Multi-modal imaging system and time difference incubator
By designing a multimodal imaging system in a time difference in incubator, dynamic adjustment of lighting and imaging modules is achieved, and the problem of single imaging mode and low adaptability of traditional time difference in incubator is solved, improving the accuracy of embryo evaluation and screening and the adaptability of equipment.
Patent Information
- Application Number
- CN202421465862.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-06-25
- Publication Date
- 2025-03-25
- Estimated Expiration
- 2034-06-25
AI Technical Summary
Due to product specification limitations, traditional jet lag incubators adopt fixed lighting systems and imaging systems. The imaging mode is single and the adaptability is low, resulting in redundant waste of labor and equipment.
A multimodal imaging system is designed, including an adjustable lighting module and an imaging module, and free switching between bright field lighting, oblique lighting and Hoffman imaging modes is achieved through dynamic adjustment of movable plastic shaping devices and objective modulation board.
Through the dynamic switching function of the multimodal imaging system, the redundancy of microscopic imaging equipment is reduced according to the sample type and imaging requirements, the adaptability is improved, and the accuracy of embryo evaluation and screening is improved.
Smart Images
Figure CN222672040U_ABST
Abstract
Description
Technical Field
[0001] The utility model belongs to the technical field of medical equipment, and in particular relates to a multimodal imaging system and a time-difference incubator. Background Art
[0002] As the demand for assisted reproductive market continues to grow, embryo culture technology has received more attention. Traditional culture methods are inefficient and require a lot of human resources. In order to solve this problem, the time difference culture system has become an emerging embryo culture and screening method. The time difference incubator can dynamically observe the development process of the embryo, evaluate the development of the embryo, and select the best embryos for transplantation, thereby effectively improving the success rate of embryo culture, improving the implantation rate, and shortening the patient's successful conception time.
[0003] The microscopic imaging module in the time-lapse incubator is the most critical part. Only by taking three-dimensional and layered images of biological tissues with the camera can the morphology and development of biological tissues be accurately judged, and the accuracy of embryo evaluation and screening be improved. In related technologies, traditional time-lapse incubators will respectively set up lighting systems and imaging systems on both sides of the culture dish, and corresponding lenses will be set in the lighting system and imaging system to realize the imaging of highly transparent embryo samples using oblique illumination or Hoffman imaging technology.
[0004] Due to the limitations of product specifications, time-difference incubators in related technologies usually use fixed lighting systems and imaging systems to perform microscopic imaging of samples, and the imaging mode is single. Experimenters often need to culture and perform microscopic imaging in multiple time-difference incubators according to the type of culture sample, which has low adaptability and causes redundant waste of manpower and equipment. Utility Model Content
[0005] The embodiment of the utility model provides a multi-modal imaging system and a time-difference incubator, which can realize the free switching of multiple imaging modes according to different culture samples, thereby improving adaptability. The technical solution is as follows:
[0006] In a first aspect, an embodiment of the utility model provides a multimodal imaging system, comprising: an illumination module, an imaging module and a culture dish,
[0007] The lighting module is fixedly arranged above the culture dish, and comprises a light source, a focusing system and a movable shaping device, wherein the light source and the focusing system are arranged at intervals in a direction close to the culture dish, a slit is arranged on the movable shaping device, and the movable shaping device is movably connected to the lighting module to have a first working position located at a front focal plane of the focusing system, and a second working position adjusted away from the light path;
[0008] The imaging module is arranged below the culture dish, and comprises an objective lens, a tube lens, an image collector and an objective lens modulation plate. The objective lens, the tube lens and the image collector are arranged at intervals in a direction away from the culture dish. The objective lens modulation plate matches the slit. The objective lens modulation plate is movably connected to the imaging module to have a third working position located between the objective lens and the tube lens, and a fourth working position adjusted away from the optical path.
[0009] Optionally, the movable shaping device is configured to be capable of biaxial movement in a horizontal direction.
[0010] Optionally, the clear aperture of the slit is adjustable.
[0011] Optionally, a polarizer is arranged between the light source and the active shaping device, and an analyzer matching the polarizer is arranged at the slit of the active shaping device.
[0012] Optionally, the movable shaping device is detachably connected to the illumination module; and the objective lens modulation plate is detachably connected to the imaging module.
[0013] Optionally, the light source is a light emitting diode light source, and the wavelength range of the light source is 400 to 700 nm.
[0014] Optionally, the image collector is a charge coupled device camera or a complementary metal oxide semiconductor camera.
[0015] In a second aspect, an embodiment of the utility model provides a time-difference incubator, comprising the multimodal imaging system as described in the first aspect above.
[0016] The beneficial effects brought by the technical solution provided by the embodiment of the utility model include at least:
[0017] By adopting the multimodal imaging system provided by the embodiment of the utility model, by respectively arranging adjustable active shaping devices in the illumination module and the imaging module, and by adjusting the active shaping devices and the position relative to the imaging light path, the multimodal imaging system can dynamically switch the imaging mode relative to the culture dish arranged inside, and freely switch between bright field illumination, oblique illumination imaging and Hoffman imaging according to different sample types and imaging requirements, thereby reducing the redundancy of microscopic imaging equipment and improving adaptability. BRIEF DESCRIPTION OF THE DRAWINGS
[0018] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings required for use in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.
[0019] Figure 1 It is a structural schematic diagram of a multimodal imaging system provided by an embodiment of the utility model;
[0020] Figure 2 It is a structural schematic diagram of a multimodal imaging system provided by an embodiment of the utility model in a bright field illumination mode;
[0021] Figure 3 It is a structural schematic diagram of a multimodal imaging system in an oblique illumination mode provided by an embodiment of the utility model;
[0022] Figure 4 It is a structural schematic diagram of an active shaping device provided by an embodiment of the utility model;
[0023] Figure 5 The corresponding embodiment of the utility model provides Figure 4 Schematic diagram of the structure of the objective lens modulation plate;
[0024] Figure 6 It is a structural schematic diagram of a multimodal imaging system provided by an embodiment of the utility model in a Hoffman illumination mode.
[0025] In the figure:
[0026] 1-illumination assembly; 2-imaging module; 3-culture dish; 11-light source; 12-focusing system; 14-active shaping device; 15-polarizer; 16-analyzer; 21-objective lens; 22-tube lens; 23-image collector; 24-objective lens modulation plate; 141-slit. DETAILED DESCRIPTION
[0027] In order to make the purpose, technical solution and advantages of the present invention more clear, the implementation mode of the present invention will be further described in detail below with reference to the accompanying drawings.
[0028] The microscopic imaging module in the time-lapse incubator is the most critical part. Only by taking three-dimensional and layered images of biological tissues with the camera can the morphology and development of biological tissues be accurately judged, and the accuracy of embryo evaluation and screening be improved. In related technologies, traditional time-lapse incubators will respectively set up lighting systems and imaging systems on both sides of the culture dish, and corresponding lenses will be set in the lighting system and imaging system to realize the imaging of highly transparent embryo samples using oblique illumination or Hoffman imaging technology.
[0029] Due to the limitations of product specifications, time-difference incubators in related technologies usually use fixed lighting systems and imaging systems to perform microscopic imaging of samples, and the imaging mode is single. Experimenters often need to culture and perform microscopic imaging in multiple time-difference incubators according to the type of culture sample, which has low adaptability and causes redundant waste of manpower and equipment.
[0030] Figure 1 It is a structural schematic diagram of a multimodal imaging system provided by an embodiment of the utility model; Figure 2 It is a structural schematic diagram of a multimodal imaging system provided by an embodiment of the utility model in a bright field illumination mode; Figure 3 It is a structural schematic diagram of a multimodal imaging system in an oblique illumination mode provided by an embodiment of the utility model; Figure 4 It is a structural schematic diagram of an active shaping device provided by an embodiment of the utility model; Figure 5 The corresponding embodiment of the utility model provides Figure 4 Schematic diagram of the structure of the objective lens modulation plate; Figure 6 Schematic diagram of the structure of the multimodal imaging system provided by the embodiment of the utility model in the Hoffman illumination mode. Figures 1 to 6 As shown, through practice, an embodiment of the utility model provides a multimodal imaging system, including an illumination module 1, an imaging module 2 and a culture dish 3.
[0031] The lighting module 1 is fixedly arranged above the culture dish 3, and includes a light source 11, a focusing system 12, and an active shaping device 14. The light source 11 and the focusing system 12 are arranged at intervals in a direction close to the culture dish 3. A slit 141 is provided on the active shaping device 14, and the active shaping device 14 is movably connected to the lighting module 1 to have a first working position located at the front focal plane of the focusing system 12, and a second working position adjusted away from the light path.
[0032] The imaging module 2 is disposed below the culture dish 3, and includes an objective lens 21, a tube lens 22, an image collector 23, and an objective lens modulation plate 24. The objective lens 21, the tube lens 22, and the image collector 23 are arranged at intervals in a direction away from the culture dish 3. The objective lens modulation plate 24 matches the slit 141, and the objective lens modulation plate 24 is movably connected to the imaging module 2 to have a third working position located between the objective lens 21 and the tube lens 22, and a fourth working position adjusted away from the optical path.
[0033] In an embodiment of the utility model, the multimodal imaging system is arranged in a time-difference incubator. When microscopic imaging of embryo samples is required, the staff first loads the culture dish 3 containing the embryo samples into the time-difference incubator. When the culture dish 3 is installed in place, it is between the lighting module 1 and the imaging module 2.
[0034] refer to Figure 1 to Figure 2When imaging conventional biological tissues and cells, the active shaping device 14 is adjusted to the second working position manually or by a related driving structure, and the objective lens modulation plate 24 is adjusted to the fourth working position, so that both the active shaping device 14 and the objective lens modulation plate 24 are removed from the illumination of the light source 11 and the imaging path of the image collector 23, forming a bright field illumination mode. At this time, the illumination module 1 is started, and the light source 11 emits light, which is focused on the sample in the culture dish 3 after passing through the focusing system 12 to achieve bright field illumination, and the imaging module 2 composed of the objective lens 21, the tube lens 22 and the image collector 23 under the culture dish 3 is used to image the sample in the culture dish 3; or,
[0035] refer to Figure 3 to Figure 4 When imaging biological tissues and cells with a certain degree of transparency, the active shaping device 14 is adjusted to the first working position, and the objective lens modulation plate 24 is adjusted to the fourth working position, so that the active shaping device 14 is adjusted to the optical path of the light source 11. The illumination module 1 is started, and the light source 11 emits light, which is focused on the sample of the culture dish 3 after passing through the active shaping device 14 and the focusing system 12. The slit 141 on the active shaping device 14 is used to block the light, and only part of the light of the illumination spot is allowed to pass through the slit 141, and then is focused on the sample of the culture dish 3 through the second focusing system 13 to achieve oblique illumination. Finally, the image collector 23 is used to perform high-resolution imaging of the sample. Compared with the bright field imaging mode, the collected pictures have a more three-dimensional sense and a sense of layering.
[0036] refer to Figures 4 to 6 When imaging cells with high transparency, the conventional bright field imaging method has a poor imaging effect on embryos, lacks a sense of three-dimensionality and layering, and cannot accurately evaluate and screen the developmental state of the embryos. At this time, by adjusting the active shaping device 14 to the first working position and the objective lens modulation plate 24 to the third working position, the active shaping device 14 and the objective lens modulation plate 24 are adjusted to the light path of the light source 11. Start the illumination module 1, use the light source 11 to emit light, and after passing through the active shaping device 14 and the focusing system 12, the light is focused on the transparent sample of the culture dish 3, causing refraction and diffraction, and then passing through the objective lens 21 and the objective lens modulation plate 14, and using the structure of the slit 141 and the objective lens modulation plate 242 to adjust to the corresponding optimal imaging position, forming a Hoffman imaging structure. Three areas of dark, gray and light are formed on the objective lens modulation plate 24, and finally the refracted and diffracted light at different angles hits different areas of the objective lens modulation plate 24, so that the transparent sample produces light and dark changes, and the imaging has a relief effect. Finally, the sample is imaged by the image collector 23 to improve the three-dimensional sense and layering of the imaging. On the basis of oblique illumination, the imaging capability of transparent samples is further improved by using the coordinated structure of the objective lens 21 and the objective lens modulation plate 242, thereby improving the three-dimensional sense and layering of the microscopic imaging.
[0037] By adopting the multimodal imaging system provided by the embodiment of the utility model, by respectively arranging an adjustable active shaping device 14 and an objective lens modulation plate 24 in the illumination module 1 and the imaging module 2, and by adjusting the positions of the active shaping device 14 and the objective lens modulation plate 24 relative to the imaging light path, the multimodal imaging system can dynamically switch the imaging mode relative to the culture dish 3 arranged inside, and freely switch between bright field illumination, oblique illumination imaging and Hoffman imaging according to different types of samples and imaging requirements, thereby reducing the redundancy of microscopic imaging equipment and improving adaptability.
[0038] Optionally, the active shaping device 14 is configured to be capable of biaxial movement in the horizontal direction. For example, in the embodiment of the present utility model, within a certain range, the larger the illumination angle of the light spot at the sample on the culture dish 3 is, the stronger the imaging resolution is. The active shaping device 14 located at the first working position is moved left and right in the horizontal direction by external manual adjustment or a corresponding driving mechanism, so as to adjust the relative position of the light passing area of the slit 141 relative to the area illuminated by the light irradiated on the active shaping device 14. For example, referring to Figure 4 While keeping the width L and length h of the light-transmitting area of the slit 141 unchanged, increasing the distance d between the center of the light-transmitting area and the center of the active shaping device 14 can increase the oblique illumination angle, thereby improving the imaging resolution and the three-dimensional effect when imaging transparent samples within a certain range.
[0039] Optionally, the aperture of the slit 141 is adjustable. For example, in the embodiment of the utility model, a plurality of active shaping devices 14 with slits 141 of different lengths and widths can be prepared in advance. According to different types of samples and imaging requirements, slits 141 of different sizes can be used in a replacement manner to adjust the oblique illumination angle, further expanding the dynamic control range of imaging contrast and resolution.
[0040] Optionally, a polarizer 15 is provided between the light source 11 and the active shaping device 14, and an analyzer 16 matching the polarizer 15 is provided at the slit 141 of the active shaping device 14. Figures 4 to 6 In another possible implementation, polarization modulation of the light beam can be achieved by arranging a polarizer 15 between the light source 11 and the active shaping device 14, and arranging an analyzer 16 at the slit 141 of the active shaping device 14. For example, a light-transmitting position between the light source 11 and the active shaping device 14 located at the first working position, and a corresponding polarizer is arranged at the slit 141, so as to control the contrast and the overall luminous flux by modulating the polarization.
[0041] Exemplarily, the light-transmitting area of the slit 141 can be divided into an A area covered by the analyzer 16 and a B area through which light can pass. When the distance d between the center of the light-transmitting area and the center of the active shaping device 14 is a constant, the light flux of the A area is reduced by rotating the polarizer 15. During the rotation of the polarizer 15, the light transmittance of the A area will change linearly between 0% and 100%. The smaller the light flux of the A area, the higher the imaging contrast and the stronger the stereoscopic sense of the imaging, but at the same time, the imaging light flux will also be lower. The length h of the slit is generally determined according to the design requirements. The larger h is, the greater the light flux when the illumination spot reaches the sample surface.
[0042] Optionally, the active shaping device 14 is detachably connected to the illumination module 1; the objective lens modulation plate 24 is detachably connected to the imaging module 2. For example, in the embodiment of the utility model, the position adjustment of the active shaping device 14 and the objective lens modulation plate 24 between their respective working positions can be automatically controlled by setting a driving mechanism controlled by a host computer in the time difference incubator, or the active shaping device 14 and the objective lens modulation plate 24 can be disassembled and replaced to achieve mode switching according to actual lighting and imaging requirements by adopting a detachable connection. When not working, it is also convenient to store and maintain the active shaping device 14 and the objective lens modulation plate 24, the structure is simple, and the switching is convenient, which further improves the practicality and service life of the multimodal imaging system.
[0043] Optionally, the light source 11 is a light emitting diode light source 11, and the wavelength range of the light source 11 is 400 to 700 nm. Exemplarily, in the embodiment of the utility model, the light source 11 adopts an LED light source with a wavelength range of 400 to 700 nm, and the illumination method can adopt critical illumination or Kohler illumination. Kohler illumination has good illumination uniformity and is suitable for applications requiring high precision and high resolution, while critical illumination has a simple structure and is more suitable for inspecting sample surface quality and defects.
[0044] Optionally, the image collector 23 is a charge coupled device camera or a complementary metal oxide semiconductor camera. Exemplarily, in the embodiment of the utility model, the image collector 23 can use a CCD (Charge coupled Device) camera or a CMOS (Complementary Metal Oxide Semiconductor) camera as an imaging device.
[0045] The utility model also provides a time difference incubator, including: Figures 1 to 6 The multimodal imaging system shown.
[0046] Unless otherwise defined, the technical terms or scientific terms used herein shall have the usual meanings understood by persons with ordinary skills in the field to which the utility model belongs. The words "first", "second" and similar words used in the specification and claims of the utility model patent application do not indicate any order, quantity or importance, but are only used to distinguish different components. Similarly, words such as "one" or "one" do not indicate a quantity limitation, but indicate the existence of at least one. Words such as "include" or "comprise" and similar words mean that the elements or objects appearing before "include" or "comprise" cover the elements or objects listed after "include" or "comprise" and are extremely equivalent, and do not exclude other elements or objects. Words such as "connect" or "connected" and similar words are not limited to physical or mechanical connections, but may include electrical connections, whether direct or indirect. "Up", "down", "left", "right" and the like are only used to indicate relative positional relationships. When the absolute position of the described object changes, the relative positional relationship may also change accordingly.
[0047] The above description is only an optional embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention shall be included in the protection scope of the present invention.
Claims
1. A multimodal imaging system, characterized in that: include: An illumination module (1), an imaging module (2) and a culture dish (3), The lighting module (1) is fixedly arranged above the culture dish (3), and comprises a light source (11), a focusing system (12) and a movable shaping device (14); the light source (11) and the focusing system (12) are arranged at intervals in a direction close to the culture dish (3); a slit (141) is provided on the movable shaping device (14); the movable shaping device (14) is movably connected to the lighting module (1) so as to have a first working position located at the front focal plane of the focusing system (12), and a second working position adjusted away from the light path; The imaging module (2) is arranged below the culture dish (3), and comprises an objective lens (21), a lens barrel (22), an image collector (23), and an objective lens modulation plate (24); the objective lens (21), the lens barrel (22), and the image collector (23) are arranged at intervals in a direction away from the culture dish (3); the objective lens modulation plate (24) matches the slit (141); the objective lens modulation plate (24) is movably connected to the imaging module (2) so as to have a third working position located between the objective lens (21) and the lens barrel (22), and a fourth working position adjusted away from the optical path.
2. The multimodal imaging system according to claim 1, characterized in that: The movable shaping device (14) is configured to be capable of biaxial movement in the horizontal direction.
3. The multimodal imaging system according to claim 1, characterized in that: The light aperture of the slit (141) is adjustable.
4. The multimodal imaging system according to claim 3, characterized in that: A polarizer (15) is arranged between the light source (11) and the active shaping device (14), and an analyzer (16) matching the polarizer (15) is arranged at the slit (141) of the active shaping device (14).
5. The multimodal imaging system according to claim 1, characterized in that: The movable shaping device (14) is detachably connected to the illumination module (1); and the objective lens modulation plate (24) is detachably connected to the imaging module (2).
6. The multimodal imaging system according to any one of claims 1 to 5, characterized in that: The light source (11) is a light emitting diode light source (11), and the wavelength range of the light source (11) is 400 to 700 nm.
7. The multimodal imaging system according to any one of claims 1 to 5, characterized in that: The image collector (23) is a charge coupled device camera or a complementary metal oxide semiconductor camera.
8. A time difference incubator, characterized in that: Comprising a multimodal imaging system as described in any one of claims 1 to 5.