High-speed fluorescence detection device

Abstract

The utility model relates to a high-speed fluorescence detection device, which comprises a fluorescence detection module and a driving mechanism, and the driving mechanism is used for driving the fluorescence detection module to move so as to enable the fluorescence detection module to respectively detect a plurality of samples at different positions. The high-speed fluorescence detection device is high in detection flux and low in cost.

Description

Technical Field

[0001] The utility model relates to the technical field of in vitro diagnosis, in particular to a high-speed fluorescence detection device. Background Art

[0002] Liquid culture of Mycobacterium tuberculosis has become the gold standard for diagnosing tuberculosis due to its advantages such as good specificity. After liquid culture, the fluorescence intensity detection technology is used for detection. This detection technology achieves the purpose of detection by monitoring the changes in fluorescence intensity caused by the consumption of oxygen by the growth of mycobacteria.

[0003] Due to its high sensitivity and high specificity, fluorescence detection is also widely used in other in vitro diagnostic fields besides Mycobacterium tuberculosis detection. The sample size of fluorescence detection devices is generally large, and existing fluorescence detection devices often have multiple fluorescence detection modules to meet the needs of high-throughput detection, that is, one fluorescence detection module corresponds to one sample. Since the equipment is generally small in size and each fluorescence detection module is relatively large, this design can only meet the needs of a certain number of samples. It is difficult for the detection device to meet the requirements for a large number of samples. At the same time, the large number of fluorescence detection devices leads to relatively high costs.

[0004] In addition, the prior art cannot detect whether there is a sample at the location where the sample is placed, thus resulting in invalid detection. Utility Model Content

[0005] The purpose of the utility model is to provide a high-speed fluorescence detection device to at least solve one of the above technical problems.

[0006] To achieve the above object, the utility model provides a high-speed fluorescence detection device, including a fluorescence detection module and a driving mechanism, wherein the driving mechanism is used to drive the fluorescence detection module to move so that the fluorescence detection module can detect multiple samples located at different positions respectively.

[0007] Optionally, the driving mechanism is used to drive the fluorescence detection module to move back and forth along a first direction and a second direction, and the first direction and the second direction are perpendicular.

[0008] Optionally, the high-speed fluorescence detection device further comprises an in-situ detection mechanism connected to the driving mechanism, wherein the in-situ detection mechanism is used to detect whether there are samples at a plurality of preset positions, and the preset positions are used to place samples.

[0009] Optionally, the in-situ detection mechanism includes one or more in-situ detection modules.

[0010] Optionally, when there are multiple in-place detection modules, the multiple in-place detection modules are arranged in sequence along the second direction, and the driving mechanism is used to drive the multiple in-place detection modules to move along the first direction.

[0011] Optionally, the high-speed fluorescence detection device further comprises a carrying portion, wherein the carrying portion is provided with a plurality of carrying positions, and the carrying positions are used to carry samples; optionally, the plurality of carrying positions are arranged in an array along the first direction and the second direction.

[0012] Optionally, the driving mechanism includes a first driving assembly and a second driving assembly, and the first driving assembly is connected to the second driving assembly to drive the second driving assembly to move along the first direction;

[0013] The second driving component is connected to the fluorescence detection module to drive the fluorescence detection module to move along a second direction; and / or

[0014] When there are multiple in-place detection modules, the first driving component is connected to the in-place detection mechanism to drive the in-place detection module to move along the first direction; when there is only one in-place detection module, the second driving component is connected to the in-place detection module to drive the in-place detection module to move along the second direction.

[0015] Optionally, the high-speed fluorescence detection device further includes a bearing seat; and the first driving assembly includes:

[0016] A first driving member connected to the bearing seat;

[0017] A first driving pulley and a first driven pulley are spaced apart in a first direction and rotatably connected to the bearing seat, and the first driving pulley is connected to the first driving member;

[0018] A first belt is sleeved outside the first driving pulley and the first driven pulley, and the second driving assembly is connected to the first belt;

[0019] And / or, the second driving assembly includes:

[0020] A mounting portion connected to the first driving assembly;

[0021] A second driving member connected to the mounting portion;

[0022] A second driving pulley and a second driven pulley are spaced apart in a second direction and rotatably connected to the mounting portion, and the second driving pulley is connected to the second driving member;

[0023] A second belt is sleeved outside the second driving pulley and the second driven pulley, and the fluorescence detection module is connected to the second belt;

[0024] The in-place detection module is connected to the first belt or the second belt.

[0025] Optionally, the second driving assembly further includes at least two tensioning wheels, which are arranged between the second driving pulley and the second driven pulley and are used to tension the second belt.

[0026] Optionally, the driving mechanism further comprises a first guide assembly, wherein the first guide assembly is used to guide the second driving assembly; and / or

[0027] The driving mechanism further comprises a second guiding component, and the second guiding component is used to guide the fluorescence detection module;

[0028] When there are multiple in-situ detection modules, the first guide component can guide the in-situ detection mechanism; when there is only one in-situ detection module, the second guide component can guide the in-situ detection mechanism.

[0029] Optionally, the first guide assembly includes a first guide rail and a first guide block, the first guide rail extends along a first direction, the first guide block is slidably disposed on the first guide rail, and the second drive assembly is connected to the first guide block; and / or

[0030] The second guide assembly includes a second guide rail and a second guide block, the second guide rail extends along a second direction, the second guide block is slidably disposed on the second guide rail, and the fluorescence detection module is connected to the second guide block;

[0031] When there are multiple in-place detection modules, the first guide block is connected to the in-place detection mechanism; when there is only one in-place detection module, the second guide block is connected to the in-place detection mechanism.

[0032] Optionally, the in-situ detection mechanism further includes a mounting plate, wherein the mounting plate is connected to the driving mechanism, and the in-situ detection module is connected to the mounting plate.

[0033] Optionally, the first direction and the second direction are located in a horizontal plane.

[0034] As can be seen from the above, the technical solution provided by the utility model, the driving mechanism can move the fluorescence detection module to different positions, so as to detect samples at different positions, that is, one fluorescence detection module can correspond to multiple samples, the high-speed fluorescence detection device has a large detection flux, low equipment cost, and small equipment size, which solves the problem in the prior art that one fluorescence detection module needs to correspond to one sample, resulting in low detection flux and high equipment cost.

[0035] The high-speed fluorescence detection device also includes an in-situ detection mechanism connected to the driving mechanism, and the in-situ detection mechanism is used to detect whether there are samples at multiple preset positions, and the preset positions are used to place samples. In this embodiment, the preset position refers to the load-bearing position, that is, the in-situ detection mechanism is used to detect whether there are samples on the load-bearing position. If there are samples on the load-bearing position, the fluorescence detection module detects the sample. If there are no samples on the load-bearing position, the fluorescence detection module does not need to detect the sample at the load-bearing position. The in-situ detection mechanism can pre-identify whether there are samples. The pre-identification function of the in-situ detection mechanism is integrated with the fluorescence detection function. As a new application method, it can effectively improve the detection efficiency and subsequent data processing efficiency, especially in high-throughput detection, it can effectively improve the subsequent data processing efficiency. It only takes about 10 minutes to complete the 400 hole position test using the high-speed fluorescence detection device provided by the utility model. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] Figure 1a It is a structural schematic diagram of a high-speed fluorescence detection device provided by an embodiment of the utility model;

[0037] Figure 1b It is a schematic diagram of the structure of the high-speed fluorescence detection device provided by the embodiment of the utility model with part of the side wall removed;

[0038] Figure 1c yes Figure 1b A partial enlarged view of point C in the middle;

[0039] Figure 1d It is a structural schematic diagram of another perspective of the high-speed fluorescence detection device provided by an embodiment of the utility model with part of the side wall removed;

[0040] Figure 2a This is a partial structural schematic diagram of a high-speed fluorescence detection device provided by an embodiment of the utility model;

[0041] Figure 2b yes Figure 2a A partial enlarged view of the middle A;

[0042] Figure 2c yes Figure 2a A partial enlarged view of point B in the middle;

[0043] Figure 3a This is a second schematic diagram of a part of the structure of the high-speed fluorescence detection device provided by an embodiment of the utility model;

[0044] Figure 3b yes Figure 3a A partial enlarged view of point D in the middle;

[0045] Figure 4 It is a flow chart of the fluorescence detection method provided by the embodiment of the utility model.

[0046] In the figure:

[0047] 11. Fluorescence detection module;

[0048] 12. Driving mechanism; 121. First driving assembly; 1211. First driving member; 1212. First driving pulley; 1213. First driven pulley; 1214. First belt; 122. Second driving assembly; 1221. Mounting portion; 1222. Second driving pulley; 1223. Second driven pulley; 1224. Second belt; 1225. Tensioning wheel; 123. First guide assembly; 1231. First guide rail; 1232. First guide block; 124. Second guide assembly; 1241. Second guide rail; 1242. Second guide block;

[0049] 13. In-situ detection mechanism; 131. In-situ detection module; 132. Mounting plate;

[0050] 15. housing; 153. bearing seat; 154. bearing portion; 1541. bearing position; 155. side wall;

[0051] 100. Culture tube. DETAILED DESCRIPTION

[0052] The technical solution of the utility model is further described below in conjunction with the accompanying drawings and through specific implementation methods. It is understood that the specific embodiments described herein are only used to explain the utility model, rather than to limit the utility model. It is also necessary to explain that, for the convenience of description, only the parts related to the utility model are shown in the accompanying drawings, rather than all.

[0053] Some directional words are defined in the present utility model. Unless otherwise specified, the directional words used, such as "upper", "lower", "left", "right", "inside" and "outside", are used for ease of understanding and therefore do not constitute limitations on the protection scope of the present utility model.

[0054] In the present utility model, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may include that the first and second features are in direct contact, or may include that the first and second features are not in direct contact but are in contact through another feature between them. Moreover, a first feature being "above", "above" and "above" a second feature includes that the first feature is directly above and obliquely above the second feature, or simply indicates that the first feature is higher in level than the second feature. A first feature being "below", "below" and "below" a second feature includes that the first feature is directly below and obliquely below the second feature, or simply indicates that the first feature is lower in level than the second feature.

[0055] In the description of the present invention, unless otherwise clearly specified and limited, the terms "connected", "connected", and "fixed" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0056] This embodiment provides a high-speed fluorescence detection device, which is used in PCR detection, but is not limited thereto, to increase detection throughput and reduce equipment costs.

[0057] like Figure 1a-Figure 3a As shown, the high-speed fluorescence detection device includes a fluorescence detection module 11 and a driving mechanism 12. The driving mechanism 12 is used to drive the fluorescence detection module 11 to move so that the fluorescence detection module 11 can detect multiple samples located at different positions. The driving mechanism 12 can move the fluorescence detection module 11 to different positions, so as to detect samples at different positions, that is, one fluorescence detection module 11 can correspond to multiple samples. The high-speed fluorescence detection device has a large detection flux, low equipment cost, and small equipment volume, which solves the problem in the prior art that one fluorescence detection module 11 needs to correspond to one sample, resulting in a small detection flux and high equipment cost.

[0058] Exemplarily, the fluorescence detection module 11 may include an excitation light source and a photoelectric sensor. The excitation light source emits light to excite the fluorescent substance at the bottom of the culture tube 100, and the fluorescent substance generates fluorescence and is received by the photoelectric sensor. Finally, the control mechanism realizes the analysis of the fluorescence signal. The fluorescence detection module 11 may be an existing module that can detect fluorescence signals, and will not be described in detail here.

[0059] Optionally, the control mechanism can be a centralized or distributed controller. For example, the control mechanism can be a single single-chip microcomputer or a plurality of distributed single-chip microcomputers. The control program can be run in the single-chip microcomputer to control the above-mentioned components to realize their functions.

[0060] like Figure 1b and Figure 1d As shown, illustratively, the fluorescence detection module 11 provided in this embodiment is used to detect the sample located above it. In this embodiment, the sample is placed in a culture tube 100, and the lower end of the culture tube 100 is light-transmissive so that the fluorescence detection module 11 can detect the sample through the culture tube 100.

[0061] like Figure 1b and Figure 1cAs shown, illustratively, the high-speed fluorescence detection device may further include a carrying portion 154, the carrying portion 154 is provided with a plurality of carrying positions 1541, the carrying positions 1541 are used to carry samples, specifically, the culture tube 100 is placed in the carrying position 1541, and the sample is placed in the carrying position 1541 through the culture tube 100. In this embodiment, 400 carrying positions 1541 may be provided, of course, the number of the carrying positions 1541 is not limited thereto, and the high-speed fluorescence detection device of this embodiment may meet the goal of high-throughput detection.

[0062] Optionally, a plurality of bearing positions 1541 are arranged in an array along the first direction X and the second direction Y to improve the detection flux. Exemplarily, the first direction X and the second direction Y are in a horizontal plane, that is, the first direction X, the second direction Y and the vertical direction Z are perpendicular to each other. In other optional embodiments, the first direction X and the second direction Y may also be in other planes. For example, the first direction X and the second direction Y may also be on an inclined plane at an acute angle to the horizontal plane.

[0063] Furthermore, the driving mechanism 12 is used to drive the fluorescence detection module 11 to reciprocate along the first direction X and the second direction Y. Exemplarily, the driving mechanism 12 drives the fluorescence detection module 11 to move along the second direction Y to respectively detect a row of samples placed along the second direction Y, and then the driving mechanism 12 drives the fluorescence detection module 11 to move along the first direction X to move the fluorescence detection module 11 to the next row of samples, and the above actions are repeated until the fluorescence detection module 11 has detected all the samples.

[0064] Optionally, the high-speed fluorescence detection device may further include a bearing seat 153, and the driving mechanism 12 is connected to the bearing seat 153. Exemplarily, the bearing seat 153 may be located below the driving mechanism 12. Optionally, the high-speed fluorescence detection device may include a housing 15, and the housing 15 may include the above-mentioned bearing seat 153 and a bearing portion 154 located above the bearing seat 153, and the bearing seat 153 and the bearing portion 154 may be connected through a side wall 155.

[0065] like Figure 2a-Figure 3b As shown, the driving mechanism 12 may include a first driving component 121 and a second driving component 122. The first driving component 121 is connected to the second driving component 122 to drive the second driving component 122 to move along the first direction X, and then the fluorescence detection module 11 completes the corresponding detection action at the corresponding position. The second driving component 122 is connected to the fluorescence detection module 11 to drive the fluorescence detection module 11 to move along the second direction Y.

[0066] During detection, the second driving component 122 drives the fluorescence detection module 11 to move along the second direction Y to respectively detect multiple samples placed along the second direction Y (multiple samples are arranged as a row of samples along the second direction Y), and then the first driving component 121 drives the fluorescence detection module 11 and the second driving component 122 to move along the first direction X to move the fluorescence detection module 11 to the next row of samples, and the above actions are repeated until the fluorescence detection module 11 has detected all the samples.

[0067] Optionally, the first driving assembly 121 may include a first driving member 1211, a first belt 1214, a first driving pulley 1212 and a first driven pulley 1213, wherein the first driving member 1211 is connected to the bearing seat 153, and the first driving member 1211 may be a motor. The first driving pulley 1212 and the first driven pulley 1213 are spaced apart along the first direction X and are rotatably connected to the bearing seat 153, and the first driving pulley 1212 is connected to the first driving member 1211, so that the first driving member 1211 drives the first driving pulley 1212 to rotate. The first belt 1214 is sleeved outside the first driving pulley 1212 and the first driven pulley 1213.

[0068] The first driving member 1211 works to drive the first active pulley 1212 to rotate, thereby driving the first belt 1214 sleeved outside the first active pulley 1212 to rotate. The second driving component 122 is connected to the first belt 1214, and then the second driving component 122 moves along the first direction X driven by the first belt 1214.

[0069] like Figure 2a-Figure 3b As shown, the second driving assembly 122 may include a mounting portion 1221, a second belt 1224, a second driving pulley 1222, and a second driven pulley 1223. The mounting portion 1221 is connected to the first driving assembly 121, and specifically, the mounting portion 1221 is connected to the first belt 1214. The second driving member is connected to the mounting portion 1221, and the second driving member may be a motor or the like. The second driving pulley 1222 and the second driven pulley 1223 are spaced apart and rotatably connected to the mounting portion 1221 along the second direction Y, and the second driving pulley 1222 is connected to the second driving member, so that the second driving member drives the second driving pulley 1222 to rotate. The second belt 1224 is sleeved outside the second driving pulley 1222 and the second driven pulley 1223.

[0070] The second driving member works to drive the second active pulley 1222 to rotate, thereby driving the second belt 1224 mounted outside the second active pulley 1222 to rotate, and the fluorescence detection module 11 is connected to the second belt 1224, and then the fluorescence detection module 11 is driven to move along the second direction Y under the rotation of the second belt 1224.

[0071] like Figure 2a and Figure 2c As shown, optionally, the second driving assembly 122 may further include at least two tensioning wheels 1225, which are disposed between the second driving pulley 1222 and the second driven pulley 1223 and are used to tension the second belt 1224, thereby improving the movement accuracy of the second belt 1224, thereby improving the position accuracy of the fluorescence detection module 11, and ensuring the accuracy of the detection result. In this embodiment, the number of tensioning wheels 1225 is three, and at least two tensioning wheels 1225 may have a spacing in the vertical direction Z to tension the second belt 1224.

[0072] The driving mechanism 12 may further include a first guide assembly 123, which is used to guide the second driving assembly 122, that is, when the second driving assembly 122 moves along the first direction X, the first guide assembly 123 can guide the second driving assembly 122 to improve the stability of the movement of the first guide assembly 123.

[0073] Optionally, the first guide assembly 123 may include a first guide rail 1231 and a first guide block 1232, the first guide rail 1231 extends along the first direction X, and illustratively, the first guide rail 1231 is connected to the bearing seat 153. The first guide block 1232 is slidably disposed on the first guide rail 1231, and the second drive assembly 122 is connected to the first guide block 1232, specifically, the mounting portion 1221 of the second drive assembly 122 is connected to the first guide block 1232. The number of the first guide rails 1231 may be one or two, and when the number of the first guide rails 1231 is two, the two first guide rails 1231 are disposed in parallel.

[0074] like Figure 3b , Figure 3a As shown, optionally, the driving mechanism 12 may further include a second guiding component 124, and the second guiding component 124 is used to guide the fluorescence detection module 11 to improve the stability of the movement of the detection module.

[0075] like Figure 3a and Figure 3b As shown, illustratively, the second guide assembly 124 may include a second guide rail 1241 and a second guide block 1242, the second guide rail 1241 extends along the second direction Y, and optionally, the second guide rail 1241 is connected to the mounting portion 1221. The second guide block 1242 is slidably disposed on the second guide rail 1241, and the fluorescence detection module 11 is connected to the second guide block 1242.

[0076] like Figure 1d , Figure 2a and Figure 3aAs shown, optionally, the high-speed fluorescence detection device may further include an in-situ detection mechanism 13 connected to the driving mechanism 12, and the in-situ detection mechanism 13 is used to detect whether there are samples at multiple preset positions, and the preset positions are used to place samples. In this embodiment, the preset position refers to the carrier position 1541, that is, the in-situ detection mechanism 13 is used to detect whether there is a sample on the carrier position 1541. If there is a sample on the carrier position 1541, the fluorescence detection module 11 detects the sample. If there is no sample on the carrier position 1541, the fluorescence detection module 11 does not need to detect the sample at the carrier position 1541. The in-situ detection mechanism 13 can pre-identify whether there is a sample. The pre-identification function of the in-situ detection mechanism 13 is integrated with the fluorescence detection function. As a new application method, it can effectively improve the detection efficiency and subsequent data processing efficiency, especially in high-throughput detection, which can effectively improve the subsequent data processing efficiency.

[0077] Optionally, the in-situ detection mechanism 13 may include a plurality of in-situ detection modules 131. The in-situ detection module 131 may be a photoelectric sensor or the like. Exemplarily, the plurality of in-situ detection modules 131 are sequentially arranged along the second direction Y. Furthermore, the plurality of in-situ detection modules 131 correspond one-to-one to each row of a plurality of carrier positions 1541 arranged along the second direction Y, and the in-situ detection mechanism 13 may detect at one time whether there is a sample on a row of carrier positions 1541 arranged along the second direction Y. In this way, the use frequency of each in-situ detection module 131 can be reduced, and the use time of the in-situ detection module 131 can be extended.

[0078] The driving mechanism 12 is used to drive the plurality of in-situ detection modules 131 to move along the first direction X, so that the in-situ detection mechanism 13 can detect whether there are samples on all the carrying positions 1541. At this time, the first driving assembly 121 is connected to the in-situ detection mechanism 13 to drive the in-situ detection module 131 to move along the first direction X. Further, the in-situ detection mechanism 13 is connected to the first belt 1214, such as the in-situ detection mechanism 13 is connected to the mounting portion 1221, and the in-situ detection mechanism 13 is connected to the first belt 1214 through the mounting portion 1221. At this time, the in-situ detection mechanism 13 is also connected to the first guide block 1232 through the mounting portion 1221, so that the first guide assembly 123 can also guide the in-situ detection mechanism 13.

[0079] Optionally, the in-situ detection mechanism 13 may further include a mounting plate 132, the mounting plate 132 is connected to the driving mechanism 12, and the in-situ detection module 131 is connected to the mounting plate 132. When there are multiple in-situ detection modules 131, the mounting plate 132 is connected to the mounting portion 1221 to move synchronously with the mounting portion 1221.

[0080] In other optional embodiments, the in-situ detection mechanism 13 may further include an in-situ detection module 131 to reduce the cost of the high-speed fluorescence detection device.

[0081] The following describes in detail the case where the presence detection mechanism 13 may include a presence detection module 131:

[0082] At this time, the second driving component 122 is connected to the in-situ detection module 131 to drive the in-situ detection module 131 to move along the second direction Y. Specifically, the in-situ detection module 131 is connected to the second belt 1224. The in-situ detection module 131 can be connected to the second belt 1224 through the mounting plate 132. Since the first driving component 121 is connected to the second driving component 122, the second driving component 122 is driven to move along the first direction, and the position detection module 131 is connected to the second driving component 122, the first driving component 121 also drives the position detection module 131 to move along the first direction, so as to realize the movement of the in-situ detection module 131 along the first direction and the second direction, and then detect whether there are samples on all the carrying positions 1541.

[0083] The second guide assembly 124 can guide the in-position detection mechanism 13 so that the in-position detection mechanism 13 can stably move in the second direction Y. Specifically, the second guide block 1242 is connected to the in-position detection mechanism 13 , and further, the in-position detection mechanism 13 is connected to the second guide block 1242 via the mounting plate 132 .

[0084] During the detection process, a round of detection is performed at preset time intervals, such as once every hour. For example, the detection can be started at every hour, such as at 1 o'clock, 2 o'clock, etc.

[0085] The high-speed fluorescence device provided in this embodiment has the characteristics of high throughput, rapidity and low cost, and its detection efficiency is not lower than that of array-type fluorescence detection (i.e., a one-to-one correspondence between multiple fluorescence detection modules and multiple samples). Moreover, the high-speed fluorescence device provided in this embodiment is a single fluorescence detection module 11, so its cost is lower.

[0086] like Figure 4 As shown, this embodiment also provides a fluorescence detection method. The high-speed fluorescence detection device can perform the fluorescence detection method. The fluorescence detection method may include:

[0087] In-situ detection: the driving mechanism 12 drives the in-situ detection mechanism 13 to move to the position, and the in-situ detection mechanism 13 detects whether there is a sample at the preset position (i.e., the carrying position 1541);

[0088] Fluorescence detection: If there is a sample at the preset position, the driving mechanism 12 drives the fluorescence detection module 11 to move to the position, and the fluorescence detection module 11 detects the sample.

[0089] In this embodiment, before performing fluorescence detection, it is first detected whether there is a sample on the carrier position 1541. If there is a sample on the carrier position 1541, the fluorescence detection module 11 detects the sample. If there is no sample on the carrier position 1541, the fluorescence detection module 11 does not detect at the carrier position 1541. The integration of in-situ detection and fluorescence detection can effectively improve the detection efficiency and subsequent data processing efficiency, especially in high-throughput detection, which can effectively improve the subsequent data processing efficiency. By moving the in-situ detection mechanism 13 and the fluorescence detection module 11, the fluorescence detection method can adapt to high-throughput detection.

[0090] When using the high-speed fluorescence detection device provided in this embodiment, the in-situ detection mechanism 13 can detect whether there is a sample on the carrier position 1541 before the fluorescence detection. If there is a sample on the carrier position 1541, the fluorescence detection module 11 detects the sample. If there is no sample on the carrier position 1541, the fluorescence detection module 11 does not need to detect the sample at the carrier position 1541, thereby effectively improving the detection efficiency and subsequent data processing efficiency. At the same time, the fluorescence detection module 11 can be moved, so that the samples can be detected quickly and separately. Due to the above reasons, the high-speed fluorescence detection device provided in this embodiment only takes about 10 minutes to complete the test of 400 hole positions, which greatly improves the detection efficiency.

[0091] Optionally, the fluorescence detection method may further include:

[0092] Return to the initial position: the in-situ detection mechanism 13 and the fluorescence detection module 11 are moved to the initial position. The above steps can be performed before the in-situ detection and the fluorescence detection, so that each detection starts from the initial position, thereby facilitating subsequent data processing. Optionally, the initial position can be the first bearing position located at the corner.

[0093] Optionally, before the in-situ detection and fluorescence detection, the following may also be included:

[0094] Instrument self-test: The in-situ detection mechanism 13 and the fluorescence detection module 11 move to the self-test position and perform self-test. Optionally, the self-test position can be the initial position of the in-situ detection mechanism 13 and the fluorescence detection module 11, so that the detection can be performed quickly after the self-test.

[0095] A calibration tube and an empty position (i.e., the bearing position 1541 is empty and no culture tube 100 is placed) can be set on the bearing position 1541 directly opposite to the initial position. When the in-situ detection mechanism 13 and the fluorescence detection module 11 are at the initial position, the fluorescence detection module 11 performs self-inspection and automatically sets the reference signal value by detecting the calibration tube. The in-situ detection mechanism 13 performs self-inspection at the empty position and automatically sets the reference signal value.

[0096] Optionally, there are multiple preset positions, that is, there are multiple bearing positions 1541, and the multiple preset positions are arranged in an array. The in-situ detection may include the in-situ detection mechanism 13 detecting a row of preset positions, which can reduce the use frequency of a single in-situ detection module 131 and increase the service life of a single in-situ detection module 131. After the in-situ detection mechanism 13 detects a row of bearing positions 1541, the fluorescence detection module 11 detects the samples on the bearing positions 1541 where the samples are placed one by one.

[0097] After the fluorescence detection module 11 detects the samples at all preset positions, after a preset time, the samples at all preset positions are detected again. Optionally, the preset time can be 1 hour, that is, every 1 hour, the fluorescence detection module 11 performs a round of data collection on all samples, and sends the detection results to the control mechanism for calculation and analysis until the sample incubation is completed and the detection of the samples is stopped.

[0098] Optionally, the culture tube 100 may be placed or removed during the detection process, and the fluorescence detection method may further include:

[0099] After the user places and / or takes out the tube, the position detection mechanism detects the position of the tube and / or takes out the tube, and issues a prompt if the culture tube 100 is not placed and / or taken out correctly. This confirms whether the user places / takes out the culture tube 100 correctly and avoids errors in the detection data.

[0100] Although the utility model has been described in detail above by means of general description, specific implementation methods and tests, it is obvious to those skilled in the art that some modifications or improvements can be made to the utility model. Therefore, these modifications or improvements made without departing from the spirit of the utility model are within the scope of protection claimed by the utility model.

Claims

1. A high-speed fluorescence detection device, characterized in that: It comprises a fluorescence detection module (11) and a driving mechanism (12), wherein the driving mechanism (12) is used to drive the fluorescence detection module (11) to move so that the fluorescence detection module (11) can respectively detect a plurality of samples located at different positions.

2. The high-speed fluorescence detection device according to claim 1, characterized in that: The driving mechanism (12) is used to drive the fluorescence detection module (11) to move back and forth along a first direction and a second direction, the first direction and the second direction being perpendicular.

3. The high-speed fluorescence detection device according to claim 1, characterized in that: The high-speed fluorescence detection device further comprises an in-situ detection mechanism (13) connected to the driving mechanism (12), wherein the in-situ detection mechanism (13) is used to detect whether samples exist at a plurality of preset positions, wherein the preset positions are used to place samples.

4. The high-speed fluorescence detection device according to claim 3, characterized in that: The in-situ detection mechanism (13) comprises one or more in-situ detection modules (131).

5. The high-speed fluorescence detection device according to claim 4, characterized in that: When there are multiple on-site detection modules (131), the multiple on-site detection modules (131) are arranged in sequence along the second direction, and the driving mechanism (12) is used to drive the multiple on-site detection modules (131) to move along the first direction.

6. The high-speed fluorescence detection device according to claim 1, characterized in that: The high-speed fluorescence detection device further comprises a carrying portion (154), wherein the carrying portion (154) is provided with a plurality of carrying positions (1541), and the carrying positions (1541) are used to carry samples; optionally, the plurality of carrying positions (1541) are arranged in an array along the first direction and the second direction.

7. The high-speed fluorescence detection device according to claim 4 or 5, characterized in that: The driving mechanism (12) comprises a first driving component (121) and a second driving component (122), wherein the first driving component (121) is connected to the second driving component (122) to drive the second driving component (122) to move along a first direction; The second driving component (122) is connected to the fluorescence detection module (11) to drive the fluorescence detection module (11) to move along a second direction; and / or When there are multiple in-position detection modules (131), the first driving component (121) is connected to the in-position detection mechanism (13) to drive the in-position detection module (131) to move along a first direction; when there is only one in-position detection module (131), the second driving component (122) is connected to the in-position detection module (131) to drive the in-position detection module (131) to move along a second direction.

8. The high-speed fluorescence detection device according to claim 7, characterized in that: The high-speed fluorescence detection device further comprises a bearing seat (14); the first driving component (121) comprises: A first driving member (1211) connected to the bearing seat (14); A first driving pulley (1212) and a first driven pulley (1213) are spaced apart in a first direction and rotatably connected to the bearing seat (14), and the first driving pulley (1212) is connected to the first driving member (1211); A first belt (1214) is sleeved outside the first driving pulley (1212) and the first driven pulley (1213), and the second driving component (122) is connected to the first belt (1214); And / or, the second drive assembly (122) comprises: A mounting portion (1221), connected to the first driving assembly (121); A second driving member connected to the mounting portion (1221); A second driving pulley (1222) and a second driven pulley (1223) are spaced apart in a second direction and rotatably connected to the mounting portion (1221), and the second driving pulley (1222) is connected to the second driving member; A second belt (1224) is sleeved outside the second active pulley (1222) and the second driven pulley (1223), and the fluorescence detection module (11) is connected to the second belt (1224); The in-situ detection module (131) is connected to the first belt (1214) or the second belt (1224).

9. The high-speed fluorescence detection device according to claim 8, characterized in that: The second driving assembly (122) further comprises at least two tensioning wheels (1225), wherein the tensioning wheels (1225) are arranged between the second driving pulley (1222) and the second driven pulley (1223), and are used for tensioning the second belt (1224).

10. The high-speed fluorescence detection device according to claim 7, characterized in that: The driving mechanism (12) further comprises a first guide component (123), wherein the first guide component (123) is used to guide the second driving component (122); and / or The driving mechanism (12) further comprises a second guiding component (124), wherein the second guiding component (124) is used to guide the fluorescence detection module (11); When there are multiple in-situ detection modules (131), the first guide component (123) can guide the in-situ detection mechanism (13); when there is only one in-situ detection module (131), the second guide component (124) can guide the in-situ detection mechanism (13).

11. The high-speed fluorescence detection device according to claim 10, characterized in that: The first guide assembly (123) comprises a first guide rail (1231) and a first guide block (1232), the first guide rail (1231) extends along a first direction, the first guide block (1232) is slidably disposed on the first guide rail (1231), and the second driving assembly (122) is connected to the first guide block (1232); and / or The second guide assembly (124) comprises a second guide rail (1241) and a second guide block (1242), the second guide rail (1241) extends along a second direction, the second guide block (1242) is slidably disposed on the second guide rail (1241), and the fluorescence detection module (11) is connected to the second guide block (1242); When there are multiple in-place detection modules (131), the first guide block (1232) is connected to the in-place detection mechanism (13); when there is only one in-place detection module (131), the second guide block (1242) is connected to the in-place detection mechanism (13).

12. The high-speed fluorescence detection device according to claim 4 or 5, characterized in that: The in-situ detection mechanism (13) further comprises a mounting plate (132), wherein the mounting plate (132) is connected to the driving mechanism (12), and the in-situ detection module (131) is connected to the mounting plate (132).

13. The high-speed fluorescence detection device according to claim 2, characterized in that: The first direction and the second direction are located in a horizontal plane.

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