Antibody incubation box applied to western blot method

Abstract

The antibody incubation box comprises a box body, a containing container is arranged in the box body in a clearance fit mode, a plurality of arc-shaped incubation containing grooves and cold storage material containing grooves are formed in the containing container at intervals, a box cover is arranged at the upper end of the box body, and a box cover opening and closing plate is rotationally connected to the box cover through a notch. A guide cylinder is arranged in the middle of the box cover, a pressing rotating structure is arranged in the guide cylinder, and after the pressing rotating structure is pressed, the pressing rotating structure sequentially drives the arc incubation containing grooves and the cold storage material containing grooves to move to the notches. According to the antibody incubation box, the box cover is tightly attached to the rubber pad at the top end of the containing container under the action of the third spring to form a sealing structure, so that an antibody solution in the arc-shaped incubation placing groove is not easy to spill and mix when the antibody incubation box violently shakes or inverts; the purpose of reducing pollution to the membrane or the antibody can be achieved through single exposure of the arc-shaped incubation placing groove, and evaporation of the antibody is reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of biological experiments, in particular to an antibody incubation box used for protein blotting. Background Art

[0002] Western Blot is a commonly used experimental method in molecular biology, biochemistry and immunogenetics. It is an experimental method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Antibody incubation boxes are widely used in blocking, membrane washing, primary antibody incubation and secondary antibody incubation in Western blotting experiments. Common antibody incubation boxes have a fixed-size multi-grid box slot inside, and the whole is a light-transmitting square box formed by the hinged box cover and box body.

[0003] The antibody incubation boxes currently in use have the following defects: the entire box cover needs to be opened each time the PVDF membrane or NC membrane in the tank is replaced, and repeated contact between the antibody and the air cannot be avoided. When the box cover is opened, all antibody incubation tanks are exposed at the same time, which is easy to cause contamination and increase antibody evaporation. Utility Model Content

[0004] The purpose of the utility model is to improve and innovate the shortcomings and problems existing in the background technology, and to provide an antibody incubation box applied to protein blotting.

[0005] An antibody incubation box used for protein blotting comprises a box body, a containing container is gap-fitted inside the box body, a plurality of arc-shaped incubation placement grooves and cold storage material placement grooves are arranged at intervals on the containing container, a box cover is arranged at the upper end of the box body, a switch box cover plate is rotatably connected to the box cover via a notch, a guide cylinder is arranged in the middle of the box cover, a push-to-rotate structure is arranged inside the guide cylinder, and after pressing the push-to-rotate structure, the push-to-rotate structure will sequentially drive each arc-shaped incubation placement groove and cold storage material placement groove to move to the notch.

[0006] A further solution is that the push-rotation structure includes a button and a knob that slide with each other, and the button is slidably arranged in the guide cylinder, the knob is installed in the middle of the bottom wall of the holding container, a first spring is arranged between the button and the guide cylinder, and a plurality of vertical grooves are distributed in a circular array on the outer wall of the knob, and the top and bottom ends of adjacent vertical grooves are connected to each other through an oblique groove; a wedge block is installed in the vertical groove, and the wedge block includes an inclined surface corresponding to the bottom end of the oblique groove and a table surface corresponding to the top end of the oblique groove, a groove is opened on the inner wall of the button, and a second spring is fixedly connected to the bottom wall of the groove, and a pin is arranged at the end of the second spring away from the bottom wall of the groove, and the pin is adapted to the vertical groove and the oblique groove.

[0007] A further solution is that an annular protrusion is provided on the outer surface of the button, a guide groove matched with the annular protrusion is opened on the guide cylinder, the first spring is wound around the outer surface of the button, and both ends of the first spring are fixedly connected to the annular protrusion and the bottom wall of the guide groove.

[0008] A further solution is that the height of the inclined groove gradually increases from the top to the bottom, so that a first step is formed between the bottom of the vertical groove and the inclined groove, and a second step is formed between the top of the vertical groove and the inclined groove.

[0009] A further solution is that the bottom end of the containing container is rotatably connected to a lifting plate, a third spring is arranged between the lifting plate and the bottom wall of the box body, a rubber pad is arranged at the top of the containing container, a handle is arranged at one end of the lifting plate, and the handle is slidably matched with the limiting groove on the side wall of the box body.

[0010] A further solution is that a ball bearing is arranged between the bottom wall of the containing container and the lifting plate.

[0011] A further solution is that a liquid guide tube is threadedly connected to the box body and the containing container through an initial threaded hole, a plug is inserted at the end of the liquid guide tube, and the liquid guide tube is connected to the arc-shaped incubation placement groove.

[0012] A further solution is that the number of the arc-shaped incubation placement grooves and the cold storage material placement grooves is set to eight.

[0013] Compared with the prior art, the utility model has the following beneficial effects: the box cover of the utility model is tightly fitted with the rubber pad on the top of the container under the action of the third spring to form a sealing structure, so that the antibody solution in the arc-shaped incubation placement groove is not easy to spill or mix when the antibody incubation box is violently shaken or turned over; the liquid guide tube on the front wall of the arc-shaped incubation placement groove is convenient for adding, recycling or discarding the antibody solution, and effectively avoids the outflow of PVDF membrane or NC membrane during the liquid addition process; in addition, adding liquid through the liquid guide tube can prevent the impact force on the PVDF membrane or NC membrane from being too large during the liquid addition, affecting the protein on the membrane; when placing the PVDF membrane or NC membrane in the incubation placement groove, the single exposure of the arc-shaped incubation placement groove can achieve the purpose of reducing the contamination of the membrane or antibody and reducing the evaporation of the antibody. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] In order to more clearly illustrate the technical solutions of the embodiments of the utility model, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0015] Figure 1A schematic cross-sectional structure diagram of an antibody incubation box for use in a Western blotting method provided by an embodiment of the utility model;

[0016] Figure 2 A schematic diagram of a top view of an antibody incubation box for use in a Western blotting method provided by an embodiment of the utility model;

[0017] Figure 3 The embodiment of the utility model provides Figure 1 A schematic diagram of the partially enlarged structure at center A;

[0018] Figure 4 This is a schematic cross-sectional structure diagram of a push-to-rotate structure provided in an embodiment of the utility model.

[0019] Figure numerals: box body 1, box cover 2, guide cylinder 3, guide groove 31, push-rotate structure 4, button 41, annular protrusion 411, first spring 412, groove 413, second spring 414, pin 415, knob 42, vertical groove 421, inclined groove 422, inclined surface 4231, table top 4232, wedge block 423, first step 424, second step 425, holding container 5, arc-shaped incubation placement groove 51, cold storage material placement groove 52, notch 6, switch box cover 7, block 8, rubber pad 9, liquid guide tube 10, plug 11, handle 12, lifting plate 13, third spring 14. DETAILED DESCRIPTION

[0020] In order to make the purpose, features and advantages of the present invention more obvious and easy to understand, the specific implementation methods of the present invention are described in detail below with reference to the accompanying drawings.

[0021] It should be noted that when an element is referred to as being "fixed to" another element, it may be directly on the other element or there may be an intermediate element. When an element is considered to be "connected to" another element, it may be directly connected to the other element or there may be an intermediate element at the same time.

[0022] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art in the technical field of the present invention. The terms used herein in the specification of the present invention are only for the purpose of describing specific implementations and are not intended to limit the present invention. The term "and / or" used herein includes any and all combinations of one or more related listed items.

[0023] See also Figure 1-Figure 4The utility model provides an antibody incubation box for protein blotting, comprising a box body 1, wherein a containing container 5 is provided in the internal gap of the box body 1, and a plurality of arc-shaped incubation placement grooves 51 and cold storage material placement grooves 52 are arranged at intervals on the containing container 5. The number of the arc-shaped incubation placement grooves 51 and the cold storage material placement grooves 52 is set to eight, and a PVDF membrane or an NC membrane and an antibody solution are added to the arc-shaped incubation placement groove 51; and a cold storage material is added to the cold storage material placement groove 52. The cold storage material can be an ice crystal gel, and the cold storage material is used to keep the antibody solution in the arc-shaped incubation placement groove 51 at a low temperature. Since the cold storage material can be recycled; therefore, in this embodiment, the arc-shaped incubation placement groove 51 can be set to an upper open structure, and the cold storage material placement groove 52 can be set to an upper closed structure.

[0024] See also Figure 1 and Figure 2 The upper end of the box body 1 is provided with a box cover 2, and a notch 6 is provided on the box cover 2. A switch box cover plate 7 is rotatably connected to the notch 6. In this embodiment, the box cover 2 is rotatably connected to the switch box cover plate 7 through a hinge. A clamping block 8 is provided on the switch box cover plate 7. The clamping block 8 cooperates with the buckle on the box body 1, so that the switch box cover plate 7 is fixed on the box body 1. The size of the switch box cover plate 7 is adapted to the size of the two arc-shaped incubation placement grooves 51 and the one cold storage material placement groove 52, or the size of the switch box cover plate 7 is adapted to the size of the one arc-shaped incubation placement groove 51 and the one cold storage material placement groove 52. A guide cylinder 3 is provided in the middle of the box cover 2, and a push-rotate structure 4 is provided inside the guide cylinder 3. After pressing the push-rotate structure 4, the push-rotate structure 4 will drive each arc-shaped incubation placement groove 51 and the cold storage material placement groove 52 to move to the gap 6 in turn; after opening the gap 6, it is convenient to replace the VDF membrane or NC membrane into each arc-shaped incubation placement groove 51, so that when placing the PVDF membrane or NC membrane in the arc-shaped incubation placement groove 51, the single exposure of the arc-shaped incubation placement groove 51 can achieve the purpose of reducing the contamination of the membrane or antibody, and reduce the evaporation of the antibody solution.

[0025] See 1 and Figure 3, the push-to-rotate structure 4 includes a button 41 and a knob 42 that slide with each other, and the button 41 is slidably arranged in the guide cylinder 3. The knob 42 is installed in the middle of the bottom wall of the containing container 5, and a first spring 412 is arranged between the button 41 and the guide cylinder 3. A plurality of vertical grooves 421 are distributed in a circular array on the outer wall of the knob 42, and the number of the vertical grooves 421 corresponds to the number of the arc-shaped incubation placement grooves 51; therefore, in this embodiment, the number of vertical grooves 421 is eight. The top and bottom ends of adjacent vertical grooves 421 are connected to each other through an inclined groove 422; a wedge block 423 is installed on the bottom wall of the vertical groove 421, and the wedge block 423 includes an inclined surface 4231 corresponding to the bottom end of the inclined groove 422 and a table 4232 corresponding to the top end of the inclined groove 422. A groove 413 is provided on the inner wall of the button 41, and a second spring 414 is fixedly connected to the bottom wall of the groove 413. A pin 415 is provided at one end of the second spring 414 away from the bottom wall of the groove 413, and the pin 415 is adapted to the vertical groove 421 and the oblique groove 422. When the button 41 is pressed downward, the pin 415 will be driven to move along the oblique groove 422, from the top end of the oblique groove 422 to the bottom end of the oblique groove 422. During the movement of the pin 415 along the oblique groove 422, the knob 42 will be driven to rotate, thereby driving the containing container 5 to rotate, so that the arc-shaped incubation placement groove 51 mentioned above will be rotated to the notch 6 in sequence. As the pin 415 moves along the inclined groove 422, the first spring 412 will be compressed; therefore, after releasing the button 41, under the restoring action of the first spring 412, the pin 415 will move along the vertical groove 421 to its top end, and as the pin 415 passes over the wedge block 423, the inclined surface 4231 of the wedge block 423 squeezes the pin 415 into the groove 413, so that the pin 415 can pass over the wedge block 423 and reach the top end of the vertical groove 421. At this time, the table 4232 of the wedge block 423 will hinder the pin 415 from moving downward along the vertical groove 421.

[0026] It should be noted that the height of the inclined groove 422 is gradually raised from the top to the bottom, so that a first step 424 is formed between the bottom of the vertical groove 421 and the inclined groove 422; at the same time, the table 4232 forms a second step 425 between the top of the vertical groove 421 and the inclined groove 422. It can be understood that by providing the first step 424 and the second step 425, it is possible to ensure that the pin 415 moves along a set route, so that the rotation direction of the container 5 remains fixed and does not rotate in the opposite direction.

[0027] See also Figure 4The outer surface of the button 41 is provided with an annular protrusion 411, and the guide cylinder 3 is provided with a guide groove 31 adapted to the annular protrusion 411. The outer surface of the button 41 is wound with the first spring 412, and the two ends of the first spring 412 are fixedly connected to the annular protrusion 411 and the bottom wall of the guide groove 31.

[0028] See also Figure 1 , the bottom end of the containing container 5 is rotatably connected with a lifting plate 13, and a third spring 14 is arranged between the lifting plate 13 and the bottom wall of the box body 1, and the third spring 14 is a compression spring. A rubber pad 9 is arranged at the top of the containing container 5, and a handle 12 is arranged at one end of the lifting plate 13, and the handle 12 is slidably matched with the limiting groove on the side wall of the box body 1. It can be understood that when the handle 12 moves to the bottom end of the limiting groove, it will drive the rubber pad 9 of the containing container 5 to separate from the box cover 2, so as not to hinder the rotation of the containing container 5; when the handle 12 moves to the top end of the limiting groove, it will drive the rubber pad 9 of the containing container 5 to fit tightly with the box cover 2, which plays a good sealing role, so that when the antibody incubation box is violently shaken or turned upside down, the antibody solution 51 in the arc-shaped incubation placement groove is not easy to spill out and mix.

[0029] Preferably, a ball bearing is provided between the bottom wall of the containing container 5 and the lifting plate 13, and the ball bearing serves to support the containing container 5, so that the rotation of the containing container 5 is more stable, and at the same time, the force of the third spring 14 acting on the containing container 5 is more uniform, which is beneficial to improving the sealing performance of various parts of the containing container 5.

[0030] Please continue reading Figure 1 The box body 1 and the container 5 are threadedly connected with a liquid guide tube 10 through the threaded hole at the beginning, and a plug 11 is inserted at the end of the liquid guide tube 10. The liquid guide tube 10 is connected with the arc-shaped incubation placement groove 51. The liquid guide tube 10 is threadedly connected to the threaded hole opened on the box body 1 or the container 5, so as to facilitate the installation and removal of the liquid guide tube 10. By providing the liquid guide tube 10, it is convenient to add liquid, recycle or abandon the antibody solution, and effectively avoid the outflow of PVDF membrane or NC membrane during the liquid addition process; in addition, adding liquid through the liquid guide tube 10 can prevent the impact force on the PVDF membrane or NC membrane from being too large during the liquid addition, which affects the protein on the membrane.

[0031] Working principle of the utility model: When in use, first hold the handle 12 to drive the lifting plate 13 and the container 5 to move downward, so that the rubber pad 9 on the container 5 is no longer in contact with the box cover 2; at this time, press the button 41 with your finger to move the pin 415 from the top of the inclined groove 422 to the bottom of the inclined groove 422, and drive the knob 42 to rotate in the process, thereby driving the container 5 to rotate, so that each arc-shaped incubation placement groove 51 and the cold storage material placement groove 52 are rotated to the notch 6 in turn, and after opening the switch box cover 7, it is convenient to replace the PVDF membrane or NC membrane in the corresponding arc-shaped incubation placement groove 51. When the pin 415 moves to the bottom of the inclined groove 422, under the action of the first spring 412, the pin 415 passes over the wedge block 423 in the vertical groove 411 and returns to the top of the inclined groove 422, so as to facilitate the next rotation of the container 5. After the PVDF membrane or NC membrane is replaced, the handle 12 is released, and under the action of the third spring 14, the containing container 5 moves upward, so that the rubber pad 9 fits tightly with the box cover 2, thereby preventing the antibody solutions in adjacent grooves from mixing after the antibody incubation box is overturned; in addition, when the lifting plate 13 and the containing container 5 are driven downward by the handle 12, the liquid guide tube 10 is removed from the containing container 5 in advance; when the handle 12 is released and the containing container 5 is reset, the liquid guide tube 10 is installed on the containing container 5, so as to facilitate the addition of antibody solution to the arc-shaped incubation placement groove 51 through the liquid guide tube 10, thereby avoiding the impact on the PVDF membrane or NC membrane when liquid is directly added through the upper end opening of the arc-shaped incubation placement groove 51, thereby affecting the protein on the membrane.

[0032] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the utility model.

[0033] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "illustrative embodiments", "examples", "specific examples", or "some examples" means that the specific features, structures, materials, or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the utility model. In this specification, the schematic expressions of the above terms do not necessarily refer to the same embodiment or example.

[0034] Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. Mentioning "embodiment" in this article means that the specific features, structures or characteristics described in conjunction with the embodiment may be included in at least one embodiment of the present embodiment application. The appearance of this phrase in various positions in the specification is not necessarily the same embodiment, nor is it an independent or alternative embodiment that is mutually exclusive with other embodiments. It can be explicitly and implicitly understood by those skilled in the art that the embodiments described herein can be combined with other embodiments. Based on the embodiments in this application, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of this application.

[0035] Although the embodiments of the present invention have been shown and described, those skilled in the art will appreciate that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the claims and their equivalents.

Claims

1. An antibody incubation kit for Western blotting, characterized in that: The invention comprises a box body (1), wherein a storage container (5) is fitted in a gap inside the box body (1), and a plurality of arc-shaped incubation placement grooves (51) and cold storage material placement grooves (52) are arranged at intervals on the storage container (5); a box cover (2) is arranged at the upper end of the box body (1), and a switch box cover plate (7) is rotatably connected to the box cover (2) via a notch (6); a guide cylinder (3) is arranged in the middle of the box cover (2), and a push-rotate structure (4) is arranged inside the guide cylinder (3); when the push-rotate structure (4) is pressed, the push-rotate structure (4) will sequentially drive each arc-shaped incubation placement groove (51) and cold storage material placement groove (52) to move to the notch (6).

2. The antibody incubation kit for Western blotting according to claim 1, characterized in that: The push-rotation structure (4) comprises a button (41) and a knob (42) that are slidably matched with each other, and the button (41) is slidably arranged in the guide tube (3), and the knob (42) is installed in the middle of the bottom wall of the containing container (5), and a first spring (412) is arranged between the button (41) and the guide tube (3). A plurality of vertical grooves (421) are distributed in a circular array on the outer wall of the knob (42), and the top and bottom ends of adjacent vertical grooves (421) are connected to each other through an inclined groove (422); A wedge block (423) is installed, the wedge block (423) comprising an inclined surface (4231) corresponding to the bottom end of the inclined groove (422) and a table surface (4232) corresponding to the top end of the inclined groove (422); a groove (413) is provided on the inner wall of the button (41); a second spring (414) is fixedly connected to the bottom wall of the groove (413); a pin shaft (415) is provided at one end of the second spring (414) away from the bottom wall of the groove (413); the pin shaft (415) is adapted to the vertical groove (421) and the inclined groove (422).

3. The antibody incubation kit for Western blotting according to claim 2, characterized in that: The button (41) is provided with an annular protrusion (411) on its outer surface, the guide cylinder (3) is provided with a guide groove (31) adapted to the annular protrusion (411), the first spring (412) is wound around the outer surface of the button (41), and two ends of the first spring (412) are fixedly connected to the annular protrusion (411) and the bottom wall of the guide groove (31).

4. The antibody incubation kit for Western blotting according to claim 2, characterized in that: The height of the inclined groove (422) gradually increases from the top to the bottom, so that a first step (424) is formed between the bottom of the vertical groove (421) and the inclined groove (422), and a second step (425) is formed between the top of the vertical groove (421) and the inclined groove (422).

5. An antibody incubation kit for Western blotting according to any one of claims 1 to 4, characterized in that: The bottom end of the containing container (5) is rotatably connected to a lifting plate (13), a third spring (14) is arranged between the lifting plate (13) and the bottom wall of the box body (1), a rubber pad (9) is arranged at the top end of the containing container (5), and a handle (12) is arranged at one end of the lifting plate (13), and the handle (12) is slidably matched with a limiting groove on the side wall of the box body (1).

6. The antibody incubation kit for Western blotting according to claim 5, characterized in that: A ball bearing is provided between the bottom wall of the containing container (5) and the lifting plate (13).

7. The antibody incubation kit for Western blotting according to claim 1, characterized in that: A liquid guide tube (10) is threadedly connected to the box body (1) and the containing container (5) via a threaded hole, a plug (11) is inserted at the end of the liquid guide tube (10), and the liquid guide tube (10) is connected to the arc-shaped incubation placement groove (51).

8. The antibody incubation kit for Western blotting according to claim 1, characterized in that: The number of the arc-shaped incubation placement grooves (51) and the cold storage material placement grooves (52) is set to eight.

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