Synchronous biological strain incubator for reverse osmosis strong brine discharge treatment

Abstract

The utility model relates to the technical field of reverse osmosis strong brine biological treatment, in particular to a synchronous biological strain incubator for reverse osmosis strong brine discharge treatment, which is characterized by comprising an intelligent control system, an expanding culture tank system, a feeding system, a circulating water system, an online monitoring system, an aeration system and a pipeline arrangement system. According to the technical scheme, culture parameters such as PH, dissolved oxygen and temperature in the tank can be dynamically monitored and adjusted, and it is ensured that halotolerant bacteria can be rapidly and efficiently bred after inoculation. And on the other hand, the seed tank system can realize refrigerated preservation of strains, and is combined with the culture tank to integrate the propagation process, so that manpower and material resources are saved.

Description

Technical Field

[0001] The utility model relates to the technical field of reverse osmosis concentrated brine biological treatment, in particular to an automated online culture device for aerobic nitrifying bacteria, heterotrophic nitrification-aerobic denitrifying bacteria and anaerobic denitrifying bacteria. Background Art

[0002] Reverse osmosis refers to the process in which two solutions of different concentrations have equal static pressure on both sides of a membrane, and the resulting osmotic pressure difference causes the low-concentration solution to flow through the membrane to the high-concentration solution. The characteristics of the reverse osmosis membrane are used to separate the treated water into demineralized water and concentrated brine. Pollutants such as ammonia nitrogen, nitrate nitrogen and total nitrogen in the concentrated brine are concentrated, so they need to be treated before discharge.

[0003] The reverse osmosis brine treatment process mainly includes physical and chemical treatment, biological treatment and advanced oxidation process. Among them, the biological treatment method based on microbial action is essentially to use wastewater containing pollutants as a culture medium, continuously cultivate mixed microbial populations under appropriate conditions, and transform pollutants into substances that are harmless to the environment through the action of microorganisms.

[0004] Microorganisms are involved in quite a lot of mechanisms in wastewater treatment, including the staged processes of hydrolysis acidification, acetogenesis and methanogenesis in anaerobic biological treatment; in aerobic biological treatment, aerobic microorganisms decompose organic matter through dissimilatory metabolism, assimilation and self-oxidation of cellular substances under the action of relevant enzymes. Among them, the use of biological denitrification to remove total nitrogen from reverse osmosis brine involves traditional aerobic nitrification and anaerobic denitrification as well as the new heterotrophic nitrification-aerobic denitrification. Nitrification refers to the process in which ammonia removed from amino acids is converted into nitric acid under aerobic conditions by nitrite bacteria and nitrate bacteria; denitrification is the process in which microorganisms reduce nitrates and nitrites to gaseous nitrogen compounds and nitrogen gas under anaerobic conditions, and is the main biological process in which active nitrogen returns to the atmosphere in the form of nitrogen gas. The denitrification of heterotrophic nitrification-aerobic denitrifiers mainly includes assimilation and dissimilation processes.

[0005] In each treatment process, environmental factors such as pH, dissolved oxygen, and temperature play a decisive role in the growth and reproduction of microorganisms and their functional efficiency. Therefore, it is easy for the set environmental parameters to be impacted during the microbial culture process due to different stages of the reaction process. Although the existing biological expansion devices try their best to provide a suitable environment for the growth, development and reproduction of microorganisms, they cannot adjust the changes in the expansion tank in real time, thereby reducing the expansion efficiency. On the other hand, the automation and integration of the expansion equipment are not strong, and the need for professional and technical workers to operate limits the use of the equipment. Finally, the expansion process needs to be able to cultivate both aerobic nitrifying bacteria and heterotrophic nitrification-aerobic denitrifying bacteria, as well as anaerobic denitrifying bacteria, that is, it needs to have an aerobic and anaerobic alternating culture mode to increase the application range of the equipment. Summary of the invention

[0006] The utility model has made a corresponding technical solution to the above-mentioned problems, namely, through the intelligent control system, the environmental parameters are dynamically monitored and adjusted so that the microorganisms are always in a suitable environment for growth and reproduction, and through the pipeline layout and the control of the opening and closing of the electromagnetic valve, the device is automatically operated according to the specified program, thus overcoming the problem of cumbersome manual operation and improving work efficiency. Moreover, the seed tank system can realize the refrigerated storage of bacterial strains, and combined with the culture tank, the expansion and cultivation process is integrated, which saves manpower and material resources and improves production efficiency. Finally, sterile air is introduced into the culture tank through an air compressor and a filter to provide an aerobic environment for nitrifying bacteria and heterotrophic nitrification-aerobic denitrifying bacteria; nitrogen is introduced into the culture tank through a nitrogen generator to provide an anaerobic environment for denitrifying bacteria, so that the types of microorganisms cultivated by the equipment are more comprehensive.

[0007] In order to solve the above technical problems, the utility model provides a synchronous biological strain culture device for reverse osmosis concentrated brine discharge treatment, characterized in that it includes an intelligent control system, an expansion tank system, a feeding system, a circulating water system, an online monitoring system, an aeration system and a pipeline layout system, the expansion tank system includes two expansion tank bodies, the bottom of the expansion tank body is provided with a bracket for suspended support, one of the expansion tank bodies is a seed tank for preserving strains, and the other is a culture tank for cultivating strains, the discharge port at the bottom of the culture tank is connected to the seed tank through a discharge pipe, a seed tank transfer valve is installed on the discharge pipe, the discharge pipe is also connected to a bacterial liquid discharge pipe, a bacterial liquid discharge valve is installed on the bacterial liquid discharge pipe, and the pipeline layout system is composed of a compressed air pipeline, a nitrogen pipeline, a steam pipeline, a circulating water pipeline, a feeding pipeline and The culture tank body is composed of a drainage pipeline, a jacket is arranged on the lower outer wall of the expansion tank body, the inlet and outlet of the jacket are connected to the circulating water system through a circulating water pipeline, a feeding port is arranged on the top of the culture tank, a seed tank flame inoculation port is arranged on the top of the seed tank, nutrient agent and sewage inlets are arranged on the top of the culture tank and the seed tank, the nutrient agent and sewage inlets are connected to the feeding system through a feeding pipeline, the feeding pipeline is also connected to the drainage tank through a drainage pipeline, the upper part of the expansion tank body is connected to a compressed air pipeline and a nitrogen pipeline, the compressed air pipeline and the circulating water pipeline are both connected to a steam pipeline, the expansion tank body is also connected to an online monitoring system and an aeration system, and the compressed air pipeline, nitrogen pipeline, steam pipeline, circulating water pipeline, feed pipeline and drainage pipeline are all provided with solenoid valves or pneumatic valves controlled by an intelligent control system.

[0008] Furthermore, a pressure gauge, a safety valve and a low-speed motor are installed on the top of the expansion tank body, and the output shaft of the low-speed motor extends into the expansion tank body and is connected to the stirring mechanism.

[0009] Furthermore: the steam pipeline connected to the circulating water pipeline is a second steam pipeline, and the second steam pipeline is connected to one end of the circulating water pipeline connected to the jacket inlet.

[0010] Furthermore: the circulating water system includes a self-priming pump, a hot water tank and a tap water tank. The self-priming pump is installed on the circulating water pipeline, and the circulating water pipeline is also connected to the hot water tank and the tap water tank. A chiller is also arranged on the circulating water pipeline connected to the outer jacket of the seed tank. The self-priming pump and the chiller are both controlled to open and close by the intelligent control system.

[0011] Furthermore: a nitrogen inlet is arranged on the side wall of the expansion tank body, the nitrogen inlet is connected to a nitrogen buffer tank through a nitrogen pipeline, and the nitrogen buffer tank is connected to a nitrogen generator.

[0012] Furthermore: the aeration system includes an air compressor, an air filter and an aeration plate, the aeration plate is arranged in the lower end of the expansion tank body, the aeration plate is connected to the compressed air pipeline through an aeration pipeline, the compressed air pipeline is connected to the air storage tank through the air filter, the air storage tank is connected to the air compressor, and the steam pipeline connected to the compressed air pipeline is the first steam pipeline.

[0013] Furthermore: a sterile air inlet is arranged on the upper side wall of the culture expansion tank, and the sterile air inlet is connected to a compressed air pipeline.

[0014] Furthermore: the online monitoring system includes a liquid level meter, an air flow meter, a pH electrode, a dissolved oxygen electrode and a temperature electrode. The liquid level meter is arranged on the top of the expansion tank body, the air flow meter is arranged on the compressed air pipeline, the pH electrode, the dissolved oxygen electrode and the temperature electrode are arranged on the side wall of the lower part of the expansion tank body, and the liquid level meter, air flow meter, pH electrode, dissolved oxygen electrode and temperature electrode are all electrically connected to the intelligent control system.

[0015] Furthermore: an exhaust port is arranged on the side wall of the upper part of the expansion tank body, a regulator and defoaming agent inlet is arranged on the side wall of the expansion tank body above the jacket, a sampling port is arranged on the side wall of the lower part of the expansion tank body, and the exhaust port, the regulator and defoaming agent inlet and the sampling port are all provided with valves controlled by the intelligent control system.

[0016] Furthermore: a viewing mirror for observing the internal situation is also arranged on the side wall of the expansion tank.

[0017] After adopting the above structure, the technical solution of the utility model can dynamically monitor and adjust the culture parameters such as pH, dissolved oxygen and temperature in the tank to ensure that the salt-tolerant bacteria can reproduce quickly and efficiently after inoculation. The intelligent control system simplifies manual operation, and non-technical personnel can also complete the entire expansion process. Moreover, the utility model can realize the efficient cultivation of nitrification, heterotrophic nitrification-aerobic denitrification and denitrifying bacteria, greatly improving the practicality and convenience of the equipment. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] The utility model is further described in detail below in conjunction with the accompanying drawings and specific implementation methods.

[0019] Figure 1 It is a structural schematic diagram of the utility model. DETAILED DESCRIPTION

[0020] like Figure 1A synchronous biological strain culture device for reverse osmosis concentrated brine discharge treatment is shown, comprising an intelligent control system, an expansion tank system, a feed system, a circulating water system, an online monitoring system, an aeration system and a pipeline layout system, wherein the expansion tank system comprises two expansion tank bodies, and a bracket 7 for suspended support is provided at the bottom of the expansion tank body, wherein one of the expansion tank bodies is a seed tank 32 for strain preservation, and the other is a culture tank 1 for strain cultivation, and the discharge port at the bottom of the culture tank is connected to the seed tank through a discharge pipe, and a seed tank transfer valve 29 is installed on the discharge pipe, and the discharge pipe is also connected to a bacterial liquid discharge pipe, and a bacterial liquid discharge valve 28 is installed on the bacterial liquid discharge pipe, and the pipeline layout system is composed of a compressed air pipeline, a nitrogen pipeline, a steam pipeline, a circulating water pipeline, a feed pipeline and a sewage pipeline, and the A jacket 8 is provided on the lower outer wall of the expansion tank body, and the inlet and outlet of the jacket are connected to the circulating water system through a circulating water pipeline. A feeding port 2 is provided on the top of the culture tank, and a seed tank flame inoculation port 14 is provided on the top of the seed tank. Nutrient and sewage inlets 22 are provided on the tops of the culture tank and the seed tank, and the nutrient and sewage inlets are connected to the feeding system through a feeding pipeline, and the feeding pipeline is also connected to the sewage tank through a sewage pipeline. The upper part of the expansion tank body is connected to a compressed air pipeline and a nitrogen pipeline, and the compressed air pipeline and the circulating water pipeline are both connected to a steam pipeline. The expansion tank body is also connected to an online monitoring system and an aeration system, and the compressed air pipeline, nitrogen pipeline, steam pipeline, circulating water pipeline, feed pipeline and sewage pipeline are all provided with solenoid valves or pneumatic valves controlled by an intelligent control system. The nutrient inside the tank of the utility model is sterilized by high-pressure steam, and the strain transfer in the tank is achieved by pressure difference. The pipeline is also sterilized by high-pressure steam to avoid contamination by miscellaneous bacteria. In addition, this solution can dynamically monitor and adjust the culture parameters such as pH, dissolved oxygen and temperature in the tank to ensure that the salt-tolerant bacteria can reproduce quickly and efficiently after inoculation. The manual operation is simplified by the intelligent control system, and non-technical personnel can also complete the entire expansion process. Moreover, the utility model can realize the efficient cultivation of nitrification, heterotrophic nitrification-aerobic denitrification and denitrifying bacteria, greatly improving the practicality and convenience of the equipment.

[0021] like Figure 1 The top of the expansion tank is equipped with a pressure gauge 3, a safety valve 5 and a low-speed motor 4, the output shaft of which extends into the expansion tank and is connected to the stirring mechanism. The utility model adopts the above structure to ensure the safety of operation, and the low-speed stirring helps to make the bacterial liquid uniform, increase the contact with air, improve the dissolved oxygen concentration, and reduce the damage of the shear force of stirring to the bacterial strain.

[0022] like Figure 1The steam pipeline connected to the circulating water pipeline is a second steam pipeline 25, and the second steam pipeline is connected to one end of the circulating water pipeline connected to the jacket inlet.

[0023] like Figure 1 The circulating water system shown includes a self-priming pump, a hot water tank and a tap water tank. The self-priming pump is installed on the circulating water pipeline, which is also connected to the hot water tank and the tap water tank. A chiller is also provided on the circulating water pipeline connected to the outer jacket of the seed tank. The self-priming pump and the chiller are both controlled to open and close by the intelligent control system. The hot water, tap water and cooling water in the jacket can circulate to keep the temperature in the tank constant. The chiller is connected to the inlet and outlet water pipelines of the seed tank jacket, which can refrigerate and preserve the strains to prevent strain aging and reduce the treatment effect.

[0024] like Figure 1 A nitrogen inlet 23 is provided on the side wall of the expansion tank body, and the nitrogen inlet is connected to the nitrogen buffer tank 18 through a nitrogen pipeline, and the nitrogen buffer tank is connected to the nitrogen generator 17.

[0025] like Figure 1 The aeration system shown includes an air compressor 15, an air filter 19 and an aeration plate 30. The aeration plate is arranged in the lower end of the expansion tank body. The aeration plate is connected to the compressed air pipeline through the aeration pipeline 26. The compressed air pipeline is connected to the air storage tank 16 through the air filter 19. The air storage tank is connected to the air compressor 15. The steam pipeline connected to the compressed air pipeline is the first steam pipeline 20. The aeration plate is located at the bottom of the tank body and is connected to the compressed air pipeline. The ventilation volume is controlled by the air flow meter to ensure the dissolved oxygen content of the culture in the tank.

[0026] like Figure 1 A sterile air inlet 21 is provided on the upper side wall of the expansion culture tank, and the sterile air inlet is connected to the compressed air pipeline.

[0027] like Figure 1The online monitoring system shown includes a liquid level meter, an air flow meter, a pH electrode 10, a dissolved oxygen electrode 11 and a temperature electrode 12. The liquid level meter is arranged on the top of the expansion tank body, the air flow meter is arranged on the compressed air pipeline, the pH electrode, the dissolved oxygen electrode and the temperature electrode are arranged on the side wall of the lower part of the expansion tank body, and the liquid level meter, the air flow meter, the pH electrode, the dissolved oxygen electrode and the temperature electrode are all electrically connected to the intelligent control system. The pH electrode, the dissolved oxygen electrode and the temperature electrode can monitor the changes in the tank in real time, and if the pH is lower than or higher than the set parameter, the intelligent control system opens the pneumatic valve 9, and the peristaltic pump pumps the pH regulator into the tank; if the dissolved oxygen is lower than or higher than the set parameter, the dissolved oxygen is changed by adjusting the air flow; if the temperature is lower than or higher than the set parameter, the hot water tank is started to heat or the jacket is cooled by low-inlet and high-outlet tap water.

[0028] like Figure 1 An exhaust port 31 is arranged on the side wall of the upper part of the expansion tank body shown in the figure, a regulator and defoaming agent inlet 9 is arranged on the side wall of the expansion tank body above the jacket, and a sampling port 13 is arranged on the side wall of the lower part of the expansion tank body. The exhaust port, the regulator and defoaming agent inlet and the sampling port are all provided with valves controlled by the intelligent control system; a sight glass 6 for observing the internal situation is also arranged on the side wall of the expansion tank body.

[0029] The above are only preferred implementations of the utility model. The protection scope of the utility model is not limited to the above embodiments. All technical solutions under the idea of ​​the utility model belong to the protection scope of the utility model. It should be pointed out that for ordinary technicians in this technical field, some improvements and modifications without departing from the principle of the utility model should be regarded as the protection scope of the utility model.

Claims

1. A synchronous biological strain culture device for reverse osmosis concentrated brine effluent treatment, characterized in that: The invention comprises an intelligent control system, a culture expansion tank system, a feeding system, a circulating water system, an online monitoring system, an aeration system and a pipeline layout system. The culture expansion tank system comprises two culture expansion tank bodies. A bracket (7) for suspended support is arranged at the bottom of the culture expansion tank body. One of the culture expansion tank bodies is a seed tank (32) for preserving bacterial strains, and the other is a culture tank (1) for cultivating bacterial strains. The discharge port at the bottom of the culture tank is connected with the seed tank through a discharge pipe. A seed tank transfer valve (29) is installed on the discharge pipe. The discharge pipe is also connected with a bacterial liquid discharge pipe. A bacterial liquid discharge valve (28) is installed on the bacterial liquid discharge pipe. The pipeline layout system is composed of a compressed air pipeline, a nitrogen pipeline, a steam pipeline, a circulating water pipeline, a feeding pipeline and a sewage pipeline. The lower outer wall of the culture expansion tank body is provided with a The jacket (8) is connected to the circulating water system through a circulating water pipeline. The top of the culture tank is provided with a feed port (2). The top of the seed tank is provided with a seed tank flame inoculation port (14). The tops of the culture tank and the seed tank are provided with nutrient agent and sewage inlets (22). The nutrient agent and sewage inlets are connected to the feed system through a feed pipeline. The feed pipeline is also connected to a sewage tank through a sewage pipeline. The upper part of the expansion tank body is connected to a compressed air pipeline and a nitrogen pipeline. The compressed air pipeline and the circulating water pipeline are both connected to a steam pipeline. The expansion tank body is also connected to an online monitoring system and an aeration system. The compressed air pipeline, nitrogen pipeline, steam pipeline, circulating water pipeline, feed pipeline and sewage pipeline are all provided with solenoid valves or pneumatic valves controlled by an intelligent control system.

2. A synchronous biological strain culture device for reverse osmosis concentrated brine effluent treatment according to claim 1, characterized in that: A pressure gauge (3), a safety valve (5) and a low-speed motor (4) are installed on the top of the cultivation expansion tank. The output shaft of the low-speed motor extends into the cultivation expansion tank and is connected to the stirring mechanism.

3. A synchronous biological strain culture device for reverse osmosis concentrated brine effluent treatment according to claim 1, characterized in that: The steam pipeline connected to the circulating water pipeline is a second steam pipeline (25), and the second steam pipeline is connected to one end of the circulating water pipeline connected to the jacket inlet.

4. A synchronous biological strain culture device for reverse osmosis concentrated brine effluent treatment according to any one of claims 1 or 3, characterized in that: The circulating water system includes a self-priming pump, a hot water tank and a tap water tank. The self-priming pump is installed on a circulating water pipeline, which is also connected to the hot water tank and the tap water tank. A chiller is also provided on the circulating water pipeline connected to the outer jacket of the seed tank. The self-priming pump and the chiller are both controlled to open and close by the intelligent control system.

5. A synchronous biological strain culture device for reverse osmosis concentrated brine effluent treatment according to claim 1, characterized in that: A nitrogen inlet (23) is provided on the side wall of the expansion tank body, and the nitrogen inlet is connected to a nitrogen buffer tank (18) through a nitrogen pipeline, and the nitrogen buffer tank is connected to a nitrogen generator (17).

6. A synchronous biological strain culture device for reverse osmosis concentrated brine effluent treatment according to claim 1, characterized in that: The aeration system comprises an air compressor (15), an air filter (19) and an aeration plate (30). The aeration plate is arranged in the lower end of the expansion tank body. The aeration plate is connected to a compressed air pipeline via an aeration pipeline (26). The compressed air pipeline is connected to an air storage tank (16) via an air filter (19). The air storage tank is connected to the air compressor (15). The steam pipeline connected to the compressed air pipeline is a first steam pipeline (20).

7. A synchronous biological strain culture device for reverse osmosis concentrated brine effluent treatment according to any one of claims 1 or 6, characterized in that: A sterile air inlet (21) is arranged on the upper side wall of the culture expansion tank, and the sterile air inlet is connected to a compressed air pipeline.

8. The synchronous biological strain culture device for reverse osmosis concentrated brine effluent treatment according to claim 1, characterized in that: The online monitoring system comprises a liquid level meter, an air flow meter, a pH electrode (10), a dissolved oxygen electrode (11) and a temperature electrode (12); the liquid level meter is arranged on the top of the culture tank body; the air flow meter is arranged on the compressed air pipeline; the pH electrode, the dissolved oxygen electrode and the temperature electrode are arranged on the side wall of the lower part of the culture tank body; the liquid level meter, the air flow meter, the pH electrode, the dissolved oxygen electrode and the temperature electrode are all electrically connected to the intelligent control system.

9. A synchronous biological strain culture device for reverse osmosis concentrated brine effluent treatment according to claim 1, characterized in that: An exhaust port (31) is arranged on the side wall of the upper part of the expansion tank body, a regulator and defoamer inlet (9) is arranged on the side wall of the expansion tank body above the jacket, and a sampling port (13) is arranged on the side wall of the lower part of the expansion tank body. The exhaust port, the regulator and defoamer inlet and the sampling port are all provided with valves controlled by an intelligent control system.

10. The synchronous biological strain culture device for reverse osmosis concentrated brine effluent treatment according to claim 1, characterized in that: A viewing mirror (6) for observing the internal conditions is also arranged on the side wall of the expansion tank.

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