Secondary sterile subpackaging device

Abstract

The utility model relates to the technical field of split charging equipment, and discloses a secondary sterile split charging device which is characterized in that an equivalent splitter plate is arranged at the top in a clean operation table, a plurality of liquid outlet nozzles are arranged at the bottom of the equivalent splitter plate, the top of the equivalent splitter plate is communicated with a bottle body through a pipeline, and the equivalent splitter plate equivalently distributes samples conveyed by the pipeline to each liquid outlet nozzle; a pipe frame located below the equivalent splitter plate is arranged on the table top in the clean operation table, a plurality of fixing holes for fixing reagent pipes are formed in the pipe frame and correspond to the liquid outlet nozzles in a one-to-one mode, and the liquid outlet nozzles can change the liquid outlet direction so that samples can be attached to the pipe walls of the reagent pipes to flow down. Compared with repeated measuring, counting, split charging and direct back division of a pipette, the sample is equally divided to each liquid outlet nozzle by using the equivalent air flow plate, so that the split charging precision and efficiency are improved, manual operation is not needed, and human resources are saved; the liquid outlet nozzle changes the liquid outlet direction to enable the sample to flow down along the tube wall of the reagent tube, so that the protein activity is prevented from being influenced by bubbles, and the sample is prevented from being retained at the mouth of the reagent tube to cause pollution.

Description

Technical Field

[0001] The present application relates to the technical field of packaging equipment, and in particular to a secondary aseptic packaging device. Background Art

[0002] At present, samples are widely used in life science research, biotechnology and medicine. Especially for experimental operations involving no endotoxin, such as peptidoglycan detection products, conventional sterilization cannot destroy the cell wall of bacteria, effectively avoid the intervention of endotoxin, and seriously affect the use effect of sample reagents in the later stage. Especially in the process of equal volume packaging of large-volume bottled samples (such as hemolymph, matrix fluid, etc.) into small specifications, high sterility requirements are required, and the operation needs to be performed in a clean bench. It is necessary to hold the reagent bottle in one hand and hold tweezers in the other hand to open the bottle mouth of the bottled reagent, and then use a pipette to repeatedly measure, count and package. The operation process is cumbersome and time-consuming. It is easy to add samples to the mouth and wall of the small reagent bottle, which is easy to cause packaging contamination. The error of direct reversal is large, which affects the average packaging accuracy and effect. There is no convenient equal volume packaging system on the market that meets the experimental needs.

[0003] Therefore, how to save human resources, avoid product contamination caused by packaging, and improve packaging accuracy and efficiency are problems that technical personnel in this field need to solve. Utility Model Content

[0004] The purpose of the present application is to provide a secondary aseptic packaging device, which is used to solve the problems of repeated measurement, counting and packaging using a pipette, which is cumbersome and time-consuming, and easily causes sample addition to the mouth and wall of a small reagent bottle, which easily causes packaging contamination; and the problem of large errors in direct pouring, which affects the average packaging accuracy and effect.

[0005] In order to solve the above technical problems, the present application provides a secondary aseptic filling device, comprising: a clean operating table, an equal amount of diverter plate, a liquid outlet nozzle, a pipe rack and a pipeline;

[0006] An equal flow distribution plate is provided on the top of the clean operating table, and a plurality of liquid outlets are provided at the bottom of the equal flow distribution plate. The top of the equal flow distribution plate is connected to the bottle body containing the sample through the pipeline, and the equal flow distribution plate is used to distribute the sample transported by the pipeline to each liquid outlet in equal amounts. A tube rack located below the equal flow distribution plate is provided on the table top of the clean operating table, and a plurality of fixing holes for fixing reagent tubes are provided on the tube rack, and the fixing holes correspond to the liquid outlets one by one. The liquid outlets can change the liquid outlet direction so that the sample flows downstream along the tube wall of the reagent tube.

[0007] Optionally, a first fixing rod is provided in the clean operating table, and a fixing piece is provided on the first fixing rod, and the fixing piece is used to fix the bottle body so that the bottle mouth of the bottle body faces downward, and the bottle mouth is located above the equal amount diverter plate. The pipeline is connected to a first bottle cap, and the first bottle cap is detachably connected to the bottle mouth of the bottle body. The first bottle cap is provided with an air filter with a one-way valve.

[0008] Optionally, the pipeline is a double-layer pipeline, the inner layer is a soft tube that does not absorb proteins, and the outer layer is a metal shaped soft tube, so that the double-layer pipeline can be bent and shaped arbitrarily.

[0009] Optionally, it further includes a first clamping arm, wherein the first clamping arm is connected to the first fixing rod via a damping shaft, and the first clamping arm is used to clamp the first bottle cap.

[0010] Optionally, a gooseneck tube is further included, wherein the gooseneck tube is respectively connected to the equal-volume diverter plate and the clean operating table, and the gooseneck tube is used to suspend the equal-volume diverter plate above the pipe rack.

[0011] Optionally, it also includes a second clamping arm, a fixed frame, a rotary lifting motor and a controller, wherein the fixed frame is used to fix the bottle body, the rotary lifting motor is provided at the bottom of the fixed frame, the second clamping arm is provided in the clean operating table, and is used to clamp a second bottle cap threadedly connected to the bottle body, and the controller is used to drive the rotary lifting motor to drive the fixed frame to rotate, so that the bottle body rotates and separates from the second bottle cap.

[0012] Optionally, a second fixing rod is further included, and a clamp is provided at the end of the second clamp arm, the clamp is sleeved on the second fixing rod, and a bolt hole is provided on the clamp, and the bolt hole is used to cooperate with a bolt to fix the clamp to the second fixing rod.

[0013] Optionally, the liquid outlet nozzle is rotatably connected to the equal-volume diverter plate via a universal ball bearing.

[0014] Optionally, the liquid outlet nozzle is also provided with a proportional valve.

[0015] Optionally, a baffle is slidably connected to the opening of the side wall of the clean workbench, a support column is provided on the bottom surface of the clean workbench, a universal silent wheel with a brake device is provided on the bottom of the support column, a ventilation, disinfection and lighting system is also provided inside the clean workbench, an operation key is provided on the outside of the clean workbench, the operation key is communicatively connected with the ventilation, disinfection and lighting system, and a pull-out storage box is also provided on the bottom surface of the clean workbench.

[0016] A secondary aseptic packaging device provided by the present application includes: a clean operating table, an equal flow distribution plate, a liquid outlet nozzle, a pipe rack and a pipeline; an equal flow distribution plate is provided on the top of the clean operating table, and a plurality of liquid outlet nozzles are provided at the bottom of the equal flow distribution plate. The top of the equal flow distribution plate is connected to the bottle body containing the sample through a pipeline, and the equal flow distribution plate is used to distribute the sample transported by the pipeline to each liquid outlet nozzle in equal amounts. A pipe rack located below the equal flow distribution plate is provided on the tabletop in the clean operating table, and a plurality of fixed holes for fixing the reagent tube are provided on the pipe rack. The fixed holes correspond to the liquid outlet nozzles one by one, and the liquid outlet nozzle can change the liquid outlet direction so that the sample is close to the tube wall of the reagent tube and flows downstream. Compared with the use of a pipette gun to repeatedly measure, count and package and directly pour, the sample is divided into equal parts to each liquid outlet nozzle by using an equal flow plate, which improves the packaging accuracy and efficiency without manual operation, saving human resources; the other liquid outlet nozzle changes the liquid outlet direction so that the sample is close to the tube wall of the reagent tube and flows downstream, avoiding the generation of bubbles that affect the activity of the protein, and preventing the sample from being retained at the mouth of the reagent tube and causing pollution. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the embodiments of the present application, the following is a brief introduction to the drawings required for use in the embodiments. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0018] Figure 1 A structural diagram of a secondary aseptic filling device provided in an embodiment of the present application;

[0019] Figure 2 A partial structural diagram of a secondary aseptic filling device provided in an embodiment of the present application;

[0020] The figure numbers are as follows: 1 is a clean operating table, 2 is an equal amount diverter plate, 3 is a liquid outlet nozzle, 4 is a tube rack, 5 is a pipeline, 6 is a bottle body, 7 is a reagent tube, 8 is a first fixing rod, 9 is a fixing part, 10 is a first bottle cap, 11 is an air filter, 12 is a first clamping arm, 13 is a gooseneck tube, 14 is a second clamping arm, 15 is a fixed frame, 16 is a rotary lifting motor, 17 is a controller, 18 is a second bottle cap, 19 is a second fixing rod, 20 is an operating key, 101 is a table top, 102 is a baffle, 103 is a support column, 104 is a universal silent wheel, and 105 is a pull-out storage box. DETAILED DESCRIPTION

[0021] The following will be combined with the drawings in the embodiments of the present application to clearly and completely describe the technical solutions in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of this application.

[0022] The core of the present application is to provide a secondary aseptic filling device for saving human resources, avoiding product contamination caused by filling, and improving filling accuracy and efficiency.

[0023] In order to enable those skilled in the art to better understand the present application, the present application is further described in detail below in conjunction with the accompanying drawings and specific implementation methods.

[0024] Figure 1 A structural diagram of a secondary aseptic packaging device provided in an embodiment of the present application, such as Figure 1 As shown, the secondary aseptic filling device includes: a clean operating table 1, an equal-volume diverter plate 2, a liquid outlet nozzle 3, a tube rack 4 and a pipeline 5; an equal-volume diverter plate 2 is provided on the top of the clean operating table 1, and a plurality of liquid outlet nozzles 3 are provided at the bottom of the equal-volume diverter plate 2. The top of the equal-volume diverter plate 2 is connected to a bottle body 6 containing a sample through a pipeline 5. The equal-volume diverter plate 2 is used to distribute the sample transported by the pipeline 5 to each liquid outlet nozzle 3 in equal amounts. A tube rack 4 located below the equal-volume diverter plate 2 is provided on the table top 101 in the clean operating table 1. A plurality of fixing holes for fixing reagent tubes 7 are provided on the tube rack 4. The fixing holes correspond to the liquid outlet nozzles 3 one by one. The liquid outlet nozzle 3 can change the liquid outlet direction so that the sample flows downstream along the wall of the reagent tube 7.

[0025] The present application embodiment does not specifically limit the clean operating table 1. Specifically, Figure 1 As shown, a baffle 102 is slidably connected to the opening of the side wall of the clean operating table 1. By setting the baffle 102 that is pushed and pulled up and down to close, it is convenient for the staff to take and put the test items, and it has a protective effect; a support column 103 is provided on the bottom surface of the clean operating table 1, and a universal silent wheel 104 with a brake device is provided at the bottom of the support column 103. By setting the universal silent wheel 104 with a brake device, the entire clean operating table 1 can be conveniently transferred and fixed; a ventilation, disinfection and lighting system is also provided in the clean operating table 1, and an operation key 20 is provided on the outside of the clean operating table 1. The operation key 20 is communicated with the ventilation, disinfection and lighting system. The staff can use the operation key 20 to control the ventilation, disinfection and lighting system. The ventilation, disinfection and lighting system may include an air pump, a fluorescent lamp and an ultraviolet lamp, etc.; a pull-out storage box 105 is also provided on the bottom surface of the clean operating table 1, which is convenient for taking and temporarily storing and disinfecting consumables.

[0026] An equal-volume diverter plate 2 is provided on the top of the clean operating table 1. The embodiment of the present application does not specifically limit the arrangement of the equal-volume diverter plate 2. The equal-volume diverter plate 2 may be fixed to the top wall of the clean operating table 1, or the equal-volume diverter plate 2 may be suspended above the pipe rack 4 using a hanging part. Figure 2 A partial structural diagram of a secondary aseptic packaging device provided in an embodiment of the present application, such as Figure 2 As shown, the bottom surface of the equal-volume diverter plate 2 is provided with a plurality of liquid outlet nozzles 3, and the liquid outlet nozzles 3 may be hollow truncated cone-shaped. The plurality of liquid outlet nozzles 3 may be evenly distributed on the bottom surface of the equal-volume diverter plate 2, and there is a gap between two adjacent liquid outlet nozzles 3, so that the liquid outlet nozzles 3 correspond one-to-one with the fixed holes on the tube rack 4. The liquid outlet direction of the liquid outlet nozzle 3 is adjustable, so that the liquid sample can flow along the wall, avoiding the generation of bubbles and affecting the activity of the protein; regarding how the liquid outlet nozzle 3 changes the liquid outlet direction, the embodiments of the present application do not specifically limit this, one is that the liquid outlet nozzle 3 adopts a flexible and shaped hose, and the other liquid outlet nozzle 3 is rotatably connected to the equal-volume diverter plate 2 by a rotating part, and of course, other methods are also possible, which are not listed here one by one. The equal-volume diverter plate 2, the liquid outlet nozzle 3, the pipeline 5 and the first bottle cap 10 described below are all made of high-pressure and high-temperature resistant non-protein-adsorbing materials.

[0027] One end of the pipe 5 is connected to the bottle body 6 containing the sample, and the other end is connected to the equal-volume diversion plate 2. As to how to use the pipe 5 to transport the sample in the bottle body 6 to the equal-volume diversion plate 2, one method is to set a water pump on the pipe 5, and the other method is to invert the bottle body 6 and set it above the equal-volume diversion plate 2, and use gravity to make the sample flow into the equal-volume diversion plate 2 through the pipe 5.

[0028] The pipe rack 4 can be directly placed on the table 101 in the clean operating table 1, or positioning pins can be set on the table 101, and the pipe rack 4 is placed in a positioning groove formed by a plurality of positioning pins to play a role of fixing and positioning.

[0029] A secondary aseptic filling device provided in an embodiment of the present application includes: a clean operating table, an equal flow distribution plate, a liquid outlet nozzle, a tube rack and a pipeline; an equal flow distribution plate is provided on the top of the clean operating table, and a plurality of liquid outlet nozzles are provided at the bottom of the equal flow distribution plate. The top of the equal flow distribution plate is connected to a bottle body containing samples through a pipeline. The equal flow distribution plate is used to distribute the samples transported by the pipeline to each liquid outlet nozzle in equal amounts. A tube rack located below the equal flow distribution plate is provided on the table top of the clean operating table. The tube rack is provided with a plurality of fixing holes for fixing reagent tubes. The fixing holes correspond to the liquid outlet nozzles one by one. The liquid outlet nozzle can change the liquid outlet direction so that the sample flows downstream along the tube wall of the reagent tube. Compared with the use of a pipette to repeatedly measure, count and dispense, and directly pour the sample, the equal volume air flow plate is used to divide the sample equally into each liquid outlet, which improves the packaging accuracy and efficiency while eliminating the need for manual operation and saving human resources. In addition, the liquid outlet changes the direction of the liquid so that the sample flows along the wall of the reagent tube, avoiding the generation of bubbles that affect protein activity and preventing the sample from accumulating at the mouth of the reagent tube and causing contamination.

[0030] Based on the above embodiments, a first fixing rod 8 is provided in the clean operating table 1 of the embodiment of the present application, and a fixing member 9 is provided on the first fixing rod 8. The fixing member 9 is used to fix the bottle body 6 so that the bottle mouth of the bottle body 6 faces downward, and the bottle mouth is located above the equal amount diverter plate 2. The pipeline 5 is connected to a first bottle cap 10, and the first bottle cap 10 is detachably connected to the bottle mouth of the bottle body 6. The first bottle cap 10 is provided with an air filter 11 with a one-way valve.

[0031] The embodiment of the present application uses gravity to transport samples to the equal amount of diverter plate 2. Compared with using a water pump to transport samples, it can save equipment costs. By setting an air filter 11 with a one-way valve, while balancing the air pressure so that the sample in the bottle body 6 flows smoothly into the pipeline 5, it can also prevent the sample from being contaminated and ensure sterility. The first bottle cap 10 in the embodiment of the present application is connected to the pipeline 5, and a sealing ring can be set at the connection. Regarding the connection mode of the first bottle cap 10 and the bottle body 6, it can be a clamping connection or a threaded connection. In order to prevent the sample from leaking, the threaded connection mode is preferred. Regarding the connection mode of the fixing member 9 and the first fixing rod 8, it can be a fixed connection or a detachable connection mode; in order to facilitate the adjustment of the height of the bottle body 6, the fixing member 9 is height-adjustable on the first fixing rod 8. Specifically, the length direction of the first fixing rod 8 is provided with a bolt hole, and the fixing member 9 is provided with a bolt. One of the bolts is selected and connected with the bolt hole to change the height of the fixing member 9; of course, other height-adjustable connection modes can also be used. Among them, the first bottle cap 10 and the pipeline 5 are preferably detachably connected to adapt to bottle mouths of different specifications.

[0032] Based on the above embodiment, the embodiment of the present application further includes a first clamping arm 12 , which is connected to the first fixing rod 8 via a damping shaft, and the first clamping arm 12 is used to clamp the first bottle cap 10 .

[0033] The first clamp arm 12 is connected by a damping shaft, the damping shaft is arranged horizontally, the first clamp arm 12 can rotate around the damping shaft, and the first clamp arm 12 can be a clamp, which is used to clamp the first bottle cap 10 so that the first bottle cap 10 is suspended in the air to prevent contamination. At the same time, when the first bottle cap 10 is connected to the bottle body 6, it can play a certain fixing role to prevent the first bottle cap 10 from being separated from the bottle body 6. In addition, the first clamp arm 12 can also be arranged on the first fixing rod 8 in a height-adjustable manner. For specific methods, please refer to the method in which the fixing member 9 is arranged on the first fixing rod 8 in a height-adjustable manner as described above.

[0034] Based on the above embodiments, the pipe 5 of the embodiment of the present application is a double-layer pipe, the inner layer is a soft tube that does not absorb proteins, and the outer layer is a metal shaped soft tube, so that the double-layer pipe can be bent and shaped arbitrarily.

[0035] The inner tube of the embodiment of the present application is made of a material that does not absorb protein, which is easy to clean and reuse, and has little effect on the sample. The material that does not absorb protein includes soluble polytetrafluoroethylene (PFA), fluorinated ethylene propylene (FEP), etc., which meet the high requirements of experimental conditions such as removing endotoxins, and can withstand high and low temperatures. The operating temperature can reach -200 to 260°C; the outer tube is a metal shaped hose. The above materials determine that the double-layer pipe can be bent and shaped at will. Since the double-layer pipe can be bent and shaped at will, it is convenient to adjust with the position change of the bottle body 6, which provides convenience for the staff's operation.

[0036] Based on the above embodiments, the embodiments of the present application further include a gooseneck 13 , which is respectively connected to the equal-volume diverter plate 2 and the clean operating table 1 , and is used to suspend the equal-volume diverter plate 2 above the pipe rack 4 .

[0037] like Figure 1 As shown, in order to stably suspend the equal-volume diverter plate 2, two gooseneck tubes 13 are provided. One end of the gooseneck tube 13 is fixedly connected to the side wall of the clean operating table 1, and the other end is connected to the equal-volume diverter plate 2. The gooseneck tube 13 is a metal shaped hose, which is a metal part that can be bent arbitrarily and determine the direction. Therefore, it is convenient for the staff to adjust the position of the equal-volume diverter plate 2 as needed, so that the liquid outlet nozzle 3 can correspond one-to-one with the reagent tube 7, thereby ensuring that the sample accurately flows into the reagent tube 7.

[0038] Based on the above embodiments, the embodiments of the present application also include a second clamping arm 14, a fixed frame 15, a rotating lifting motor 16 and a controller 17. The fixed frame 15 is used to fix the bottle body 6. A rotating lifting motor 16 is provided at the bottom of the fixed frame 15. The second clamping arm 14 is provided in the clean operating table 1 and is used to clamp the second bottle cap 18 threadedly connected to the bottle body 6. The controller 17 is used to drive the rotating lifting motor 16 to drive the fixed frame 15 to rotate so that the bottle body 6 rotates and separates from the second bottle cap 18.

[0039] The rotary lifting motor 16 is arranged on the table 101 of the clean operating table 1. The second clamp arm 14 can be a clamp, which is used to clamp the second bottle cap 18 on the bottle body 6. In order to clamp the second bottle cap 18, the distance between the two clamping parts of the second clamp arm 14 is adjustable. Specifically, the ends of the two clamping parts 18 can be provided with bolt holes, and the bolt holes cooperate with the bolts so that the two clamping parts clamp the second bottle cap 18. At the same time, this design can also be adapted to bottle caps of different specifications. Further, considering the different heights of the bottle body 6, in order to enable the second clamp arm 14 to clamp the second bottle cap 18, it also includes a second fixing rod 19, and the end of the second clamp arm 14 is provided with a clamp, and the clamp is sleeved on the second fixing rod 19, and the clamp is provided with a bolt hole, and the bolt hole is used to cooperate with the bolt to fix the clamp to the second fixing rod 19. Through this design, the height of the second clamp arm 14 can be adjusted, so as to adapt to bottles 6 of different heights. The fixed frame 15 can be rectangular. Whether the bottle body 6 is rectangular or cylindrical, when it is in the fixed frame 15, the fixed frame 15 can limit the bottle body 6 to prevent the bottle body 6 from rotating relative to the fixed frame 15. Based on this, the rotary lifting motor 16 at the bottom of the fixed frame 15 drives the fixed frame 15 to rotate and descend, and the bottle body 6 also rotates and descends synchronously. Since the second bottle cap 18 is connected to the bottle body 6 by a thread, the second clamping arm 14 clamps the second bottle cap 18, and the bottle body 6 is separated from the second bottle cap 18. The above-mentioned setting can replace the manual unscrewing of the bottle cap, reduce the contact between people and the bottle cap, avoid pollution, and one-button operation, which is safe and fast. Further, the fixed frame 15 can be detachably connected to the rotary lifting motor 16, which is convenient for replacing fixed frames 15 of different specifications to adapt to different models of bottle bodies 6.

[0040] Based on the above embodiment, regarding how the liquid outlet nozzle 3 adjusts the liquid outlet direction, the liquid outlet nozzle 3 may be rotatably connected to the equal-volume splitter plate 2 via a universal ball bearing. It should be noted that the equal-volume splitter plate 2, the universal ball bearing and the liquid outlet nozzle 3 are in a connected state.

[0041] Furthermore, in order to better adjust the flow rate of the sample and select the average packaging quantity, the liquid outlet nozzle 3 is also provided with a proportional valve. Of course, other methods are also possible, for example, the liquid outlet nozzle 3 is provided with a baffle to block the opening size of the liquid outlet. By controlling the flow rate of the sample, it is prevented that the sample flows too fast and splashes out of the reagent tube 7 to cause pollution and inaccurate packaging.

[0042] For ease of understanding, the following describes the use of the secondary aseptic packaging device, and disinfects and sterilizes the components in the clean operating table 1; then place the bottle body 6 of the matrix liquid to be packaged in the fixed frame 15, fix the second bottle cap 18 by the second clamp arm 14, and then start the controller 17 to fall off the second bottle cap 18, and the operation of falling off the second bottle cap 18 can also be completed manually; then adjust the position of the first clamp arm 12 and the pipeline 5, transfer the first bottle cap 10 to just above the bottle body 6, start the controller 17, the fixed frame 15 reversely rotates and rises, the bottle mouth and the first bottle cap 10 are just spirally connected and sealed, and the operation of connecting the first bottle cap 10 to the bottle body 6 can also be completed manually; put the corresponding reagent tube 7 on the tube rack 4, and after the liquid outlet nozzle 3 and the reagent tube 7 are matched one by one, adjust the liquid outlet direction of the liquid outlet nozzle 3, and then adjust the direction of the bottle body 6 of the large bottle of matrix liquid to be inverted, and the bottle body is clamped in the clamping fixture 9, so that semi-automatic and efficient equal-volume secondary packaging of sterile sample reagents can be achieved. Repeat the above steps until all large bottles of live materials have been packaged. After disassembling the components for cleaning and disinfection, sterilize them at high temperature before the next use to remove endotoxins and then reuse them to save resources.

[0043] The secondary aseptic packaging device of the present application has a simple structure and is easy to operate. It not only realizes the efficient secondary packaging experiment of aseptic bottled live materials, but also avoids the risk of product contamination caused by consumables. The packaging process can carry out large-scale continuous aseptic packaging, and the operation is more convenient. It avoids the disadvantages of arm soreness and thumb numbness caused by long-term holding of the sample gun by the experimenter, which is beneficial to the health of the operator. The auxiliary system is not only convenient and safe to transfer and fix, but also safe and efficient. The operating parts are easy to reuse after sterilization, saving resources. It can be packaged by connecting the bottle mouth of the large bottle of live materials, and the chance of the live materials contacting the outside world is smaller, which further ensures the sterility and safety of the experiment, and is of great help to the existing experimental links.

[0044] The above is a detailed introduction to a secondary aseptic filling device provided by the present application. The various embodiments in the specification are described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same and similar parts between the various embodiments can be referenced to each other. It should be pointed out that for ordinary technicians in this technical field, without departing from the principles of the present application, several improvements and modifications can be made to the present application, and these improvements and modifications also fall within the scope of protection of the claims of the present application.

[0045] It should also be noted that, in this specification, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "comprise", "include" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of further restrictions, an element defined by the statement "comprises a ..." does not exclude the presence of other identical elements in the process, method, article or device including the element.

Claims

1. A secondary aseptic packaging device, characterized in that: include: A clean operating table (1), an equal-volume manifold (2), a liquid outlet nozzle (3), a pipe rack (4) and a pipe (5); An equal flow distribution plate (2) is provided at the top of the clean operating table (1), and a plurality of liquid outlet nozzles (3) are provided at the bottom of the equal flow distribution plate (2). The top of the equal flow distribution plate (2) is connected to a bottle (6) containing a sample through the pipeline (5). The equal flow distribution plate (2) is used to distribute the sample transported by the pipeline (5) to each liquid outlet nozzle (3) in equal amounts. A tube rack (4) located below the equal flow distribution plate (2) is provided on the table top (101) in the clean operating table (1). A plurality of fixing holes for fixing reagent tubes (7) are provided on the tube rack (4), and the fixing holes correspond to the liquid outlet nozzles (3) one by one. The liquid outlet nozzle (3) can change the liquid outlet direction so that the sample flows downstream along the tube wall of the reagent tube (7); wherein the liquid outlet nozzle (3) adopts a flexible and shaped hose.

2. The secondary aseptic filling device according to claim 1, characterized in that: A first fixing rod (8) is provided in the clean operating table (1), and a fixing member (9) is provided on the first fixing rod (8). The fixing member (9) is used to fix the bottle body (6) so that the bottle mouth of the bottle body (6) faces downward, and the bottle mouth is located above the equal-volume diverter plate (2). The pipeline (5) is connected to a first bottle cap (10), and the first bottle cap (10) is detachably connected to the bottle mouth of the bottle body (6). The first bottle cap (10) is provided with an air filter (11) with a one-way valve.

3. The secondary aseptic packaging device according to claim 1, characterized in that: The pipeline (5) is a double-layer pipeline, the inner layer is a soft tube that does not absorb protein, and the outer layer is a metal shaped soft tube, so that the double-layer pipeline can be bent and shaped at will.

4. The secondary aseptic packaging device according to claim 2, characterized in that: It also comprises a first clamping arm (12), wherein the first clamping arm (12) is connected to the first fixing rod (8) via a damping shaft, and the first clamping arm (12) is used to clamp the first bottle cap (10).

5. The secondary aseptic packaging device according to claim 1, characterized in that: It also includes a gooseneck tube (13), which is connected to the equal-volume splitter plate (2) and the clean operating table (1) respectively, and the gooseneck tube (13) is used to suspend the equal-volume splitter plate (2) above the pipe rack (4).

6. The secondary aseptic filling device according to claim 1, characterized in that: The invention also comprises a second clamping arm (14), a fixed frame (15), a rotary lifting motor (16) and a controller (17); the fixed frame (15) is used to fix the bottle body (6); the rotary lifting motor (16) is provided at the bottom of the fixed frame (15); the second clamping arm (14) is provided in the clean operating table (1) and is used to clamp a second bottle cap (18) threadedly connected to the bottle body (6); the controller (17) is used to drive the rotary lifting motor (16) to drive the fixed frame (15) to rotate, so that the bottle body (6) is rotated and separated from the second bottle cap (18).

7. The secondary aseptic filling device according to claim 6, characterized in that: It also comprises a second fixing rod (19), the end of the second clamp arm (14) is provided with a clamping member, the clamping member is sleeved on the second fixing rod (19), the clamping member is provided with a bolt hole, the bolt hole is used to cooperate with a bolt to fix the clamping member on the second fixing rod (19).

8. The secondary aseptic filling device according to claim 1, characterized in that: The liquid outlet nozzle (3) is also provided with a proportional valve.

9. The secondary aseptic packaging device according to any one of claims 1 to 8, characterized in that: A baffle (102) is slidably connected to the opening of the side wall of the clean operating table (1), a support column (103) is provided on the bottom surface of the clean operating table (1), and a universal silent wheel (104) with a brake device is provided at the bottom of the support column (103). A ventilation, disinfection and lighting system is also provided inside the clean operating table (1), and an operation key (20) is provided outside the clean operating table (1), and the operation key (20) is communicatively connected with the ventilation, disinfection and lighting system. A pull-out storage box (105) is also provided on the bottom surface of the clean operating table (1).

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