Specimen detection device

Abstract

The utility model relates to a specimen detection device which comprises a sampling assembly and an insertion plate assembly, and further comprises a detection assembly and a lysis solution assembly. The detection assembly is formed by nesting an inner bottle body and an outer bottle body, the insertion plate assembly is arranged between the inner bottle body and the outer bottle body, the lysis solution assembly is arranged in a cavity of the inner bottle body, the lysis solution assembly comprises a lysis solution bottle and a sealing film covering the lysis solution bottle, and an overflow hole is longitudinally formed in the inner bottle body from the bottom; and when the bottom of the lysate bottle is in contact with the bottom of the inner bottle body, the height of the lysate bottle is higher than that of the lysate bottle. Aiming at a sample which is small in sampling amount and needs to be detected after being mixed with the lysis solution, the device is integrally designed, so that the mixing operation of the sample and the lysis solution is more convenient, the operation pollution is reduced, and the accuracy is improved.

Description

Technical Field

[0001] The utility model belongs to the technical field of sample detection, and in particular relates to a sample detection device. Background Art

[0002] In the prior art, when performing immunochromatographic detection on some sample types (such as colloidal gold immunochromatography), the sampling amount is too small. Taking saliva specimens as an example, when only saliva specimens are used for detection, most people have a small amount of saliva, which cannot reach the amount of saliva required for detection, affecting the accuracy of the test results. When using lysis solution for lysis, most of the existing device technologies now require the saliva specimen and the lysis solution to be mixed separately first, and then added dropwise to the detection device. The operation is relatively complicated, and the risk of sample contamination is also increased during the dropwise transfer process. Summary of the invention

[0003] In view of the deficiencies of the prior art and products, the purpose of the utility model is to provide a specimen detection device, comprising a sampling component and an insert plate component, and also comprising a detection component and a lysis liquid component; the detection component is composed of an inner bottle body and an outer bottle body nested together; the insert plate component is arranged between the inner bottle body and the outer bottle body, the lysis liquid component is arranged in the cavity of the inner bottle body, the lysis liquid component comprises a lysis liquid bottle and a sealing film covering the lysis liquid bottle, the inner bottle body is provided with an overflow hole in the longitudinal direction from the bottom, and when the bottom of the lysis liquid bottle contacts the bottom of the inner bottle body, the height of the overflow hole is higher than the height of the lysis liquid bottle.

[0004] Preferably, the open end of the outer bottle body is provided with an exhaust port, and when the inner bottle body and the outer bottle body are nested and connected, the exhaust port can realize the connection between the intermediate layer of the inner bottle body and the atmosphere. Preferably, the number of the exhaust ports is one or more.

[0005] Preferably, the inner bottle body is configured into two sections, the upper section has a diameter larger than the lower section; the lower section has a height lower than the height of the lysis liquid bottle; the diameter of the lysis liquid bottle mouth is larger than the bottle body diameter of the lysis liquid bottle, and the diameter of the lysis liquid bottle mouth is between the diameter of the lower section and the diameter of the upper section.

[0006] Preferably, a guide groove and a reaction chamber are provided at the bottom of the outer bottle body, the drainage surface of the guide groove gradually decreases in the direction from the drainage starting end to the drainage ending end, and the reaction chamber is arranged at the drainage ending end of the drainage surface of the guide groove.

[0007] Preferably, the detection starting end of the plug board assembly is located in the reaction chamber.

[0008] Preferably, the overflow hole is arranged on the same side of the drainage surface of the guide groove as the drainage starting end.

[0009] Preferably, the plug board assembly is provided with a plug board, a detection reagent strip, and a panel label in sequence from the inside to the outside.

[0010] Preferably, the sampling component is provided with a component handle, a sealing ring, a sample collection sponge, and a sponge sleeve column from top to bottom, and the sponge sleeve column is placed above the sealing film in the initial state.

[0011] Preferably, the diameter of the sponge sleeve column is smaller than the diameter of the collection sponge, the diameter of the collection sponge is smaller than the inner diameter of the lysis solution bottle, and the length of the sample collection sponge is greater than the height of the lysis solution bottle.

[0012] The utility model has the following beneficial effects:

[0013] 1) The operation is simpler, and there is no need for the additional transfer operation step after the sample is mixed with the lysis solution, which makes the operation more convenient and user-friendly; 2) The sample is enclosed in the device during the entire operation process to reduce operational contamination; 3) The sampling device is an independent component and can be packaged separately to avoid contamination during the production process. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 It is a schematic diagram of the sampling component and the detection component of the utility model;

[0015] Figure 2 It is a structural sectional view of the utility model;

[0016] Figure 3 It is a schematic diagram of the explosion of the structure of the utility model;

[0017] Figure 4 This is a schematic diagram of the structure of the inner bottle body of the utility model;

[0018] Figure 5 This is a schematic top view of the structure of the outer bottle body of the utility model;

[0019] Figure 6 It is a structural cross-sectional view of the utility model in the initial process;

[0020] Figure 7 It is a structural sectional view of the utility model during use.

[0021] Figure numerals: 100, sampling assembly; 101, handle; 1011, hand holding end; 1012, sampling rod; 102, sealing ring; 103, sample collection sponge; 104, sponge sleeve column; 200, detection assembly; 201, inner bottle body; 2011, overflow hole; 2012, cavity; 202, outer bottle body; 2021, guide groove; 2022, reaction chamber; 2023, exhaust port; 300, plug-in board assembly; 301, plug-in board; 302, detection reagent strip; 303, panel label; 400, lysis solution assembly; 401, sealing film; 402, lysis solution bottle. DETAILED DESCRIPTION

[0022] The device can also be used for immunochromatographic testing when the amount of other specimens collected is too small. The specific implementation method takes saliva specimen testing as an example. The structure of the utility model is further described below in conjunction with the accompanying drawings.

[0023] like Figure 1-5 As shown, a specimen detection device includes a sampling assembly 100, a plug-in assembly 300, a detection assembly 200 and a lysis solution assembly 400. The detection assembly 200 is composed of an inner bottle body 201 and an outer bottle body 202, and the plug-in assembly 300 and the lysis solution assembly 400 are both arranged in the detection assembly 200. Specifically, the plug-in assembly 300 is arranged between the inner bottle body 201 and the outer bottle body 202, and the lysis solution assembly 400 is arranged in the cavity 2012 of the inner bottle body 201, and the cavity 2012 is cylindrical. The inner bottle body 201 and the outer bottle body 202 are nested and connected by a snap-fit ​​member 2013 arranged below the open end of the inner bottle body 201.

[0024] In some embodiments, the inner bottle body 201 and the outer bottle body 202 can be nested and connected by welding, bonding, etc. In some embodiments, the outer bottle body 202 is preferably a transparent structure, which facilitates observation of the detection situation and the detection results. The transparent structure material includes but is not limited to plastic, PVC, etc.

[0025] When the inner bottle body 201 and the outer bottle body 202 are nested and connected, in order to connect the intermediate layer between the two with the atmosphere, which is beneficial to the chromatography reaction during detection, an exhaust port is provided to achieve this function. In this optimal solution, the position and number of the exhaust ports are two exhaust ports 2023 arranged at the circumferential opening end of the outer bottle body 202 facing each other.

[0026] The lysate assembly 400 includes a lysate bottle 402 and a sealing film 401, wherein the sealing film 401 covers the lysate bottle 402 (used to seal the lysate before being punctured). The inner bottle body 201 is provided with an overflow hole 2011 in the longitudinal direction from the bottom, and when the bottom of the lysate bottle 402 contacts the bottom of the inner bottle body 201, the height of the overflow hole 2011 is higher than the height of the lysate bottle 402.

[0027] In this optimal solution, the inner bottle body 201 is divided into two sections, the upper section has a larger diameter than the lower section; the lower section is lower than the height of the lysis liquid bottle 402. The diameter of the mouth of the lysis liquid bottle 402 is larger than the body diameter of the lysis liquid bottle 402, and the diameter of the mouth of the lysis liquid bottle 402 is between the diameter of the lower section and the diameter of the upper section (i.e., the diameters are: upper section diameter > mouth diameter of the lysis liquid bottle 402 ≥ lower section diameter > body diameter of the lysis liquid bottle 402). This allows the bottom of the lysis liquid bottle 402 to be fixed in place when in contact with the bottom of the inner bottle body 201, and the mouth of the lysis liquid bottle 402 can be snapped or overlapped at the junction of the lower section and the upper section (such as Figure 6As shown), the lysis solution bottle 402 is prevented from shaking or deflecting in the cavity 2012 of the inner bottle body 201 during transportation and use.

[0028] The bottom of the outer bottle body 202 is provided with a guide groove 2021 and a reaction chamber 2022. The guide surface of the guide groove 2021 is an inclined surface, that is, it gradually decreases in the direction from the guide start end to the guide end end. The reaction chamber 2022 is arranged at the guide end end of the guide surface of the guide groove 2021. The detection start end of the plugboard assembly 300 is located in the reaction chamber 2022.

[0029] The overflow hole 2011 is arranged on the same side as the drainage start end of the drainage surface of the guide groove 2021. When the inner bottle body 201 is nested and connected to be placed in the outer bottle body 202, the distance between the drainage start end of the drainage surface of the guide groove 2021 and the bottom of the outer bottle body 202 is less than or equal to the distance between the bottom of the inner bottle body 201 and the bottom of the outer bottle body 202. Most preferably, the distance between the drainage start end of the drainage surface of the guide groove 2021 and the bottom of the outer bottle body 202 is equal to the distance between the bottom of the inner bottle body 201 and the bottom of the outer bottle body 202, that is, the mixed solution (i.e., the mixed solution of the lysate and the specimen) flowing out of the overflow hole 2011 flows from the drainage start end into the reaction chamber 2022 located at the drainage end end.

[0030] Furthermore, in some embodiments, the drainage surface of the guide groove 2021 is made of hydrophobic material, so that the mixed solution overflowing from the overflow hole 2011 can flow more effectively and gather into the reaction chamber 2022, so that even if the amount of collected specimens is small, the accuracy of the test results can be guaranteed. In some embodiments, the lysate bottle 402 is made of high barrier material to prevent the lysate from volatilizing during storage, resulting in a reduction in the lysate or product failure.

[0031] The sampling assembly 100 is provided with an assembly handle 101, a sealing ring 102, a sample collection sponge 103, and a sponge sleeve 104 from top to bottom (i.e., from the hand-held end to the specimen sampling end). In the initial state, the sponge sleeve 104 is placed above the sealing film 401. When the sampling assembly 100 is inserted into the inner bottle body 201 as a whole, the outer edge of the sealing ring 102 contacts the inner side wall of the inner bottle body 201 to achieve sealing, and also prevents the mixed liquid from overflowing to the upper end of the inner bottle body 201 when squeezed downward.

[0032] The component handle 101 is composed of a hand-holding end 1011 and a sampling rod 1012; the hand-holding end 1011 is designed to have the same width as the fingerprint of the finger, so that it is more secure and more convenient to hold during sampling. The diameter of the sponge sleeve 104 is smaller than the diameter of the collection sponge 103, and the diameter of the collection sponge 103 is smaller than the inner diameter of the lysis solution bottle 402. The sample collection sponge 103 is hollow, and the diameter of the hollow area is equal to the diameter of the sponge sleeve 104, so that the sample collection sponge 103 is sleeved on the sponge sleeve 104, and the combination of the two can also be inserted into the lysis solution bottle 402. The length of the sample collection sponge 103 is greater than the height of the lysis solution bottle 402, so that the sample collection sponge 103 can be fully inserted into the lysis solution bottle 402, and the saliva specimen can be squeezed out more fully. The sampling rod 1012 is hollow. After the sample collection sponge 103 is sleeved on the sponge sleeve 104, the side of the sponge sleeve 104 away from the puncture end is inserted into the hollow area of ​​the sampling rod 1012, and a sealed connection is achieved by conical expansion (such as Figure 6 shown).

[0033] The plugboard assembly 300 is provided with a plugboard 301, a detection reagent strip 302, and a panel label 303 from the inside to the outside. The detection reagent strip 302 is placed in the strip-shaped installation slot provided in the plugboard 301, and the panel label 303 covers the detection reagent strip 302. The middle of the panel label 303 is hollowed out and corresponds to the detection result area of ​​the detection reagent strip 302 (that is, the color development results of the detection line and the quality control line can be observed).

[0034] The detection reagent strip is a detection reagent strip used for immunochromatography reactions such as colloidal gold immunochromatography and latex immunochromatography. It consists of a PVC base plate and a sample pad, a marking pad, a nitrocellulose membrane and a water-absorbing pad connected in sequence on the PVC base plate. The detection starting end of the plug-in assembly 300 is the sample pad end of the detection reagent strip 302.

[0035] In some embodiments, the detection reagent strip 302 can be one or more (such as 1-18), preferably multiple, that is, the insert 301 is provided with multiple strip-shaped mounting grooves, each strip-shaped mounting groove is installed with a detection reagent strip 302, and the number of the insert 301 and the panel label 303 corresponds to the number of the detection reagent strips 302, and each panel label 303 is marked with a different name of the substance to be tested on the upper side, and the same saliva sample to be tested is tested for multiple substances to be tested through the color development results of the chromatography reaction. In some embodiments, the substance to be tested can be any substance to be analyzed, including but not limited to: human chorionic gonadotropin (hCG), luteinizing hormone (LH), viruses and any abused drugs, and any other analytes suitable for lateral flow test can be detected by this device.

[0036] like Figure 6 and Figure 7 As shown, the working principle of the utility model is as follows:

[0037] After the sample collection sponge 103 in the sampling assembly 100 collects oral saliva, it is inserted into the cavity 2012 of the bottle body 201 in the detection assembly 200. Figure 6 In the state shown, the end sponge sleeve column 104 of the sampling component 100 contacts the sealing film 401 in the lysis solution component 400 , and the sealing ring 102 in the sampling component 100 forms a seal with the inner bottle body 201 of the detection component 200 .

[0038] After the sampling assembly 100 is further inserted, the sponge sleeve 104 at the end of the sampling assembly 100 pierces the sealing film 401, and the sample collection sponge 103 is inserted into the lysis bottle 402 of the lysis solution assembly 400. The sample collection sponge 103 is mixed and squeezed with the lysis solution in the lysis solution bottle 402, so that the saliva specimen in the sample collection sponge 103 is squeezed out and mixed with the lysis solution in the lysis solution bottle 402. As the insertion stroke increases, the internal pressure of the lysis bottle 402 increases, and the mixed liquid of the specimen and the lysis solution flows into the outer bottle body 202 through the overflow hole 2011, flows from the drainage starting end to the drainage ending end through the guide groove 2021 at the bottom of the outer bottle body 202, and then gathers in the reaction chamber 2022.

[0039] As the liquid capacity of the reaction chamber 2022 increases, the internal and external pressures are balanced through the exhaust port 2023 at the upper end of the outer bottle 202. The test strip 302 in the plug board 301 absorbs the liquid in the reaction chamber 2022, and starts the immunochromatographic test reaction. The test result is determined by observing the color development of the test strip 302.

Claims

1. A specimen detection device, comprising a sampling assembly (100) and a plug assembly (300), characterized in that: The invention also comprises a detection component (200) and a lysis liquid component (400); the detection component (200) is composed of an inner bottle body (201) and an outer bottle body (202) which are nested; the plug plate component (300) is arranged between the inner bottle body (201) and the outer bottle body (202); the lysis liquid component (400) is arranged in the cavity (2012) of the inner bottle body (201); the lysis liquid component (400) comprises a lysis liquid bottle (402) and a sealing film (401) covering the lysis liquid bottle (402); the inner bottle body (201) is provided with an overflow hole (2011) in the longitudinal direction from the bottom; when the bottom of the lysis liquid bottle (402) contacts the bottom of the inner bottle body (201), the height of the overflow hole (2011) is higher than the height of the lysis liquid bottle (402).

2. A sample detection device according to claim 1, characterized in that: An exhaust port (2023) is provided at the open end of the outer bottle body (202).

3. A sample detection device according to claim 2, characterized in that: The number of the exhaust ports (2023) is one or more.

4. A sample detection device according to claim 1, characterized in that: The inner bottle body (201) is configured to be divided into two sections, the diameter of the upper section is larger than the diameter of the lower section; the height of the lower section is lower than the height of the lysate bottle (402); the diameter of the mouth of the lysate bottle (402) is larger than the diameter of the bottle body of the lysate bottle (402), and the diameter of the mouth of the lysate bottle (402) is between the diameter of the lower section and the diameter of the upper section.

5. A sample detection device according to claim 1, characterized in that: The bottom of the outer bottle body (202) is provided with a guide groove (2021) and a reaction chamber (2022); the drainage surface of the guide groove (2021) gradually decreases in the direction from the drainage starting end to the drainage ending end; and the reaction chamber (2022) is arranged at the drainage ending end of the drainage surface of the guide groove (2021).

6. A sample detection device according to claim 5, characterized in that: The detection starting end of the plugboard assembly (300) is located in the reaction chamber (2022).

7. A specimen detection device according to claim 5, characterized in that: The overflow hole (2011) is arranged on the same side as the drainage starting end of the drainage surface of the guide groove (2021).

8. A sample detection device according to any one of claims 1 to 7, characterized in that: The plug board assembly (300) is provided with a plug board (301), a detection reagent strip (302), and a panel label (303) in sequence from the inside to the outside.

9. A specimen detection device according to claim 8, characterized in that: The sampling component (100) is provided with a component handle (101), a sealing ring (102), a sample collection sponge (103), and a sponge sleeve column (104) from top to bottom. In the initial state, the sponge sleeve column (104) is placed above the sealing film (401).

10. A sample detection device according to claim 9, characterized in that: Sponge sleeve column (104) The diameter is smaller than the diameter of the collection sponge (103), and the diameter of the collection sponge (103) is smaller than the inner diameter of the lysate bottle (402), The length of the sample collection sponge (103) is greater than the height of the lysis solution bottle (402).

Images