Rapid detection device for rapid biological indicator

Abstract

The utility model provides a quick detection device for quick type biological indicators, which comprises a shell, a darkroom and a control system, the darkroom and the control system are arranged in the shell, a camera component and a culture room corresponding to the camera component are arranged in the darkroom, a plurality of detection holes for placing the biological indicators are arranged in the culture room, and the detection holes are communicated with the control system. An ultraviolet light source is arranged in the detection hole, a mounting hole corresponding to the detection hole is formed in the cover plate above the darkroom, and an opening is formed in the position, corresponding to the mounting hole, of the shell. According to the utility model, the super-definition starlight CCD camera is adopted to detect fluorescence generated in the biological indicator, the detection speed is high, the accuracy is high, and real-time monitoring and alarming can be realized.

Description

Technical Field

[0001] The utility model relates to the technical field of microbial detection, in particular to a rapid detection device for rapid biological indicators. Background Art

[0002] Traditional biological indicators require 24-48 hours of culture to produce results, which cannot meet the requirements of immediate recycling of many sterilized items (medical devices, etc.). The thermophilic Bacillus contained in the rapid biological indicator can produce and secrete glycosidase substances during the growth process. This type of enzyme metabolite can decompose 4-methylumbelliferyl-α-D-glucoside or 4-methylumbelliferyl-β-D-glucoside in the selective culture medium to produce 4-methylumbelliferyl (abbreviated as 4-MU) with blue fluorescence at a wavelength of 366nm. The survival of spores in the rapid biological indicator is usually determined by detecting whether the fluorescence in the culture system is excited, so as to achieve rapid detection.

[0003] Traditional rapid detection methods usually use photoelectric sensors such as silicon photocells to detect the weak fluorescence stimulated by the metabolic products produced by microorganisms during their growth. This rapid detection method is easily interfered by strong external light, which can lead to misjudgment of the test results and false positive or false negative results. At the same time, since the content of spores that have not been killed is small, most of them are damaged during the sterilization process, which slows down the growth of spores and produces extremely weak fluorescence, which is difficult to detect with traditional detection methods. Summary of the invention

[0004] In order to solve the defects of the above-mentioned prior art, the utility model provides a rapid detection device for rapid biological indicators, which can detect the weaker fluorescent substances produced by spores, has fast detection speed, high accuracy, and can monitor and alarm in real time.

[0005] The technical solutions adopted are as follows:

[0006] A rapid detection device for rapid biological indicators comprises a shell, a darkroom arranged in the shell and a control system, wherein the darkroom is provided with a camera assembly and a culture chamber corresponding to the camera assembly, wherein the culture chamber is provided with a plurality of detection holes for placing biological indicators, wherein the detection holes are provided with ultraviolet light sources, wherein a cover plate above the darkroom is provided with mounting holes corresponding to the detection holes, and wherein openings are provided at positions of the shell corresponding to the mounting holes.

[0007] Furthermore, the camera assembly includes an industrial camera, a wide-angle lens, and a mounting assembly for fixing the camera and the lens. The wide-angle lens is provided with a filter that can filter out light other than fluorescence on the side facing the culture chamber. The industrial camera is an ultra-clear starlight CCD camera. Furthermore, the mounting assembly includes a mounting bracket provided on a side wall of the darkroom, and the CCD camera is mounted on the mounting bracket through a nylon spacer.

[0008] Furthermore, the control system includes an AI image processing module that performs special processing on photos after receiving photos transmitted by the camera component, an alarm module that will alarm on the screen when fluorescence is detected, and a transmission module that transmits information to a cloud server.

[0009] Furthermore, the culture chamber is in an arc shape corresponding to the field of view of the CCD camera.

[0010] Furthermore, a human-computer interaction screen is provided on the housing.

[0011] Furthermore, a micro printer is provided at the lower part of the shell.

[0012] Compared with the prior art, the beneficial effects produced by the utility model are:

[0013] The utility model provides a rapid detection device for rapid biological indicators, including a darkroom, a culture chamber for storing biological indicators, and a camera assembly for recording whether the culture chamber produces fluorescence. The camera assembly includes an ultra-clear starlight CCD camera with extremely low illumination and a wide-angle lens. The camera can capture weak fluorescence in a dark environment, and judge whether fluorescence is generated through image processing of the control system. If fluorescence is generated, an alarm is sounded, and data information can be uploaded to a cloud server. The CCD camera captures and detects fluorescence with high accuracy and speed, and can be coordinated with the control system for real-time monitoring and data recording. It meets the requirements of instant reuse of various sterilization equipment. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0015] Figure 2 It is a disassembly diagram of the utility model;

[0016] Figure 3 is a side view of the camera assembly;

[0017] Figure 4 is the field of view of the camera assembly;

[0018] Among them, housing 1, darkroom 2, camera assembly 3, industrial camera 301, wide-angle lens 302, filter 303, mounting bracket 304, nylon spacer 305, culture chamber 4, biological indicator 401, detection hole 402, screen 5, micro printer 6, mounting hole 7, opening 8. DETAILED DESCRIPTION

[0019] The present invention will be further described below in conjunction with specific embodiments.

[0020] refer to Figure 1 , 2 , 3, 4, a rapid detection device for rapid biological indicators, comprising a shell 1, a darkroom 2 arranged in the shell and a control system, the darkroom 2 is provided with a camera assembly 3 and a culture chamber 4 corresponding to the camera assembly, the culture chamber 4 is provided with a plurality of detection holes 402 for placing biological indicators 401.

[0021] Furthermore, the darkroom is set up to prevent interference from external ambient light sources and make the fluorescent effect more obvious.

[0022] Furthermore, the fluorescent substance produced by the microbial spores in the biological indicator 401 is 4-methylumbelliferone, which can produce blue fluorescence under the irradiation of ultraviolet light.

[0023] Furthermore, an ultraviolet light source is provided in the detection hole 402 to excite the fluorescent substance produced by the microbial spores in the biological indicator 401 to produce fluorescence.

[0024] As a further preference, a 366nm short-wave pass filter is arranged in front of the ultraviolet light source to filter out light of about 465nm that may be generated by the ultraviolet light source, thereby preventing the external environment from interfering with the detection results.

[0025] Furthermore, a mounting hole 7 corresponding to the detection hole 402 is provided on the cover plate above the darkroom 2, and an opening 8 is provided at a position of the housing 1 corresponding to the mounting hole 7. The detection chamber 4 is arranged in the darkroom 2, and the mounting hole 7 is arranged at the position of the detection hole, which neither affects the detection of fluorescence nor facilitates the placement of the biological indicator 401. The corresponding biological indicator can be directly placed in the detection hole 402.

[0026] Furthermore, the camera assembly 3 includes an industrial camera 301, a wide-angle lens 302, and a mounting assembly for fixing the camera and the lens. The industrial camera is preferably an ultra-clear starlight CCD camera. The ultra-clear starlight CCD camera has ultra-clear extremely low illumination and is used to detect whether the fluorescent substance in the biological indicator is excited to determine whether the spores are alive.

[0027] Furthermore, the mounting assembly includes a mounting bracket 304 disposed on a side wall of the darkroom, and the industrial camera 301 is mounted on the mounting bracket 304 via a nylon spacer 305 .

[0028] Furthermore, the industrial camera 301 has high resolution, low output signal noise, and stronger anti-interference ability. It can take ultra-clear photos under extremely low illumination conditions and can identify weak fluorescence. Even when the spore content is very small, it can be detected, and the detection result is more accurate.

[0029] Furthermore, providing a wide-angle lens 302 can expand the shooting range.

[0030] Furthermore, a filter 303 that can filter out light other than fluorescence is provided on the side of the wide-angle lens 302 facing the culture chamber. This enables the camera to capture specific fluorescence more accurately without being disturbed by other light sources. The filter 303 is a 465nm blue light filter, which ensures that only light around 465nm enters the camera's photosensitive system, allowing the photosensitive system to accurately sense the fluorescence emitted by the fluorescent substance.

[0031] Furthermore, the camera component 3 intermittently takes pictures of the fluorescent image, and can take pictures intermittently during the culture process to monitor the process in real time.

[0032] Furthermore, the housing 1 is provided with a screen 5 for human-computer interaction.

[0033] Furthermore, the control system includes an AI image processing module that performs special processing on the photos after receiving the photos transmitted by the camera component, an alarm module that detects fluorescence and issues an alarm on the screen 5, and a transmission module that transmits the information to the cloud server.

[0034] Furthermore, the control system adopts a high-performance embedded central processor and uses an intelligent AI image processing algorithm to process and analyze the images captured by the ultra-clear Starlight Industrial Camera 301. The images in the algorithm are stored in the HSV color space that is closer to the human eye, and the images are filtered and screened by the H (hue), S (saturation), and V (brightness) parameters corresponding to 465nm light. The noise is then filtered out by image processing algorithms such as Gaussian filtering and morphological processing. Finally, the image is binarized and then used for threshold screening to finally determine whether the metabolite produces specific fluorescence under the excitation of a specific ultraviolet light source.

[0035] Furthermore, the transmission module adopts a 4G transmission module, and cooperates with a cloud server to share detection information in real time. When the specific fluorescence produced by the metabolite is detected in the image information captured by the industrial camera 301, the instrument will issue an alarm message and upload it to the cloud server, and then distribute it to each mobile terminal App by the lower-level control system, realizing unmanned laboratory testing, without the need for on-site monitoring, and instantly transmitting the test results, meeting the requirements for instant reuse of various sterilization equipment.

[0036] Furthermore, a micro printer 6 is provided at the lower part of the housing 1 for printing a test report.

[0037] Furthermore, the culture chamber 4 is in an arc shape corresponding to the field of view of the CCD camera. The arc is consistent with the field of view of the CCD camera, which reduces the distortion of the photograph.

[0038] The utility model can be used to detect whether the sterilization is thorough. The metabolic products produced by the microorganisms in the rapid biological indicator 401 during the culture process can emit blue fluorescence under the irradiation of the ultraviolet light source. The biological indicator is cultured in the culture chamber 4. If spores survive, the biological indicator will emit fluorescence after being irradiated by the ultraviolet light source. The industrial camera 301 will record the photo of the indicator in real time and transmit it to the control system for image processing. The processed image is observed to see whether fluorescence appears. If fluorescence appears, the screen 5 will alarm, indicating that spores are still alive and the sterilization is not thorough enough.

[0039] The above is only a specific implementation of the utility model, but the protection scope of the utility model is not limited thereto. Any changes or substitutions that can be easily thought of by a person skilled in the art within the technical scope disclosed by the utility model should be included in the protection scope of the utility model. Therefore, the protection scope of the utility model should be based on the protection scope of the claims.

Claims

1. A rapid detection device for rapid biological indicators, characterized in that: The invention comprises a housing (1), a darkroom (2) arranged in the housing, and a control system; the darkroom (2) is provided with a camera assembly (3) and a culture chamber (4) corresponding to the camera assembly; the culture chamber (4) is provided with a plurality of detection holes (402) for placing biological indicators (401); the detection holes (402) are provided with ultraviolet light sources; a cover plate above the darkroom (2) is provided with mounting holes (7) corresponding to the detection holes (402); and the housing (1) is provided with openings (8) at locations corresponding to the mounting holes (7).

2. The rapid detection device for rapid biological indicators according to claim 1, characterized in that: The camera assembly (3) comprises an industrial camera (301), a wide-angle lens (302), and a mounting assembly for fixing the camera and the lens. The wide-angle lens (302) is provided with a filter (303) capable of filtering out light other than fluorescence on the side facing the culture chamber.

3. The rapid detection device for rapid biological indicators according to claim 2, characterized in that: The mounting assembly comprises a mounting bracket (304) arranged on a side wall of the darkroom, and the industrial camera (301) is mounted on the mounting bracket (304) via a nylon spacer (305).

4. The rapid detection device for rapid biological indicators according to claim 1, characterized in that: The control system comprises an AI image processing module for processing photos after receiving photos transmitted by the camera component (3), an alarm module for alarming, and a transmission module for transmitting information to a cloud server.

5. The rapid detection device for rapid biological indicators according to claim 2, characterized in that: The culture chamber (4) is in an arc shape corresponding to the field of view of the industrial camera.

6. The rapid detection device for rapid biological indicators according to claim 2, characterized in that: The industrial camera is an ultra-clear starlight CCD.

7. The rapid detection device for rapid biological indicators according to claim 1, characterized in that: The housing (1) is provided with a human-computer interaction screen (5).

8. The rapid detection device for rapid biological indicators according to claim 1, characterized in that: A micro printer (6) is provided at the lower part of the housing (1).

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