Affinity column uncapping mechanism

Abstract

The utility model provides an affinity column capping mechanism, comprising a base, a driving mechanism being arranged in the base, a lifting shell being sleeved on the base, a screw rod of the driving mechanism passing through the base and connected with the lifting shell, a group of control components being arranged on the same side of the lifting shell and the base, a capping device being arranged on the lifting shell, and the top end of the capping device being connected with a cap collecting box through a cap guide groove. The utility model solves the problems of inconvenient manual operation of capping of immunoaffinity columns, splashing of chemical reagents, repeated operation and low efficiency.

Description

Technical Field

[0001] The utility model relates to the technical field of experimental equipment, in particular to an affinity column cap removal mechanism. Background Art

[0002] The full name of immunoaffinity column is immunoaffinity chromatography column, which is often referred to as affinity column, immunoaffinity column, affinity chromatography column, etc. The principle of immunoaffinity column is to covalently bind the antibody to the inert microbeads, make an affinity adsorbent and then load it into the column, then pass the antigen solution through the immunoaffinity column, while the non-target compounds flow down the column, and finally elute the antigen with elution buffer to obtain the purified antigen. Using the high specificity of immunoaffinity column, pure sample extract can be obtained by purification using immunoaffinity column.

[0003] Since the washing and elution process of the immunoaffinity column requires cap removal, which takes a long time, especially when the sample size is large, repeated operations are required for a long time, which not only increases the labor intensity of the operators but also leads to a decrease in work efficiency and cannot meet the needs of mass production. Utility Model Content

[0004] The technical problem to be solved by the utility model is that the washing and elution process of the immunoaffinity column requires cap removal, which takes a relatively long time. Especially when the sample volume is relatively large, long-term repeated operations are required, which not only increases the labor intensity of the operators but also leads to a decrease in work efficiency and cannot meet the needs of mass production.

[0005] In order to solve the above technical problems, the utility model provides an affinity column capping mechanism, including a base, a driving mechanism is arranged in the base, a lifting shell is sleeved on the base, a screw rod of the driving mechanism passes through the base and is connected to the lifting shell, a baffle is arranged on one side of the lifting shell, the baffle is provided with a limit bolt, the limit bolt passes through the lifting shell and is inserted into the notch of the base and moves in the notch of the base to limit the movement of the lifting shell. A group of control components is arranged on the base on the same side as the baffle, a capping device is arranged on the lifting shell, and the top end of the capping device is connected to the cap collection box through a cap guide groove.

[0006] Further, the driving mechanism includes a screw motor, and the lifting shell is sleeved on the screw of the screw motor and connected to the lifting shell.

[0007] Furthermore, a rubber pad is provided between the cap removing device and the lifting shell.

[0008] Furthermore, the control component includes a photoelectric switch, which is arranged on one side of the base, and the photoelectric switch corresponds to the baffle one by one.

[0009] Furthermore, the capping device comprises a capping hook bracket, the top of which is connected to a capping hook, an immunoaffinity column is arranged on the capping hook, and a capping guide groove is connected to the capping hook.

[0010] Furthermore, one end of the cap guide groove connected to the cap removal hook is an inclined section opening, and the cap removal hook passes through the bottom surface of the cap guide groove opening.

[0011] Furthermore, the upper two sides of the decapping hook are at right angles, the opening diameter of the top of the decapping hook is smaller than the cap diameter and larger than the diameter of the immunoaffinity column, and the inner diameter of the middle of the decapping hook is larger than the cap diameter.

[0012] Furthermore, the internal space of the lifting shell is larger than the volume of the driving mechanism, and the external shape of the lifting shell is a cube, a cylinder or a trapezoid.

[0013] Advantages of this utility model:

[0014] The utility model solves the problem that the washing and elution processes of the immunoaffinity column require a relatively long time for cap removal, especially when the sample volume is relatively large, and long-term repeated operations are required, which not only increases the labor intensity of the operators but also leads to a decrease in work efficiency and cannot meet the needs of mass production.

[0015] The utility model realizes the process of rapid and automatic cap removal through a driving mechanism, a lifting shell, a cap removal device, a control component, a cap guide groove and a cap collection box. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a three-dimensional diagram of an affinity column uncapping mechanism.

[0017] Figure 2 A side planing view of an affinity column uncapping mechanism.

[0018] Figure 3 This is a side planing view of a cap removal device in an affinity column cap removal mechanism.

[0019] Figure 4 The present invention is a structural diagram of a control component in a cap removal device in an affinity column cap removal mechanism.

[0020] Figure 5 The present invention is a control diagram of a cap removal device in an affinity column cap removal mechanism.

[0021] As shown in the figure:

[0022] 1. Base; 2. Driving mechanism; 3. Lifting shell; 4. Rubber pad; 5. Cap removal hook bracket; 6. Cap guide groove; 7. Cap removal hook; 8. Immunoaffinity column; 9. Cap collection box; 10. Cap; 11. Photoelectric switch; 12. Limit bolt; 13. Cap removal device; 14. Screw; 15. Control component; 16. Baffle; 17. Notch; 18. Main control board; 19. Stepper motor driver. DETAILED DESCRIPTION

[0023] The specific implementation of the utility model is further described below in conjunction with the accompanying drawings, wherein the same parts are represented by the same reference numerals.

[0024] It should be noted that the words "front", "rear", "left", "right", "up" and "down" used in the following description refer to directions in the drawings, and the words "inside" and "outside" refer to directions toward or away from the geometric center of a specific component, respectively.

[0025] In order to make the contents of the present invention more clearly understood, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention.

[0026] Combined with Figure 1-3 As shown, in order to solve the above technical problems, the utility model provides an affinity column capping mechanism, including a base 1, a driving mechanism 2 is provided in the base 1, a lifting shell 3 is sleeved on the base 1, and a screw rod 14 of the driving mechanism 2 passes through the base 1 and is connected to the lifting shell 3.

[0027] The lifting housing 3 is threadedly connected to the screw rod 14. When the driving mechanism 2 drives the screw rod 14 to rotate, the lifting housing 3 moves in the direction of the screw rod 14. The driving mechanism 2 includes a screw motor. The lifting housing 3 is sleeved on the screw rod 14 of the screw motor and connected to the lifting housing 3.

[0028] A baffle 16 is provided on one side of the lifting shell 3, and a limiting bolt 12 is provided on the baffle 16. The limiting bolt 12 passes through the lifting shell 3 and is inserted into the slot 17 of the base 1 and moves in the slot 17 of the base 1 to limit the movement of the lifting shell.

[0029] A group of control components 15 is provided on the base 1 on the same side as the baffle 16 .

[0030] The control assembly 15 controls the rotation direction of the driving mechanism 2, thereby controlling the lifting movement of the lifting housing 3.

[0031] The lifting shell 3 is provided with a cap removing device 13 , and the top end of the cap removing device 13 is connected to the cap collecting box 9 through the cap guide groove 6 .

[0032] When the lifting shell 3 drives the cap removing device 13 to move up and down, and then the caps are pulled, the caps enter the cap collecting box 9 through the cap guide grooves 6.

[0033] The utility model solves the problem that the washing and elution processes of the immunoaffinity column require a relatively long time for cap removal, especially when the sample volume is relatively large, and long-term repeated operations are required, which not only increases the labor intensity of the operators but also leads to a decrease in work efficiency and cannot meet the needs of mass production.

[0034] A rubber pad 4 is provided between the cap removing device 13 and the lifting shell 3 .

[0035] The rubber pad 4 plays a sealing role to prevent liquid from flowing into the screw motor through the gap between the lifting shell 3 and the cap-removing hook bracket 5.

[0036] The control component 15 includes a photoelectric switch 11, a baffle 16 and a limit bolt 12. The photoelectric switch 11 is arranged on one side of the base 1. The baffle 16 is arranged on the same side of the lifting shell 3 corresponding to the photoelectric switch 11. The baffle 16 is provided with a limit bolt 12. The photoelectric switch 11 corresponds to the baffle one by one.

[0037] The photoelectric switch is electrically connected to the driving mechanism 2 .

[0038] When the photoelectric switch 11, the baffle 16, and the limit bolt 12 form a control assembly 15 on the same side, when the lifting housing 3 descends, the baffle 16 is driven down, and the photoelectric switch 11 is triggered by the baffle 16, thereby controlling the forward or reverse movement of the driving mechanism 2, and the movement of the lifting housing 3 is controlled by the forward and reverse movement of the driving mechanism 2. The baffle 16 is limited by the limit bolt 12 to prevent the baffle 16 from descending and causing damage to the photoelectric switch 11.

[0039] The decapping device 13 comprises a decapping hook bracket 5 , the top end of which is connected to a decapping hook 7 , an immunoaffinity column 8 is arranged on the decapping hook 7 , and a capping guide groove 6 is connected to the decapping hook 7 .

[0040] One end of the cap guide slot 6 connected to the cap removal hook 7 is an inclined cut surface opening, and the cap removal hook 7 passes through the bottom surface of the opening of the cap guide slot 6. This can prevent the cap from falling out of the cap guide slot 6.

[0041] The upper two sides of the decapping hook 7 are at right angles, the top opening diameter of the decapping hook 7 is smaller than the diameter of the cap 10 and larger than the diameter of the immunoaffinity column 8 , and the inner diameter of the middle part of the decapping hook 7 is larger than the diameter of the cap 10 .

[0042] The cap is in the cap-removing hook 7, and the cap can be quickly removed by pulling down the cap-removing hook 7.

[0043] The internal space of the lifting shell 3 is larger than the volume of the driving mechanism 2 , and the external shape of the lifting shell 3 is a cube, a cylinder or a trapezoid.

[0044] The screw motor is fixed on the base 1, and the lifting shell 3 is fixed by the screw nut and then installed on the screw 14 of the screw motor. The cap removal hook 7 and the cap guide groove 6 are fixed on the cap removal hook bracket 5, and the cap removal hook bracket 5 is fixed to the lifting shell 3. A rubber pad 4 is installed between the cap removal hook bracket 5 and the lifting shell 3. During operation, the immunoaffinity column 8 passes through the cap removal hook 7. When the immunoaffinity column 8 moves to the position of the cap removal hook 7, the screw motor 2 pulls the fixed shell 3 downward through the screw nut, and at the same time, the cap removal hook 7 separates the cap 10 from the immunoaffinity column 8. The cap 10 slides into the cap collection box 9 through the cap guide groove 6. When the baffle 16 on the lifting shell 3 moves to the photoelectric switch 11, the screw motor 2 stops running and runs in the reverse direction to the initial position to complete the cap removal work.

[0045] The main control board 18 sends a cap removal instruction to the stepper motor driver 19, and the stepper motor driver 19 drives the screw stepper motor to run, and the screw stepper motor drives the cap removal mechanism 7 to move downward. When the baffle 16 drops to the photoelectric switch 11, the photoelectric switch 11 is triggered, and the photoelectric switch 11 sends a signal to the main control board 18. After receiving the signal, the main control board 18 sends stop and reverse operation instructions to the stepper motor driver 19 in turn. The stepper motor driver 19 stops the screw stepper motor and then runs in reverse, so that the hair removal mechanism 7 moves upward to complete the cap removal movement.

[0046] The standard parts used in the utility model can be purchased from the market, and special-shaped parts can be customized according to the description in the specification and the drawings. The specific connection methods of each part adopt the conventional means such as mature bolts, rivets, welding, etc. in the prior art. The machinery, parts and equipment all adopt the conventional models in the prior art, and the circuit connection adopts the conventional connection method in the prior art, which will not be described in detail here. The content not described in detail in this specification belongs to the prior art known to professional and technical personnel in this field.

[0047] The above description of the utility model and its implementation methods is not restrictive. The drawings show only one implementation method of the utility model, and the actual structure is not limited thereto. In short, if ordinary technicians in this field are inspired by it and design structural methods and embodiments similar to the technical solution without creativity without departing from the purpose of the invention of the utility model, they should all fall within the protection scope of the utility model.

Claims

1. An affinity column uncapping mechanism, characterized in that: The invention comprises a base (1), a driving mechanism (2) is arranged inside the base (1), a lifting shell (3) is sleeved on the base (1), and a screw rod (14) of the driving mechanism (2) passes through the base (1) and is connected to the lifting shell (3); A baffle (16) is provided on one side of the lifting shell (3), and a limiting bolt (12) is provided on the baffle (16). The limiting bolt (12) passes through the lifting shell (3) and is inserted into a notch (17) of the base (1) and moves in the notch (17) of the base (1) to limit the movement of the lifting shell; A control assembly (15) is provided on the base (1) on the same side as the baffle (16), and a cap removal device (13) is provided on the lifting shell (3). The top end of the cap removal device (13) is connected to the cap collection box (9) through a cap guide groove (6).

2. An affinity column decapping mechanism according to claim 1, characterized in that: The driving mechanism (2) comprises a screw motor, and the lifting housing (3) is sleeved on a screw (14) of the screw motor and connected to the lifting housing (3).

3. The affinity column decapping mechanism according to claim 1, characterized in that: A rubber pad (4) is provided between the cap removing device (13) and the lifting shell (3).

4. The affinity column decapping mechanism according to claim 1, characterized in that: The control component (15) comprises a photoelectric switch (11), wherein the photoelectric switch (11) is arranged on one side of the base (1), and the photoelectric switch (11) corresponds to the baffle (16) in one-to-one correspondence.

5. An affinity column decapping mechanism according to claim 1 or 3, characterized in that: The decapping device (13) comprises a decapping hook bracket (5), the top end of the decapping hook bracket (5) is connected to a decapping hook (7), an immunoaffinity column (8) is arranged on the decapping hook (7), and the decapping hook (7) is connected to a capping guide groove (6).

6. The affinity column decapping mechanism according to claim 5, characterized in that: One end of the cap guide groove (6) connected to the cap removal hook (7) is an inclined cut surface opening, and the cap removal hook (7) passes through the bottom surface of the opening of the cap guide groove (6).

7. An affinity column decapping mechanism according to any one of claims 1 to 3, characterized in that: The internal space of the lifting shell (3) is larger than the volume of the driving mechanism (2), and the external shape of the lifting shell (3) is a cube, a cylinder or a trapezoid.

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