Automatic split charging device

Abstract

The utility model discloses an automatic subpackaging device. The device comprises a cell suspension refrigerating and shaking device and a closed pipeline control device, the cell suspension refrigerating and shaking device comprises a base, a box body, a semiconductor refrigerating structure, a power source, a transmission mechanism, a temperature sensor, a temperature controller and a controller, the temperature sensor is arranged in the box body, and the temperature sensor is connected with the temperature controller; the temperature controller is connected with the semiconductor refrigeration structure; the transmission mechanism is connected with the semiconductor refrigeration structure; a cell suspension bag is placed in the box body; the closed pipeline control device comprises a pipeline, a bracket, a control unit, a peristaltic pump, an air filter, a bubble sensor group and a pinch valve group, one end of the pipeline is connected with the cell suspension bag, the other end of the pipeline passes through the peristaltic pump, and the pipeline is also connected with a plurality of branch pipes and the air filter; the base is connected to the support. The utility model can solve the problems that the existing device has high requirements on the operating environment, low split charging precision, poor uniform mixing effect, inaccurate temperature control and the like.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture, in particular to an automatic packaging device. Background Art

[0002] In the process of cell culture, packaging is a crucial link. The existing packaging methods mainly include open packaging. This type of device has high requirements for the background environment and needs to be operated under sterile conditions to prevent contamination risks. There is insufficient calibration, resulting in low packaging accuracy and large deviation in repeatability. Incomplete bubble removal may lead to instability in the cell culture process. During freezing, liquid bags are prone to bulging and there is a certain risk. In the shaking process, most of them use vertical hanging liquid bags and mix them by horizontal squeezing. Since the center of gravity does not change, the mixing effect of cell sedimentation is not good, and there is a possibility of uneven distribution of cells. Moreover, most packaging equipment uses open space open-loop refrigeration control, and the temperature control is not accurate enough, and condensation may occur, affecting the stability of cell culture.

[0003] Therefore, it is necessary to design a new device to solve the problems of the existing device having high requirements for the operating environment, low packaging accuracy, poor mixing effect and inaccurate temperature control. Utility Model Content

[0004] The utility model aims to overcome the defects of the prior art and provide an automatic dispensing device.

[0005] In order to solve the above technical problems, the purpose of the utility model is achieved through the following technical solutions: to provide an automatic packaging device, including: a cell suspension refrigeration and shaking device and a closed pipeline control device; wherein, the cell suspension refrigeration and shaking device includes a base, a box body, a semiconductor refrigeration structure, a power source, a transmission mechanism, a temperature sensor, a temperature controller and a controller, the temperature sensor is built into the box body, the temperature sensor is connected to the temperature controller; the temperature controller is connected to the semiconductor refrigeration structure; the transmission mechanism is connected to the semiconductor refrigeration structure; and the transmission mechanism is connected to the base; the box body is connected to the semiconductor refrigeration structure, the controller is connected to the power source; the power source is connected to the transmission mechanism, and a cell suspension placed parallel to the bottom of the box body is placed in the box body. bag; the closed pipeline control device includes a pipeline, a bracket, a control unit, a peristaltic pump, an air filter, a bubble sensor group and a clamp valve group, the bubble sensor group and the clamp valve group are respectively connected to the bracket, the peristaltic pump is connected to the bracket, one end of the pipeline is connected to the cell suspension bag, the other end of the pipeline passes through the peristaltic pump, the other end of the pipeline is also connected to a plurality of branch pipes, the branch pipes are connected to the sub-packaging bag, the bubble sensor group is provided with a first slot body, and the branch pipe is clamped in the first slot body; the clamp valve group is provided with a second slot body, and the branch pipe is clamped in the second slot body; the pipeline is also connected to the air filter, the control unit is respectively connected to the peristaltic pump, the bubble sensor group and the clamp valve group; the base is connected to the bracket.

[0006] Its further technical solution is: a cavity for placing a cell suspension bag is provided in the box body; the lower end surface of the box body is connected to the semiconductor refrigeration structure; and the side wall and upper end surface of the cavity are respectively connected with thermal insulation foam.

[0007] Its further technical solution is: the semiconductor refrigeration structure includes a shell with an opening at the upper end, a semiconductor refrigeration plate, a heat pipe, a heat sink and a fan; the semiconductor refrigeration plate, the heat pipe, the heat sink and the fan are respectively installed in the shell, and the opening of the shell is connected to the lower end surface of the box body.

[0008] Its further technical solution is: the transmission mechanism includes a first synchronous wheel, a synchronous belt, a rotating shaft and a second synchronous wheel, the power source includes a first output shaft, the first output shaft is connected to the first synchronous wheel; the second synchronous wheel is connected to the rotating shaft; the other end of the rotating shaft passes through the housing and is connected to the housing; the second synchronous wheel is connected to the first synchronous wheel through the synchronous belt.

[0009] A further technical solution is as follows: the bubble sensor group includes a first bubble sensor, the pinch valve group includes a first pinch valve structure, and the other end of the pipeline passes through the first bubble sensor and the first pinch valve structure in sequence.

[0010] Its further technical solution is: the bubble sensor group includes a plurality of second bubble sensors, the pinch valve group also includes a plurality of second pinch valve structures, one end of the branch pipe is connected to the pipeline, and the other end of the branch pipe is connected to the packaging bag after passing through the second pinch valve structure and the second bubble sensor in sequence.

[0011] Its further technical solution is: the bubble sensor group also includes a third bubble sensor and a fourth bubble sensor, and the other end of the pipeline passes through the third bubble sensor and the fourth bubble sensor in sequence; the pinch valve group also includes a third pinch valve structure and a fourth pinch valve structure, and the other end of the pipeline passes through the third bubble sensor, the fourth bubble sensor, the third pinch valve structure, and the fourth pinch valve structure in sequence and then returns to one end of the pipeline connected to the peristaltic pump.

[0012] A further technical solution is: the air filter is connected to the pipeline between the fourth bubble sensor and the third pinch valve structure.

[0013] Its further technical solution is: a liquid bag outlet pipe fixing block is installed on the bracket, the other end of the branch pipe is connected to the sub-packaging bag outlet pipe, and the sub-packaging bag outlet pipe is connected to the liquid bag outlet pipe fixing block.

[0014] A further technical solution is that the dispensing bag outlet pipe is connected to the dispensing bag.

[0015] Compared with the prior art, the utility model has the following beneficial effects: the utility model uses a cell suspension refrigeration and shaking device and a closed pipeline control device, and utilizes the cell suspension refrigeration and shaking device to perform horizontal large-angle reciprocating oscillation of the cell suspension bag and reciprocating oscillation of the center of gravity back and forth, so as to effectively avoid the cells from sinking to the bottom and improve the mixing effect; the cell suspension bag is placed in a closed low-temperature box, and a closed-loop control method is adopted to ensure accurate temperature control and avoid the generation of condensed water, and the cell activity can be effectively maintained during the large-volume and long-term packaging process; the closed pipeline control device is a closed pipeline packaging method, which has low requirements for the background environment and reduces the risk of pollution; it solves the problems of the existing device having high requirements for the operating environment, low packaging accuracy, poor mixing effect and inaccurate temperature control.

[0016] The utility model is further described below in conjunction with the accompanying drawings and specific embodiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the technical solutions of the embodiments of the utility model, the drawings required for use in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0018] Figure 1 A schematic diagram of the three-dimensional structure of an automatic packaging device provided in an embodiment of the utility model;

[0019] Figure 2 A schematic diagram of the three-dimensional structure of a cell suspension refrigeration and shaking device provided in an embodiment of the utility model;

[0020] Figure 3 A schematic diagram of the side structure of a cell suspension cooling and shaking device provided in an embodiment of the utility model;

[0021] Figure 4 A schematic diagram of the explosion structure of the cell suspension refrigeration and shaking device provided in an embodiment of the utility model;

[0022] Figure 5 A schematic diagram of the exploded structure of the transmission mechanism provided in the embodiment of the utility model;

[0023] Figure 6 A schematic diagram of the three-dimensional structure of a closed pipeline control device provided by an embodiment of the utility model;

[0024] Figure 7 A schematic diagram of the top view of the closed pipeline control device provided by an embodiment of the utility model;

[0025] Figure 8 A schematic diagram of the front view of a first pinch valve structure provided by an embodiment of the utility model;

[0026] Fig. 9 A schematic diagram of an exploded structure of a first pinch valve structure provided by an embodiment of the utility model;

[0027] Fig.10 A schematic diagram of a clamping state of a first pinch valve structure provided in an embodiment of the utility model (without the protective cover);

[0028] Fig.11 A schematic diagram of a first pinch valve structure in a loosened state (without the protective cover) provided by an embodiment of the utility model;

[0029] Description of the symbols in the figure:

[0030] 1. Cell suspension refrigeration and shaking device 1; 2. Closed pipeline control device; 10. Base; 20. Box body; 30. Semiconductor refrigeration structure; 40. Power source; 50. Transmission mechanism; 51. First synchronous wheel; 52. Synchronous belt; 53. Rotating shaft; 54. Second synchronous wheel; 60. Temperature controller; 70. Controller; 80. Power input port; 90. Pipeline; 100. Bracket; 111. Liquid bag outlet fixing block; 120. Peristaltic pump; 130. Air filter; 140. First bubble sensor; 141. Second bubble sensor; 142. 2. The third bubble sensor; 143. The fourth bubble sensor; 150. The first pinch valve structure; 1510. The cylinder; 1511. The output shaft; 1520. The push rod sleeve; 1521. The connecting rod; 1522. The limit rod; 1530. The connecting block; 1531. The connecting rod; 1532. The pinch valve block; 1540. The protective cover; 1550. The fixing block; 151. The second pinch valve structure; 152. The third pinch valve structure; 153. The fourth pinch valve structure; 160. The branch; 170. The status indicator light; 180. The touch screen. DETAILED DESCRIPTION

[0031] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0032] It should be understood that when used in this specification and the appended claims, the terms "include" and "comprises" indicate the presence of described features, integers, steps, operations, elements and / or components, but do not exclude the presence or addition of one or more other features, integers, steps, operations, elements, components and / or combinations thereof.

[0033] It should also be understood that the terms used in this utility model specification are only for the purpose of describing specific embodiments and are not intended to limit the utility model. As used in this utility model specification and the appended claims, unless the context clearly indicates otherwise, the singular forms "a", "an" and "the" are intended to include plural forms.

[0034] It should be further understood that the term “and / or” used in the present specification and the appended claims refers to any combination and all possible combinations of one or more of the associated listed items, and includes these combinations.

[0035] See also Figure 1 , Figure 1A three-dimensional structural schematic diagram of an automatic packaging device provided in an embodiment of the utility model can be used in the process of refrigeration, shaking and packaging of cell suspensions to solve the problems of existing devices such as high requirements on the operating environment, low packaging accuracy, poor mixing effect and inaccurate temperature control.

[0036] See also Figure 1 The above-mentioned automatic packaging device includes: a cell suspension refrigeration and shaking device 1 and a closed pipeline control device 2; wherein, the cell suspension refrigeration and shaking device 1 includes: a base 10, a box body 20, a semiconductor refrigeration structure 30, a power source 40, a transmission mechanism 50, a temperature sensor, a temperature controller 60 and a controller 70, the temperature sensor is built in the box body 20, and the temperature sensor is connected to the temperature controller 60; the temperature controller 60 is connected to the semiconductor refrigeration structure 30; the transmission mechanism 50 is connected to the semiconductor refrigeration structure 30; and the transmission mechanism 50 is connected to the base 10; the box body 20 is connected to the semiconductor refrigeration structure 30, the controller 70 is connected to the power source 40; the power source 40 is connected to the transmission mechanism 50, and a cell suspension bag is placed in the box body 20 parallel to the bottom of the box body 20.

[0037] The closed pipeline control device 2 includes a pipeline 90, a bracket 100, a control unit, a peristaltic pump 120, an air filter 130, a bubble sensor group and a clamp valve group. The bubble sensor group and the clamp valve group are respectively connected to the bracket 100, the peristaltic pump 120 is connected to the bracket 100, one end of the pipeline 90 is connected to the cell suspension bag, the other end of the pipeline 90 passes through the peristaltic pump 120, the other end of the pipeline 90 is also connected to a plurality of branch pipes 160, the branch pipes 160 are connected to the sub-packaging bag, the bubble sensor group is provided with a first slot body, and the branch pipe 160 is stuck in the first slot body; the clamp valve assembly is provided with a second slot body, and the branch pipe 160 is stuck in the second slot body. The pipeline 90 is also connected to the air filter 130, and the control unit is respectively connected to the peristaltic pump 120, the air filter 130, the bubble sensor group and the clamp valve group.

[0038] The base 10 is connected to the bracket 100;

[0039] Specifically, first, all the pipes and branch pipes 160, etc., as well as the cell suspension bag and the sub-packaging bag are installed, and the pipes are connected. Then, when the device is powered on, the temperature sensor will monitor the temperature inside the box body 20. If the temperature is higher than the preset temperature, the temperature sensor will trigger the relay to output a signal to the thermostat 60, and the thermostat 60 will drive the power supply to transmit power to the semiconductor refrigeration structure 30. After the semiconductor refrigeration structure 30 is powered on, it uses the movement of electrons to transfer heat from one side to the other side; then, the heat is dissipated through the heat pipe and the cooling fan to achieve a cooling effect. If the temperature is equal to the preset temperature, the temperature sensor will disconnect the relay output signal, and under the drive of the thermostat 60, the power supply will disconnect the power supply to the semiconductor refrigeration structure 30, causing the semiconductor refrigeration structure 30 to stop working to maintain the device at the target temperature level.

[0040] See also Figure 3 , by default, the box body 20 will turn to the right limit position. The preset mixing program can be run by pressing the button on the controller 70. The controller 70 will continue to send a left turn command to the driver, and the driver will continue to send a pulse signal to the power source 40 to make the power source 40 turn left until the left limit sensor detects a signal. Then, the controller 70 will continue to send a right turn command to the driver, and the driver will continue to send a pulse signal to the power source 40 to make the power source 40 turn right until the right limit sensor detects a signal. Such a reciprocating cycle allows the mixing movement of the cell suspension in the cell suspension bag in the box body 20 to continue.

[0041] The packing bag is horizontally reciprocated at a large inclination angle (±30°) and the center of gravity is reciprocated back and forth, which can effectively avoid sinking to the bottom and ensure the density consistency of the packing; in addition, the cell suspension bag is placed in a closed box body 20 for closed-loop control, and the temperature control is accurate, which can well maintain cell activity during large-volume and long-term packing.

[0042] Secondly, the liquid in the cell suspension bag is transported to the peristaltic pump 120 through the pipeline 90 after being shaken, and the peristaltic pump 120 is responsible for pushing the liquid to the other end of the pipeline 90. The other end of the pipeline 90 is connected with several branch pipes 160, and the bubble sensor group is connected with the branch pipe 160 to detect whether there are bubbles in the pipeline 90 and remove them in time. The clamp valve group is connected with the branch pipe 160 to control the flow direction and flow rate of the liquid and realize the subpackaging function. The pipeline 90 is connected with an air filter 130 for filtering impurities in the air to ensure the purity of the liquid during the transmission process. The control unit accurately controls the peristaltic pump 120, the air filter 130, the bubble sensor group and the clamp valve group to ensure that the calibration, subpackaging and exhaust processes are stable and reliable.

[0043] In one embodiment, see Figure 4The box body 20 is provided with a cavity for placing a cell suspension bag; the lower end surface of the box body 20 is connected to the semiconductor refrigeration structure 30.

[0044] In one embodiment, the side walls and the upper end surface of the cavity are respectively connected with thermal insulation foam.

[0045] In this embodiment, the top surface of the box body 20 is a door panel, which is provided with a handle for opening the door panel. Insulating foam is attached between the other surfaces and the top surface to reduce the heat exchange between the box body 20 and the outside world, thereby preventing the generation of condensed water and affecting cell activity.

[0046] In one embodiment, the semiconductor refrigeration structure 30 includes a shell with an opening at the upper end, a semiconductor refrigeration plate, a heat pipe, a heat sink and a fan; the semiconductor refrigeration plate, the heat pipe, the heat sink and the fan are respectively installed in the shell, and the opening of the shell is connected to the lower end surface of the box body 20.

[0047] The housing is fixed on the base 10 to ensure the stability and sealing of the housing. The housing is usually the outer shell of the device, which is used to protect the internal components and provide external support.

[0048] The semiconductor cooling chip is an important component for providing cooling effect. Generally, the semiconductor cooling chip should be installed at a suitable position inside the shell to ensure that it can effectively reduce the temperature. The specific installation position is close to the opening, close to the lower end surface of the box body 20.

[0049] The function of the heat pipe is to transfer heat from the heat source to the heat sink and fan for heat dissipation. The heat pipe is installed between the semiconductor cooling fin and the heat sink to ensure that the heat can be effectively transferred to the cooling system.

[0050] The heat sink is installed at the end of the heat pipe to diffuse and dissipate the heat. The heat sink is usually designed with a large area and good heat dissipation performance to ensure that the heat is effectively transferred to the surrounding environment.

[0051] The fan is installed near the heat sink to accelerate air flow and enhance heat dissipation. The fan can be directly fixed on the heat sink to provide better air circulation.

[0052] In one embodiment, see Figure 5 The transmission mechanism 50 includes a first synchronous wheel 51, a synchronous belt 52, a rotating shaft 53 and a second synchronous wheel 54. The power source 40 includes a first output shaft, which is connected to the first synchronous wheel 51; the second synchronous wheel 54 is connected to the rotating shaft 53; the other end of the rotating shaft 53 passes through the shell and is connected to the shell; the second synchronous wheel 54 is connected to the first synchronous wheel 51 through the synchronous belt 52.

[0053] The transmission mechanism 50 further includes a housing, in which the first synchronous wheel 51 , the synchronous belt 52 and the second synchronous wheel 54 are built.

[0054] When the power source 40 (first output shaft) provides power, the first synchronous wheel 51 is driven to rotate by the rotation of the output shaft. The synchronous belt 52 transmits the movement of the first synchronous wheel 51 to the second synchronous wheel 54, thereby driving the rotating shaft 53 to rotate. The other end of the rotating shaft 53 passes through the shell and is connected to the shell, so when the rotating shaft 53 rotates, the shell will also rotate, and when the shell rotates, the box body 20 will also rotate, so that the cell suspension in the box body 20 is shaken evenly.

[0055] Specifically, the other end of the rotating shaft 53 is connected to the housing through a bearing, ensuring that the rotating shaft 53 can rotate freely inside the housing while maintaining the structural stability of the housing. The bearing can reduce the friction between the rotating shaft 53 and the housing and support the rotation of the rotating shaft 53, thereby achieving smooth movement. Overall, such a design enables the output of the power source 40 to drive the housing to rotate through the transmission mechanism 50 to complete the corresponding functional operation.

[0056] In one embodiment, a left limit sensor and a right limit sensor are installed on the power source 40 , and the left limit sensor and the right limit sensor are connected to the controller 70 , respectively.

[0057] In this embodiment, the left limit sensor and the right limit sensor are mainly limit switches.

[0058] The left limit sensor and the right limit sensor are installed on the power source 40, and their function is to detect the rotation position of the power source 40. Specifically, when the power source 40 (which may be the first output shaft) rotates to a specific position, the left limit sensor and the right limit sensor will detect this position and send a signal to the controller 70. For example, when the power source 40 rotates to the left limit position, the left limit sensor will detect this position and send a signal to the controller 70 to instruct the controller 70 to stop or change the movement direction of the power source 40. Similarly, when the power source 40 rotates to the right limit position, the right limit sensor will send a signal to the controller 70 to instruct the controller 70 to stop or change the movement direction of the power source 40.

[0059] In this way, through the position detection of the left limit sensor and the right limit sensor, the controller 70 can ensure that the power source 40 moves within a specific range and avoids exceeding the set limit position.

[0060] In one embodiment, the controller 70 is connected to the power source 40 via a driver.

[0061] In one embodiment, see Figure 2 The base 10 is provided with a power input port 80 .

[0062] In one embodiment, see Figure 2 The power input port 80 is connected to the semiconductor refrigeration structure 30 and the power source 40 respectively.

[0063] In this embodiment, the power source 40 includes a stepping motor.

[0064] The above-mentioned cell suspension refrigeration and shaking device 1 is provided with a base 10, a box body 20, a semiconductor refrigeration structure 30, a power source 40, a transmission mechanism 50, a temperature sensor, a temperature controller 60 and a controller 70. The temperature in the box body 20 is detected by the temperature sensor, and the temperature is controlled by the semiconductor refrigeration structure 30. In addition, the power source 40 and the transmission mechanism 50 are used to drive the box body 20 to rotate, so that the cell suspension bag is horizontally reciprocated at a large angle; the cells or sediments in the liquid are effectively prevented from being deposited at the bottom, the liquid is mixed more evenly, the mixing efficiency is improved, and the temperature can be ensured to be stable within a set range, while reducing the generation of condensed water.

[0065] In one embodiment, see Figure 6 and Figure 7 The bubble sensor group includes a first bubble sensor 140, the pinch valve group includes a first pinch valve structure 150, and the other end of the pipeline 90 passes through the first bubble sensor 140 and the first pinch valve structure 150 in sequence.

[0066] In one embodiment, see Figure 6 and Figure 7 The bubble sensor group includes a plurality of second bubble sensors 141, and the clamping valve group also includes a plurality of second clamping valve structures 151. One end of the branch pipe 160 is connected to the pipeline 90, and the other end of the branch pipe 160 is connected to the packaging bag after passing through the second clamping valve structure 151 and the second bubble sensor 141 in sequence.

[0067] In one embodiment, see Figure 6 and Figure 7 The bubble sensor group further includes a third bubble sensor 142 and a fourth bubble sensor 143 , and the other end of the pipe 90 passes through the third bubble sensor 142 and the fourth bubble sensor 143 in sequence.

[0068] The first bubble sensor 140 , the second bubble sensor 141 , the third bubble sensor 142 , and the fourth bubble sensor 143 are all provided with a first groove.

[0069] In one embodiment, see Figure 6 and Figure 7The clamping valve group also includes a third clamping valve structure 152 and a fourth clamping valve structure 153. The other end of the pipeline 90 passes through the third bubble sensor 142, the fourth bubble sensor 143, the third clamping valve structure 152, and the fourth clamping valve structure 153 in sequence and then returns to one end of the pipeline 90 connected to the peristaltic pump 120.

[0070] In one embodiment, see Figure 6 and Figure 7 The air filter is connected to the pipeline 90 located between the fourth bubble sensor 143 and the third pinch valve structure 152.

[0071] In one embodiment, see Figure 6 and Figure 7 , a plurality of branch pipes 160 are arranged at intervals on both sides of the pipeline 90 .

[0072] In one embodiment, see Figure 6 and Figure 7 A liquid bag outlet pipe fixing block 111 is installed on the bracket 100 , and the other end of the branch pipe 160 is connected to the sub-packaging bag outlet pipe, and the sub-packaging bag outlet pipe is connected to the liquid bag outlet pipe fixing block 111 .

[0073] In one embodiment, see Figure 6 and Figure 7 , the dispensing bag outlet pipe is connected to the dispensing bag.

[0074] The bracket 100 is provided with an inclined surface which is inclined outward from top to bottom, and the above-mentioned liquid bag outlet pipe fixing block 111 is installed on the inclined surface. The setting of the inclined surface can make it easier to discharge the bubbles in the scoring bag due to buoyancy.

[0075] During use, since disposable consumables need to be reinstalled each time, such as the peristaltic pump 120, and the peristaltic pump 120 has certain differences each time it is installed, the device of this embodiment can be used for calibration. Specifically, after all consumables are installed, each branch pipe 160 and the pipeline 90 can be stuck on the corresponding components, such as the pinch valve structure and the bubble sensor and other components, and the pipeline 90 between the third bubble sensor 142 and the fourth bubble sensor 143 is determined to be Vml, the first pinch valve structure 150 and the third pinch valve structure 152 are opened, and the branch pipes 160 and the pipelines 90 are closed. With the second pinch valve structure 151, the peristaltic pump 120 rotates clockwise. When the first bubble sensor 140 detects liquid, that is, when the signal switches from 0 to 1, the motor of the peristaltic pump 120 is reset and starts counting. When the third bubble sensor 142 detects liquid, the number of revolutions of the motor of the current peristaltic pump 120 is recorded as N1. When the fourth bubble sensor 143 detects liquid, the number of revolutions of the motor of the current peristaltic pump 120 is recorded as N2. The precise pump speed of the peristaltic pump 120 after the installation of the consumables is calculated to be V / (N2-N1), in units of ml / revolution.

[0076] Furthermore, the subpackaging and exhausting process of the device of this embodiment is as follows:

[0077] After the consumables are correctly installed, each branch tube 160 and pipeline 90 can be stuck on the corresponding components, such as the pinch valve structure and the bubble sensor, and the pump speed calibration process is carried out. At the same time, the pipeline 90 is filled, and the third pinch valve structure 152 is closed to open all the second pinch valve structures 151, and the cell suspension is pumped into the sub-packaging bag connected to the corresponding branch tube 160 according to the set and actual pump speed, and then all the second pinch valve structures 151 are closed, and the third pinch valve structure 152 is opened. The peristaltic pump 120 rotates counterclockwise to inhale air through the air filter 130 and push the cell suspension recovered to the pipeline 90 until the first bubble sensor 140 detects the air; the third pinch valve structure 152 and the first pinch valve structure 150 are closed; the fourth pinch valve structure 151 is opened. 3. The peristaltic pump 120 rotates clockwise to open all the second pinch valve structures 151 in sequence, and after the cell suspension in each corresponding branch tube 160 is injected into the sub-packaging bag through air according to the volume of the branch tube 160, all the second pinch valve structures 151 are closed; the process of opening all the second pinch valve structures 151 is to open the first second pinch valve structure 151, and the peristaltic pump 120 rotates counterclockwise to discharge the air in the corresponding branch tube 160 and the liquid belt through the air filter 130 until the first second bubble sensor 141 detects the liquid. At this time, the gas is completely discharged and the cell suspension is not lost. The sub-packaging bag is tilted and fixed to keep the bubbles at the outlet position. According to this process, the branch tubes 160 corresponding to other second pinch valve structures 151 are vented in sequence.

[0078] The above-mentioned device realizes the functions of calibration, subpackaging and exhaust, and improves the automation and stability of the production process. Through the cooperation of the bubble sensor group and the clamping valve group, the bubbles in the pipeline 90 can be detected and eliminated in time to ensure the stability and quality of liquid transmission. The application of the air filter 130 can effectively filter impurities in the air and improve the purity of the liquid. The control unit accurately controls each component to ensure the stability and reliability of the entire liquid transmission process. The application of multiple sensors and valve structures makes the device more flexible and diverse, suitable for different production needs.

[0079] In one embodiment, the above-mentioned first clamping valve structure 150, second clamping valve structure 151 and third clamping valve structure 152 are the same, and all include: a cylinder 1510, a coupling assembly, a clamping assembly and a protective cover 1540, the cylinder 1510 is connected to the coupling assembly; the coupling assembly is connected to the clamping assembly, the clamping assembly is built into the protective cover 1540, the clamping assembly includes a connecting rod structure and a clamping block 1532, the clamping block 1532 is connected to the connecting rod structure; the connecting rod structure is connected to the coupling assembly, the clamping block 1532 is rotatably connected to the protective cover 1540, and a second trough body is provided in the clamping assembly.

[0080] In this embodiment, when the cylinder 1510 outputs thrust, the thrust is first transmitted to the coupling assembly, and then transmitted to the clamping assembly through the coupling assembly. After the clamping assembly is acted upon by the thrust, it rotates under the rotation connection with the protective cover 1540, thereby realizing the function of clamping or releasing the liquid pipe by the clamping block 1532.

[0081] In one embodiment, see Figure 8 and Fig. 9 The above-mentioned cylinder 1510 includes an output shaft 1511; the coupling assembly includes a push rod sleeve 1520 and a coupling push rod 1521, one end of the push rod sleeve 1520 is connected to the output shaft 1511; one end of the coupling push rod 1521 is built into the push rod sleeve 1520, and one end of the coupling push rod 1521 is connected to the clamping tube assembly.

[0082] In one embodiment, see Figure 8 and Fig. 9 The above-mentioned coupling assembly also includes a limiting rod 1522, which is installed between the push rod sleeve 1520 and the coupling push rod 1521.

[0083] Specifically, the cylinder 1510 includes an output shaft 1511, which is connected to the coupling assembly, and the thrust output by the cylinder 1510 is transmitted to the coupling assembly. The coupling assembly includes a push rod sleeve 1520 and a coupling push rod 1521, one end of the push rod sleeve 1520 is connected to the output shaft 1511, and one end of the coupling push rod 1521 is built into the push rod sleeve 1520 and connected to the clamping tube assembly. The limit rod 1522 is installed between the push rod sleeve 1520 and the coupling push rod 1521. Among them, the push rod sleeve 1520 is sleeved on the outer ring of the coupling push rod 1521, and is concentrically matched, and the limit rod 1522 is provided in the center, which serves as the middle part connecting the clamping tube part and the cylinder 1510. One end of the push rod sleeve 1520 is connected to the output shaft 1511 of the cylinder 1510 through a threaded connection, and the other end of the coupling push rod 1521 is connected to the connecting block 1530 of the clamping tube part through a pin.

[0084] It can be seen that the coupling part has a certain stroke buffer part, which is used to convert the large stroke of the cylinder 1510 into the small stroke of the clamping part. The overall structural design enables the clamping valve to achieve a relatively large stroke conversion when the volume is relatively small, so that the movement of the clamping part can be controlled more flexibly and accurately in practical applications. The setting of the limit rod 1522 also helps to control the stroke range of the clamping part, thereby improving the control accuracy and reliability of the clamping valve.

[0085] The pinch valve has a small overall volume, but can transform the large stroke of the cylinder 1510 into a small stroke of the pinch part, thereby improving the compactness and efficiency of the system. The setting of the limit rod 1522 and the design of the coupling part enable the pinch valve to achieve precise stroke control, meeting the requirements for high movement accuracy of the pinch part. By designing a reasonable coupling part, the stability and reliability of the pinch valve are enhanced, the service life is extended, and the maintenance cost is reduced.

[0086] In summary, this pinch valve structure design can realize the conversion of large stroke into small stroke under the premise of small volume, improves the control accuracy and reliability, and is suitable for industrial application environments that require precise control.

[0087] In one embodiment, see Figures 9 to 11 The above-mentioned connecting rod structure includes a connecting block 1530 and a connecting rod 1531. The connecting block 1530 is connected to the connecting push rod 1521; one end of the tube clamping block 1532 is connected to the connecting push rod 1521 through the connecting block 1530, and the end of the tube clamping block 1532 connected to the connecting block 1530 is rotatably connected to the protective cover 1540.

[0088] In one embodiment, see Figures 9 to 11The number of the tube clamping blocks 1532 is two; the two tube clamping blocks 1532 are symmetrically arranged along the horizontal axis of the connecting block 1530. A second slot is formed between the two tube clamping blocks 1532.

[0089] In one embodiment, see Figures 9 to 11 The connecting block 1530 is connected to the connecting rod 1521 via a pin.

[0090] In one embodiment, see Figures 8 to 9 The protective cover 1540 is provided with a cavity for placing the pipe clamping block 1532 .

[0091] In one embodiment, see Figures 8 to 9 One end of the tube clamping block 1532 connected to the connecting block 1530 is connected to the protective cover 1540 through a pin shaft.

[0092] In this embodiment, the connecting rod 1531, the connecting block 1530 and the tube clamping block 1532 form a connecting rod structure, and the connecting rod 1531 is connected to the push rod flat core and the tube clamping block 1532 by a pin shaft connection. The tube clamping block 1532 is fixed in the protective cover 1540 and rotates around the pin shaft.

[0093] The clamp assembly adopts a connecting rod structure and is composed of small parts, which makes the overall volume small and suitable for places with limited space. The clamp valve can completely clamp the liquid pipe to ensure the blocking of liquid flow, has a large clamping force, and improves the stability and reliability of the clamp. The clamp assembly is designed with a large loose clamping angle, which will not affect the normal installation of the pipe 90, ensuring the flexibility of the arrangement and connection of the pipe 90.

[0094] In one embodiment, see Figures 8 to 11 , a pinch valve structure also includes a fixing block 1550 , one end of which is connected to the cylinder 1510 .

[0095] In one embodiment, see Figures 8 to 11 , the coupling assembly is built into the fixed block 1550.

[0096] In one embodiment, the protective cover 1540 is fixed to the other end of the fixing block 1550 and is fixed by screws to limit the clamping assembly and protect the connecting block 1530 of the clamping assembly from being damaged by collision, thereby extending the service life.

[0097] In this embodiment, the above-mentioned structure uses pneumatic elements to drive the internal mechanical structure to control the clamping block 1532 to clamp or loosen, so that the clamping block 1532 can clamp the liquid tube. The structure is fixed and stable, which is more stable than the electric drive. The reasonable structural layout saves more space. Specifically, the connecting rod structure is used to convert the linear motion of the cylinder 1510 into the rotational motion of the clamping part, thereby realizing the conversion of functions, thereby clamping the liquid tube and blocking the flow of liquid. Through the design of the buffered coupling assembly and the connecting rod structure, the transmission of the movement of the cylinder 1510 to the clamping assembly is realized in a smaller space, saving the installation space of the system. The use of the connecting rod structure can make the clamping force of the clamping assembly stable and reliable, ensuring the effect of clamping the tube.

[0098] By setting up a cylinder 1510, a coupling assembly, a tube clamping assembly and a protective cover 1540, wherein the cylinder 1510 drives the coupling assembly to perform linear motion and transmits it to the tube clamping assembly, and the connecting rod structure in the tube clamping assembly converts the linear telescopic motion into the loosening or closing of the tube clamping block 1532, thereby overcoming the problems of insufficient motor thrust and inadequate stroke, and improving the stability and reliability of the tube clamping valve.

[0099] The above-mentioned closed pipeline control device 2, by setting up a pipeline 90, a bracket 100, a control unit, a peristaltic pump 120, an air filter 130, a bubble sensor group and a clamping valve group, utilizes the ingenious coordination of the pipeline 90, the branch pipe 160, the peristaltic pump 120, the air filter 130, the bubble sensor group and the clamping valve group, and is controlled by the control unit to realize the use of a sealed pipeline 90 system, which has lower background environment requirements and pollution risks, higher packaging accuracy, more thorough bubble removal, and safer and more reliable during freezing.

[0100] In this embodiment, the control unit may be integrated into the controller 70 .

[0101] In one embodiment, the bracket 100 is provided with a touch screen 180 for controlling the entire device when used alone, and can also be computer controlled after being connected to the Internet. In addition, the bracket 100 is provided with a status indicator light 170 for indicating the current status of the device.

[0102] When in use, install the pipeline 90 and other related kits on the bracket 100 according to the logo and instructions, install the cell suspension bag into the box body 20, and connect the corresponding pipelines 90 of the two together; input parameters through the host computer or touch screen 180 to start the whole process of subpackaging; the refrigeration function continues to work, and the shaking lasts for 5-10 times and stops at the right limit, and then stops pumping liquid every 1-2 minutes and shakes for 5-10 times and stops at the right limit. Start the calibration process until it is completed, calculate and replace the actual pumping rate; start the subpackaging process according to the subpackaging parameters until it is completed; start the exhaust process until it is completed; stop refrigeration and shaking, close all the clamp valve structures, heat-seal (through hot melting, the pipeline 90 forms a length of tube wall fusion and then disconnects it) and removes the subpackaging bag; heat-seals and disconnects the pipeline 90 kit from the pipeline 90 of the cell suspension bag; complete, or replace a new set of pipeline 90 kits with the cell suspension bag after sterile connection and repeat the refrigeration, shaking and subpackaging processes.

[0103] The pipeline 90 and the branch pipe 160 are installed on the bracket 100, the cell suspension bag is installed in the box body 20, and the cell suspension bag 20 is connected to the pipeline 90; when the device is powered on, the temperature of the cell suspension bag in the box body 20 is controlled; the controller 70 controls the rotation of the power source 40 to drive the box body 20 to reciprocate through the transmission mechanism 50, so that the mixing movement of the cell suspension in the cell suspension bag in the box body 20 continues; the closed pipeline control device 2 is used to calibrate the peristaltic pump 120, sub-pack the shaken cell suspension and exhaust the pipeline; stop the refrigeration and shaking of the cell suspension refrigeration and shaking device 1, close all the clamp valve structures, and remove the sub-packaging bag after heat sealing; and disconnect the connection between the pipeline 90 and the cell suspension bag by heat sealing.

[0104] The above-mentioned automatic packaging device, through the cell suspension refrigeration and shaking device 1 and the closed pipeline control device 2, uses the cell suspension refrigeration and shaking device 1 to perform horizontal large-angle reciprocating oscillation of the cell suspension bag and reciprocating oscillation of the center of gravity back and forth, so as to effectively prevent the cells from sinking to the bottom and improve the mixing effect. The cell suspension bag is placed in a closed low-temperature box, and a closed-loop control method is adopted to ensure accurate temperature control and avoid the generation of condensed water. The cell activity can be effectively maintained during the large-volume and long-term packaging process. The closed pipeline control device 2 is a closed pipeline 90-type packaging method, which has low requirements for the background environment and reduces the risk of pollution; it solves the problems of high requirements for the operating environment, low packaging accuracy, poor mixing effect and inaccurate temperature control of the existing device.

[0105] The above is only a specific implementation of the utility model, but the protection scope of the utility model is not limited thereto. Any technician familiar with the technical field can easily think of various equivalent modifications or replacements within the technical scope disclosed by the utility model, and these modifications or replacements should be included in the protection scope of the utility model. Therefore, the protection scope of the utility model should be based on the protection scope of the claims.

Claims

1. An automatic packaging device, characterized in that: include: A cell suspension refrigeration and shaking device and a closed pipeline control device; wherein the cell suspension refrigeration and shaking device comprises a base, a box body, a semiconductor refrigeration structure, a power source, a transmission mechanism, a temperature sensor, a temperature controller and a controller, wherein the temperature sensor is built into the box body, and the temperature sensor is connected to the temperature controller; the temperature controller is connected to the semiconductor refrigeration structure; the transmission mechanism is connected to the semiconductor refrigeration structure; and the transmission mechanism is connected to the base; the box body is connected to the semiconductor refrigeration structure, and the controller is connected to the power source; the power source is connected to the transmission mechanism, and a cell suspension bag placed parallel to the bottom of the box body is placed in the box body; the closed pipeline control device comprises a pipeline, a bracket, a control A unit, a peristaltic pump, an air filter, a bubble sensor group and a clamping valve group, the bubble sensor group and the clamping valve group are respectively connected to the bracket, the peristaltic pump is connected to the bracket, one end of the pipeline is connected to the cell suspension bag, the other end of the pipeline passes through the peristaltic pump, the other end of the pipeline is also connected to a plurality of branch pipes, the branch pipes are connected to the sub-packaging bags, a first slot body is provided in the bubble sensor group, and the branch pipe is clamped in the first slot body; a second slot body is provided in the clamping valve group, and the branch pipe is clamped in the second slot body; the air filter is also connected to the pipeline, the control unit is respectively connected to the peristaltic pump, the bubble sensor group and the clamping valve group; the base is connected to the bracket.

2. An automatic packaging device according to claim 1, characterized in that: The box body is provided with a cavity for placing a cell suspension bag; the lower end surface of the box body is connected to the semiconductor refrigeration structure; the side wall and the upper end surface of the cavity are respectively connected to heat-insulating foam.

3. An automatic packaging device according to claim 2, characterized in that: The semiconductor refrigeration structure includes a shell with an opening at the upper end, a semiconductor refrigeration plate, a heat pipe, a heat sink and a fan; the semiconductor refrigeration plate, the heat pipe, the heat sink and the fan are respectively installed in the shell, and the opening of the shell is connected to the lower end surface of the box body.

4. An automatic packaging device according to claim 3, characterized in that: The transmission mechanism includes a first synchronous wheel, a synchronous belt, a rotating shaft and a second synchronous wheel. The power source includes a first output shaft, the first output shaft is connected to the first synchronous wheel; the second synchronous wheel is connected to the rotating shaft; the other end of the rotating shaft passes through the housing and is connected to the housing; the second synchronous wheel is connected to the first synchronous wheel through the synchronous belt.

5. The automatic packaging device according to claim 1, characterized in that: The bubble sensor group includes a first bubble sensor, the pinch valve group includes a first pinch valve structure, and the other end of the pipeline passes through the first bubble sensor and the first pinch valve structure in sequence.

6. An automatic packaging device according to claim 5, characterized in that: The bubble sensor group includes a plurality of second bubble sensors, and the pinch valve group also includes a plurality of second pinch valve structures. One end of the branch pipe is connected to the pipeline, and the other end of the branch pipe is connected to the packaging bag after passing through the second pinch valve structure and the second bubble sensor in sequence.

7. An automatic packaging device according to claim 6, characterized in that: The bubble sensor group also includes a third bubble sensor and a fourth bubble sensor, and the other end of the pipeline passes through the third bubble sensor and the fourth bubble sensor in sequence; the pinch valve group also includes a third pinch valve structure and a fourth pinch valve structure, and the other end of the pipeline passes through the third bubble sensor, the fourth bubble sensor, the third pinch valve structure, and the fourth pinch valve structure in sequence and then returns to one end of the pipeline connected to the peristaltic pump.

8. An automatic packaging device according to claim 7, characterized in that: The air filter is connected to the pipeline between the fourth air bubble sensor and the third pinch valve structure.

9. The automatic packaging device according to claim 1, characterized in that: A liquid bag outlet pipe fixing block is installed on the bracket, the other end of the branch pipe is connected to the sub-packaging bag outlet pipe, and the sub-packaging bag outlet pipe is connected to the liquid bag outlet pipe fixing block.

10. An automatic packaging device according to claim 9, characterized in that: The dispensing bag outlet pipe is connected to the dispensing bag.

Images