Microfluidic device for detecting drainage liquid amylase

Abstract

The utility model relates to the field of medical auxiliary instruments. The micro-fluidic device for detecting the amylase in the drainage liquid comprises a shell, the shell comprises a box body, the box body is provided with an inward concave area, and a micro-fluidic chip is installed in the inward concave area. A first lead-in pipe for leading in drainage liquid, a second lead-in pipe for leading in starch, a third lead-in pipe for leading in iodine liquid and a lead-out pipe for leading out waste liquid are fixed on the micro-fluidic chip; the micro-fluidic chip comprises a reaction area and a color development area which are adjacent to each other, the reaction area is provided with a reaction channel used for mixed reaction of drainage liquid and starch, the color development area is provided with a color development channel used for color development reaction with iodine liquid, the reaction channel is sequentially connected with the color development channel, and the reaction channel is respectively butted and communicated with the first ingress pipe and the second ingress pipe; one end, connected with the reaction channel, of the developing channel is butted and communicated with the third ingress pipe, and the other end of the developing channel is butted and communicated with the eduction pipe. According to the utility model, the uninterrupted detection in the drainage process of the drainage liquid is realized.

Description

Technical Field

[0001] The utility model relates to the field of medical auxiliary instruments, in particular to a detection device for amylase. Background Art

[0002] After abdominal surgery, patients usually need to leave a drainage tube to drain the residual or continued exudate in the abdominal cavity through the drainage to reduce abdominal infection. The properties and composition of the drainage fluid are important indicators for observing the pathological condition. When the drainage fluid contains pancreatic juice, it indicates pancreatic fistula. The presence of pancreatic juice is usually determined by measuring the amylase in the drainage fluid. Amylase is a hydrolase and is currently the most widely used enzyme in the fermentation industry.

[0003] There are many methods for determining amylase, and the iodine-starch colorimetric method is commonly used in clinical practice. Publication No. CN220099019U discloses a device for quickly detecting pancreatic amylase in drainage fluid. In this patent, the starch solution and iodine solution in the detection box are mixed and turn blue. The drainage tube is inserted into the drainage tube, and the drainage fluid in the drainage tube flows into the detection box through the drainage tube and mixes with the blue solution. If the drainage fluid contains pancreatic amylase, the amylase hydrolyzes the starch, and the mixed solution becomes lighter blue or becomes colorless; if the drainage fluid does not contain pancreatic amylase, the color of the mixed solution does not change.

[0004] The disadvantage is that it is impossible to achieve the drainage fluid to fully react with the starch and the iodine solution to change color in sequence during the flow process. Utility Model Content

[0005] In view of the problems existing in the prior art, the utility model provides a microfluidic device for detecting amylase in drainage fluid, which solves at least one of the above technical problems.

[0006] The technical solution of the utility model is: a microfluidic device for detecting drainage fluid amylase, comprising a shell, characterized in that the shell comprises a box body, a concave area is provided on the box body, a microfluidic chip is installed in the concave area, a first introduction tube for introducing drainage fluid, a second introduction tube for introducing starch, a third introduction tube for introducing iodine solution, and an outlet tube for exporting waste liquid are fixed on the microfluidic chip;

[0007] The microfluidic chip comprises a reaction zone and a color development zone arranged adjacent to each other, the reaction zone is provided with a reaction channel for a mixed reaction of drainage fluid and starch, the color development zone is provided with a color development channel for a color development reaction with iodine solution, the reaction channel is sequentially connected with the color development channel, and the reaction channel is connected to the first introduction tube and the second introduction tube respectively;

[0008] One end of the color development channel connected to the reaction channel is connected to the third introduction tube, and the other end of the color development channel is connected to the outlet tube;

[0009] The outlet pipe is connected to the inlet of the pump body through a hose, and the outlet of the pump body is connected to a waste liquid tank;

[0010] The shell body also includes a cover body for covering the opening of the concave area, and the cover body is hinged to the box body.

[0011] The utility model, through the arrangement of the microfluidic chip, facilitates the drainage fluid to sequentially pass through the reaction area mixed with starch and the color development area mixed with iodine solution, greatly improves the inspection effect of the drainage fluid, and realizes uninterrupted detection of the drainage fluid during the drainage process.

[0012] Further preferably, the box body comprises a base and a support frame, the base is provided with a slot for longitudinally inserting the support frame, and the support frame is plugged into the base;

[0013] The support frame and the base are fixed to each other by locking screws;

[0014] The microfluidic chip is installed on the supporting frame.

[0015] Further preferably, a boss is provided on the base, a support plate for overlapping the boss is provided on the support frame, a notch is provided on the support plate, and a positioning protrusion embedded in the notch is provided on the boss;

[0016] The locking screw is threadedly connected to the positioning protrusion, and the locking screw abuts against the supporting plate.

[0017] Further preferably, a placement groove for placing the battery is opened on the outer side of the base, and a cover for sealing the groove is slidably connected to the notch of the placement groove.

[0018] Further preferably, the support frame is provided with openings for exposing the reaction area and the color development area;

[0019] A heating plate for heating the reaction zone is installed on the base, and the heating plate is located directly below the reaction zone.

[0020] Further preferably, a photosensitive sensor and a light source are respectively installed on the upper and lower sides of the color display area, the photosensitive sensor is installed on the cover, and the light source is installed on the base.

[0021] The solution in the color development area will show different colors, which show different absorbance. The photosensitive sensor and the light source are directly opposite to the upper and lower sides of the color development area. The light of a specific wavelength generated by the light source passes through the color development area to reach the photosensitive sensor. The photosensitive sensor converts the received light signal into a digital signal and transmits it to the control chip. The control chip compares the digital signal with the calibrated threshold.

[0022] Further preferably, the first introduction tube is connected to a drainage tube extending out of the shell, and the shell is provided with a through hole for passing the drainage tube;

[0023] The second introduction tube is connected to a starch solution storage bag through a hose;

[0024] The third introduction tube is connected to an iodine solution storage bag through a hose.

[0025] Further preferably, the reaction channel is a circuitous serpentine pipeline, and the color development channel is a circuitous serpentine pipeline;

[0026] The first inlet pipe, the third inlet pipe and the outlet pipe are arranged in sequence along a first straight line direction;

[0027] The first introduction tube and the second introduction tube are arranged along a second straight line direction;

[0028] The first straight line direction is perpendicular to the second straight line direction.

[0029] Further preferably, the cover body is detachably connected to the box body via a locking mechanism.

[0030] Beneficial effects: The device combines microfluidic technology, which can reduce the volume of the detection device, while reducing the cost of detecting drainage fluid amylase and increasing the detection speed. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 This is a schematic diagram of a partial structure of the specific embodiment 1 of the utility model with the cover omitted;

[0032] Figure 2 It is a partial exploded view of the specific embodiment 1 of the utility model;

[0033] Figure 3 This is a partial structural diagram of the specific embodiment 1 of the utility model in which the cover is omitted;

[0034] Figure 4 It is a structural schematic diagram of specific embodiment 1 of the utility model.

[0035] In the figure: 1 is a base, 2 is a support frame, 3 is a cover body, 4 is a microfluidic chip, 5 is a pump body, 6 is a heating plate, 7 is a light source, 8 is a cover, 11 is a perforation, 21 is a first placement groove, 22 is a second placement groove, 41 is a first introduction tube, 42 is a second introduction tube, 43 is a third introduction tube, 44 is an outlet tube, 45 is a reaction channel, and 46 is a color development channel. DETAILED DESCRIPTION

[0036] See also Figures 1 to 4 Specific embodiment 1, a microfluidic device for detecting drainage fluid amylase, comprising a shell, the shell comprising a box body, a concave area is arranged on the box body, a microfluidic chip 4 is installed in the concave area, a first introduction tube 41 for introducing drainage fluid, a second introduction tube 42 for introducing starch, a third introduction tube 43 for introducing iodine solution and an outlet tube 44 for exporting waste liquid are fixed on the microfluidic chip 4; the microfluidic chip 4 comprises a reaction area and a color development area arranged adjacent to each other, the reaction area is provided with a reaction channel 45 for a mixed reaction of drainage fluid and starch, and the color development area The area is provided with a color development channel 46 for color development reaction with iodine solution, a reaction channel 45 is sequentially connected with the color development channel 46, and the reaction channel 45 is docked and connected with the first introduction tube 41 and the second introduction tube 42 respectively; one end of the color development channel 46 connected to the reaction channel 45 is docked and connected with the third introduction tube 43, and the other end of the color development channel 46 is docked and connected with the outlet tube 44; the outlet tube 44 is docked with the inlet of the pump body 5 through a hose, and the outlet of the pump body 5 is docked with a waste liquid tank; the housing also includes a cover body 3 for covering the opening of the concave area, and the cover body 3 is hinged with the box body. The utility model facilitates the drainage liquid to pass through the reaction area mixed with starch and the color development area mixed with iodine solution in sequence through the arrangement of the microfluidic chip 4, greatly improving the inspection effect of the drainage liquid and realizing uninterrupted detection during the drainage process of the drainage liquid.

[0037] The microfluidic chip is a plate supported by a transparent substrate. The first introduction tube, the second introduction tube, the third introduction tube and the outlet tube are all hollow steel needles.

[0038] The box body includes a base 1 and a support frame 2. The base 1 is provided with a slot for longitudinally inserting the support frame 2, and the support frame 2 is plugged into the base 1; the support frame 2 and the base 1 are fixed to each other by locking screws; a microfluidic chip 4 is installed on the support frame 2.

[0039] The base 1 is provided with a boss, the support frame 2 is provided with a support plate for overlapping the boss, the support plate is provided with a notch, and the boss is provided with a positioning protrusion embedded in the notch; the positioning protrusion is threadedly connected with a locking screw, and the locking screw is against the support plate. The outer side of the base 1 is provided with a placement groove for placing batteries, and the notch of the placement groove is slidably connected with a cover 8 for sealing the notch.

[0040] The support frame 2 is provided with openings for exposing the reaction zone and the color development zone; a heating plate 6 for heating the reaction zone is installed on the base 1, and the heating plate 6 is located directly below the reaction zone.

[0041] A photosensitive sensor and a light source 7 are installed on the upper and lower sides of the color development area, respectively. The photosensitive sensor is installed on the cover 3, and the light source 7 is installed on the base 1. It is convenient to realize that the solution in the color development area will present different colors, which show different absorbances. The photosensitive sensor and the light source 7 are directly opposite to the upper and lower sides of the color development area. The specific wavelength light generated by the light source 7 passes through the color development area to reach the photosensitive sensor. The photosensitive sensor converts the received light signal into a digital signal and transmits it to the control chip. The control chip compares the digital signal with the calibrated threshold.

[0042] The first introduction tube 41 is connected to the drainage tube extending out of the shell, and the shell is provided with a perforation 11 for passing the drainage tube; the second introduction tube is connected to a starch solution storage bag through a hose; the third introduction tube is connected to an iodine liquid storage bag through a hose. The support frame is divided into a first placement groove 21 and a second placement groove 22 arranged up and down by a partition. The first placement groove and the second placement groove are used to place the starch solution storage bag and the iodine liquid storage bag respectively. The back of the support frame provided with the first placement groove 21 and the second placement groove 22 is provided with a third placement groove for placing the waste liquid tank. The first placement groove, the second placement groove and the third placement groove are arranged adjacent to the support plate. The bag openings of the starch solution storage bag, the iodine liquid storage bag and the waste liquid bag are all equipped with a one-way valve.

[0043] The reaction channel 45 is a tortuous serpentine pipeline, and the color development channel 46 is a tortuous serpentine pipeline; the first inlet tube 41, the third inlet tube 43 and the outlet tube 44 are arranged in sequence along the first straight line direction; the first inlet tube 41 and the second inlet tube 42 are arranged along the second straight line direction; the first straight line direction is perpendicular to the second straight line direction.

[0044] The cover body 3 is detachably connected to the box body through a locking mechanism.

[0045] The above are only preferred implementations of the present invention. It should be pointed out that ordinary technicians in this technical field can make several improvements and modifications without departing from the principles of the present invention. These improvements and modifications should also be regarded as the protection scope of the present invention.

Claims

1. A microfluidic device for detecting amylase in drainage fluid, comprising a housing, characterized in that: The housing comprises a box body, a concave area is provided on the box body, a microfluidic chip is installed in the concave area, a first introduction tube for introducing drainage fluid, a second introduction tube for introducing starch, a third introduction tube for introducing iodine solution, and an outlet tube for outlet waste liquid are fixed on the microfluidic chip; The microfluidic chip comprises a reaction zone and a color development zone arranged adjacent to each other, the reaction zone is provided with a reaction channel for a mixed reaction of drainage fluid and starch, the color development zone is provided with a color development channel for a color development reaction with iodine solution, the reaction channel is sequentially connected with the color development channel, and the reaction channel is connected to the first introduction tube and the second introduction tube respectively; One end of the color development channel connected to the reaction channel is connected to the third introduction tube, and the other end of the color development channel is connected to the outlet tube; The outlet pipe is connected to the inlet of the pump body through a hose, and the outlet of the pump body is connected to a waste liquid tank; The shell body also includes a cover body for covering the opening of the concave area, and the cover body is hinged to the box body.

2. The microfluidic device for detecting drainage fluid amylase according to claim 1, characterized in that: The box body comprises a base and a support frame, the base is provided with a slot for longitudinally inserting the support frame, and the support frame is plugged into the base; The support frame and the base are fixed to each other by locking screws; The microfluidic chip is installed on the supporting frame.

3. The microfluidic device for detecting drainage fluid amylase according to claim 2, characterized in that: The base is provided with a boss, the support frame is provided with a support plate for overlapping the boss, the support plate is provided with a notch, and the boss is provided with a positioning protrusion embedded in the notch; The locking screw is threadedly connected to the positioning protrusion, and the locking screw abuts against the supporting plate.

4. The microfluidic device for detecting drainage fluid amylase according to claim 2, characterized in that: The outer side of the base is provided with a placement groove for placing the battery, and the notch of the placement groove is slidably connected to a cover for sealing the notch.

5. The microfluidic device for detecting drainage fluid amylase according to claim 2, characterized in that: The support frame is provided with openings for exposing the reaction area and the color development area; A heating plate for heating the reaction zone is installed on the base, and the heating plate is located directly below the reaction zone.

6. The microfluidic device for detecting drainage fluid amylase according to claim 5, characterized in that: A photosensitive sensor and a light source are respectively installed on the upper and lower sides of the color development area. The photosensitive sensor is installed on the cover body, and the light source is installed on the base.

7. The microfluidic device for detecting drainage fluid amylase according to claim 1, characterized in that: The first introduction tube is connected to a drainage tube extending out of the shell, and the shell is provided with a through hole for the drainage tube to pass through; The second introduction tube is connected to a starch solution storage bag through a hose; The third introduction tube is connected to an iodine solution storage bag through a hose.

8. The microfluidic device for detecting drainage fluid amylase according to claim 1, characterized in that: The reaction channel is a circuitous serpentine pipeline, and the color development channel is a circuitous serpentine pipeline; The first inlet pipe, the third inlet pipe and the outlet pipe are arranged in sequence along a first straight line direction; The first introduction tube and the second introduction tube are arranged along a second straight line direction; The first straight line direction is perpendicular to the second straight line direction.

9. The microfluidic device for detecting drainage fluid amylase according to claim 1, characterized in that: The cover body is detachably connected to the box body through a locking mechanism.

Images