Biological enzyme fermentation, extraction and purification equipment

Abstract

The utility model relates to the technical field of extraction and purification, in particular to biological enzyme fermentation extraction and purification equipment which comprises a stirring box and a filtering box, an L-shaped connecting plate is fixedly connected to the bottom of the stirring box, one end, away from the stirring box, of the L-shaped connecting plate is fixedly connected to the side face of the filtering box, and a driving motor is installed on the L-shaped connecting plate. The output end of the driving motor is connected with one end of a movable shaft, the other end of the movable shaft penetrates into the stirring box, a plurality of stirring blades are fixedly connected to the movable shaft, the ends, away from the movable shaft, of the two transverse scraping plates are fixedly connected with the same vertical scraping plate, and a plurality of filtering openings are formed in the bottom of the filtering frame. A vibration assembly is arranged between the filter frame and the filter box, and a linkage assembly for driving the vibration assembly to work is arranged between the bottom of the stirring box and the top of the filter box, so that fermentation reactants are prevented from being stacked and adhered to the inner wall of the stirring box and the bottom of the cover plate, waste of material resources is avoided, and the speed of mixed liquid passing through the filter frame is increased; the filtering effect of the filtering frame is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of extraction and purification, in particular to a biological enzyme fermentation extraction and purification device. Background Art

[0002] Bioenzyme is a non-toxic, environmentally friendly biocatalyst, and its chemical nature is protein. Bioenzymes can be generally divided into six categories according to the nature of the reactions they catalyze, namely, oxidoreductases, transferases, hydrolases, lyases, isomerases, and synthetases. The extraction and purification of bioenzymes refers to the process of treating the enzyme-containing raw materials after cell crushing with appropriate solvents under certain conditions to fully dissolve the enzymes into the extract.

[0003] Patent document CN214612444U discloses a glucose oxidase fermentation extraction and purification device, "including a filter box, a mounting platform is fixed to one side wall of the filter box, a stirring box is fixed to the top of the mounting platform, the bottom of the stirring box is connected to the top of the filter box by a guide pipe, and the top of the stirring box is connected to a feed hopper; in the utility model, a stirring rod is driven by a driving motor to mix and stir the glucose oxidase and the diluent, so that the glucose oxidase can be mixed and fermented better, and the filter plate is driven by a moving component to reciprocate to filter and separate the glucose oxidase, which has a good purification effect, and the purified glucose oxidase is pushed by the push plate Liquid is discharged to prevent glucose oxidase liquid precipitation and accumulation at the bottom of the filter box, while improving the discharge efficiency. "In the process of mixing the glucose oxidase raw material and the diluent in the stirring box in the equipment, the fermentation reactants of glucose oxidase may accumulate on the inner wall of the stirring box, which is not conducive to cleaning and wastes material resources. In addition, the filter plate is in a horizontal moving state, and the filtering effect on the mixed liquid is limited. A small amount of mixed liquid precipitation may block the filter plate, reducing the filtering effect of the filter plate and causing the equipment to reduce the purification efficiency of glucose oxidase. For this reason, we propose a bio-enzyme fermentation extraction and purification equipment to solve the above problems. Utility Model Content

[0004] The purpose of the utility model is to provide a biological enzyme fermentation extraction and purification device to solve the problems raised in the above background technology.

[0005] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a biological enzyme fermentation extraction and purification equipment, including a stirring box and a filter box, the bottom of the stirring box is fixedly connected to an L-shaped connecting plate, one end of the L-shaped connecting plate away from the stirring box is fixedly connected to the side of the filter box, the bottom side of the stirring box and the top of the filter box are connected by a drainage pipe, a valve is arranged inside the drainage pipe, the top of the stirring box is connected to a cover plate, the cover plate is connected to a feed hopper, a driving motor is installed on the L-shaped connecting plate, the output end of the driving motor is connected to one end of a movable shaft, and the other end of the movable shaft passes through the inside of the stirring box. The stirring box is rotatably connected to the movable shaft, and a plurality of stirring blades are fixedly connected to the movable shaft. The movable shaft is fixedly connected with horizontal scrapers at positions near the bottom of the cover plate and the bottom of the inner wall of the stirring box. The ends of the two horizontal scrapers away from the movable shaft are fixedly connected with the same vertical scraper. A filter frame is arranged in the filter box, and a plurality of filter ports are opened at the bottom of the filter frame. A vibration assembly is arranged between the filter frame and the filter box, and a linkage assembly for driving the vibration assembly to work is arranged between the bottom of the stirring box and the top of the filter box. The bottom of the filter box is connected with a liquid outlet pipe, and a valve 2 is arranged inside the liquid outlet pipe.

[0006] Preferably, a plurality of support rods are fixedly connected to the bottom of the mixing box, and the bottoms of the plurality of support rods are flush with the bottom of the filter box.

[0007] Preferably, a handle is fixedly connected to the cover plate, and the cover plate is threadedly connected to the mixing box.

[0008] Preferably, a groove matching the movable shaft is formed at the bottom of the cover plate.

[0009] Preferably, the vibration assembly includes a vertical rod, a protrusion block and a spring, a plurality of springs are fixedly connected between the side of the filter frame and the inner wall of the filter box, a vertical rod is rotatably connected to the top of the inner wall of the filter frame, the surface of the vertical rod is attached to the inner wall of the filter frame, a plurality of protrusion blocks are rotatably connected to the vertical rod, and the protrusion blocks are circular.

[0010] Preferably, the linkage assembly includes rotating shaft one, rotating shaft two and a synchronous belt, the output end of the driving motor is fixedly connected to rotating shaft one, rotating shaft one is fixedly connected to the movable shaft, the vertical rod passes through the top of the filter box and is fixedly connected to rotating shaft two, and a synchronous belt is connected between rotating shaft one and rotating shaft two.

[0011] Preferably, an inclined plate is fixedly connected to the inner wall of the filter box at a position close to the liquid outlet pipe.

[0012] Compared with the prior art, the beneficial effects of the utility model are as follows: the coordinated use of the driving motor, the movable shaft, the stirring blades, the horizontal scraper and the vertical scraper can stir and mix the glucose oxidase raw material and the diluent, and scrape the inner wall of the stirring box and the bottom of the cover plate to prevent the fermentation reactants from accumulating and adhering to the inner wall of the stirring box and the bottom of the cover plate, thereby avoiding waste of material resources; the detachable design of the cover plate and the stirring box facilitates the inspection and maintenance of the structural components inside the stirring box; the setting of the vibration component speeds up the speed at which the mixed liquid passes through the filter frame, prevents the residual mixed liquid in the filter frame from clogging the filter port, and improves the filtering effect of the filter frame; the setting of the linkage component can simultaneously perform mixed fermentation and filtration purification on the glucose oxidase through the same power source, thereby increasing the convenience of equipment use. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 This is a schematic diagram of the structure of the bio-enzyme fermentation extraction and purification equipment of the utility model;

[0014] Figure 2 This is a cross-sectional view of the bio-enzyme fermentation extraction and purification equipment;

[0015] Figure 3 for Figure 2 Enlarged view of point A in the middle;

[0016] Figure 4 Set up the schematic for the groove.

[0017] In the figure: 1. mixing box; 2. filter box; 3. L-shaped connecting plate; 4. drainage pipe; 5. valve one; 6. cover plate; 7. feed hopper; 8. drive motor; 9. movable shaft; 10. mixing blade; 11. horizontal scraper; 12. vertical scraper; 13. filter frame; 14. filter port; 15. liquid outlet pipe; 16. valve two; 17. support rod; 18. handle; 19. groove; 20. vertical rod; 21. raised block; 22. spring; 23. rotating shaft one; 24. rotating shaft two; 25. synchronous belt; 26. tilting plate. DETAILED DESCRIPTION

[0018] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0019] See also Figure 1-4The utility model provides a technical solution: a biological enzyme fermentation extraction and purification equipment, including a stirring box 1 and a filtering box 2, the bottom of the stirring box 1 is fixedly connected with an L-shaped connecting plate 3, the end of the L-shaped connecting plate 3 away from the stirring box 1 is fixedly connected to the side of the filtering box 2, the bottom side of the stirring box 1 and the top of the filtering box 2 are connected with a drainage pipe 4, a valve 5 is arranged inside the drainage pipe 4, the top of the stirring box 1 is connected with a cover plate 6, the cover plate 6 is connected with a feed hopper 7, a driving motor 8 is installed on the L-shaped connecting plate 3, the output end of the driving motor 8 is connected with one end of a movable shaft 9, the other end of the movable shaft 9 passes through the inside of the stirring box 1, the stirring box 1 is rotatably connected to the movable shaft 9, a plurality of stirring blades 10 are fixedly connected to the movable shaft 9, and the movable shaft 9 is close to the bottom of the cover plate 6. A horizontal scraper 11 is fixedly connected to the bottom of the inner wall of the mixing box 1, and one end of the two horizontal scrapers 11 away from the movable shaft 9 is fixedly connected to the same vertical scraper 12. A filter frame 13 is arranged in the filter box 2, and a plurality of filter ports 14 are provided at the bottom of the filter frame 13. A vibration component is arranged between the filter frame 13 and the filter box 2. A linkage component for driving the vibration component is arranged between the bottom of the mixing box 1 and the top of the filter box 2. A liquid outlet pipe 15 is connected to the bottom of the filter box 2, and a valve 16 is arranged inside the liquid outlet pipe 15. When it is necessary to filter and purify the glucose oxidase, the glucose oxidase raw material and the diluent are poured into the mixing box 1 through the feed hopper 7, and the drive motor 8 is started to drive the movable shaft 9 to rotate, and the rotation of the movable shaft 9 drives a plurality of stirring blades. 10 rotates to stir and mix the glucose oxidase raw material and the diluent. During the rotation of the movable shaft 9, the horizontal scraper 11 and the vertical scraper 12 are driven to scrape the inner wall of the mixing box 1 and the bottom of the cover plate 6 respectively to prevent the fermentation reactants from accumulating and adhering to the inner wall of the mixing box 1 and the bottom of the cover plate 6, thereby avoiding waste of material resources. When the glucose oxidase raw material and the diluent are completely fused and reacted, the valve 15 is opened. After the valve 15 is opened, the mixed liquid enters the filter frame 13 in the filter box 2 through the drainage pipe 4, and is filtered and separated through several filter ports 14 at the bottom of the filter frame 13, thereby achieving the fermentation purification effect. When the driving motor 8 is started, the rotating shaft 1 23 is driven to rotate. The rotation of the rotating shaft 1 23 drives the synchronous belt 25 to transmit, so that the rotating shaft 24 is synchronously The filter frame 13 is provided with a filter element 14 and a plurality of filter elements 16 are provided to filter the filter element 13. The filter element 13 is provided with a filter element 14 and a plurality of filter elements 16 are provided to filter the filter element 13. The filter element 13 is provided with a filter element 14 and a plurality of filter elements 16 are provided to filter the filter element 13. The filter element 13 is provided with a filter element 14 and a plurality of filter elements 16 are provided to filter the filter element 13. The filter element 13 is provided with a filter element 14 and a plurality of filter elements 16 are provided to filter the filter element 13. The filter element 13 is provided with a filter element 14 and a plurality of filter elements 16 are provided to filter the filter element 13. The filter element 13 is provided with a filter element 14 and a plurality of filter elements 16 are provided to filter the filter element 13. The filter element 13 is provided with a filter element 14 and a plurality of filter elements 16 are provided to filter the filter element 13. The filter element 13 is provided with a filter element 14 and a plurality of filter elements 16 are provided to filter the filter element 13.Those skilled in the art can fully realize it, and no further explanation is needed.

[0020] Furthermore, a plurality of support rods 17 are fixedly connected to the bottom of the mixing box 1 , and the bottoms of the plurality of support rods 17 are flush with the bottom of the filter box 2 . The support rods 17 are arranged to provide support for the mixing box 1 and maintain the placement stability of the mixing box 1 .

[0021] Furthermore, a handle 18 is fixedly connected to the cover plate 6, and the cover plate 6 is threadedly connected to the mixing box 1. The handle 18 facilitates quick disassembly and assembly of the cover plate 6 on the mixing box 1. The detachable design of the cover plate 6 and the mixing box 1 facilitates inspection and maintenance of the structural components inside the mixing box 1.

[0022] Furthermore, a groove 19 adapted to the movable shaft 9 is provided at the bottom of the cover plate 6 , and when the cover plate 6 is threadedly connected to the mixing box 1 , the top of the movable shaft 9 is embedded in the groove 19 , thereby increasing the connectivity between the movable shaft 9 and the cover plate 6 .

[0023] Furthermore, the vibration assembly includes a vertical rod 20, a protruding block 21 and a spring 22. A plurality of springs 22 are fixedly connected between the side of the filter frame 13 and the inner wall of the filter box 2. The top of the inner wall of the filter frame 13 is rotatably connected to the vertical rod 20. The surface of the vertical rod 20 is attached to the inner wall of the filter frame 13. A plurality of protruding blocks 21 are rotatably connected to the vertical rod 20. The protruding blocks 21 are circular. During the rotation of the vertical rod 20, the protruding blocks 21 are driven to continuously contact the inner wall of the filter frame 13, causing a part of the spring 22 to contract and another part of the spring 22 to be stretched. When the protruding blocks 21 are away from the inner wall of the filter frame 13, the spring 22 automatically resets and drives the filter frame 13 to shake, thereby preventing the residual mixed liquid from clogging the filter port 14 and improving the filtering effect of the filter frame 13.

[0024] Furthermore, the linkage assembly includes a rotating shaft 23, a rotating shaft 24 and a synchronous belt 25. The output end of the driving motor 8 is fixedly connected to the rotating shaft 23, the rotating shaft 23 is fixedly connected to the movable shaft 9, the vertical rod 20 passes through the top of the filter box 2 and is fixedly connected to the rotating shaft 24, and the synchronous belt 25 is transmission-connected between the rotating shaft 23 and the rotating shaft 24. The coordinated use of the rotating shaft 23, the rotating shaft 24 and the synchronous belt 25 can simultaneously carry out mixed fermentation and filtration purification of glucose oxidase through the same power source, thereby increasing the convenience of equipment use.

[0025] Furthermore, an inclined plate 26 is fixedly connected to the inner wall of the filter box 2 near the liquid outlet pipe 15 to accelerate the flow rate of the purified glucose oxidase liquid.

[0026] Working principle: When in use, the glucose oxidase raw material and the diluent are poured into the mixing box 1 through the feed hopper 7, and the driving motor 8 is started to drive the movable shaft 9 to rotate. The rotation of the movable shaft 9 drives a plurality of stirring blades 10 to rotate, so as to mix the glucose oxidase raw material and the diluent. During the rotation of the movable shaft 9, the horizontal scraper 11 and the vertical scraper 12 are driven to scrape the inner wall of the mixing box 1 and the bottom of the cover plate 6 respectively to prevent the fermentation reactants from accumulating and adhering to the inner wall of the mixing box 1 and the bottom of the cover plate 6, thereby avoiding waste of material resources. When the glucose oxidase raw material and the diluent are completely fused and reacted, the valve 5 is opened. After the valve 5 is opened, the mixed liquid enters the filter frame 13 in the filter box 2 through the drainage tube 4, and is filtered and separated through a plurality of filter ports 14 at the bottom of the filter frame 13. , thereby achieving the fermentation and purification effect, the driving motor 8 starts and drives the rotating shaft 1 23 to rotate, and the rotation of the rotating shaft 1 23 drives the synchronous belt 25 to transmit, so that the rotating shaft 24 rotates synchronously, and then drives the vertical rod 20 to rotate, and the rotation of the vertical rod 20 causes the protruding block 21 to rotate and hit the filter frame 13, and the spring 22 is forced to drive the filter frame 13 to vibrate, thereby accelerating the speed of the mixed liquid passing through the filter frame 13, preventing the residual mixed liquid in the filter frame 13 from clogging the filter port 14, and improving the filtering effect of the filter frame 13. The structure is simple and the linkage is strong. The mixed liquid flows into the inclined plate 26 through the filter frame 13, and one side of the inclined plate 26 is inclined toward the liquid outlet pipe 15. The valve 2 16 is opened, which is convenient for quickly discharging the purified glucose oxidase liquid through the liquid outlet pipe 15 to complete the fermentation and purification of glucose oxidase.

[0027] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A biological enzyme fermentation extraction and purification device, comprising a stirring box (1) and a filtering box (2), characterized in that: The bottom of the mixing box (1) is fixedly connected to an L-shaped connecting plate (3), one end of the L-shaped connecting plate (3) away from the mixing box (1) is fixedly connected to the side of the filter box (2), the bottom side of the mixing box (1) and the top of the filter box (2) are connected to a drainage pipe (4), a valve (5) is arranged inside the drainage pipe (4), the top of the mixing box (1) is connected to a cover plate (6), the cover plate (6) is connected to a feed hopper (7), a driving motor (8) is installed on the L-shaped connecting plate (3), the output end of the driving motor (8) is connected to one end of a movable shaft (9), the other end of the movable shaft (9) passes through the inside of the mixing box (1), the mixing box (1) is rotatably connected to the movable shaft (9), and the movable shaft (9) is fixedly connected to the inner wall of the mixing box (1). A plurality of stirring blades (10) are connected, and the movable shaft (9) is fixedly connected to a transverse scraper (11) at a position close to the bottom of the cover plate (6) and the bottom of the inner wall of the stirring box (1), and the ends of the two transverse scrapers (11) away from the movable shaft (9) are fixedly connected to the same vertical scraper (12), a filter frame (13) is arranged in the filter box (2), and a plurality of filter ports (14) are opened at the bottom of the filter frame (13), a vibration component is arranged between the filter frame (13) and the filter box (2), and a linkage component for driving the vibration component to work is arranged between the bottom of the stirring box (1) and the top of the filter box (2), and the bottom of the filter box (2) is connected to a liquid outlet pipe (15), and a valve 2 (16) is arranged inside the liquid outlet pipe (15).

2. A biological enzyme fermentation extraction and purification equipment according to claim 1, characterized in that: A plurality of support rods (17) are fixedly connected to the bottom of the mixing box (1), and the bottoms of the plurality of support rods (17) are flush with the bottom of the filter box (2).

3. The bio-enzyme fermentation extraction and purification equipment according to claim 1, characterized in that: A handle (18) is fixedly connected to the cover plate (6), and the cover plate (6) is threadedly connected to the mixing box (1).

4. A biological enzyme fermentation extraction and purification equipment according to claim 3, characterized in that: The bottom of the cover plate (6) is provided with a groove (19) adapted to the movable shaft (9).

5. The bio-enzyme fermentation extraction and purification equipment according to claim 1, characterized in that: The vibration assembly comprises a vertical rod (20), a protruding block (21) and a spring (22); a plurality of springs (22) are fixedly connected between the side of the filter frame (13) and the inner wall of the filter box (2); a vertical rod (20) is rotatably connected to the top of the inner wall of the filter frame (13); a surface of the vertical rod (20) is attached to the inner wall of the filter frame (13); a plurality of protruding blocks (21) are rotatably connected to the vertical rod (20); and the protruding blocks (21) are circular.

6. The bio-enzyme fermentation extraction and purification equipment according to claim 5, characterized in that: The linkage assembly comprises a rotating shaft 1 (23), a rotating shaft 2 (24) and a synchronous belt (25); the output end of the driving motor (8) is fixedly connected to the rotating shaft 1 (23); the rotating shaft 1 (23) is fixedly connected to the movable shaft (9); the vertical rod (20) passes through the top of the filter box (2) and is fixedly connected to the rotating shaft 2 (24); and a synchronous belt (25) is transmission-connected between the rotating shaft 1 (23) and the rotating shaft 2 (24).

7. The bio-enzyme fermentation extraction and purification equipment according to claim 1, characterized in that: An inclined plate (26) is fixedly connected to the inner wall of the filter box (2) at a position close to the liquid outlet pipe (15).

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