Single molecule optical detection module

Abstract

The utility model relates to the technical field of single-molecule optical detection, and discloses a single-molecule optical detection module, including a base plate, the top of which is provided with a laser, an attenuation plate, a first objective lens, a sheath flow cell, a second objective lens, a lens assembly and a PMT, which are sequentially connected to form an optical path, an APD fixing plate is provided on the other side of the sheath flow cell, and an APD is provided on the APD fixing plate, the APD and the PMT are arranged in parallel, and the laser is perpendicular to the horizontal line where the PMT and the APD are located. The utility model provides an integrated optical detection module, which analyzes the sample by capturing the fluorescence and scattered light passing through the sample in the sheath flow cell, simplifies the overall design and debugging of the optical path, and can achieve high-sensitivity detection of low-abundance samples, solving the problem in the prior art that the optical system is mostly constructed of multiple scattered parts, which may have errors in the assembly of parts, and there may be debugging differences during each debugging process, which is not conducive to system debugging and modular production.

Description

Technical Field

[0001] The utility model relates to the technical field of single molecule optical detection, in particular to a single molecule optical detection module. Background Art

[0002] Single-molecule immunoassays can detect proteins in serum, plasma, tears and other body fluids with ultra-high sensitivity (up to 10-18 mol / L) by limiting the molecules to be detected within a very small space (below nanoliters) and performing absolute counting of the detection signals. It has a wide range of applications in clinical and scientific research, such as:

[0003] (1) It helps to accurately analyze ultra-low concentration biomarkers related to various diseases, including autoimmune diseases, cardiovascular diseases, neurological diseases, and tumor diagnosis and treatment. Especially in the study of neurological diseases, high-sensitivity protein detection methods can accurately detect ultra-trace amounts of neurological disease markers in the blood, which can be used to assist in the early screening, diagnosis and efficacy evaluation of neurological diseases (such as Alzheimer's disease).

[0004] (2) Inflammatory factors in trace samples such as aqueous humor, vitreous body, and tears can be detected by increasing the dilution multiple.

[0005] (3) Protein can be quantified in single cells, enabling protein detection in the supernatant of single embryonic cell culture.

[0006] (4) Biomarker analysis, biopharmaceutical research, analytical development, clinical sample testing, and accurate data delivery.

[0007] Therefore, single-molecule immunoassay products will replace the current mainstream detection technology to a certain extent in the future, and will discover more clinically valuable biomarkers for the diagnosis and treatment of patients.

[0008] Most existing optical systems are built up of multiple scattered parts. Since there may be errors in the assembly of parts and there may be debugging differences during each debugging process, it is not conducive to system debugging and modular production. Utility Model Content

[0009] The utility model aims to solve the problems existing in the prior art and proposes a single molecule optical detection module.

[0010] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0011] A single-molecule optical detection module comprises a base plate, on the top of which are arranged a laser, an attenuation plate, a first objective lens, a sheath flow cell, a second objective lens, a lens assembly and a PMT which are sequentially connected to form an optical path; an APD fixing plate is arranged on the other side of the sheath flow cell, and an APD is arranged on the APD fixing plate; the APD and the PMT are arranged in parallel, and the laser is perpendicular to the horizontal line where the PMT and the APD are located.

[0012] Preferably, the lens assembly comprises a filter and a convex lens which are arranged in sequence.

[0013] Preferably, the laser output direction of the first objective lens is perpendicular to the laser input direction of the second objective lens.

[0014] Preferably, a laser driver and an optical path control board are also arranged on the bottom plate, and the laser driver and the optical path control board are arranged on one side of the PMT, and the laser driver is located between the optical path control board and the PMT.

[0015] Preferably, a power adapter and a transformer are also provided on the bottom plate. The power adapter is provided between the laser and the optical path control board, and the transformer is provided on a side of the laser away from the attenuation plate.

[0016] Preferably, a PMT high voltage module is also provided on the bottom plate, and the PMT high voltage module is provided on one side of the PMT.

[0017] Preferably, a fan is provided on the top of the PMT, and the fan is connected to the PMT via a heat sink.

[0018] Compared with the prior art, the beneficial effects of the utility model are:

[0019] The utility model connects a laser, an attenuation plate, a first objective lens, a sheath flow cell, a second objective lens, a lens assembly and a PMT in sequence to form an optical path. Meanwhile, the PMT cooperates with the APD to respectively receive a scattered light signal and a fluorescence signal. The module has a simple structure and a high degree of integration. Compared with the prior art, the utility model provides an integrated optical detection module. By capturing the fluorescence and scattered light of the sample passing through the sheath flow cell, the sample is analyzed, the overall design and debugging of the optical path are simplified, modular production is realized, and high-sensitivity detection of low-abundance samples can be achieved. The problem in the prior art that the optical system is mostly constructed of multiple scattered parts, errors may exist in the assembly of parts, and debugging differences may exist in each debugging process, which is not conducive to system debugging and modular production, is solved. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 This is a three-dimensional schematic diagram of the single-molecule optical detection module proposed by the utility model;

[0021] Figure 2 This is a front view of the single-molecule optical detection module proposed by the utility model;

[0022] Figure 3 This is a side view of the single-molecule optical detection module proposed by the utility model;

[0023] Figure 4 This is a top view of the single-molecule optical detection module proposed in the present invention.

[0024] In the figure: 1. base plate; 2. laser; 3. attenuation plate; 4. first objective lens; 5. second objective lens; 6. lens assembly; 7. PMT; 8. APD fixing plate; 9. fan; 10. heat sink; 11. PMT high voltage module; 12. laser driver; 13. optical path control board; 14. power adapter; 15. sheath flow cell; 16. transformer; 17. APD. DETAILED DESCRIPTION

[0025] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments.

[0026] Reference Figure 1-Figure 4 The single-molecule optical detection module includes a base plate 1, and the top of the base plate 1 is provided with a laser 2, an attenuation plate 3, a first objective lens 4, a sheath flow cell 15, a second objective lens 5, a lens assembly 6 and a PMT7 which are sequentially connected to form an optical path. An APD fixing plate 8 is provided on the other side of the sheath flow cell 15, and an APD17 is provided on the APD fixing plate 8. The APD17 and PMT7 are arranged in parallel, and the laser 2 is perpendicular to the horizontal line where the PMT7 and the APD17 are located.

[0027] When this module is in use, the laser 2, the attenuation plate 3, the first objective lens 4, the sheath flow cell 15, the second objective lens 5, the lens assembly 6 and the PMT7 are connected in sequence to form an optical path. At the same time, the PMT7 cooperates with the APD17 to receive the scattered light signal and the fluorescence signal respectively. The module has a simple structure and a high degree of integration. Compared with the prior art, the utility model provides an integrated optical detection module. By capturing the fluorescence and scattered light of the sample passing through the sheath flow cell 15, the sample is analyzed, the overall design and debugging of the optical path are simplified, modular production is realized, and high-sensitivity detection of low-abundance samples can be achieved. The problem in the prior art that the optical system is mostly constructed of multiple scattered parts, and there may be errors in the assembly of parts and there may be debugging differences in each debugging process, which is not conducive to system debugging and modular production.

[0028] In this embodiment, the lens assembly 6 includes a filter and a convex lens which are arranged in sequence. The filter is used to filter invisible light such as infrared and ultraviolet rays, and the convex lens is used to concentrate the light.

[0029] In this embodiment, the laser output direction of the first objective lens 4 is perpendicular to the laser input direction of the second objective lens 5. Figure 1 It can be seen that the first objective lens 4 inputs the laser into the sheath flow cell 15 from the horizontal direction, and the second objective lens 5 receives the output light of the sheath flow cell 15 from the vertical direction. This optical path design makes it easier for PMT7 and APD17 to capture the fluorescence and scattered light of the sample in the sheath flow cell 15 respectively.

[0030] In this embodiment, a laser driver 12 and an optical path control board 13 are also provided on the base plate 1. The laser driver 12 and the optical path control board 13 are arranged on one side of the PMT 7, and the laser driver 12 is located between the optical path control board 13 and the PMT 7. The laser driver 12 is used to connect the laser 2 with the power supply, to provide power supply and drive current control, to control the opening and closing of the laser 2, and to realize functions such as optical power regulation. The optical path control board 13 is used to control the working state of the laser 2 and the adjustment of various parameters.

[0031] In this embodiment, a power adapter 14 and a transformer 16 are also provided on the base plate 1. The power adapter 14 is arranged between the laser 2 and the optical path control board 13, and the transformer 16 is arranged on the side of the laser 2 away from the attenuation plate 3. The power adapter 14 converts AC power into DC power suitable for use in this module. External AC power can be directly connected to this module through a plug and a connecting line, and the transformer 16 is used to adjust the voltage.

[0032] In this embodiment, a PMT high voltage module 11 is further provided on the bottom plate 1. The PMT high voltage module 11 is provided on one side of the PMT 7. The PMT high voltage module 11 is used in conjunction with the PMT 7 to boost the voltage thereof.

[0033] In this embodiment, a fan 9 is provided on the top of the PMT 7, and the fan 9 is connected to the PMT 7 through a heat sink 10, so as to dissipate heat for the PMT 7 and increase the service life of the PMT 7.

[0034] Working principle: When the laser 2 is working, it emits laser, which passes through the attenuation plate 3, the first objective lens 4, the sheath flow cell 15, the second objective lens 5, the lens assembly 6 and the PMT7 in sequence. During this process, the PMT7 cooperates with the APD17 to receive the scattered light signal and the fluorescence signal respectively, capture the fluorescence and scattered light passing through the sample in the sheath flow cell 15, and analyze the sample. Compared with the prior art, the utility model provides an integrated optical detection module, which analyzes the sample by capturing the fluorescence and scattered light passing through the sample in the sheath flow cell 15, simplifies the overall design and debugging of the optical path, realizes modular production, and can achieve high-sensitivity detection of low-abundance samples, solving the problem in the prior art that the optical system is mostly constructed of multiple scattered parts, which may have errors in the assembly of parts and may have debugging differences during each debugging process, which is not conducive to system debugging and modular production.

[0035] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes within the technical scope disclosed by the present invention according to the technical scheme and the utility model concept of the present invention, which should be covered by the protection scope of the present invention.

Claims

1. Single molecule optical detection module, characterized in that: The invention comprises a base plate (1), wherein a laser (2), an attenuation plate (3), a first objective lens (4), a sheath flow cell (15), a second objective lens (5), a lens assembly (6) and a PMT (7) which are sequentially connected to form an optical path are arranged on the top of the base plate (1), an APD fixing plate (8) is arranged on the other side of the sheath flow cell (15), and an APD (17) is arranged on the APD fixing plate (8), the APD (17) and the PMT (7) are arranged in parallel, and the laser (2) is perpendicular to the horizontal line where the PMT (7) and the APD (17) are located.

2. The single-molecule optical detection module according to claim 1, characterized in that: The lens assembly (6) comprises a filter and a convex lens which are arranged in sequence.

3. The single-molecule optical detection module according to claim 1, characterized in that: The laser output direction of the first objective lens (4) is perpendicular to the laser input direction of the second objective lens (5).

4. The single-molecule optical detection module according to claim 2, characterized in that: A laser driver (12) and an optical path control board (13) are also arranged on the bottom plate (1); the laser driver (12) and the optical path control board (13) are arranged on one side of the PMT (7), and the laser driver (12) is located between the optical path control board (13) and the PMT (7).

5. The single-molecule optical detection module according to claim 1, characterized in that: A power adapter (14) and a transformer (16) are also provided on the base plate (1); the power adapter (14) is provided between the laser (2) and the optical path control board (13); and the transformer (16) is provided on a side of the laser (2) away from the attenuation plate (3).

6. The single-molecule optical detection module according to claim 1, characterized in that: A PMT high-voltage module (11) is also provided on the bottom plate (1), and the PMT high-voltage module (11) is provided on one side of the PMT (7).

7. The single-molecule optical detection module according to claim 6, characterized in that: A fan (9) is arranged on the top of the PMT (7), and the fan (9) is connected to the PMT (7) via a heat sink (10).

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