Beauveria bassiana rapid screening device based on DNA bar codes

Abstract

The utility model discloses a rapid beauveria bassiana screening device with a DNA bar code, which is characterized in that through the arrangement of an observation table, an electron microscope, a display screen and other structures, when samples on a glass slide need to be screened, the samples of the beauveria bassiana with the DNA bar code are placed on the observation table, and the samples on the glass slide are screened by the observation table; the observation table is sequentially rotated to drive the glass slide to move to the right lower end of the electron microscope, a sample on the glass slide is amplified and observed, and observed data are transmitted to the display screen in real time, so that the sample on the glass slide can be conveniently and visually observed and screened, and meanwhile, by means of the self-locking performance of the worm and the worm gear, when the observation table rotates, the sample on the glass slide can be conveniently and visually observed and screened. According to the device, the glass slides can rotate in the single direction only, so that the situation that samples on the glass slides are repeatedly observed is avoided, different samples can be conveniently and rapidly marked through the arrangement of the electric telescopic rod and the marking pen, different glass slides can be visually and rapidly distinguished after detection is finished, and confusion is avoided.

Description

Technical Field

[0001] The utility model relates to the technical field of screening devices, in particular to a DNA barcode Beauveria bassiana rapid screening device. Background Art

[0002] Beauveria bassiana is an entomogenous fungus of the Ascomycetes family. The main species include Beauveria bassiana and Beauveria brandoni. It often reproduces asexually to produce conidia. Beauveria bassiana can invade the bodies of more than 200 species of insects and mites from 15 families in six orders and reproduce in large numbers. It also produces beauvericin (a non-ribosomal polypeptide toxin), oosporin (a benzoquinone toxin) and calcium oxalate crystals. These substances can cause poisoning in insects, disrupt metabolism and lead to death.

[0003] In the prior art, when screening DNA barcoded Beauveria bassiana, the traditional screening method is to directly place a glass slide with fluorescent staining on an observation table and then observe and screen. However, this screening method not only requires the staff to place the glass slides with Beauveria bassiana on the observation table one by one for observation, but also easily leads to sample confusion when there are many samples, and the time-consuming observation using a microscope equipment can easily cause eye fatigue.

[0004] Therefore, a DNA barcode Beauveria bassiana rapid screening device is proposed. Utility Model Content

[0005] The utility model aims to provide a DNA barcode Beauveria bassiana rapid screening device to solve the problems raised in the above background technology.

[0006] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a DNA barcoded Beauveria bassiana rapid screening device, comprising a workbench, a plurality of glass slides are arranged on the workbench, an observation screening mechanism is arranged on the workbench, the observation screening mechanism is mounted on the glass slide, a rotating mechanism, a retaining mechanism and a marking mechanism are mounted on the observation screening mechanism, the observation screening mechanism comprises a bearing, the bearing is mounted on the workbench, a rotating shaft is rotatably mounted on the bearing, an observation platform is mounted on the rotating shaft, a plurality of mounting grooves are equidistantly arranged in a circle on the observation platform, glass slides are arranged in the plurality of mounting grooves, an L-shaped rod is mounted on the workbench, an electron microscope is mounted on the bottom side of the L-shaped rod, and the electron microscope is adapted to the glass slide.

[0007] Preferably, a display screen is installed on the L-shaped rod, and the display screen is electrically connected to the electron microscope via a cable.

[0008] Preferably, the rotating mechanism comprises two fixed blocks, both of which are mounted on a workbench, a worm is rotatably mounted on the two fixed blocks, a worm wheel is meshed on the worm, and the worm wheel is sleeved on the rotating shaft.

[0009] Preferably, a support plate is mounted on the fixed block located on one side, a servo motor is mounted on the support plate, a drive shaft is mounted on the servo motor, and the drive shaft is mounted on one side of the worm.

[0010] Preferably, the retaining mechanism includes two sliding holes, and the observation platform is provided with two sliding holes, guide rods are slidably installed on the inner walls of the two sliding holes, and retaining frames are installed on the top ends of the two guide rods, and the bottom side of the retaining frame is in contact with the slide.

[0011] Preferably, a return spring is sleeved on the guide rod, one end of the return spring is mounted on the bottom side of the retaining frame, and the other end of the return spring is mounted on the bottom inner wall of the sliding hole.

[0012] Preferably, the marking mechanism comprises an electric telescopic rod, which is mounted on the L-shaped rod, and a marking pen is mounted on the bottom side of the electric telescopic rod, and the marking pen is in contact with the retaining frame.

[0013] Compared with the prior art, the beneficial effects of the utility model are:

[0014] (1) The utility model, through the arrangement of structures such as an observation platform, an electron microscope, a display screen, a worm gear, a worm and a servo motor, when it is necessary to screen the sample on the glass slide, the sample of Beauveria bassiana equipped with a DNA barcode is placed on the observation platform, and then the observation platform is rotated in sequence to drive the glass slide to move to the lower end of the electron microscope, and the sample on the glass slide is magnified and observed, and the observed data is transmitted to the display screen in real time, so that the sample on the glass slide can be observed and screened conveniently and intuitively. At the same time, the self-locking property of the worm and the worm gear is utilized so that the observation platform can only rotate in one direction when rotating, and will not rotate in the opposite direction, so that the sample on the glass slide will not be observed repeatedly. Through the arrangement of the electric telescopic rod and the marking pen, unqualified samples can be quickly and conveniently marked, so that different glass slides can be quickly and intuitively distinguished after the test is completed to avoid confusion.

[0015] (2) The utility model, through the arrangement of the guide rod, the retaining frame and the reset spring and other structures, can conveniently use the retaining frame to fix the glass slide on the observation platform by utilizing the restoring force of the reset spring, thereby preventing the position of the glass slide from shifting due to the observation platform rotating too fast during the rotation of the observation platform, thereby preventing the glass slide from being normally observed using an electron microscope. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a schematic diagram of the main structure of the utility model;

[0017] Figure 2 It is a side view structural schematic diagram of the utility model;

[0018] Figure 3 It is a schematic diagram of the connection structure of the retention frame, the electron microscope and the marking pen of the utility model;

[0019] Figure 4 It is a cross-sectional structural schematic diagram of the observation platform and the retention frame of the utility model;

[0020] In the figure: 1. workbench; 2. slide; 3. bearing; 31. rotating shaft; 32. observation platform; 33. mounting slot; 34. L-shaped rod; 35. electron microscope; 36. display screen; 4. fixing block; 41. worm; 42. worm gear; 43. driving shaft; 44. servo motor; 45. support plate; 5. sliding hole; 51. guide rod; 52. retaining frame; 53. reset spring; 6. electric telescopic rod; 61. marking pen. DETAILED DESCRIPTION

[0021] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0022] Example: See Figure 1-4The utility model provides a technical solution: a DNA barcode Beauveria bassiana rapid screening device, comprising a workbench 1, a plurality of glass slides 2 are arranged on the workbench 1, an observation screening mechanism is arranged on the workbench 1, the observation screening mechanism is mounted on the glass slide 2, a rotating mechanism, a retaining mechanism and a marking mechanism are mounted on the observation screening mechanism, in order to facilitate intuitive and clear observation of the image on the glass slide 2, an observation screening mechanism is arranged on the workbench 1, specifically comprising a bearing 3, the bearing 3 is mounted on the workbench 1, A rotating shaft 31 is rotatably mounted on the bearing 3, an observation platform 32 is mounted on the rotating shaft 31, a plurality of mounting grooves 33 are equidistantly arranged in an annular pattern on the observation platform 32, a glass slide 2 is arranged in each of the plurality of mounting grooves 33, an L-shaped rod 34 is mounted on the workbench 1, an electron microscope 35 is mounted on the bottom side of the L-shaped rod 34, the electron microscope 35 is adapted to the glass slide 2, a display screen 36 is mounted on the L-shaped rod 34, the display screen 36 is electrically connected to the electron microscope 35 through a cable, and the rotating mechanism includes two fixed blocks 4, two The fixed blocks 4 are all installed on the workbench 1. A worm 41 is rotatably installed on the two fixed blocks 4. A worm wheel 42 is meshed on the worm 41. The worm wheel 42 is sleeved on the rotating shaft 31. A support plate 45 is installed on the fixed block 4 on one side. A servo motor 44 is installed on the support plate 45. A drive shaft 43 is installed on the servo motor 44. The drive shaft 43 is installed on one side of the worm 41. The servo motor 44 drives the drive shaft 43 to rotate, and the drive shaft 43 drives the worm 41 to rotate. The worm 41 drives the rotating shaft 31. The worm gear 42 rotates, and the worm gear 42 drives the rotating shaft 31 to rotate on the bearing 3, and the rotating shaft 31 drives the observation platform 32 to rotate, so that the glass slide 2 can be conveniently moved in one direction in sequence to the bottom of the electron microscope 35 for observation. When the electron microscope 35 observes the image on the glass slide 2 directly below, it will be transmitted to the display screen 36 on the L-shaped rod 34 in real time through the cable, and the image on the glass slide 2 will be enlarged by the display screen 36, so that the staff can observe the image on the display screen 36 conveniently.

[0023] In order to conveniently fix the glass slide 2 on the observation platform 32 to avoid displacement during the movement, a fixing mechanism is arranged on the observation platform 32, specifically including two sliding holes 5. The observation platform 32 is provided with two sliding holes 5. Guide rods 51 are slidably installed on the inner walls of the two sliding holes 5. A fixing frame 52 is installed on the top of the two guide rods 51. The bottom side of the fixing frame 52 contacts the glass slide 2. A return spring 53 is sleeved on the guide rod 51. One end of the return spring 53 is installed on the bottom side of the fixing frame 52. The other end of the return spring 53 is installed on the inner wall of the bottom side of the sliding hole 5. The fixing frame 52 is lifted upward, and the fixing frame 52 drives the return spring 53 on the guide rod 51 to stretch. Then the glass slide 2 is placed in the mounting groove 33 on the observation platform 32. At this time, the fixing frame 52 is released, and the restoring force of the return spring 53 is used to drive the fixing frame 52 to fix the glass slide 2.

[0024] In order to be able to quickly distinguish different glass slides 2 during the detection process, a marking mechanism is arranged on the L-shaped rod 34, specifically including an electric telescopic rod 6, which is installed on the L-shaped rod 34. A marking pen 61 is installed on the bottom side of the electric telescopic rod 6, and the marking pen 61 is in contact with the retaining frame 52. When an abnormal image is observed, the switch on the electric telescopic rod 6 can be driven, and the electric telescopic rod 6 can be used to drive the marking pen 61 to move downward to make a mark on the retaining frame 52. Therefore, after a group of samples are observed, the staff can find different samples intuitively and quickly, which is convenient for the staff to screen and sort.

[0025] The working principle is as follows: when screening the sample on the slide 2, lift the retaining frame 52 upward, the retaining frame 52 drives the reset spring 53 on the guide rod 51 to stretch, and then place the slide 2 in the mounting groove 33 on the observation platform 32, then release the retaining frame 52, use the restoring force of the reset spring 53 to drive the retaining frame 52 to fix the slide 2, and then start the switch of the servo motor 44 on the support plate 45, use the servo motor 44 to drive the drive shaft 43 to rotate, the drive shaft 43 drives the worm 41 to rotate, the worm 41 drives the worm wheel 42 on the rotating shaft 31 to rotate, the worm wheel 42 drives the rotating shaft 31 to rotate on the bearing 3, and the rotating shaft 31 drives the observation platform 32 to rotate, so that the slide 2 can be conveniently moved in one direction in sequence. The glass slide 2 is moved to the bottom of the electron microscope 35 for observation. When the electron microscope 35 observes the image on the glass slide 2 below, it will be transmitted to the display screen 36 on the L-shaped rod 34 in real time through the cable. The display screen 36 is used to magnify the image on the glass slide 2, so that the staff can observe the image on the display screen 36. When the staff observes the image displayed on the display screen 36 and observes an abnormal image, they can drive the switch on the electric telescopic rod 6 and use the electric telescopic rod 6 to drive the marking pen 61 to move downward to make a mark on the retaining frame 52. After a group of samples are observed, the staff can find different samples intuitively and quickly, which is convenient for the staff to screen and sort.

[0026] Although the embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A DNA barcode Beauveria bassiana rapid screening device, characterized in that: The invention comprises a workbench (1), wherein a plurality of glass slides (2) are arranged on the workbench (1), an observation and screening mechanism is arranged on the workbench (1), the observation and screening mechanism is mounted on the glass slides (2), a rotation mechanism, a fixing mechanism and a marking mechanism are mounted on the observation and screening mechanism, the observation and screening mechanism comprises a bearing (3), the bearing (3) is mounted on the workbench (1), a rotating shaft (31) is rotatably mounted on the bearing (3), an observation platform (32) is mounted on the rotating shaft (31), a plurality of mounting grooves (33) are equidistantly arranged in an annular manner on the observation platform (32), the plurality of mounting grooves (33) are each provided with a glass slide (2), an L-shaped rod (34) is mounted on the workbench (1), an electron microscope (35) is mounted on the bottom side of the L-shaped rod (34), and the electron microscope (35) is adapted to the glass slide (2).

2. The DNA barcode Beauveria rapid screening device according to claim 1, characterized in that: A display screen (36) is installed on the L-shaped rod (34), and the display screen (36) is electrically connected to the electron microscope (35) via a cable.

3. The DNA barcode Beauveria rapid screening device according to claim 1, characterized in that: The rotating mechanism comprises two fixed blocks (4), both of which are mounted on the workbench (1), a worm (41) being rotatably mounted on the two fixed blocks (4), a worm wheel (42) being meshed on the worm (41), and the worm wheel (42) being sleeved on the rotating shaft (31).

4. The DNA barcode Beauveria rapid screening device according to claim 3, characterized in that: A support plate (45) is installed on the fixed block (4) located on one side, a servo motor (44) is installed on the support plate (45), a drive shaft (43) is installed on the servo motor (44), and the drive shaft (43) is installed on one side of the worm (41).

5. The DNA barcode Beauveria rapid screening device according to claim 1, characterized in that: The retaining mechanism comprises two sliding holes (5). The observation platform (32) is provided with two sliding holes (5). Guide rods (51) are slidably mounted on the inner walls of the two sliding holes (5). A retaining frame (52) is mounted on the top ends of the two guide rods (51). The bottom side of the retaining frame (52) is in contact with the glass slide (2).

6. The DNA barcode Beauveria rapid screening device according to claim 5, characterized in that: A return spring (53) is sleeved on the guide rod (51), one end of the return spring (53) is mounted on the bottom side of the retaining frame (52), and the other end of the return spring (53) is mounted on the bottom inner wall of the sliding hole (5).

7. The DNA barcode Beauveria rapid screening device according to claim 5, characterized in that: The marking mechanism comprises an electric telescopic rod (6), which is mounted on an L-shaped rod (34). A marking pen (61) is mounted on the bottom side of the electric telescopic rod (6), and the marking pen (61) is in contact with a retaining frame (52).

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