Parallel bioreactor gas path system

Abstract

The utility model discloses a parallel bioreactor gas path system. The parallel bioreactor gas path system comprises a gas path logic valve group and a gas path logic control panel, wherein a plurality of independent gas inlet channels and a plurality of independent tank gas channels are arranged in the gas path logic control panel, and each gas inlet channel is respectively communicated with the plurality of tank gas channels through a plurality of connecting channels; the switch electromagnetic valve group is arranged on the gas circuit logic valve plate, the switch electromagnetic valve group comprises a plurality of switch electromagnetic valves which work independently, and each switch electromagnetic valve is arranged on a connecting channel which is communicated with one gas inlet channel and one tank gas channel and is used for opening and closing the connecting channel; the flow controller group comprises a plurality of flow controllers working independently, and each flow controller is connected with the gas outlet end of the gas channel of the tank body; and the culture tank group comprises a plurality of culture tanks, and each culture tank is connected with one flow controller. The parallel bioreactor gas path system provided by the utility model is simpler and more compact in structure, and the cost is greatly reduced.

Description

Technical Field

[0001] The utility model particularly relates to a parallel bioreactor gas path system, belonging to the technical field of cell and microorganism cultivation in the process of food and medicine production and research and development. Background Art

[0002] The rapid production of the above-mentioned protein drugs, antibodies, and vaccine products all rely on equipment that can truly realize large-scale cultivation of animal cells - bioreactors. Bioreactors are the bridge between laboratory technical research and factory-scale production of vaccines and protein drugs. High-throughput screening is one of the important technical means in the field of life sciences and drug innovation. Its core is to obtain a large amount of information through a single experiment and find valuable information from it. At the same time, stable and highly expressed cell clones / cell lines and their optimized culture strategies, culture conditions, and culture processes are also the key to the efficient and low-cost production of biopharmaceuticals. The screening of microbial strains, the optimization of culture media, and the optimization of processes are inseparable from experimental bioreactors, which are used to simulate various process parameters and environmental conditions on industrial bioreactors.

[0003] The ventilation method is one of the key technologies in the design and process scale-up of the cell bioreactor culture process, which has an important impact on the amount of metabolites and cell damage. A good ventilation method should be effective and gentle. By introducing different gases, usually air, oxygen, nitrogen and carbon dioxide. The environment inside the bioreactor, such as the dissolved oxygen level and pH balance, is adjusted by the introduction ratio of different gases. The existing parallel biological fermentation system is constrained by physical factors such as hardware scale and size. If more tanks are required, it will seriously occupy laboratory space resources.

[0004] According to the traditional reaction tank ventilation scheme, each tank requires 4 gas flow controllers. If 8 tanks or more tanks are used, 32 or more flow controllers are required. In an 8-tank small bioreactor device, 32 flow controllers will greatly occupy the equipment volume, and the gas piping is also quite complicated. Utility Model Content

[0005] The main purpose of the utility model is to provide a parallel bioreactor gas path system, which adopts a multi-channel pipeline switching design, and a culture tube is equipped with a total flow controller. The overall gas supply ratio of the gas is controlled by the ventilation time ratio of different gases in a specific time period, and the ventilation volume within the ventilation ratio time is controlled by the flow controller. The precise ventilation control of gases with different flow rates is achieved through the combined control of the switching solenoid valve and the flow controller. The combination of the switching solenoid valve and the flow controller replaces the original multiple flow controllers to control ventilation, and the structure is more compact and the equipment cost is lower, thereby overcoming the shortcomings of the prior art.

[0006] In order to achieve the above-mentioned utility model purpose, the technical solution adopted by the utility model includes:

[0007] The utility model provides a parallel bioreactor gas path system, comprising:

[0008] A gas circuit logic valve plate, wherein the gas circuit logic control plate has a plurality of independent air inlet channels and a plurality of independent tank gas channels inside, and each of the air inlet channels is connected to the plurality of tank gas channels via a plurality of connecting channels;

[0009] A switch solenoid valve group is arranged on the gas path logic valve plate, and the switch solenoid valve group includes a plurality of independently working switch solenoid valves, each of which is arranged on a connecting channel connecting an air inlet channel and a tank gas channel, and is used to open and close the connecting channel;

[0010] A flow controller group, comprising a plurality of independently working flow controllers, each of which is connected to a gas outlet end of the tank gas channel;

[0011] A culture tank group, comprising a plurality of culture tanks, each of which is connected to a flow controller;

[0012] Each of the air inlet channels is connected to a culture tank via a switch electromagnetic valve, a tank gas channel, and a flow controller to form a gas passage.

[0013] Further, the air inlet channel and / or the tube body gas channel include a hole arranged inside the gas circuit logic valve plate, or the air inlet channel and / or the tube body gas channel include a gas pipeline arranged inside the gas circuit logic valve plate.

[0014] Furthermore, the plurality of air inlet passages are arranged in parallel, and the plurality of tank gas passages are arranged in parallel.

[0015] Furthermore, the plurality of air inlet channels and the plurality of tank body gas channels are arranged in an alternating manner; and / or the plurality of air inlet channels and the plurality of tank body gas channels are arranged in an alternating manner vertically.

[0016] In a more specific embodiment, the parallel bioreactor gas circuit system also includes: multiple pressure gauges, which are arranged on the gas circuit logic valve plate, each of the pressure gauges is arranged at the inlet section of the air inlet channel and is used to monitor the pressure of the gas entering the air inlet channel.

[0017] In a more specific embodiment, the parallel bioreactor gas path system also includes: multiple air intake sources, each of the air intake sources is connected to an air intake channel, each of the culture tanks is connected to the multiple air intake sources via a flow controller, and, in the same time period, only one of the multiple gas passages between a culture tank and the multiple air intake sources is in a passage state.

[0018] Furthermore, the air intake source has a pressure switch, which is used to monitor the pressure of the gas output by the air intake source and output an alarm signal when the monitored pressure value exceeds a set threshold.

[0019] In a more specific embodiment, the parallel bioreactor gas path system also includes: a plurality of gas buffer tanks, each of which is arranged between the flow controller and the culture tank, and is respectively connected to the flow controller and the culture tank, and a gas passage for one-way gas flow is formed between each of the air inlet channels, a switch solenoid valve, a tank gas channel, a flow controller, and a culture tank.

[0020] Compared with the prior art, the advantages of the utility model include:

[0021] The utility model provides a parallel bioreactor gas circuit system, which adopts a multi-channel pipeline switching design. A culture tank is equipped with a total flow controller, which can realize the ventilation time ratio of different gases in a specific time period to control the overall gas supply ratio of the gas. At the same time, the ventilation volume within the ventilation ratio time is controlled by the flow controller, and the precise ventilation control of gases with different flow rates is achieved through the combination of the gas switch valve and the flow controller.

[0022] The utility model provides a parallel bioreactor gas path system, which adopts an integrated gas path logic valve plate control, making the structure more compact, reducing joint connection points and leakage points, and realizing precise control of multi-path gases of multiple culture tanks through the combined control of the switch solenoid valve and the flow controller on the gas path logic valve plate.

[0023] The utility model provides a parallel bioreactor gas path system, which has a compact structure, a simple control system and a greatly reduced cost compared to using 32 flow controllers;

[0024] The utility model provides a parallel bioreactor gas path system, which can realize accurate gas introduction through a one-way open valve plate and a buffer tank, and avoid environmental fluctuations in the culture tank caused by time-sharing gas supply of different gases in a circulation period. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0026] Figure 1 It is a schematic structure of a gas circuit system of a parallel bioreactor and its ventilation principle diagram provided in a typical implementation case of the utility model;

[0027] Figure 2 It is a structural schematic diagram of a gas path system of a parallel bioreactor provided in a typical implementation case of the utility model;

[0028] Figure 3 It is a top view of a parallel bioreactor gas path system provided in a typical implementation case of the utility model. DETAILED DESCRIPTION

[0029] In view of the deficiencies in the prior art, the inventor of this case has proposed the technical solution of the utility model after long-term research and extensive practice. The technical solution, its implementation process and principle, etc. will be further explained in conjunction with the accompanying drawings and specific implementation cases. Unless otherwise specified, the pressure switch, pressure gauge, switch solenoid valve, flow controller, pipeline joint, etc. used in the embodiment of the utility model are all known to those skilled in the art, and they can all be purchased commercially. Their own structures are irrelevant to the inventive concept of this case, and their specific structures and product models are not limited and explained here.

[0030] Example 1

[0031] See also Figure 1 A parallel bioreactor gas path system comprises: four groups of intake gas sources A / B / C / D with pressure switches, four groups of pressure gauges 160, eight groups of culture tanks A1 / A2 / A3 / A4 / A5 / A6 / A7 / A8, four independently arranged intake channels 110, eight independently arranged tank gas channels 120, eight groups of flow controllers 140 and thirty-two groups of switch solenoid valves 130, each intake gas source is connected to an intake channel 110 via a pressure gauge 160, each intake channel 110 is also independently connected to eight tank gas channels 120 via eight groups of switch solenoid valves 130, each tank gas channel 120 is also independently connected to four groups of intake gas sources A / B / C / D via four groups of switch solenoid valves 130, and each tank gas channel 120 is also connected to a culture tank via a flow controller 140.

[0032] It should be noted that any culture tank A1 / A2 / A3 / A4 / A5 / A6 / A7 / A8 can be connected to four groups of air intake sources A / B / C / D, and any air intake source A / B / C / D is connected to a culture tank A1 / A2 / A3 / A4 / A5 / A6 / A7 / A8 in sequence through a pressure gauge 160, an air intake channel 110, a switch solenoid valve 130 and a tank gas channel 120, a flow controller 140, and a gas passage for unidirectional and quantitative flow of gas is formed between any air intake source A / B / C / D and any culture tank A1 / A2 / A3 / A4 / A5 / A6 / A7 / A8. Figure 1 The parallel bioreactor gas path system shown includes thirty-two gas paths. It should be noted that the required gas ratio in the culture tank is realized by controlling the on-off time ratio cycle of multiple solenoid valves on multiple gas paths corresponding to the culture tank, so as to realize the proportional gas supply, and the four switch solenoid valves on the four gas paths connected to the same culture tank only have one gas conduction in the same cycle time, and synchronously, the flow controller measures and controls according to the gas type, and at the same time, in the ventilation control strategy, the circulation ventilation of the four gases is continuously and uninterruptedly supplied to ensure the constant pressure of the gas in the culture tank.

[0033] In this embodiment, the air intake source, the four sets of pressure gauges 160, the air intake channel 110, the tank gas channel 120, the flow controller 140, and the culture tank can be connected via connecting pipes and pipe joints.

[0034] It should also be noted that Figure 1 The four groups of air inlet sources A / B / C / D with pressure switches and eight groups of culture tanks A1 / A2 / A3 / A4 / A5 / A6 / A7 / A8 shown are merely exemplary. The parallel bioreactor gas circuit system provided by the present invention can of course also include any other number of air inlet sources and culture tanks.

[0035] In this embodiment, four air intake channels 110 and eight tank gas channels 120 are vertically staggered, and each air intake channel 110 is independently and controllably connected to the eight tank gas channels 120 via eight groups of switch solenoid valves. Specifically, each air intake channel 110 is connected to a tank gas channel 120 via a connecting channel. The switch solenoid valve 130 is arranged on the connecting channel and is used to open and close the connecting channel, thereby controlling the connection state between the air intake channel 110 and the tank gas channel 120 (i.e., connected or not connected).

[0036] In this embodiment, please refer to Figure 2 and Figure 3The parallel bioreactor gas path system includes a gas path logic valve plate 170, four air inlet channels 110 and eight tank gas channels 120 are arranged inside the gas path logic control board, four groups of pressure gauges 160 and thirty-two groups of electromagnetic switch valves are correspondingly arranged on the gas path logic valve plate 170, each of the pressure gauges 160 is correspondingly arranged at the entrance section of the air inlet channel 110, and is used to monitor the pressure of the gas entering the air inlet channel 110. When the pressure gauge 160 detects that the pressure of the gas entering the air inlet channel 110 from the air inlet source is too low or too high, the pressure alarm is output, and manual inspection and intervention are performed. The pressure of the output gas can be monitored by adjusting the pressure switch of the air inlet source and an alarm signal can be output when the pressure of the output gas exceeds the set threshold. The alarm signal can be at least one of an acoustic signal and a light signal.

[0037] In this embodiment, the air intake channel 110 and / or the tube body gas channel may be integrally arranged with the gas circuit logic valve plate 170, that is, the air intake channel 110 and / or the tube body gas channel may be holes arranged inside the gas circuit logic valve plate 170, or the air intake channel 110 and / or the tube body gas channel may be gas pipelines arranged inside the gas circuit logic valve plate 170.

[0038] In this embodiment, when the ratio of A gas m%, B gas n%, C gas p%, and D gas k% is required in the A1 culture tank, the ratio of the on-off time of the four switch solenoid valves 130 located in the same gas passage as the A1 culture tank is controlled to be m:n:p:k, and the cycle is on and off within a certain time period to achieve the proportional supply of A gas, B gas, C gas, and D gas. It can be understood that within the same time period, only one of the four switch solenoid valves 130 corresponding to the A1 culture tank remains in an open state, and the quantitative supply of the four gases can be achieved only through one flow controller 140. In other words, the four switch solenoid valves 130 only allow one gas to be conducted within the same cycle time, and the flow controller measures and controls according to the type of gas synchronously. At the same time, in terms of ventilation control strategy, the continuous and uninterrupted supply of the four gases through the cyclic ventilation ensures the constant pressure of the gas in the culture tank.

[0039] In this embodiment, a plurality of connecting pipelines are further provided between the gas circuit logic valve plate 170 and the culture tanks A1 / A2 / A3 / A4 / A5 / A6 / A7 / A8, and each of the tank gas channels 120 is connected to a culture tank A1 / A2 / A3 / A4 / A5 / A6 / A7 / A8 via a connecting pipeline, wherein the connecting pipeline includes a first pipeline section and a second pipeline section connected in sequence, the first pipeline section is directly connected to the tank gas channel 120, and the second pipeline section is directly connected to the culture tanks A1 / A2 / A3 / A4 / A5 / A6 / A7 / A8. 4 / A5 / A6 / A7 / A8 are connected, the flow controller 140 is arranged in the first pipeline section, and the second pipeline section is also provided with a valve plate which can only allow the gas to pass from the first pipeline section to the second pipeline section in one direction, and the valve plate is configured to be able to move only in the direction tending away from the first pipeline section to open the connecting pipeline in one direction, and the flow controller 140 is arranged on the side of the valve plate close to the tank gas channel 120 to avoid the backflow of the gas passing through the flow controller 140 in the forward direction, thereby causing the problem of inaccurate volume of the subsequent airflow.

[0040] Example 2

[0041] See also Figure 1 The structure of a parallel bioreactor gas path system in this embodiment is basically the same as that in Embodiment 1 or Embodiment 2, and the same parts of the two are not described here. The difference between the two is that:

[0042] In this embodiment, in order to avoid environmental fluctuations in the culture tank caused by the time-sharing supply of different gases during the circulation period, a buffer tank 150 is further arranged between the flow controller 140 and the culture tube. The gas first enters the buffer tank 150 through the flow controller 140 and then enters the culture tube to fully mix the gas, so as to minimize the fluctuation of the gas composition entering the culture tank and make the gas release more gentle. Specifically, the valve plate is arranged in the connecting pipeline between the flow controller 140 and the buffer tank 150.

[0043] It should be understood that the above embodiments are only for illustrating the technical concept and features of the utility model, and their purpose is to enable people familiar with the technology to understand the content of the utility model and implement it accordingly, and they cannot be used to limit the protection scope of the utility model. Any equivalent changes or modifications made according to the spirit of the utility model should be included in the protection scope of the utility model.

Claims

1. A parallel bioreactor gas path system, characterized in that: include: A gas circuit logic valve plate, wherein the gas circuit logic valve plate has a plurality of independent air inlet channels and a plurality of independent tank gas channels inside, and each of the air inlet channels is connected to the plurality of tank gas channels via a plurality of connecting channels; A switch solenoid valve group is arranged on the gas path logic valve plate, and the switch solenoid valve group includes a plurality of independently working switch solenoid valves, each of which is arranged on a connecting channel connecting an air inlet channel and a tank gas channel, and is used to open and close the connecting channel; A flow controller group, comprising a plurality of independently working flow controllers, each of which is connected to a gas outlet end of the tank gas channel; The culture tank group includes a plurality of culture tanks, each of which is connected to a flow controller; each of the air inlet channels is connected to a culture tank via a switch solenoid valve, a tank gas channel, and a flow controller to form a gas passage.

2. The parallel bioreactor gas path system according to claim 1, characterized in that: The air inlet passage and / or the tank gas passage include a hole arranged inside the gas path logic valve plate.

3. The parallel bioreactor gas path system according to claim 1, characterized in that: The air inlet passage and / or the tank gas passage include a gas pipeline arranged inside the gas circuit logic valve plate.

4. The parallel bioreactor gas path system according to claim 1, 2 or 3, characterized in that: The plurality of air inlet passages are arranged in parallel, and the plurality of tank gas passages are arranged in parallel.

5. The parallel bioreactor gas path system according to claim 4, characterized in that: The plurality of air inlet passages and the plurality of tank gas passages are arranged alternately.

6. The parallel bioreactor gas path system according to claim 5, characterized in that: The plurality of air inlet passages and the plurality of tank body gas passages are vertically staggered.

7. The parallel bioreactor gas path system according to claim 1, characterized in that: Also includes: A plurality of pressure gauges are arranged on the gas path logic valve plate, each of the pressure gauges is arranged correspondingly at the inlet section of the intake passage and is used to monitor the pressure of the gas entering the intake passage.

8. The parallel bioreactor gas path system according to claim 7, characterized in that: The parallel bioreactor gas path system also includes: multiple air intake sources, each of which is connected to an air intake channel, and each of the culture tanks is connected to the multiple air intake sources via a flow controller, and in the same time period, only one of the multiple gas passages between a culture tank and the multiple air intake sources is in a passage state.

9. The parallel bioreactor gas path system according to claim 8, characterized in that: The air intake source has a pressure switch, which is used to monitor the pressure of the gas output by the air intake source and output an alarm signal when the monitored pressure value exceeds a set threshold.

10. The parallel bioreactor gas path system according to claim 1, characterized in that: Also includes: Multiple gas buffer tanks, each of which is arranged between the flow controller and the culture tank, and is respectively connected to the flow controller and the culture tank, and a gas passage for one-way gas flow is formed between each of the air inlet channel, a switch solenoid valve, a tank gas channel, a flow controller, and a culture tank.

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