Cell culture device

Abstract

The utility model relates to the technical field of cell culture, in particular to a cell culture device which comprises a constant-temperature box and further comprises a conveying belt, a cover opening assembly, a universal ball, a liquid pumping suction nozzle and a liquid adding suction nozzle, and a culture dish access channel is arranged at the side end of the constant-temperature box in a penetrating mode in the horizontal direction; the conveyor belt is arranged inside the constant-temperature box, horizontally penetrates through the culture dish access channel and is used for intermittently conveying culture dishes; the uncovering assembly is arranged on one side of the conveying belt, fixedly arranged in the incubator and used for uncovering the culture dishes, and the uncovering direction is vertical; according to the cell culture device provided by the utility model, the liquid changing process does not need to be manually opened one by one, the operation is convenient and fast, the workload is small, meanwhile, the liquid changing process is carried out in a closed constant-temperature box, cells are effectively protected from being polluted and damaged, and the problems that an existing liquid changing mode is tedious in operation, large in workload and easy to pollute the cells are solved.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture, in particular to a cell culture device. Background Art

[0002] Cell culture refers to a method of simulating the internal environment in vitro to allow cells to grow and reproduce while maintaining their main structure and function. It is an important and commonly used technique in cell biology research methods. A large number of cells can be obtained through cell culture.

[0003] Laboratory cell culture is mainly carried out using culture dishes, especially during the process of cell expansion, when a large number of cell culture dishes are required.

[0004] During the cell culture process, the liquid needs to be changed regularly. The traditional method is to use manual liquid change. If there are many culture dishes that need to be changed, each dish needs to be opened manually one by one for operation each time, which is cumbersome and labor-intensive. In addition, during the liquid change process, the cells are exposed to the outside for a long time, which can easily cause cell contamination and damage. Utility Model Content

[0005] In view of this, the utility model proposes a cell culture device, the liquid replacement process does not require manual opening of the covers one by one, which is convenient and quick, and has a small workload. At the same time, the liquid replacement process is carried out in a closed constant temperature box, which effectively protects the cells from being contaminated and damaged, and solves the problems of the existing liquid replacement method that is cumbersome to operate, has a large workload, and is easy to contaminate cells.

[0006] The technical solution of the utility model is achieved in this way:

[0007] The utility model provides a cell culture device, which comprises a constant temperature box, a conveyor belt, a cover opening component, a universal ball, a liquid extraction nozzle and a liquid addition nozzle, wherein:

[0008] A culture dish access channel is provided through the side end of the incubator in a horizontal direction;

[0009] The conveyor belt is arranged inside the thermostatic box and horizontally passes through the culture dish access channel for intermittently conveying the culture dishes;

[0010] The cover opening assembly is arranged on one side of the conveyor belt and fixedly arranged inside the constant temperature box, and is used for opening the cover of the culture dish, and the cover opening direction is vertical;

[0011] Two universal balls are rotatably arranged on the top of the thermostatic box;

[0012] One end of the liquid suction nozzle passes through the center of one of the universal balls and extends to the interior of the constant temperature box, and the other end is used to connect to the suction pump;

[0013] One end of the liquid adding nozzle passes through the center of the other universal ball and extends to the interior of the constant temperature box, and the other end is used for connecting to the liquid adding pump.

[0014] On the basis of the above technical solution, preferably, the constant temperature box includes a box body, a partition, a consumable box and a T-shaped box cover, wherein:

[0015] Two partitions are fixedly arranged inside the box, and a first buffer zone, a second buffer zone and an operation zone are formed inside the box by the two partitions. The first buffer zone, the operation zone and the second buffer zone are arranged in sequence along the conveying direction of the conveyor belt;

[0016] The consumables box is arranged on the side of the operation area and fixedly arranged on the side of the box body, and the consumables box is communicated with the interior of the box body;

[0017] The T-shaped box cover is detachably arranged on the top of the box body and the consumable box;

[0018] The culture dish access channel horizontally passes through the box body and the lower end of the partition;

[0019] The cover opening assembly is arranged in the operation area;

[0020] The universal ball is rotatably arranged on the T-shaped box cover on the top of the consumable box.

[0021] On the basis of the above technical solution, preferably, the thermostat further comprises a heating module and a temperature control module, wherein:

[0022] The heating module is fixed at each side of the partition, and the heating module is electrically connected to the temperature control module;

[0023] The temperature control module is fixedly arranged in the operating area.

[0024] On the basis of the above technical solution, preferably, the constant temperature box also includes an ultraviolet lamp, wherein:

[0025] The ultraviolet lamp is respectively arranged one in the first buffer area, one in the second buffer area and one in the operation area;

[0026] The ultraviolet lamp is fixedly arranged on the T-shaped box cover.

[0027] On the basis of the above technical solution, preferably, the thermostat further includes an induction switch and a PLC controller, wherein:

[0028] The induction switch is fixedly arranged inside the first buffer zone and is used to control the start of the conveyor belt;

[0029] The PLC controller is fixedly arranged in the operation area, and the PLC controller is electrically connected to the induction switch and the conveyor belt.

[0030] On the basis of the above technical solution, preferably, the cover opening assembly includes a linear motor, a force arm, a vacuum suction cup and a vacuum pump, wherein:

[0031] The linear motor is fixedly arranged on one end of the operating area away from the consumables box, and the linear motor is electrically connected to the PLC controller, and the output direction of the linear motor is vertical;

[0032] One end of the force arm is fixedly arranged on the output end of the linear motor, and the other end is fixedly connected to the vacuum suction cup;

[0033] The vacuum suction cup is located above the conveyor belt, and the vacuum suction cup is connected to the vacuum pump through a pipeline;

[0034] The vacuum pump is fixedly arranged inside the operating area, and the vacuum pump is electrically connected to the PLC controller.

[0035] On the basis of the above technical solution, preferably, it also includes a button switch, wherein,

[0036] The button switch is arranged on the side end of the consumable box;

[0037] The button switches include a heating switch, an ultraviolet light switch, an arm lifting switch and a conveyor belt switch;

[0038] The heating switch is electrically connected to the heating module;

[0039] The ultraviolet lamp switch is electrically connected to the ultraviolet lamp;

[0040] The lever arm lifting switch is electrically connected to the PLC controller;

[0041] The conveyor belt switch is electrically connected to the conveyor belt.

[0042] On the basis of the above technical solution, preferably, the box body and the consumable box are transparent.

[0043] On the basis of the above technical solution, preferably, the liquid extraction nozzle is slidably connected to the corresponding universal ball, and the liquid addition nozzle is slidably connected to the corresponding universal ball.

[0044] On the basis of the above technical solution, preferably, a culture dish placement groove is provided on the conveyor belt surface of the conveyor belt.

[0045] Compared with the prior art, the cell culture device of the utility model has the following beneficial effects:

[0046] (1) A conveyor belt is provided to intermittently transport the culture dish, and a cover opening assembly is provided to open the dish cover. After opening, the culture fluid in the dish body is sucked out or new culture fluid is injected into the dish body through a liquid suction nozzle and a liquid addition nozzle. During the process, there is no need to manually open the covers one by one, which is convenient and quick, with less workload.

[0047] (2) By setting up a constant temperature box, it is convenient to create a relatively closed environment for fluid exchange, effectively protecting cells from contamination and damage.

[0048] (3) By setting a universal ball, and the liquid extraction nozzle and the liquid addition nozzle passing through the universal ball at the position, it is easy to adjust the use angle of the liquid extraction nozzle and the liquid addition nozzle and adjust the length of the nozzle extending into the constant temperature box, so as to facilitate the liquid replacement operation.

[0049] (4) By setting the box body and consumable box to be transparent, it is convenient to see objects and facilitate the liquid replacement operation.

[0050] (5) By setting a culture dish placement slot, it is convenient to limit the culture dishes placed on the conveyor belt, facilitate positioning and placement, and be relatively stable during transportation. BRIEF DESCRIPTION OF THE DRAWINGS

[0051] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0052] Figure 1 A three-dimensional diagram of a cell culture device of the utility model;

[0053] Figure 2 It is a schematic diagram of the internal structure of the thermostat of the utility model;

[0054] Figure 3 For the utility model Figure 2 AA section view;

[0055] Figure 4 For the utility model Figure 3 BB section view;

[0056] Figure 5 For the utility model Figure 4 CC section view;

[0057] Figure 6 This is a layout diagram of the push button switch of the utility model;

[0058] Figure 7It is a structural schematic diagram of the culture dish placement slot of the utility model;

[0059] Figure 8 It is the principle diagram of the utility model;

[0060] In the figure: 1. Constant temperature box; 2. Conveyor belt; 3. Opening cover assembly; 4. Universal ball; 5. Liquid extraction nozzle; 6. Liquid addition nozzle; 7. Push button switch; 11. Box body; 12. Partition; 13. Consumables box; 14. T-shaped box cover; 15. Heating module; 16. Temperature control module; 17. UV lamp; 18. Induction switch; 19. PLC controller; 31. Linear motor; 32. Force arm; 33. Vacuum suction cup; 34. Vacuum pump; 71. Heating switch; 72. UV lamp switch; 73. Force arm lifting switch; 74. Conveyor belt switch; 101. Culture dish entrance and exit channel; 102. First buffer zone; 103. Second buffer zone; 104. Operation area; 201. Culture dish placement slot. DETAILED DESCRIPTION

[0061] The following will be combined with the specific implementation of the utility model to clearly and completely describe the technical solution in the utility model. Obviously, the described implementation is only a part of the implementation of the utility model, not all of the implementations. Based on the implementation of the utility model, all other implementations obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0062] like Figure 1-8 As shown, a cell culture device of the utility model comprises a constant temperature box 1, a conveyor belt 2, a cover opening assembly 3, a universal ball 4, a liquid extraction nozzle 5 and a liquid addition nozzle 6, wherein a culture dish entrance and exit channel 101 is provided through the side end of the constant temperature box 1 in a horizontal direction for the culture dish to enter and exit the constant temperature box 1.

[0063] The thermostatic box 1 is a box with a constant temperature performance. Specifically, the thermostatic box 1 includes a box body 11 , a partition 12 , a consumable box 13 and a T-shaped box cover 14 .

[0064] Among them, two partitions 12 are fixedly set inside the box body 11, and the inside of the box body 11 is formed with a first buffer zone 102, a second buffer zone 103 and an operating zone 104 through the two partitions 12. The first buffer zone 102, the operating zone 104 and the second buffer zone 103 are arranged in sequence along the conveying direction of the conveyor belt 2. The culture dish entrance and exit channel 101 horizontally penetrates the box body 11 and the lower end of the partition 12. The cover opening assembly 3 is set in the operating zone 104, and the universal ball 4 is rotatably set on the T-shaped box cover 14 on the top of the consumable box 13. The culture dish entrance and exit channel 101 at one end of the first buffer zone 102 away from the operating zone 104 is the dish entrance, and the culture dish entrance and exit channel 101 at one end of the second buffer zone 103 away from the operating zone 104 is the dish exit.

[0065] The consumable box 13 is arranged on the side of the operation area 104 and is fixedly arranged on the side of the box body 11. The consumable box 13 is connected to the interior of the box body 11. A consumable tip area is arranged inside the consumable box 13. The consumable tip area is used to place a sterile tip box. The sterile tip box contains liquid extraction gun tips and liquid addition gun tips. By rotating the liquid extraction nozzle 5 and the liquid addition nozzle 6 and pressing them, the liquid extraction gun tips and the liquid addition gun tips can be plugged into the corresponding liquid extraction nozzle 5 and the liquid addition nozzle 6.

[0066] The T-shaped box cover 14 is detachably arranged on the top of the box body 11 and the consumable box 13. When the consumables need to be replaced, the T-shaped box cover 14 is opened, and the T-shaped box cover 14 is closed after replacement.

[0067] The thermostat 1 also includes a heating module 15 and a temperature control module 16, wherein a heating module 15 is fixed on each side of the partition 12, the heating module 15 and the temperature control module 16 are electrically connected, the temperature control module 16 is arranged in the operating area 104, and is fixed on the side end of one of the partitions 12, the temperature control module 16 is used to control the internal temperature of the thermostat 1, so that the temperature in the thermostat 1 is maintained at 36-37 degrees Celsius, and the temperature control circuit is any one of the prior art, such as the temperature control circuit of the thermostat disclosed in CN113885614A.

[0068] The constant temperature box 1 also includes an ultraviolet lamp 17, wherein one ultraviolet lamp 17 is arranged in each of the first buffer zone 102, the second buffer zone 103 and the operating zone 104, and the ultraviolet lamp 17 is fixedly arranged on the T-shaped box cover 14. The ultraviolet lamp 17 is used for sterilization in the box. The consumables pipette tip area is connected to the operating zone 104 without any partition, and shares one ultraviolet lamp 17. The consumables pipette tip area and the other three areas share the same openable T-shaped box cover 14.

[0069] In the above structure, the conveyor belt 2 is arranged inside the thermostatic box 1 and horizontally passes through the culture dish entrance and exit channel 101, and is used for intermittently conveying the culture dishes. The conveyor belt 2 is any intermittent conveyor in the prior art, such as an intermittent conveyor belt disclosed in CN219905720U. To realize automatic conveying control, the thermostatic box 1 also includes a sensor switch 18 and a PLC controller 19, wherein the sensor switch 18 is fixedly arranged inside the first buffer zone 102, and is used to control the start of the conveyor belt 2. The PLC controller 19 is fixedly arranged in the operating area 104. The PLC controller 19 is electrically connected to the sensor switch 18 and the conveyor belt 2. When the culture dish is placed on the conveyor belt 2, the sensor switch 18 senses the presence of the culture dish and gives an electrical signal to the PLC controller 19. After processing, the PLC controller 19 starts the conveyor belt 2 to run a distance, so that the culture dish moves to the bottom of the cover opening component 3.

[0070] In order to achieve the positioning and placement of the culture dish, a culture dish placement groove 201 is set on the conveyor belt surface of the conveyor belt 2. A plurality of culture dish placement grooves 201 are equidistantly arranged along the conveying direction of the conveyor belt 2. The distance between two adjacent culture dish placement grooves 201 is equal to the conveying distance of the conveyor belt 2 each time it rotates. When changing the liquid, the culture dish is placed in the culture dish placement groove 201. In order to further facilitate the positioning and placement of the culture dish, the two ends of the conveyor belt 2 extend outwardly to form a culture dish entry and exit channel 101.

[0071] In addition, a clamp structure can be set on the conveyor belt surface of the conveyor belt 2 to position and place the culture dish, such as using multiple spring sheets arranged in a ring. When placing the culture dish, the culture dish is clamped between the multiple spring sheets and fixed by the elastic force of the spring sheets.

[0072] The cover opening component 3 is arranged on one side of the conveyor belt 2 and is fixedly arranged inside the constant temperature box 1, and is used for opening the cover of the culture dish, and the cover opening direction is vertical. Specifically, the cover opening component 3 includes a linear motor 31, a force arm 32, a vacuum suction cup 33 and a vacuum pump 34, wherein the linear motor 31 is fixedly arranged on one end of the operation area 104 away from the consumable box 13, and the linear motor 31 is electrically connected to the PLC controller 19, the output direction of the linear motor 31 is vertical, one end of the force arm 32 is fixedly arranged on the output end of the linear motor 31, and the other end is fixedly connected to the vacuum suction cup 33, the vacuum suction cup 33 is above the conveyor belt 2, the vacuum suction cup 33 is connected to the vacuum pump 34 through a pipeline, and the vacuum pump 34 is fixedly arranged in the operation area 104. Internally, the vacuum pump 34 is electrically connected to the PLC controller 19. When the culture dish moves to the bottom of the vacuum suction cup 33, the PLC controller 19 starts the linear motor 31. The output end of the linear motor 31 drives the arm 32 to descend. The arm 32 drives the vacuum suction cup 33 to descend to the top of the dish cover and make contact. Then the PLC controller 19 starts the vacuum pump 34 to make the vacuum suction cup 33 vacuum-absorb the dish cover. Then the output end of the linear motor 31 drives the arm 32 to rise. The arm 32 drives the vacuum suction cup 33 to rise to open the cover. After opening the cover, the culture fluid is sucked or injected through the liquid suction nozzle 5 and the liquid addition nozzle 6. After changing the fluid, the output end of the linear motor 31 drives the arm 32 to descend again to cover the dish cover on the dish body, and then the vacuum suction cup 33 is lifted.

[0073] Two universal balls 4 are rotatably arranged on the top of the thermostat 1. One end of the liquid extraction nozzle 5 passes through the center of one of the universal balls 4 and extends to the inside of the thermostat 1. The other end is used to connect the suction pump, which is connected to the waste liquid bucket. One end of the liquid addition nozzle 6 passes through the center of another universal ball 4 and extends to the inside of the thermostat 1. The other end is used to connect the liquid addition pump, which is connected to the liquid storage tank. When the suction pump is working, the liquid in the culture dish can be extracted through the liquid extraction nozzle 5. When the liquid addition pump is working, the culture liquid can be injected into the culture dish through the liquid addition nozzle 6. The structure is used to extract liquid. The waste liquid is sucked so that the waste liquid reaches the waste liquid barrel directly without contacting the air and causing no aerosol pollution. At the same time, in this structure, the liquid extraction nozzle 5 and the corresponding universal ball 4 are connected by sliding along the axial direction of the liquid extraction nozzle 5, and the liquid adding nozzle 6 and the corresponding universal ball 4 are connected by sliding along the axial direction of the liquid adding nozzle 6. In this way, the length of the liquid extraction nozzle 5 and the liquid adding nozzle 6 extending into the box can be changed by sliding. By coordinating the rotation of the universal ball 4, the liquid extraction nozzle 5 and the liquid adding nozzle 6 are rotated and pressed, so that the liquid extraction gun head and the liquid adding gun head can be plugged into the nozzles of the corresponding liquid extraction nozzle 5 and the liquid adding nozzle 6.

[0074] To facilitate manual control, a cell culture device of the utility model further includes a button switch 7, which is installed on the side end of the consumable box 13, specifically, on the side end of the consumable box 13 away from the operation area 104, wherein the button switch 7 includes a heating switch 71, an ultraviolet lamp switch 72, a force arm lifting switch 73 and a conveyor belt switch 74, the heating switch 71 is electrically connected to the heating module 15, the ultraviolet lamp switch 72 is electrically connected to the ultraviolet lamp 17, the force arm lifting switch 73 is electrically connected to the PLC controller 19, and the conveyor belt switch 74 is electrically connected to the conveyor belt 2, and the corresponding components are controlled by the button switch 7. To facilitate the control of the vacuum pump 34, the button switch 7 also includes a vacuum pump switch, which is arranged between the ultraviolet lamp switch 72 and the conveyor belt switch 74, and the vacuum pump switch is electrically connected to the vacuum pump 34 for controlling the start and stop of the vacuum pump 34, and the vacuum pump switch is also installed on the side end of the consumable box 13.

[0075] In addition, the box body 11 and the consumable box 13 are transparent, which is convenient for viewing and for changing liquids.

[0076] Firstly, when a cell culture device of the utility model is used for changing the liquid of a cell culture dish, the experimenter does not need to manually open the culture dish to avoid contamination. The automatic opening area is in a closed area to avoid contamination. The suction pump has a short time and a fast speed for drawing and adding liquid, which reduces the influence of the external environment on cell growth. The experimenter only needs to place the dish at the entrance of the first buffer zone 102. After the induction switch 18 senses the culture dish, the conveyor belt 2 conveys the dish to the operation area 104. After the liquid change is completed, the conveyor belt 2 conveys the culture dish out of the operation area 104. The entire area can be thermostatically controlled to control the normal cell growth temperature of 37 degrees, thereby reducing the influence of the external environment on cell growth, improving efficiency and reducing the workload of the experimenter.

[0077] Secondly, after the work in the operation area 104 is completed, another dish is placed at the entrance of the first buffer area 102, and the conveyor belt 2 starts to operate to transport the processed dish to the second buffer area 103, and at the same time transport the next dish to be operated from the first buffer area 102 to the operation area 104, and this cycle is carried out to achieve continuous operation.

[0078] A method of using a cell culture device of the utility model is as follows:

[0079] S1. Before using the device, open the T-shaped box cover 14 for cleaning and disinfection, put the tip box in the consumable tip area, and do not open the T-shaped box cover 14 until the next group of cells are processed. Turn on the ultraviolet irradiation for 30 minutes;

[0080] S2. After the ultraviolet irradiation is finished, the heating switch 71 is turned on, the heating module 15 generates heat, and the temperature inside the box is controlled at 37 degrees Celsius;

[0081] S3, turn on the conveyor belt 2. At this time, the conveyor belt 2 will not run. When a culture dish is placed at the entrance of the first buffer zone 102, the conveyor belt 2 starts to move after the induction switch 18 senses it (the movement distance of the conveyor belt 2 each time has been set, that is, from one area to another area). When the culture dish reaches the operating area 104, the conveyor belt 2 stops moving.

[0082] S4. When the conveyor belt 2 is transporting the dishes, each nozzle can be manually moved to the tip box, and the corresponding gun tip can be sucked by manually pressing;

[0083] S5, when the culture dish reaches the operation area 104, the arm lifting switch 73 is used to control the arm 32 to move the vacuum suction cup 33 to the dish cover position, the vacuum pump 34 is turned on, the vacuum suction cup 33 sucks the dish cover, and the arm lifting switch 73 is used to control the arm 32 to move the dish cover to a certain height, such as 15 cm;

[0084] S6, manually rotate the universal ball 4 in the consumables suction head area, move the corresponding suction nozzle of the suctioned gun head to the culture dish, turn on the suction pump, and add liquid after the liquid is sucked. The liquid adding time can be set on the suction pump to control the amount of liquid added;

[0085] S7, after the operation of the dish in the operation area 104 is completed, another dish is placed at the entrance of the first buffer area 102, and the conveyor belt 2 starts to move, and the dish to be processed is transported from the first buffer area 102 to the operation area 104, and the dish processed in the operation area 104 is transported to the second buffer area 103, and the processed dish can be manually taken out from the exit of the second buffer area 103.

[0086] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A cell culture device, comprising a constant temperature box (1), characterized in that: It also includes a conveyor belt (2), a cover opening assembly (3), a universal ball (4), a liquid extraction nozzle (5) and a liquid addition nozzle (6), wherein: A culture dish access channel (101) is provided through the side end of the thermostatic box (1) in a horizontal direction; The conveyor belt (2) is arranged inside the constant temperature box (1) and horizontally penetrates the culture dish inlet and outlet channel (101) for intermittently conveying the culture dishes; The cover opening component (3) is arranged on one side of the conveyor belt (2) and is fixedly arranged inside the constant temperature box (1) and is used for opening the cover of the culture dish, and the cover opening direction is vertical; Two universal balls (4) are rotatably arranged on the top of the thermostatic box (1); One end of the liquid suction nozzle (5) passes through the center of one of the universal balls (4) and extends to the interior of the constant temperature box (1), and the other end is used to connect to a suction pump; One end of the liquid adding nozzle (6) passes through the center of the other universal ball (4) and extends to the interior of the constant temperature box (1), and the other end is used for connecting to the liquid adding pump.

2. A cell culture device according to claim 1, characterized in that: The constant temperature box (1) comprises a box body (11), a partition (12), a consumable box (13) and a T-shaped box cover (14), wherein: Two partitions (12) are fixedly arranged inside the box (11); a first buffer zone (102), a second buffer zone (103) and an operating zone (104) are formed inside the box (11) by the two partitions (12); the first buffer zone (102), the operating zone (104) and the second buffer zone (103) are arranged in sequence along the conveying direction of the conveyor belt (2); The consumables box (13) is arranged on the side of the operating area (104) and is fixedly arranged on the side of the box body (11); the consumables box (13) is connected to the inside of the box body (11); The T-shaped box cover (14) is detachably arranged on the top of the box body (11) and the consumable box (13); The culture dish access channel (101) horizontally penetrates the box (11) and the lower end of the partition (12); The cover opening component (3) is arranged in the operating area (104); The universal ball (4) is rotatably arranged on the T-shaped box cover (14) on the top of the consumable box (13).

3. A cell culture device according to claim 2, characterized in that: The thermostatic box (1) further comprises a heating module (15) and a temperature control module (16), wherein: The heating module (15) is fixed at each side of the partition (12), and the heating module (15) is electrically connected to the temperature control module (16); The temperature control module (16) is fixedly arranged in the operating area (104).

4. A cell culture device according to claim 3, characterized in that: The thermostatic box (1) further comprises an ultraviolet lamp (17), wherein: The ultraviolet lamp (17) is provided one each in the first buffer area (102), the second buffer area (103) and the operating area (104); The ultraviolet lamp (17) is fixedly arranged on the T-shaped box cover (14).

5. A cell culture device according to claim 4, characterized in that: The thermostatic box (1) further comprises an induction switch (18) and a PLC controller (19), wherein: The induction switch (18) is fixedly arranged inside the first buffer zone (102) and is used to control the start of the conveyor belt (2); The PLC controller (19) is fixedly arranged in the operating area (104), and the PLC controller (19) is electrically connected to the induction switch (18) and the conveyor belt (2).

6. A cell culture device according to claim 5, characterized in that: The lid opening assembly (3) comprises a linear motor (31), a force arm (32), a vacuum suction cup (33) and a vacuum pump (34), wherein: The linear motor (31) is fixedly arranged on one end of the operating area (104) away from the consumable box (13), and the linear motor (31) is electrically connected to the PLC controller (19), and the output direction of the linear motor (31) is vertical; One end of the force arm (32) is fixedly arranged on the output end of the linear motor (31), and the other end is fixedly connected to the vacuum suction cup (33); The vacuum suction cup (33) is located above the conveyor belt (2), and the vacuum suction cup (33) is connected to the vacuum pump (34) through a pipeline; The vacuum pump (34) is fixedly arranged inside the operating area (104), and the vacuum pump (34) is electrically connected to the PLC controller (19).

7. A cell culture device according to claim 6, characterized in that: It also includes a push button switch (7), wherein: The button switch (7) is arranged on the side end of the consumable box (13); The button switch (7) comprises a heating switch (71), an ultraviolet lamp switch (72), a force arm lifting switch (73) and a conveyor belt switch (74); The heating switch (71) is electrically connected to the heating module (15); The ultraviolet lamp switch (72) is electrically connected to the ultraviolet lamp (17); The arm lifting switch (73) is electrically connected to the PLC controller (19); The conveyor belt switch (74) is electrically connected to the conveyor belt (2).

8. A cell culture device according to claim 2 or 7, characterized in that: The box body (11) and the consumables box (13) are transparent.

9. A cell culture device according to claim 1, characterized in that: The liquid extraction nozzle (5) is slidably connected to the corresponding universal ball (4), and the liquid addition nozzle (6) is slidably connected to the corresponding universal ball (4).

10. A cell culture device according to claim 1 or 7, characterized in that: A culture dish placement groove (201) is provided on the conveyor belt surface of the conveyor belt (2).

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