in real time.

[0020] 5. The sleeve auxiliary device can quickly open the longitudinal side of the silicone sleeve, making it convenient for the silicone sleeve to be accurately placed on the interface of the grafting material, thus playing a good role in fixing it. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is an exploded view of the micro-grafting shears;

[0022] Figure 2 yes Figure 1A-direction view;

[0023] Figure 3 yes Figure 1 B-direction view;

[0024] Figure 4 It is a schematic diagram of the structure of micro-grafting shears;

[0025] Figure 5 is a schematic diagram of the structure of an operating knife with a "V"-shaped indentation;

[0026] Figure 6 1. It is a schematic diagram of the structure of an operating knife with a notch in the shape of " / ";

[0027] Figure 7 It is a schematic diagram of the structure of an operating knife with a notch in the shape of "I";

[0028] Figure 8 It is a structural schematic diagram of a tweezers set;

[0029] Fig. 9 It is a schematic diagram of the structure of the silicone sleeve;

[0030] Fig.10 It is a schematic diagram of the structure of the casing auxiliary device. DETAILED DESCRIPTION

[0031] like Figure 1-10 As shown, the utility model discloses a set of tools for potato micro-branch grafting, including micro-grafting shears, tweezers sets, silicone sleeves and sleeve assistants.

[0032] 1. Micro-grafting shears:

[0033] 1. Handle 1: The inner ring contains a silicone pad, which acts as a buffer. When operating for a long time, it can prevent the handle 1 from squeezing the fingers and causing discomfort;

[0034] 2. Screw 3: used to fix the two scissor arms 2 of the micro-grafting scissors. Compared with ordinary scissors, the position of screw 3 is closer to the front end of the micro-grafting scissors, which is more labor-saving to operate;

[0035] 3. Knife card 4: used to disassemble and replace operating knives 6 of different specifications, located 1 cm from the front end of the scissor arm 2. There are 2 mm protruding iron sheets on both sides of the front end of the scissor arm 1 cm to form a groove, and there are two screw holes 5 at the upper and lower ends of the groove. The operating knife support frame 7 can completely fit the groove of the knife card 4, and the operating knife can be completely fixed by installing two screws.

[0036] 4. Operating knife 6: The operating knife 6 includes a placement slot 9 and a cutting knife 10, which are connected to the operating knife support frame 7 respectively, and are arranged on both sides, one side is the placement slot 9, and the other side is the cutting knife 10. There are two 2mm protruding iron sheets on both sides of the placement slot 9, forming an inner concave, and the inner concave of the placement slot 9 is opposite to the blade of the cutting knife 10. The inner width of the placement slot 9 is divided into four specifications of 1mm, 1.3mm, 1.5mm, and 2mm, which can cover the diameter of most potato test tube seedlings. There are "V"-shaped, " / "-shaped, or "I"-shaped cutting notches 11 in the middle of the placement slots 9 of different specifications, respectively, to ensure that the blade 12 of the cutting knife 10 is pressed into the cutting notch 11, and the stem of the potato test tube seedling can be completely cut off. The specification of the cutting knife 10 corresponds to the placement groove 9, the cutting knife seat 13 is 0.5mm wider than the inner diameter of the placement groove 9, and the cutting knife seat 13 has a blade 12 corresponding to the shape of the cutting notch 11 in the concave of the placement groove 9, and the height of the blade 12 is fixed at 2.5mm. When the micro-grafting shears are closed, the cutting knife seat 13 will be stuck on the top of the placement groove 9, and the blade 12 will cut off the potato test tube seedlings in the placement groove 9, and the blade 12 can pass through the cutting notch 11 and penetrate 0.5mm, ensuring that the blade will not be damaged when cutting the potato test tube seedling material.

[0037] 2. Tweezers cover: Made of pure silicone, high temperature resistant, and can be sterilized by wet heat. The tweezers cover is a hollow structure with a large top and a small bottom, which can be completely fitted on the tips of the two arms of the tweezers. The tweezers cover is 1cm long and 2mm thick. It is soft and elastic. It is put on the tips of the two arms of the tweezers to prevent the tweezers from damaging the tender tissues of the potato test tube seedlings.

[0038] 3. Silicone sleeve: A transparent hose made of silicone, which is resistant to high temperatures and can be sterilized with wet heat. The silicone sleeve is a cylindrical hollow structure with a longitudinal opening on the longitudinal side of the silicone sleeve. It is 1 cm in length and 0.5 mm in thickness. It is transparent and has high clarity. It can completely cover the interface of the grafting material, and the healing of the grafting interface can be observed at any time. The inner diameter of the silicone sleeve has four specifications: 1mm, 1.3mm, 1.5mm, and 2mm. Choose a silicone sleeve with a specification similar to the thickness of the grafting material. There is a longitudinal opening 14 on the longitudinal side of the silicone sleeve, which can be stretched open so that the silicone sleeve can be sleeved on the interface of the potato test tube seedling grafting material that is slightly larger than its inner diameter. Compared with inserting the material from the upper and lower ends of the silicone sleeve, it is easier to operate and less likely to damage the material after stretching it from the longitudinal opening 14. The interface of the grafting material can be completely fitted through subtle adjustments. The transparent material of the silicone sleeve allows the healing of the grafting interface to be observed at any time. 4. Sleeve assistant:

[0039] 1. The pinching part 15: The place where the fingers pinch, and the auxiliary device can be completely closed together by applying force. The outer surface has striped patterns, which can play a role in anti-slip.

[0040] 2. Elastic piece 16: Keeps the sleeve auxiliary device in an open state under natural conditions.

[0041] 3. Operating needle 17: After passing through the longitudinal incision of the silica gel sleeve, expands the silica gel sleeve.

[0042] During use, burn the micro-grafting scissors, forceps, and the operating needle of the sleeve auxiliary device with an alcohol lamp and let them cool naturally. Then select potato test-tube seedlings with a suitable diameter from the culture bottle, operate with the micro-grafting scissors at the required position, embed the stem of the potato test-tube seedling into the concave of the placement groove of the micro-grafting scissors, and then pinch the handle of the micro-grafting scissors with two fingers to cut off the potato test-tube seedling. The cut end is in the shape of the selected cutting knife ("V" shape or " / " shape or "-" shape). If the rootstock is needed, discard the cut part. If the scion is needed, take out the cut part and place it in a petri dish lined with wet filter paper for later use. Put the forceps sleeves on the tips of the two arms of the forceps, use the forceps to pick up the scion material and make it completely fit with the cut of the rootstock material. Then select a suitable sleeve auxiliary device, pinch the sleeve auxiliary device to make the operating needle pass through the longitudinal incision of the silica gel sleeve, and slowly release the sleeve auxiliary device. The operating needle on the sleeve auxiliary device will expand the longitudinal side of the silica gel sleeve. Put the silica gel sleeve on the interface of the grafting material from the open position, and after fine adjustment, make the interface of the grafting material fit completely. Then slowly withdraw the sleeve auxiliary device, and the silica gel sleeve will fix the interface of the grafting material well together. Place the fixed grafting material under suitable conditions for cultivation, and observe the healing condition of the interface through the transparent silica gel sleeve in real time. After the interface is completely healed, use the operating needle of the sleeve auxiliary device to expand along the opening on the longitudinal side of the silica gel sleeve and take it out, then a potato micro-grafted seedling is obtained.

[0043] The above specific implementation manners do not constitute a limitation to the protection scope of the present utility model. Those skilled in the art should understand that various modifications, combinations, sub-combinations, and substitutions can occur depending on design requirements and other factors. Any modifications, equivalent substitutions, and improvements made within the spirit and principle of the present utility model shall be included within the protection scope of the present utility model.

Claims

1. A set of tools for potato micrografting, characterized in that: The micro-grafting scissors include a handle (1), a scissor arm (2), a screw (3), a knife clamp (4), an operating knife (6) and an operating knife support frame (7). The micro-grafting scissors are arranged with two arms crossed, the handle (1) is respectively located at the rear ends of the two arms, the screw (3) is connected to a position close to the front ends of the two scissor arms (2), the knife clamp (4) is located at the front ends of the scissor arms (2), the operating knife support frame (7) is located in the middle of the operating knife (6) and forms an angle of 45° with the operating knife (6), the operating knife support frame (7) is connected to the knife clamp (4), and the knife clamp (4) is used to disassemble and replace operating knives (6) of different specifications.

2. The tool for potato micrografting according to claim 1, characterized in that: The handle (1) is ring-shaped and is provided with an inner ring, which is a silicone pad.

3. The tool for potato micrografting according to claim 1, characterized in that: The distance between the rear end of the scissor arm (2) and the screw (3) is twice the distance between the front end of the scissor arm (2) and the screw (3).

4. The tool for potato micrografting according to claim 1, characterized in that: The knife clamps (4) are respectively located 1 cm from the front end of the scissor arms (2). A groove is formed by 2 mm protruding iron sheets on both sides of the front end of the scissor arms (2). Two screw holes (5) are provided at the upper and lower ends of the knife clamps (4). The operating knife support frame (7) is provided with two support frame screw holes (8) corresponding to the screw holes (5) on the knife clamps (4). The operating knife support frame (7) can completely fit into the groove of the knife feed clamp (4). The operating knife support frame (7) is connected to the knife clamp (4) by two screws.

5. The tool for potato micrografting according to claim 1, characterized in that: The operating knife (6) comprises a placement groove (9) and a cutting knife (10), and the placement groove (9) and the cutting knife (10) are respectively fixed to the front end of the scissor arm (2) through a knife clamp (4); 2mm protruding iron sheets are provided on both sides of the placement groove (9) to form an inner concave; a cutting notch (11) is provided in the middle of the inner concave of the placement groove (9); the specification of the cutting knife (10) corresponds to the cutting notch (11); the cutting knife seat (13) is 0.5mm wider than the inner diameter of the placement groove (9), and the height of the blade (12) is fixed at 2.5mm; the cutting knife seat (13) will be clamped on the top of the placement groove (9), and the blade (12) can pass through the cutting notch (11) in the placement groove (9) and protrude 0.5mm, and the blade (12) of the cutting knife (10) corresponds in shape to the cutting notch (11) in the placement groove (9).

6. The tool for potato micrografting according to claim 5, characterized in that: The inner concave width of the placement groove (9) is divided into four specifications: 1mm, 1.3mm, 1.5mm and 2mm.

7. The tool for potato micrografting according to claim 5, characterized in that: The cutting notch (11) in the middle of the inner concave portion of the placement groove (9) is in a "V" shape, a " / " shape, or a "-" shape.

8. The tool for potato micrografting according to claim 1, characterized in that: It also comprises a tweezer cover, which can be respectively covered on the tips of the two arms of the tweezers; the tweezer cover is a hollow truncated cone-shaped hose made of silica gel, with a length of 1 cm and a thickness of 2 mm.

9. The tool for potato micrografting according to claim 1, characterized in that: The invention also comprises a silicone sleeve, which is a cylindrical soft tube made of transparent silicone, with a length of 1 cm, a thickness of 0.5 mm, high transparency and clarity, and an inner diameter of the silicone sleeve of four specifications: 1 mm, 1.3 mm, 1.5 mm, and 2 mm; a longitudinal opening (14) is provided on the longitudinal side of the silicone sleeve, so that the silicone sleeve is sleeved on the interface of the potato test tube seedling grafting material which is slightly larger than the inner diameter of the silicone sleeve.

10. The tool for potato micrografting according to claim 1, characterized in that: The invention also comprises a cannula assistant, which comprises a kneading part (15), a spring sheet (16), and an operating needle (17). The kneading part (15) is connected at the top end; the spring sheet (16) is made of elastic material and connected between the two kneading parts (15), so that the cannula assistant is in an open state under natural conditions; the operating needle (17) is connected to the open end of the kneading part (15).

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