Reagent storage assembly and kit

Abstract

The utility model relates to the field of medical instruments, in particular to a reagent storage assembly. A first main body with a cylindrical structure and a second main body with a three-dimensional structure are arranged, a plurality of first cavities are formed in the first main body, and first through holes and second through holes which are matched with the first cavities in number and communicate with the first cavities are formed in the bottom face and the top face of the first main body correspondingly. The distances between the first through holes and the axis of the cylinder structure are equal. A blind hole matched with the first body is formed in the top face of the second body, and a third through hole is formed in the bottom face of the blind hole. During use, the first main body is placed in the blind hole, the third through hole is connected with an external air source, and the first through hole corresponding to the first cavity in which the liquid to be used currently is located can be communicated with the external air source by rotating the first main body to enable the first through hole to correspond to the third through hole in position, so that manual pipetting operation is not needed, and the operation efficiency is improved; the technical problem that in the prior art, efficiency is low due to the fact that liquid relief depends on manual operation is solved.

Description

Technical Field

[0001] The utility model relates to the field of medical equipment, in particular to a reagent storage component and a reagent kit. Background Art

[0002] Protein chip is a high-throughput protein function analysis technology that can be used for protein expression spectrum analysis and the study of protein-protein interactions. The research object of protein chip technology is protein. Its principle is to perform special chemical treatment on the solid phase carrier, and then fix the known protein molecule products on it (such as enzymes, antigens, antibodies, receptors, ligands, cytokines, etc.). According to the characteristics of these biological molecules, the test protein (present in serum, plasma, lymph, interstitial fluid, urine, exudate, cell lysate, secretion fluid, etc.) that can specifically bind to them is captured, washed, purified, and then confirmed and biochemically analyzed; it provides strong technical support for obtaining important life information. Protein chip technology has potential broad application prospects in the medical field. Protein chips can simultaneously detect the content of all proteins in biological samples that may be related to a certain disease or environmental factor damage, which is also of great significance for monitoring the process or prediction of the disease and judging the effect of treatment. At present, the detection process of protein chips is complicated, and the reagent pipetting is manually operated, which is inefficient. Utility Model Content

[0003] The utility model provides a reagent storage component, which solves the technical problem of low efficiency caused by manual operation of liquid transfer in the prior art.

[0004] In order to solve the above technical problems, the technical solution adopted in this embodiment is as follows:

[0005] In an embodiment of the first aspect, the utility model provides a reagent storage assembly, comprising:

[0006] A first body, wherein the first body has a columnar structure; a plurality of first chambers are arranged in the first body; a plurality of first through holes and a plurality of second through holes are respectively opened on the bottom surface and the top surface of the first body, the plurality of first through holes and the plurality of second through holes being matched with the number of the first chambers; the plurality of first through holes and the plurality of second through holes are respectively connected with the plurality of first chambers; the plurality of first through holes are equidistant from the axis of the columnar structure;

[0007] The second body has a three-dimensional structure, a blind hole is formed on the top surface of the second body, the size of the blind hole matches that of the first body, and a third through hole is formed on the bottom surface of the blind hole; the first through hole can correspond to the position of the third through hole.

[0008] In some embodiments, the reagent storage assembly further comprises:

[0009] A first sealing film; the first sealing film is sealed and connected to the bottom surface of the first body to seal the first through holes;

[0010] An ejector plate, whose size matches the blind hole, and whose surface has a plurality of protrusions; the protrusions match the shape and size of the second through hole; a plurality of the protrusions respectively correspond to the positions of a plurality of the second through holes; a plurality of the protrusions respectively have a sixth through hole that passes through the top end thereof and the bottom surface of the ejector plate, and the sixth through hole can correspond to the position of the third through hole; the ejector plate is placed at the bottom of the blind hole, and the reagent storage assembly is placed on the ejector plate.

[0011] In some embodiments, the reagent storage assembly further includes a second sealing film; the second sealing film is sealingly connected to the top surface of the first body to seal the plurality of second through holes.

[0012] In some embodiments, a first hole is formed on the top surface of the first body, and a cross section of the first hole is a polygon, an ellipse, or an irregular shape.

[0013] In an embodiment of the second aspect, the utility model provides a kit comprising:

[0014] The reagent storage assembly as described above; the second body further has a first groove on the top surface and a second groove on the bottom surface; the third through hole connects the blind hole with the second groove, and a fourth through hole is formed between the first groove and the second groove;

[0015] A panel, the bottom surface of which is sealedly connected to the top surface of the second body, the bottom surface of the panel and the first groove forming a second chamber; a fifth through hole is formed on the top surface of the panel, and the fifth through hole is connected to the second chamber;

[0016] A bottom plate, the top surface of which is provided with a protein coating position, on which a preset protein molecule product is fixed; the top surface of the bottom plate is sealedly connected to the bottom surface of the second body, the top surface of the bottom plate and the second groove form a third chamber, and the protein coating position is located in the third chamber.

[0017] In some embodiments, the first groove includes a liquid flow area and a liquid buffer area, and the cross-sectional area of ​​the liquid flow area is smaller than the cross-sectional area of ​​the liquid buffer area.

[0018] In some embodiments, the first groove further includes a waste liquid storage area, and the distance between the bottom of the waste liquid storage area and the top surface of the second body is greater than the distance between the bottom of the liquid flow area and the liquid buffer area and the top surface of the second body.

[0019] In some embodiments, the kit further comprises:

[0020] Sealing gasket; the sealing gasket is made of elastic material; the sealing gasket has a seventh through hole penetrating its top surface and bottom surface, and the seventh through hole corresponds to the position of the third through hole; the sealing gasket is located between the ejector plate and the bottom surface of the blind hole, and is fixedly connected to the bottom surface of the blind hole.

[0021] In some embodiments, at least two protrusions are provided on the side wall of the blind hole, and the edge of the ejector plate is provided with a first notch corresponding to the number of the protrusions and matching the shape and position; the bottom surface of the first body has a circular flange; the edge of the flange is provided with a second notch corresponding to the number of the protrusions and matching the shape and position; when the first body is pressed downward to cause the sealing gasket to deform, the first body is rotated along the axis of the first body, and the upper surface of the flange abuts against the lower surface of the protrusion.

[0022] In some embodiments, an eighth through hole is opened in the middle of the sealing gasket, and a ninth through hole corresponding to the position of the eighth through hole is opened in the middle of the ejector plate.

[0023] Beneficial Effects

[0024] When the utility model is used, the first main body is placed in the blind hole, and the third through hole is connected to the external air source. By rotating the first main body so that the positions of the first through hole and the third through hole correspond to each other, the first through hole corresponding to the first cavity where the liquid currently to be used is located can be connected to the external air source. There is no need for manual pipetting operations, which improves the operating efficiency and solves the technical problem of low efficiency caused by relying on manual pipetting operations in the prior art.

[0025] Additional aspects and advantages of the embodiments of the present invention will be given in part in the following description, and in part will become apparent from the following description, or will be understood through the practice of the embodiments of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings required for use in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0027] The methods, systems and / or programs in the accompanying drawings will be further described according to exemplary embodiments. These exemplary embodiments will be described in detail with reference to the drawings. These exemplary embodiments are non-limiting exemplary embodiments, wherein example numbers represent similar mechanisms in the various views of the accompanying drawings.

[0028] Figure 1It is a structural schematic diagram of a first main body provided by an embodiment of the utility model.

[0029] Figure 2 It is a top view of an assembled first main body provided by an embodiment of the utility model.

[0030] Figure 3 It is a longitudinal cross-sectional view of a reagent storage assembly provided in one embodiment of the utility model.

[0031] Figure 4 It is a schematic diagram of the bottom structure of the first main body provided by an embodiment of the utility model.

[0032] Figure 5 It is a structural schematic diagram of a reagent storage assembly provided in one embodiment of the utility model.

[0033] Figure 6 It is a schematic diagram of the second main structure provided by an embodiment of the utility model.

[0034] Figure 7 It is a schematic diagram of the bottom structure of the second main body provided by an embodiment of the utility model.

[0035] Figure 8 It is an exploded view of a test kit provided in one embodiment of the utility model.

[0036] Description of reference numerals:

[0037] 100-reagent storage assembly; 110-first body; 111-first chamber; 112-first through hole; 113-second through hole; 114-flange; 115-second notch; 120-first sealing film; 130-second sealing film; 140-first hole; 210-second body; 211-blind hole; 212-first groove; 2121-liquid flow area; 2122-liquid buffer area; 2123 -waste liquid storage area; 213-second groove; 214-third through hole; 215-fourth through hole; 216-bump; 220-panel; 221-fifth through hole; 230-bottom plate; 231-protein coating position; 300-thrust plate; 310-protrusion; 311-sixth through hole; 320-first notch; 330-ninth through hole; 400-sealing pad; 410-seventh through hole; 420-eighth through hole. DETAILED DESCRIPTION

[0038] In order to make the purpose, technical scheme and advantages of the embodiment of the utility model clearer, the technical scheme in the embodiment of the utility model will be clearly and completely described below in conjunction with the drawings in the embodiment of the utility model. Obviously, the described embodiment is a part of the embodiment of the utility model, rather than all the embodiments. The components of the embodiment of the utility model generally described and shown in the drawings here can be arranged and designed in various different configurations. Therefore, the following detailed description of the embodiment of the utility model provided in the drawings is not intended to limit the scope of the utility model for protection, but merely represents the selected embodiment of the utility model. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without making creative work belong to the scope of protection of the utility model.

[0039] It should be noted that similar reference numerals and letters denote similar items in the following drawings, and therefore, once an item is defined in one drawing, further definition and explanation thereof is not required in subsequent drawings.

[0040] In the description of the present utility model, it should be noted that the terms "first", "second", "third", etc. are only used to distinguish the description and cannot be understood as indicating or implying relative importance.

[0041] In the description of the present invention, it is also necessary to explain that, unless otherwise clearly specified and limited, the term "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a direct connection, or an indirect connection through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0042] See also Figure 1 Figure 6In an embodiment of the first aspect, the utility model provides a reagent storage assembly 100, including a first body 110 and a second body 210. The first body 110 has a columnar structure, and a plurality of first chambers 111 are arranged in the first body 110. The bottom surface and the top surface of the first body 110 are respectively provided with first through holes 112 and second through holes 113 matching the number of the plurality of first chambers 111. The plurality of first through holes 112 are respectively connected to the plurality of first chambers 111, and the plurality of second through holes 113 are also respectively connected to the plurality of first chambers 111. The plurality of first through holes 112 are equidistant from the axis of the columnar structure. The second body 210 has a three-dimensional structure, and a blind hole 211 is opened on its top surface, and a third through hole 214 is opened on the bottom surface of the blind hole 211. The first through hole 112 can correspond to the position of the third through hole 214. When in use, the first body 110 can be placed in the blind hole 211, the positions of the first through hole 112 and the third through hole 214 can be staggered, and the first body 110 can be pressed down so that the bottom surface of the first body 110 is in close contact with the bottom surface of the blind hole 211, and the first through hole is sealed. The sample liquid to be detected, the cleaning liquid and the reagent are respectively injected into the first chambers 111 through the second through holes 113, and the third through hole 214 is connected to the external air source. Then, the first body 110 is kept in the pressed state and rotated so that the first through hole 112 corresponding to the first chamber 111 where the liquid currently needs to be used corresponds to the third through hole 214, so that the first through hole 112 is connected to the external air source. Start the air source to extract the liquid therein, and then rotate the first body 110 around the axis so that the first through hole 112 corresponding to the first chamber 111 where the liquid to be used next is located corresponds to the third through hole 214, and start the air source again to extract the liquid therein. The utility model can switch the first chamber 111 storing different liquids to be connected to the external gas source by rotating the first body 110, without manual pipetting operation, thereby improving the operation efficiency and solving the technical problem of low efficiency caused by relying on manual pipetting in the prior art.

[0043] See also Figure 3 and Figure 5In some embodiments, the reagent storage assembly 100 further includes a first sealing film 120 and an ejector plate 300. The first sealing film 120 is sealed and connected to the bottom surface of the first body 110 to seal the plurality of first through holes 112. The size of the ejector plate 300 matches the blind hole 211, and a plurality of protrusions 310 are provided on its surface. The protrusions 310 match the shape and size of the second through holes 113. The plurality of protrusions 310 correspond to the positions of the plurality of second through holes 113, respectively. The plurality of protrusions 310 respectively have a sixth through hole 311 that runs through the top end thereof and the bottom surface of the ejector plate 300, and the sixth through hole 311 can correspond to the position of the third through hole 214. When in use, the ejector plate 300 is placed at the bottom of the blind hole 211, the first body 110 is placed on the ejector plate 300, and the plurality of protrusions 310 are respectively inserted into the plurality of first through holes 112 to pierce the first sealing film 120. The first body is rotated to align the first through hole 112 corresponding to the first chamber 111 where the liquid currently to be used is located with the third through hole 214 , and the liquid in the first chamber 111 can be extracted by starting the gas source.

[0044] Specifically, the first sealing film 120 can be sealed and connected to the bottom surface of the first body 110 by gluing or hot melting. After the first through hole 112 is sealed, commonly used reagents or cleaning solutions can be pre-stored in the first chamber 111, and the sample liquid to be tested can be added to the reserved idle first chamber 111 when it is used.

[0045] See also Figure 1 In some embodiments, the reagent storage assembly 100 further includes a second sealing film 130, which is sealed and connected to the top surface of the first body 110 to seal the plurality of second through holes 113. Specifically, the second sealing film 130 can be sealed and connected to the top surface of the first body 110 by gluing or hot melting. During use, the operator can tear off the second sealing film 130, or pierce the second sealing film 130 at the second through hole 113 to ensure that the first chamber 111 is connected to the outside, so that the external gas source can extract the liquid in the first chamber 111. The first sealing film 120 can prevent contaminants from entering the first chamber 111 from the second through hole 113, so that the reagent storage assembly 100 can be stored after pre-installing reagents or cleaning fluids.

[0046] See also Figure 2 and Figure 3In some embodiments, a first hole 140 is formed on the top surface of the first body 110, and the cross section of the first hole 140 is one of a polygon, an ellipse or an irregular shape. When in use, the operator can use an internal angle wrench, an internal angle screwdriver or other rotating device that matches the shape and size of the first hole 140 to complete the rotation operation of the reagent storage assembly 100. Preferably, the centroid of the first hole 140 and the axis of the first body 110 coincide with the intersection of the top surface of the first body 110, and its cross section is a hexagon.

[0047] See also Figures 6 to 8 In an embodiment of the second aspect, the utility model provides a reagent kit, comprising the reagent storage assembly 100, a panel 220 and a bottom plate 230 provided in the embodiment of the first aspect. Among them, the top surface of the second body 210 is also provided with a first groove 212, and the bottom surface thereof is provided with a second groove 213. The third through hole 214 connects the blind hole 211 with the second groove 213, and a fourth through hole 215 is provided between the first groove 212 and the second groove 213. The bottom surface of the panel 220 is sealedly connected to the top surface of the second body 210, and the bottom surface of the panel 220 and the first groove 212 form a second chamber. The top surface of the panel 220 is provided with a fifth through hole 221, and the fifth through hole 221 is connected to the second chamber. The top surface of the bottom plate 230 is provided with a protein coating position 231, and a preset protein molecule product is fixed on the protein coating position 231. The top surface of the bottom plate 230 is sealed and connected to the bottom surface of the second body 210, and the top surface of the bottom plate 230 and the second groove 213 form a third chamber. The protein coating position 231 is located in the third chamber. When in use, the first through hole 112 corresponding to the first chamber 111 where the liquid currently needs to be used is connected to the third through hole 214, and the fifth through hole 221 is connected to the external air source. The external air source is started for extraction operation, and the liquid can enter the third chamber from the first chamber 111 through the first through hole 112 and the third through hole 214 in sequence, and react with the preset protein molecule product or clean it after reaching the protein coating position 231. After the reaction or cleaning is completed, the air source is started to continue extraction, and the liquid continues to enter the second chamber through the fourth through hole 215. The extraction time of the air source can be adjusted according to the amount of liquid in the first chamber 111, the capacity of the second chamber and the third chamber, etc., to ensure that the liquid can reach the predetermined area.

[0048] Preferably, the bottom plate 230 may be made of a transparent material to facilitate optical inspection and avoid the need to disassemble the bottom plate 230 for optical inspection operations, thereby improving inspection efficiency.

[0049] See also Figure 6In some embodiments, the first groove 212 includes a liquid flow area 2121 and a liquid buffer area 2122. The cross-sectional area of ​​the liquid flow area 2121 is smaller than the cross-sectional area of ​​the liquid buffer area 2122. The liquid flow area 2121 is located between the liquid buffer area 2122 and the fourth through hole 215. The liquid entering the second chamber from the third chamber can be temporarily stored in the liquid buffer area 2122 after flowing through the liquid flow area 2121. In some application scenarios, in order to make the sample liquid or reagent to be tested fully react with the preset protein molecule product, or to improve the cleaning effect, the gas source can be started to repeatedly extract and inject gas, so that the liquid flows back and forth between the protein coating position 231 and the liquid buffer area 2122. Compared with the static method used in the prior art, the present invention has high mixing efficiency, can shorten the inspection time, reduce the amount of reagents used, and reduce costs.

[0050] Please continue reading Figure 6 Furthermore, the first groove 212 also includes a waste liquid storage area 2123, and the distance between the bottom of the waste liquid storage area 2123 and the top surface of the second body 210 is greater than the distance between the bottom of the liquid flow area 2121 and the liquid buffer 2122 and the top surface of the second body 210. When in use, the sample liquid, cleaning liquid or reagent flows back and forth between the protein coating position 231 and the liquid buffer until the reaction or cleaning operation is completed, and the gas source is started to suck the liquid into the waste liquid storage area 2123. Since the bottom of the waste liquid storage area 2123 is farther from the top surface of the second main body 210 than the bottom of the liquid flow area 2121 and the liquid buffer zone 2122, and the top surface of the protein carrier assembly 200 is facing upward and the bottom surface is facing downward when in use, when the liquid enters the waste liquid storage area 2123 and the gas source is subsequently started to perform the gas injection operation, the liquid in the waste liquid storage area 2123 is blocked by the side wall of the waste liquid storage area 2123 and will not flow to the liquid buffer zone 2122 again, thereby preventing the liquid in the waste liquid storage area 2123 from flowing back and affecting the subsequent reaction or cleaning process.

[0051] See also Figure 8 In some embodiments, the reagent kit further includes a sealing gasket 400, which is made of elastic material. The sealing gasket 400 has a seventh through hole 410 that runs through its top and bottom surfaces, and the seventh through hole 410 corresponds to the position of the third through hole 214. The sealing gasket 400 is located between the ejector plate 300 and the bottom surface of the blind hole 211, and is fixedly connected to the bottom surface of the blind hole 211. When in use, the reagent storage assembly 100 is pressed down to deform the sealing gasket 400. The reaction force of the sealing gasket 400 makes the top surface of the ejector plate 300 and the bottom surface of the first body 110 contact more closely, thereby improving the sealing effect between the two and preventing the liquid from flowing out of the gap between the bottom surface of the first body 110 and the top surface of the ejector plate 300.

[0052] Specifically, the sealing pad 400 can be made of materials such as silicone and rubber, and has good biocompatibility.

[0053] See also Figure 8 Furthermore, an eighth passage 420 is opened in the middle of the sealing gasket 400 to provide space for the deformation of the sealing gasket 400.

[0054] Please continue reading Figure 8 Furthermore, a ninth through hole 330 corresponding to the position of the eighth through hole 420 is opened in the middle of the ejector plate 300 to further provide space for the deformation of the sealing gasket 400.

[0055] See also Figure 8 , further, at least two protrusions 216 are provided on the side wall of the blind hole 211. The edge of the ejector plate 300 is provided with a first notch 320 corresponding to the number of the protrusions 216 and matching in shape. The bottom surface of the first body 110 has a circular flange 114, and the edge of the flange 114 is provided with a second notch 115 corresponding to the number of the protrusions 216 and matching in shape. When the first body 110 is pressed down to cause the sealing gasket 400 to deform, the first body 110 is rotated along the axis of the first body 110, and the upper surface of the flange 114 abuts against the lower surface of the protrusion 216. When in use, the protrusion 216 can continuously maintain the downward pressure state of the first body 110, avoiding the instability of the external force used to press down the first body 110 to affect the sealing effect between the bottom surface of the first body 110 and the top surface of the ejector plate 300. Preferably, the number of protrusions 216 is three, which can further maintain the compression state of the ejector plate 300.

[0056] In a preferred embodiment, the operator can operate the kit to complete the immunofluorescence biological detection of the sample to be detected through the following steps:

[0057] The second sealing mold is torn open, and the first chambers 111 are pre-installed with the first cleaning liquid, the secondary antibody reagent, the second cleaning liquid, the fluorescent reagent, and the third cleaning liquid in a counterclockwise direction. The first chamber 111 in front of the first chamber 111 storing the first cleaning liquid is the storage chamber for the sample liquid to be tested, and the sample liquid to be tested is injected into it, and the fifth through hole 221 is connected to the external air source.

[0058] The first body 110 is rotated so that the plurality of protrusions 310 correspond to the positions of the plurality of first through holes 112 respectively.

[0059] The first body 110 is pressed downward toward the bottom surface of the blind hole 211, so that the protrusions 310 pierce the first sealing film 120, so that the sixth through holes 311 are connected to the first through holes 112 respectively, and the sealing pad 400 is deformed. The first body is rotated so that the first through holes 112 corresponding to the storage chamber of the sample liquid to be tested correspond to the positions of the seventh through holes 410. The external gas source is started to draw out the sample liquid to be tested and flow it into the third chamber to react with the preset protein molecule product; or the sample liquid to be tested flows back and forth between the third chamber and the liquid buffer 2122, so that the sample liquid to be tested fully reacts with the preset protein molecule product.

[0060] After the first preset time period, the external gas source is started to draw the sample liquid to be tested from the third chamber or the liquid buffer area 2122 and flow it into the waste liquid storage area 2123 of the second chamber.

[0061] The first body 110 is rotated clockwise to make the first through hole 112 corresponding to the first chamber 111 where the first cleaning liquid is located correspond to the position of the seventh through hole 410, and the external gas source is started to draw out the first cleaning liquid and flow it into the third chamber to clean the preset protein molecule product; or the first cleaning liquid is made to flow back and forth in the third chamber and the liquid buffer 2122 to make the cleaning operation more complete.

[0062] After the second preset time period, the external gas source is started to draw the first cleaning liquid out of the third chamber or the liquid buffer area 2122 and flow it into the waste liquid storage area 2123 .

[0063] The first body 110 is rotated clockwise so that the first through hole 112 corresponding to the first chamber 111 where the secondary antibody reagent is located corresponds to the position of the seventh through hole 410, and the external gas source is started to draw out the secondary antibody reagent and flow it into the third chamber to react with the preset protein molecule product; or the secondary antibody reagent is allowed to flow back and forth in the third chamber and the liquid buffer zone 2122 to allow the secondary antibody reagent to fully react with the preset protein molecule product.

[0064] After the third preset time period, the external gas source is started to draw the secondary antibody reagent out of the third chamber or the liquid buffer 2122 and flow it into the waste liquid storage area 2123 .

[0065] The first body 110 is rotated clockwise to make the first through hole 112 corresponding to the first chamber 111 where the second cleaning liquid is located correspond to the position of the seventh through hole 410, and the external gas source is started to draw out the second cleaning liquid and flow it into the third chamber to clean the preset protein molecule product; or the second cleaning liquid flows back and forth in the third chamber and the liquid buffer 2122 to make the cleaning operation more complete.

[0066] After the fourth preset time period, the external gas source is started to draw the second cleaning liquid out of the third chamber or the liquid buffer area 2122 and flow it into the waste liquid storage area 2123 .

[0067] The first body 110 is rotated clockwise to make the first through hole 112 corresponding to the first chamber 111 where the fluorescent reagent is located correspond to the position of the seventh through hole 410, and the external gas source is started to draw out the fluorescent reagent and flow it into the third chamber to react with the preset protein molecule product; or the fluorescent reagent is made to flow back and forth in the third chamber and the liquid buffer 2122 to allow the fluorescent reagent to fully react with the preset protein molecule product.

[0068] After the fifth preset time period, the external gas source is started to draw the fluorescent reagent out of the third chamber or the liquid buffer 2122 and flow it into the waste liquid storage area 2123 .

[0069] The first body 110 is rotated clockwise to make the first through hole 112 corresponding to the first chamber 111 where the third cleaning liquid is located correspond to the position of the seventh through hole 410, and the external gas source is started to draw out the third cleaning liquid and flow it into the third chamber to clean the preset protein molecule product; or the third cleaning liquid is made to flow back and forth in the third chamber and the liquid buffer 2122 to make the cleaning operation more complete.

[0070] After the sixth preset time period, the external gas source is started to draw the third cleaning liquid out of the third chamber or the liquid buffer area 2122 and flow it into the waste liquid storage area 2123 .

[0071] The protein coating position 231 is optically scanned and detected to complete the immunofluorescence biological detection of the sample to be detected.

[0072] Preferably, multiple test kits provided by the present invention can be integrated into an overall structure, so that multiple samples can be tested simultaneously, batch operations can be completed, and detection efficiency can be improved.

[0073] The test kit provided by the utility model makes the detection equipment miniaturized and easy to operate, and can achieve the technical effect of instant diagnosis by cooperating with the above-mentioned sample detection steps.

[0074] Although the embodiments of the present invention have been shown and described above, it can be understood that the above embodiments are illustrative and cannot be understood as limitations on the present invention. Ordinary technicians in this field can change, modify, replace and modify the above embodiments within the scope of the present invention. The scope of the present invention is defined by the claims and their equivalents.

Claims

1. A reagent storage assembly (100), characterized in that: include: A first body (110), wherein the first body (110) has a columnar structure; a plurality of first chambers (111) are arranged in the first body (110); a plurality of first through holes (112) and a plurality of second through holes (113) are respectively opened on the bottom surface and the top surface of the first body (110) in a number matching the number of the first chambers (111); the plurality of first through holes (112) and the plurality of second through holes (113) are respectively connected to the plurality of first chambers (111); the plurality of first through holes (112) are equidistant from the axis of the columnar structure; A second body (210), wherein the second body (210) has a three-dimensional structure, a blind hole (211) is formed on the top surface of the second body (210), the blind hole (211) matches the size of the first body (110), and a third through hole (214) is formed on the bottom surface of the blind hole (211); and the first through hole (112) can correspond to the position of the third through hole (214).

2. The reagent storage assembly (100) according to claim 1, characterized in that: Also includes: A first sealing film (120); the first sealing film (120) is sealingly connected to the bottom surface of the first body (110) to seal the first through holes (112); An ejector plate (300) has a size matching that of the blind hole (211), and has a plurality of protrusions (310) on its surface; the protrusions (310) match the shape and size of the second through hole (113); the plurality of protrusions (310) respectively correspond to the positions of the plurality of second through holes (113); the plurality of protrusions (310) respectively have a sixth through hole (311) penetrating the top of the protrusions and the bottom surface of the ejector plate (300), and the sixth through hole (311) can correspond to the position of the third through hole (214); the ejector plate (300) is placed at the bottom of the blind hole (211), and the first body (110) is placed on the ejector plate (300).

3. The reagent storage assembly (100) according to claim 1, characterized in that: It also comprises a second sealing film (130); the second sealing film (130) is sealingly connected to the top surface of the first body (110) to seal the plurality of second through holes (113).

4. The reagent storage assembly (100) according to claim 1, characterized in that: A first hole (140) is formed on the top surface of the first body (110), and the cross section of the first hole (140) is one of a polygonal shape, an elliptical shape or an irregular shape.

5. A kit, characterized in that include: The reagent storage assembly (100) as claimed in claim 2; the second body (210) is further provided with a first groove (212) on the top surface, and a second groove (213) on the bottom surface; the third through hole (214) connects the blind hole (211) with the second groove (213), and a fourth through hole (215) is provided between the first groove (212) and the second groove (213); A panel (220), the bottom surface of which is sealedly connected to the top surface of the second body (210), the bottom surface of the panel (220) and the first groove (212) forming a second chamber; a fifth through hole (221) is formed on the top surface of the panel (220), and the fifth through hole (221) is communicated with the second chamber; A bottom plate (230) is provided with a protein coating position (231) on its top surface, and a preset protein molecule product is fixed on the protein coating position (231); the top surface of the bottom plate (230) is sealedly connected to the bottom surface of the second body (210), and the top surface of the bottom plate (230) and the second groove (213) form a third chamber, and the protein coating position (231) is located in the third chamber.

6. The kit according to claim 5, characterized in that The first groove (212) comprises a liquid flow area (2121) and a liquid buffer area (2122), and the cross-sectional area of ​​the liquid flow area (2121) is smaller than the cross-sectional area of ​​the liquid buffer area (2122).

7. The kit according to claim 6, characterized in that The first groove (212) also includes a waste liquid storage area (2123), and the distance between the bottom of the waste liquid storage area (2123) and the top surface of the second body (210) is greater than the distance between the bottom of the liquid flow area (2121) and the liquid buffer area (2122) and the top surface of the second body (210).

8. The kit according to claim 7, characterized in that Also includes: A sealing gasket (400); the sealing gasket (400) is made of an elastic material; the sealing gasket (400) has a seventh through hole (410) penetrating the top and bottom surfaces thereof, the seventh through hole (410) corresponding to the position of the third through hole (214); the sealing gasket (400) is located between the ejector plate (300) and the bottom surface of the blind hole (211), and is fixedly connected to the bottom surface of the blind hole (211).

9. The kit according to claim 8, characterized in that At least two protrusions (216) are arranged on the side wall of the blind hole (211); the edge of the ejector plate (300) is provided with a first notch (320) corresponding in number to the protrusions (216) and matching in shape and position; the bottom surface of the first body (110) has a circular flange (114); the edge of the flange (114) is provided with a second notch (115) corresponding in number to the protrusions (216) and matching in shape and position; when the first body (110) is pressed downward to cause the sealing gasket (400) to deform, the first body (110) is rotated along the axis of the first body (110), and the upper surface of the flange (114) abuts against the lower surface of the protrusion (216).

10. The kit according to claim 8, characterized in that An eighth through hole is opened in the middle of the sealing pad (400); and a ninth through hole corresponding to the position of the eighth through hole is opened in the middle of the ejector plate (300).

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